WO2010047120A1 - PYRIDINE DERIVATIVE HAVING SUBSTITUTED HETERO RING AND SUBSTITUTED γ-GLUTAMYLAMINO GROUP, AND ANTI-FUNGAL AGENT COMPRISING SAME - Google Patents

PYRIDINE DERIVATIVE HAVING SUBSTITUTED HETERO RING AND SUBSTITUTED γ-GLUTAMYLAMINO GROUP, AND ANTI-FUNGAL AGENT COMPRISING SAME Download PDF

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WO2010047120A1
WO2010047120A1 PCT/JP2009/005559 JP2009005559W WO2010047120A1 WO 2010047120 A1 WO2010047120 A1 WO 2010047120A1 JP 2009005559 W JP2009005559 W JP 2009005559W WO 2010047120 A1 WO2010047120 A1 WO 2010047120A1
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group
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PCT/JP2009/005559
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田中圭悟
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エーザイ・アール・アンド・ディー・マネジメント株式会社
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Priority to CA2740982A priority Critical patent/CA2740982A1/en
Priority to RU2011116160/04A priority patent/RU2011116160A/en
Priority to JP2010534715A priority patent/JPWO2010047120A1/en
Priority to EP09821820A priority patent/EP2351752A4/en
Priority to NZ592416A priority patent/NZ592416A/en
Priority to BRPI0920614A priority patent/BRPI0920614A2/en
Application filed by エーザイ・アール・アンド・ディー・マネジメント株式会社 filed Critical エーザイ・アール・アンド・ディー・マネジメント株式会社
Priority to MX2011003389A priority patent/MX2011003389A/en
Priority to CN2009801414232A priority patent/CN102186846A/en
Priority to AU2009307574A priority patent/AU2009307574A1/en
Publication of WO2010047120A1 publication Critical patent/WO2010047120A1/en
Priority to IL211980A priority patent/IL211980A0/en
Priority to ZA2011/02840A priority patent/ZA201102840B/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D413/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms
    • C07D413/14—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms containing three or more hetero rings
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
    • A61K31/4427—Non condensed pyridines; Hydrogenated derivatives thereof containing further heterocyclic ring systems
    • A61K31/444—Non condensed pyridines; Hydrogenated derivatives thereof containing further heterocyclic ring systems containing a six-membered ring with nitrogen as a ring heteroatom, e.g. amrinone
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/10—Antimycotics
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D413/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms
    • C07D413/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms containing two hetero rings
    • C07D413/12—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms containing two hetero rings linked by a chain containing hetero atoms as chain links

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  • R 1 is a hydrogen atom, halogen atom, amino group, C 1-6 alkyl group, C 1-6 alkoxy group, C 1-6 alkylamino group, hydroxy C 1-6 alkylamino group or C 1-6 alkoxy C 1-6 alkyl group means a hydrogen atom, an amino group, or a C 1-6 alkoxy C 1-6 alkyl group, and the C 1-6 alkoxy C 1-6 alkyl group is preferably a methoxymethyl group Is preferred.
  • R is preferably a hydrogen atom, a methyl group, an ethyl group, or a 2-dimethylaminoethyl group.
  • Z represents a single bond, a methylene group, an ethylene group, an oxygen atom, a sulfur atom, -CH 2 O -, - OCH 2 -, - NH -, - NHCH 2 -, - CH 2 NH -, - CH 2 S-, Or —SCH 2 —, among which a methylene group, an oxygen atom, —CH 2 O—, or —OCH 2 — is preferable, and an oxygen atom, —CH 2 O—, or —OCH 2 — is particularly preferable.
  • the compounds according to the present invention are adjusted to pH adjusters, solubilizers, tonicity agents, etc., and if necessary, solubilizers, stabilizers, etc. And is formulated by a conventional method.
  • the method for producing the external preparation is not limited and can be produced by a conventional method. That is, as a base material used for formulation, various raw materials usually used for pharmaceuticals, quasi drugs, cosmetics, and the like can be used. Specific examples of the base material to be used include animal and vegetable oils, mineral oils, ester oils, waxes, higher alcohols, fatty acids, silicone oils, surfactants, phospholipids, alcohols, and polyhydric alcohols.
  • the form is not particularly limited, and it may be administered orally or parenterally by a commonly used method.
  • tablets, powders, granules, capsules, syrups, troches, inhalants, suppositories, injections, ointments, eye ointments, tapes, eye drops, nasal drops, ear drops, poultices It can be formulated and administered as an agent such as a lotion.
  • the suspension was filtered using a filter and washed with a mixed solution of n-heptane / ethanol (n-heptane (70 kg) / ethanol (10 kg)) and then n-heptane (80 kg). After drying with nitrogen for 15 minutes or more, the wet solid was taken out into a SUS container. The wet solid was dried under reduced pressure in a shelf dryer under hot water circulation at 45 to 50 ° C. to obtain the title compound (54.55 kg, yield: 58.6%).

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Abstract

Disclosed is an anti-fungal agent which has an excellent anti-fungal activity and excellent physical properties, particularly excellent solubility in water and excellent safety performance.  Specifically disclosed is a compound represented by formula (I) or a salt thereof.  [In the formula, R1 represents a hydrogen atom, a halogen atom, an amino group, R11-NH- (wherein R11 represents a C1-6 alkyl group, a hydroxy-C1-6-alkyl group, a C1-6-alkoxy-C1-6-alkyl group, or a C1-6 alkoxycarbonyl-C1-6-alkyl group), R12-(CO)-NH- [wherein R12 represents a C1-6 alkyl group or a C1-6-alkoxy-C1-6-alkyl group], a C1-6 alkyl group, a hydroxy-C1-6-alkyl group, a cyano-C1-6-alkyl group, a C1-6 alkoxy group, or a C1-6-alkoxy-C1-6-alkyl group; R2 represents a group represented by formula (II); one of X and Y represents a nitrogen atom and the other represents a nitrogen atom or an oxygen atom; the ring A represents a 5- or 6-membered heteroaryl or benzene ring which may have one or two halogen atoms or C1-6 alkyl groups; Z represents a single bond, a methylene group, an ethylene group, an oxygen atom, a sulfur atom, -CH2O-, -OCH2-, -NH-, -CH2NH-, -NHCH2-, -CH2S-, or -SCH2-; R3 represents a hydrogen atom, a halogen atom, or a C1-6 alkyl, C3-8 cycloalkyl, C6-10 aryl, 5- or 6-membered heteroaryl, or 5- or 6-membered non-aromatic heterocyclic group which may have one or two substituents independently selected from the substituent group α shown below; R4 represents a hydrogen atom, or a halogen atom; and R represents a hydrogen atom, or a C1-6 alkyl group which may be substituted by a dimethylamino group.] [Substituent group α] A halogen atom, a cyano group, a C1-6 alkyl group, a C1-6 alkoxy group, a C1-6 alkoxycarbonyl group, a C3-8 cycloalkyl group, a C2-6 alkenyl group, and a C2-6 alkynyl group.]

Description

ヘテロ環及びγ－グルタミルアミノ基が置換したピリジン誘導体並びにそれらを含有する抗真菌剤Pyridine derivatives substituted with heterocyclic and γ-glutamylamino groups and antifungal agents containing them
　本発明は、新規なヘテロ環及びγ－グルタミルアミノ基が置換したピリジン誘導体並びにそれらを含有する抗真菌剤に関する。 The present invention relates to a novel heterocyclic ring and a pyridine derivative substituted with a γ-glutamylamino group, and an antifungal agent containing them.
　近年、高度な化学療法等による免疫機能の低下した患者や高齢者が増加しているため、日和見感染の対策は益々重要性を増してきている。異なる弱毒菌による日和見感染が次々と起こっている事実が示すように、患者の抵抗力が低下するような基礎疾患がある限り感染症の問題は後を絶たない。従って、近い将来確実に訪れる高齢化社会においては、耐性菌の問題を含めた新たな感染症対策が重要な課題の一つとなることが見込まれている。 In recent years, as the number of patients and elderly people whose immune functions have declined due to advanced chemotherapy has increased, countermeasures against opportunistic infections have become increasingly important. As the fact that opportunistic infections with different attenuated bacteria occur one after another, as long as there are underlying diseases that reduce the patient's resistance, the problem of infectious diseases is constant. Therefore, in the aging society that will surely come in the near future, new countermeasures against infectious diseases including the problem of resistant bacteria are expected to be one of the important issues.
　抗真菌剤の分野では、従来、例えば、深在性の真菌症の治療にはポリエン系のアムホテリシンＢやアゾール系のフルコナゾール、イトラコナゾール、ボリコナゾール等が開発されてきた。すでに上市されている既存薬には類似したメカニズムの薬剤が多く、現在ではアゾール耐性菌等の出現が問題となっている。 In the field of antifungal agents, for example, polyene-based amphotericin B, azole-based fluconazole, itraconazole, voriconazole and the like have been developed for the treatment of deep mycosis. Many existing drugs already on the market have similar mechanisms, and the emergence of azole-resistant bacteria has become a problem at present.
　近年、新規メカニズムの１，３－β－グルカン合成酵素阻害剤として天然物由来の環状ヘキサペプチド型のカスポファンジンやミカファンジン等が開発されてきているが、これらの薬剤には注射剤しかないことから、抗真菌剤としてはまだ充分ではない。 In recent years, cyclic hexapeptide caspofandine and micafungin derived from natural products have been developed as 1,3-β-glucan synthase inhibitors with a novel mechanism. However, it is still not enough as an antifungal agent.
　このように既存の抗真菌剤では充分とはいえない状況にあり、新規なメカニズムに基づく安全性の高い薬剤の開発が切望されている。かかる新規なメカニズムに基づく抗真菌剤に関する関連技術として、特許文献１及び２がある。特許文献１及び２には、ＧＰＩ（glycosylphosphatidyl-inositol）アンカー蛋白質の細胞壁への輸送過程を阻害することで細胞壁表層蛋白質の発現を阻害し、細胞壁assemblyを阻害するとともに真菌が細胞へ付着するのを阻害して、病原体が病原性を発揮できないようにすることにより、感染症の発症、進展、持続に対して効果を示すピリジン誘導体が記載されている。 As described above, the existing antifungal agents are not sufficient, and the development of highly safe drugs based on a novel mechanism is eagerly desired. Patent Documents 1 and 2 are related technologies related to antifungal agents based on such a novel mechanism. Patent Documents 1 and 2 disclose that GPI (glycosylphosphatidyl-inositol) anchor protein transport process to the cell wall is inhibited to inhibit cell wall surface protein expression, cell wall assembly is inhibited, and fungi adhere to cells. Pyridine derivatives have been described that have an effect on the onset, progression and persistence of infectious diseases by inhibiting and preventing pathogens from exhibiting pathogenicity.
　このような状況下において、特許文献３には、従来の抗真菌剤にはない優れた抗真菌作用を有し、物性、安全性及び代謝的安定性の面でも優れた抗真菌剤として、ヘテロ環置換ピリジン誘導体が提案されている。 Under such circumstances, Patent Document 3 discloses a heterogeneous antifungal agent that has an excellent antifungal action that is not found in conventional antifungal agents, and that is also excellent in terms of physical properties, safety, and metabolic stability. Ring substituted pyridine derivatives have been proposed.
　一方、非特許文献１には、γ－グルタミル基を導入したプロドラッグとして、下記式で表わされる化合物が開示されている。 On the other hand, Non-Patent Document 1 discloses a compound represented by the following formula as a prodrug having a γ-glutamyl group introduced.
Figure JPOXMLDOC01-appb-C000001
Figure JPOXMLDOC01-appb-C000001
国際公開第０２／０４６２６号パンフレットInternational Publication No. 02/04626 Pamphlet 国際公開第０５／０３３０７９号パンフレットInternational Publication No. 05/033079 Pamphlet 国際公開第０７／０５２６１５号パンフレットInternational Publication No. 07/052615 Pamphlet
　しかしながら、現在までに、ＧＰＩアンカー蛋白質輸送過程の阻害に基づく優れた抗真菌作用を有し、かつ、水への溶解性及び水溶液中での安定性、並びに体内動態及び安全性の面でも優れる水溶性プロドラッグは知られておらず、注射剤として実用的なＧＰＩアンカー蛋白質輸送過程の阻害に基づく優れた抗真菌剤が望まれている。 However, to date, it has excellent antifungal activity based on inhibition of the GPI anchor protein transport process, and is also excellent in water solubility and stability in aqueous solution, as well as in pharmacokinetics and safety. No sex prodrug is known, and an excellent antifungal agent based on inhibition of the GPI-anchored protein transport process practical as an injection is desired.
　かかる事情に鑑み、本発明の目的は、ＧＰＩアンカー蛋白質輸送過程の阻害に基づく優れた抗真菌作用を有し、かつ、水への溶解性及び水溶液中での安定性、並びに体内動態及び安全性の面でも優れる抗真菌剤を提供することにある。 In view of such circumstances, the object of the present invention is to have an excellent antifungal action based on inhibition of the GPI anchor protein transport process, and to be soluble in water and stable in aqueous solution, as well as pharmacokinetics and safety. It is in providing the antifungal agent which is excellent also in the aspect.
　本発明者らは、上記事情に鑑み鋭意研究を重ねた結果、 The inventors have conducted extensive research in view of the above circumstances,
Figure JPOXMLDOC01-appb-C000002
Figure JPOXMLDOC01-appb-C000002
で表される、ヘテロ環及びγ－グルタミルアミノ基が置換したピリジン誘導体が、優れた抗真菌作用を有するとともに、水への溶解性及び水溶液中での安定性、並びに体内動態及び安全性の面でも優れることをも見出して、本発明を完成した。 A pyridine derivative substituted with a heterocyclic ring and a γ-glutamylamino group represented by the formula has excellent antifungal activity, solubility in water, stability in aqueous solution, and pharmacokinetics and safety. However, the present invention was completed by finding out that it is excellent.
　すなわち、本発明は、
［１］下式（I）で表される化合物又はその塩；
Figure JPOXMLDOC01-appb-C000003
 　式中、
　Ｒ1が、水素原子、ハロゲン原子、アミノ基、Ｒ11－ＮＨ－（Ｒ11が、Ｃ1-6アルキル基、ヒドロキシＣ1-6アルキル基、Ｃ1-6アルコキシＣ1-6アルキル基、又はＣ1-6アルコキシカルボニルＣ1-6アルキル基を意味する。）、Ｒ12－（ＣＯ）－ＮＨ－（Ｒ12が、Ｃ1-6アルキル基又はＣ1-6アルコキシＣ1-6アルキル基）、Ｃ1-6アルキル基、ヒドロキシＣ1-6アルキル基、シアノＣ1-6アルキル基、Ｃ1-6アルコキシ基、又はＣ1-6アルコキシＣ1-6アルキル基を意味し；
　Ｒ2が式
Figure JPOXMLDOC01-appb-C000004
 で表される基を意味し；
　Ｘ及びＹの一方が、窒素原子を、他方が、窒素原子又は酸素原子を意味し；
　環Ａが、ハロゲン原子若しくはＣ1-6アルキル基を１個若しくは２個有していてもよい、５若しくは６員のへテロアリール環又はベンゼン環を意味し；
　Ｚが、単結合、メチレン基、エチレン基、酸素原子、硫黄原子、－ＣＨ2Ｏ－、－ＯＣＨ2－、－ＮＨ－、－ＣＨ2ＮＨ－、－ＮＨＣＨ2－、－ＣＨ2Ｓ－、又は－ＳＣＨ2－を意味し；
　Ｒ3が、水素原子、ハロゲン原子、又は、それぞれ置換基群αから選ばれる置換基を１個若しくは２個有していてもよい、Ｃ1-6アルキル基、Ｃ3-8シクロアルキル基、Ｃ6-10アリール基、５若しくは６員へテロアリール基、又は５若しくは６員の非芳香族系へテロ環式基を意味し；
　Ｒ4が、水素原子又はハロゲン原子を意味し；
　Ｒが、水素原子、又はジメチルアミノ基で置換されていてもよいＣ1-6アルキル基を意味する。
［置換基群α］
ハロゲン原子、シアノ基、Ｃ1-6アルキル基、Ｃ1-6アルコキシ基、Ｃ1-6アルコキシカルボニル基、Ｃ3-8シクロアルキル基、Ｃ2-6アルケニル基、及びＣ2-6アルキニル基。
［２］
Figure JPOXMLDOC01-appb-C000005
 で表される部分構造が、下記の群から選ばれる部分構造である前項［１］に記載の化合物又はその塩。
Figure JPOXMLDOC01-appb-C000006
 ［３］　Ｘ及びＹの一方が窒素原子で、他方が酸素原子である前項［１］に記載の化合物又はその塩。
［４］
Figure JPOXMLDOC01-appb-C000007
 で表される部分構造が、下式（ＩＩＩ）
Figure JPOXMLDOC01-appb-C000008
 で表される部分構造、又は下式（ＩＶ）
Figure JPOXMLDOC01-appb-C000009
 で表される部分構造である前項［３］に記載の化合物又はその塩。
［５］　Ｘ及びＹがともに窒素原子である前項［１］に記載の化合物又はその塩。
［６］
Figure JPOXMLDOC01-appb-C000010
 で表される部分構造が、下式（Ｖ）
Figure JPOXMLDOC01-appb-C000011
 で表される部分構造又は下式（ＶＩ）
Figure JPOXMLDOC01-appb-C000012
 で表される部分構造である前項［５］に記載の化合物又はその塩。
［７］　Ｒが水素原子、メチル基、エチル基、又は２－ジメチルアミノエチル基である前項［１］ないし［６］のいずれか１項に記載の化合物又はその塩。
［８］　Ｒ1が、水素原子、アミノ基、又はＣ1-6アルコキシＣ1-6アルキル基である前項［７］に記載の化合物又はその塩。
［９］　Ｒ1がアミノ基であって、Ｒが水素原子、メチル基、エチル基、又は２－ジメチルアミノエチル基である前項［１］ないし［６］のいずれか１項に記載の化合物又はその塩。
［１０］Ｒ1がアミノ基であって、Ｒがメチル基、エチル基、又は２－ジメチルアミノエチル基である前項［１］ないし［６］のいずれか１項に記載の化合物又はその塩。
［１１］　環Ａが、ピリジン環、ベンゼン環、フラン環、チオフェン環、又はピロール環である前項［１］ないし［１０］のいずれか１項に記載の化合物又はその塩。
［１２］　環Ａが、ピリジン環又はベンゼン環である前項［１１］に記載の化合物又はその塩。
［１３］　Ｚが、酸素原子、－ＣＨ2Ｏ－、又は－ＯＣＨ2－である前項［１］ないし［１２］のいずれか１項に記載の化合物又はその塩。
［１４］　前項［１］ないし［１３］のいずれか１項に記載の化合物又はその塩を含有する医薬組成物。
［１５］　前項［１］ないし［１３］のいずれか１項に記載の化合物又はその塩を含有する医薬。
［１６］　前項［１］ないし［１３］のいずれか１項に記載の化合物又はその塩を有効成分とする抗真菌剤。
［１７］　前項［１］ないし［１３］のいずれか１項に記載の化合物又はその塩の薬理学的有効量を投与して、真菌感染症を予防及び／又は治療する方法。
［１８］　抗真菌剤の製造のための前項［１］ないし［１３］のいずれか１項に記載の化合物又はその塩の使用。
を提供する。 That is, the present invention
[1] A compound represented by the following formula (I) or a salt thereof;
Figure JPOXMLDOC01-appb-C000003
 Where
R 1 is a hydrogen atom, a halogen atom, an amino group, R 11 —NH— (R 11 is a C 1-6 alkyl group, a hydroxy C 1-6 alkyl group, a C 1-6 alkoxy C 1-6 alkyl group, Or a C 1-6 alkoxycarbonyl C 1-6 alkyl group), R 12 — (CO) —NH— (wherein R 12 represents a C 1-6 alkyl group or a C 1-6 alkoxy C 1-6 alkyl group). Group), a C 1-6 alkyl group, a hydroxy C 1-6 alkyl group, a cyano C 1-6 alkyl group, a C 1-6 alkoxy group, or a C 1-6 alkoxy C 1-6 alkyl group;
R 2 is the formula
Figure JPOXMLDOC01-appb-C000004
 Means a group represented by:
One of X and Y means a nitrogen atom, and the other means a nitrogen atom or an oxygen atom;
Ring A represents a 5- or 6-membered heteroaryl ring or benzene ring optionally having one or two halogen atoms or C 1-6 alkyl groups;
Z is a single bond, a methylene group, an ethylene group, an oxygen atom, a sulfur atom, -CH 2 O -, - OCH 2 -, - NH -, - CH 2 NH -, - NHCH 2 -, - CH 2 S-, Or -SCH 2- ;
R 3 may have a hydrogen atom, a halogen atom, or one or two substituents each selected from substituent group α, a C 1-6 alkyl group, a C 3-8 cycloalkyl group, Means a C 6-10 aryl group, a 5 or 6 membered heteroaryl group, or a 5 or 6 membered non-aromatic heterocyclic group;
R 4 represents a hydrogen atom or a halogen atom;
R represents a hydrogen atom or a C 1-6 alkyl group which may be substituted with a dimethylamino group.
[Substituent group α]
Halogen atom, cyano group, C 1-6 alkyl group, C 1-6 alkoxy group, C 1-6 alkoxycarbonyl group, C 3-8 cycloalkyl group, C 2-6 alkenyl group, and C 2-6 alkynyl group .
[2]
Figure JPOXMLDOC01-appb-C000005
 The compound or a salt thereof according to item [1], wherein the partial structure represented by the formula is a partial structure selected from the following group.
Figure JPOXMLDOC01-appb-C000006
 [3] The compound or a salt thereof according to [1], wherein one of X and Y is a nitrogen atom and the other is an oxygen atom.
[4]
Figure JPOXMLDOC01-appb-C000007
 The partial structure represented by the following formula (III)
Figure JPOXMLDOC01-appb-C000008
 Or a partial structure represented by the following formula (IV)
Figure JPOXMLDOC01-appb-C000009
 The compound or a salt thereof according to [3] above, which is a partial structure represented by
[5] The compound or salt thereof according to [1], wherein X and Y are both nitrogen atoms.
[6]
Figure JPOXMLDOC01-appb-C000010
 The partial structure represented by the following formula (V)
Figure JPOXMLDOC01-appb-C000011
 Or a partial structure represented by the following formula (VI)
Figure JPOXMLDOC01-appb-C000012
 The compound or a salt thereof according to the above item [5], which is a partial structure represented by the formula:
[7] The compound or a salt thereof according to any one of [1] to [6], wherein R is a hydrogen atom, a methyl group, an ethyl group, or a 2-dimethylaminoethyl group.
[8] The compound or salt thereof according to [7] above, wherein R 1 is a hydrogen atom, an amino group, or a C 1-6 alkoxy C 1-6 alkyl group.
[9] The compound according to any one of [1] to [6], wherein R 1 is an amino group, and R is a hydrogen atom, a methyl group, an ethyl group, or a 2-dimethylaminoethyl group; Its salt.
[10] The compound or salt thereof according to any one of [1] to [6], wherein R 1 is an amino group, and R is a methyl group, an ethyl group, or a 2-dimethylaminoethyl group.
[11] The compound or salt thereof according to any one of [1] to [10], wherein Ring A is a pyridine ring, a benzene ring, a furan ring, a thiophene ring, or a pyrrole ring.
[12] The compound or salt thereof according to [11], wherein ring A is a pyridine ring or a benzene ring.
[13] The compound or the salt thereof according to any one of [1] to [12], wherein Z is an oxygen atom, —CH 2 O—, or —OCH 2 —.
[14] A pharmaceutical composition comprising the compound or salt thereof according to any one of [1] to [13].
[15] A medicament comprising the compound or salt thereof according to any one of [1] to [13].
[16] An antifungal agent comprising the compound according to any one of [1] to [13] or a salt thereof as an active ingredient.
[17] A method for preventing and / or treating a fungal infection by administering a pharmacologically effective amount of the compound or salt thereof according to any one of [1] to [13].
[18] Use of the compound according to any one of [1] to [13] or a salt thereof for the manufacture of an antifungal agent.
I will provide a.
　式（Ｉ）で表される化合物又はその塩（以下、単に「本発明化合物」という称する場合がある。）は、１）真菌のＧＰＩ生合成阻害に基づいて細胞壁表層蛋白質の発現を阻害し、細胞壁assemblyを阻害するとともに真菌が細胞へ付着するのを阻害して、病原体が病原性を発揮できないようにすることにより、感染症の発症、進展、持続に対して効果を示し、さらに、２）物性、特に、水への溶解性及び水溶液中での安定性、並びに体内動態及び安全性の面でも優れる真菌感染症の予防又は治療剤として極めて有用である。 The compound represented by the formula (I) or a salt thereof (hereinafter sometimes simply referred to as “the compound of the present invention”) 1) inhibits the expression of cell wall surface protein based on inhibition of fungal GPI biosynthesis, Inhibiting cell wall assembly and preventing fungi from attaching to cells to prevent pathogens from demonstrating pathogenicity, and is effective in the onset, progression and persistence of infectious diseases, and 2) It is extremely useful as a preventive or therapeutic agent for fungal infections that are excellent in physical properties, in particular, solubility in water and stability in aqueous solutions, as well as in pharmacokinetics and safety.
本発明の一の実施態様にて、マウスにおける薬物動態評価法において測定された実施例１の化合物及び親化合物である活性体の血漿中濃度を測定した結果を示す図である。In one embodiment of this invention, it is a figure which shows the result of having measured the plasma density | concentration of the active substance which is the compound of Example 1 and the parent compound which were measured in the pharmacokinetic evaluation method in a mouse | mouth. 本発明の別の実施態様にて、マウスにおける薬物動態評価法において測定された実施例２、３の化合物及び親化合物である活性体の血漿中濃度を測定した結果を示す図である。It is a figure which shows the result of having measured the plasma density | concentration of the active body which is the compound of Example 2, 3 and the parent compound which were measured in the pharmacokinetic evaluation method in a mouse | mouth in another embodiment of this invention.
　以下に、本明細書において記載する記号、用語等の定義、本発明の実施の形態等を示して、本発明を詳細に説明する。なお、本発明は以下の実施の形態に限定されるものではなく、その要旨の範囲内で種々変形して実施することができる。 Hereinafter, the present invention will be described in detail by showing definitions of symbols, terms and the like described in the present specification, embodiments of the present invention, and the like. In addition, this invention is not limited to the following embodiment, It can implement by changing variously within the range of the summary.
　本明細書中においては、化合物の構造式が便宜上一定の異性体を表すことがあるが、本発明には化合物の構造上生じ得るすべての幾何異性体、不斉炭素に基づく光学異性体、立体異性体、回転異性体、互変異性体等の異性体及び異性体混合物を含み、便宜上の式の記載に限定されるものではなく、いずれか一方の異性体でも混合物でもよい。したがって、本発明の化合物には、分子内に不斉炭素原子を有し光学活性体及びラセミ体が存在することがありうるが、本発明においては限定されず、いずれもが含まれる。また、結晶多形が存在することもあるが同様に限定されず、いずれかの単一の結晶形であっても二以上の結晶形からなる混合物であってもよい。そして、本発明化合物には無水物と水和物等の溶媒和物とが包含される。 In the present specification, the structural formula of a compound may represent a certain isomer for convenience, but the present invention includes all geometrical isomers that can occur in the structure of the compound, optical isomers based on asymmetric carbon, stereo It includes isomers such as isomers, rotational isomers, tautomers, and isomer mixtures, and is not limited to the description of formulas for convenience, and may be either one isomer or a mixture. Therefore, the compound of the present invention may have an asymmetric carbon atom in the molecule, and an optically active substance and a racemate may exist. However, the present invention is not limited and includes both. In addition, there may be a crystal polymorph, but it is not limited in the same manner, and any single crystal form or a mixture of two or more crystal forms may be used. The compounds of the present invention include anhydrides and solvates such as hydrates.
　本明細書において使用する「Ｃ1-6アルキル基」とは、炭素数１～６個の脂肪族炭化水素から任意の水素原子を１個除いて誘導される一価の基である、炭素数１～６個の直鎖状又は分枝鎖状のアルキル基を意味し、具体的には例えば、メチル基、エチル基、ｎ－プロピル基、イソプロピル基、ｎ－ブチル基、イソブチル基、ｓｅｃ－ブチル基、ｔｅｒｔ－ブチル基、ｎ－ペンチル基、イソペンチル基、ｓｅｃ－ペンチル基、ネオペンチル基、１－メチルブチル基、２－メチルブチル基、１，１－ジメチルプロピル基、１，２－ジメチルプロピル基、ｎ－ヘキシル基、イソヘキシル基、１－メチルペンチル基、２－メチルペンチル基、３－メチルペンチル基、１，１－ジメチルブチル基、１，２－ジメチルブチル基、２，２－ジメチルブチル基、１，３－ジメチルブチル基、２，３－ジメチルブチル基、３，３－ジメチルブチル基、１－エチルブチル基、２－エチルブチル基、１，１，２－トリメチルプロピル基、１，２，２－トリメチルプロピル基、１－エチル－１－メチルプロピル基、１－エチル－２－メチルプロピル基等が挙げられ、好ましくはメチル基、エチル基、ｎ－プロピル基、イソプロピル基、ｎ－ブチル基、イソブチル基、ｓｅｃ－ブチル基、ｔｅｒｔ－ブチル基等である。 The “C 1-6 alkyl group” used in the present specification is a monovalent group derived by removing any one hydrogen atom from an aliphatic hydrocarbon having 1 to 6 carbon atoms. 1 to 6 linear or branched alkyl groups, specifically, for example, methyl group, ethyl group, n-propyl group, isopropyl group, n-butyl group, isobutyl group, sec- Butyl group, tert-butyl group, n-pentyl group, isopentyl group, sec-pentyl group, neopentyl group, 1-methylbutyl group, 2-methylbutyl group, 1,1-dimethylpropyl group, 1,2-dimethylpropyl group, n-hexyl group, isohexyl group, 1-methylpentyl group, 2-methylpentyl group, 3-methylpentyl group, 1,1-dimethylbutyl group, 1,2-dimethylbutyl group, 2,2-dimethylbutyl group, 1,3-dimethylbutyl group, 2,3-dimethylbutyl group, 3,3-dimethylbutyl group, 1-ethylbutyl group, 2-ethylbutyl group, 1,1,2-trimethylpropyl group, 1,2,2- Examples include trimethylpropyl group, 1-ethyl-1-methylpropyl group, 1-ethyl-2-methylpropyl group, and the like, preferably methyl group, ethyl group, n-propyl group, isopropyl group, n-butyl group, isobutyl Group, sec-butyl group, tert-butyl group and the like.
　本明細書において使用する「Ｃ2-6アルケニル基」とは、二重結合を１～２個含んでいてもよい炭素数２～６個の直鎖状又は分枝鎖状のアルケニル基を意味し、具体的には例えば、エテニル基、１－プロペニル基、２－プロペニル基、１－ブテニル基、２－ブテニル基、３－ブテニル基、２－メチル－１－プロペニル基、ペンテニル基、３－メチル－２－ブテニル基、ヘキセニル基、ヘキサンジエニル基等が挙げられ、好ましくはエテニル基、１－プロペニル基、２－プロペニル基、１－ブテニル基、２－ブテニル基、３－ブテニル基、２－メチル－１－プロペニル基、３－メチル－２－ブテニル基等である。 As used herein, “C 2-6 alkenyl group” means a linear or branched alkenyl group having 2 to 6 carbon atoms which may contain 1 to 2 double bonds. Specifically, for example, ethenyl group, 1-propenyl group, 2-propenyl group, 1-butenyl group, 2-butenyl group, 3-butenyl group, 2-methyl-1-propenyl group, pentenyl group, 3- Examples thereof include a methyl-2-butenyl group, a hexenyl group, a hexanedienyl group, and the like, and preferably an ethenyl group, a 1-propenyl group, a 2-propenyl group, a 1-butenyl group, a 2-butenyl group, a 3-butenyl group, 2 -Methyl-1-propenyl group, 3-methyl-2-butenyl group and the like.
　本明細書において使用する「Ｃ2-6アルキニル基」とは、三重結合を１～２個含んでいてもよい炭素数２～６個の直鎖状又は分枝鎖状のアルキニル基を意味し、具体的には例えば、エチニル基、１－プロピニル基、２－プロピニル基、１－ブチニル基、２－ブチニル基、３－ブチニル基、ペンチニル基、ヘキシニル基、ヘキサンジイニル基等が挙げられ、好ましくはエチニル基、１－プロピニル基、２－プロピニル基、１－ブチニル基、２－ブチニル基、３－ブチニル基等である。 As used herein, “C 2-6 alkynyl group” means a linear or branched alkynyl group having 2 to 6 carbon atoms which may contain 1 to 2 triple bonds. Specific examples include ethynyl group, 1-propynyl group, 2-propynyl group, 1-butynyl group, 2-butynyl group, 3-butynyl group, pentynyl group, hexynyl group, hexanediynyl group and the like. Preferred are ethynyl group, 1-propynyl group, 2-propynyl group, 1-butynyl group, 2-butynyl group, 3-butynyl group and the like.
　本明細書において使用する「Ｃ3-8シクロアルキル基」とは、炭素数３～８個の環状の脂肪族炭化水素基を意味し、具体的には例えば、シクロプロピル基、シクロブチル基、シクロペンチル基、シクロヘキシル基、シクロヘプチル基、シクロオクチル基等が挙げられ、好ましくはシクロプロピル基、シクロブチル基、シクロペンチル基、シクロヘキシル基等である。 As used herein, “C 3-8 cycloalkyl group” means a cyclic aliphatic hydrocarbon group having 3 to 8 carbon atoms, and specifically includes, for example, a cyclopropyl group, a cyclobutyl group, a cyclopentyl group. Group, cyclohexyl group, cycloheptyl group, cyclooctyl group and the like, and preferred are cyclopropyl group, cyclobutyl group, cyclopentyl group, cyclohexyl group and the like.
　本明細書において使用する「Ｃ1-6アルコキシ基」とは、前記定義「Ｃ1-6アルキル基」の末端に酸素原子が結合した基であることを意味し、具体的には例えば、メトキシ基、エトキシ基、ｎ－プロポキシ基、イソプロポキシ基、ｎ－ブトキシ基、イソブトキシ基、ｓｅｃ－ブトキシ基、ｔｅｒｔ－ブトキシ基、ｎ－ペンチルオキシ基、イソペンチルオキシ基、ｓｅｃ－ペンチルオキシ基、ネオペンチルオキシ基、１－メチルブトキシ基、２－メチルブトキシ基、１，１－ジメチルプロポキシ基、１，２－ジメチルプロポキシ基、ｎ－ヘキシルオキシ基、イソヘキシルオキシ基、１－メチルペンチルオキシ基、２－メチルペンチルオキシ基、３－メチルペンチルオキシ基、１，１－ジメチルブトキシ基、１，２－ジメチルブトキシ基、２，２－ジメチルブトキシ基、１，３－ジメチルブトキシ基、２，３－ジメチルブトキシ基、３，３－ジメチルブトキシ基、１－エチルブトキシ基、２－エチルブトキシ基、１，１，２－トリメチルプロポキシ基、１，２，２－トリメチルプロポキシ基、１－エチル－１－メチルプロポキシ基、１－エチル－２－メチルプロポキシ基等が挙げられ、好ましくはメトキシ基、エトキシ基、ｎ－プロポキシ基、イソプロポキシ基、ｎ－ブトキシ基、イソブトキシ基、ｓｅｃ－ブトキシ基、ｔｅｒｔ－ブトキシ基等である。 As used herein, “C 1-6 alkoxy group” means a group in which an oxygen atom is bonded to the terminal of the above-mentioned definition “C 1-6 alkyl group”. Group, ethoxy group, n-propoxy group, isopropoxy group, n-butoxy group, isobutoxy group, sec-butoxy group, tert-butoxy group, n-pentyloxy group, isopentyloxy group, sec-pentyloxy group, neo Pentyloxy group, 1-methylbutoxy group, 2-methylbutoxy group, 1,1-dimethylpropoxy group, 1,2-dimethylpropoxy group, n-hexyloxy group, isohexyloxy group, 1-methylpentyloxy group, 2-methylpentyloxy group, 3-methylpentyloxy group, 1,1-dimethylbutoxy group, 1,2-dimethylbutoxy group, 2,2- Methylbutoxy group, 1,3-dimethylbutoxy group, 2,3-dimethylbutoxy group, 3,3-dimethylbutoxy group, 1-ethylbutoxy group, 2-ethylbutoxy group, 1,1,2-trimethylpropoxy group, 1,2,2-trimethylpropoxy group, 1-ethyl-1-methylpropoxy group, 1-ethyl-2-methylpropoxy group and the like are preferable, preferably methoxy group, ethoxy group, n-propoxy group, isopropoxy group N-butoxy group, isobutoxy group, sec-butoxy group, tert-butoxy group and the like.
　本明細書において使用する「ヒドロキシＣ1-6アルキル基」とは、前記定義「Ｃ1-6アルキル基」中の任意の水素原子を、水酸基で置換した基を意味し、具体的には、ヒドロキシメチル基、１－ヒドロキシエチル基、２－ヒドロキシエチル基、１－ヒドロキシ－ｎ－プロピル基、２－ヒドロキシ－ｎ－プロピル基、３－ヒドロキシ－ｎ－プロピル基、１－ヒドロキシ－イソプロピル基、２－ヒドロキシ－イソプロピル基、３－ヒドロキシ－イソプロピル基、１－ヒドロキシ－ｔｅｒｔ－ブチル基等が挙げられ、好ましくは、ヒドロキシメチル基、１－ヒドロキシエチル基、２－ヒドロキシエチル基等である。 As used herein, “hydroxy C 1-6 alkyl group” means a group obtained by substituting any hydrogen atom in the above-defined “C 1-6 alkyl group” with a hydroxyl group. Specifically, Hydroxymethyl group, 1-hydroxyethyl group, 2-hydroxyethyl group, 1-hydroxy-n-propyl group, 2-hydroxy-n-propyl group, 3-hydroxy-n-propyl group, 1-hydroxy-isopropyl group, Examples include 2-hydroxy-isopropyl group, 3-hydroxy-isopropyl group, 1-hydroxy-tert-butyl group, and the like, preferably hydroxymethyl group, 1-hydroxyethyl group, 2-hydroxyethyl group and the like.
　本明細書において使用する「シアノＣ1-6アルキル基」とは、前記定義「Ｃ1-6アルキル基」中の任意の水素原子を、シアノ基で置換した基を意味し、具体的には、シアノメチル基、１－シアノエチル基、２－シアノエチル基、１－シアノ－ｎ－プロピル基、２－シアノ－ｎ－プロピル基、３－シアノ－ｎ－プロピル基、１－シアノ－イソプロピル基、２－シアノ－イソプロピル基、３－シアノ－イソプロピル基、１－シアノ－ｔｅｒｔ－ブチル基等が挙げられ、好ましくは、シアノメチル基、１－シアノエチル基、２－シアノエチル基等である。 As used herein, “cyano C 1-6 alkyl group” means a group in which any hydrogen atom in the above-defined “C 1-6 alkyl group” is substituted with a cyano group. Cyanomethyl group, 1-cyanoethyl group, 2-cyanoethyl group, 1-cyano-n-propyl group, 2-cyano-n-propyl group, 3-cyano-n-propyl group, 1-cyano-isopropyl group, 2- A cyano-isopropyl group, a 3-cyano-isopropyl group, a 1-cyano-tert-butyl group and the like are mentioned, and a cyanomethyl group, a 1-cyanoethyl group, a 2-cyanoethyl group and the like are preferable.
　本明細書において使用する「Ｃ1-6アルコキシカルボニル基」とは、前記定義「Ｃ1-6アルコキシ基」の末端にカルボニル基が結合した基であることを意味し、具体的には例えば、メトキシカルボニル基、エトキシカルボニル基、ｎ－プロポキシカルボニル基、イソプロポキシカルボニル基等が挙げられる。 The "C 1-6 alkoxycarbonyl group" as used herein, means that the terminal carbonyl group of the defined "C 1-6 alkoxy group" is a group attached, specifically, for example, Examples include methoxycarbonyl group, ethoxycarbonyl group, n-propoxycarbonyl group, isopropoxycarbonyl group and the like.
　本明細書において使用する「Ｃ1-6アルコキシカルボニルＣ1-6アルキル基」とは、前記定義「Ｃ1-6アルコキシカルボニル基」の末端に、前記定義「Ｃ1-6アルキル基」が結合した基であることを意味し、具体的には例えば、メトキシカルボニルメチル基、メトキシカルボニルエチル基、エトキシカルボニルメチル基、エトキシカルボニルエチル基等が挙げられる。 The "C 1-6 alkoxycarbonyl C 1-6 alkyl group" as used herein, the the end of the definition "C 1-6 alkoxycarbonyl group", the definition "C 1-6 alkyl group" is bonded Specifically, examples thereof include a methoxycarbonylmethyl group, a methoxycarbonylethyl group, an ethoxycarbonylmethyl group, and an ethoxycarbonylethyl group.
　本明細書において使用する「Ｃ6-10アリール基」とは、炭素数６～１０の芳香族の炭化水素環式基をいい、具体的には例えば、フェニル基、１－ナフチル基、２－ナフチル基、インデニル基、アズレニル基、ヘプタレニル基等が挙げられ、好ましくはフェニル基、１－ナフチル基、２－ナフチル基等である。 As used herein, the “C 6-10 aryl group” refers to an aromatic hydrocarbon cyclic group having 6 to 10 carbon atoms, and specifically includes, for example, a phenyl group, a 1-naphthyl group, a 2- A naphthyl group, an indenyl group, an azulenyl group, a heptalenyl group and the like can be mentioned, and a phenyl group, a 1-naphthyl group, a 2-naphthyl group and the like are preferable.
　本明細書中において使用する「Ｃ1-6アルコキシＣ1-6アルキル基」とは、前記定義「Ｃ1-6アルキル基」中の任意の水素原子を、前記定義「Ｃ1-6アルコキシ基」で置換した基を意味し、具体的には例えば、メトキシメチル基、エトキシメチル基、ｎ－プロポキシメチル、メトキシエチル基、エトキシエチル基等が挙げられる。 The "C 1-6 alkoxy C 1-6 alkyl group" as used herein, any hydrogen atom in the definition "C 1-6 alkyl group", the definition "C 1-6 alkoxy group ], Specifically, for example, methoxymethyl group, ethoxymethyl group, n-propoxymethyl, methoxyethyl group, ethoxyethyl group and the like.
　本明細書において使用する「ハロゲン原子」とは、フッ素原子、塩素原子、臭素原子又はヨウ素原子を意味する。 As used herein, “halogen atom” means a fluorine atom, a chlorine atom, a bromine atom or an iodine atom.
　本明細書において使用する「ヘテロ原子」とは、窒素原子、硫黄原子又は酸素原子を意味する。 As used herein, “heteroatom” means a nitrogen atom, a sulfur atom or an oxygen atom.
　本明細書において使用する「５若しくは６員のヘテロアリール環」とは、環を構成する原子の数が５若しくは６であり、環を構成する原子中に１から複数個のヘテロ原子を含有する芳香族の環を意味する。具体的には例えば、フラン環、チオフェン環、ピロール環、ピリジン環、ピラジン環、ピリダジン環、ピリミジン環、トリアゾール環（１，２，３－トリアゾール環、１，２，４－トリアゾール環等）、テトラゾール環（例えば１Ｈ－テトラゾール環、２Ｈ－テトラゾール環等）、チアゾール環、ピラゾール環、オキサゾール環、イソオキサゾール環、イソチアゾール環、オキサジアゾール環、チアジアゾール環等が挙げられる。 As used herein, the term “5- or 6-membered heteroaryl ring” means that the number of atoms constituting the ring is 5 or 6, and one to a plurality of heteroatoms are contained in the atoms constituting the ring. An aromatic ring is meant. Specifically, for example, furan ring, thiophene ring, pyrrole ring, pyridine ring, pyrazine ring, pyridazine ring, pyrimidine ring, triazole ring (1,2,3-triazole ring, 1,2,4-triazole ring, etc.), Examples thereof include a tetrazole ring (for example, 1H-tetrazole ring, 2H-tetrazole ring, etc.), thiazole ring, pyrazole ring, oxazole ring, isoxazole ring, isothiazole ring, oxadiazole ring, thiadiazole ring and the like.
