WO2010093174A3 - 토마토 히스티딘 디카르복실라제 유전자 유래 열매 특이적 발현 프로모터 및 이의 용도 - Google Patents

토마토 히스티딘 디카르복실라제 유전자 유래 열매 특이적 발현 프로모터 및 이의 용도 Download PDF

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Publication number
WO2010093174A3
WO2010093174A3 PCT/KR2010/000849 KR2010000849W WO2010093174A3 WO 2010093174 A3 WO2010093174 A3 WO 2010093174A3 KR 2010000849 W KR2010000849 W KR 2010000849W WO 2010093174 A3 WO2010093174 A3 WO 2010093174A3
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WIPO (PCT)
Prior art keywords
gene
fruit
specific expression
derived
solanum lycopersicum
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PCT/KR2010/000849
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English (en)
French (fr)
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WO2010093174A2 (ko
Inventor
정영희
김아영
이상협
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전남대학교산학협력단
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Priority to US13/148,949 priority Critical patent/US8729340B2/en
Priority to CN2010800076744A priority patent/CN102317456B/zh
Publication of WO2010093174A2 publication Critical patent/WO2010093174A2/ko
Publication of WO2010093174A3 publication Critical patent/WO2010093174A3/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8235—Fruit-specific

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Reproductive Health (AREA)
  • Pregnancy & Childbirth (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Physiology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

본 발명은 토마토 (Solanum lycopersicum)의 히스티딘 디카르복실라제-2 (Histidine decarboxylase-2; SlHD-2) 유전자 유래 식물 열매 특이적 발현 프로모터 및 5' 비번역 영역 (untranslated region; 5'-UTR), 이를 포함하는 식물 열매 특이적 발현 벡터, 상기 발현 벡터를 이용하여 외래 유전자를 열매 특이적으로 발현시키는 방법, 상기 발현 벡터로 형질전환된 식물체 및 이의 종자에 관한 것이다. 본 발명에 따르면, 기존에 널리 사용되고 있는 꽃양배추 모자이크 바이러스 유래의 CaMV 35S 프로모터가 전 조직에서 외래 유전자의 발현을 유도하는 것에 비해 형질전환 식물체에서 도입된 유전자를 열매 조직 특이적으로 발현시킬 수 있다. 또한 본 발명은 유용물질을 열매에서 생산하고자 하는 형질전환 식물체 개발에 유용하게 이용될 수 있다.
PCT/KR2010/000849 2009-02-12 2010-02-11 토마토 히스티딘 디카르복실라제 유전자 유래 열매 특이적 발현 프로모터 및 이의 용도 WO2010093174A2 (ko)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US13/148,949 US8729340B2 (en) 2009-02-12 2010-02-11 Solanum lycopersicum histidine decarboxylase gene-derived fruit-specific expression promoter and uses thereof
CN2010800076744A CN102317456B (zh) 2009-02-12 2010-02-11 源自番茄组氨酸脱羧酶基因的果实特异性表达启动子及其用途

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2009-0011585 2009-02-12
KR1020090011585A KR101101774B1 (ko) 2009-02-12 2009-02-12 토마토 히스티딘 디카르복실라제 유전자 유래 열매 특이적 발현 프로모터 및 이의 용도

Publications (2)

Publication Number Publication Date
WO2010093174A2 WO2010093174A2 (ko) 2010-08-19
WO2010093174A3 true WO2010093174A3 (ko) 2011-01-06

Family

ID=42562180

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2010/000849 WO2010093174A2 (ko) 2009-02-12 2010-02-11 토마토 히스티딘 디카르복실라제 유전자 유래 열매 특이적 발현 프로모터 및 이의 용도

Country Status (4)

Country Link
US (1) US8729340B2 (ko)
KR (1) KR101101774B1 (ko)
CN (1) CN102317456B (ko)
WO (1) WO2010093174A2 (ko)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR102012008162B1 (pt) * 2012-04-09 2018-08-14 Empresa Brasileira De Pesquisa Agropecuária - Embrapa composições e métodos para modificar a expressão de genes de interesse

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL8300698A (nl) 1983-02-24 1984-09-17 Univ Leiden Werkwijze voor het inbouwen van vreemd dna in het genoom van tweezaadlobbige planten; agrobacterium tumefaciens bacterien en werkwijze voor het produceren daarvan; planten en plantecellen met gewijzigde genetische eigenschappen; werkwijze voor het bereiden van chemische en/of farmaceutische produkten.
NZ224787A (en) * 1987-05-26 1990-08-28 Calgene Inc A dna construct for specifically modifying the phenotype of fruit as distinct from other plant tissue
CA1327173C (en) 1987-07-21 1994-02-22 Erwin Heberle-Bors Method of gene transfer into plants
US5723746A (en) * 1989-12-12 1998-03-03 Epitope, Inc. Reduced ethylene synthesis and delayed fruit ripening in transgenic tomatoes expressing S-adenosylmethionine hydrolase
EP0638120A1 (en) * 1991-10-04 1995-02-15 Smart Plants International, Inc. Tissue-specific and developmentally regulated transcriptional sequences and uses thereof
US5536653A (en) * 1994-11-04 1996-07-16 Monsanto Company Tomato fruit promoters
KR100574563B1 (ko) 2003-10-10 2006-04-27 고려대학교 산학협력단 애기장대 유래 식물체 고효율 발현 프로모터 및 이를함유하는 식물체 고효율 발현 벡터
KR100784165B1 (ko) 2006-06-19 2007-12-10 고려대학교 산학협력단 고추식물 유래의 ｕｄｐ-글루코실트랜스퍼라제의 프로모터

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* Cited by examiner, † Cited by third party
Title
KORBAN ET AL.: "Foods as production and delivery vehicles for human vaccines", J OF AMERICAN COLLEGE OF NUTRITION, vol. 21, no. 3, 2002, pages 212S - 217S *
KRASNYANSKI ET AL.: "Effect of an enhanced CaMV 35S promoter and a fruit-specific promoter on UIDA gene expression in transgenic tomato plants", IN VITRO CELL. DEV. BIOL. PLANT, vol. 37, 2001, pages 427 - 433 *
RAMIREZ ET AL.: "Fruit-specific expression of the human immunodeficiency virus type 1 Tat Gene in tomato plants and its immunogenic potential in mice", CLINICAL AND VACCINE IMMUNOLOGY, vol. 14, no. 6, 2007, pages 685 - 692 *
ZHU-MEI HE ET AL.: "Assessment of the utility of the tomato fruit-specific E8 promoter for driving vaccine antigen expression", GENETICA, vol. 133, 2007, pages 207 - 214 *

Also Published As

Publication number Publication date
KR101101774B1 (ko) 2012-01-05
KR20100092289A (ko) 2010-08-20
CN102317456B (zh) 2013-05-08
CN102317456A (zh) 2012-01-11
WO2010093174A2 (ko) 2010-08-19
US8729340B2 (en) 2014-05-20
US20110321188A1 (en) 2011-12-29

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