　本明細書において使用する「５若しくは６員ヘテロアリール基」とは、環を構成する原子の数が５若しくは６であり、環を構成する原子中に１から複数個のヘテロ原子を含有する芳香族の環から任意の位置の水素原子を１個除いて誘導される一価の基を意味する。具体的には例えば、フリル基（例えば２－フリル基、３－フリル基等）、チエニル基（例えば２－チエニル基、３－チエニル基等）、ピロリル基（例えば１－ピロリル基、２－ピロリル基、３－ピロリル基等）、ピリジル基（例えば２－ピリジル基、３－ピリジル基、４－ピリジル基等）、ピラジニル基、ピリダジニル基（例えば３－ピリダジニル基、４－ピリダジニル基等）、ピリミジニル基（例えば２－ピリミジニル基、４－ピリミジニル基、５－ピリミジニル基等）、トリアゾリル基（例えば１，２，３－トリアゾリル基、１，２，４－トリアゾリル基等）、テトラゾリル基（例えば１Ｈ－テトラゾリル基、２Ｈ－テトラゾリル基等）、チアゾリル基（例えば２－チアゾリル基、４－チアゾリル基、５－チアゾリル基等）、ピラゾリル基（例えば３－ピラゾリル基、４－ピラゾリル基等）、オキサゾリル基（例えば２－オキサゾリル基、４－オキサゾリル基、５－オキサゾリル基等）、イソオキサゾリル基（例えば３－イソオキサゾリル基、４－イソオキサゾリル基、５－イソオキサゾリル基等）、イソチアゾリル基（例えば３－イソチアゾリル基、４－イソチアゾリル基、５－イソチアゾリル基等）、オキサジアゾリル基、チアジアゾリル基等が挙げられる。 As used herein, the term “5- or 6-membered heteroaryl group” refers to an aromatic group in which the number of atoms constituting a ring is 5 or 6, and one to a plurality of heteroatoms are contained in the atoms constituting the ring. It means a monovalent group derived by removing one hydrogen atom at an arbitrary position from a group ring. Specifically, for example, furyl group (for example, 2-furyl group, 3-furyl group, etc.), thienyl group (for example, 2-thienyl group, 3-thienyl group, etc.), pyrrolyl group (for example, 1-pyrrolyl group, 2-pyrrolyl group, etc.) Group, 3-pyrrolyl group, etc.), pyridyl group (eg 2-pyridyl group, 3-pyridyl group, 4-pyridyl group etc.), pyrazinyl group, pyridazinyl group (eg 3-pyridazinyl group, 4-pyridazinyl group etc.), pyrimidinyl Groups (for example, 2-pyrimidinyl group, 4-pyrimidinyl group, 5-pyrimidinyl group, etc.), triazolyl groups (for example, 1,2,3-triazolyl group, 1,2,4-triazolyl group, etc.), tetrazolyl groups (for example, 1H- Tetrazolyl group, 2H-tetrazolyl group, etc.), thiazolyl group (eg 2-thiazolyl group, 4-thiazolyl group, 5-thiazolyl group, etc.), pyrazolyl (Eg 3-pyrazolyl group, 4-pyrazolyl group etc.), oxazolyl group (eg 2-oxazolyl group, 4-oxazolyl group, 5-oxazolyl group etc.), isoxazolyl group (eg 3-isoxazolyl group, 4-isoxazolyl group, 5 -Isoxazolyl group), isothiazolyl group (for example, 3-isothiazolyl group, 4-isothiazolyl group, 5-isothiazolyl group, etc.), oxadiazolyl group, thiadiazolyl group and the like.
　本明細書において使用する「５若しくは６員の非芳香族系へテロ環式基」とは、環を構成する原子の数が５若しくは６であり、環を構成する原子中に１から複数個のヘテロ原子を含有する非芳香族の環から任意の位置の水素原子を１個除いて誘導される一価の基を意味する。具体的には例えば、ピロリジニル基、ピペラジニル基、ピペリジニル基、モルホリニル基、テトラヒドロフリル基、テトラヒドロピラニル基等が挙げられる。 As used herein, the term “5- or 6-membered non-aromatic heterocyclic group” means that the number of atoms constituting the ring is 5 or 6, and one to plural atoms in the atoms constituting the ring. Means a monovalent group derived by removing one hydrogen atom at any position from a non-aromatic ring containing a heteroatom. Specific examples include pyrrolidinyl group, piperazinyl group, piperidinyl group, morpholinyl group, tetrahydrofuryl group, tetrahydropyranyl group and the like.
　本明細書において使用する「置換基を１個又は２個有していてもよい」とは、置換可能な部位に、任意に組み合わせて１又は２個の置換基を有してもよいことを意味する。 As used herein, “may have one or two substituents” means that one or two substituents may be arbitrarily combined at substitutable sites. means.
　Ｒ1は、水素原子、ハロゲン原子、アミノ基、Ｃ1-6アルキル基、Ｃ1-6アルコキシ基、Ｃ1-6アルキルアミノ基、ヒドロキシＣ1-6アルキルアミノ基又はＣ1-6アルコキシＣ1-6アルキル基を意味し、特に、水素原子、アミノ基、又はＣ1-6アルコキシＣ1-6アルキル基が好ましく、該Ｃ1-6アルコキシＣ1-6アルキル基としては、メトキシメチル基が好ましい。 R 1 is a hydrogen atom, halogen atom, amino group, C 1-6 alkyl group, C 1-6 alkoxy group, C 1-6 alkylamino group, hydroxy C 1-6 alkylamino group or C 1-6 alkoxy C 1-6 alkyl group means a hydrogen atom, an amino group, or a C 1-6 alkoxy C 1-6 alkyl group, and the C 1-6 alkoxy C 1-6 alkyl group is preferably a methoxymethyl group Is preferred.
　Ｒは、水素原子、メチル基、エチル基、又は２－ジメチルアミノエチル基が好ましい。 R is preferably a hydrogen atom, a methyl group, an ethyl group, or a 2-dimethylaminoethyl group.
　Ｘ及びＹの一方は、窒素原子を、他方は、窒素原子又は酸素原子を意味する。 One of X and Y means a nitrogen atom, and the other means a nitrogen atom or an oxygen atom.
　Ｘ及びＹを含む下式（ＩＩ） The following formula (II) including X and Y
Figure JPOXMLDOC01-appb-C000013
Figure JPOXMLDOC01-appb-C000013
で表される部分構造は、下記に表されるような構造を有し、それぞれ左端が単結合を介してピリジン環の３位に結合し、かつ、右端はメチレン基を介してＡ環に結合する場合が好ましい。 Have a structure as shown below, and the left end is bonded to the 3-position of the pyridine ring via a single bond, and the right end is bonded to the A ring via a methylene group. This is preferable.
Figure JPOXMLDOC01-appb-C000014
Figure JPOXMLDOC01-appb-C000014
　例えば、式（ＩＩＩ）の部分構造を有する場合は、本発明化合物の親化合物、つまり、γ－グルタミルアミノ基が導入される前の化合物の構造は下式のようになる。 For example, when it has a partial structure of the formula (III), the parent compound of the compound of the present invention, that is, the structure of the compound before the γ-glutamylamino group is introduced is represented by the following formula.
Figure JPOXMLDOC01-appb-C000015
Figure JPOXMLDOC01-appb-C000015
　Ｘ及びＹは、その一方が窒素原子で、他方が酸素原子である場合、又は、Ｘ及びＹとがともに窒素原子である場合が好ましく、Ｘ及びＹの一方が窒素原子で、他方が酸素原子である場合は、Ｘ及びＹを含む下式（ＩＩ） X and Y are preferably a nitrogen atom and the other is an oxygen atom, or both X and Y are preferably a nitrogen atom, and one of X and Y is a nitrogen atom and the other is an oxygen atom. In which X and Y include the following formula (II)
Figure JPOXMLDOC01-appb-C000016
Figure JPOXMLDOC01-appb-C000016
で表される部分構造は、下式（ＩＩＩ）若しくは（ＩＶ）に表されるような構造を有し、それぞれ左端が単結合を介してピリジン環の３位に結合し、かつ、右端はメチレン基を介してＡ環に結合する場合が好ましく、 Have a structure represented by the following formula (III) or (IV), the left end is bonded to the 3-position of the pyridine ring via a single bond, and the right end is methylene. Preferred is the case of bonding to the A ring through a group,
Figure JPOXMLDOC01-appb-C000017
Figure JPOXMLDOC01-appb-C000017
また、Ｘ及びＹとが、ともに窒素原子である場合は、Ｘ及びＹを含む下式（ＩＩ） When both X and Y are nitrogen atoms, the following formula (II) containing X and Y
Figure JPOXMLDOC01-appb-C000018
Figure JPOXMLDOC01-appb-C000018
で表される部分構造は、下式（Ｖ）若しくは（ＶＩ）に表されるような構造を有し、それぞれ左端が単結合を介してピリジン環の３位に結合し、かつ、右端はメチレン基を介してＡ環に結合する場合が好ましい。 Have a structure represented by the following formula (V) or (VI), the left end is bonded to the 3-position of the pyridine ring via a single bond, and the right end is methylene. The case where it couple | bonds with A ring through group is preferable.
Figure JPOXMLDOC01-appb-C000019
Figure JPOXMLDOC01-appb-C000019
　環Ａは、ハロゲン原子若しくはＣ1-6アルキル基を１個若しくは２個を有していてもよい、５若しくは６員のへテロアリール環又はベンゼン環を意味し、ピリジン環、ベンゼン環、フラン環、チオフェン環、又はピロール環である場合が好ましく、さらに、ピリジン環、ベンゼン環、又はチオフェン環が好ましく、特に、ピリジン環又はベンゼン環が好ましい。 Ring A means a 5- or 6-membered heteroaryl ring or benzene ring which may have one or two halogen atoms or C 1-6 alkyl groups, and is a pyridine ring, benzene ring, furan ring , A thiophene ring, or a pyrrole ring, preferably a pyridine ring, a benzene ring, or a thiophene ring, and more preferably a pyridine ring or a benzene ring.
　Ｚは、単結合、メチレン基、エチレン基、酸素原子、硫黄原子、－ＣＨ2Ｏ－、－ＯＣＨ2－、－ＮＨ－、－ＮＨＣＨ2－、－ＣＨ2ＮＨ－、－ＣＨ2Ｓ－、又は－ＳＣＨ2－を意味し、このうちメチレン基、酸素原子、－ＣＨ2Ｏ－、又は－ＯＣＨ2－が好ましく、特に、酸素原子、－ＣＨ2Ｏ－、又は－ＯＣＨ2－が好ましい。 Z represents a single bond, a methylene group, an ethylene group, an oxygen atom, a sulfur atom, -CH 2 O -, - OCH 2 -, - NH -, - NHCH 2 -, - CH 2 NH -, - CH 2 S-, Or —SCH 2 —, among which a methylene group, an oxygen atom, —CH 2 O—, or —OCH 2 — is preferable, and an oxygen atom, —CH 2 O—, or —OCH 2 — is particularly preferable.
　Ｒ3は、水素原子、ハロゲン原子、又は、それぞれ置換基群αから選ばれる置換基を１個又は２個有していてもよい、Ｃ1-6アルキル基、Ｃ3-8シクロアルキル基、Ｃ6-10アリール基、又は５若しくは６員環へテロアリール基を意味する。
［置換基群α］
ハロゲン原子、シアノ基、Ｃ1-6アルキル基、Ｃ1-6アルコキシ基、Ｃ1-6アルコキシカルボニル基、Ｃ3-8シクロアルキル基、Ｃ2-6アルケニル基、及びＣ2-6アルキニル基 R 3 is a hydrogen atom, a halogen atom, or a C 1-6 alkyl group, a C 3-8 cycloalkyl group, each optionally having one or two substituents selected from substituent group α, A C 6-10 aryl group, or a 5- or 6-membered heteroaryl group.
[Substituent group α]
Halogen atom, cyano group, C 1-6 alkyl group, C 1-6 alkoxy group, C 1-6 alkoxycarbonyl group, C 3-8 cycloalkyl group, C 2-6 alkenyl group, and C 2-6 alkynyl group
　Ｒ3として好ましい基を例示すると、ｎ－ブチル基、シクロプロピル基、フェニル基、フルオロフェニル基、フリル基、クロロフリル基、メチルフリル基、チエニル基、ブロモチエニル基、メチルチエニル基、ピリジル基、又はメチルピリジル基が挙げられ、特に、ｎ－ブチル基、シクロプロピル基、フェニル基、フルオロフェニル基、ピリジル基、又はメチルピリジル基が好ましい。 Examples of preferred groups as R 3 include n-butyl group, cyclopropyl group, phenyl group, fluorophenyl group, furyl group, chlorofuryl group, methylfuryl group, thienyl group, bromothienyl group, methylthienyl group, pyridyl group, Or a methylpyridyl group, and an n-butyl group, a cyclopropyl group, a phenyl group, a fluorophenyl group, a pyridyl group, or a methylpyridyl group is particularly preferable.
　Ｚ及びＲ3は、それぞれ任意の組合せにより、環Ａの置換基を構成することができる。そのように構成される環Ａの置換基としてのＲ3－Ｚ－の好ましい例を例示すると、フェノキシ基、ベンジルオキシ基、２－フルオロ－ベンジルオキシ基、３－フルオロ－ベンジルオキシ基、４－フルオロ－ベンジルオキシ基、ピリジン－２－イルオキシメチル基、６－メチル－ピリジン－２－イルオキシメチル基、ピリジン－２－イルメトキシ基、６－メチル－ピリジン－２－イルメトキシ基、４－メチル－ピリジン－２－イルメトキシ基、ブトキシメチル基、又はシクロプロピルメトキシ基が挙げられる。 Z and R 3 can form a substituent of ring A by any combination. Preferable examples of R 3 —Z— as the substituent of ring A thus constituted include phenoxy group, benzyloxy group, 2-fluoro-benzyloxy group, 3-fluoro-benzyloxy group, 4- Fluoro-benzyloxy group, pyridin-2-yloxymethyl group, 6-methyl-pyridin-2-yloxymethyl group, pyridin-2-ylmethoxy group, 6-methyl-pyridin-2-ylmethoxy group, 4-methyl- Examples include a pyridin-2-ylmethoxy group, a butoxymethyl group, or a cyclopropylmethoxy group.
　本明細書において使用する「塩」としては、例えば、無機酸との塩、有機酸との塩、無機塩基との塩、有機塩基との塩、酸性アミノ酸との塩、塩基性アミノ酸との塩等が挙げられ、中でも薬理学的に許容される塩が好ましい。そして、この塩を形成する酸または塩基の数は、特に制限されない。また、本発明に係る化合物の塩にはその塩の無水物と水和物等のその塩の溶媒和物とが包含される。 As used herein, the “salt” includes, for example, a salt with an inorganic acid, a salt with an organic acid, a salt with an inorganic base, a salt with an organic base, a salt with an acidic amino acid, and a salt with a basic amino acid. Among them, pharmacologically acceptable salts are preferable. The number of acids or bases that form this salt is not particularly limited. Further, the salt of the compound according to the present invention includes an anhydride of the salt and a solvate of the salt such as a hydrate.
　無機酸との塩の好ましい例としては、例えば、塩酸、臭化水素酸、硫酸、硝酸、リン酸等との塩が挙げられ、有機酸との塩の好ましい例としては、例えば酢酸、コハク酸、フマル酸、マレイン酸、酒石酸、クエン酸、乳酸、ステアリン酸、安息香酸、メタンスルホン酸、エタンスルホン酸、トリフルオロ酢酸、ｐ－トルエンスルホン酸等との塩が挙げられる。無機塩基との塩の好ましい例としては、例えば、リチウム塩、ナトリウム塩、カリウム塩、カルシウム塩等の塩が挙げられる。有機塩基との塩の好ましい例としては、例えば、メチルアミン、エチルアミン、t-ブチルアミン、トリエチルアミン、ピペリジン、モルホリン等との塩が挙げられる。 Preferable examples of the salt with inorganic acid include salts with hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid and the like, and preferable examples of the salt with organic acid include, for example, acetic acid and succinic acid. And salts with fumaric acid, maleic acid, tartaric acid, citric acid, lactic acid, stearic acid, benzoic acid, methanesulfonic acid, ethanesulfonic acid, trifluoroacetic acid, p-toluenesulfonic acid and the like. Preferable examples of the salt with an inorganic base include salts such as lithium salt, sodium salt, potassium salt and calcium salt. Preferable examples of the salt with an organic base include salts with methylamine, ethylamine, t-butylamine, triethylamine, piperidine, morpholine and the like.
　酸性アミノ酸との塩の好ましい例としては、例えば、アスパラギン酸、グルタミン酸等との塩が挙げられ、塩基性アミノ酸との塩の好ましい例としては、例えば、アルギニン、リジン、オルニチン等との塩が挙げられる。 Preferable examples of the salt with acidic amino acid include salts with aspartic acid, glutamic acid and the like, and preferable examples of the salt with basic amino acid include salts with arginine, lysine, ornithine and the like. It is done.
　本明細書において使用する「抗真菌剤」は、真菌感染症の予防剤及び／又は治療剤を意味する。 As used herein, “antifungal agent” means a prophylactic and / or therapeutic agent for fungal infection.
　本発明に係る化合物は、慣用されている方法により錠剤、散剤、細粒剤、顆粒剤、被覆錠剤、カプセル剤、シロップ剤、トローチ剤、吸入剤、坐剤、注射剤、軟膏剤、眼軟膏剤、テープ剤、点眼剤、点鼻剤、点耳剤、パップ剤、ローション剤等として製剤化することができる。 The compound according to the present invention is prepared by a conventional method in the form of tablets, powders, fine granules, granules, coated tablets, capsules, syrups, troches, inhalants, suppositories, injections, ointments, ophthalmic ointments. It can be formulated as an agent, a tape, an eye drop, a nose drop, an ear drop, a poultice, a lotion or the like.
　製剤化には通常用いられる賦形剤、結合剤、滑沢剤、着色剤、矯味矯臭剤や、及び必要により安定化剤、乳化剤、吸収促進剤、界面活性剤、ｐＨ調整剤、防腐剤、抗酸化剤等を使用することができ、一般に医薬品製剤の原料として用いられる成分を配合して常法により製剤化される。例えば経口製剤を製造するには、本発明に係る化合物と賦形剤、さらに必要に応じて結合剤、崩壊剤、滑沢剤、着色剤、矯味矯臭剤等を加えた後、常法により散剤、細粒剤、顆粒剤、錠剤、被覆錠剤、カプセル剤等とする。 Excipients, binders, lubricants, colorants, flavoring agents, and stabilizers, emulsifiers, absorption promoters, surfactants, pH adjusters, preservatives, if necessary Antioxidants and the like can be used, and they are generally formulated by blending ingredients used as raw materials for pharmaceutical preparations. For example, in order to produce an oral preparation, a compound and an excipient according to the present invention, and a binder, a disintegrant, a lubricant, a coloring agent, a flavoring agent, etc. are added as necessary, and then powdered by a conventional method. , Fine granules, granules, tablets, coated tablets, capsules and the like.
　これらの成分としては、例えば、大豆油、牛脂、合成グリセライド等の動植物油；例えば、流動パラフィン、スクワラン、固形パラフィン等の炭化水素；例えば、ミリスチン酸オクチルドデシル、ミリスチン酸イソプロピル等のエステル油；例えば、セトステアリルアルコール、ベヘニルアルコール等の高級アルコール；シリコン樹脂；シリコン油；例えば、ポリオキシエチレン脂肪酸エステル、ソルビタン脂肪酸エステル、グリセリン脂肪酸エステル、ポリオキシエチレンソルビタン脂肪酸エステル、ポリオキシエチレン硬化ひまし油、ポリオキシエチレンポリオキシプロピレンブロックコポリマー等の界面活性剤；例えば、ヒドロキシエチルセルロース、ポリアクリル酸、カルボキシビニルポリマー、ポリエチレングリコール、ポリビニルピロリドン、メチルセルロース等の水溶性高分子；例えば、エタノール、イソプロパノール等の低級アルコール；例えば、グリセリン、プロピレングリコール、ジプロピレングリコール、ソルビトール等の多価アルコール；例えば、グルコース、ショ糖等の糖；例えば、無水ケイ酸、ケイ酸アルミニウムマグネシウム、ケイ酸アルミニウム等の無機粉体、精製水等が挙げられる。賦形剤としては、例えば、乳糖、コーンスターチ、白糖、ブドウ糖、マンニトール、ソルビット、結晶セルロース、二酸化ケイ素等が、結合剤としては、例えば、ポリビニルアルコール、ポリビニルエーテル、メチルセルロース、エチルセルロース、アラビアゴム、トラガント、ゼラチン、シェラック、ヒドロキシプロピルメチルセルロース、ヒドロキシプロピルセルロース、ポリビニルピロリドン、ポリプロピレングリコール・ポリオキシエチレン・ブロックポリマー、メグルミン等が、崩壊剤としては、例えば、澱粉、寒天、ゼラチン末、結晶セルロース、炭酸カルシウム、炭酸水素ナトリウム、クエン酸カルシウム、デキストリン、ペクチン、カルボキシメチルセルロース・カルシウム等が、滑沢剤としては、例えば、ステアリン酸マグネシウム、タルク、ポリエチレングリコール、シリカ、硬化植物油等が、着色剤としては医薬品に添加することが許可されているものが、矯味矯臭剤としては、例えば、ココア末、ハッカ脳、芳香散、ハッカ油、竜脳、桂皮末等が用いられる。これらの錠剤・顆粒剤には糖衣、その他、必要により適宜コーティングすることはもちろん差支えない。また、シロップ剤や注射用製剤等の液剤を製造する際には、本発明に係る化合物にｐＨ調整剤、溶解剤、等張化剤等と、必要に応じて溶解補助剤、安定化剤等を加えて、常法により製剤化する。外用剤を製造する際の方法は限定されず、常法により製造することができる。すなわち、製剤化にあたり使用する基剤原料としては、医薬品、医薬部外品、化粧品等に通常使用される各種原料を用いることが可能である。使用する基剤原料として具体的には、例えば、動植物油、鉱物油、エステル油、ワックス類、高級アルコール類、脂肪酸類、シリコン油、界面活性剤、リン脂質類、アルコール類、多価アルコール類、水溶性高分子類、粘土鉱物類、精製水等の原料が挙げられ、さらに必要に応じ、例えば、ｐＨ調整剤、抗酸化剤、キレート剤、防腐防黴剤、着色料、香料等を添加することができるが、本発明に係る外用剤の基剤原料はこれらに限定されない。また、必要に応じて分化誘導作用を有する成分、血流促進剤、殺菌剤、消炎剤、細胞賦活剤、ビタミン類、アミノ酸、保湿剤、角質溶解剤等の成分を配合することもできる。なお、上記基剤原料の添加量は、通常外用剤の製造にあたり設定される濃度になる量である。 Examples of these components include animal and vegetable oils such as soybean oil, beef tallow and synthetic glycerides; hydrocarbons such as liquid paraffin, squalane and solid paraffin; for example, ester oils such as octyldodecyl myristate and isopropyl myristate; , Cetostearyl alcohol, higher alcohol such as behenyl alcohol; silicone resin; silicone oil; for example, polyoxyethylene fatty acid ester, sorbitan fatty acid ester, glycerin fatty acid ester, polyoxyethylene sorbitan fatty acid ester, polyoxyethylene hydrogenated castor oil, polyoxyethylene poly Surfactants such as oxypropylene block copolymers; for example, hydroxyethyl cellulose, polyacrylic acid, carboxyvinyl polymer, polyethylene glycol, polyvinyl chloride Water-soluble polymers such as lupyrrolidone and methylcellulose; lower alcohols such as ethanol and isopropanol; polyhydric alcohols such as glycerin, propylene glycol, dipropylene glycol and sorbitol; sugars such as glucose and sucrose; Inorganic powder such as silicic anhydride, magnesium magnesium silicate, aluminum silicate, purified water, and the like. Examples of excipients include lactose, corn starch, sucrose, glucose, mannitol, sorbitol, crystalline cellulose, and silicon dioxide.Examples of binders include polyvinyl alcohol, polyvinyl ether, methyl cellulose, ethyl cellulose, gum arabic, tragacanth, Gelatin, shellac, hydroxypropylmethylcellulose, hydroxypropylcellulose, polyvinylpyrrolidone, polypropylene glycol / polyoxyethylene block polymer, meglumine, etc. are disintegrants such as starch, agar, gelatin powder, crystalline cellulose, calcium carbonate, carbonic acid Sodium hydride, calcium citrate, dextrin, pectin, carboxymethylcellulose calcium, etc. Nesium, talc, polyethylene glycol, silica, hydrogenated vegetable oil, etc. are permitted to be added to pharmaceuticals as coloring agents. Examples of flavoring agents include cocoa powder, mint brain, aroma powder, mint oil. Borneolum, cinnamon powder, etc. are used. Of course, these tablets and granules may be appropriately coated with sugar coating, etc. if necessary. In addition, when producing liquids such as syrups and injectable preparations, the compounds according to the present invention are adjusted to pH adjusters, solubilizers, tonicity agents, etc., and if necessary, solubilizers, stabilizers, etc. And is formulated by a conventional method. The method for producing the external preparation is not limited and can be produced by a conventional method. That is, as a base material used for formulation, various raw materials usually used for pharmaceuticals, quasi drugs, cosmetics, and the like can be used. Specific examples of the base material to be used include animal and vegetable oils, mineral oils, ester oils, waxes, higher alcohols, fatty acids, silicone oils, surfactants, phospholipids, alcohols, and polyhydric alcohols. , Raw materials such as water-soluble polymers, clay minerals, purified water and the like, and if necessary, for example, pH adjusters, antioxidants, chelating agents, antiseptic / antifungal agents, coloring agents, fragrances, etc. However, the base material of the external preparation according to the present invention is not limited thereto. Moreover, components having differentiation-inducing action, blood flow promoters, bactericides, anti-inflammatory agents, cell activators, vitamins, amino acids, humectants, keratolytic agents, and the like can be blended as necessary. In addition, the addition amount of the said base raw material is an amount used as the density | concentration normally set in manufacture of an external preparation.
　本発明に係る化合物又はその塩を投与する場合、その形態は特に限定されず、通常用いられる方法により経口投与でも非経口投与でもよい。例えば、錠剤、散剤、顆粒剤、カプセル剤、シロップ剤、トローチ剤、吸入剤、坐剤、注射剤、軟膏剤、眼軟膏剤、テープ剤、点眼剤、点鼻剤、点耳剤、パップ剤、ローション剤等の剤として製剤化し、投与することができる。 When the compound according to the present invention or a salt thereof is administered, the form is not particularly limited, and it may be administered orally or parenterally by a commonly used method. For example, tablets, powders, granules, capsules, syrups, troches, inhalants, suppositories, injections, ointments, eye ointments, tapes, eye drops, nasal drops, ear drops, poultices It can be formulated and administered as an agent such as a lotion.
　本発明に係る医薬の投与量は、症状の程度、年齢、性別、体重、投与形態・塩の種類、疾患の具体的な種類等に応じて適宜選ぶことができる。 The dosage of the medicament according to the present invention can be appropriately selected according to the degree of symptoms, age, sex, body weight, dosage form / salt type, specific type of disease, and the like.
　投与量は患者の、疾患の種類、症状の程度、患者の年齢、性差、薬剤に対する感受性差等により著しく異なるが、経口剤の場合は、通常成人として１日あたり、１－１００００ｍｇ、好ましくは１０－２０００ｍｇを１日１－数回に分けて投与する。注射剤の場合は、通常成人として１日あたり、通常０．１ｍｇ－１００００ｍｇであり、好ましくは１ｍｇ－２０００ｍｇである。 The dose varies greatly depending on the patient's disease type, symptom severity, patient age, sex difference, sensitivity to the drug, etc. In the case of oral preparations, it is usually 1 to 10,000 mg, preferably 10 -2000 mg is administered 1 to several times a day. In the case of an injection, it is usually 0.1 mg to 10000 mg, preferably 1 mg to 2000 mg per day as a normal adult.
［一般的製造方法］
　式（Ｉ）で表される化合物（以下、化合物（Ｉ）という。）の製造方法について説明する。 [General manufacturing method]
A method for producing a compound represented by formula (I) (hereinafter referred to as compound (I)) will be described.
［製造方法１］　化合物（Ｉ）の製造方法 [Production Method 1] Method for producing Compound (I)
Figure JPOXMLDOC01-appb-C000020
Figure JPOXMLDOC01-appb-C000020
〔式中、環Ａ、Ｒ1、Ｒ3、Ｒ4、Ｘ、Ｙ、Ｚ、及びＲは前記定義と同意義を意味する。〕 [Wherein, ring A, R 1 , R 3 , R 4 , X, Y, Z, and R are as defined above]. ]
　化合物（１－１）は、後述する参考例等に記載の方法を用いて製造することができる。また、化合物（１－１）は米国特許公報ＵＳ２００７/０１０５９０４　Ａ１公報に記載された方法等により製造することもできる。化合物（１－１－２）は、市販品をそのまま用いることもでき、市販品から公知の方法で製造することもできる。 Compound (1-1) can be produced using the method described in Reference Examples described later. Compound (1-1) can also be produced by the method described in US Patent Publication US2007 / 0105904 A1. Compound (1-1-2) may be a commercially available product, or can be produced from a commercially available product by a known method.
［工程１－１］
　本工程は、化合物（１－１－１）を縮合剤の存在下で化合物（１－１－２）と反応させて化合物（１－１－３）を得る工程である。
　本反応に用いる溶媒としては、出発原料をある程度溶解するものであり、かつ、反応を阻害しないものであれば、特に制限はないが、例えば、塩化メチレン、クロロホルム等のハロゲン化炭化水素系溶媒、テトラヒドロフラン、１，４－ジオキサンなどのエーテル系溶媒、Ｎ，Ｎ－ジメチルホルムアミド、Ｎ－メチルピロリジノンなどのアミド系溶媒、ジメチルスルホキシドなどのスルホキシド系溶媒、酢酸エチルなどのエステル系溶媒、アセトニトリル、又はこれらの混合溶媒などを用いることができる。縮合剤としては、Ｂｏｐ（１Ｈ－１，２，３－ベンゾトリアゾール－１－イルオキシ（トリ（ジメチルアミノ））ホスホニウム　ヘキサフルオロホスフェート）、ＨＡＴＵ（Ｏ－（７－アザベンゾトリアゾール－１－イル）－Ｎ，Ｎ，Ｎ’，Ｎ’－テトラメチルウロニウム　ヘキサフルオロホスフェート）、ＷＳＣ（１－エチル－３－（３－ジメチルアミノプロピル）カルボジイミド・塩酸塩）、ＤＣＣ（Ｎ，Ｎ－ジシクロヘキシルカルボジイミド）などを用いることができる。反応を促進するために、触媒量の４－ジメチルアミノピリジンを加えることもできる。また、本工程は、トリエチルアミンやＮ－メチルモルホリンなどの塩基を１当量から３当量加えて行うこともできる。化合物（１－１－２）は化合物（１－１－１）に対して１当量から溶媒量用いることができ、好ましくは溶媒量用いる。縮合剤は化合物（１－１－１）に対して１当量から３当量用いることができ、好ましくは１当量から１．５当量用いる。反応温度は０℃から還流温度であり、反応時間は１０分間から４８時間である。 [Step 1-1]
This step is a step of obtaining compound (1-1-3) by reacting compound (1-1-1) with compound (1-1-2) in the presence of a condensing agent.
The solvent used in this reaction is not particularly limited as long as it dissolves the starting materials to some extent and does not inhibit the reaction. For example, halogenated hydrocarbon solvents such as methylene chloride and chloroform, Ether solvents such as tetrahydrofuran and 1,4-dioxane, amide solvents such as N, N-dimethylformamide and N-methylpyrrolidinone, sulfoxide solvents such as dimethyl sulfoxide, ester solvents such as ethyl acetate, acetonitrile, or these A mixed solvent or the like can be used. As the condensing agent, Bop (1H-1,2,3-benzotriazol-1-yloxy (tri (dimethylamino)) phosphonium hexafluorophosphate), HATU (O- (7-azabenzotriazol-1-yl)- N, N, N ′, N′-tetramethyluronium hexafluorophosphate), WSC (1-ethyl-3- (3-dimethylaminopropyl) carbodiimide / hydrochloride), DCC (N, N-dicyclohexylcarbodiimide), etc. Can be used. A catalytic amount of 4-dimethylaminopyridine can also be added to accelerate the reaction. This step can also be performed by adding 1 to 3 equivalents of a base such as triethylamine or N-methylmorpholine. Compound (1-1-2) can be used in the amount of 1 equivalent to a solvent amount based on compound (1-1-1), preferably the solvent amount is used. The condensing agent can be used in the amount of 1 to 3 equivalents, preferably 1 to 1.5 equivalents, relative to compound (1-1-1). The reaction temperature is from 0 ° C. to reflux temperature, and the reaction time is from 10 minutes to 48 hours.
［工程１－２］
　本工程は、化合物（１－１－３）を、水素雰囲気下でパラジウム触媒を用いてベンジル基を脱保護し、得られたカルボン酸と化合物（１－１）を縮合剤の存在下で反応させて化合物（１－２）を得る工程である。ベンジル基の脱保護の反応に用いる溶媒としては、出発原料をある程度溶解するものであり、かつ、反応を阻害しないものであれば、特に制限はないが、例えば、テトラヒドロフラン、１，４－ジオキサンなどのエーテル系溶媒、メタノール、エタノールなどのアルコール系溶媒、酢酸エチルなどのエステル系溶媒、又はこれらの混合溶媒などを用いることができる。パラジウム触媒としてはパラジウム－カーボン、水酸化パラジウムなどを用いることができる。縮合反応に用いる溶媒としては、出発原料をある程度溶解するものであり、かつ、反応を阻害しないものであれば、特に制限はないが、例えば、塩化メチレン、クロロホルム等のハロゲン化炭化水素系溶媒、テトラヒドロフラン、１，４－ジオキサンなどのエーテル系溶媒、Ｎ，Ｎ－ジメチルホルムアミド、Ｎ－メチルピロリジノンなどのアミド系溶媒、ジメチルスルホキシドなどのスルホキシド系溶媒、酢酸エチルなどのエステル系溶媒、アセトニトリル、又はこれらの混合溶媒などを用いることができる。縮合剤としては、Ｂｏｐ（１Ｈ－１，２，３－ベンゾトリアゾール－１－イルオキシ（トリ（ジメチルアミノ））ホスホニウム　ヘキサフルオロホスフェート）、ＨＡＴＵ（Ｏ－（７－アザベンゾトリアゾール－１－イル）－Ｎ，Ｎ，Ｎ’，Ｎ’－テトラメチルウロニウム　ヘキサフルオロホスフェート）、ＷＳＣ（１－エチル－３－（３－ジメチルアミノプロピル）カルボジイミド・塩酸塩）、ＤＣＣ（Ｎ，Ｎ－ジシクロヘキシルカルボジイミド）などを用いることができる。反応を促進するために、触媒量の４－ジメチルアミノピリジンを加えることもできる。また、本工程は、トリエチルアミンやＮ－メチルモルホリンなどの塩基を１当量から３当量加えて行うこともできる。化合物（１－１－３）は化合物（１－１）に対して１当量から３当量用いることができる。パラジウム触媒は化合物（１－１）に対して０．０１当量から１当量用いることができる。縮合剤は化合物（１－１）に対して１当量から３当量用いることができる。反応温度は０℃から還流温度であり、反応時間は１０分間から４８時間である。 [Step 1-2]
In this step, compound (1-1-3) is deprotected using a palladium catalyst in a hydrogen atmosphere, and the resulting carboxylic acid and compound (1-1) are reacted in the presence of a condensing agent. In this step, compound (1-2) is obtained. The solvent used for the deprotection reaction of the benzyl group is not particularly limited as long as it dissolves the starting materials to some extent and does not inhibit the reaction. For example, tetrahydrofuran, 1,4-dioxane, etc. Ether solvents, alcohol solvents such as methanol and ethanol, ester solvents such as ethyl acetate, or a mixed solvent thereof can be used. As the palladium catalyst, palladium-carbon, palladium hydroxide, or the like can be used. The solvent used in the condensation reaction is not particularly limited as long as it dissolves the starting materials to some extent and does not inhibit the reaction. For example, halogenated hydrocarbon solvents such as methylene chloride and chloroform, Ether solvents such as tetrahydrofuran and 1,4-dioxane, amide solvents such as N, N-dimethylformamide and N-methylpyrrolidinone, sulfoxide solvents such as dimethyl sulfoxide, ester solvents such as ethyl acetate, acetonitrile, or these A mixed solvent or the like can be used. As the condensing agent, Bop (1H-1,2,3-benzotriazol-1-yloxy (tri (dimethylamino)) phosphonium hexafluorophosphate), HATU (O- (7-azabenzotriazol-1-yl)- N, N, N ′, N′-tetramethyluronium hexafluorophosphate), WSC (1-ethyl-3- (3-dimethylaminopropyl) carbodiimide / hydrochloride), DCC (N, N-dicyclohexylcarbodiimide), etc. Can be used. A catalytic amount of 4-dimethylaminopyridine can also be added to accelerate the reaction. This step can also be performed by adding 1 to 3 equivalents of a base such as triethylamine or N-methylmorpholine. Compound (1-1-3) can be used in the amount of 1 to 3 equivalents based on compound (1-1). The palladium catalyst can be used in the amount of 0.01 to 1 equivalent based on compound (1-1). The condensing agent can be used in the amount of 1 to 3 equivalents based on compound (1-1). The reaction temperature is from 0 ° C. to reflux temperature, and the reaction time is from 10 minutes to 48 hours.
［工程１－３］
　本工程は、化合物（１－２）のｔ－ブトキシカルボニル基を酸性条件下で脱保護して化合物（Ｉ）を得る工程である。本反応に用いる溶媒としては、出発原料をある程度溶解するものであり、かつ、反応を阻害しないものであれば、特に制限はないが、例えば、１，４－ジオキサン、テトラヒドロフランなどのエーテル系溶媒、ベンゼン、トルエン、などの芳香族炭化水素系溶媒、メタノール、エタノールなどのアルコール系溶媒、塩化メチレン、水、又はこれらの混合溶媒などを用いることができる。酸としては塩酸、硫酸、臭化水素酸、トリフルオロ酢酸、ギ酸などを用いることができる。酸は化合物（１－２）に対して２当量から溶媒量用いる。反応温度は０℃から還流温度であり、反応時間は１０分間から２４時間である。 [Step 1-3]
This step is a step of obtaining compound (I) by deprotecting the t-butoxycarbonyl group of compound (1-2) under acidic conditions. The solvent used in this reaction is not particularly limited as long as it dissolves the starting materials to some extent and does not inhibit the reaction. For example, ether solvents such as 1,4-dioxane and tetrahydrofuran, Aromatic hydrocarbon solvents such as benzene and toluene, alcohol solvents such as methanol and ethanol, methylene chloride, water, or a mixed solvent thereof can be used. As the acid, hydrochloric acid, sulfuric acid, hydrobromic acid, trifluoroacetic acid, formic acid and the like can be used. The acid is used in the amount of 2 equivalents to a solvent amount based on compound (1-2). The reaction temperature is from 0 ° C. to reflux temperature, and the reaction time is from 10 minutes to 24 hours.
［製造方法２］　化合物（Ｉａ）の製造方法 [Production Method 2] Method for producing Compound (Ia)
Figure JPOXMLDOC01-appb-C000021
Figure JPOXMLDOC01-appb-C000021
〔式中、環Ａ、Ｒ1、Ｒ3、Ｒ4、Ｘ、Ｙ、及びＺは前記定義と同意義を意味する。〕 [Wherein, ring A, R 1 , R 3 , R 4 , X, Y, and Z are as defined above]. ]
［工程２－１］
　本工程は、化合物（１－１）を縮合剤の存在下でＮ－ｔ－ブトキシカルボニル－Ｌ－グルタミック　アシッド　１－ｔ－ブチルエステルと反応させて化合物（２－１）を得る工程である。[工程１－１]と同様の方法で化合物（２－１）を製造することができる。 [Step 2-1]
In this step, compound (1-1) is reacted with Nt-butoxycarbonyl-L-glutamic acid 1-t-butyl ester in the presence of a condensing agent to obtain compound (2-1). Compound (2-1) can be produced by the same method as in [Step 1-1].
［工程２－２］
　本工程は、化合物（２－１）の２つのｔ－ブトキシカルボニル基を酸性条件下で脱保護して化合物（Ｉａ）を得る工程である。[工程１－３]と同様の方法で化合物（Ｉａ）を製造することができる。 [Step 2-2]
This step is a step of obtaining compound (Ia) by deprotecting two t-butoxycarbonyl groups of compound (2-1) under acidic conditions. Compound (Ia) can be produced in the same manner as in [Step 1-3].
　本発明に係る化合物は、例えば以下の実施例、参考例及び製造例等に記載した方法により製造することができる。ただし、これらは例示的なものであって、本発明に係る化合物は如何なる場合も以下の具体例に限定されるものではない。 The compound according to the present invention can be produced, for example, by the methods described in the following examples, reference examples and production examples. However, these are illustrative, and the compound according to the present invention is not limited to the following specific examples in any case.
［参考例１］３－（３－（４－（ピリジン－２－イルオキシメチル）－ベンジル）－イソキサゾール－５－イル）－ピリジン－２－イルアミン [Reference Example 1] 3- (3- (4- (Pyridin-2-yloxymethyl) -benzyl) -isoxazol-5-yl) -pyridin-2-ylamine
Figure JPOXMLDOC01-appb-C000022
 　製造例１－１－５に記載の（４－（ピリジン－２－イルオキシメチル）－フェニル）－アセトヒドロキシモイル　クロライド（５１０ｍｇ、１．８４ｍｍｏｌ）と国際公開第０７/０５２６１５号公報の製造例１－２－３に記載の３－エチニル－ピリジン－２－イルアミン（１５０ｍｇ、１．２７ｍｍｏｌ）のテトラヒドロフラン（５ｍＬ）溶液に、室温でトリエチルアミン（７０８μＬ、５．０８ｍｍｏｌ）を加え、室温で９５分攪拌した。反応溶液に室温で水を加え、酢酸エチルで抽出した。有機層を飽和食塩水で洗浄し、それを無水硫酸マグネシウムで乾燥し、その溶媒を減圧下留去した。残渣をＮＨシリカゲルカラムクロマトグラフィー（ヘプタン：酢酸エチル＝２：１）で精製し、標記化合物（１２０ｍｇ、２６％）を得た。
1H－NMR  Spectrum  (CDCl3)δ(ppm)：4.08(2H,s),  5.37(2H,s),　6.33(1H,s),  6.45(2H,brs),　6.79－6.82(2H,m),  6.88－6.91(1H,m),  7.30(2H,d,J=8.1Hz),　7.45(2H,d,J=8.1Hz),  7.57－7.61(1H,m),  7.85(1H,d,J=7.3Hz),　8.03(1H,d,J=5.5Hz),  8.17(1H,m).
Figure JPOXMLDOC01-appb-C000022
 (4- (Pyridin-2-yloxymethyl) -phenyl) -acetohydroxymoyl chloride (510 mg, 1.84 mmol) described in Preparation Example 1-1-5 and Preparation Example 1 of WO 07/052615 To a solution of 3-ethynyl-pyridin-2-ylamine (150 mg, 1.27 mmol) described in 2-3 in tetrahydrofuran (5 mL) was added triethylamine (708 μL, 5.08 mmol) at room temperature, and the mixture was stirred at room temperature for 95 minutes. . Water was added to the reaction solution at room temperature, and the mixture was extracted with ethyl acetate. The organic layer was washed with saturated brine, dried over anhydrous magnesium sulfate, and the solvent was evaporated under reduced pressure. The residue was purified by NH silica gel column chromatography (heptane: ethyl acetate = 2: 1) to obtain the title compound (120 mg, 26%).
1 H-NMR Spectrum (CDCl 3 ) δ (ppm): 4.08 (2H, s), 5.37 (2H, s), 6.33 (1H, s), 6.45 (2H, brs), 6.79-6.82 (2H, m) , 6.88-6.91 (1H, m), 7.30 (2H, d, J = 8.1Hz), 7.45 (2H, d, J = 8.1Hz), 7.57-7.61 (1H, m), 7.85 (1H, d, J = 7.3Hz), 8.03 (1H, d, J = 5.5Hz), 8.17 (1H, m).
　出発物質（４－（ピリジン－２－イルオキシメチル）－フェニル）－アセトヒドロキシモイル　クロリドは以下の方法で合成した。 Starting material (4- (pyridin-2-yloxymethyl) -phenyl) -acetohydroxymoyl chloride was synthesized by the following method.
［製造例１－１－１］（４－（ピリジン－２－イルオキシメチル）－フェニル）メタノール [Production Example 1-1-1] (4- (pyridin-2-yloxymethyl) -phenyl) methanol
Figure JPOXMLDOC01-appb-C000023
 　１，４－ベンゼンジメタノール（５．５ｇ、４０ｍｍｏｌ）、２－フルオロピリジン（１．３ｇ、１３ｍｍｏｌ）、及びＮ，Ｎ－ジメチルホルムアミド（１５ｍＬ）の混合物に、０℃で水素化ナトリウム（１．４ｇ、４０ｍｍｏｌ、６６％　ｉｎ　ｏｉｌ）を加え、室温で２０分間と７０℃で１時間攪拌した。反応混合物に水を加え、酢酸エチルで抽出した。有機層を飽和食塩水で洗浄し、その溶媒を減圧下留去した。残渣をＮＨシリカゲルカラムクロマトグラフィー（酢酸エチル：ヘプタン＝１：１）で精製し、標記化合物（１．９ｇ、６６％）を得た。
1H－NMR  Spectrum  (CDCl3)δ(ppm):4.71(2H,s),  5.38(2H,s),　6.81(1H,td,J=0.9,8.4Hz),　6.89(1H,ddd,J=0.9,5.1,7.1Hz),  7.37－7.47(4H,m),  7.59(1H,ddd,J=2.0,7.1,8.3Hz),　8.17(1H,ddd,J=0.7,2.0,5.1Hz).
Figure JPOXMLDOC01-appb-C000023
 To a mixture of 1,4-benzenedimethanol (5.5 g, 40 mmol), 2-fluoropyridine (1.3 g, 13 mmol), and N, N-dimethylformamide (15 mL) at 0 ° C., sodium hydride (1. 4 g, 40 mmol, 66% in oil) was added, and the mixture was stirred at room temperature for 20 minutes and at 70 ° C. for 1 hour. Water was added to the reaction mixture, and the mixture was extracted with ethyl acetate. The organic layer was washed with saturated brine, and the solvent was distilled off under reduced pressure. The residue was purified by NH silica gel column chromatography (ethyl acetate: heptane = 1: 1) to obtain the title compound (1.9 g, 66%).
1 H-NMR Spectrum (CDCl 3 ) δ (ppm): 4.71 (2H, s), 5.38 (2H, s), 6.81 (1H, td, J = 0.9,8.4Hz), 6.89 (1H, ddd, J = 0.9,5.1,7.1Hz), 7.37-7.47 (4H, m), 7.59 (1H, ddd, J = 2.0,7.1,8.3Hz), 8.17 (1H, ddd, J = 0.7,2.0,5.1Hz).
［製造例１－１－２］４－（ピリジン－２－イルオキシメチル）－ベンズアルデヒド [Production Example 1-1-2] 4- (Pyridin-2-yloxymethyl) -benzaldehyde
Figure JPOXMLDOC01-appb-C000024
 　製造例１－１－１に記載の（４－（ピリジン－２－イルオキシメチル）－フェニル）メタノール（１．９ｇ、８．６ｍｍｏｌ）と塩化メチレン（３０ｍＬ）の混合物に、二酸化マンガン（１５ｇ、１７ｍｍｏｌ）を室温で加え、その温度で終夜攪拌した。反応混合物をセライトを用いてろ過し、その溶媒を減圧下留去した。残渣をシリカゲルカラムクロマトグラフィー（酢酸エチル：ヘプタン＝１：４）で精製し、標記化合物（７７０ｍｇ、４２％）を得た。
1H－NMR  Spectrum  (CDCl3)δ(ppm):5.48(2H,s),  6.85(1H,d,J=8.2Hz),　6.90－6.93(1H,m),  7.60－7.64(3H,m),  7.89(2H,d,J=8.1Hz),　8.16(1H,dd,J=1.3,4.9Hz),　10.0(1H,s).
Figure JPOXMLDOC01-appb-C000024
 To a mixture of (4- (pyridin-2-yloxymethyl) -phenyl) methanol (1.9 g, 8.6 mmol) and methylene chloride (30 mL) described in Preparation Example 1-1-1, manganese dioxide (15 g, 17 mmol) was added at room temperature and stirred at that temperature overnight. The reaction mixture was filtered using celite, and the solvent was distilled off under reduced pressure. The residue was purified by silica gel column chromatography (ethyl acetate: heptane = 1: 4) to obtain the title compound (770 mg, 42%).
1 H-NMR Spectrum (CDCl 3 ) δ (ppm): 5.48 (2H, s), 6.85 (1H, d, J = 8.2Hz), 6.90-6.93 (1H, m), 7.60-7.64 (3H, m) , 7.89 (2H, d, J = 8.1Hz), 8.16 (1H, dd, J = 1.3,4.9Hz), 10.0 (1H, s).
［製造例１－１－３］２－（４－（（Ｅ）－２－ニトロ－ビニル）－ベンジルオキシ）－ピリジン [Production Example 1-1-3] 2- (4-((E) -2-nitro-vinyl) -benzyloxy) -pyridine
Figure JPOXMLDOC01-appb-C000025
 　製造例１－１－２に記載の４－（ピリジン－２－イルオキシメチル）－ベンズアルデヒド（２３．４ｇ、１１０ｍｍｏｌ）、ニトロメタン（３３．６ｇ、５５０ｍｍｏl）、酢酸アンモニウム（１７．０ｇ、２２０ｍｍｏｌ）そして酢酸（２００ｍＬ）の混合物を１００℃で１時間４５分撹拌した。反応溶液を氷冷撹拌しながら少量の水を加え、析出した固体をろ取し、標記化合物（２１．０ｇ、７４．５％）を得た。
1H－NMR  Spectrum  (DMSO－d6)δ(ppm):5.41(2H,s),  6.91(1H,dd,J=0.8,8.4Hz),  6.99－7.10(1H,m),  7.53(2H,d,J=8.0Hz),　7.72－7.79(1H,m),  7.86(2H,d,J=8.0Hz),　8.13(1H,d,J=10Hz),　8.15-8.20(1H,m),　8.23(1H,d,J=10Hz).
Figure JPOXMLDOC01-appb-C000025
 4- (Pyridin-2-yloxymethyl) -benzaldehyde (23.4 g, 110 mmol), nitromethane (33.6 g, 550 mmol), ammonium acetate (17.0 g, 220 mmol) described in Preparation Example 1-1-2 and A mixture of acetic acid (200 mL) was stirred at 100 ° C. for 1 hour 45 minutes. A small amount of water was added to the reaction solution while stirring on ice, and the precipitated solid was collected by filtration to obtain the title compound (21.0 g, 74.5%).
1 H-NMR Spectrum (DMSO-d 6 ) δ (ppm): 5.41 (2H, s), 6.91 (1H, dd, J = 0.8, 8.4Hz), 6.99-7.10 (1H, m), 7.53 (2H, d, J = 8.0Hz), 7.72-7.79 (1H, m), 7.86 (2H, d, J = 8.0Hz), 8.13 (1H, d, J = 10Hz), 8.15-8.20 (1H, m), 8.23 (1H, d, J = 10Hz).
［製造例１－１－４］２－(４－（２－ニトロ－エチル）－ベンジルオキシ)－ピリジン [Production Example 1-1-4] 2- (4- (2-nitro-ethyl) -benzyloxy) -pyridine
Figure JPOXMLDOC01-appb-C000026
 　製造例１－１－３に記載の２－（４－（（Ｅ）－２－ニトロ－ビニル）－ベンジルオキシ）－ピリジン（２１．０ｇ、８１．９ｍｍｏｌ）、酢酸（２１ｍＬ）、ジメチルスルホキシド（２００ｍＬ）の溶液に、適宜冷却しながら室温で水素化ホウ素ナトリウム（４．９６ｇ、１３１ｍｍｏｌ）を加えた。水素化ホウ素ナトリウムを加えた後、冷浴を除き室温で１５分間撹拌した。反応溶液を水と酢酸エチルに分配した。酢酸エチル層を水で２回、食塩水で１回洗浄し、それを無水硫酸マグネシウムで乾燥し、その溶媒を減圧下留去した。残渣をＮＨシリカゲルカラムクロマトグラフィー（酢酸エチル：ヘプタン＝１：３）で精製し、標記化合物（１６．３ｇ、７７．１％）を得た。
1H－NMR  Spectrum  (DMSO－d6)δ(ppm):3.23(2H,t,J=6.8Hz),  4.85(2H,t,J=6.8Hz),　5.32(2H,s)　6.82－6.88(1H,m),  6.96－7.01(1H,m),  7.28(2H,d,J=8.0Hz),　7.38(2H,d,J=8.0Hz),  7.69－7.74(1H,m),  8.15－8.19(1H,m).
Figure JPOXMLDOC01-appb-C000026
 2- (4-((E) -2-nitro-vinyl) -benzyloxy) -pyridine (21.0 g, 81.9 mmol), acetic acid (21 mL), dimethyl sulfoxide (described in Preparation Example 1-1-3) 200 mL) was added sodium borohydride (4.96 g, 131 mmol) at room temperature with appropriate cooling. After adding sodium borohydride, the cooling bath was removed and the mixture was stirred at room temperature for 15 minutes. The reaction solution was partitioned between water and ethyl acetate. The ethyl acetate layer was washed twice with water and once with brine, dried over anhydrous magnesium sulfate, and the solvent was evaporated under reduced pressure. The residue was purified by NH silica gel column chromatography (ethyl acetate: heptane = 1: 3) to obtain the title compound (16.3 g, 77.1%).
1 H-NMR Spectrum (DMSO-d 6 ) δ (ppm): 3.23 (2H, t, J = 6.8Hz), 4.85 (2H, t, J = 6.8Hz), 5.32 (2H, s) 6.82-6.88 ( 1H, m), 6.96-7.01 (1H, m), 7.28 (2H, d, J = 8.0Hz), 7.38 (2H, d, J = 8.0Hz), 7.69-7.74 (1H, m), 8.15-8.19 (1H, m).
［製造例１－１－５］４－（ピリジン－２－イルオキシメチル）－フェニル－アセトヒドロキシモイル　クロリド [Production Example 1-1-5] 4- (Pyridin-2-yloxymethyl) -phenyl-acetohydroxymoyl chloride
Figure JPOXMLDOC01-appb-C000027
 　メタノール（７５ｍＬ）にリチウム　ワイアー（３２３ｍｇ、４６．６ｍｍｏｌ）を加え溶解した。その混合溶液に製造例１－１－４に記載の２－(４－（２－ニトロ－エチル）－ベンジルオキシ)－ピリジン（６．０ｇ、２３．３ｍｍｏｌ）を加え、反応溶液を減圧下濃縮した。残渣にトルエンを加え、その溶媒を減圧下濃縮した。得られた残渣の塩化メチレン（９０ｍＬ）とテトラヒドロフラン（４５ｍＬ）の溶液を－７８℃に冷却し、撹拌下にチタニウム（ＩＶ）クロリド（８．１５ｍＬ、７４．４ｍｍｏｌ）を加えた。チタニウム（ＩＶ）クロリドを加え終わったらすぐに反応溶液を１０分間、ついで室温で３０分撹拌した。反応溶液を氷水に展開し酢酸エチルで抽出した。有機層を無水硫酸マグネシウムで乾燥し、硫酸マグネシウムをろ過により取り除いた。ろ液を中性シリカゲルを敷いたグラスフィルター（酢酸エチルで溶出）に通した。得られた溶出液を減圧下濃縮した。残渣に少量の酢酸エチルを加え、析出した固体をろ取し標記化合物（１．８６ｇ、２８．８％）を得た。
1H－NMR  Spectrum  (DMSO－d6)δ(ppm):3.82(2H,s),  5.33(2H,s),　6.84－6.89(1H,m),  6.97－7.01(1H,m),  7.25(2H,d,J=8.4Hz),　7.41(2H,d,J=8.4Hz),  7.70－7.76(1H,m),  8.15-8.18(1H,m),  11.7(1H,s).
Figure JPOXMLDOC01-appb-C000027
 Lithium wire (323 mg, 46.6 mmol) was added to methanol (75 mL) and dissolved. To the mixed solution was added 2- (4- (2-nitro-ethyl) -benzyloxy) -pyridine (6.0 g, 23.3 mmol) described in Preparation Example 1-1-4, and the reaction solution was concentrated under reduced pressure. did. Toluene was added to the residue, and the solvent was concentrated under reduced pressure. A solution of the obtained residue in methylene chloride (90 mL) and tetrahydrofuran (45 mL) was cooled to −78 ° C., and titanium (IV) chloride (8.15 mL, 74.4 mmol) was added with stirring. As soon as the titanium (IV) chloride was added, the reaction solution was stirred for 10 minutes and then at room temperature for 30 minutes. The reaction solution was developed in ice water and extracted with ethyl acetate. The organic layer was dried over anhydrous magnesium sulfate, and magnesium sulfate was removed by filtration. The filtrate was passed through a glass filter (eluted with ethyl acetate) covered with neutral silica gel. The obtained eluate was concentrated under reduced pressure. A small amount of ethyl acetate was added to the residue, and the precipitated solid was collected by filtration to obtain the title compound (1.86 g, 28.8%).
1 H-NMR Spectrum (DMSO-d 6 ) δ (ppm): 3.82 (2H, s), 5.33 (2H, s), 6.84-6.89 (1H, m), 6.97-7.01 (1H, m), 7.25 ( 2H, d, J = 8.4Hz), 7.41 (2H, d, J = 8.4Hz), 7.70-7.76 (1H, m), 8.15-8.18 (1H, m), 11.7 (1H, s).
　また、製造例１－１－５の標記化合物は下記の別法で合成することもできる。 The title compound of Production Example 1-1-5 can also be synthesized by the following alternative method.
[製造例１－２－１]２－（４－ブロモ－ベンジルオキシ）－ピリジン [Production Example 1-2-1] 2- (4-Bromo-benzyloxy) -pyridine
Figure JPOXMLDOC01-appb-C000028
 　４－ブロモベンジルアルコール（２５ｇ、１３０ｍｍｏｌ）のＮ，Ｎ―ジメチルホルムアミド（１２５ｍＬ）溶液に室温でカリウム　ｔｅｒｔ－ブトキシカリウム（１５．８ｇ、１４１ｍｍｏｌ）を加え、５４℃で１０分間撹拌した。４０℃から５８℃で、その反応溶液に２－フルオロピリジン（１５ｍＬ、１５４ｍｍｏｌ）を加え、さらに６５℃で３０分間撹拌した。反応溶液を室温とし、水と酢酸エチルを加え分液した。水層をさらに酢酸エチル（２回）で抽出した。酢酸エチル層を合わせ、水（３回）と食塩水（１回）で洗浄し、無水硫酸マグネシウムで乾燥後、ろ過した。そのろ液を減圧下濃縮した。残渣にジエチルエーテルを加え、減圧下濃縮することにより、標記化合物（３４ｇ）を粗生成物として得た。
1H－NMR  Spectrum  (CDCl3)δ(ppm):5.33(2H,s),  6.87－6.70(1H,m),  6.98－7.02(1H,m)　7.38－7.44(2H,m),  7.55－7.60(2H,m),  7.71－7.76(1H,m),  8.15－8.18(1H,m).
Figure JPOXMLDOC01-appb-C000028
 To a solution of 4-bromobenzyl alcohol (25 g, 130 mmol) in N, N-dimethylformamide (125 mL) was added potassium tert-butoxypotassium (15.8 g, 141 mmol) at room temperature, and the mixture was stirred at 54 ° C. for 10 minutes. 2-Fluoropyridine (15 mL, 154 mmol) was added to the reaction solution at 40 ° C. to 58 ° C., and the mixture was further stirred at 65 ° C. for 30 minutes. The reaction solution was brought to room temperature, and water and ethyl acetate were added for liquid separation. The aqueous layer was further extracted with ethyl acetate (twice). The ethyl acetate layers were combined, washed with water (3 times) and brine (1 time), dried over anhydrous magnesium sulfate, and filtered. The filtrate was concentrated under reduced pressure. Diethyl ether was added to the residue, and the mixture was concentrated under reduced pressure to obtain the title compound (34 g) as a crude product.
1 H-NMR Spectrum (CDCl3) δ (ppm): 5.33 (2H, s), 6.87-6.70 (1H, m), 6.98-7.02 (1H, m) 7.38-7.44 (2H, m), 7.55-7.60 ( 2H, m), 7.71-7.76 (1H, m), 8.15-8.18 (1H, m).
[製造例１－２－２]４－（ピリジン－２－イルオキシメチル）－ベンズアルデヒド [Production Example 1-2-2] 4- (Pyridin-2-yloxymethyl) -benzaldehyde
Figure JPOXMLDOC01-appb-C000029
 　製造例１－２－１に記載の２－（４－ブロモ－ベンジルオキシ）－ピリジン(３４ｇ、１２８ｍｍｏｌ)のテトラヒドロフラン溶液（１２０ｍＬ）に、－７８℃でｎ－ブチルリチウム（５０ｍＬ、２．６Ｍ　ヘキサン溶液、１３４ｍｍｏｌ）を滴下した。３０分撹拌した後、その反応溶液に－７８℃でＮ，Ｎ－ジメチルホルムアミド（１０ｍＬ、１３４ｍｍｏｌ）を滴下し、室温で撹拌した。反応溶液に水と酢酸エチルを加え分液した。酢酸エチル層を水（２回）と食塩水（１回）で洗浄した。水層を合わせ酢酸エチルで抽出した。得られた酢酸エチル層を水（２回）と食塩水（１回）で洗浄した。先に得られた酢酸エチル層と今回得られた酢酸エチル層を合わせ、無水硫酸マグネシウムで乾燥後、ろ過した。そのろ液を減圧下濃縮することにより、標記化合物（２６．８ｇ）を粗生成物として得た。
Figure JPOXMLDOC01-appb-C000029
 To a tetrahydrofuran solution (120 mL) of 2- (4-bromo-benzyloxy) -pyridine (34 g, 128 mmol) described in Preparation Example 1-2-1, n-butyllithium (50 mL, 2.6 M hexane) was added at −78 ° C. Solution, 134 mmol) was added dropwise. After stirring for 30 minutes, N, N-dimethylformamide (10 mL, 134 mmol) was added dropwise to the reaction solution at −78 ° C., and the mixture was stirred at room temperature. Water and ethyl acetate were added to the reaction solution for liquid separation. The ethyl acetate layer was washed with water (twice) and brine (once). The aqueous layers were combined and extracted with ethyl acetate. The obtained ethyl acetate layer was washed with water (twice) and brine (once). The ethyl acetate layer obtained previously and the ethyl acetate layer obtained this time were combined, dried over anhydrous magnesium sulfate, and filtered. The filtrate was concentrated under reduced pressure to obtain the title compound (26.8 g) as a crude product.
[製造例１－２－３]２－（４－（（Ｅ）－２－ニトロ－ビニル）－ベンジルオキシ）－ピリジン [Production Example 1-2-3] 2- (4-((E) -2-nitro-vinyl) -benzyloxy) -pyridine
Figure JPOXMLDOC01-appb-C000030
 　製造例１－２－２に記載の４－（ピリジン－２－イルオキシメチル）－ベンズアルデヒド（２６．８ｇ、１２６ｍｍｏｌ）、ニトロメタン（３４ｍＬ、６３０ｍｍｏl）、酢酸アンモニウム（１９ｇ、２５２ｍｍｏｌ）及び酢酸（９０ｍＬ）の混合物を１００℃で１時間３０分撹拌した。反応溶液に酢酸エチルと水を加え分液した。その有機層を分離し、水（５回）と飽和重曹水（１回）で洗浄し、無水硫酸マグネシウムで乾燥後、ろ過した。そのろ液を減圧下濃縮することにより、標記化合物（３１ｇ）を粗生成物として得た。
Figure JPOXMLDOC01-appb-C000030
 4- (Pyridin-2-yloxymethyl) -benzaldehyde (26.8 g, 126 mmol), nitromethane (34 mL, 630 mmol), ammonium acetate (19 g, 252 mmol) and acetic acid (90 mL) described in Preparation Example 1-2-2 The mixture was stirred at 100 ° C. for 1 hour 30 minutes. Ethyl acetate and water were added to the reaction solution for liquid separation. The organic layer was separated, washed with water (5 times) and saturated aqueous sodium hydrogen carbonate (once), dried over anhydrous magnesium sulfate and filtered. The filtrate was concentrated under reduced pressure to obtain the title compound (31 g) as a crude product.
[製造例１－２－４]２－(４－（２－ニトロ－エチル）－ベンジルオキシ)－ピリジン [Production Example 1-2-4] 2- (4- (2-Nitro-ethyl) -benzyloxy) -pyridine
Figure JPOXMLDOC01-appb-C000031
 　製造例１－２－３に記載の２－（４－（（Ｅ）－２－ニトロ－ビニル）－ベンジルオキシ）－ピリジン（３０．８ｇ、１２０ｍｍｏｌ）と酢酸（７．４ｍＬ）のジメチルスルホキシド（１５０ｍＬ）の溶液に、３０℃以下で水素化ホウ素ナトリウム（２．４５ｇ、６４．８ｍｍｏｌ）を加えた。反応溶液を室温で４０分間撹拌した。反応溶液に３０℃以下で水と酢酸エチルとジエチルエーテルを加え、水と有機層に分配した。水層を酢酸エチルで抽出した。先に得られた有機層と酢酸エチル層合わせ、水（３回）と食塩水（１回）で洗浄し、無水硫酸マグネシウムで乾燥後、ろ過した。そのろ液を減圧下濃縮した。残渣をＮＨシリカゲルカラムクロマトグラフィー（酢酸エチル：ヘプタン＝１：４）で精製し、標記化合物（１５．２ｇ）を得た。
Figure JPOXMLDOC01-appb-C000031
 Dimethyl sulfoxide of 2- (4-((E) -2-nitro-vinyl) -benzyloxy) -pyridine (30.8 g, 120 mmol) and acetic acid (7.4 mL) described in Preparation Example 1-2-3 ( 150 mL) solution was added sodium borohydride (2.45 g, 64.8 mmol) at 30 ° C. or lower. The reaction solution was stirred at room temperature for 40 minutes. Water, ethyl acetate and diethyl ether were added to the reaction solution at 30 ° C. or lower, and the mixture was partitioned between water and the organic layer. The aqueous layer was extracted with ethyl acetate. The organic layer and ethyl acetate layer obtained previously were combined, washed with water (3 times) and brine (1 time), dried over anhydrous magnesium sulfate, and then filtered. The filtrate was concentrated under reduced pressure. The residue was purified by NH silica gel column chromatography (ethyl acetate: heptane = 1: 4) to obtain the title compound (15.2 g).
[製造例１－２－５]４－（ピリジン－２－イルオキシメチル）－フェニル－アセトヒドロキシモイル　クロリド [Production Example 1-2-5] 4- (Pyridin-2-yloxymethyl) -phenyl-acetohydroxymoyl chloride
Figure JPOXMLDOC01-appb-C000032
 　製造例１－２－４に記載の２－(４－（２－ニトロ－エチル）－ベンジルオキシ)－ピリジン（１５．２ｇ、５９ｍｍｏｌ）のメタノール（８０ｍＬ）溶液にリチウムメトキシド（４．４９ｇ，１１８ｍｍｏｌ）を加え、３分間撹拌した。反応溶液を減圧下濃縮した。残渣にトルエンを加え、その溶媒を減圧下濃縮した。得られた残渣の塩化メチレン（１００ｍＬ）とテトラヒドロフラン（５０ｍＬ）の溶液を－６６℃に冷却し、撹拌下にチタニウム（ＩＶ）クロリド（２０．８ｍＬ、１８９ｍｍｏｌ）を加えた。反応溶液を０℃で１０分間撹拌し、ついで室温で３０分撹拌した。反応溶液を氷水に注ぎ、室温で３０分撹拌した。反応溶液に酢酸エチルとジエチルエーテルを加え分液した。有機層を水（３回）と食塩水（１回）で洗浄した。水層を合わせ酢酸エチル（２回）で抽出した。酢酸エチル層を合わせ水（３回）と食塩水（１回）で洗浄した。先の有機層と酢酸エチル層を合わせ、無水硫酸マグネシウムと硫酸ナトリウムで乾燥し、ろ過した。そのろ液を減圧下濃縮することにより、標記化合物（１１．５ｇ）を粗生成物として得た。
Figure JPOXMLDOC01-appb-C000032
 To a solution of 2- (4- (2-nitro-ethyl) -benzyloxy) -pyridine (15.2 g, 59 mmol) described in Preparation Example 1-2-4 in methanol (80 mL), lithium methoxide (4.49 g, 118 mmol) was added and stirred for 3 minutes. The reaction solution was concentrated under reduced pressure. Toluene was added to the residue, and the solvent was concentrated under reduced pressure. A solution of the obtained residue in methylene chloride (100 mL) and tetrahydrofuran (50 mL) was cooled to −66 ° C., and titanium (IV) chloride (20.8 mL, 189 mmol) was added with stirring. The reaction solution was stirred at 0 ° C. for 10 minutes and then at room temperature for 30 minutes. The reaction solution was poured into ice water and stirred at room temperature for 30 minutes. Ethyl acetate and diethyl ether were added to the reaction solution for liquid separation. The organic layer was washed with water (3 times) and brine (1 time). The aqueous layers were combined and extracted with ethyl acetate (twice). The ethyl acetate layers were combined and washed with water (3 times) and brine (1 time). The previous organic layer and ethyl acetate layer were combined, dried over anhydrous magnesium sulfate and sodium sulfate, and filtered. The filtrate was concentrated under reduced pressure to obtain the title compound (11.5 g) as a crude product.
　また、参考例１の標記化合物は下記の別法１から３で合成することもできる。 The title compound of Reference Example 1 can also be synthesized by the following alternative methods 1 to 3.
［参考例１の別法１］３－（３－（４－（ピリジン－２－イルオキシメチル）－ベンジル）－イソキサゾール－５－イル）－ピリジン－２－イルアミン [Alternative Method 1 of Reference Example 1] 3- (3- (4- (Pyridin-2-yloxymethyl) -benzyl) -isoxazol-5-yl) -pyridin-2-ylamine
Figure JPOXMLDOC01-appb-C000033
 　塩化亜鉛（８．８２ｇ）とテトラヒドロフラン（１３０ｍＬ）の混合物に、０℃で、国際公開第０７/０５２６１５号公報の製造例１－２－３に記載の３－エチニル－ピリジン－２－イルアミン（３．００ｇ、純度９８％）と、製造例１－２－５に記載の４－（ピリジン－２－イルオキシメチル）－フェニル－アセトヒドロキシモイル　クロリド（１７．４ｇ、純度９４％）を加えた。反応混合物を室温とし、水浴を用いて内温を２８℃以下に保ちながらトリエチルアミン（９．０２ｍＬ）を滴下した。反応混合物を室温で２０分間攪拌し、次いで３５℃で１時間攪拌した。反応混合物を室温とし、反応混合物に塩化アンモニウム水溶液と酢酸エチルを加え、次いで、アンモニア水溶液をｐＨ約８まで加え、抽出した。有機層を飽和食塩水で洗浄し、それを無水硫酸マグネシウムで乾燥し、その溶媒を減圧下留去した。残渣をＮＨシリカゲルカラムクロマトグラフィー（ヘプタン：酢酸エチル＝３：２）で精製し、次いで、tert－ブチルメチルエーテルとヘプタンの混合溶媒を用いて結晶化し、標記化合物（５．３２ｇ）を得た。
1H－NMR  Spectrum  (CDCl3)δ(ppm)：4.07(2H,s),  5.37(4H,brs),　6.25(1H,s),  6.71(1H,dd,J=4.8,7.7Hz),  6.79－6.81(1H,m),  6.89(1H,ddd,J=0.8,5.0,7.0Hz),　7.30(2H,d,J=7.9Hz),  7.44(2H,d,J=8.1Hz),　7.58(1H,ddd,J=2.0,7.1,8.4Hz),  7.70(1H,dd,J=1.8,7.7Hz),  8.14(1H,dd,J=1.8,4.9Hz),  8.17－8.18(1H,m).
Figure JPOXMLDOC01-appb-C000033
 To a mixture of zinc chloride (8.82 g) and tetrahydrofuran (130 mL) at 0 ° C., 3-ethynyl-pyridin-2-ylamine (3) described in Production Example 1-2-3 of WO 07/052615 0.004 g, purity 98%) and 4- (pyridin-2-yloxymethyl) -phenyl-acetohydroxymoyl chloride described in Preparation Example 1-2-5 (17.4 g, purity 94%) were added. The reaction mixture was brought to room temperature, and triethylamine (9.02 mL) was added dropwise while maintaining the internal temperature at 28 ° C. or lower using a water bath. The reaction mixture was stirred at room temperature for 20 minutes and then stirred at 35 ° C. for 1 hour. The reaction mixture was brought to room temperature, an aqueous ammonium chloride solution and ethyl acetate were added to the reaction mixture, and then an aqueous ammonia solution was added to pH about 8 and extracted. The organic layer was washed with saturated brine, dried over anhydrous magnesium sulfate, and the solvent was evaporated under reduced pressure. The residue was purified by NH silica gel column chromatography (heptane: ethyl acetate = 3: 2), and then crystallized using a mixed solvent of tert-butyl methyl ether and heptane to obtain the title compound (5.32 g).
1 H-NMR Spectrum (CDCl 3 ) δ (ppm): 4.07 (2H, s), 5.37 (4H, brs), 6.25 (1H, s), 6.71 (1H, dd, J = 4.8, 7.7 Hz), 6.79 -6.81 (1H, m), 6.89 (1H, ddd, J = 0.8,5.0,7.0Hz), 7.30 (2H, d, J = 7.9Hz), 7.44 (2H, d, J = 8.1Hz), 7.58 ( 1H, ddd, J = 2.0,7.1,8.4Hz), 7.70 (1H, dd, J = 1.8,7.7Hz), 8.14 (1H, dd, J = 1.8,4.9Hz), 8.17-8.18 (1H, m) .
　参考例１の別法１では、出発物質４－（ピリジン－２－イルオキシメチル）－フェニル－アセトヒドロキシモイル　クロリドは以下の方法で合成した。 In Alternative Method 1 of Reference Example 1, the starting material 4- (pyridin-2-yloxymethyl) -phenyl-acetohydroxymoyl chloride was synthesized by the following method.
［製造例１－３－１］メチル　３－（４－（ピリジン－２－イルオキシメチル）－フェニル）－オキシラン－２－カルボキシレート [Production Example 1-3-1] Methyl 3- (4- (Pyridin-2-yloxymethyl) -phenyl) -oxirane-2-carboxylate
Figure JPOXMLDOC01-appb-C000034
 　製造例１－１－２に記載の４－（ピリジン－２－イルオキシメチル）－ベンズアルデヒド（２４．８ｇ）とテトラヒドロフラン（１６０ｍＬ）の混合物に－１５℃でメチル　クロロアセテート（１０．２ｍＬ）を加え、次いで、同温でソジウム　メトキシド（２３．７ｍＬ、２８％メタノール溶液）を加えた。反応混合物を０℃で１時間撹拌し、次いで、室温で２時間攪拌した。酢酸（６ｍＬ）を含む氷水（８００ｍＬ）に反応混合物を加え、反応混合物を室温とした。反応混合物に酢酸エチルを加え抽出し、次いで有機層を分離し、飽和食塩水で洗浄し、無水硫酸マグネシウムで乾燥した。その溶媒を減圧下留去し、標記化合物（３０．２ｇ）を得た。
1H－NMR  Spectrum  (CDCl3)δ(ppm)：3.51(1H,d,J=1.8Hz),  3.83(3H,s),　4.11(1H,d,J=1.8Hz),  5.38(2H,s),　6.81(1H,td,J=0.9,8.4Hz),　6.89(1H,ddd,J=0.9,5.1,7.1Hz),  7.29－7.31(2H,m),  7.47(2H,d,J=8.2Hz),　7.59(1H,ddd,J=2.0,7.1,8.4Hz),  8.17(1H,ddd,J=0.8,2.0,5.1Hz).
Figure JPOXMLDOC01-appb-C000034
 To a mixture of 4- (pyridin-2-yloxymethyl) -benzaldehyde (24.8 g) and tetrahydrofuran (160 mL) described in Preparation Example 1-1-2, methyl chloroacetate (10.2 mL) was added at −15 ° C. Then, sodium methoxide (23.7 mL, 28% methanol solution) was added at the same temperature. The reaction mixture was stirred at 0 ° C. for 1 hour and then at room temperature for 2 hours. The reaction mixture was added to ice water (800 mL) containing acetic acid (6 mL), and the reaction mixture was allowed to reach room temperature. Ethyl acetate was added to the reaction mixture for extraction, and then the organic layer was separated, washed with saturated brine, and dried over anhydrous magnesium sulfate. The solvent was distilled off under reduced pressure to obtain the title compound (30.2 g).
1 H-NMR Spectrum (CDCl 3 ) δ (ppm): 3.51 (1H, d, J = 1.8Hz), 3.83 (3H, s), 4.11 (1H, d, J = 1.8Hz), 5.38 (2H, s ), 6.81 (1H, td, J = 0.9,8.4Hz), 6.89 (1H, ddd, J = 0.9,5.1,7.1Hz), 7.29-7.31 (2H, m), 7.47 (2H, d, J = 8.2 Hz), 7.59 (1H, ddd, J = 2.0, 7.1, 8.4 Hz), 8.17 (1 H, ddd, J = 0.8, 2.0, 5.1 Hz).
［製造例１－３－２］ソジウム　３－（４－（ピリジン－２－イルオキシメチル）－フェニル）－オキシラン－２－カルボキシレート [Production Example 1-3-2] Sodium 3- (4- (pyridin-2-yloxymethyl) -phenyl) -oxirane-2-carboxylate
Figure JPOXMLDOC01-appb-C000035
 　製造例１－３－１に記載のメチル　３－（４－（ピリジン－２－イルオキシメチル）－フェニル）－オキシラン－２－カルボキシレート（１９．９ｇ）とエタノール（３００ｍＬ）の混合物に０℃でソジウム　メトキシド（１４．２ｍＬ、２８％メタノール溶液）、水（１．３ｍＬ）、テトラヒドロフラン（１００ｍＬ）を順次加え、室温で１時間撹拌した。反応混合物にジエチルエーテル（２００ｍＬ）を加え、析出した固体をろ取し、標記化合物（１４．３ｇ）を得た。
1H－NMR  Spectrum  (CD3OD)δ(ppm)：3.31(1H,d,J=1.8Hz),  3.88(1H,d,J=1.8Hz),　5.33(2H,s),  6.84(1H,td,J=0.9,8.2Hz),  6.94(1H,ddd,J=0.9,5.1,7.1Hz),　7.29－7.31(2H,m),  7.42(2H,d,J=8.2Hz),　7.68(1H,ddd,J=2.0,7.1,8.4Hz),  8.12(1H,ddd,J=0.7,2.0,5.1Hz).
Figure JPOXMLDOC01-appb-C000035
 To a mixture of methyl 3- (4- (pyridin-2-yloxymethyl) -phenyl) -oxirane-2-carboxylate (19.9 g) and ethanol (300 mL) described in Preparation Example 1-3-1 at 0 ° C. Sodium methoxide (14.2 mL, 28% methanol solution), water (1.3 mL) and tetrahydrofuran (100 mL) were sequentially added, and the mixture was stirred at room temperature for 1 hour. Diethyl ether (200 mL) was added to the reaction mixture, and the precipitated solid was collected by filtration to obtain the title compound (14.3 g).
1 H-NMR Spectrum (CD 3 OD) δ (ppm): 3.31 (1H, d, J = 1.8 Hz), 3.88 (1H, d, J = 1.8 Hz), 5.33 (2H, s), 6.84 (1H, td, J = 0.9,8.2Hz), 6.94 (1H, ddd, J = 0.9,5.1,7.1Hz), 7.29-7.31 (2H, m), 7.42 (2H, d, J = 8.2Hz), 7.68 (1H , ddd, J = 2.0,7.1,8.4Hz), 8.12 (1H, ddd, J = 0.7,2.0,5.1Hz).
［製造例１－３－３］４－（ピリジン－２－イルオキシメチル）－フェニル－アセタルデヒド [Production Example 1-3-3] 4- (Pyridin-2-yloxymethyl) -phenyl-acetaldehydride
Figure JPOXMLDOC01-appb-C000036
 　製造例１－３－２に記載のソジウム　３－（４－（ピリジン－２－イルオキシメチル）－フェニル）－オキシラン－２－カルボキシレート（９．９５ｇ）、トルエン（２００ｍＬ）、水（１２０ｍＬ）、酢酸（１６ｍＬ）の混合物を７３℃で９０分間撹拌した。反応混合物を室温とし、反応混合物に酢酸エチルを加え抽出し、次いで有機層を分離し、飽和食塩水で洗浄し、無水硫酸マグネシウムで乾燥した。その溶媒を減圧下留去し、標記化合物（６．８２ｇ）を得た。
1H－NMR  Spectrum  (CDCl3)δ(ppm)：3.70(2H,d,J=2.2Hz),  5.38(2H,s),　6.81(1H,td,J=0.8,8.2Hz),　6.89(1H,ddd,J=0.9,5.1,7.1Hz),  7.24(2H,d,J=8.1),　7.48(2H,d,J=8.1Hz),  7.59(1H,ddd,J=2.0,7.1,8.4Hz),　8.18(1H,ddd,J=0.6,2.0,5.0Hz),  9.75(1H,t,J=2.4).
Figure JPOXMLDOC01-appb-C000036
 Sodium 3- (4- (pyridin-2-yloxymethyl) -phenyl) -oxirane-2-carboxylate (9.95 g), toluene (200 mL), water (120 mL) described in Preparation Example 1-3-2 , A mixture of acetic acid (16 mL) was stirred at 73 ° C. for 90 minutes. The reaction mixture was brought to room temperature, ethyl acetate was added to the reaction mixture for extraction, then the organic layer was separated, washed with saturated brine, and dried over anhydrous magnesium sulfate. The solvent was distilled off under reduced pressure to obtain the title compound (6.82 g).
1 H-NMR Spectrum (CDCl 3 ) δ (ppm): 3.70 (2H, d, J = 2.2Hz), 5.38 (2H, s), 6.81 (1H, td, J = 0.8,8.2Hz), 6.89 (1H , ddd, J = 0.9,5.1,7.1Hz), 7.24 (2H, d, J = 8.1), 7.48 (2H, d, J = 8.1Hz), 7.59 (1H, ddd, J = 2.0,7.1,8.4Hz ), 8.18 (1H, ddd, J = 0.6,2.0,5.0Hz), 9.75 (1H, t, J = 2.4).
［製造例１－３－４］４－（ピリジン－２－イルオキシメチル）－フェニル－アセタルデヒド　オキシム（Ｅ／Ｚ混合物） [Production Example 1-3-4] 4- (Pyridin-2-yloxymethyl) -phenyl-acetaldehydrate oxime (E / Z mixture)
Figure JPOXMLDOC01-appb-C000037
 　ヒドロキシルアミン硫酸塩（１９．７ｇ）と水（２５０ｍＬ）の混合物に、０℃で１Ｎ水酸化ナトリウム水溶液（２４０ｍＬ）を加え、同温で１５分間攪拌した。次いで、反応混合物に、同温で、製造例１－３－３に記載の（４－（ピリジン－２－イルオキシメチル）－フェニル）－アセタルデヒド（２７．３ｇ）とメタノール（２５０ｍＬ）の混合物を滴下し、室温で終夜攪拌した。析出した固体をろ取し、標記化合物（２０．３ｇ）をＥ体とＺ体の混合物として得た。
1H－NMR  Spectrum  (CDCl3)δ(ppm)：3.54(2H,d,J=6.2Hz),　3.74(2H,d,J=5.3Hz),  5.36(2H+2H,s),  6.79－6.81(1H+1H,m),  6.87－6.90(1H+2H,m),  7.22－7.24(2H+2H,m),  7.42－7.44(2H+2H,m),  7.53(1H,t,J=6.3 Hz),  7.56－7.61(1H+1H,m),  8.17－8.18(1H+1H,m)(underbar=E  or  Z).
Figure JPOXMLDOC01-appb-C000037
 To a mixture of hydroxylamine sulfate (19.7 g) and water (250 mL) was added 1N aqueous sodium hydroxide solution (240 mL) at 0 ° C., and the mixture was stirred at the same temperature for 15 min. Next, the reaction mixture was mixed with a mixture of (4- (pyridin-2-yloxymethyl) -phenyl) -acetaldehydride (27.3 g) and methanol (250 mL) described in Preparation Example 1-3-3 at the same temperature. The solution was added dropwise and stirred overnight at room temperature. The precipitated solid was collected by filtration to obtain the title compound (20.3 g) as a mixture of E-form and Z-form.
1 H-NMR Spectrum (CDCl 3 ) δ (ppm): 3.54 ( 2H , d, J = 6.2Hz), 3.74 (2H, d, J = 5.3Hz), 5.36 ( 2H + 2H, s), 6.79-6.81 ( 1H + 1H, m), 6.87-6.90 ( 1H + 2H, m), 7.22-7.24 ( 2H + 2H, m), 7.42-7.44 ( 2H + 2H, m), 7.53 ( 1H , t, J = 6.3 Hz), 7.56-7.61 ( 1H + 1H, m), 8.17-8.18 ( 1H + 1H, m) (underbar = E or Z).
［製造例１－３－５］４－（ピリジン－２－イルオキシメチル）－フェニル－アセトヒドロキシモイル　クロリド [Production Example 1-3-5] 4- (Pyridin-2-yloxymethyl) -phenyl-acetohydroxymoyl chloride
Figure JPOXMLDOC01-appb-C000038
 　製造例１－３－４に記載の４－（ピリジン－２－イルオキシメチル）－フェニル－アセタルデヒド　オキシム（Ｅ／Ｚ混合物）（１３２ｍｇ）とＮ,Ｎ-ジメチルホルムアミド（２ｍＬ）の混合物に、室温でＮ－クロロスクシニミド（７２．８ｍｇ）を加えた。次いで、同温で、反応混合物に塩酸ガスを吹き込み、同温で９０分間撹拌した。反応混合物に酢酸エチルと水を加え抽出し、次いで有機層を分離し、飽和食塩水で洗浄し、無水硫酸マグネシウムで乾燥した。その溶媒を減圧下留去し、得られた残渣をジエチルエーテルとヘプタンの混合溶媒で洗浄し、標記化合物（１２３ｍｇ）を得た。
1H－NMR  Spectrum  (CDCl3)δ(ppm)：3.81(2H,s),  5.36(2H,s),　6.81(1H,d,J=8.2Hz),  6.88－6.91　(1H,m),  7.28(2H,d,J=8.1),　7.43(2H,d,J=8.1Hz),  7.57－7.62(1H,m),  8.17－8.19(1H,m).
Figure JPOXMLDOC01-appb-C000038
 To a mixture of 4- (pyridin-2-yloxymethyl) -phenyl-acetaldehyde oxime (E / Z mixture) (132 mg) and N, N-dimethylformamide (2 mL) described in Preparation Example 1-3-4 at room temperature N-chlorosuccinimide (72.8 mg) was added. Next, hydrochloric acid gas was blown into the reaction mixture at the same temperature, and the mixture was stirred at the same temperature for 90 minutes. The reaction mixture was extracted with ethyl acetate and water, and then the organic layer was separated, washed with saturated brine, and dried over anhydrous magnesium sulfate. The solvent was distilled off under reduced pressure, and the resulting residue was washed with a mixed solvent of diethyl ether and heptane to obtain the title compound (123 mg).
1 H-NMR Spectrum (CDCl 3 ) δ (ppm): 3.81 (2H, s), 5.36 (2H, s), 6.81 (1H, d, J = 8.2 Hz), 6.88-6.91 (1H, m), 7.28 (2H, d, J = 8.1), 7.43 (2H, d, J = 8.1Hz), 7.57-7.62 (1H, m), 8.17-8.19 (1H, m).
［参考例１の別法２］３－（３－（４－（ピリジン－２－イルオキシメチル）－ベンジル）－イソキサゾール－５－イル）－ピリジン－２－イルアミン [Alternative Method 2 of Reference Example 1] 3- (3- (4- (Pyridin-2-yloxymethyl) -benzyl) -isoxazol-5-yl) -pyridin-2-ylamine
Figure JPOXMLDOC01-appb-C000039
 　製造例１－４－２に記載のジ－tert－ブチル　（３－(３－（４－（（ピリジン－２－イルオキシ）メチル）ベンジル)イソキサゾール－５－イル）ピリジン－２－イル）イミドジカルボネート（１１．８ｇ、純度約７０％）、ジクロロメタン（１２０ｍＬ）の溶液に０℃でトリフルオロ酢酸（４０ｍＬ）を加えた。室温で１４時間撹拌した。反応溶液に２０℃以下で飽和重曹水を加え酢酸エチルで抽出し、ついでＮＨ－シリカゲルカラムクロマトグラフィー（ヘプタン：酢酸エチル＝１：１）で精製した。溶媒を減圧濃縮し、得られた残渣にtert－ブチルメチルエーテルを加え固体をろ取し、標記化合物（７．２９ｇ）を得た。
1H－NMR  Spectrum  (DMSO－d6)δ(ppm)：4.04(2H,s),  5.32(2H,s),　6.26(2H,brs),  6.69(1H,dd,J=4.8,8.0Hz),  6.81(1H,s),　6.83－6.87(1H,m),  6.97－7.00(1H,m),  7.33(2H,d,J=8.0Hz),　7.40(2H,d,J=8.0Hz),  7.69－7.74(1H,m),  7.87(1H,dd,J=2.0,7.6Hz),  8.08(1H,dd,J=2.0,7.6Hz),  8.15－8.17(1H,m).
Figure JPOXMLDOC01-appb-C000039
 Di-tert-butyl (3- (3- (4-((pyridin-2-yloxy) methyl) benzyl) isoxazol-5-yl) pyridin-2-yl) imide dical described in Preparation Example 1-4-2 Trifluoroacetic acid (40 mL) was added to a solution of Bonate (11.8 g, purity about 70%) and dichloromethane (120 mL) at 0 ° C. Stir at room temperature for 14 hours. Saturated aqueous sodium hydrogen carbonate was added to the reaction solution at 20 ° C. or lower, and the mixture was extracted with ethyl acetate. The solvent was concentrated under reduced pressure, tert-butyl methyl ether was added to the resulting residue, and the solid was collected by filtration to obtain the title compound (7.29 g).
1 H-NMR Spectrum (DMSO-d6) δ (ppm): 4.04 (2H, s), 5.32 (2H, s), 6.26 (2H, brs), 6.69 (1H, dd, J = 4.8, 8.0 Hz), 6.81 (1H, s), 6.83-6.87 (1H, m), 6.97-7.00 (1H, m), 7.33 (2H, d, J = 8.0Hz), 7.40 (2H, d, J = 8.0Hz), 7.69 -7.74 (1H, m), 7.87 (1H, dd, J = 2.0,7.6Hz), 8.08 (1H, dd, J = 2.0,7.6Hz), 8.15-8.17 (1H, m).
　出発物質ジ－tert－ブチル　（３－(３－（４－（（ピリジン－２－イルオキシ）メチル）ベンジル)イソキサゾール－５－イル）ピリジン－２－イル）イミドジカルボネートは以下の方法で合成した。 Di-tert-butyl starting material (3- (3- (4-((pyridin-2-yloxy) methyl) benzyl) isoxazol-5-yl) pyridin-2-yl) imide dicarbonate was synthesized by the following method. .
［製造例１－４－１］　ジ－tert－ブチル　（３－エチニルピリジン－２－イル）イミドジカルボネート [Production Example 1-4-1] Di-tert-butyl (3-ethynylpyridin-2-yl) imide dicarbonate
Figure JPOXMLDOC01-appb-C000040
 　国際公開第０７/０５２６１５号公報の製造例１－２－３に記載の３－エチニル－ピリジン－２－イルアミン（６．３４ｇ）、ジ－tert－ブチル　ジカルボネート（５８．５ｇ）、トリエチルアミン（２７．１ｇ）、４－ジメチルアミノピリジン（６５５ｍｇ）、テトラヒドロフラン（２５４ｍＬ）を室温で１８時間撹拌した。反応溶液にシリカゲルを加え溶媒を減圧濃縮した。得られたシリカゲルをシリカゲルクロマトグラフィー（ヘプタン：酢酸エチル＝３：１）で精製し、標記化合物（１５ｇ）を白色個体として得た。
1H－NMR  Spectrum  (DMSO－d6)δ(ppm)：1.32(18H,s),　4.59(1H,s),  7.39－7.44(1H,m),  7.99－8.03(1H,m),  8.46－8.48(1H,m).
Figure JPOXMLDOC01-appb-C000040
 3-Ethynyl-pyridin-2-ylamine (6.34 g), di-tert-butyl dicarbonate (58.5 g), and triethylamine (27.27) described in Preparation Example 1-2-3 of WO 07/052615. 1 g), 4-dimethylaminopyridine (655 mg), and tetrahydrofuran (254 mL) were stirred at room temperature for 18 hours. Silica gel was added to the reaction solution, and the solvent was concentrated under reduced pressure. The obtained silica gel was purified by silica gel chromatography (heptane: ethyl acetate = 3: 1) to obtain the title compound (15 g) as a white solid.
1 H-NMR Spectrum (DMSO-d6) δ (ppm): 1.32 (18H, s), 4.59 (1H, s), 7.39-7.44 (1H, m), 7.99-8.03 (1H, m), 8.46-8.48 (1H, m).
［製造例１－４－２］　ジ－tert－ブチル　（３－(３－（４－（（ピリジン－２－イルオキシ）メチル）ベンジル)イソキサゾール－５－イル）ピリジン－２－イル）イミドジカルボネート [Production Example 1-4-2] Di-tert-butyl (3- (3- (4-((pyridin-2-yloxy) methyl) benzyl) isoxazol-5-yl) pyridin-2-yl) imide dicarbonate
Figure JPOXMLDOC01-appb-C000041
 　製造例１－４－１に記載のジ－tert－ブチル　（３－エチニルピリジン－２－イル）イミドジカルボネート（１２ｇ）、製造例１－１－４に記載の２－（４－（２－ニトロ－エチル）－ベンジルオキシ）ピリジン（１９．４ｇ）、４－ジメチルアミノピリジン（２３０ｍｇ）、テトラヒドロフラン（２００ｍＬ）の溶液に室温撹拌下にジ－tert－ブチル　ジカルボネート（２８．８ｇ）を４回に分けて８時間かけて加えた。加え終わった後室温でさらに２２時間撹拌した。反応溶液にシリカゲルを加え溶媒を減圧濃縮した。得られたシリカゲルをシリカゲルクロマトグラフィー（ヘプタン：酢酸エチル＝３：１ついで２:１）で精製し、標記化合物を含む油状物（１１．８ｇ、目的物を約７０％含む）を得た。
Figure JPOXMLDOC01-appb-C000041
 Di-tert-butyl (3-ethynylpyridin-2-yl) imide dicarbonate (12 g) described in Preparation Example 1-4-1 and 2- (4- (2- (2- Nitro-ethyl) -benzyloxy) pyridine (19.4 g), 4-dimethylaminopyridine (230 mg) and tetrahydrofuran (200 mL) were stirred at room temperature with di-tert-butyl dicarbonate (28.8 g) in four portions. Added in portions over 8 hours. After the addition was complete, the mixture was further stirred at room temperature for 22 hours. Silica gel was added to the reaction solution, and the solvent was concentrated under reduced pressure. The resulting silica gel was purified by silica gel chromatography (heptane: ethyl acetate = 3: 1 and then 2: 1) to obtain an oily substance (11.8 g, containing about 70% of the desired product) containing the title compound.
 製造例１－４－２のジ－tert－ブチル　（３－(３－（４－（（ピリジン－２－イルオキシ）メチル）ベンジル)イソキサゾール－５－イル）ピリジン－２－イル）イミドジカルボネートは、下記の別法１又は２でも合成できる。 Di-tert-butyl (3- (3- (4-((pyridin-2-yloxy) methyl) benzyl) isoxazol-5-yl) pyridin-2-yl) imide dicarbonate of Production Example 1-4-2 is Alternatively, it can be synthesized by the following Alternative 1 or 2.
［製造例１－５－１］　ジ－tert－ブチル　（３－(３－（４－（（ピリジン－２－イルオキシ）メチル）ベンジル)イソキサゾール－５－イル）ピリジン－２－イル）イミドジカルボネート（製造例１－４－２の別法１） [Production Example 1-5-1] Di-tert-butyl (3- (3- (4-((pyridin-2-yloxy) methyl) benzyl) isoxazol-5-yl) pyridin-2-yl) imide dicarbonate (Alternative Method 1 of Production Example 1-4-2)
Figure JPOXMLDOC01-appb-C000042
 　製造例１－４－１に記載のジ－tert－ブチル　（３－エチニルピリジン－２－イル）イミドジカルボネート（２.０ｇ）、製造例１－１－４に記載の２－（４－（２－ニトロ－エチル）－ベンジルオキシ）ピリジン（２．４４ｇ）、トリエチルアミン（０．０８６ｕＬ）、テトラヒドロフラン（２０ｍＬ）の溶液に５０℃撹拌下にフェニルイソシアネート（２．８ｍＬ）を４回に分けて５．５時間かけて加えた。加え終わった後５０℃でさらに２時間撹拌した。反応溶液にＮＨ－シリカゲルを加え溶媒を減圧濃縮した。ＮＨ－シリカゲルに吸着した粗体をＮＨ－シリカゲルクロマトグラフィー（ヘプタン：酢酸エチル＝３：１）で精製した。得られた溶液を減圧濃縮しシリカゲルクロマトグラフィー（ヘプタン：酢酸エチル＝３：１ついで２：１）で精製し、標記化合物（２．２ｇ）を油状物として得た。
1H－NMR  Spectrum  (DMSO－d6)δ(ppm)：1.18(18H,s),　4.07(2H,s),  5.32(2H,s),　6.58(1H,s),  6.83－6.86(1H,m),  6.96－7.01(1H,m),  7.29(2H,d,J=8.0Hz),　7.40(2H,d,J=8.0Hz),  7.58(1H,dd,J=4.8,7.6Hz),  7.69－7.74(1H,m),  8.15－8.18(1H,m),  8.34(1H,dd,J=2.0,7.6Hz),  8.59(1H,dd,J=2.0,5.2Hz).
Figure JPOXMLDOC01-appb-C000042
 Di-tert-butyl (3-ethynylpyridin-2-yl) imide dicarbonate (2.0 g) described in Preparation Example 1-4-1 and 2- (4- ( 2-Nitro-ethyl) -benzyloxy) pyridine (2.44 g), triethylamine (0.086 uL), tetrahydrofuran (20 mL) in a solution of phenyl isocyanate (2.8 mL) in 4 portions while stirring at 50 ° C. Added over 5 hours. After the addition was completed, the mixture was further stirred at 50 ° C. for 2 hours. NH-silica gel was added to the reaction solution, and the solvent was concentrated under reduced pressure. The crude product adsorbed on NH-silica gel was purified by NH-silica gel chromatography (heptane: ethyl acetate = 3: 1). The resulting solution was concentrated under reduced pressure and purified by silica gel chromatography (heptane: ethyl acetate = 3: 1 then 2: 1) to give the title compound (2.2 g) as an oil.
1 H-NMR Spectrum (DMSO-d6) δ (ppm): 1.18 (18H, s), 4.07 (2H, s), 5.32 (2H, s), 6.58 (1H, s), 6.83-6.86 (1H, m ), 6.96-7.01 (1H, m), 7.29 (2H, d, J = 8.0Hz), 7.40 (2H, d, J = 8.0Hz), 7.58 (1H, dd, J = 4.8,7.6Hz), 7.69 -7.74 (1H, m), 8.15-8.18 (1H, m), 8.34 (1H, dd, J = 2.0,7.6Hz), 8.59 (1H, dd, J = 2.0,5.2Hz).
［製造例１－６－１］　ジ－tert－ブチル　（３－(３－（４－（（ピリジン－２－イルオキシ）メチル）ベンジル)イソキサゾール－５－イル）ピリジン－２－イル）イミドジカルボネート（製造例１－４－２の別法２） [Production Example 1-6-1] Di-tert-butyl (3- (3- (4-((pyridin-2-yloxy) methyl) benzyl) isoxazol-5-yl) pyridin-2-yl) imide dicarbonate (Alternative Method 2 of Production Example 1-4-2)
Figure JPOXMLDOC01-appb-C000043
 　製造例１－６－２に記載の４－メチレン－２－オキソ－４Ｈ－ピリド［２，３－ｄ］［１，３］オキサジン－１－カルボキシリック　アシッド　ｔｅｒｔ－ブチル　エステル（１．４８ｇ）、製造例１－１－４に記載の２－（４－（２－ニトロ－エチル）－ベンジルオキシ）ピリジン（２．９ｇ）、ジ－tert－ブチル　ジカルボネート（６．１４ｇ）、４－ジメチルアミノピリジン（６８．６ｍｇ）、テトラヒドロフラン（５０ｍＬ）を室温で２時間撹拌した。反応溶液にシリカゲルを加え溶媒を減圧濃縮した。シリカゲルに吸着した粗体をシリカゲルクロマトグラフィー（ヘプタン：酢酸エチル＝３：１ついで１：１ついで１：２）で精製し標記化合物（２．１ｇ）を油状物として得た。
1H－NMR  Spectrum  (DMSO－d6)δ(ppm)：1.18(18H,s),　4.07(2H,s),  5.32(2H,s),　6.58(1H,s),  6.83－6.86(1H,m),  6.96－7.01(1H,m),  7.29(2H,d,J=8.0Hz),　7.40(2H,d,J=8.0Hz),  7.58(1H,dd,J=4.8,7.6Hz),  7.69－7.74(1H,m),  8.15－8.18(1H,m),  8.34(1H,dd,J=2.0,7.6Hz),  8.59(1H,dd,J=2.0,5.2Hz).
Figure JPOXMLDOC01-appb-C000043
 4-methylene-2-oxo-4H-pyrido [2,3-d] [1,3] oxazine-1-carboxylic acid tert-butyl ester (1.48 g) described in Preparation Example 1-6-2, 2- (4- (2-Nitro-ethyl) -benzyloxy) pyridine (2.9 g), di-tert-butyl dicarbonate (6.14 g), 4-dimethylaminopyridine described in Preparation Example 1-1-4 (68.6 mg) and tetrahydrofuran (50 mL) were stirred at room temperature for 2 hours. Silica gel was added to the reaction solution, and the solvent was concentrated under reduced pressure. The crude product adsorbed on silica gel was purified by silica gel chromatography (heptane: ethyl acetate = 3: 1 then 1: 1 then 1: 2) to obtain the title compound (2.1 g) as an oil.
1 H-NMR Spectrum (DMSO-d6) δ (ppm): 1.18 (18H, s), 4.07 (2H, s), 5.32 (2H, s), 6.58 (1H, s), 6.83-6.86 (1H, m ), 6.96-7.01 (1H, m), 7.29 (2H, d, J = 8.0Hz), 7.40 (2H, d, J = 8.0Hz), 7.58 (1H, dd, J = 4.8,7.6Hz), 7.69 -7.74 (1H, m), 8.15-8.18 (1H, m), 8.34 (1H, dd, J = 2.0,7.6Hz), 8.59 (1H, dd, J = 2.0,5.2Hz).
　出発物質４－メチレン－２－オキソ－４Ｈ－ピリド［２，３－ｄ］［１，３］オキサジン－１－カルボキシリック　アシッド　ｔｅｒｔ－ブチル　エステルは、以下の方法で合成した。 Starting material 4-methylene-2-oxo-4H-pyrido [2,3-d] [1,3] oxazine-1-carboxyl acid acid tert-butyl ester was synthesized by the following method.
［製造例１－６－２］４－メチレン－２－オキソ－４Ｈ－ピリド［２，３－ｄ］［１，３］オキサジン－１－カルボキシリック　アシッド　ｔｅｒｔ－ブチル　エステル [Production Example 1-6-2] 4-methylene-2-oxo-4H-pyrido [2,3-d] [1,3] oxazine-1-carboxylic acid acid tert-butyl ester
Figure JPOXMLDOC01-appb-C000044
 　１－（２－アミノ－ピリジン－３－イル）－エタノン（９９０ｍｇ）、ジ－tert－ブチル　ジカルボネート（７．９２ｇ）、４－ジメチルアミノピリジン（８８．８ｍｇ）、トリエチルアミン（４．９５ｍＬ）、テトラヒドロフラン（１６．５ｍｌ）を室温で２４時間撹拌した。反応溶液にシリカゲルを加え溶媒を減圧濃縮した。シリカゲルに吸着した粗体をシリカゲルクロマトグラフィー（ヘプタン：酢酸エチル＝２：１）で精製し、標記化合物（１．４８ｇ）を油状物として得た。
1H－NMR  Spectrum  (DMSO－d6)δ(ppm)：1.56(9H,s),  5.01(1H,d,J=3.6Hz),　5.45(1H,d,J=3.6Hz),  7.28(1H,dd,J=4.8,8.0Hz),  8.25(1H,dd,J=1.6,8.0Hz),  8.36(1H,dd,J=1.6,4.8Hz).
Figure JPOXMLDOC01-appb-C000044
 1- (2-Amino-pyridin-3-yl) -ethanone (990 mg), di-tert-butyl dicarbonate (7.92 g), 4-dimethylaminopyridine (88.8 mg), triethylamine (4.95 mL), tetrahydrofuran (16.5 ml) was stirred at room temperature for 24 hours. Silica gel was added to the reaction solution, and the solvent was concentrated under reduced pressure. The crude product adsorbed on silica gel was purified by silica gel chromatography (heptane: ethyl acetate = 2: 1) to obtain the title compound (1.48 g) as an oil.
1 H-NMR Spectrum (DMSO-d6) δ (ppm): 1.56 (9H, s), 5.01 (1H, d, J = 3.6Hz), 5.45 (1H, d, J = 3.6Hz), 7.28 (1H, dd, J = 4.8,8.0Hz), 8.25 (1H, dd, J = 1.6,8.0Hz), 8.36 (1H, dd, J = 1.6,4.8Hz).
［参考例１の別法３］３－（３－（４－（ピリジン－２－イルオキシメチル）－ベンジル）－イソキサゾール－５－イル）－ピリジン－２－イルアミン [Alternative Method 3 of Reference Example 1] 3- (3- (4- (Pyridin-2-yloxymethyl) -benzyl) -isoxazol-5-yl) -pyridin-2-ylamine
Figure JPOXMLDOC01-appb-C000045
 　窒素雰囲気下、製造例１－８－２に記載の２－（４－（５－ヨード－イソキサゾール－３－イルメチル）－ベンジルオキシ）－ピリジン(２００ｍｇ)、製造例１－７－２に記載の２－tert－ブトキシカルボニルアミノ－３－ピリジンボロン酸（１３４ｍｇ）、炭酸ナトリウム(８２ｍｇ)、テトラキス（トリフェニルホスフィン）パラジウム（５９ｍｇ）、１，２－ジメトキシエタン(６ｍＬ)及び水（１ｍＬ）の混合物を８０℃で２時間撹拌した。その混合物を室温まで冷却し、酢酸エチルと水を加えた。その有機層を分離し、水と飽和食塩水で洗浄し、無水硫酸マグネシウムで乾燥後、ろ過した。そのろ液をシリカゲルに吸着した後、シリカゲルカラムクロマトグラフィー（ヘプタン：酢酸エチル＝４：１～１：１～酢酸エチル）にて精製し、標記化合物（１１６ｍｇ）を得た。
Figure JPOXMLDOC01-appb-C000045
 In a nitrogen atmosphere, 2- (4- (5-iodo-isoxazol-3-ylmethyl) -benzyloxy) -pyridine (200 mg) described in Preparation Example 1-8-2, described in Preparation Example 1-7-2 Mixture of 2-tert-butoxycarbonylamino-3-pyridineboronic acid (134 mg), sodium carbonate (82 mg), tetrakis (triphenylphosphine) palladium (59 mg), 1,2-dimethoxyethane (6 mL) and water (1 mL) Was stirred at 80 ° C. for 2 hours. The mixture was cooled to room temperature and ethyl acetate and water were added. The organic layer was separated, washed with water and saturated brine, dried over anhydrous magnesium sulfate, and filtered. The filtrate was adsorbed onto silica gel and purified by silica gel column chromatography (heptane: ethyl acetate = 4: 1 to 1: 1 to ethyl acetate) to obtain the title compound (116 mg).
　出発物質２－tert－ブトキシカルボニルアミノ－３－ピリジンボロン酸は以下の方法で合成した。 The starting material 2-tert-butoxycarbonylamino-3-pyridineboronic acid was synthesized by the following method.
［製造例１－７－１］ピリジン－２－イル－カルバミック　アシッド　tert-ブチル　エステル [Production Example 1-7-1] Pyridin-2-yl-carbamic acid tert-butyl ester
Figure JPOXMLDOC01-appb-C000046
 　tert－ブチルアルコール（６５０ｍＬ）とジ－tert－ブチルカーボネート（２４ｇ）の溶液にゆっくり２－アミノピリジン（９．４ｇ）を加えた。その混合物を室温で２４時間撹拌した。その反応溶液を減圧濃縮し、その残渣をシリカゲルカラムクロマトグラフィー（ヘプタン：酢酸エチル＝１：１）で精製し、標記化合物（１８ｇ）を得た。
1H－NMR  Spectrum  (DMSO－d6)δ(ppm)：1.47(9H,s),  6.99－7.03(1H,m),  7.70－7.74(1H,m),  7.77－7.80(1H,m),  8.23－8.24(1H,m),  9.72(1H,brs).
Figure JPOXMLDOC01-appb-C000046
 To a solution of tert-butyl alcohol (650 mL) and di-tert-butyl carbonate (24 g) was slowly added 2-aminopyridine (9.4 g). The mixture was stirred at room temperature for 24 hours. The reaction solution was concentrated under reduced pressure, and the residue was purified by silica gel column chromatography (heptane: ethyl acetate = 1: 1) to obtain the title compound (18 g).
1 H-NMR Spectrum (DMSO-d 6 ) δ (ppm): 1.47 (9H, s), 6.99-7.03 (1H, m), 7.70-7.74 (1H, m), 7.77-7.80 (1H, m), 8.23-8.24 (1H, m), 9.72 (1H, brs).
［製造例１－７－２］２－tert－ブトキシカルボニルアミノ－３－ピリジンボロン酸 [Production Example 1-7-2] 2-tert-butoxycarbonylamino-3-pyridineboronic acid
Figure JPOXMLDOC01-appb-C000047
 　製造例１－７－１に記載のピリジン－２－イル－カルバミック　アシッド　tert-ブチル　エステル(１６ｇ)とＮ，Ｎ，Ｎ’，Ｎ’－テトラメチルエチレンジアミン(２５ｇ)のテトラヒドロフラン溶液(４００ｍＬ)を－７０℃に冷却し、ｎ－ブチルリチウム（７８ｍＬ、２．６４Ｍ　ヘプタン溶液）を１時間で滴下し、１０分間撹拌した。その混合物を－１０℃から－６℃の間まで昇温し、その温度で２時間撹拌した。再び、その溶液を－７０℃まで冷却し、トリイソブチル　ボレート(５８ｇ)を１時間で滴下した。その混合物を０℃まで昇温した後、飽和塩化アンモニウム水溶液を加えた。生成した黄色固体にエーテルを加え、撹拌した後、固体をろ取し、エーテルと水で洗浄した。その固体を減圧下乾燥し、標記化合物（１４ｇ）を得た。
1H－NMR  Spectrum  (DMSO－d6)δ(ppm)：1.32－1.41(9H,m),  6.80－6.84(1H,m),  7.95－7.8.13(2H,m).
Figure JPOXMLDOC01-appb-C000047
 A tetrahydrofuran solution (400 mL) of pyridin-2-yl-carbamic acid tert-butyl ester (16 g) described in Production Example 1-7-1 and N, N, N ′, N′-tetramethylethylenediamine (25 g) is − After cooling to 70 ° C., n-butyllithium (78 mL, 2.64 M heptane solution) was added dropwise over 1 hour and stirred for 10 minutes. The mixture was warmed to between −10 ° C. and −6 ° C. and stirred at that temperature for 2 hours. Again, the solution was cooled to −70 ° C. and triisobutyl borate (58 g) was added dropwise over 1 hour. After the temperature of the mixture was raised to 0 ° C., a saturated aqueous ammonium chloride solution was added. Ether was added to the resulting yellow solid and stirred, and then the solid was collected by filtration and washed with ether and water. The solid was dried under reduced pressure to obtain the title compound (14 g).
1 H-NMR Spectrum (DMSO-d 6 ) δ (ppm): 1.32—1.41 (9H, m), 6.80-6.84 (1H, m), 7.95—7.8.13 (2H, m).
　参考例１の別法３における出発物質２－（４－（５－ヨード－イソキサゾール－３－イルメチル）－ベンジルオキシ）－ピリジンは以下の方法で合成した。 The starting material 2- (4- (5-iodo-isoxazol-3-ylmethyl) -benzyloxy) -pyridine in Alternative Method 3 of Reference Example 1 was synthesized by the following method.
［製造例１－８－１］２－（４－（５－トリブチルスタニル－イソキサゾール－３－イルメチル）－ベンジルオキシ）－ピリジン [Production Example 1-8-1] 2- (4- (5-Tributylstannyl-isoxazol-3-ylmethyl) -benzyloxy) -pyridine
Figure JPOXMLDOC01-appb-C000048
 　トリ－ｎ－ブチルエチニルチン（３ｇ）、製造例１－１－４に記載の２－（４－（２－ニトロ－エチル）－ベンジルオキシ）－ピリジン（４．９ｇ）、及び４－ジメチルアミノピリジン（１１６ｍｇ）のテトラヒドロフラン溶液（９０ｍＬ）に、ジ－tert－ブチルジカーボネート（７．３ｇ）のテトラヒドロフラン溶液（３０ｍＬ）を加え、室温で１５時間撹拌した。その混合物に酢酸エチルと水を加えた。その有機層を分離し、水と飽和食塩水で洗浄し、無水硫酸マグネシウムで乾燥後、ろ過した。そのろ液をシリカゲルに吸着させた後、シリカゲルカラムクロマトグラフィー（ヘプタン：酢酸エチル＝４：１）にて精製し、標記化合物（５．３ｇ）を得た。
1H－NMR  Spectrum  (DMSO－d6)δ(ppm)：0.81－0.85(9H,m),  1.08－1.12(6H,m),  1.23－1.30(6H,m),  1.46－1.54(6H,m),  4.00(2H,s),　5.30(2H,s),  6.40(1H,s),　6.83－6.86(1H,m),  6.97－7.00(1H,m),  7.25－7.26(2H,m),  7.36－7.38(2H,m),  7.69－7.74(1H,m),  8.15－8.17(1H,m).
Figure JPOXMLDOC01-appb-C000048
 Tri-n-butylethynyltin (3 g), 2- (4- (2-nitro-ethyl) -benzyloxy) -pyridine (4.9 g) described in Preparation Example 1-1-4, and 4-dimethylamino To a tetrahydrofuran solution (90 mL) of pyridine (116 mg) was added a tetrahydrofuran solution (30 mL) of di-tert-butyl dicarbonate (7.3 g), and the mixture was stirred at room temperature for 15 hours. Ethyl acetate and water were added to the mixture. The organic layer was separated, washed with water and saturated brine, dried over anhydrous magnesium sulfate, and filtered. The filtrate was adsorbed onto silica gel and purified by silica gel column chromatography (heptane: ethyl acetate = 4: 1) to obtain the title compound (5.3 g).
1 H-NMR Spectrum (DMSO-d 6 ) δ (ppm): 0.81-0.85 (9H, m), 1.08-1.12 (6H, m), 1.23-1.30 (6H, m), 1.46-1.54 (6H, m ), 4.00 (2H, s), 5.30 (2H, s), 6.40 (1H, s), 6.83-6.86 (1H, m), 6.97-7.00 (1H, m), 7.25-7.26 (2H, m), 7.36-7.38 (2H, m), 7.69-7.74 (1H, m), 8.15-8.17 (1H, m).
［製造例１－８－２］２－（４－（５－ヨード－イソキサゾール－３－イルメチル）－ベンジルオキシ）－ピリジン [Production Example 1-8-2] 2- (4- (5-Iodo-isoxazol-3-ylmethyl) -benzyloxy) -pyridine
Figure JPOXMLDOC01-appb-C000049
 　製造例１－８－１に記載の２－（４－（５－トリブチルスタニル－イソキサゾール－３－イルメチル）－ベンジルオキシ）－ピリジン(５．１ｇ)のテトラヒドロフラン溶液(１５ｍＬ)に、０℃でヨウ素（２．５ｇ）を加えた。その混合物を２０分間その温度で撹拌した。その混合物に１０％チオ硫酸ナトリウム水溶液と酢酸エチルを加えた。その有機層を分離し、飽和食塩水で洗浄し、無水硫酸マグネシウムで乾燥後、ろ過した。そのろ液を濃縮し、その残渣をシリカゲルカラムクロマトグラフィー（ヘプタン：酢酸エチル＝１０：１～４：１）にて精製し、標記化合物（２．４ｇ）を得た。
1H－NMR  Spectrum  (DMSO－d6)δ(ppm)：3.99(2H,s),  5.31(2H,s),　6.66(1H,s),  6.84－6.87(1H,m),  6.97－7.00(1H,m),  7.26(2H,d,J=8Hz),　7.39(2H,d,J=8Hz),  7.70－7.74(1H,m),  8.16－8.17(1H,m).
Figure JPOXMLDOC01-appb-C000049
 To a tetrahydrofuran solution (15 mL) of 2- (4- (5-tributylstannyl-isoxazol-3-ylmethyl) -benzyloxy) -pyridine (5.1 g) described in Preparation Example 1-8-1 at 0 ° C. Iodine (2.5 g) was added. The mixture was stirred for 20 minutes at that temperature. A 10% aqueous sodium thiosulfate solution and ethyl acetate were added to the mixture. The organic layer was separated, washed with saturated brine, dried over anhydrous magnesium sulfate, and filtered. The filtrate was concentrated, and the residue was purified by silica gel column chromatography (heptane: ethyl acetate = 10: 1 to 4: 1) to obtain the title compound (2.4 g).
1 H-NMR Spectrum (DMSO-d 6 ) δ (ppm): 3.99 (2H, s), 5.31 (2H, s), 6.66 (1H, s), 6.84-6.87 (1H, m), 6.97-7.00 ( 1H, m), 7.26 (2H, d, J = 8Hz), 7.39 (2H, d, J = 8Hz), 7.70-7.74 (1H, m), 8.16-8.17 (1H, m).
　以下、参考例１に記載の３－（３－（４－（ピリジン－２－イルオキシメチル）－ベンジル）－イソキサゾール－５－イル）－ピリジン－２－イルアミンの製造例の別法を説明する。
［参考例２］ｔｅｒｔ－ブチル　（３－アセチルピリジン－２－イル）カルバメートの合成
Figure JPOXMLDOC01-appb-C000050
 　窒素雰囲気下、１－（２－アミノピリジン－３－イル）エタノン（５０ｇ，３６８ｍｍｏｌ）、ジ－ｔｅｒｔ－ブチルジカーボネイト（１２０ｇ，５５２ｍｍｏｌ）とｔｅｒｔ－ブタノール（２００ｍＬ）の混合物を９０℃で３時間撹拌した。冷却後、減圧下溶媒を留去し、残渣にｎ－ヘプタン（５００ｍＬ）を加え、析出している固体を濾取し、標記化合物（７７ｇ）を黄色固体として得た。
1H-NMR  Spectrum  (CDCl3)δ(ppm):1.54(9H,s),  2.64(3H,s),　7.03(1H,dd,J＝4.8,8.0Hz),  8.16(1H,dd,J＝2.0,8.0Hz),  8.63(1H,dd,J＝2.0,4.8Hz),  10.82(1H,brs). Hereinafter, another method for producing 3- (3- (4- (pyridin-2-yloxymethyl) -benzyl) -isoxazol-5-yl) -pyridin-2-ylamine described in Reference Example 1 will be described. .
[Reference Example 2] Synthesis of tert-butyl (3-acetylpyridin-2-yl) carbamate
Figure JPOXMLDOC01-appb-C000050
 In a nitrogen atmosphere, a mixture of 1- (2-aminopyridin-3-yl) ethanone (50 g, 368 mmol), di-tert-butyl dicarbonate (120 g, 552 mmol) and tert-butanol (200 mL) was stirred at 90 ° C. for 3 hours. Stir. After cooling, the solvent was evaporated under reduced pressure, n-heptane (500 mL) was added to the residue, and the precipitated solid was collected by filtration to obtain the title compound (77 g) as a yellow solid.
1 H-NMR Spectrum (CDCl 3 ) δ (ppm): 1.54 (9H, s), 2.64 (3H, s), 7.03 (1H, dd, J = 4.8, 8.0 Hz), 8.16 (1H, dd, J = 2.0, 8.0Hz), 8.63 (1H, dd, J = 2.0, 4.8Hz), 10.82 (1H, brs).
［参考例３］エチル　５－（２－アミノピリジン－３－イル）イソキサゾール－３－カルボキシレートの合成
Figure JPOXMLDOC01-appb-C000051
 　窒素雰囲気下、ｔｅｒｔ－ブチル　（３－アセチルピリジン－２－イル）カルバメート（６００ｍｇ，２．２９ｍｍｏｌ）、シュウ酸ジエチル（６６９ｍｇ，４．５８ｍｍｏｌ）のトルエン（５．０ｍＬ）溶液に、室温でカリウムｔｅｒｔ－ブトキシド（５１４ｍｇ，４．５８ｍｍｏｌ）を加え、２時間撹拌した。トルエン（５．０ｍＬ）を加えて１時間撹拌した後、カリウムｔｅｒｔ－ブトキシド（２５７ｍｇ，２．２９ｍｍｏｌ）を加え、２時間撹拌した。反応混合液へ塩酸ヒドロキシルアミン（４７７ｍｇ，６．８７ｍｍｏｌ）とエタノール（１０ｍＬ）を加えて１時間撹拌した後、水（１．０ｍＬ）を加え、室温で終夜撹拌した。水（３０ｍＬ）を加え、酢酸エチルで抽出した。有機層を飽和食塩水で洗浄し、無水硫酸マグネシウムで乾燥した後、濃縮した。濃縮残渣をＮ，Ｎ－ジメチルホルムアミド（５ｍＬ）に溶解し、トリエチルアミン（１９２ｍｇ）を加え、８０℃で６時間撹拌した。冷却後、水を加え、酢酸エチルで抽出した。有機層を飽和食塩水で洗浄し、無水硫酸マグネシウムで乾燥した後、減圧下溶媒を留去し、標記化合物（４４３ｍｇ）を白色固体として得た。
1H-NMR  Spectrum  (CDCl3)δ(ppm):1.45(3H,t,J＝7.2Hz),  4.49(2H,q,J＝7.2Hz),  5.40(2H,brs),　6.79(1H,dd,J＝5.2,7.6Hz),  6.91(1H,s),　7.81(1H,dd,J＝2.0,7.6Hz),  8.21(1H,dd,J＝2.0,5.2Hz). [Reference Example 3] Synthesis of ethyl 5- (2-aminopyridin-3-yl) isoxazole-3-carboxylate
Figure JPOXMLDOC01-appb-C000051
 To a solution of tert-butyl (3-acetylpyridin-2-yl) carbamate (600 mg, 2.29 mmol) and diethyl oxalate (669 mg, 4.58 mmol) in toluene (5.0 mL) under a nitrogen atmosphere at room temperature -Butoxide (514 mg, 4.58 mmol) was added and stirred for 2 hours. After adding toluene (5.0 mL) and stirring for 1 hour, potassium tert-butoxide (257 mg, 2.29 mmol) was added and stirred for 2 hours. Hydroxylamine hydrochloride (477 mg, 6.87 mmol) and ethanol (10 mL) were added to the reaction mixture, and the mixture was stirred for 1 hr, water (1.0 mL) was added, and the mixture was stirred at room temperature overnight. Water (30 mL) was added and extracted with ethyl acetate. The organic layer was washed with saturated brine, dried over anhydrous magnesium sulfate, and concentrated. The concentrated residue was dissolved in N, N-dimethylformamide (5 mL), triethylamine (192 mg) was added, and the mixture was stirred at 80 ° C. for 6 hr. After cooling, water was added and extracted with ethyl acetate. The organic layer was washed with saturated brine, dried over anhydrous magnesium sulfate, and the solvent was evaporated under reduced pressure to give the title compound (443 mg) as a white solid.
1 H-NMR Spectrum (CDCl 3 ) δ (ppm): 1.45 (3H, t, J = 7.2Hz), 4.49 (2H, q, J = 7.2Hz), 5.40 (2H, brs), 6.79 (1H, dd , J = 5.2, 7.6Hz), 6.91 (1H, s), 7.81 (1H, dd, J = 2.0, 7.6Hz), 8.21 (1H, dd, J = 2.0, 5.2Hz).
［参考例４］[５－（２－アミノピリジン－３－イル）イソキサゾール－３－イル]メタノールの合成
Figure JPOXMLDOC01-appb-C000052
 　窒素雰囲気下、エチル　５－（２－アミノピリジン－３－イル）イソキサゾール－３－カルボキシレート（３８１ｍｇ，１．６３ｍｍｏｌ）のテトラヒドロフラン（３．８ｍＬ）とエタノール（３．８ｍＬ）の懸濁液に、０℃で水素化ホウ素ナトリウム（２０１ｍｇ，４．８９ｍｍｏｌ）を加え、０℃で１時間、２０℃で２１時間撹拌した。氷水浴冷却下、反応混合液へ２Ｎ塩酸（２．４６ｍＬ，４．８９ｍｍｏｌ）を滴下し、０℃で１０分間、室温で３０分間撹拌した。氷水浴冷却下、５％炭酸水素ナトリウム水溶液を滴下して塩基性とした後、酢酸エチルで抽出した。有機層を無水硫酸マグネシウムで乾燥した後、減圧下溶媒を留去した。残渣をテトラヒドロフラン（１．４ｍＬ）に懸濁し、０℃で水素化ホウ素ナトリウム（６７ｍｇ，１．６３ｍｍｏｌ）を加え、メタノール（１．４ｍＬ）で洗い込んだ。室温で１時間撹拌した後、６０℃で５時間撹拌した。氷水浴冷却下、反応混合液へ１Ｎ塩酸（１．６３ｍＬ，１．６３ｍｍｏｌ）を滴下し、０℃で１０分間、室温で３０分間撹拌した。氷水浴冷却下、１Ｎ水酸化ナトリウム水溶液を滴下して塩基性とした後、酢酸エチルで抽出した。有機層を無水硫酸マグネシウムで乾燥した後、減圧下溶媒を留去し、標記化合物（２５８ｍｇ）を淡黄色固体として得た。
1H-NMR  Spectrum  (DMSO－d6)δ(ppm):4.56(2H,d,J＝5.6Hz),  5.54(1H,t,J＝5.6Hz),  6.27(2H,brs),　6.72(1H,dd,J＝4.8,7.6Hz),  6.90(1H,s),　7.90(1H,dd,J＝2.0,7.6Hz),  8.10(1H,dd,J＝2.0,4.8Hz). Reference Example 4 Synthesis of [5- (2-aminopyridin-3-yl) isoxazol-3-yl] methanol
Figure JPOXMLDOC01-appb-C000052
 Under a nitrogen atmosphere, a suspension of ethyl 5- (2-aminopyridin-3-yl) isoxazole-3-carboxylate (381 mg, 1.63 mmol) in tetrahydrofuran (3.8 mL) and ethanol (3.8 mL) was added. Sodium borohydride (201 mg, 4.89 mmol) was added at 0 ° C., and the mixture was stirred at 0 ° C. for 1 hour and at 20 ° C. for 21 hours. 2N hydrochloric acid (2.46 mL, 4.89 mmol) was added dropwise to the reaction mixture while cooling in an ice-water bath, and the mixture was stirred at 0 ° C. for 10 minutes and at room temperature for 30 minutes. While cooling with an ice-water bath, a 5% aqueous sodium hydrogen carbonate solution was added dropwise to make the solution basic, followed by extraction with ethyl acetate. After drying the organic layer with anhydrous magnesium sulfate, the solvent was distilled off under reduced pressure. The residue was suspended in tetrahydrofuran (1.4 mL), sodium borohydride (67 mg, 1.63 mmol) was added at 0 ° C., and the mixture was washed with methanol (1.4 mL). After stirring at room temperature for 1 hour, it stirred at 60 degreeC for 5 hours. 1N hydrochloric acid (1.63 mL, 1.63 mmol) was added dropwise to the reaction mixture while cooling in an ice-water bath, and the mixture was stirred at 0 ° C. for 10 minutes and at room temperature for 30 minutes. Under cooling with an ice-water bath, 1N aqueous sodium hydroxide solution was added dropwise to make the solution basic, followed by extraction with ethyl acetate. The organic layer was dried over anhydrous magnesium sulfate, and then the solvent was distilled off under reduced pressure to obtain the title compound (258 mg) as a pale yellow solid.
1 H-NMR Spectrum (DMSO-d 6 ) δ (ppm): 4.56 (2H, d, J = 5.6Hz), 5.54 (1H, t, J = 5.6Hz), 6.27 (2H, brs), 6.72 (1H , dd, J = 4.8,7.6Hz), 6.90 (1H, s), 7.90 (1H, dd, J = 2.0,7.6Hz), 8.10 (1H, dd, J = 2.0,4.8Hz).
　参考例５～１０は、参考例３および４の別途合成法である。 Reference Examples 5 to 10 are separate synthesis methods of Reference Examples 3 and 4.
［参考例５］　Ｎ－（３－アセチルピリジン－２－イル）－２，２－ジメチルプロパナミドの合成
Figure JPOXMLDOC01-appb-C000053
 　１－（２－アミノピリジン－３－イル）エタノン（２７２ｍｇ，２ｍｍｏｌ）、４－ジメチルアミノピリジン（２４ｍｇ，０．２ｍｍｏｌ）、トリエチルアミン（０．６４ｍＬ，４．６ｍｍｏｌ）、およびトルエン（２ｍＬ）の混合物に室温で塩化ピバロイル（０．５２ｍＬ，４．２ｍｍｏｌ）を滴下し、室温で１時間、６０℃で５時間攪拌した。２－ｔｅｒｔ－ブチル－４－メチル－４Ｈ－ピリド［２，３－ｄ］［１，３］オキサジン－４－イル　ピバレート*の生成を確認後、反応混合物に水（２ｍＬ）および５Ｎ塩酸（０．８ｍＬ）を加え、室温で３０分間攪拌した。反応混合物を分液し、水層に５Ｎ水酸化ナトリウム水溶液（１ｍＬ）を加えて、トルエンで抽出した。溶媒を減圧下留去し、途中析出した固形分を濾過し、標記化合物（４１５ｍｇ）を得た。
1H-NMR  Spectrum  (CDCl3)δ(ppm):1.33(9H,s),  2.64(3H,s),　7.10(1H,dd,J＝4.8,8.0Hz),  8.17(1H,dd,J＝2.0,7.6Hz),  8.64(1H,dd,J=2.0,4.8Hz).

*２－ｔｅｒｔ－ブチル－４－メチル－４Ｈ－ピリド［２，３－ｄ］［１，３］オキサジン－４－イル　ピバレート
1H-NMR  Spectrum  (CDCl3)δ(ppm):1.09(9H,s),  1.32(9H,s),　2.05(3H,s),  7.14(1H,dd,J＝4.8,7.6Hz),  7.71(1H,dd,J＝2.0,7.6Hz),  8.51(1H,dd,J＝2.0,4.8Hz). [Reference Example 5] Synthesis of N- (3-acetylpyridin-2-yl) -2,2-dimethylpropanamide
Figure JPOXMLDOC01-appb-C000053
 A mixture of 1- (2-aminopyridin-3-yl) ethanone (272 mg, 2 mmol), 4-dimethylaminopyridine (24 mg, 0.2 mmol), triethylamine (0.64 mL, 4.6 mmol), and toluene (2 mL) Was added dropwise at room temperature with pivaloyl chloride (0.52 mL, 4.2 mmol), and stirred at room temperature for 1 hour and at 60 ° C. for 5 hours. After confirming the formation of 2-tert-butyl-4-methyl-4H-pyrido [2,3-d] [1,3] oxazin-4-yl pivalate * , the reaction mixture was mixed with water (2 mL) and 5N hydrochloric acid (0 8 mL) and stirred at room temperature for 30 minutes. The reaction mixture was separated, 5N aqueous sodium hydroxide solution (1 mL) was added to the aqueous layer, and the mixture was extracted with toluene. The solvent was distilled off under reduced pressure, and the solid content precipitated during the filtration was filtered to obtain the title compound (415 mg).
1 H-NMR Spectrum (CDCl 3 ) δ (ppm): 1.33 (9H, s), 2.64 (3H, s), 7.10 (1H, dd, J = 4.8, 8.0 Hz), 8.17 (1H, dd, J = 2.0,7.6Hz), 8.64 (1H, dd, J = 2.0,4.8Hz).

* 2-tert-butyl-4-methyl-4H-pyrido [2,3-d] [1,3] oxazin-4-yl pivalate
1 H-NMR Spectrum (CDCl 3 ) δ (ppm): 1.09 (9H, s), 1.32 (9H, s), 2.05 (3H, s), 7.14 (1H, dd, J = 4.8, 7.6 Hz), 7.71 (1H, dd, J = 2.0,7.6Hz), 8.51 (1H, dd, J = 2.0,4.8Hz).
　参考例６～７は、参考例５の別途合成法である。 Reference Examples 6 to 7 are separate synthesis methods of Reference Example 5.
［参考例６］２－ｔｅｒｔ－ブチル－４Ｈ－ピリド［２，３－ｄ］［１，３］オキサジン－４－オンの合成
Figure JPOXMLDOC01-appb-C000054
 　２－アミノニコチン酸（１３．８ｇ，１００ｍｍｏｌ）、４－ジメチルアミノピリジン（１．２ｇ，１０ｍｍｏｌ）、トリエチルアミン（５５．８ｍＬ，４００ｍｍｏｌ）、およびＮ－メチルピロリドン（１４０ｍＬ，４２ｍｍｏｌ）の混合物に０℃で塩化ピバロイル（２４．１ｇ，２００ｍｍｏｌ）を滴下し、滴下終了後室温で終夜攪拌した。反応混合物に水を加え、トルエンで抽出し、有機層を水と飽和食塩水で洗浄した。無水硫酸マグネシウムで乾燥し、濾過後、溶媒を減圧下留去した。残渣にｎ－ヘプタンを加え、０℃で懸濁撹拌した後、濾過し、標記化合物（１６．６ｇ）を得た。
1H-NMR  Spectrum  (CDCl3)δ(ppm):1.45(9H,s),  7.48(1H,dd,J＝4.8,8.0Hz),  8.52(1H,dd,J＝2.0,7.6Hz),  8.97(1H,dd,J＝2.0,4.8Hz). [Reference Example 6] Synthesis of 2-tert-butyl-4H-pyrido [2,3-d] [1,3] oxazin-4-one
Figure JPOXMLDOC01-appb-C000054
 To a mixture of 2-aminonicotinic acid (13.8 g, 100 mmol), 4-dimethylaminopyridine (1.2 g, 10 mmol), triethylamine (55.8 mL, 400 mmol), and N-methylpyrrolidone (140 mL, 42 mmol) at 0 ° C. Then, pivaloyl chloride (24.1 g, 200 mmol) was added dropwise, and after completion of the dropwise addition, the mixture was stirred overnight at room temperature. Water was added to the reaction mixture, extraction was performed with toluene, and the organic layer was washed with water and saturated brine. After drying over anhydrous magnesium sulfate and filtration, the solvent was distilled off under reduced pressure. N-Heptane was added to the residue, and the mixture was suspended and stirred at 0 ° C., followed by filtration to obtain the title compound (16.6 g).
1 H-NMR Spectrum (CDCl 3 ) δ (ppm): 1.45 (9H, s), 7.48 (1H, dd, J = 4.8, 8.0 Hz), 8.52 (1H, dd, J = 2.0, 7.6 Hz), 8.97 (1H, dd, J = 2.0,4.8Hz).
［参考例７］Ｎ－（３－アセチルピリジン－２－イル）－２，２－ジメチルプロパナミドの合成
Figure JPOXMLDOC01-appb-C000055
 　２－ｔｅｒｔ－ブチル－４Ｈ－ピリド［２，３－ｄ］［１，３］オキサジン－４－オン（１０．２ｇ，５０ｍｍｏｌ）およびテトラヒドロフラン（５０ｍＬ）の混合物に－７８℃でメチルマグネシウムブロミド（０．９７Ｍ　テトラヒドロフラン溶液，１００ｍＬ，９７ｍｍｏｌ）を滴下し、滴下終了後－７８℃で３０分間攪拌した。反応混合物に飽和塩化アンモニウム水溶液と水を加え、酢酸エチルで抽出し、有機層を塩化アンモニウム水溶液で洗浄した。溶媒を減圧下留去し、途中析出した固形分を濾過し、標記化合物（９．１ｇ）を得た。
1H-NMR  Spectrum  (CDCl3)δ(ppm):1.33(9H,s),  2.64(3H,s),　7.10(1H,dd,J＝4.8,8.0Hz),  8.17(1H,dd,J＝2.0,7.6Hz),  8.64(1H,dd,J＝2.0,4.8Hz). [Reference Example 7] Synthesis of N- (3-acetylpyridin-2-yl) -2,2-dimethylpropanamide
Figure JPOXMLDOC01-appb-C000055
 To a mixture of 2-tert-butyl-4H-pyrido [2,3-d] [1,3] oxazin-4-one (10.2 g, 50 mmol) and tetrahydrofuran (50 mL) at −78 ° C. methyl magnesium bromide (0 97M tetrahydrofuran solution, 100 mL, 97 mmol) was added dropwise, and the mixture was stirred at −78 ° C. for 30 minutes after completion of the dropwise addition. A saturated aqueous ammonium chloride solution and water were added to the reaction mixture, followed by extraction with ethyl acetate, and the organic layer was washed with an aqueous ammonium chloride solution. The solvent was distilled off under reduced pressure, and the solid content precipitated during the filtration was filtered to obtain the title compound (9.1 g).
1 H-NMR Spectrum (CDCl 3 ) δ (ppm): 1.33 (9H, s), 2.64 (3H, s), 7.10 (1H, dd, J = 4.8, 8.0 Hz), 8.17 (1H, dd, J = 2.0, 7.6Hz), 8.64 (1H, dd, J = 2.0, 4.8Hz).
［参考例８］エチル　５－｛２－［（２，２－ジメチルプロパノイル）アミノ］ピリジン－３－イル｝イソキサゾール－３－カルボキシレートの合成
Figure JPOXMLDOC01-appb-C000056
 　Ｎ－（３－アセチルピリジン－２－イル）－２，２－ジメチルプロパナミド（８．０８ｇ，３６．７ｍｍｏｌ）、シュウ酸ジエチル（１０．０ｍＬ，７３．４ｍｍｏｌ）、およびエタノール（３６ｍＬ）の混合物に－２５℃でカリウムｔｅｒｔ－ブトキシド（８．２３ｇ，７３．４ｍｍｏｌ）を加え、－２５℃で１時間攪拌した。反応混合物に水（７２ｍＬ）を加えて室温で攪拌後、トルエン（３６ｍＬ）を加えて分層し、得られた水層をさらにトルエン（３６ｍＬ）で洗浄した。５Ｎ塩酸（１４ｍＬ）および塩酸ヒドロキシルアミン（５．１０ｇ，７３．４ｍｍｏｌ）を加え、室温で３０分間攪拌した。反応混合物に５Ｎ水酸化ナトリウム水溶液（１４ｍＬ）を加え、トルエンで抽出した後、溶媒を減圧下留去した。得られた残渣にエタノール（３５ｍＬ）およびトリエチルアミン（５ｍＬ）を加え、８０℃から８５℃で６時間攪拌した。反応混合物にｎ－ヘプタン（１０５ｍＬ）を加え、析出した固体を濾過し、標記化合物（６．９０ｇ）を得た。
1H-NMR  Spectrum  (DMSO－d6)δ(ppm):1.19(9H,s),  1.32(3H,t),　4.37(4H,q),  7.12(1H,s),　7.46(1H,dd,J＝4.8,8.0Hz),  8.25(1H,dd,J＝2.0,8.0Hz),  8.58(1H,dd,J＝2.0,4.8Hz),  10.03(1H,s). [Reference Example 8] Synthesis of ethyl 5- {2-[(2,2-dimethylpropanoyl) amino] pyridin-3-yl} isoxazole-3-carboxylate
Figure JPOXMLDOC01-appb-C000056
 Of N- (3-acetylpyridin-2-yl) -2,2-dimethylpropanamide (8.08 g, 36.7 mmol), diethyl oxalate (10.0 mL, 73.4 mmol), and ethanol (36 mL) To the mixture was added potassium tert-butoxide (8.23 g, 73.4 mmol) at −25 ° C., and the mixture was stirred at −25 ° C. for 1 hour. Water (72 mL) was added to the reaction mixture, and the mixture was stirred at room temperature. Toluene (36 mL) was added and the layers were separated, and the resulting aqueous layer was further washed with toluene (36 mL). 5N hydrochloric acid (14 mL) and hydroxylamine hydrochloride (5.10 g, 73.4 mmol) were added, and the mixture was stirred at room temperature for 30 min. 5N Aqueous sodium hydroxide solution (14 mL) was added to the reaction mixture, and the mixture was extracted with toluene. The solvent was evaporated under reduced pressure. Ethanol (35 mL) and triethylamine (5 mL) were added to the resulting residue, and the mixture was stirred at 80 to 85 ° C. for 6 hours. N-Heptane (105 mL) was added to the reaction mixture, and the precipitated solid was filtered to obtain the title compound (6.90 g).
1 H-NMR Spectrum (DMSO-d6) δ (ppm): 1.19 (9H, s), 1.32 (3H, t), 4.37 (4H, q), 7.12 (1H, s), 7.46 (1H, dd, J = 4.8, 8.0 Hz), 8.25 (1 H, dd, J = 2.0, 8.0 Hz), 8.58 (1 H, dd, J = 2.0, 4.8 Hz), 10.03 (1 H, s).
［参考例９］Ｎ－｛３－［３－（ヒドロキシメチル）イソキサゾール－５－イル］ピリジン－２－イル｝－２，２ジメチルプロパナミドの合成
Figure JPOXMLDOC01-appb-C000057
 　エチル　５－｛２－［（２，２－ジメチルプロパノイル）アミノ］ピリジン－３－イル｝イソキサゾール－３－カルボキシレート（１１１ｇ，３５０ｍｍｏｌ）、エタノール（１１０ｍＬ）、およびテトラヒドロフラン（３５０ｍＬ）の混合物に室温で水素化ホウ素ナトリウム（１３．２ｇ，３５０ｍｍｏｌ）を加え、室温で６時間攪拌した。反応混合物に水（３５０ｍＬ）および５Ｎ塩酸（９０ｍＬ）を加え、室温で３０分間攪拌後、５Ｎ水酸化ナトリウム水溶液（１１０ｍＬ）を加え、酢酸エチルとテトラヒドロフランの混合液で抽出し、有機層を水と飽和食塩水で洗浄した。溶媒を減圧下留去し、［５－（２－アミノピリジン－３－イル）イソキサゾール－３－イル］メタノールが一部混入した標記化合物（８３．８ｇ）を黄色固体として得た。
1H-NMR  Spectrum  (DMSO－d6)δ(ppm):1.20(9H,s),  4.52(2H,d,J＝6.0Hz),  5.53(1H,t,J＝6.0Hz),  6.70(1H,s),　7.44(1H,dd,J＝4.8,8.0Hz),  8.19(1H,dd,J＝5.6,7.6Hz),  8.53(1H,dd,J＝2.0,4.8Hz),  9.89(1H,brs). [Reference Example 9] Synthesis of N- {3- [3- (hydroxymethyl) isoxazol-5-yl] pyridin-2-yl} -2,2 dimethylpropanamide
Figure JPOXMLDOC01-appb-C000057
 To a mixture of ethyl 5- {2-[(2,2-dimethylpropanoyl) amino] pyridin-3-yl} isoxazole-3-carboxylate (111 g, 350 mmol), ethanol (110 mL), and tetrahydrofuran (350 mL) at room temperature Added sodium borohydride (13.2 g, 350 mmol) and stirred at room temperature for 6 hours. Water (350 mL) and 5N hydrochloric acid (90 mL) were added to the reaction mixture, and the mixture was stirred at room temperature for 30 minutes, 5N aqueous sodium hydroxide solution (110 mL) was added, and the mixture was extracted with a mixture of ethyl acetate and tetrahydrofuran. Washed with saturated brine. The solvent was distilled off under reduced pressure to obtain the title compound (83.8 g) partially contaminated with [5- (2-aminopyridin-3-yl) isoxazol-3-yl] methanol as a yellow solid.
1 H-NMR Spectrum (DMSO-d 6 ) δ (ppm): 1.20 (9H, s), 4.52 (2H, d, J = 6.0Hz), 5.53 (1H, t, J = 6.0Hz), 6.70 (1H , s), 7.44 (1H, dd, J = 4.8,8.0Hz), 8.19 (1H, dd, J = 5.6,7.6Hz), 8.53 (1H, dd, J = 2.0,4.8Hz), 9.89 (1H, brs).
［参考例１０］[５－（２－アミノピリジン－３－イル）イソキサゾール－３－イル]メタノールの合成
Figure JPOXMLDOC01-appb-C000058
 　参考例９にて得たＮ－｛３－［３－（ヒドロキシメチル）イソキサゾール－５－イル］ピリジン－２－イル｝－２，２ジメチルプロパナミド（８２．８ｇ）およびメタノール（３５０ｍＬ）の混合物に室温で５Ｎ水酸化ナトリウム水溶液（３５０ｍＬ）を加え、５７～６０℃で１４時間攪拌した。反応混合物に酢酸（１００ｍＬ）を加え、析出した固体を濾過し、標記化合物（４２．２ｇ）を灰白色固体として得た。
1H-NMR  Spectrum  (DMSO－d6)δ(ppm):4.54(2H,s),  5.57(1H,brs),　6.25(2H,brs),  6.71(1H,dd,J＝4.8,8.0Hz),  6.90(1H,s),　7.90(1H,dd,J＝1.6,7.6Hz),  8.09(1H,dd,J＝1.6,4.8Hz). [Reference Example 10] Synthesis of [5- (2-aminopyridin-3-yl) isoxazol-3-yl] methanol
Figure JPOXMLDOC01-appb-C000058
 N- {3- [3- (hydroxymethyl) isoxazol-5-yl] pyridin-2-yl} -2,2 dimethylpropanamide (82.8 g) and methanol (350 mL) obtained in Reference Example 9 To the mixture was added 5N aqueous sodium hydroxide solution (350 mL) at room temperature, and the mixture was stirred at 57-60 ° C. for 14 hours. Acetic acid (100 mL) was added to the reaction mixture, and the precipitated solid was filtered to obtain the title compound (42.2 g) as an off-white solid.
1 H-NMR Spectrum (DMSO-d 6 ) δ (ppm): 4.54 (2H, s), 5.57 (1H, brs), 6.25 (2H, brs), 6.71 (1H, dd, J = 4.8, 8.0 Hz) , 6.90 (1H, s), 7.90 (1H, dd, J = 1.6, 7.6Hz), 8.09 (1H, dd, J = 1.6, 4.8Hz).
　参考例１１～１３は、参考例５～１０の別途合成法である。 Reference Examples 11 to 13 are separate synthesis methods of Reference Examples 5 to 10.
［参考例１１］　Ｎ－（３－アセチルピリジン－２－イル）－２，２－ジメチルプロパナミドの合成
Figure JPOXMLDOC01-appb-C000059
 　１５００Ｌ反応缶に１－（２－アミノピリジン－３－イル）エタノン（４０．０ｋｇ，２９４ｍｏｌ）を加えた後、トルエン（約１５ｋｇ）で洗い込んだ。続いて、トルエンが合計３４７ｋｇになるようにトルエンを加えた後、塩化ピバロイル（５３．１ｋｇ，１．５Ｍ／Ｍ）を加えた。トリエチルアミン（２３．８ｋｇ，０．８Ｍ／Ｍ）を内温３０℃以下で滴下し、内温２０～３０℃で１時間以上撹拌した。再びトリエチルアミン（２３．８ｋｇ，０．８Ｍ／Ｍ）を内温３０℃以下で滴下後、内温２０～３０℃で２時間以上攪拌し、反応が終了していることをＨＰＬＣにて確認した。
　ブライン冷却下、水（１００Ｌ）を内温３０℃以下で滴下し、続いて、３５％塩酸（４９．０ｋｇ，１．６Ｍ／Ｍ）を内温３０℃以下で滴下した。反応溶液を５分間攪拌後、１５分間以上静置し、下層（ａ）をポリ容器に取り分けた。水（１００Ｌ）を加え、５分間攪拌した後、１５分以上静置した。下層（ｃ）をポリ容器に取り分け、上層（ｄ）を取り出した後、下層（ａ）および下層（ｃ）を１５００Ｌ反応缶へ戻した。ブライン冷却下、酢酸エチル（２８９ｋｇ）を加え、続いて４８．７％水酸化ナトリウム水溶液（４３．４ｋｇ，１．８Ｍ／Ｍ）を内温３０℃以下で滴下し、５分間攪拌後、下層のｐＨが８～９であることをＵＮＩＶ試験紙にて確認した。１５分以上静置した後、下層（ｅ）、上層（ｆ）をそれぞれ取り分け、下層（ｅ）を１５００Ｌ反応缶へ戻した。酢酸エチル（１４４ｋｇ）を加え、５分間攪拌後、１５分間以上静置し、下層（ｇ）、上層（ｈ）をそれぞれ取り分けた。下層（ｇ）を１５００Ｌ反応缶へ戻して酢酸エチル（１４４ｋｇ）を加え、５分間攪拌後、１５分以上静置した。下層（ｉ）を取り出した後、上層（ｆ）と上層（ｈ）を１５００Ｌ反応缶へ戻し、酢酸エチル（約１５ｋｇ）で洗い込んだ。
　１５００Ｌ反応缶に戻した有機層を減圧濃縮し（温水５０℃）、濃縮液が約２００Ｌになった時点で濃縮を一旦終了した。濃縮液をＳＵＳ容器に取り出し、缶内をトルエン（１７ｋｇ）で洗い出した。取り出した濃縮液の約半量を３００Ｌ反応缶へ入れ，トルエン（９ｋｇ）で洗い込んだ。濃縮液をさらに減圧濃縮し（温水５０℃）、コンデンサーからの留出量が減ったところで残りの濃縮液を３００Ｌ反応缶へ入れ、トルエン（９ｋｇ）で洗い込んだ。減圧濃縮を再開した（温水５０℃～７０℃）。留出が殆んど無くなった時点で、水冷却を開始し、内温５０℃以下でトルエン（５２ｋｇ）を加えた。減圧濃縮を再開した（温水５０～８０℃）。外温８０℃、減圧度－０．０９０ＭＰａ以上で留出を認めなくなった時点で濃縮を終了し、内温２０～３０℃でエタノール（６１ｋｇ）を加えた。
　窒素雰囲気下，缶内のエタノール溶液をＳＵＳ容器に取り出し、エタノール（１３ｋｇ）で洗い出した。取り出した溶液を１５００Ｌ反応缶へ加えた後、エタノール（１３ｋｇ）で洗い込み、標記化合物のエタノール溶液（目的物を６９．４ｋｇ含有，収率：１０７．３％）を得た。
ＨＰＬＣ条件　カラム：YMC-Pack　Pro  C18　(5μm,　150x4.6mmI.D.,　YMC)，移動相：アセトニトリル／水／酢酸アンモニウム=３００／７００／１～９００／１００／１（ｖ／ｖ／ｗ）。 [Reference Example 11] Synthesis of N- (3-acetylpyridin-2-yl) -2,2-dimethylpropanamide
Figure JPOXMLDOC01-appb-C000059
 1- (2-Aminopyridin-3-yl) ethanone (40.0 kg, 294 mol) was added to a 1500 L reactor, and then washed with toluene (about 15 kg). Subsequently, toluene was added so that the total amount of toluene was 347 kg, and then pivaloyl chloride (53.1 kg, 1.5 M / M) was added. Triethylamine (23.8 kg, 0.8 M / M) was added dropwise at an internal temperature of 30 ° C. or lower, and the mixture was stirred at an internal temperature of 20-30 ° C. for 1 hour or longer. Triethylamine (23.8 kg, 0.8 M / M) was added dropwise again at an internal temperature of 30 ° C. or lower, and the mixture was stirred at an internal temperature of 20-30 ° C. for 2 hours or longer. It was confirmed by HPLC that the reaction was complete.
Under brine cooling, water (100 L) was added dropwise at an internal temperature of 30 ° C. or lower, and then 35% hydrochloric acid (49.0 kg, 1.6 M / M) was added dropwise at an internal temperature of 30 ° C. or lower. The reaction solution was stirred for 5 minutes and then allowed to stand for 15 minutes or more, and the lower layer (a) was separated into a plastic container. Water (100 L) was added and stirred for 5 minutes, then allowed to stand for 15 minutes or longer. After the lower layer (c) was separated into a plastic container and the upper layer (d) was taken out, the lower layer (a) and the lower layer (c) were returned to the 1500 L reactor. While cooling with brine, ethyl acetate (289 kg) was added, followed by dropwise addition of 48.7% aqueous sodium hydroxide solution (43.4 kg, 1.8 M / M) at an internal temperature of 30 ° C. or lower and stirring for 5 minutes. It was confirmed with UNIV test paper that the pH was 8-9. After leaving still for 15 minutes or more, the lower layer (e) and the upper layer (f) were separated, respectively, and the lower layer (e) was returned to the 1500 L reactor. Ethyl acetate (144 kg) was added and stirred for 5 minutes, then allowed to stand for 15 minutes or longer, and the lower layer (g) and the upper layer (h) were separated. The lower layer (g) was returned to the 1500 L reactor, ethyl acetate (144 kg) was added, stirred for 5 minutes, and allowed to stand for 15 minutes or longer. After taking out the lower layer (i), the upper layer (f) and the upper layer (h) were returned to the 1500 L reactor and washed with ethyl acetate (about 15 kg).
The organic layer returned to the 1500 L reactor was concentrated under reduced pressure (warm water 50 ° C.), and the concentration was temporarily stopped when the concentrated liquid reached about 200 L. The concentrated solution was taken out into a SUS container, and the inside of the can was washed out with toluene (17 kg). About half of the concentrated liquid taken out was placed in a 300 L reactor and washed with toluene (9 kg). The concentrated solution was further concentrated under reduced pressure (hot water 50 ° C.), and when the amount of distillation from the condenser decreased, the remaining concentrated solution was put into a 300 L reaction vessel and washed with toluene (9 kg). Concentration under reduced pressure was resumed (hot water 50 ° C. to 70 ° C.). When almost no distillation occurred, water cooling was started, and toluene (52 kg) was added at an internal temperature of 50 ° C. or lower. Concentration under reduced pressure was resumed (hot water 50-80 ° C.). Concentration was terminated when no distillation was observed at an external temperature of 80 ° C. and a reduced pressure of −0.090 MPa or more, and ethanol (61 kg) was added at an internal temperature of 20-30 ° C.
Under a nitrogen atmosphere, the ethanol solution in the can was taken out into a SUS container and washed with ethanol (13 kg). The solution taken out was added to a 1500 L reactor, and then washed with ethanol (13 kg) to obtain an ethanol solution of the title compound (containing 69.4 kg of the desired product, yield: 107.3%).
HPLC conditions Column: YMC-Pack Pro C18 (5 μm, 150 × 4.6 mm I.D., YMC), mobile phase: acetonitrile / water / ammonium acetate = 300/700/1 to 900/100/1 (v / v / w) .
［参考例１２］エチル　５－｛２－［（２，２－ジメチルプロパノイル）アミノ］ピリジン－３－イル｝イソキサゾール－３－カルボキシレートの合成
Figure JPOXMLDOC01-appb-C000060
 　窒素気流下、１５００Ｌ反応缶中のＮ－（３－アセチルピリジン－２－イル）－２，２－ジメチルプロパナミドのエタノール溶液（前工程の収率を１００％と仮定、２９４ｍｏｌ）にシュウ酸ジエチル（６４．４ｋｇ，１．５Ｍ／Ｍ）を加えた。ブライン循環を開始し、予め冷却しておいた２２％ｔｅｒｔ－ブトキシカリウムエタノール溶液（２１２．５ｋｇ，１．４５Ｍ／Ｍ）を内温１０℃以下で滴下した。内温－５～１０℃で３０分以上攪拌後、反応が終了していることをＨＰＬＣにて確認した。
　次いで、ヒドロキシルアミン塩酸塩（４０．８ｋｇ，２．０Ｍ／Ｍ）を内温１０℃以下で加え、内温１０℃以下で１時間以上攪拌した。次に、予め調製し冷却しておいた含水エタノール（エタノール（１５．３ｋｇ）／水（５．２ｋｇ））を発熱に注意しながら内温２０℃以下で滴下し、水（５８２Ｌ）を内温３０℃以下で滴下した。温水（２８℃）循環に切り替え、内温２０～３０℃でエチル　４－｛２－［（２，２－ジメチルプロパノイル）アミノ］ピリジン－３－イル｝－２－（ヒドロキシイミノ）－４－オキソブタノエイト（約１０ｇ）を加えた。目視にて固体の析出を確認した後、内温１５～２５℃で終夜攪拌した。反応が終了していることをＨＰＬＣにて確認した後、溶液のｐＨが６．５０～７．００になるまで４８．７％水酸化ナトリウム水溶液を内温１０～２５℃で滴下した（１８．１ｋｇ使用）。内温１０～２０℃で３時間以上攪拌後、６回に分けて遠心分離機で固液分離を行った。各遠心毎に、予め調製した含水エタノール（エタノール（２．４ｋｇ）／水（１２ｋｇ））でケーキを洗浄した後、洗液の色が無色透明になるまで水（約２００Ｌ）で洗浄した。さらに３０分以上遠心分離を行った後、ｗｅｔ固体をポリ袋に取り出した。次いで、棚式乾燥機にて、４５～５０℃の温水循環下、減圧乾燥し、固体（７１．５２ｋｇ）を得た。
　次に、１５００Ｌ反応缶に上記で得られた固体（７１．４５ｋｇ）を加え、エタノール（約７ｋｇ）で洗い込んだ。続いて、エタノールを合計２２６ｋｇになるように加え、トリエチルアミン（２１．６ｋｇ，１Ｍ／Ｍ）を加えた。温水（７５℃）循環を開始し、内温７０～７５℃で１４～１６時間攪拌し、反応が終了していることをＨＰＬＣにて確認した。次いで、ｎ－ヘプタン（４８８．７ｋｇ）を内温５５～７５℃で滴下した。その後、内温５０～５３℃でエチル　５－｛２－［（２，２－ジメチルプロパノイル）アミノ］ピリジン－３－イル｝イソキサゾール－３－カルボキシレート（約５ｇ）を加え、内温４５～５０℃で固体が析出していることを目視にて確認した。次いで、温水の温度を徐々に下げ、内温１５℃以下まで冷却した後、さらに、ブラインもしくは冷水冷却により内温０～１０℃で終夜攪拌した。ろ過機を用いて懸濁液をろ過し、ｎ－ヘプタン／エタノール混合溶液（ｎ－ヘプタン（７０ｋｇ）／エタノール（１０ｋｇ））、次いでｎ－ヘプタン（８０ｋｇ）で洗浄した。窒素にて１５分以上乾燥を行った後、ｗｅｔ固体をＳＵＳ容器に取り出した。ｗｅｔ固体を棚式乾燥機にて、４５～５０℃の温水循環下、減圧乾燥し、標記化合物（５４．５５ｋｇ，収率：５８．６％）を得た。
1H-NMR  Spectrum  (DMSO－d6)δ(ppm):1.19(9H,s),  1.32(3H,t),　4.37(4H,q),  7.12(1H,s),　7.46(1H,dd,J＝4.8,8.0Hz),  8.25(1H,dd,J＝2.0,8.0Hz),  8.58(1H,dd,J＝2.0,4.8Hz),  10.03(1H,s).
ＨＰＬＣ条件　カラム：YMC-Pack　Pro  C18　(5μm,　150x4.6mmI.D.,　YMC)，移動相：アセトニトリル／水／酢酸アンモニウム＝３００／７００／１～９００／１００／１（ｖ／ｖ／ｗ）。 [Reference Example 12] Synthesis of ethyl 5- {2-[(2,2-dimethylpropanoyl) amino] pyridin-3-yl} isoxazole-3-carboxylate
Figure JPOXMLDOC01-appb-C000060
 Under nitrogen flow, oxalic acid was added to an ethanol solution of N- (3-acetylpyridin-2-yl) -2,2-dimethylpropanamide in a 1500 L reactor (assuming that the yield of the previous step was 100%, 294 mol). Diethyl (64.4 kg, 1.5 M / M) was added. Brine circulation was started, and a 22% tert-butoxy potassium ethanol solution (212.5 kg, 1.45 M / M) that had been cooled in advance was added dropwise at an internal temperature of 10 ° C. or lower. After stirring at an internal temperature of −5 to 10 ° C. for 30 minutes or longer, it was confirmed by HPLC that the reaction was complete.
Subsequently, hydroxylamine hydrochloride (40.8 kg, 2.0 M / M) was added at an internal temperature of 10 ° C. or lower, and the mixture was stirred at an internal temperature of 10 ° C. or lower for 1 hour or longer. Next, water ethanol (ethanol (15.3 kg) / water (5.2 kg)) prepared and cooled in advance was added dropwise at an internal temperature of 20 ° C. or less while paying attention to heat generation, and water (582 L) was added to the internal temperature. It was dripped at 30 ° C. or lower. Switching to warm water (28 ° C.) circulation, ethyl 4- {2-[(2,2-dimethylpropanoyl) amino] pyridin-3-yl} -2- (hydroxyimino) -4- at an internal temperature of 20-30 ° C. Oxobutanoate (about 10 g) was added. After confirming the precipitation of the solid by visual observation, the mixture was stirred overnight at an internal temperature of 15 to 25 ° C. After confirming the completion of the reaction by HPLC, a 48.7% aqueous sodium hydroxide solution was added dropwise at an internal temperature of 10 to 25 ° C. until the pH of the solution reached 6.50 to 7.00 (18. 1 kg used). After stirring for 3 hours or longer at an internal temperature of 10 to 20 ° C., solid-liquid separation was performed in 6 centrifuges. After each centrifugation, the cake was washed with water-containing ethanol (ethanol (2.4 kg) / water (12 kg)) prepared in advance, and then washed with water (about 200 L) until the color of the washing became colorless and transparent. After further centrifugation for 30 minutes or more, the wet solid was taken out into a plastic bag. Next, it was dried under reduced pressure in a shelf dryer at 45 to 50 ° C. with circulating hot water to obtain a solid (71.52 kg).
Next, the solid (71.45 kg) obtained above was added to a 1500 L reactor and washed with ethanol (about 7 kg). Subsequently, ethanol was added to a total of 226 kg, and triethylamine (21.6 kg, 1 M / M) was added. Warm water (75 ° C.) circulation was started, and the mixture was stirred at an internal temperature of 70 to 75 ° C. for 14 to 16 hours. It was confirmed by HPLC that the reaction was complete. Subsequently, n-heptane (488.7 kg) was added dropwise at an internal temperature of 55 to 75 ° C. Thereafter, ethyl 5- {2-[(2,2-dimethylpropanoyl) amino] pyridin-3-yl} isoxazole-3-carboxylate (about 5 g) was added at an internal temperature of 50 to 53 ° C., and an internal temperature of 45 to It was visually confirmed that a solid was precipitated at 50 ° C. Next, the temperature of the warm water was gradually lowered and cooled to an internal temperature of 15 ° C. or lower, and further stirred overnight at an internal temperature of 0 to 10 ° C. by cooling with brine or cold water. The suspension was filtered using a filter and washed with a mixed solution of n-heptane / ethanol (n-heptane (70 kg) / ethanol (10 kg)) and then n-heptane (80 kg). After drying with nitrogen for 15 minutes or more, the wet solid was taken out into a SUS container. The wet solid was dried under reduced pressure in a shelf dryer under hot water circulation at 45 to 50 ° C. to obtain the title compound (54.55 kg, yield: 58.6%).
1 H-NMR Spectrum (DMSO-d6) δ (ppm): 1.19 (9H, s), 1.32 (3H, t), 4.37 (4H, q), 7.12 (1H, s), 7.46 (1H, dd, J = 4.8, 8.0 Hz), 8.25 (1 H, dd, J = 2.0, 8.0 Hz), 8.58 (1 H, dd, J = 2.0, 4.8 Hz), 10.03 (1 H, s).
HPLC conditions Column: YMC-Pack Pro C18 (5 μm, 150 × 4.6 mm I.D., YMC), mobile phase: acetonitrile / water / ammonium acetate = 300/700/1 to 900/100/1 (v / v / w) .
［参考例１３］[５－（２－アミノピリジン－３－イル）イソキサゾール－３－イル]メタノールの合成
Figure JPOXMLDOC01-appb-C000061
 　窒素気流下，１５００Ｌ反応缶にエチル　５－｛２－［（２，２－ジメチルプロパノイル）アミノ］ピリジン－３－イル｝イソキサゾール－３－カルボキシレート（５４．５ｋｇ，１７２ｍｏｌ）を加え、メタノール（４．９ｋｇ）で洗い込んだ。続いて、メタノールが合計１０８ｋｇになるようにメタノールを加え、トリエチルアミン（８．７ｋｇ，０．５Ｍ／Ｍ）を連続して加えた。温水（６０℃）循環を開始後、内温５０～６０℃で２時間以上攪拌し、反応が終了していることをＨＰＬＣ（条件１）にて確認した。
　次いで、水冷却を開始し、内温３０℃以下でテトラヒドロフラン（１２１ｋｇ）を加えた。ブライン冷却に切り替え、窒素気流下、水素化ホウ素ナトリウム（７．１５ｋｇ，１．１Ｍ／Ｍ）を内温０～１０℃で５時間以上かけて分割添加した。水素化ホウ素ナトリウムの添加終了後、ジャケットを冷水（４．０℃）循環に切り替え、内温０～１０℃で終夜攪拌した。翌日、水素化ホウ素ナトリウム（１．３０ｋｇ，０．２Ｍ／Ｍ）を内温０～１０℃で１時間以上かけて分割添加した。ジャケットを冷水に切り替え、３時間以上かけて内温を２０～３０℃に昇温し、さらに、そのまま内温２０～３０℃で終夜攪拌を行った。翌日、反応の進行具合をＨＰＬＣにて確認したが、反応はほとんど進行していなかったため、再度冷却し、水素化ホウ素ナトリウム（１．３０ｋｇ，０．２Ｍ／Ｍ）を内温０～１０℃で分割添加した。内温０～１０℃で１時間以上攪拌した後、ジャケットを冷水循環に切り替え、２時間以上かけて内温１５～２５℃に昇温した。１時間以上攪拌した後、反応が終了していることをＨＰＬＣ（条件１）にて確認し、終夜攪拌した。
　翌日、４８．７％水酸化ナトリウム水溶液（７１ｋｇ，５Ｍ／Ｍ）を内温５０℃以下で滴下後、続いて水（１３３Ｌ）を内温５０℃以下で滴下した。温水（５０～８０℃）循環を開始し、内温５０～６０℃で２０時間以上攪拌した後、反応が終了していることをＨＰＬＣ（条件２）にて確認した。
　次いで、水冷却下、水（７３Ｌ）を滴下した。冷水（１５℃）冷却に切り替え、内温１５～３０℃で[５－（２－アミノピリジン－３－イル）イソキサゾール－３－イル]メタノールを加え、固体の析出を確認後、水（２１８Ｌ）を滴下し、続いてブライン冷却下、３５％塩酸（１１５ｋｇ）を内温１５～３０℃で滴下し、水（３Ｌ）で洗い込んだ。内温１５～３０℃で５分以上攪拌した後、ｐＨメーターにて反応溶液のｐＨが４．００～５．００であることを確認し、内温１５～３０℃で１時間以上攪拌した。次いで、溶液のｐＨが７．００～８．００になるまで４８．７％水酸化ナトリウム水溶液を滴下し（１７．１　ｋｇ使用）、終夜静置した。翌日、撹拌および減圧を開始し、コンデンサーからの留出を確認後、温水（４０℃）循環を開始した。温水（３５～４５℃）、減圧度６８ｃｍＨｇ以上、内温３０℃以上の条件下、１時間以上濃縮を行った。窒素にて減圧を解除し、水（約２０Ｌ）で缶壁に付着した固体を洗い込んだ。内温１５～３０℃で３時間以上撹拌し、終夜静置した。翌日、内温１５～２５℃の範囲内にあることを確認し、スラリー液を２回にわけて遠心分離機で固液分離した。各遠心毎に、水（約２００Ｌ）で洗浄し、液切れ後１時間遠心分離を行った後、ｗｅｔ固体をポリ袋に取り出した。次いで、棚式乾燥機にて、４５～５０℃の温水循環下、減圧乾燥し、標記化合物（２６．５７ｋｇ，収率：８０．９％）を得た。
1H－NMR  Spectrum  (DMSO－d6)δ(ppm):4.54(2H,s),  5.57(1H,brs),　6.25(2H,brs),  6.71(1H,dd,J＝4.8,8.0Hz),  6.90(1H,s),　7.90(1H,dd,J＝1.6,7.6Hz),  8.09(1H,dd,J＝1.6,4.8Hz).
ＨＰＬＣ条件１　カラム：YMC-Pack　Pro  C18　(5μm,　150x4.6mmI.D.,　YMC)，移動相：アセトニトリル／水／酢酸アンモニウム=３００／７００／１～９００／１００／１（ｖ／ｖ／ｗ）。
ＨＰＬＣ条件２　カラム：YMC-Pack　ODS-AQ　(5μm,　150x4.6mmI.D.,　YMC)，移動相：アセトニトリル／水／８５％リン酸／１－オクタンスルホン酸ナトリウム=１６１．３／８３８．７／１／１．１～９００／１００／１／１．１（ｖ／ｖ／ｖ／ｗ）。 Reference Example 13 Synthesis of [5- (2-aminopyridin-3-yl) isoxazol-3-yl] methanol
Figure JPOXMLDOC01-appb-C000061
 Under a nitrogen stream, ethyl 5- {2-[(2,2-dimethylpropanoyl) amino] pyridin-3-yl} isoxazole-3-carboxylate (54.5 kg, 172 mol) was added to a 1500 L reactor, and methanol ( 4.9 kg). Subsequently, methanol was added so that the total amount of methanol was 108 kg, and triethylamine (8.7 kg, 0.5 M / M) was continuously added. After starting the circulation of warm water (60 ° C.), the mixture was stirred at an internal temperature of 50 to 60 ° C. for 2 hours or longer, and it was confirmed by HPLC (Condition 1) that the reaction was completed.
Next, water cooling was started, and tetrahydrofuran (121 kg) was added at an internal temperature of 30 ° C. or lower. Switching to brine cooling, sodium borohydride (7.15 kg, 1.1 M / M) was added in portions over 5 hours at an internal temperature of 0 to 10 ° C. under a nitrogen stream. After completion of the addition of sodium borohydride, the jacket was switched to cold water (4.0 ° C.) circulation and stirred overnight at an internal temperature of 0 to 10 ° C. On the next day, sodium borohydride (1.30 kg, 0.2 M / M) was added in portions at an internal temperature of 0 to 10 ° C. over 1 hour. The jacket was switched to cold water, the internal temperature was raised to 20-30 ° C. over 3 hours, and stirring was continued overnight at the internal temperature of 20-30 ° C. as it was. On the next day, the progress of the reaction was confirmed by HPLC, but the reaction was hardly proceeding, so it was cooled again and sodium borohydride (1.30 kg, 0.2 M / M) was added at an internal temperature of 0 to 10 ° C. Add in portions. After stirring for 1 hour or more at an internal temperature of 0 to 10 ° C., the jacket was switched to cold water circulation, and the temperature was raised to an internal temperature of 15 to 25 ° C. over 2 hours or more. After stirring for 1 hour or longer, it was confirmed by HPLC (Condition 1) that the reaction was complete, and the mixture was stirred overnight.
The next day, 48.7% aqueous sodium hydroxide solution (71 kg, 5 M / M) was added dropwise at an internal temperature of 50 ° C. or lower, and then water (133 L) was added dropwise at an internal temperature of 50 ° C. or lower. Hot water (50 to 80 ° C.) circulation was started, and the mixture was stirred at an internal temperature of 50 to 60 ° C. for 20 hours or longer. Then, it was confirmed by HPLC (condition 2) that the reaction was complete.
Subsequently, water (73 L) was dripped under water cooling. Switching to cold water (15 ° C.) cooling, [5- (2-aminopyridin-3-yl) isoxazol-3-yl] methanol was added at an internal temperature of 15-30 ° C. After confirming the precipitation of solids, water (218 L) was added. Subsequently, 35% hydrochloric acid (115 kg) was added dropwise at an internal temperature of 15 to 30 ° C. with brine cooling, and washed with water (3 L). After stirring at an internal temperature of 15 to 30 ° C. for 5 minutes or longer, the pH of the reaction solution was confirmed to be 4.00 to 5.00 with a pH meter, and stirred at an internal temperature of 15 to 30 ° C. for 1 hour or longer. Then, a 48.7% aqueous sodium hydroxide solution was added dropwise until the pH of the solution reached 7.00 to 8.00 (17.1 kg used) and left overnight. On the next day, stirring and decompression were started, and after confirming distillation from the condenser, circulation of warm water (40 ° C.) was started. Concentration was performed for 1 hour or more under conditions of warm water (35 to 45 ° C.), a reduced pressure of 68 cmHg or more, and an internal temperature of 30 ° C. or more. The reduced pressure was released with nitrogen, and the solid adhering to the can wall was washed with water (about 20 L). The mixture was stirred at an internal temperature of 15 to 30 ° C. for 3 hours or more and allowed to stand overnight. The next day, it was confirmed that the internal temperature was within the range of 15 to 25 ° C., and the slurry was separated into solid and liquid by a centrifuge in two steps. After each centrifugation, it was washed with water (about 200 L), centrifuged for 1 hour after running out of liquid, and the wet solid was taken out into a plastic bag. Next, the product was dried under reduced pressure in a shelf-type dryer under hot water circulation at 45 to 50 ° C. to obtain the title compound (26.57 kg, yield: 80.9%).
1 H-NMR Spectrum (DMSO-d 6 ) δ (ppm): 4.54 (2H, s), 5.57 (1H, brs), 6.25 (2H, brs), 6.71 (1H, dd, J = 4.8, 8.0 Hz) , 6.90 (1H, s), 7.90 (1H, dd, J = 1.6, 7.6Hz), 8.09 (1H, dd, J = 1.6, 4.8Hz).
HPLC condition 1 Column: YMC-Pack Pro C18 (5 μm, 150 × 4.6 mm I.D., YMC), mobile phase: acetonitrile / water / ammonium acetate = 300/700/1 to 900/100/1 (v / v / w) ).
HPLC condition 2 Column: YMC-Pack ODS-AQ (5 μm, 150 × 4.6 mm I.D., YMC), mobile phase: acetonitrile / water / 85% phosphoric acid / 1-octanesulfonic acid sodium salt = 161.3 / 838.7 /1/1.1 to 900/100/1 / 1.1 (v / v / v / w).
　参考例１４～１５は、参考例１０の別途合成法である。 Reference Examples 14 to 15 are separate synthesis methods of Reference Example 10.
［参考例１４］[５－（２－アミノピリジン－３－イル）イソキサゾール－３－イル]メタノール　シュウ酸塩の合成
Figure JPOXMLDOC01-appb-C000062
 　エチル　５－｛２－［（２，２－ジメチルプロパノイル）アミノ］ピリジン－３－イル｝イソキサゾール－３－カルボキシレート（３．１７ｇ，１０ｍｍｏｌ）、エタノール（３ｍＬ）、およびテトラヒドロフラン（１０ｍＬ）の混合物に室温で水素化ホウ素ナトリウム（０．３８ｇ，１０ｍｍｏｌ）を加え、氷冷下から室温にて終夜攪拌した。反応混合物を５等分したうちの１つに５Ｎ水酸化ナトリウム水溶液（２ｍＬ）を加え、５５℃にて終夜攪拌した。反応混合物に水を加え、メチル－ｔｅｒｔ－ブチルエーテルとテトラヒドロフランの混合液で抽出し、有機層にシュウ酸（０．１８ｇ，２ｍｍｏｌ）を加えた。析出した固体を濾過し、標記化合物（０．３９ｇ）を白色固体として得た。
1H－NMR  Spectrum  (DMSO－d6)δ(ppm):4.54(2H,s),  6.31(2H,brs),　6.72(1H,dd,J＝4.8,8.0Hz),  6.89(1H,s),　7.90(1H,dd,J＝2.0,8.0Hz),  8.09(1H,dd,J＝2.0,4.8Hz). [Reference Example 14] Synthesis of [5- (2-aminopyridin-3-yl) isoxazol-3-yl] methanol oxalate
Figure JPOXMLDOC01-appb-C000062
 Mixture of ethyl 5- {2-[(2,2-dimethylpropanoyl) amino] pyridin-3-yl} isoxazole-3-carboxylate (3.17 g, 10 mmol), ethanol (3 mL), and tetrahydrofuran (10 mL) To the mixture was added sodium borohydride (0.38 g, 10 mmol) at room temperature, and the mixture was stirred overnight at room temperature under ice cooling. A 5N aqueous sodium hydroxide solution (2 mL) was added to one of the 5 aliquots of the reaction mixture, and the mixture was stirred at 55 ° C. overnight. Water was added to the reaction mixture, extraction was performed with a mixed solution of methyl-tert-butyl ether and tetrahydrofuran, and oxalic acid (0.18 g, 2 mmol) was added to the organic layer. The precipitated solid was filtered to obtain the title compound (0.39 g) as a white solid.
1 H-NMR Spectrum (DMSO-d 6 ) δ (ppm): 4.54 (2H, s), 6.31 (2H, brs), 6.72 (1H, dd, J = 4.8, 8.0 Hz), 6.89 (1H, s) , 7.90 (1H, dd, J = 2.0,8.0Hz), 8.09 (1H, dd, J = 2.0,4.8Hz).
［参考例１５］[５－（２－アミノピリジン－３－イル）イソキサゾール－３－イル]メタノールの合成
Figure JPOXMLDOC01-appb-C000063
 　［５－（２－アミノピリジン－３－イル）イソキサゾール－３－イル］メタノール　シュウ酸塩（０．３９ｇ）および水（２ｍＬ）の混合物に室温で５Ｎ水酸化ナトリウム水溶液（０．５ｍＬ）を加え、析出した固体を濾過し、標記化合物（０．１８ｇ）を白色固体として得た。
1H－NMR  Spectrum  (DMSO－d6)δ(ppm)：4.54(2H,s),  5.57(1H,brs),　6.25(2H,brs),  6.71(1H,dd,J＝4.8,8.0Hz),  6.90(1H,s),　7.90(1H,dd,J＝1.6,7.6Hz),  8.09(1H,dd,J＝1.6,4.8Hz). Reference Example 15 Synthesis of [5- (2-aminopyridin-3-yl) isoxazol-3-yl] methanol
Figure JPOXMLDOC01-appb-C000063
 To a mixture of [5- (2-aminopyridin-3-yl) isoxazol-3-yl] methanol oxalate (0.39 g) and water (2 mL) was added 5N aqueous sodium hydroxide solution (0.5 mL) at room temperature. The precipitated solid was filtered to obtain the title compound (0.18 g) as a white solid.
1 H-NMR Spectrum (DMSO-d 6 ) δ (ppm): 4.54 (2H, s), 5.57 (1H, brs), 6.25 (2H, brs), 6.71 (1H, dd, J = 4.8, 8.0 Hz) , 6.90 (1H, s), 7.90 (1H, dd, J = 1.6, 7.6Hz), 8.09 (1H, dd, J = 1.6, 4.8Hz).
［参考例１６］３－[３－（クロロメチル）イソキサゾール－５－イル]ピリジン－２－アミンの合成
Figure JPOXMLDOC01-appb-C000064
 　［５－（２－アミノピリジン－３－イル）イソキサゾール－３－イル］メタノール（０．１９ｇ，１ｍｍｏｌ）、Ｎ，Ｎ－ジメチルアセトアミド（１ｍＬ）の混合物に、塩化チオニル（０．１５ｍＬ，２ｍｍｏｌ）、ベンゾトリアゾール（０．２６ｇ，２．２ｍｍｏｌ）およびテトラヒドロフラン（１ｍＬ）の混合物を氷冷下にて加え、室温にて３０分間攪拌した。反応混合物に水および５Ｎ水酸化ナトリウム水溶液を加えアルカリ性とした後、酢酸エチルにて抽出し、有機層を飽和食塩水で洗浄した。溶媒を減圧下留去し、標記化合物（０．２１ｇ）を淡黄色固体として得た。
1H－NMR  Spectrum  (DMSO－d6)δ(ppm):4.84(2H,s),  6.31(2H,brs),　6.72(1H,dd,J＝4.8,8.0Hz),  7.04(1H,s),　7.91(1H,dd,J＝1.6,7.6Hz),  8.11(1H,dd,J＝1.2,4.8Hz). [Reference Example 16] Synthesis of 3- [3- (chloromethyl) isoxazol-5-yl] pyridin-2-amine
Figure JPOXMLDOC01-appb-C000064
 To a mixture of [5- (2-aminopyridin-3-yl) isoxazol-3-yl] methanol (0.19 g, 1 mmol) and N, N-dimethylacetamide (1 mL), thionyl chloride (0.15 mL, 2 mmol) was added. , Benzotriazole (0.26 g, 2.2 mmol) and tetrahydrofuran (1 mL) were added under ice-cooling, and the mixture was stirred at room temperature for 30 minutes. Water and 5N aqueous sodium hydroxide solution were added to the reaction mixture to make it alkaline, followed by extraction with ethyl acetate, and the organic layer was washed with saturated brine. The solvent was distilled off under reduced pressure to obtain the title compound (0.21 g) as a pale yellow solid.
1 H-NMR Spectrum (DMSO-d 6 ) δ (ppm): 4.84 (2H, s), 6.31 (2H, brs), 6.72 (1H, dd, J = 4.8,8.0Hz), 7.04 (1H, s) , 7.91 (1H, dd, J = 1.6, 7.6Hz), 8.11 (1H, dd, J = 1.2, 4.8Hz).
［参考例１７］ジ－ｔｅｒｔ－ブチル　｛３－［３－（クロロメチル）イソキサゾール－５－イル］ピリジン－２－イル｝イミドジカーボネートの合成
Figure JPOXMLDOC01-appb-C000065
 　３－［３－（クロロメチル）イソキサゾール－５－イル］ピリジン－２－アミン（４２０ｍｇ，２．０１ｍｍｏｌ）、４－ジメチルアミノピリジン（２６．８ｍｇ，０．２２０ｍｍｏｌ）、テトラヒドロフラン（２．１ｍＬ）の混合物に、室温で、ジ－ｔｅｒｔ－ブチルジカーボネイト（９２４ｍｇ，４．２４ｍｍｏｌ）を加え撹拌した。２５時間後、反応液へ水を加えトルエンで抽出した後、有機層を５％食塩水で洗浄し、減圧下溶媒を留去し、標記化合物（８８０ｍｇ）を淡黄色油状物として得た。
1H－NMR  Spectrum  (CDCl3)δ(ppm):1.33(18H,s),　4.63(2H,s),  6.66(1H,s),　7.45(1H,dd,J＝4.8,8.0Hz),  8.30(1H,dd,J＝2.0,8.0Hz),  8.62(1H,dd,J＝2.0,4.8Hz). [Reference Example 17] Synthesis of di-tert-butyl {3- [3- (chloromethyl) isoxazol-5-yl] pyridin-2-yl} imide dicarbonate
Figure JPOXMLDOC01-appb-C000065
 Of 3- [3- (chloromethyl) isoxazol-5-yl] pyridin-2-amine (420 mg, 2.01 mmol), 4-dimethylaminopyridine (26.8 mg, 0.220 mmol), tetrahydrofuran (2.1 mL) To the mixture, di-tert-butyl dicarbonate (924 mg, 4.24 mmol) was added and stirred at room temperature. After 25 hours, water was added to the reaction mixture, and the mixture was extracted with toluene. The organic layer was washed with 5% brine, and the solvent was evaporated under reduced pressure to give the title compound (880 mg) as a pale yellow oil.
1 H-NMR Spectrum (CDCl 3 ) δ (ppm): 1.33 (18H, s), 4.63 (2H, s), 6.66 (1H, s), 7.45 (1H, dd, J = 4.8, 8.0 Hz), 8.30 (1H, dd, J = 2.0,8.0Hz), 8.62 (1H, dd, J = 2.0,4.8Hz).
　参考例１８～２１は、参考例９～１０および参考例１６～１７の別途合成法である。 Reference Examples 18 to 21 are separate synthesis methods of Reference Examples 9 to 10 and Reference Examples 16 to 17.
［参考例１８］ｔｅｒｔ－ブチル　｛３－［３－（ヒドロキシメチル）イソキサゾール－５－イル］ピリジン－２－イル｝カルバメイトの合成
Figure JPOXMLDOC01-appb-C000066
 　エチル　５－｛２－［（２，２－ジメチルプロパノイル）アミノ］ピリジン－３－イル｝イソキサゾール－３－カルボキシレート（１．５９ｇ，５ｍｍｏｌ）、ジ－ｔｅｒｔ－ブチルジカーボネイト（１．３１ｇ，６ｍｍｏｌ）、およびテトラヒドロフラン（５ｍＬ）の混合物に室温で４－ジメチルアミノピリジン（６１ｍｇ，０．５ｍｍｏｌ）を加え、室温で１時間攪拌後、６０℃で６時間攪拌した。反応混合物にエタノール（２．５ｍＬ）および水素化ホウ素ナトリウム（０．５７ｇ，１５ｍｍｏｌ）を加え、０℃で３０分間攪拌後、室温終夜攪拌した。反応混合物に水を加え、酢酸エチルで抽出し、有機層を水と飽和食塩水で洗浄した。無水硫酸マグネシウムで乾燥し、濾過後、溶媒を減圧下留去し、標記化合物（１．６０ｇ）を得た。
1H－NMR  Spectrum  (CDCl3)δ(ppm):1.47(9H,s),  4.83(2H,s),　6.63(1H,s),  7.17(1H,dd,J＝4.8,8.0Hz),  7.58(1H,s),　7.97(1H,dd,J＝2.0,8.0Hz),  8.51(1H,dd,J＝2.0,4.8Hz). [Reference Example 18] Synthesis of tert-butyl {3- [3- (hydroxymethyl) isoxazol-5-yl] pyridin-2-yl} carbamate
Figure JPOXMLDOC01-appb-C000066
 Ethyl 5- {2-[(2,2-dimethylpropanoyl) amino] pyridin-3-yl} isoxazole-3-carboxylate (1.59 g, 5 mmol), di-tert-butyl dicarbonate (1.31 g, 6-dimethyl) and tetrahydrofuran (5 mL) were added 4-dimethylaminopyridine (61 mg, 0.5 mmol) at room temperature, stirred at room temperature for 1 hour, and then stirred at 60 ° C. for 6 hours. Ethanol (2.5 mL) and sodium borohydride (0.57 g, 15 mmol) were added to the reaction mixture, and the mixture was stirred at 0 ° C. for 30 min and then overnight at room temperature. Water was added to the reaction mixture, extraction was performed with ethyl acetate, and the organic layer was washed with water and saturated brine. After drying over anhydrous magnesium sulfate and filtration, the solvent was evaporated under reduced pressure to obtain the title compound (1.60 g).
1 H-NMR Spectrum (CDCl3) δ (ppm): 1.47 (9H, s), 4.83 (2H, s), 6.63 (1H, s), 7.17 (1H, dd, J = 4.8, 8.0 Hz), 7.58 ( 1H, s), 7.97 (1H, dd, J = 2.0, 8.0Hz), 8.51 (1H, dd, J = 2.0, 4.8Hz).
［参考例１９］ｔｅｒｔ－ブチル　｛３－［３－（クロロメチル）イソキサゾール－５－イル］ピリジン－２－イル｝カルバメートの合成
Figure JPOXMLDOC01-appb-C000067
 　窒素雰囲気下、ベンゾトリアゾール（３．５５ｇ，２９．５ｍｍｏｌ）をＮ，Ｎ－ジメチルアセトアミド（１０ｍＬ）に溶解し、氷水冷下、塩化チオニル（２．０６ｍＬ，２６．８ｍｍｏｌ）を滴下し、塩化チオニル－ベンゾトリアゾール（１：１．１）のＮ，Ｎ－ジメチルアセトアミド溶液を調製した。
　窒素雰囲気下、ｔｅｒｔ－ブチル　｛３－［３－（ヒドロキシメチル）イソキサゾール－５－イル］ピリジン－２－イル｝カルバメート（７８１ｍｇ，２．６８ｍｍｏｌ）をＮ，Ｎ－ジメチルアセトアミド（２．７ｍＬ）に溶解し、氷水冷下、上記した塩化チオニル－ベンゾトリアゾール（１：１．１）のＮ，Ｎ－ジメチルアセトアミド溶液（６ｍＬ，１４．４ｍｍｏｌ）を滴下し、同温で１時間撹拌した後、室温で撹拌した。１時間２０分後、氷水冷下、塩化チオニル－ベンゾトリアゾール（１：１．１）のＮ，Ｎ－ジメチルアセトアミド溶液（２．２ｍＬ，５．１２ｍｍｏｌ）を滴下し、室温で１時間撹拌した。氷水冷下、反応液へ１Ｎ水酸化ナトリウム水溶液とｔｅｒｔ－ブチルメチルエーテルを加え、塩基性とした後、抽出した。有機層を０．５Ｎ水酸化ナトリウム水溶液、５％食塩水で順次洗浄し、無水硫酸マグネシウムで乾燥した後、減圧下溶媒を留去し、標記化合物の粗体（９５３ｍｇ）を淡黄色油状物として得た。
1H－NMR  Spectrum  (CDCl3)δ(ppm):1.47(9H,s),  4.65(2H,s),　6.67(1H,s),  7.20(1H,dd,J＝4.8,8.0Hz),  7.44(1H,brs),　8.01(1H,dd,J＝2.0,8.0Hz),  8.52(1H,dd,J＝2.0,4.8Hz). [Reference Example 19] Synthesis of tert-butyl {3- [3- (chloromethyl) isoxazol-5-yl] pyridin-2-yl} carbamate
Figure JPOXMLDOC01-appb-C000067
 Under nitrogen atmosphere, benzotriazole (3.55 g, 29.5 mmol) was dissolved in N, N-dimethylacetamide (10 mL), and thionyl chloride (2.06 mL, 26.8 mmol) was added dropwise under ice water cooling. -A solution of benzotriazole (1: 1.1) in N, N-dimethylacetamide was prepared.
Under a nitrogen atmosphere, tert-butyl {3- [3- (hydroxymethyl) isoxazol-5-yl] pyridin-2-yl} carbamate (781 mg, 2.68 mmol) was added to N, N-dimethylacetamide (2.7 mL). Dissolve the solution, and add a solution of thionyl chloride-benzotriazole (1: 1.1) in N, N-dimethylacetamide (6 mL, 14.4 mmol) dropwise under ice-water cooling and stir at the same temperature for 1 hour. Stir with. After 1 hour and 20 minutes, a solution of thionyl chloride-benzotriazole (1: 1.1) in N, N-dimethylacetamide (2.2 mL, 5.12 mmol) was added dropwise with ice water cooling, and the mixture was stirred at room temperature for 1 hour. Under cooling with ice water, 1N aqueous sodium hydroxide solution and tert-butyl methyl ether were added to the reaction solution to make it basic, and then extracted. The organic layer was washed successively with 0.5N aqueous sodium hydroxide solution and 5% brine and dried over anhydrous magnesium sulfate, and the solvent was evaporated under reduced pressure to give the crude title compound (953 mg) as a pale yellow oil. Obtained.
1 H-NMR Spectrum (CDCl 3 ) δ (ppm): 1.47 (9H, s), 4.65 (2H, s), 6.67 (1H, s), 7.20 (1H, dd, J = 4.8, 8.0 Hz), 7.44 (1H, brs), 8.01 (1H, dd, J = 2.0,8.0Hz), 8.52 (1H, dd, J = 2.0,4.8Hz).
［参考例２０］ジ－ｔｅｒｔ－ブチル　｛３－［３－（クロロメチル）イソキサゾール－５－イル］ピリジン－２－イル｝イミドジカーボネートの合成
Figure JPOXMLDOC01-appb-C000068
 　ｔｅｒｔ－ブチル　｛３－［３－（クロロメチル）イソキサゾール－５－イル］ピリジン－２－イル｝カルバメートの粗体（１．１３ｇ，３．１７ｍｍｏｌ）をテトラヒドロフラン（７．０ｍＬ）に溶解し、氷水冷下、ジ－ｔｅｒｔ－ブチルジカーボネイト（７６１ｍｇ，３．４９ｍｍｏｌ）を加えＴＨＦ（３．０ｍＬ）で洗い込んだ。次いで、４－ジメチルアミノピリジン（３９．１ｍｇ，０．３１７ｍｍｏｌ）を加えた後、室温で撹拌した。５時間後、氷水冷下、反応液へ酢酸エチルと５％食塩水を加え、抽出した。有機層を５％食塩水で洗浄し、無水硫酸マグネシウムで乾燥した後、減圧下溶媒を留去した。残渣をシリカゲルカラムクロマトグラフィーで精製し、標記化合物（１．１４ｇ）を淡黄色固体として得た。
1H－NMR  Spectrum  (CDCl3)δ(ppm)：1.33(18H,s),　4.63(2H,s),  6.66(1H,s),　7.45(1H,dd,J＝4.8,8.0Hz),  8.30(1H,dd,J＝2.0,8.0Hz),  8.62(1H,dd,J＝2.0,4.8Hz). [Reference Example 20] Synthesis of di-tert-butyl {3- [3- (chloromethyl) isoxazol-5-yl] pyridin-2-yl} imide dicarbonate
Figure JPOXMLDOC01-appb-C000068
 A crude product of tert-butyl {3- [3- (chloromethyl) isoxazol-5-yl] pyridin-2-yl} carbamate (1.13 g, 3.17 mmol) was dissolved in tetrahydrofuran (7.0 mL) and iced. Under water cooling, di-tert-butyl dicarbonate (761 mg, 3.49 mmol) was added and washed with THF (3.0 mL). Subsequently, 4-dimethylaminopyridine (39.1 mg, 0.317 mmol) was added, followed by stirring at room temperature. After 5 hours, the reaction mixture was extracted with ethyl acetate and 5% brine under ice-water cooling. The organic layer was washed with 5% brine and dried over anhydrous magnesium sulfate, and then the solvent was distilled off under reduced pressure. The residue was purified by silica gel column chromatography to obtain the title compound (1.14 g) as a pale yellow solid.
1 H-NMR Spectrum (CDCl 3 ) δ (ppm): 1.33 (18H, s), 4.63 (2H, s), 6.66 (1H, s), 7.45 (1H, dd, J = 4.8, 8.0 Hz), 8.30 (1H, dd, J = 2.0,8.0Hz), 8.62 (1H, dd, J = 2.0,4.8Hz).
［参考例２１］ジ－ｔｅｒｔ－ブチル　｛３－［３－（クロロメチル）イソキサゾール－５－イル］ピリジン－２－イル｝イミドジカーボネートの合成
Figure JPOXMLDOC01-appb-C000069
 　窒素気流下、５００Ｌ反応缶１に［５－（２－アミノピリジン－３－イル）イソキサゾール－３－イル］メタノール（２６．００ｋｇ，１３６．０ｍｏｌ）と１，３－ジメチル－２－イミダゾリジノン（１４３ｋｇ，５．５ｗ／ｗ，洗い込み用に一部取分け）を加え、攪拌を開始した。内温３５～４５℃で１時間以上攪拌し、［５－（２－アミノピリジン－３－イル）イソキサゾール－３－イル］メタノールの溶解後、冷却した．内温５～２５℃で塩化チオニル（１９．４０ｋｇ，１６３．１ｍｏｌ，１．２Ｍ／Ｍ）を滴下した。滴下終了後、取分けた１，３－ジメチル－２－イミダゾリジノンで塩化チオニルを洗い込み、内温５～２５℃で１２時間以上攪拌した。ＨＰＬＣ分析で反応終了を確認した後、内温０～２５℃で約３６％水酸化ナトリウム水溶液（４８％水酸化ナトリウム水溶液（１５．９ｋｇ，水酸化ナトリウムとして１９０．８　ｍｏｌ，１．４Ｍ／Ｍ）と水（５．３ｋｇ，０．２ｗ／ｗ）の混液）を滴下し、次いで内温１５～３５℃で酢酸エチル（１６４ｋｇ，６．３１ｗ／ｗ）および水（７４．２ｋｇ，２．８５ｗ／ｗ）を滴下した。さらに、内温０～２５℃で約８％水酸化ナトリウム水溶液（４８％水酸化ナトリウム溶液（１３．６ｋｇ，水酸化ナトリウムとして１６３．２ｍｏｌ，１．２０Ｍ／Ｍ）と水（６８．０ｋｇ，２．６ｗ／ｗ）の混液）を滴下し、内温を１５～３０℃に調整した後、同温度範囲で３０分以上攪拌し、３０分以上静置した。下層と上層を別々に取出し、それぞれ１／２重量ずつを５００Ｌ反応缶１及び５００Ｌ反応缶２に加えた。
　５００Ｌ反応缶１の後処理は以下のように実施した。攪拌を開始して水（５２ｋｇ，２ｗ／ｗ）を加えた後、内温０～２５℃で約８％水酸化ナトリウム水溶液（４８％水酸化ナトリウム水溶液（１１．３ｋｇ，水酸化ナトリウムとして１３５．６ｍｏｌ，１．０Ｍ／Ｍ）と水（５６．５ｋｇ，２．１７ｗ／ｗ）の混液）を少しずつ滴下し、下層のｐＨを７．００～８．５０（実測値：ｐＨ７．８４）に調整した。この時、約８％水酸化ナトリウム水溶液は３５．５５ｋｇ使用した。続いて、内温を１５～３０℃に調整し、３０分以上攪拌後、終夜静置した。翌日、ｐＨがｐＨ７．５９であることを再度確認した後、上層と下層をそれぞれ分取し、下層のみを５００Ｌ反応缶１に戻した後、酢酸エチル（８２ｋｇ，３．１５ｗ／ｗ）を加えた。内温１５～３０℃で５分攪拌後、３０分以上静置し、下層（ｐＨ７．５５）を除去した。缶に残した上層に分取しておいた上層および５％食塩水（食塩（３．３ｋｇ，０．１３ｗ／ｗ）と水（６１８ｋｇ，２．３８ｗ／ｗ）の混液）を加え、内温１５～３０℃で５分攪拌後、３０分以上静置して下層（ｐＨ８．２３）を除去した。さらに、水（６５ｋｇ，２．５ｗ／ｗ）を加えて、内温１５～３０℃で５分攪拌後、終夜静置して下層（ｐＨ７．０４）を除去した。
　５００Ｌ反応缶２の後処理は、５００Ｌ反応缶１の操作と並行して同じ作業を実施した。
　次に、５００Ｌ反応缶２の上層を５００Ｌ反応缶１に移送し、温水４５～５５℃、減圧度－０．０７０～－０．０８５ＭＰａで内容液が約２００Ｌとなるまで減圧濃縮した。ここに酢酸エチル（１４１ｋｇ，５．４２ｗ／ｗ）を加えて、再び同条件で減圧濃縮した。この操作をさらに２回繰り返した後、４度目の酢酸エチル（１４１ｋｇ，５．４２ｗ／ｗ）を添加する前後でのＨＰＬＣ分析により内容液の３－[３－（クロロメチル）イソキサゾール－５－イル]ピリジン－２－アミン含量を確認し、内溶液中の３－[３－（クロロメチル）イソキサゾール－５－イル]ピリジン－２－アミン含有量（２３．３５ｋｇ，１１１．４ｍｏｌ）とその収率（８１．９％）を算出した。続いて、もう一度同じ条件で３－[３－（クロロメチル）イソキサゾール－５－イル]ピリジン－２－アミン含量が１０．０～１３．０％になるまで減圧濃縮を行い、３－[３－（クロロメチル）イソキサゾール－５－イル]ピリジン－２－アミンの酢酸エチル溶液を得た。
　窒素気流下，５００Ｌ反応缶１内の３－[３－（クロロメチル）イソキサゾール－５－イル]ピリジン－２－アミンの酢酸エチル溶液（前工程で得た全量，２３．３５ｋｇ（１１１．４ｍｏｌ）を含有）を攪拌し、内温１５～２５℃でジ－ｔｅｒｔ－ブチルジカーボネイト（５３．４７ｋｇ，２４５．０ｍｏｌ，２．２Ｍ／Ｍ）を加え、酢酸エチル（２ｋｇ）で洗い込んだ。ここに、あらかじめ調製した４－ジメチルアミノピリジンの酢酸エチル溶液（４－ジメチルアミノピリジン（０．４０９ｋｇ，３．３５ｍｏｌ，０．０３Ｍ／Ｍ）と酢酸エチル（８ｋｇ）の混液）を加え、酢酸エチル（１ｋｇ）で洗い込んだ後、内温１０～３０℃で２２時間以上攪拌した。ＨＰＬＣ分析により反応の終了を確認した後、１，３－ジメチル－２－イミダゾリジノン（５０ｋｇ，２．１２ｗ／ｗ）を加えた。４５～５５℃の温水循環下、減圧度－０．０９２ＭＰａ以上かつ液留出が弱まるまで減圧濃縮し、ＧＣ分析により酢酸エチル含量が７．０％であることを確認後、内温３０℃以下まで冷却し、終夜静置した。翌日、濃縮残渣にメタノール（１１１ｋｇ，４．７４ｗ／ｗ）を加えて１０分以上攪拌し、固体が析出していないことを確認後、溶液を２分割した。次に２分割した溶液を５００Ｌ反応缶１及び２にそれぞれ加え、それぞれメタノール（各９ｋｇ，各０．４ｗ／ｗ）で洗い込んだ。この際、２分割する前の溶液（２２５．６５ｋｇ）をＨＰＬＣ分析した結果、目的のジ－ｔｅｒｔ－ブチル　｛３－［３－（クロロメチル）イソキサゾール－５－イル］ピリジン－２－イル｝イミドジカーボネート含量は１９．３７％、含まれているジ－ｔｅｒｔ－ブチル　｛３－［３－（クロロメチル）イソキサゾール－５－イル］ピリジン－２－イル｝イミドジカーボネート重量は４３．７１ｋｇ（１０６．６ｍｏｌ，収率：９５．７％）であった。
　５００Ｌ反応缶１について、以下のように処理した。攪拌を開始後、内温３５～４５℃で水（３５ｋｇ，１．５ｗ／ｗ）を３０分以上かけて滴下し、内温３５～４０℃でジ－ｔｅｒｔ－ブチル　｛３－［３－（クロロメチル）イソキサゾール－５－イル］ピリジン－２－イル｝イミドジカーボネート（０．０１０ｋｇ）を加えた。内温３５～４０℃で３０分以上攪拌後、固体の析出を確認し、さらに同温度範囲で１時間以上攪拌した。続いて、内温３５～４５℃で水（３５ｋｇを３回，各１．５ｗ／ｗ）をそれぞれ３０分以上かけて滴下した後、３時間以上かけて内温５～１５℃まで冷却し、同温度範囲で１２時間以上攪拌した。遠心分離機で２回に分けて固液分離し、含水メタノール（メタノール（１回につき７ｋｇ，０．３ｗ／ｗ）と水（１回につき２７ｋｇ，１．１４ｗ／ｗ）の混液）で洗浄した。洗浄終了後、３０分以上遠心分離を行い、標記化合物のｗｅｔ固体（２５．８０ｋｇ）を得た。このｗｅｔ固体を混合型真空乾燥機に投入し、外温４５～５５℃で２４時間以上真空乾燥し、標記化合物（２１．０９ｋｇ）を淡黄色固体として得た。
　５００Ｌ反応缶２について、上記と並行して同じ操作を行い、標記化合物（２１．２２ｋｇ）を淡黄色固体として得た。
　以上より、標記化合物（４２．３１ｋｇ，収率：９２．７％）を得た。
1H－NMR  Spectrum  (CDCl3)δ(ppm)：1.33(18H,s),　4.63(2H,s),  6.66(1H,s),　7.45(1H,dd,J＝4.8,8.0Hz),  8.30(1H,dd,J＝2.0,8.0Hz),  8.62(1H,dd,J＝2.0,4.8Hz).
ＨＰＬＣ条件　カラム：YMC-Pack　Pro  C18　(5μm,　150x4.6mmI.D.,　YMC)，移動相：アセトニトリル／水／酢酸アンモニウム=３００／７００／１～９００／１００／１（ｖ／ｖ／ｗ）。
ＧＣ条件　カラム：DB-624　(30m,  0.53mmI.D.,　Film　3μm,　Agilent)。 [Reference Example 21] Synthesis of di-tert-butyl {3- [3- (chloromethyl) isoxazol-5-yl] pyridin-2-yl} imide dicarbonate
Figure JPOXMLDOC01-appb-C000069
 [5- (2-Aminopyridin-3-yl) isoxazol-3-yl] methanol (26.00 kg, 136.0 mol) and 1,3-dimethyl-2-imidazolidinone were placed in a 500 L reactor 1 under a nitrogen stream. (143 kg, 5.5 w / w, partly for washing) was added and stirring was started. The mixture was stirred at an internal temperature of 35 to 45 ° C. for 1 hour or longer and dissolved after dissolving [5- (2-aminopyridin-3-yl) isoxazol-3-yl] methanol. Thionyl chloride (19.40 kg, 163.1 mol, 1.2 M / M) was added dropwise at an internal temperature of 5 to 25 ° C. After completion of the dropwise addition, thionyl chloride was washed with the separated 1,3-dimethyl-2-imidazolidinone and stirred at an internal temperature of 5 to 25 ° C. for 12 hours or more. After confirming the completion of the reaction by HPLC analysis, about 36% sodium hydroxide aqueous solution (48% sodium hydroxide aqueous solution (15.9 kg, 190.8 mol as sodium hydroxide, 1.4 M / M) at an internal temperature of 0 to 25 ° C. ) And water (5.3 kg, 0.2 w / w) were added dropwise, and then at an internal temperature of 15 to 35 ° C., ethyl acetate (164 kg, 6.31 w / w) and water (74.2 kg, 2.85 w) / W) was added dropwise. Further, at an internal temperature of 0 to 25 ° C., about 8% sodium hydroxide aqueous solution (48% sodium hydroxide solution (13.6 kg, 163.2 mol as sodium hydroxide, 1.20 M / M) and water (68.0 kg, 2 6 w / w)) was added dropwise and the internal temperature was adjusted to 15 to 30 ° C., and then stirred for 30 minutes or more in the same temperature range, and allowed to stand for 30 minutes or more. The lower layer and the upper layer were taken out separately, and 1/2 weight each was added to the 500 L reactor 1 and the 500 L reactor 2.
The post-treatment of the 500 L reactor 1 was performed as follows. Stirring was started and water (52 kg, 2 w / w) was added, followed by an about 8% aqueous sodium hydroxide solution (48% aqueous sodium hydroxide solution (11.3 kg, 135. 6 mol, 1.0 M / M) and water (mixed solution of 56.5 kg, 2.17 w / w) was added dropwise little by little, and the pH of the lower layer was adjusted to 7.00-8.50 (actual value: pH 7.84). It was adjusted. At this time, 35.55 kg of about 8% aqueous sodium hydroxide solution was used. Subsequently, the internal temperature was adjusted to 15 to 30 ° C., stirred for 30 minutes or more, and allowed to stand overnight. The next day, after confirming again that the pH is pH 7.59, the upper layer and the lower layer were separated, only the lower layer was returned to the 500 L reactor 1, and then ethyl acetate (82 kg, 3.15 w / w) was added. It was. After stirring for 5 minutes at an internal temperature of 15 to 30 ° C., the mixture was allowed to stand for 30 minutes or more to remove the lower layer (pH 7.55). Add the upper layer and 5% saline (mixed solution of salt (3.3 kg, 0.13 w / w) and water (618 kg, 2.38 w / w)) separated into the upper layer left in the can, After stirring at 15-30 ° C. for 5 minutes, the mixture was allowed to stand for 30 minutes or longer to remove the lower layer (pH 8.23). Further, water (65 kg, 2.5 w / w) was added, and the mixture was stirred at an internal temperature of 15 to 30 ° C. for 5 minutes and then allowed to stand overnight to remove the lower layer (pH 7.04).
In the post-treatment of the 500 L reactor 2, the same operation was performed in parallel with the operation of the 500 L reactor 1.
Next, the upper layer of the 500 L reactor 2 was transferred to the 500 L reactor 1 and concentrated under reduced pressure at a temperature of 45 to 55 ° C. and a degree of vacuum of −0.070 to −0.085 MPa until the content liquid reached about 200 L. Ethyl acetate (141 kg, 5.42 w / w) was added thereto, and the mixture was again concentrated under reduced pressure under the same conditions. After repeating this operation two more times, the content of 3- [3- (chloromethyl) isoxazol-5-yl was analyzed by HPLC analysis before and after the fourth addition of ethyl acetate (141 kg, 5.42 w / w). ] Pyridin-2-amine content was confirmed, and the content of 3- [3- (chloromethyl) isoxazol-5-yl] pyridin-2-amine in the inner solution (23.35 kg, 111.4 mol) and its yield (81.9%) was calculated. Subsequently, the solution was concentrated again under reduced pressure until the 3- [3- (chloromethyl) isoxazol-5-yl] pyridin-2-amine content was 10.0-13.0% under the same conditions. An ethyl acetate solution of (chloromethyl) isoxazol-5-yl] pyridin-2-amine was obtained.
Ethyl acetate solution of 3- [3- (chloromethyl) isoxazol-5-yl] pyridin-2-amine in a 500 L reactor 1 under a nitrogen stream (total amount obtained in the previous step, 23.35 kg (111.4 mol) Was added, and di-tert-butyl dicarbonate (53.47 kg, 245.0 mol, 2.2 M / M) was added at an internal temperature of 15 to 25 ° C., followed by washing with ethyl acetate (2 kg). To this was added a previously prepared ethyl acetate solution of 4-dimethylaminopyridine (mixture of 4-dimethylaminopyridine (0.409 kg, 3.35 mol, 0.03 M / M) and ethyl acetate (8 kg)), and ethyl acetate was added. After washing with (1 kg), the mixture was stirred at an internal temperature of 10 to 30 ° C. for 22 hours or longer. After confirming the completion of the reaction by HPLC analysis, 1,3-dimethyl-2-imidazolidinone (50 kg, 2.12 w / w) was added. Concentrate in vacuo under a hot water circulation of 45 to 55 ° C until the degree of vacuum is -0.092 MPa or more and the liquid distillation is weakened. After confirming that the ethyl acetate content is 7.0% by GC analysis, the internal temperature is 30 ° C or less. The solution was cooled down to stand overnight. On the next day, methanol (111 kg, 4.74 w / w) was added to the concentrated residue and stirred for 10 minutes or more. After confirming that no solid was precipitated, the solution was divided into two parts. Next, the divided solution was added to 500 L reactors 1 and 2, respectively, and washed with methanol (9 kg each, 0.4 w / w each). At this time, as a result of HPLC analysis of the solution (225.65 kg) before splitting, the desired di-tert-butyl {3- [3- (chloromethyl) isoxazol-5-yl] pyridin-2-yl} imide The dicarbonate content was 19.37% and the contained di-tert-butyl {3- [3- (chloromethyl) isoxazol-5-yl] pyridin-2-yl} imide dicarbonate weighed 43.71 kg (106 0.6 mol, yield: 95.7%).
About 500L reaction can 1, it processed as follows. After starting stirring, water (35 kg, 1.5 w / w) was added dropwise at an internal temperature of 35 to 45 ° C. over 30 minutes, and di-tert-butyl {3- [3- ( Chloromethyl) isoxazol-5-yl] pyridin-2-yl} imide dicarbonate (0.010 kg) was added. After stirring at an internal temperature of 35 to 40 ° C. for 30 minutes or longer, solid precipitation was confirmed, and the mixture was further stirred at the same temperature range for 1 hour or longer. Subsequently, water (35 kg three times, 1.5 w / w each) was added dropwise over 30 minutes at an internal temperature of 35 to 45 ° C., and then cooled to an internal temperature of 5 to 15 ° C. over 3 hours. The mixture was stirred for 12 hours or more in the same temperature range. Solid-liquid separation was carried out in two portions with a centrifuge and washed with hydrous methanol (mixture of methanol (7 kg, 0.3 w / w) and water (27 kg, 1.14 w / w) at a time). . After washing, the mixture was centrifuged for 30 minutes or more to obtain a wet solid (25.80 kg) of the title compound. This wet solid was put into a mixed vacuum dryer and vacuum-dried at an external temperature of 45 to 55 ° C. for 24 hours or more to obtain the title compound (21.09 kg) as a pale yellow solid.
For 500 L reactor 2, the same operation was carried out in parallel with the above to obtain the title compound (21.22 kg) as a pale yellow solid.
From the above, the title compound (42.31 kg, yield: 92.7%) was obtained.
1 H-NMR Spectrum (CDCl 3 ) δ (ppm): 1.33 (18H, s), 4.63 (2H, s), 6.66 (1H, s), 7.45 (1H, dd, J = 4.8, 8.0 Hz), 8.30 (1H, dd, J = 2.0,8.0Hz), 8.62 (1H, dd, J = 2.0,4.8Hz).
HPLC conditions Column: YMC-Pack Pro C18 (5 μm, 150 × 4.6 mm I.D., YMC), mobile phase: acetonitrile / water / ammonium acetate = 300/700/1 to 900/100/1 (v / v / w) .
GC conditions Column: DB-624 (30 m, 0.53 mm I.D., Film 3 μm, Agilent).
［参考例２２］エチル　５－｛２－［（２、２－ジメチルプロポキシカルボニル）アミノ］ピリジン－３－イル｝イソキサゾール－３－カルボキシレート
Figure JPOXMLDOC01-appb-C000070
 　国際公開第０８／１３６２７９号パンフレットの明細書製造例３－３－１に記載の方法で合成した４－メチレン－２－オキソ－４Ｈ－ピリド［２，３－ｄ］［１，３］オキサジン－１－カルボキシリック　アシッド　ｔｅｒｔ－ブチル　エステル（２．７１ｇ、１０．３７ｍｍｏｌ）、トリエチルアミン（４．２mＬ、３０ｍｍｏｌ）、およびテトラヒドロフラン（３０ｍL）の混合物に０℃でエチル　２－クロロ－２－（ヒドロキシイミノ）アセテート（４．５ｇ、３０ｍｍｏｌ）を２時間かけて加えた後、室温で１４時間攪拌した。反応混合物に水を加え、酢酸エチルで抽出し、有機層を水と飽和食塩水で洗浄した。硫酸マグネシウムで乾燥し、ろ過後、溶媒を減圧下留去した。残渣をｎ－ヘキサンと酢酸エチルが１：１の混合液で懸濁して洗浄し、標記化合物（１．５６ｇ）を得た。
1H－NMR  Spectrum  (CDCl3)δ(ppm):1.44(3H,t,J=6.8Hz),  1.46(9H,s),　4.47(4H,q,J＝7.2Hz),  6.95(1H,s),　7.22(1H,dd,J＝4.8,8.0Hz),  7.42(1H,bs),  8.05(1H,dd,J＝2.0,8.0Hz),  8.52(1H,dd,J＝2.0,4.8Hz). [Reference Example 22] Ethyl 5- {2-[(2,2-dimethylpropoxycarbonyl) amino] pyridin-3-yl} isoxazole-3-carboxylate
Figure JPOXMLDOC01-appb-C000070
 4-methylene-2-oxo-4H-pyrido [2,3-d] [1,3] oxazine synthesized by the method described in the specification Preparation Example 3-3-1 of WO08 / 136279 Ethyl 2-chloro-2- (hydroxyimino) at 0 ° C. in a mixture of 1-carboxylic acid tert-butyl ester (2.71 g, 10.37 mmol), triethylamine (4.2 mL, 30 mmol), and tetrahydrofuran (30 mL) Acetate (4.5 g, 30 mmol) was added over 2 hours and then stirred at room temperature for 14 hours. Water was added to the reaction mixture, extraction was performed with ethyl acetate, and the organic layer was washed with water and saturated brine. After drying over magnesium sulfate and filtration, the solvent was distilled off under reduced pressure. The residue was suspended and washed with a 1: 1 mixture of n-hexane and ethyl acetate to obtain the title compound (1.56 g).
1 H-NMR Spectrum (CDCl3) δ (ppm): 1.44 (3H, t, J = 6.8Hz), 1.46 (9H, s), 4.47 (4H, q, J = 7.2Hz), 6.95 (1H, s) , 7.22 (1H, dd, J = 4.8,8.0Hz), 7.42 (1H, bs), 8.05 (1H, dd, J = 2.0,8.0Hz), 8.52 (1H, dd, J = 2.0,4.8Hz).
［参考例２３］エチル　５－｛２－［ビス（２、２－ジメチルプロポキシカルボニル）アミノ］ピリジン－３－イル｝イソキサゾール－３－カルボキシレート
Figure JPOXMLDOC01-appb-C000071
 　エチル　５－｛２－［（２、２－ジメチルプロポキシカルボニル）アミノ］ピリジン－３－イル｝イソキサゾール－３－カルボキシレート（１．４６ｇ、４．３８ｍｍｏｌ）、二炭酸ジ－ｔｅｒｔ－ブチル（１．４６ｇ、６．６９ｍｍｏｌ）、およびテトラヒドロフラン（２５ｍＬ）の混合物に室温で４－ジメチルアミノピリジン（３０ｍｇ、０．２５ｍｍｏｌ）を加え、室温で１４時間攪拌した。反応混合物に水を加え、酢酸エチルで抽出し、有機層を水と飽和食塩水で洗浄した。硫酸マグネシウムで乾燥し、ろ過後、溶媒を減圧下留去し、残渣をシリカゲルカラムクロマトグラフィー（ヘキサン：酢酸エチル＝3：１ついで１：１）にて精製し、標記化合物（１．９６ｇ）を得た。
1H－NMR  Spectrum  (CDCl3)δ(ppm):1.36(18H,s),　1.46(3H,t,J＝6.8Hz),  4.47(4H,q,J＝6.8Hz),  6.93(1H,s),　7.46(1H,dd,J＝4.8,7.6Hz),  8.29(1H,d,J＝7.6Hz),  8.64(1H,d,J＝4.8Hz). [Reference Example 23] Ethyl 5- {2- [bis (2,2-dimethylpropoxycarbonyl) amino] pyridin-3-yl} isoxazole-3-carboxylate
Figure JPOXMLDOC01-appb-C000071
 Ethyl 5- {2-[(2,2-dimethylpropoxycarbonyl) amino] pyridin-3-yl} isoxazole-3-carboxylate (1.46 g, 4.38 mmol), di-tert-butyl dicarbonate (1. 4-Dimethylaminopyridine (30 mg, 0.25 mmol) was added to a mixture of 46 g, 6.69 mmol) and tetrahydrofuran (25 mL) at room temperature, and the mixture was stirred at room temperature for 14 hours. Water was added to the reaction mixture, extraction was performed with ethyl acetate, and the organic layer was washed with water and saturated brine. After drying over magnesium sulfate and filtration, the solvent was distilled off under reduced pressure, and the residue was purified by silica gel column chromatography (hexane: ethyl acetate = 3: 1 then 1: 1) to give the title compound (1.96 g). Obtained.
1 H-NMR Spectrum (CDCl3) δ (ppm): 1.36 (18H, s), 1.46 (3H, t, J = 6.8Hz), 4.47 (4H, q, J = 6.8Hz), 6.93 (1H, s) , 7.46 (1H, dd, J = 4.8, 7.6Hz), 8.29 (1H, d, J = 7.6Hz), 8.64 (1H, d, J = 4.8Hz).
［参考例２４］ジ－ｔｅｒｔ－ブチル　｛３－［３－（ヒドロキシメチル）イソキサゾール－５－イル］ピリジン－２－イル｝イミドジカーボネート
Figure JPOXMLDOC01-appb-C000072
 　エチル　５－｛２－［ビス（２，２－ジメチルプロポキシカルボニル）アミノ］ピリジン－３－イル｝イソキサゾール－３－カルボキシレート（１．７３ｇ，４ｍｍｏｌ）、エタノール（５ｍＬ）、およびテトラヒドロフラン（５ｍＬ）の混合物に０℃で水素化ホウ素ナトリウム（０．１５ｇ，４ｍｍｏｌ）を加え、室温で１時間攪拌した。さらに水素化ホウ素ナトリウム（０．１５ｇ，４ｍｍｏｌ）を加え、室温で３時間攪拌した。反応混合物に水を加え、酢酸エチルで抽出し、有機層を水と飽和食塩水で洗浄した。無水硫酸マグネシウムで乾燥し、濾過後、溶媒を減圧下留去した。残渣にｎ－ヘキサン－酢酸エチル（１：１）の混合液を加えて懸濁撹拌した後、濾過し、標記化合物（１．０２ｇ）を得た。
1H-NMR  Spectrum  (CDCl3)δ(ppm):1.33(18H,s),　4.81(2H,s),  6.60(1H,s),　7.43(1H,dd,J＝4.8,8.0Hz),  8.27(1H,dd,J＝2.0,8.0Hz),  8.60(1H,dd,J＝2.0,4.8Hz). [Reference Example 24] Di-tert-butyl {3- [3- (hydroxymethyl) isoxazol-5-yl] pyridin-2-yl} imide dicarbonate
Figure JPOXMLDOC01-appb-C000072
 Of ethyl 5- {2- [bis (2,2-dimethylpropoxycarbonyl) amino] pyridin-3-yl} isoxazole-3-carboxylate (1.73 g, 4 mmol), ethanol (5 mL), and tetrahydrofuran (5 mL) To the mixture was added sodium borohydride (0.15 g, 4 mmol) at 0 ° C., and the mixture was stirred at room temperature for 1 hour. Further, sodium borohydride (0.15 g, 4 mmol) was added, and the mixture was stirred at room temperature for 3 hours. Water was added to the reaction mixture, extraction was performed with ethyl acetate, and the organic layer was washed with water and saturated brine. After drying over anhydrous magnesium sulfate and filtration, the solvent was distilled off under reduced pressure. A mixed solution of n-hexane-ethyl acetate (1: 1) was added to the residue, and the mixture was suspended and stirred, followed by filtration to obtain the title compound (1.02 g).
1 H-NMR Spectrum (CDCl 3 ) δ (ppm): 1.33 (18H, s), 4.81 (2H, s), 6.60 (1H, s), 7.43 (1H, dd, J = 4.8, 8.0 Hz), 8.27 (1H, dd, J = 2.0,8.0Hz), 8.60 (1H, dd, J = 2.0,4.8Hz).
［参考例２５］ジ－ｔｅｒｔ－ブチル　｛３－［３－（ブロモメチル）イソキサゾール－５－イル］ピリジン－２－イル｝イミドジカーボネート
Figure JPOXMLDOC01-appb-C000073
 　ジ－ｔｅｒｔ－ブチル　｛３－［３－（ヒドロキシメチル）イソキサゾール－５－イル］ピリジン－２－イル｝イミドジカーボネート（０．７８ｇ，２ｍｍｏｌ）、トリエチルアミン（１．９５ｍＬ，１４ｍｍｏｌ）、および１，２－ジメトキシエタン（１０ｍＬ）の混合物に０℃で三臭化リン（０．３７ｍＬ，４ｍｍｏｌ）を滴下し、室温で２時間攪拌後、５０℃で３０分間攪拌した。反応混合物を０℃に冷却後、水を加え、酢酸エチルで抽出し、有機層を飽和食塩水で洗浄した。無水硫酸マグネシウムで乾燥し、濾過後、溶媒を減圧下留去し、残渣をシリカゲルカラムクロマトグラフィーにて精製し、標記化合物（０．１４ｇ）を得た。
1H－NMR  Spectrum  (CDCl3)δ(ppm):1.33(18H,s),　4.45(2H,s),  6.63(1H,s),　7.43(1H,dd,J＝4.8,8.0Hz),  8.28(1H,dd,J＝2.0,8.0Hz),  8.61(1H,dd,J＝2.0,4.8Hz). [Reference Example 25] Di-tert-butyl {3- [3- (bromomethyl) isoxazol-5-yl] pyridin-2-yl} imide dicarbonate
Figure JPOXMLDOC01-appb-C000073
 Di-tert-butyl {3- [3- (hydroxymethyl) isoxazol-5-yl] pyridin-2-yl} imide dicarbonate (0.78 g, 2 mmol), triethylamine (1.95 mL, 14 mmol), and 1, Phosphorous tribromide (0.37 mL, 4 mmol) was added dropwise to a mixture of 2-dimethoxyethane (10 mL) at 0 ° C., and the mixture was stirred at room temperature for 2 hours and then at 50 ° C. for 30 minutes. The reaction mixture was cooled to 0 ° C., water was added, the mixture was extracted with ethyl acetate, and the organic layer was washed with saturated brine. After drying over anhydrous magnesium sulfate and filtering, the solvent was evaporated under reduced pressure, and the residue was purified by silica gel column chromatography to obtain the title compound (0.14 g).
1 H-NMR Spectrum (CDCl3) δ (ppm): 1.33 (18H, s), 4.45 (2H, s), 6.63 (1H, s), 7.43 (1H, dd, J = 4.8, 8.0 Hz), 8.28 ( 1H, dd, J = 2.0,8.0Hz), 8.61 (1H, dd, J = 2.0,4.8Hz).
［参考例２６］２－［（４－ブロモベンジル）オキシ］ピリジンの合成
Figure JPOXMLDOC01-appb-C000074
 　窒素雰囲気下、４－ブロモベンジルアルコール（１８ｇ，９４．３ｍｍｏｌ）のジメチルスルホキシド（８５ｍＬ）溶液に、室温下でカリウムｔｅｒｔ－ブトキシド（１１．５ｇ，９９ｍｍｏｌ）を少しずつ加え１０分間撹拌した。この溶液に、水浴冷却下、２－フルオロピリジン（１２．３ｇ，１２３ｍｍｏｌ）を３０分間で滴下した。室温で２時間撹拌した後、酢酸エチルと５％食塩水を加え抽出した。有機層を水、５％食塩水で順次洗浄した後、減圧下溶媒を留去し、標記化合物（２４．３ｇ）を黄色油状物として得た。
1H－NMR  Spectrum  (CDCl3)δ(ppm)：5.33(2H,s),  6.87－6.70(1H,m),  6.98－7.02(1H,m),  7.38－7.44(2H,m),  7.55－7.60(2H,m),  7.71－7.76(1H,m),  8.15－8.18(1H,m). [Reference Example 26] Synthesis of 2-[(4-bromobenzyl) oxy] pyridine
Figure JPOXMLDOC01-appb-C000074
 Under a nitrogen atmosphere, potassium tert-butoxide (11.5 g, 99 mmol) was added little by little to a solution of 4-bromobenzyl alcohol (18 g, 94.3 mmol) in dimethyl sulfoxide (85 mL) at room temperature, and the mixture was stirred for 10 minutes. To this solution, 2-fluoropyridine (12.3 g, 123 mmol) was added dropwise over 30 minutes while cooling in a water bath. After stirring at room temperature for 2 hours, ethyl acetate and 5% brine were added for extraction. The organic layer was washed successively with water and 5% brine, and the solvent was evaporated under reduced pressure to give the title compound (24.3 g) as a yellow oil.
1 H-NMR Spectrum (CDCl 3 ) δ (ppm): 5.33 (2H, s), 6.87-6.70 (1H, m), 6.98-7.02 (1H, m), 7.38-7.44 (2H, m), 7.55- 7.60 (2H, m), 7.71-7.76 (1H, m), 8.15-8.18 (1H, m).
　参考例２７は、参考例２６の別途合成法である。 Reference Example 27 is a separate synthesis method of Reference Example 26.
［参考例２７］２－［（４－ブロモベンジル）オキシ］ピリジンの合成
Figure JPOXMLDOC01-appb-C000075
 　窒素雰囲気下、４－ブロモベンジルアルコール（６００ｇ，３．２１ｍｏｌ）と２－フルオロピリジン（３４３ｇ，３．５３ｍｏｌ）のテトラヒドロフラン（１０６９ｍＬ）溶液に、７℃冷却下、カリウムｔｅｒｔ－ブトキシド（３９６ｇ，３．５３ｍｏｌ）のテトラヒドロフラン（３２０８ｍＬ）溶液を滴下した（６３ｍｉｎ，９．２～２０．５℃）。２２℃で３時間撹拌した後、５％炭酸水素ナトリウム水溶液（炭酸水素ナトリウム：１６０ｇと水：３２０８ｍＬから調製）を滴下した（２０ｍｉｎ，２１．０～２３．９℃）。次いでヘプタン（３２２０ｍＬ）を加え抽出、有機層を水（８００ｍＬ）で洗浄した。減圧濃縮（約３２００ｍＬまで）、エタノール（１６０４ｍＬ）を加え、減圧濃縮した（約３２００ｍＬまで）。次いで、ヘプタン（３２００ｍＬ）を加え減圧濃縮、さらにヘプタン（３２００ｍＬ）を加え減圧濃縮し、標記化合物のヘプタン溶液（目的物を７８９ｇ含有，２６０３ｇ）を褐色油状物として得た（収率：９３．２％）。
1H－NMR  Spectrum  (CDCl3)δ(ppm)：5.33(2H,s),  6.87－6.70(1H,m),  6.98－7.02(1H,m),  7.38－7.44(2H,m),  7.55－7.60(2H,m),  7.71－7.76(1H,m),  8.15－8.18(1H,m). [Reference Example 27] Synthesis of 2-[(4-bromobenzyl) oxy] pyridine
Figure JPOXMLDOC01-appb-C000075
 In a nitrogen atmosphere, potassium tert-butoxide (396 g, 3.21 mol) in a tetrahydrofuran (1069 mL) solution of 4-bromobenzyl alcohol (600 g, 3.21 mol) and 2-fluoropyridine (343 g, 3.53 mol) was cooled at 7 ° C. 53 mol) in tetrahydrofuran (3208 mL) was added dropwise (63 min, 9.2 to 20.5 ° C.). After stirring at 22 ° C. for 3 hours, 5% aqueous sodium hydrogen carbonate solution (prepared from 160 g of sodium bicarbonate and water: 3208 mL) was added dropwise (20 min, 21.0 to 23.9 ° C.). Subsequently, heptane (3220 mL) was added and extracted, and the organic layer was washed with water (800 mL). Concentrated under reduced pressure (to about 3200 mL), ethanol (1604 mL) was added, and concentrated under reduced pressure (to about 3200 mL). Subsequently, heptane (3200 mL) was added and the mixture was concentrated under reduced pressure. Further heptane (3200 mL) was added and the mixture was concentrated under reduced pressure to obtain a heptane solution of the title compound (containing 789 g of the desired product, 2603 g) as a brown oil (yield: 93.2). %).
1 H-NMR Spectrum (CDCl 3 ) δ (ppm): 5.33 (2H, s), 6.87-6.70 (1H, m), 6.98-7.02 (1H, m), 7.38-7.44 (2H, m), 7.55- 7.60 (2H, m), 7.71-7.76 (1H, m), 8.15-8.18 (1H, m).
［参考例２８］｛４－［（ピリジン－２－イロキシ）メチル］フェニル｝ボロン酸の合成
Figure JPOXMLDOC01-appb-C000076
 　窒素雰囲気下、２－［（４－ブロモベンジル）オキシ］ピリジン（５０ｇ，１９０ｍｍｏｌ）のテトラヒドロフラン（２００ｍＬ）溶液を－７８℃に冷却し、２．６Ｍのｎ－ブチルリチウムヘキサン溶液（８８ｍＬ，２２８ｍｍｏｌ）を滴下した。４５分間撹拌した後、同温でトリメトキシボラン（２９．６ｇ，２８５ｍｍｏｌ）を滴下した。３０分後、飽和塩化アンモニウム水溶液と水を加えてクエンチし、酢酸エチルで抽出した。有機層を飽和塩化アンモニウム水溶液と飽和食塩水の混合液で洗浄し、無水硫酸ナトリウムで乾燥した後、減圧下溶媒を留去した。残渣へアセトニトリル（２００ｍＬ）を加え、７０℃で３０分間懸濁撹拌した後、冷却し、４℃で終夜撹拌した。析出している固体を濾過し、標記化合物（１１．２ｇ）を白色固体として得た。
1H－NMR  Spectrum  (CDCl3)δ(ppm)：4.62(2H,s),  5.42(2H,s),　6.83(1H,d,J＝8.4Hz),  6.87－6.92(1H,m),  7.50(2H,d,J＝8.0Hz),  7.57－7.62(1H,m),  7.75(2H,d,J＝8.0Hz),  8.16－8.19(1H,m). [Reference Example 28] Synthesis of {4-[(pyridine-2-yloxy) methyl] phenyl} boronic acid
Figure JPOXMLDOC01-appb-C000076
 Under a nitrogen atmosphere, a tetrahydrofuran (200 mL) solution of 2-[(4-bromobenzyl) oxy] pyridine (50 g, 190 mmol) was cooled to −78 ° C., and a 2.6 M n-butyllithium hexane solution (88 mL, 228 mmol) was obtained. Was dripped. After stirring for 45 minutes, trimethoxyborane (29.6 g, 285 mmol) was added dropwise at the same temperature. After 30 minutes, the reaction was quenched by adding saturated aqueous ammonium chloride and water, and extracted with ethyl acetate. The organic layer was washed with a mixed solution of saturated aqueous ammonium chloride and saturated brine, dried over anhydrous sodium sulfate, and the solvent was evaporated under reduced pressure. Acetonitrile (200 mL) was added to the residue, suspended and stirred at 70 ° C. for 30 minutes, cooled, and stirred at 4 ° C. overnight. The precipitated solid was filtered to obtain the title compound (11.2 g) as a white solid.
1 H-NMR Spectrum (CDCl 3 ) δ (ppm): 4.62 (2H, s), 5.42 (2H, s), 6.83 (1H, d, J = 8.4 Hz), 6.87-6.92 (1H, m), 7.50 (2H, d, J = 8.0Hz), 7.57-7.62 (1H, m), 7.75 (2H, d, J = 8.0Hz), 8.16-8.19 (1H, m).
［参考例２９］２－｛［４－（５，５－ジメチル－１，３，２－ジオキサボリナン－２－イル）ベンジル］オキシ｝ピリジンの合成
Figure JPOXMLDOC01-appb-C000077
 　２－［（４－ブロモベンジル）オキシ］ピリジン（７８９ｇ，２．９９ｍｏｌ）のヘプタン溶液（２６０３ｇ）にヘプタン（９３９ｍＬ）、テトラヒドロフラン（１１９９ｍＬ）を加え、窒素雰囲気下、撹拌しながらドライアイス／エタノールバスでゆっくりと冷却した。４５分後、冷却を中断、内温：－１２℃で２－［（４－ブロモベンジル）オキシ］ピリジン（０．９ｇ）を加えた。冷却を再開、－２０℃／ｈで冷却した。約３時間後、１．６６Ｍ　ｎ－ブチルリチウムヘキサン溶液（１９８０ｍＬ，３．２９ｍｏｌ）を滴下した（８０ｍｉｎ，－６７．０～６１．４℃）。０．５時間撹拌した後、同温でトリイソプロポキシボラン（６７４ｇ，３．５６ｍｏｌ）を滴下した（１３４ｍｉｎ，－６８．２～６０．３℃）。同温で０．５時間撹拌後、氷水バス冷却に換え、終夜撹拌した（外温：０℃）。翌日、水（５６００ｍＬ）を滴下、分液装置に移して水層へ抽出した（ｐＨ：１１．２）。酢酸エチル（４８００ｍＬ）を加え、撹拌しながら濃塩酸（約２８０ｍＬ）を滴下し（内温２０℃以下）、ｐＨ：７．１に調整した。有機層を分液、５％食塩水（約９００ｇ）で洗浄後、減圧下濃縮した。残渣にイソプロピルアルコール（３３００ｍＬ）を加え減圧濃縮、さらにイソプロピルアルコール（３３００ｍＬ）を加え減圧濃縮し、｛４－［（ピリジン－２－イロキシ）メチル］フェニル｝ボロン酸（６４６ｇ）のイソプロピルアルコール溶液（２６７１ｇ）を得た（収率：９４．４％）。得られた溶液を６０℃に加熱し、２，２－ジメチル－１，３－プロパンジオール（３５４ｇ，３．４１ｍｏｌ）の入った容器へ吸引濾過して不溶物を除去ながら加え、次いでイソプロピルアルコール（６８５ｍＬ）で洗い込んだ。溶解確認後、バス温度：２０℃で撹拌、内温：２８．８℃で結晶析出を確認した。１．５時間後、バス温度：－２０℃とし、終夜撹拌した。析出した結晶を濾過、０℃に冷却した少量のイソプロピルアルコールで結晶を洗浄した。減圧乾燥し、標記化合物（７７９ｇ）を白色結晶として得た（収率：９２．２％）。
1H－NMR  Spectrum  (DMSO－d6)δ(ppm)：0.94(6H,s),  3.74(4H,s),　5.35(2H,s),  6.87(1H,d,J＝8.4Hz),  6.96－7.00(1H,m),  7.39(2H,d,J＝8.0Hz),  7.67－7.74(3H,m),  8.14－8.17(1H,m). [Reference Example 29] Synthesis of 2-{[4- (5,5-dimethyl-1,3,2-dioxaborin-2-yl) benzyl] oxy} pyridine
Figure JPOXMLDOC01-appb-C000077
 Heptane (939 mL) and tetrahydrofuran (1199 mL) were added to a heptane solution (2603 g) of 2-[(4-bromobenzyl) oxy] pyridine (789 g, 2.99 mol), and a dry ice / ethanol bath was stirred under a nitrogen atmosphere. Cool slowly. After 45 minutes, cooling was discontinued and 2-[(4-bromobenzyl) oxy] pyridine (0.9 g) was added at an internal temperature of -12 ° C. Cooling was resumed and it was cooled at −20 ° C./h. After about 3 hours, a 1.66M n-butyllithium hexane solution (1980 mL, 3.29 mol) was added dropwise (80 min, −67.0 to 61.4 ° C.). After stirring for 0.5 hour, triisopropoxyborane (674 g, 3.56 mol) was added dropwise at the same temperature (134 min, −68.2 to 60.3 ° C.). After stirring at the same temperature for 0.5 hour, it was changed to ice water bath cooling and stirred overnight (outside temperature: 0 ° C.). The next day, water (5600 mL) was added dropwise, transferred to a separator and extracted into the aqueous layer (pH: 11.2). Ethyl acetate (4800 mL) was added, and concentrated hydrochloric acid (about 280 mL) was added dropwise with stirring (internal temperature 20 ° C. or lower) to adjust the pH to 7.1. The organic layer was separated, washed with 5% brine (about 900 g), and concentrated under reduced pressure. Isopropyl alcohol (3300 mL) was added to the residue and concentrated under reduced pressure. Isopropyl alcohol (3300 mL) was further added and concentrated under reduced pressure, and a solution of {4-[(pyridin-2-yloxy) methyl] phenyl} boronic acid (646 g) in isopropyl alcohol (2671 g). (Yield: 94.4%). The obtained solution was heated to 60 ° C., added to a container containing 2,2-dimethyl-1,3-propanediol (354 g, 3.41 mol) by suction filtration while removing insoluble matters, and then isopropyl alcohol ( 685 mL). After confirmation of dissolution, stirring was performed at a bath temperature of 20 ° C., and crystal precipitation was confirmed at an internal temperature of 28.8 ° C. After 1.5 hours, the bath temperature was set to −20 ° C. and stirred overnight. The precipitated crystals were filtered and washed with a small amount of isopropyl alcohol cooled to 0 ° C. Drying under reduced pressure gave the title compound (779 g) as white crystals (yield: 92.2%).
1 H-NMR Spectrum (DMSO-d 6 ) δ (ppm): 0.94 (6H, s), 3.74 (4H, s), 5.35 (2H, s), 6.87 (1H, d, J = 8.4 Hz), 6.96 -7.00 (1H, m), 7.39 (2H, d, J = 8.0Hz), 7.67-7.74 (3H, m), 8.14-8.17 (1H, m).
［参考例３０］ジ－ｔｅｒｔ－ブチル　［３－（３－｛４［（ピリジン－２－イロキシ）メチル］ベンジル｝イソキサゾール－５－イル）ピリジン－２－イル］イミドジカーボネートの合成
Figure JPOXMLDOC01-appb-C000078
 　窒素雰囲気下、ジ－ｔｅｒｔ－ブチル　｛３－［３－（クロロメチル）イソキサゾール－５－イル］ピリジン－２－イル｝イミドジカーボネート（１６４ｍｇ，０．４０ｍｍｏｌ）、｛４－［（ピリジン－２－イロキシ）メチル］フェニル｝ボロン酸（１３８ｍｇ，０．６０ｍｍｏｌ）、炭酸セシウム（３９１ｍｇ，１．２０ｍｍｏｌ）、ヨウ化銅（Ｉ）（３．９ｍｇ，５ｍｏｌ％）、および１，２－ジメトキシエタン（２．０ｍＬ）の混合物に［１，１’－ビス（ジフェニルフォスフィノ）フェロセン］ジクロロパラジウム（ＩＩ）・ジクロロメタンコンプレックス（１６．４ｍｇ，５ｍｏｌ％）を加え、８０℃で１．５時間撹拌した。｛４－［（ピリジン－２－イロキシ）メチル］フェニル｝ボロン酸（４６ｍｇ，０．２０ｍｍｏｌ）を追加し、さらに４．５時間撹拌した。冷却後、酢酸エチルと５％食塩水を加え、不溶物を濾過した後、濾液を分液ロートへ移し分層した。有機層を５％食塩水で洗浄し、無水硫酸マグネシウムで乾燥した後、減圧下溶媒を留去した。残渣をシリカゲルカラムクロマトグラフィーで精製し、標記化合物（１７３ｍｇ）を淡黄色油状物として得た。
1H－NMR  Spectrum  (CDCl3)δ(ppm)：1.23(9H,s),  4.05(2H,s),　5.34(2H,s),  6.32(1H,s),　6.76－6.79(1H,m),  6.86－6.90(1H,m),  7.28(2H,d,  J＝8.0Hz),  7.38－7.43(3H,m),  7.55－7.60(1H,m),  8.15－8.18(1H,m),  8.27(1H,dd,J＝2.0,8.0Hz),  8.57(1H,dd,J＝2.0,7.6Hz). [Reference Example 30] Synthesis of di-tert-butyl [3- (3- {4 [(pyridin-2-yloxy) methyl] benzyl} isoxazol-5-yl) pyridin-2-yl] imide dicarbonate
Figure JPOXMLDOC01-appb-C000078
 Under a nitrogen atmosphere, di-tert-butyl {3- [3- (chloromethyl) isoxazol-5-yl] pyridin-2-yl} imide dicarbonate (164 mg, 0.40 mmol), {4-[(pyridine-2 -Iroxy) methyl] phenyl} boronic acid (138 mg, 0.60 mmol), cesium carbonate (391 mg, 1.20 mmol), copper (I) iodide (3.9 mg, 5 mol%), and 1,2-dimethoxyethane ( 2.0 mL), [1,1′-bis (diphenylphosphino) ferrocene] dichloropalladium (II) · dichloromethane complex (16.4 mg, 5 mol%) was added, and the mixture was stirred at 80 ° C. for 1.5 hours. {4-[(Pyridin-2-yloxy) methyl] phenyl} boronic acid (46 mg, 0.20 mmol) was added, and the mixture was further stirred for 4.5 hours. After cooling, ethyl acetate and 5% brine were added, insolubles were filtered, and the filtrate was transferred to a separatory funnel and separated. The organic layer was washed with 5% brine and dried over anhydrous magnesium sulfate, and then the solvent was distilled off under reduced pressure. The residue was purified by silica gel column chromatography to obtain the title compound (173 mg) as a pale yellow oil.
1 H-NMR Spectrum (CDCl 3 ) δ (ppm): 1.23 (9H, s), 4.05 (2H, s), 5.34 (2H, s), 6.32 (1H, s), 6.76-6.79 (1H, m) , 6.86-6.90 (1H, m), 7.28 (2H, d, J = 8.0Hz), 7.38-7.43 (3H, m), 7.55-7.60 (1H, m), 8.15-8.18 (1H, m), 8.27 (1H, dd, J = 2.0,8.0Hz), 8.57 (1H, dd, J = 2.0,7.6Hz).
［参考例３１］３－（３－（４－ピリジン－２－イルオキシメチル）－ベンジル）－イソキサゾール－５－イル）－ピリジン－２－イルアミン
Figure JPOXMLDOC01-appb-C000079
 　ジ－ｔｅｒｔ－ブチル　［３－（３－｛４［（ピリジン－２－イロキシ）メチル］ベンジル｝イソキサゾール－５－イル）ピリジン－２－イル］イミドジカーボネート（２８．８ｍｇ，５１．６μｍｏｌ）をアセトニトリル（０．６ｍＬ）に溶解し、氷水冷下、濃塩酸（６０μＬ，６９０μｍｏｌ）を滴下し同温で１時間撹拌した。さらに濃塩酸（１４０μＬ，１．６１ｍｍｏｌ）を滴下し、同温で１時間、２０℃で３．５時間撹拌した。氷水冷下、反応液に０．５Ｎ水酸化ナトリウム水溶液と酢酸エチルを加え、抽出した。有機層を５％食塩水で洗浄し、無水硫酸マグネシウムで乾燥した後、減圧下溶媒を留去し、標記化合物（１８．３ｍｇ）を淡黄色油状物として得た。
1H－NMR  Spectrum  (CDCl3)δ(ppm)：4.07(2H,s),  5.37(2H,s),　5.42(2H,brs),  6.25(1H,s),　6.71(1H,dd,J＝5.2,7.6Hz),  6.80(1H,d,J＝8.4Hz),  6.87－6.91(1H,m),  7.30(2H,d,J＝7.6Hz),  7.44(2H,d,J＝7.6Hz),  7.56－7.61(1H,m),  7.70(1H,dd,J＝2.0,7.6Hz),  8.14(1H,dd,J＝2.0,4.8Hz),  8.16－8.19(1H,m). [Reference Example 31] 3- (3- (4-Pyridin-2-yloxymethyl) -benzyl) -isoxazol-5-yl) -pyridin-2-ylamine
Figure JPOXMLDOC01-appb-C000079
 Di-tert-butyl [3- (3- {4 [(pyridin-2-yloxy) methyl] benzyl} isoxazol-5-yl) pyridin-2-yl] imide dicarbonate (28.8 mg, 51.6 μmol) It melt | dissolved in acetonitrile (0.6 mL), Concentrated hydrochloric acid (60 microliters, 690 micromol) was dripped under ice-water cooling, and it stirred at the same temperature for 1 hour. Further, concentrated hydrochloric acid (140 μL, 1.61 mmol) was added dropwise, and the mixture was stirred at the same temperature for 1 hour and at 20 ° C. for 3.5 hours. Under cooling with ice water, 0.5N aqueous sodium hydroxide solution and ethyl acetate were added to the reaction solution and extracted. The organic layer was washed with 5% brine and dried over anhydrous magnesium sulfate, and the solvent was evaporated under reduced pressure to give the title compound (18.3 mg) as a pale yellow oil.
1 H-NMR Spectrum (CDCl 3 ) δ (ppm): 4.07 (2H, s), 5.37 (2H, s), 5.42 (2H, brs), 6.25 (1H, s), 6.71 (1H, dd, J = 5.2,7.6Hz), 6.80 (1H, d, J = 8.4Hz), 6.87-6.91 (1H, m), 7.30 (2H, d, J = 7.6Hz), 7.44 (2H, d, J = 7.6Hz) 7.56-7.61 (1H, m), 7.70 (1H, dd, J = 2.0,7.6Hz), 8.14 (1H, dd, J = 2.0,4.8Hz), 8.16-8.19 (1H, m).
　参考例３２～３３は、参考例３０～３１の別途合成法である。 Reference examples 32 to 33 are separate synthesis methods of reference examples 30 to 31.
［参考例３２］ジ－ｔｅｒｔ－ブチル　［３－（３－｛４［（ピリジン－２－イロキシ）メチル］ベンジル｝イソキサゾール－５－イル）ピリジン－２－イル］イミドジカーボネートの合成
Figure JPOXMLDOC01-appb-C000080
 　１バッチ目は以下のように実施した。あらかじめ窒素置換した５００Ｌ反応缶２に、窒素気流下、ジ－ｔｅｒｔ－ブチル　｛３－［３－（クロロメチル）イソキサゾール－５－イル］ピリジン－２－イル｝イミドジカーボネート（２０．８０ｋｇ，５０．７５ｍｏｌ）、２－｛［４－（５，５－ジメチル－１，３，２－ジオキサボリナン－２－イル）ベンジル］オキシ｝ピリジン（１９．６１ｋｇ，６６．００ｍｏｌ，１．３０Ｍ／Ｍ）、（オキシジ－２，１－フェニレン）ビス（ジフェニルホスフィン）（１．３６７ｋｇ，２．５４ｍｏｌ，０．０５Ｍ／Ｍ）、炭酸カリウム（９．１１ｋｇ，６５．９１ｍｏｌ，１．３０Ｍ／Ｍ）を加えた後、缶内を再度窒素置換してＮ，Ｎ－ジメチルホルムアミド（１４７ｋｇ，７．０８ｗ／ｗ）を加え、攪拌を開始した。次いで、内温１５～２５℃で、－０．０９０ＭＰａ以上の減圧度で３～５分維持してから、窒素で減圧を解除した。この操作を合計５回繰り返して溶液の脱気をした。脱気終了後、酢酸パラジウムのＮ，Ｎ－ジメチルホルムアミド溶液（酢酸パラジウム（０．５７０ｋｇ，２．５４ｍｏｌ，０．０５Ｍ／Ｍ）と脱気済のＮ，Ｎ－ジメチルホルムアミド（９．８ｋｇ，０．５ｗ／ｗ，洗い込み用に一部取分け）の混液）を加え、取分けた脱気済のＮ，Ｎ－ジメチルホルムアミドで洗い込んだ。続けて、１０分攪拌した後、直ちに内温２０～３０℃で脱気済の水（１０．４ｋｇ，０．５ｗ／ｗ）を滴下し、減圧度－０．０８７ＭＰａまで減圧して窒素で減圧を解除する操作を３回繰り返した。その後、速やかに約６０℃の温水を循環して内温を５５～６５℃に調節し、加熱開始から３時間攪拌した。ＨＰＬＣ分析により反応の終了を確認した後、内温０～２５℃でトルエン（９０ｋｇ，４．３４ｗ／ｗ）を加え、同温度範囲で水（１５６ｋｇ，７．５ｗ／ｗ）を滴下した。続いて、内温１５～３０℃で３０分攪拌後、３０分以上静置して下層を除去した。缶内の上層に水（１０４ｋｇ，５．０ｗ／ｗ）を加え、内温１５～３０℃で５分攪拌後、終夜静置し、不溶物を含まない下層のみを除去した。上層と不溶物を含む下層をセライト５０３ＲＶ（２．８ｋｇ，０．１３５ｗ／ｗ）を敷いたろ過器で加圧ろ過し、トルエン（１８．０ｋｇ，０．８６７ｗ／ｗ，送り出し、洗い込み用に一部取分けた）で缶及びろ過器をかけ洗った。得られたろ液及び洗液を５００Ｌ反応缶２に戻し、先に取分けたトルエンで洗い込んだ。その後、内温を１５～３０℃に調整した後、３０分以上静置して下層を除去した。攪拌速度をほぼ最大に調節し、内温１５～３０℃でｎ－ヘプタン（１５２ｋｇ，７．３２ｗ／ｗ）を１時間以上かけて滴下した後、同温度範囲で２時間以上攪拌した。続いて、内温１５～３０℃でチオシアヌル酸（０．９０ｋｇ，５．０８ｍｏｌ，０．１Ｍ／Ｍ）を３０分以上かけて分割投入後、同温度範囲で１時間以上攪拌した。再び内温１５～３０℃でチオシアヌル酸（０．９０ｋｇ，５．０８ｍｏｌ，０．１Ｍ／Ｍ）を３０分以上かけて分割投入し、同温度範囲で終夜攪拌した。終夜攪拌後、缶の内容液を、あらかじめ準備しておいたろ過器で活性炭ろ過し、ｎ－ヘプタン－トルエン混液（ｎ－ヘプタン（１３０ｋｇ）とトルエン（８３ｋｇ）の混液，一部活性炭（精製白鷺）の湿潤用に取分け）で缶及びろ過器を洗い込んだ。再度、チオシアヌル酸（１．８０ｋｇ，１０．１６ｍｏｌ，０．２Ｍ／Ｍ）を投入した後、同量のセライト５０３ＲＶ、活性炭（精製白鷺）及びｎ－ヘプタン－トルエン混液を用いて活性炭ろ過処理を行なった。そこで，得られたろ液及び洗液を５００Ｌ反応缶１に加え、４０～７０℃の温水循環下、内容液が目視で約１００Ｌとなるまで減圧濃縮した。なお、濃縮残渣は２バッチ目の活性炭ろ過が終了するまで、窒素雰囲気下、内温３０℃以下で静置した。
　２バッチ目として上記と同様の操作を実施した。２バッチ目のろ液及び洗液を５００Ｌ反応缶１に加え、１バッチ目の濃縮残渣と合わせて減圧濃縮を開始した。６０～７０℃の温水循環下、留出が弱まったところで、トルエン（１４４ｋｇ）を添加した後、再度、６０～７０℃の温水循環下、留出が弱まるまで減圧濃縮した。ここで、濃縮残渣を分析し、濃縮残渣中のジ－ｔｅｒｔ－ブチル　［３－（３－｛４［（ピリジン－２－イロキシ）メチル］ベンジル｝イソキサゾール－５－イル）ピリジン－２－イル］イミドジカーボネート含量及びトルエン含量からトルエン／目的物の比率（０．１６７ｗ／ｗ）を算出した。トルエン（２９．６６ｋｇ，トルエン／目的物の比率０．７００ｗ／ｗ相当）を添加し、内温１５～３０℃で３０分以上攪拌して、標記化合物のトルエン溶液（目的物を４２．３７ｋｇ含有，収率：７４．７％）を得た。
ＨＰＬＣ条件　カラム：CAPCELL  PAK　C18  MGII  (5μm,　150x4.6mmI.D.,　SHISEIDO)，移動相：アセトニトリル／水／トリフルオロ酢酸=１８０／８２０／１～９００／１００／１（ｖ／ｖ／ｖ）。 [Reference Example 32] Synthesis of di-tert-butyl [3- (3- {4 [(pyridin-2-yloxy) methyl] benzyl} isoxazol-5-yl) pyridin-2-yl] imide dicarbonate
Figure JPOXMLDOC01-appb-C000080
 The first batch was carried out as follows. Into a 500-liter reactor 2 previously purged with nitrogen, di-tert-butyl {3- [3- (chloromethyl) isoxazol-5-yl] pyridin-2-yl} imide dicarbonate (20.80 kg, 50 .75 mol), 2-{[4- (5,5-dimethyl-1,3,2-dioxaborin-2-yl) benzyl] oxy} pyridine (19.61 kg, 66.00 mol, 1.30 M / M), (Oxydi-2,1-phenylene) bis (diphenylphosphine) (1.367 kg, 2.54 mol, 0.05 M / M) and potassium carbonate (9.11 kg, 65.91 mol, 1.30 M / M) were added. Thereafter, the inside of the can was again purged with nitrogen, N, N-dimethylformamide (147 kg, 7.08 w / w) was added, and stirring was started. Subsequently, the internal temperature was maintained at 15 to 25 ° C. and a reduced pressure of −0.090 MPa or more for 3 to 5 minutes, and then the reduced pressure was released with nitrogen. This operation was repeated a total of 5 times to degas the solution. After completion of degassing, a solution of palladium acetate in N, N-dimethylformamide (palladium acetate (0.570 kg, 2.54 mol, 0.05 M / M) and degassed N, N-dimethylformamide (9.8 kg, 0 5 w / w, partially mixed for washing) was added, and washed with the separated degassed N, N-dimethylformamide. After stirring for 10 minutes, degassed water (10.4 kg, 0.5 w / w) was immediately added dropwise at an internal temperature of 20-30 ° C., the pressure was reduced to -0.087 MPa, and the pressure was reduced with nitrogen. The operation of releasing was repeated three times. Thereafter, warm water at about 60 ° C. was quickly circulated to adjust the internal temperature to 55 to 65 ° C., and the mixture was stirred for 3 hours from the start of heating. After confirming the completion of the reaction by HPLC analysis, toluene (90 kg, 4.34 w / w) was added at an internal temperature of 0 to 25 ° C., and water (156 kg, 7.5 w / w) was added dropwise within the same temperature range. Subsequently, the mixture was stirred at an internal temperature of 15 to 30 ° C. for 30 minutes and then allowed to stand for 30 minutes or more to remove the lower layer. Water (104 kg, 5.0 w / w) was added to the upper layer in the can, and the mixture was stirred at an internal temperature of 15 to 30 ° C. for 5 minutes and then allowed to stand overnight to remove only the lower layer containing no insoluble matter. The upper layer and the lower layer containing insolubles are pressure filtered with a filter laid with Celite 503RV (2.8 kg, 0.135 w / w), and toluene (18.0 kg, 0.867 w / w, sent out for washing) In some cases, it was washed with a can and a filter. The obtained filtrate and washings were returned to the 500 L reactor 2 and washed with the previously separated toluene. Thereafter, the internal temperature was adjusted to 15 to 30 ° C., and then allowed to stand for 30 minutes or more to remove the lower layer. The stirring speed was adjusted to the maximum, n-heptane (152 kg, 7.32 w / w) was added dropwise at an internal temperature of 15 to 30 ° C. over 1 hour, and then stirred at the same temperature range for 2 hours or more. Subsequently, thiocyanuric acid (0.90 kg, 5.08 mol, 0.1 M / M) was added in portions over 30 minutes at an internal temperature of 15 to 30 ° C., and then stirred for 1 hour or more in the same temperature range. Again, thiocyanuric acid (0.90 kg, 5.08 mol, 0.1 M / M) was added in portions over 30 minutes at an internal temperature of 15 to 30 ° C. and stirred overnight in the same temperature range. After stirring overnight, the contents of the can were filtered with activated carbon using a pre-prepared filter, and mixed with n-heptane-toluene (n-heptane (130 kg) and toluene (83 kg), partially activated carbon (purified white rice cake). The cans and the filter were washed in (2). After thiocyanuric acid (1.80 kg, 10.16 mol, 0.2 M / M) was added again, activated carbon filtration was performed using the same amount of Celite 503RV, activated carbon (purified birch) and n-heptane-toluene mixture. It was. Therefore, the obtained filtrate and washing solution were added to a 500 L reactor 1 and concentrated under reduced pressure while circulating hot water at 40 to 70 ° C. until the content solution was visually about 100 L. The concentrated residue was allowed to stand at an internal temperature of 30 ° C. or lower in a nitrogen atmosphere until the second batch of activated carbon filtration was completed.
The same operation as described above was performed as the second batch. The filtrate and washing solution of the second batch were added to the 500 L reactor 1 and the vacuum concentration was started together with the concentrated residue of the first batch. Toluene (144 kg) was added when the distillation was weakened under hot water circulation at 60 to 70 ° C., and then concentrated again under reduced pressure under hot water circulation at 60 to 70 ° C. until distillation was weakened. Here, the concentrated residue was analyzed, and di-tert-butyl [3- (3- {4 [(pyridin-2-yloxy) methyl] benzyl} isoxazol-5-yl) pyridin-2-yl] in the concentrated residue was analyzed. The ratio of toluene / target product (0.167 w / w) was calculated from the imide dicarbonate content and the toluene content. Toluene (29.66 kg, corresponding to a toluene / target product ratio of 0.700 w / w) was added, and the mixture was stirred at an internal temperature of 15 to 30 ° C. for 30 minutes or longer to give a toluene solution of the title compound (containing 42.37 kg of the target product). Yield: 74.7%).
HPLC conditions Column: CAPCELL PAK C18 MGII (5 μm, 150 × 4.6 mm I.D., SHISEIDO), mobile phase: acetonitrile / water / trifluoroacetic acid = 180/820/1 to 900/100/1 (v / v / v) .
［参考例３３］３－（３－（４－ピリジン－２－イルオキシメチル）－ベンジル）－イソキサゾール－５－イル）－ピリジン－２－イルアミン
Figure JPOXMLDOC01-appb-C000081
 　ジ－ｔｅｒｔ－ブチル　［３－（３－｛４［（ピリジン－２－イロキシ）メチル］ベンジル｝イソキサゾール－５－イル）ピリジン－２－イル］イミドジカーボネートのトルエン溶液（４２．３７ｋｇ（７５．８５　ｍｏｌ）含有）に内温－５～２０℃でギ酸（１８１ｋｇ，４．２７ｗ／ｗ）を滴下し、内温を２２～３２℃で１９～２０時間攪拌した。ＨＰＬＣ分析で反応終了を確認した後、内温－５～１０℃まで冷却し、内容液を２分割して５００Ｌ反応缶１及び２にそれぞれ加えた。
　５００Ｌ反応缶１について、以下のように後処理を実施した。攪拌下、内温－５～２０℃で水（７４ｋｇ，１．７５ｗ／ｗ）を滴下し、さらに内温０～２５℃でｔｅｒｔ－ブチルメチルエーテル（３１．４ｋｇ，０．７４ｗ／ｗ）とｎ－ヘプタン（２９．０ｋｇ，０．６８４ｗ／ｗ）を加えた。内温１５～２５℃で５分攪拌し、３０分以上静置して下層を分取した。下層を缶に戻し、再び内温０～２５℃でｔｅｒｔ－ブチルメチルエーテル（３１．４ｋｇ，０．７４ｗ／ｗ）とｎ－ヘプタン（２９．０ｋｇ，０．６８４ｗ／ｗ）を加え、内温１５～２５℃で５分攪拌後、３０分以上静置して、もう一度下層を分取した。下層を缶に戻し、まず、内温０～２５℃で４８％水酸化ナトリウム水溶液（１１６ｋｇ，水酸化ナトリウムとして１３９２．０ｍｏｌ，１８．３５Ｍ／Ｍ）を滴下した。次に、同温度範囲で酢酸エチル（９６ｋｇ，２．２６ｗ／ｗ）を加え、４８％水酸化ナトリウム水溶液（２０．５ｋｇ，水酸化ナトリウムとして２４６．０ｍｏｌ，３．２４Ｍ／Ｍ）を滴下した。さらに、ここに同温度範囲で約８％水酸化ナトリウム水溶液（４８％水酸化ナトリウム水溶液（１２．７ｋｇ，水酸化ナトリウムとして１５２．４ｍｏｌ，２．００Ｍ／Ｍ）と水（６４ｋｇ，１．５ｗ／ｗ）の混液）を下層のｐＨがｐＨ８．００～９．００，実測値：ｐＨ８．５８）となるまで滴下した（０．７５ｋｇ使用）。その後、内温２０～３０℃で１時間以上攪拌してから終夜静置後、下層のｐＨを再確認（実測値ｐＨ８．２９）し、下層を除去した。缶に残った上層に約５％炭酸水素ナトリウム水溶液（炭酸水素ナトリウム（５．３ｋｇ，６３．０９ｍｏｌ）と水（１０１ｋｇ，２．３７５ｗ／ｗ）の混液）を加え、内温２０～３０℃で１時間以上攪拌後、３０分以上静置した。下層（ｐＨ８．６０）を除去した後、上層に水（１０６ｋｇ，２．５ｗ／ｗ）を加え、内温２０～３０℃で１時間以上攪拌後、３０分以上静置して、再び下層（ｐＨ７．１７）を除去した。
　５００Ｌ反応缶２について、５００Ｌ反応缶１と並行して同様の後処理を実施した。
　５００Ｌ反応缶１の内容液を５００Ｌ反応缶２に移送し、５５～６５℃の温水循環下、内容液が約１００Ｌとなるまで減圧濃縮した。次に、濃縮残渣にエタノール（４２ｋｇ，１．０ｗ／ｗ）と酢酸エチル（９６ｋｇ，２．２６ｗ／ｗ）を加え５分攪拌した後、５５～６５℃の温水循環下、減圧度－０．０９２ＭＰａ以上でほぼ留出を認めなくなるまで減圧濃縮した。ここで、結晶の析出を認めたため、結晶が完全に溶解するまで少しずつ酢酸エチルを加えた（１３．８５ｋｇ使用）。さらにエタノール（１８．３ｋｇ）及び酢酸エチル（６．７ｋｇ）を加えた後、内温を５０～５５℃に調整し、結晶が溶解していることを目視にて確認後、内温４５～５５℃でｎ－ヘプタン（３３．５ｋｇ，０．７９ｗ／ｗ）を３０分以上かけて滴下した。続いて、内温４５～５０℃で、国際公開第０８／１３６２７９号パンフレットの明細書実施例18に記載の方法で合成できる３－（３－（４－ピリジン－２－イルオキシメチル）－ベンジル）－イソキサゾール－５－イル）－ピリジン－２－イルアミン（０．０１１ｋｇ）を加え、結晶の析出を確認後、同温度範囲で１時間以上攪拌した。内温４５～５５℃でｎ－ヘプタン（６６．９ｋｇ，１．５８ｗ／ｗ）を１時間以上かけて滴下した後、４時間以上かけて内温０～１０℃まで冷却し、同温度範囲で５時間以上攪拌した。内容液をサンプリングし、目的物の結晶化率が９４％であることを確認した後、懸濁液を加圧ろ過し、結晶をエタノール－酢酸エチル－ｎ－ヘプタン混液（エタノール（３．６０ｋｇ，０．０８５ｗ／ｗ）と酢酸エチル（４．１５ｋｇ，０．０９８ｗ／ｗ）とｎ－ヘプタン（１８．８１ｋｇ，０．４４４ｗ／ｗ）の混液）、エタノール－ｎ－ヘプタン混液（エタノール（７．２５ｋｇ，０．１７１ｗ／ｗ）とｎ－ヘプタン（１８．８１ｋｇ，０．４４４ｗ／ｗ）の混液）の順にかけ洗いを行い、標記化合物のｗｅｔ粗結晶（３６．５２ｋｇ）を微黄色結晶として得た。
　あらかじめ窒素置換した５００Ｌ溶解缶に、得られた３－（３－（４－ピリジン－２－イルオキシメチル）－ベンジル）－イソキサゾール－５－イル）－ピリジン－２－イルアミンのｗｅｔ粗結晶（３６．５２ｋｇ）およびエタノール（５７．９ｋｇ，２．３７ｗ／ｗ）を順次加え、内温７０～７５℃まで加熱し、結晶を溶解させた。この溶解液を保温したままＳＵＳフィルターを通じて５００Ｌ晶析缶へ移送し、外温約６５℃で温めておいたエタノール（１９．３ｋｇ，０．８ｗ／ｗ）で５００Ｌ溶解缶及びＳＵＳフィルターを洗い込んだ。次に、ろ液を内温５５～６０℃に調整して、缶内の溶液が均一であることを確認した。その後、内温をゆっくりと４８～５１℃まで冷却したところ、結晶が析出した。再度、内温５５～６０℃まで加熱して結晶を溶解した後、速やかに内温４８～５１℃まで冷却し、直ちに、国際公開第０８／１３６２７９号パンフレットの明細書実施例18に記載の方法で合成できる３－（３－（４－ピリジン－２－イルオキシメチル）－ベンジル）－イソキサゾール－５－イル）－ピリジン－２－イルアミン（０．０１１ｋｇ）を加えた。続いて、内温４５～５０℃で結晶の析出を目視にて確認後、内温４３～４７℃で１時間～１時間３０分攪拌し、４時間以上かけて内温０～１０℃まで冷却した。ここで、析出した結晶をサンプリングし、その結晶形が標準品と同一であることを確認した後、同温度範囲で終夜攪拌した。翌日、結晶形が標準品と同一であることを確認した後、結晶を遠心分離機で２回に分けて固液分離し、それぞれエタノール１９．３ｋｇの約１／２量でかけ洗い、目的物のｗｅｔ結晶（２４．２３ｋｇ）を得た。このｗｅｔ結晶を混合型真空乾燥機に投入し、外温２０～３０℃で６時間以上、外温３５～４５℃で１２時間以上減圧乾燥し、標記化合物（２３．５２ｋｇ，６５．６３ｍｏｌ，収率：８６．８％）を淡黄色結晶として得た。
1H－NMR  Spectrum  (CDCl3)δ(ppm)：4.07(2H,s),  5.37(2H,s),　5.42(2H,brs),  6.25(1H,s),　6.71(1H,dd,J＝5.2,7.6Hz),  6.80(1H,d,J＝8.4Hz),  6.87－6.91(1H,m),  7.30(2H,d,J＝7.6Hz),  7.44(2H,d,J＝7.6Hz),  7.56－7.61(1H,m),  7.70(1H,dd,J＝2.0,7.6Hz),  8.14(1H,dd,J＝2.0,4.8Hz),  8.16－8.19(1H,m).
ＨＰＬＣ条件　カラム：CAPCELL  PAK　C18  MGII  (5μm,　150x4.6mmI.D.,　SHISEIDO)，移動相：アセトニトリル／水／トリフルオロ酢酸=１８０／８２０／１～９００／１００／１（ｖ／ｖ／ｖ）。 [Reference Example 33] 3- (3- (4-Pyridin-2-yloxymethyl) -benzyl) -isoxazol-5-yl) -pyridin-2-ylamine
Figure JPOXMLDOC01-appb-C000081
 Di-tert-butyl [3- (3- {4 [(pyridin-2-yloxy) methyl] benzyl} isoxazol-5-yl) pyridin-2-yl] imide dicarbonate in toluene solution (42.37 kg (75. 85 mol), formic acid (181 kg, 4.27 w / w) was added dropwise at an internal temperature of -5 to 20 ° C, and the mixture was stirred at an internal temperature of 22 to 32 ° C for 19 to 20 hours. After confirming the completion of the reaction by HPLC analysis, the reaction solution was cooled to an internal temperature of −5 to 10 ° C., and the contents were divided into two and added to 500 L reactors 1 and 2, respectively.
About 500L reaction can 1, the post-processing was implemented as follows. Under stirring, water (74 kg, 1.75 w / w) was added dropwise at an internal temperature of -5 to 20 ° C., and tert-butyl methyl ether (31.4 kg, 0.74 w / w) was added at an internal temperature of 0 to 25 ° C. n-Heptane (29.0 kg, 0.684 w / w) was added. The mixture was stirred at an internal temperature of 15 to 25 ° C. for 5 minutes and allowed to stand for 30 minutes or more to separate the lower layer. Return the lower layer to the can, and again add tert-butyl methyl ether (31.4 kg, 0.74 w / w) and n-heptane (29.0 kg, 0.684 w / w) at an internal temperature of 0 to 25 ° C. After stirring at 15 to 25 ° C. for 5 minutes, the mixture was allowed to stand for 30 minutes or more, and the lower layer was separated again. The lower layer was returned to the can, and a 48% aqueous sodium hydroxide solution (116 kg, 1392.0 mol, 18.35 M / M as sodium hydroxide) was first added dropwise at an internal temperature of 0 to 25 ° C. Next, ethyl acetate (96 kg, 2.26 w / w) was added in the same temperature range, and a 48% aqueous sodium hydroxide solution (20.5 kg, 246.0 mol as sodium hydroxide, 3.24 M / M) was added dropwise. Furthermore, about 8% sodium hydroxide aqueous solution (48% sodium hydroxide aqueous solution (12.7 kg, 152.4 mol as sodium hydroxide, 2.00 M / M)) and water (64 kg, 1.5 w / m) in the same temperature range. The mixture (w) was added dropwise until the lower layer had a pH of 8.00 to 9.00 (actual value: pH 8.58) (0.75 kg used). Thereafter, the mixture was stirred at an internal temperature of 20 to 30 ° C. for 1 hour or more and allowed to stand overnight, and then the pH of the lower layer was reconfirmed (actual measurement pH 8.29), and the lower layer was removed. About 5% aqueous sodium hydrogen carbonate solution (mixture of sodium hydrogen carbonate (5.3 kg, 63.09 mol) and water (101 kg, 2.375 w / w)) was added to the upper layer remaining in the can, and the internal temperature was 20-30 ° C. After stirring for 1 hour or longer, the mixture was allowed to stand for 30 minutes or longer. After removing the lower layer (pH 8.60), water (106 kg, 2.5 w / w) was added to the upper layer, stirred for 1 hour or more at an internal temperature of 20 to 30 ° C., and then allowed to stand for 30 minutes or more. pH 7.17) was removed.
About 500L reaction can 2, the same post-process was implemented in parallel with 500L reaction can 1.
The content liquid in the 500 L reaction vessel 1 was transferred to the 500 L reaction vessel 2 and concentrated under reduced pressure while circulating hot water at 55 to 65 ° C. until the content solution reached about 100 L. Next, ethanol (42 kg, 1.0 w / w) and ethyl acetate (96 kg, 2.26 w / w) were added to the concentrated residue, and the mixture was stirred for 5 minutes. The solution was concentrated under reduced pressure until no distillation was observed at 092 MPa or more. Here, since precipitation of crystals was observed, ethyl acetate was added little by little until the crystals were completely dissolved (13.85 kg used). Further, ethanol (18.3 kg) and ethyl acetate (6.7 kg) were added, the internal temperature was adjusted to 50 to 55 ° C., and it was visually confirmed that the crystals were dissolved, and then the internal temperature was 45 to 55. N-Heptane (33.5 kg, 0.79 w / w) was added dropwise at 30 ° C. over 30 minutes. Subsequently, 3- (3- (4-pyridin-2-yloxymethyl) -benzyl which can be synthesized at an internal temperature of 45 to 50 ° C. by the method described in the specification Example 18 of WO08 / 136279. ) -Isoxazol-5-yl) -pyridin-2-ylamine (0.011 kg) was added, and after confirming the precipitation of crystals, the mixture was stirred at the same temperature range for 1 hour or more. After dropping n-heptane (66.9 kg, 1.58 w / w) over 1 hour at an internal temperature of 45 to 55 ° C., the internal temperature was cooled to 0 to 10 ° C. over 4 hours. Stir for 5 hours or more. The content liquid was sampled, and it was confirmed that the crystallization rate of the target product was 94%. Then, the suspension was filtered under pressure, and the crystal was mixed with ethanol-ethyl acetate-n-heptane (ethanol (3.60 kg, 0.085 w / w), ethyl acetate (4.15 kg, 0.098 w / w) and n-heptane (18.81 kg, 0.444 w / w)), ethanol-n-heptane mixture (ethanol (7. 25 kg, 0.171 w / w) and n-heptane (18.81 kg, 0.444 w / w) in this order) and washed to obtain crude wet crystals (36.52 kg) of the title compound as pale yellow crystals. It was.
Into a 500-L dissolving can previously purged with nitrogen, the obtained wet crude crystals of 3- (3- (4-pyridin-2-yloxymethyl) -benzyl) -isoxazol-5-yl) -pyridin-2-ylamine (36 .52 kg) and ethanol (57.9 kg, 2.37 w / w) were sequentially added and heated to an internal temperature of 70 to 75 ° C. to dissolve the crystals. This dissolved solution was transferred to a 500 L crystallization can through a SUS filter while being kept warm, and the 500 L dissolving can and the SUS filter were washed with ethanol (19.3 kg, 0.8 w / w) heated at an external temperature of about 65 ° C. It is. Next, the filtrate was adjusted to an internal temperature of 55-60 ° C., and it was confirmed that the solution in the can was uniform. Thereafter, when the internal temperature was slowly cooled to 48 to 51 ° C., crystals were precipitated. After heating again to an internal temperature of 55 to 60 ° C. to dissolve the crystals, the crystal is promptly cooled to an internal temperature of 48 to 51 ° C., and immediately, the method described in the specification Example 18 of WO08 / 136279 3- (3- (4-Pyridin-2-yloxymethyl) -benzyl) -isoxazol-5-yl) -pyridin-2-ylamine (0.011 kg), which can be synthesized by: Subsequently, after visually confirming the precipitation of crystals at an internal temperature of 45 to 50 ° C., the mixture was stirred at an internal temperature of 43 to 47 ° C. for 1 hour to 1 hour and 30 minutes, and then cooled to an internal temperature of 0 to 10 ° C. over 4 hours. did. Here, the precipitated crystals were sampled, and after confirming that the crystal form was the same as the standard product, the crystals were stirred overnight in the same temperature range. The next day, after confirming that the crystal form was the same as that of the standard product, the crystal was separated into solid and liquid by centrifuge twice and washed with about 1/2 amount of 19.3 kg of ethanol. A wet crystal (24.23 kg) was obtained. The wet crystals were put into a mixed vacuum dryer and dried under reduced pressure at an external temperature of 20 to 30 ° C. for 6 hours or longer and at an external temperature of 35 to 45 ° C. for 12 hours or longer to obtain the title compound (23.52 kg, 65.63 mol, yield). Ratio: 86.8%) was obtained as pale yellow crystals.
1 H-NMR Spectrum (CDCl 3 ) δ (ppm): 4.07 (2H, s), 5.37 (2H, s), 5.42 (2H, brs), 6.25 (1H, s), 6.71 (1H, dd, J = 5.2,7.6Hz), 6.80 (1H, d, J = 8.4Hz), 6.87-6.91 (1H, m), 7.30 (2H, d, J = 7.6Hz), 7.44 (2H, d, J = 7.6Hz) 7.56-7.61 (1H, m), 7.70 (1H, dd, J = 2.0,7.6Hz), 8.14 (1H, dd, J = 2.0,4.8Hz), 8.16-8.19 (1H, m).
HPLC conditions Column: CAPCELL PAK C18 MGII (5 μm, 150 × 4.6 mm I.D., SHISEIDO), mobile phase: acetonitrile / water / trifluoroacetic acid = 180/820/1 to 900/100/1 (v / v / v) .
［実施例１］（Ｓ）－２－アミノ－４－（３－（３－（４－（ピリジン－２－イルオキシメチル）－ベンジル）－イソキサゾール－５－イル）－ピリジン－２－イルカルバモイル）－ブチリック　アシド Example 1 (S) -2-Amino-4- (3- (3- (4- (pyridin-2-yloxymethyl) -benzyl) -isoxazol-5-yl) -pyridin-2-ylcarbamoyl )-Butyric Acid
Figure JPOXMLDOC01-appb-C000082
Figure JPOXMLDOC01-appb-C000082
　製造例１－１に記載の（Ｓ）－２－ｔ－ブトキシカルボニルアミノ－４－（３－（３－（４－（ピリジン－２－イルオキシメチル）－ベンジル）－イソキサゾール－５－イル）－ピリジン－２－イルカルバモイル）－ブチリック　アシド　ｔ－ブチル　エステル（９８０ｍｇ、１．５ｍｍｏｌ）とジクロロメタン（１０ｍＬ）の混合物に、０℃でトリフルオロ酢酸（１０ｍＬ）を加え、室温で終夜攪拌した。溶媒を減圧下留去し、残渣をジエチルエーテルで洗浄し、標記化合物（８５０ｍｇ）をトリフルオロ酢酸塩として得た。
1H－NMR  Spectrum  (DMSO－d6)δ(ppm)：　1.91-2.05(2H,m),　2.44-2.57(2H,m),　3.91(1H,brs),  4.03(2H,s),　5.32(2H,s),  6.66(1H,s),　6.84-6.87(1H,m),　6.98-7.01(1H,m),　7.31(2H,d,J=8.1Hz),  7.40-7.44(3H,m),  7.70-7.75(1H,m),  8.16-8.18(2H,m),  8.26(3H,brs),　8.52(1H,dd,J=1.8,4.7Hz),　10.49(1H,s). (S) -2-t-butoxycarbonylamino-4- (3- (3- (4- (pyridin-2-yloxymethyl) -benzyl) -isoxazol-5-yl) described in Preparation Example 1-1 To a mixture of -pyridin-2-ylcarbamoyl) -butyric acid t-butyl ester (980 mg, 1.5 mmol) and dichloromethane (10 mL) was added trifluoroacetic acid (10 mL) at 0 ° C., and the mixture was stirred at room temperature overnight. The solvent was distilled off under reduced pressure, and the residue was washed with diethyl ether to obtain the title compound (850 mg) as a trifluoroacetate salt.
1 H-NMR Spectrum (DMSO-d 6 ) δ (ppm): 1.91-2.05 (2H, m), 2.44-2.57 (2H, m), 3.91 (1H, brs), 4.03 (2H, s), 5.32 ( 2H, s), 6.66 (1H, s), 6.84-6.87 (1H, m), 6.98-7.01 (1H, m), 7.31 (2H, d, J = 8.1Hz), 7.40-7.44 (3H, m) , 7.70-7.75 (1H, m), 8.16-8.18 (2H, m), 8.26 (3H, brs), 8.52 (1H, dd, J = 1.8,4.7Hz), 10.49 (1H, s).
　出発物質（Ｓ）－２－ｔ－ブトキシカルボニルアミノ－４－（３－（３－（４－（ピリジン－２－イルオキシメチル）－ベンジル）－イソキサゾール－５－イル）－ピリジン－２－イルカルバモイル）－ブチリック　アシド　ｔ－ブチル　エステルは以下の方法で合成した。 Starting material (S) -2-t-butoxycarbonylamino-4- (3- (3- (4- (pyridin-2-yloxymethyl) -benzyl) -isoxazol-5-yl) -pyridin-2-y Rucarbamoyl) -butyric acid t-butyl ester was synthesized by the following method.
［製造例１－１］（Ｓ）－２－ｔ－ブトキシカルボニルアミノ－４－（３－（３－（４－（ピリジン－２－イルオキシメチル）－ベンジル）－イソキサゾール－５－イル）－ピリジン－２－イルカルバモイル）－ブチリック　アシド　ｔ－ブチル　エステル [Production Example 1-1] (S) -2-t-butoxycarbonylamino-4- (3- (3- (4- (pyridin-2-yloxymethyl) -benzyl) -isoxazol-5-yl)- Pyridin-2-ylcarbamoyl) -butyric acid t-butyl ester
Figure JPOXMLDOC01-appb-C000083
Figure JPOXMLDOC01-appb-C000083
　参考例１に記載の３－（３－（４－（ピリジン－２－イルオキシメチル）－ベンジル）－イソキサゾール－５－イル）－ピリジン－２－イルアミン（９００ｍｇ、２．５ｍｍｏｌ）のアセトニトリル（１５ｍＬ）溶液に、０℃で（Ｓ）－２－ｔ－ブトキシカルボニルアミノ－ペンタンジオイック　アシド　１－ｔ－ブチル　エステル（８６０ｍｇ、２．８ｍｍｏｌ）、トリエチルアミン（０．３７ｍＬ、２．６ｍｍｏｌ）、及びＯ－（７－アザベンゾトリアゾール－１－イル）－Ｎ，Ｎ，Ｎ'，Ｎ’－テトラメチルウロニウム　ヘキサフルオロホスフェート（１．１ｇ、２．８ｍｍｏｌ）を加え、６０℃で終夜攪拌した。反応溶液に水と酢酸エチルを加え、有機層を飽和食塩水で洗浄した。得られた残渣をＮＨ－シリカゲルカラムクロマトグラフィー（ヘプタン：酢酸エチル＝４：１）にて精製し、標記化合物（９８０ｍｇ）を得た。
1H－NMR  Spectrum  (CDCl3)δ(ppm)：　1.42(9H,s),  1.46(9H,s),　1.95(1H,brs),  2.23(1H,brs),　2.64-2.66(2H,m),　4.08(2H,s),  4.23(1H,brs), 5.27(1H,d,J=7.1Hz),　5.36(2H,s),  6.33(1H,s),　6.71-6.81(1H,m),　6.87-6.90(1H,m),　7.18(1H,dd,J=4.7,7.8Hz),　7.30(2H,d,J=8.1Hz),  7.44(2H,d,J=8.1Hz),　7.56-7.61(1H,m),　7.93(1H,d,J=7.9Hz),  8.16-8.18(1H,m),  8.47-8.48(1H,m),  8.52(1H,brs). 3- (3- (4- (Pyridin-2-yloxymethyl) -benzyl) -isoxazol-5-yl) -pyridin-2-ylamine (900 mg, 2.5 mmol) described in Reference Example 1 in acetonitrile (15 mL ) Solution at 0 ° C. with (S) -2-t-butoxycarbonylamino-pentanedioic acid 1-t-butyl ester (860 mg, 2.8 mmol), triethylamine (0.37 mL, 2.6 mmol), and O -(7-Azabenzotriazol-1-yl) -N, N, N ′, N′-tetramethyluronium hexafluorophosphate (1.1 g, 2.8 mmol) was added, and the mixture was stirred at 60 ° C. overnight. Water and ethyl acetate were added to the reaction solution, and the organic layer was washed with saturated brine. The obtained residue was purified by NH-silica gel column chromatography (heptane: ethyl acetate = 4: 1) to obtain the title compound (980 mg).
1 H-NMR Spectrum (CDCl 3 ) δ (ppm): 1.42 (9H, s), 1.46 (9H, s), 1.95 (1H, brs), 2.23 (1H, brs), 2.64-2.66 (2H, m) , 4.08 (2H, s), 4.23 (1H, brs), 5.27 (1H, d, J = 7.1Hz), 5.36 (2H, s), 6.33 (1H, s), 6.71-6.81 (1H, m), 6.87-6.90 (1H, m), 7.18 (1H, dd, J = 4.7,7.8Hz), 7.30 (2H, d, J = 8.1Hz), 7.44 (2H, d, J = 8.1Hz), 7.56-7.61 (1H, m), 7.93 (1H, d, J = 7.9Hz), 8.16-8.18 (1H, m), 8.47-8.48 (1H, m), 8.52 (1H, brs).
［実施例２］（Ｓ）－２－アミノ－４－（３－（３－（４－（ピリジン－２－イルオキシメチル）－ベンジル）－イソキサゾール―５－イル）－ピリジン―２－イルカルバモイル）－ブチリック　アシド　メチル　エステル Example 2 (S) -2-Amino-4- (3- (3- (4- (pyridin-2-yloxymethyl) -benzyl) -isoxazol-5-yl) -pyridin-2-ylcarbamoyl ) -Butyric acid methyl ester
Figure JPOXMLDOC01-appb-C000084
 　製造例２－２に記載の（Ｓ）－２－ｔ－ブトキシカルボニルアミノ－４－（３－（３－（４－（ピリジン－２－イルオキシメチル）－ベンジル）－イソキサゾール―５－イル）－ピリジン―２－イルカルバモイル）－ブチリック　アシド　メチル　エステル（３１ｍｇ、０．０５２ｍｍｏｌ）とジクロロメタン（１ｍＬ）の混合物に、室温でトリフルオロ酢酸（０．５ｍＬ）を加え、同温で終夜攪拌した。反応混合物を減圧下溶媒留去し、得られた残渣をジエチルエーテルで洗浄し、標記化合物（２６ｍｇ）をトリフルオロ酢酸塩として得た。
1H－NMR  Spectrum  (DMSO－d6)δ(ppm)：　1.97-2.03(2H,m),　2.43-2.53(2H,m),　3.74(3H,s),  4.04-4.05(3H,m),  5.32(2H,s),　6.66(1H,s),  6.84-6.87(1H,m),  6.98-7.01(1H,m),  7.31(2H,d,J=8.2Hz),　7.41(2H,d,J=8.1Hz),  7.43(1H,d,J=7.9Hz),　7.70-7.75(1H,m), 8.16(1H,d,J=1.8Hz),　8.18(1H,t,J=1.8Hz),  8.38(3H,brs),　8.52(1H,dd,J=1.8,4.8Hz),　10.47(1H,s).
Figure JPOXMLDOC01-appb-C000084
 (S) -2-t-butoxycarbonylamino-4- (3- (3- (4- (pyridin-2-yloxymethyl) -benzyl) -isoxazol-5-yl) described in Preparation Example 2-2 To a mixture of -pyridin-2-ylcarbamoyl) -butyric acid methyl ester (31 mg, 0.052 mmol) and dichloromethane (1 mL) was added trifluoroacetic acid (0.5 mL) at room temperature, and the mixture was stirred at the same temperature overnight. The reaction mixture was evaporated under reduced pressure, and the obtained residue was washed with diethyl ether to give the title compound (26 mg) as a trifluoroacetate salt.
1 H-NMR Spectrum (DMSO-d 6 ) δ (ppm): 1.97-2.03 (2H, m), 2.43-2.53 (2H, m), 3.74 (3H, s), 4.04-4.05 (3H, m), 5.32 (2H, s), 6.66 (1H, s), 6.84-6.87 (1H, m), 6.98-7.01 (1H, m), 7.31 (2H, d, J = 8.2Hz), 7.41 (2H, d, J = 8.1Hz), 7.43 (1H, d, J = 7.9Hz), 7.70-7.75 (1H, m), 8.16 (1H, d, J = 1.8Hz), 8.18 (1H, t, J = 1.8Hz) , 8.38 (3H, brs), 8.52 (1H, dd, J = 1.8,4.8Hz), 10.47 (1H, s).
　出発物質（Ｓ）－２－ｔ－ブトキシカルボニルアミノ－４－（３－（３－（４－（ピリジン－２－イルオキシメチル）－ベンジル）－イソキサゾール―５－イル）－ピリジン―２－イルカルバモイル）－ブチリック　アシド　メチル　エステルは以下の方法で合成した。 Starting material (S) -2-t-butoxycarbonylamino-4- (3- (3- (4- (pyridin-2-yloxymethyl) -benzyl) -isoxazol-5-yl) -pyridin-2-y Rucarbamoyl) -butyric acid methyl ester was synthesized by the following method.
［製造例２－１］（Ｓ）－２－ｔ－ブトキシカルボニルアミノ－ペンタンジオイック　アシド　５－ベンジル　エステル　１－メチルエステル [Production Example 2-1] (S) -2-t-butoxycarbonylamino-pentanedioic acid 5-benzyl ester 1-methyl ester
Figure JPOXMLDOC01-appb-C000085
 　（Ｓ）－２－ｔ－ブトキシカルボニルアミノ－ペンタンジオイック　アシド　５－ベンジル　エステル（３００ｍｇ、０．８９ｍｍｏｌ）とメタノール（４ｍＬ）の混合物に、０℃でベンゾトリアゾール－１－イルオキシトリス－（ジメチルアミノ）－ホスホニウム　ヘキサフルオロホスフェート（３９０ｍｇ、０．８９ｍｍｏｌ）、Ｎ－メチルモルホリン（９８μＬ、０．８９ｍｍｏｌ）、及びアセトニトリル（１ｍＬ）を加え、室温で終夜攪拌した。反応混合物に１Ｎ水酸化ナトリウム水溶液を加え、酢酸エチルで抽出した。有機層を水及び飽和食塩水で順次洗浄し、無水硫酸マグネシウムで乾燥した。減圧下溶媒留去し、標記化合物（３８０ｍｇ、純度６６％）を粗体として得た。
1H－NMR  Spectrum  (CDCl3)δ(ppm)：　1.43(9H,s),  1.92-2.01(1H,m),  2.18-2.23(1H,m),  2.39-2.51(2H,m),  3.73(3H,s),　4.34-4.36(1H,m),　5.10-5.12(3H,m),　7.31-7.39(5H,m).
Figure JPOXMLDOC01-appb-C000085
 To a mixture of (S) -2-t-butoxycarbonylamino-pentanedioic acid 5-benzyl ester (300 mg, 0.89 mmol) and methanol (4 mL) at 0 ° C., benzotriazol-1-yloxytris- (dimethyl) Amino) -phosphonium hexafluorophosphate (390 mg, 0.89 mmol), N-methylmorpholine (98 μL, 0.89 mmol), and acetonitrile (1 mL) were added and stirred overnight at room temperature. To the reaction mixture was added 1N aqueous sodium hydroxide solution, and the mixture was extracted with ethyl acetate. The organic layer was washed successively with water and saturated brine, and dried over anhydrous magnesium sulfate. The solvent was distilled off under reduced pressure to obtain the title compound (380 mg, purity 66%) as a crude product.
1 H-NMR Spectrum (CDCl 3 ) δ (ppm): 1.43 (9H, s), 1.92-2.01 (1H, m), 2.18-2.23 (1H, m), 2.39-2.51 (2H, m), 3.73 ( 3H, s), 4.34-4.36 (1H, m), 5.10-5.12 (3H, m), 7.31-7.39 (5H, m).
［製造例２－２］（Ｓ）－２－ｔ－ブトキシカルボニルアミノ－４－（３－（３－（４－（ピリジン－２－イルオキシメチル）－ベンジル）－イソキサゾール―５－イル）－ピリジン―２－イルカルバモイル）－ブチリック　アシド　メチル　エステル [Production Example 2-2] (S) -2-t-butoxycarbonylamino-4- (3- (3- (4- (pyridin-2-yloxymethyl) -benzyl) -isoxazol-5-yl)- Pyridin-2-ylcarbamoyl) -butyric acid methyl ester
Figure JPOXMLDOC01-appb-C000086
 　製造例２－１に記載の（Ｓ）－２－ｔ－ブトキシカルボニルアミノ－ペンタンジオイック　アシド　５－ベンジル　エステル　１－メチルエステル（純度６６％、３７０ｍｇ、０．７０ｍｍｏｌ）とメタノール（４ｍＬ）の混合物に、室温でパラジウム－カーボン（５０％含水、４０ｍｇ、０．１９ｍｍｏｌ）を加え、水素雰囲気下（１ａｔｍ）、室温で５時間攪拌した。反応混合物を窒素で置換し、セライトを用いてろ過した。ろ液を減圧下溶媒留去し、（Ｓ）－２－ｔ－ブトキシカルボニルアミノ－ペンタンジオイック　アシド　１－メチル　エステル　（３００ｍｇ）を粗体として得た。得られた粗体をそのまま次の反応に用いた。参考例１に記載の３－（３－（４－（ピリジン－２－イルオキシメチル）－ベンジル）－イソキサゾール－５－イル）－ピリジン－２－イルアミン（１５０ｍｇ、０．４２ｍｍｏｌ）とアセトニトリル（３ｍＬ）の混合物に、０℃で（Ｓ）－２－ｔ－ブトキシカルボニルアミノ－ペンタンジオイック　アシド　１－メチル　エステル　（粗体、２２０ｍｇ）、Ｎ－メチルモルホリン（４６μＬ、０．４２ｍｍｏｌ）、Ｏ－（７－アザベンゾトリアゾール－１－イル）－１、１、３、３－テトラメチルウロミウム　ヘキサフルオロホスフェート（１８０ｍｇ、０．４６ｍｍｏｌ）を順次加え、室温で終夜攪拌した。反応混合物に水を加え、酢酸エチルで抽出した。有機層を飽和食塩水で洗浄し、減圧下溶媒留去した。残渣をＮＨシリカゲルカラムクロマトグラフィー（酢酸エチル：ヘプタン＝４：１）で精製し、標記化合物（３７ｍｇ）を得た。
1H－NMR  Spectrum  (CDCl3)δ(ppm)：　1.42(9H,s),  1.97-2.04(1H,m),  2.22-2.31(1H,m),  2.67-2.70(2H,m),  3.73(3H,s),　4.08(2H,s),  4.36-4.37(1H,m), 5.34-5.36(3H,m),　 6.34(1H,s),　6.79-6.81(1H,m),　6.87-6.90(1H,m),　7.18(1H,dd,J=4.8,7.9Hz),　7.30(2H,d,J=8.1Hz),  7.44(2H,d,J=8.1Hz),　7.56-7.61(1H,m),　7.94(1H,dd,J=1.8,7.8Hz),　8.16-8.18(1H,m),　8.47-8.48(2H,m).
Figure JPOXMLDOC01-appb-C000086
 A mixture of (S) -2-t-butoxycarbonylamino-pentanedioic acid 5-benzyl ester 1-methyl ester (purity 66%, 370 mg, 0.70 mmol) and methanol (4 mL) described in Preparation Example 2-1. To the mixture was added palladium-carbon (50% water content, 40 mg, 0.19 mmol) at room temperature, and the mixture was stirred at room temperature for 5 hours in a hydrogen atmosphere (1 atm). The reaction mixture was purged with nitrogen and filtered through celite. The filtrate was evaporated under reduced pressure to give (S) -2-t-butoxycarbonylamino-pentanedioic acid 1-methyl ester (300 mg) as a crude product. The obtained crude product was directly used in the next reaction. 3- (3- (4- (Pyridin-2-yloxymethyl) -benzyl) -isoxazol-5-yl) -pyridin-2-ylamine (150 mg, 0.42 mmol) and acetonitrile (3 mL) described in Reference Example 1 ) Was added at 0 ° C. to (S) -2-t-butoxycarbonylamino-pentanedioic acid 1-methyl ester (crude, 220 mg), N-methylmorpholine (46 μL, 0.42 mmol), O- ( 7-azabenzotriazol-1-yl) -1,1,3,3-tetramethylurmium hexafluorophosphate (180 mg, 0.46 mmol) was sequentially added, and the mixture was stirred at room temperature overnight. Water was added to the reaction mixture, and the mixture was extracted with ethyl acetate. The organic layer was washed with saturated brine, and the solvent was distilled off under reduced pressure. The residue was purified by NH silica gel column chromatography (ethyl acetate: heptane = 4: 1) to obtain the title compound (37 mg).
1 H-NMR Spectrum (CDCl 3 ) δ (ppm): 1.42 (9H, s), 1.97-2.04 (1H, m), 2.22-2.31 (1H, m), 2.67-2.70 (2H, m), 3.73 ( 3H, s), 4.08 (2H, s), 4.36-4.37 (1H, m), 5.34-5.36 (3H, m), 6.34 (1H, s), 6.79-6.81 (1H, m), 6.87-6.90 ( 1H, m), 7.18 (1H, dd, J = 4.8,7.9Hz), 7.30 (2H, d, J = 8.1Hz), 7.44 (2H, d, J = 8.1Hz), 7.56-7.61 (1H, m ), 7.94 (1H, dd, J = 1.8,7.8Hz), 8.16-8.18 (1H, m), 8.47-8.48 (2H, m).
［実施例３］（Ｓ）－２－アミノ－４－（３－（３－（４－（ピリジン－２－イルオキシメチル）－ベンジル）－イソキサゾール―５－イル）－ピリジン―２－イルカルバモイル）－ブチリック　アシド　２－ジメチルアミノ－エチル　エステル Example 3 (S) -2-Amino-4- (3- (3- (4- (pyridin-2-yloxymethyl) -benzyl) -isoxazol-5-yl) -pyridin-2-ylcarbamoyl ) -Butyric acid 2-dimethylamino-ethyl ester
Figure JPOXMLDOC01-appb-C000087
 　製造例３－２に記載の（Ｓ）－２－ｔ－ブトキシカルボニルアミノ－４－（３－（３－（４－（ピリジン－２－イルオキシメチル）－ベンジル）－イソキサゾール―５－イル）－ピリジン―２－イルカルバモイル）－ブチリック　アシド　２－ジメチルアミノ－エチル　エステル（純度７５％、５３ｍｇ、０．０６０ｍｍｏｌ）とジクロロメタン（１ｍＬ）の混合物に、室温でトリフルオロ酢酸（０．５ｍＬ）を加え、同温で終夜攪拌した。反応混合物を減圧下溶媒留去し、得られた残渣をジエチルエーテルで洗浄し、標記化合物（４６ｍｇ）を２トリフルオロ酢酸塩として得た。
1H－NMR  Spectrum  (DMSO－d6)δ(ppm)：　2.06(2H,brs),  2.49-2.54(2H,m),  2.84(6H,s),　3.44(2H,brs),  4.04-4.08(3H,m),  4.44-4.47(2H,m),  5.32(2H,s),　6.69(1H,s),  6.85(1H,dd,J=0.7,8.4Hz),  6.98-7.01(1H,m),  7.31(2H,d,J=8.1Hz),　7.41-7.45(3H,m),　7.70-7.75(1H,m),　8.16-8.19(2H,m),　8.52-8.53(4H,m),　10.48(1H,s).
Figure JPOXMLDOC01-appb-C000087
 (S) -2-t-butoxycarbonylamino-4- (3- (3- (4- (pyridin-2-yloxymethyl) -benzyl) -isoxazol-5-yl) described in Preparation Example 3-2 To a mixture of -pyridin-2-ylcarbamoyl) -butyric acid 2-dimethylamino-ethyl ester (purity 75%, 53 mg, 0.060 mmol) and dichloromethane (1 mL) was added trifluoroacetic acid (0.5 mL) at room temperature. And stirred at the same temperature overnight. The reaction mixture was evaporated under reduced pressure, and the obtained residue was washed with diethyl ether to give the title compound (46 mg) as 2 trifluoroacetate.
1 H-NMR Spectrum (DMSO-d 6 ) δ (ppm): 2.06 (2H, brs), 2.49-2.54 (2H, m), 2.84 (6H, s), 3.44 (2H, brs), 4.04-4.08 ( 3H, m), 4.44-4.47 (2H, m), 5.32 (2H, s), 6.69 (1H, s), 6.85 (1H, dd, J = 0.7,8.4Hz), 6.98-7.01 (1H, m) , 7.31 (2H, d, J = 8.1Hz), 7.41-7.45 (3H, m), 7.70-7.75 (1H, m), 8.16-8.19 (2H, m), 8.52-8.53 (4H, m), 10.48 (1H, s).
　出発物質（Ｓ）－２－ｔ－ブトキシカルボニルアミノ－４－（３－（３－（４－（ピリジン－２－イルオキシメチル）－ベンジル）－イソキサゾール―５－イル）－ピリジン―２－イルカルバモイル）－ブチリック　アシド　２－ジメチルアミノ－エチル　エステルは以下の方法で合成した。 Starting material (S) -2-t-butoxycarbonylamino-4- (3- (3- (4- (pyridin-2-yloxymethyl) -benzyl) -isoxazol-5-yl) -pyridin-2-y Rucarbamoyl) -butyric acid 2-dimethylamino-ethyl ester was synthesized by the following method.
［製造例３－１］（Ｓ）－２－ｔ－ブトキシカルボニルアミノ－ペンタンジオイック　アシド　５－ベンジル　エステル　１－（２－ジメチルアミノ－エチル）　エステル [Production Example 3-1] (S) -2-t-Butoxycarbonylamino-pentanedioic acid 5-benzyl ester 1- (2-dimethylamino-ethyl) ester
Figure JPOXMLDOC01-appb-C000088
 　（Ｓ）－２－ｔ－ブトキシカルボニルアミノ－ペンタンジオイック　アシド　５－ベンジル　エステル（３００ｍｇ、０．８９ｍｍｏｌ）とアセトニトリル（５ｍＬ）の混合物に、０℃で２－ジメチルアミノエタノール（８９μＬ、０．８９ｍｍｏｌ）及びベンゾトリアゾール－１－イルオキシトリス－（ジメチルアミノ）－ホスホニウム　ヘキサフルオロホスフェート（３９０ｍｇ、０．８９ｍｍｏｌ）を加え、室温で終夜攪拌した。反応混合物に１Ｎ水酸化ナトリウム水溶液を加え、酢酸エチルで抽出した。有機層を水及び飽和食塩水で順次洗浄し、無水硫酸マグネシウムで乾燥した。減圧下溶媒留去し、標記化合物（３８０ｍｇ、純度７７％）を得た。
1H－NMR  Spectrum  (CDCl3)δ(ppm)：　1.43(9H,s),  1.94-2.03(1H,m),  2.18-2.25(1H,m),  2.28(6H,s),　2.41-2.55(2H,m),　2.59(2H,t,J=5.8Hz),  4.23-4.26(2H,m),  4.34-4.36(1H,m),  5.12(2H,s),　5.16-5.18(1H,m),　7.32-7.38(5H,m).
Figure JPOXMLDOC01-appb-C000088
 To a mixture of (S) -2-t-butoxycarbonylamino-pentanedioic acid 5-benzyl ester (300 mg, 0.89 mmol) and acetonitrile (5 mL) at 0 ° C. was added 2-dimethylaminoethanol (89 μL, 0.89 mmol). ) And benzotriazol-1-yloxytris- (dimethylamino) -phosphonium hexafluorophosphate (390 mg, 0.89 mmol) were added and stirred at room temperature overnight. To the reaction mixture was added 1N aqueous sodium hydroxide solution, and the mixture was extracted with ethyl acetate. The organic layer was washed successively with water and saturated brine, and dried over anhydrous magnesium sulfate. The solvent was distilled off under reduced pressure to obtain the title compound (380 mg, purity 77%).
1 H-NMR Spectrum (CDCl 3 ) δ (ppm): 1.43 (9H, s), 1.94-2.03 (1H, m), 2.18-2.25 (1H, m), 2.28 (6H, s), 2.41-2.55 ( 2H, m), 2.59 (2H, t, J = 5.8Hz), 4.23-4.26 (2H, m), 4.34-4.36 (1H, m), 5.12 (2H, s), 5.16-5.18 (1H, m) , 7.32-7.38 (5H, m).
［製造例３－２］（Ｓ）－２－ｔ－ブトキシカルボニルアミノ－４－（３－（３－（４－（ピリジン－２－イルオキシメチル）－ベンジル）－イソキサゾール―５－イル）－ピリジン―２－イルカルバモイル）－ブチリック　アシド　２－ジメチルアミノ－エチル　エステル [Production Example 3-2] (S) -2-t-butoxycarbonylamino-4- (3- (3- (4- (pyridin-2-yloxymethyl) -benzyl) -isoxazol-5-yl)- Pyridin-2-ylcarbamoyl) -butyric acid 2-dimethylamino-ethyl ester
Figure JPOXMLDOC01-appb-C000089
 　製造例３－１に記載の（Ｓ）－２－ｔ－ブトキシカルボニルアミノ－ペンタンジオイック　アシド　５－ベンジル　エステル　１－（２－ジメチルアミノ－エチル）　エステル（純度７７％、３７０ｍｇ、０．７０ｍｍｏｌ）とエタノール（４ｍＬ）の混合物に、室温でパラジウム－カーボン（５０％含水、４０ｍｇ、０．１９ｍｍｏｌ）を加え、水素雰囲気下（１ａｔｍ）、室温で終夜攪拌した。反応混合物を窒素で置換し、セライトを用いてろ過した。ろ液を減圧下溶媒留去し、（Ｓ）－２－ｔ－ブトキシカルボニルアミノ－ペンタンジオイック　アシド　１－（２－ジメチルアミノ－エチル）　エステル　（３００ｍｇ）を粗体として得た。得られた粗体をそのまま次の反応に用いた。参考例１に記載の３－（３－（４－（ピリジン－２－イルオキシメチル）－ベンジル）－イソキサゾール－５－イル）－ピリジン－２－イルアミン（１５０ｍｇ、０．４２ｍｍｏｌ）とアセトニトリル（３ｍＬ）の混合物に、０℃で（Ｓ）－２－ｔ－ブトキシカルボニルアミノ－ペンタンジオイック　アシド　１－（２－ジメチルアミノ－エチル）　エステル　（粗体、１９０ｍｇ）、Ｏ－（７－アザベンゾトリアゾール－１－イル）－１、１、３、３－テトラメチルウロミウム　ヘキサフルオロホスフェート（１６０ｍｇ、０．４２ｍｍｏｌ）を順次加え、室温で終夜攪拌した。反応混合物に水を加え、酢酸エチルで抽出した。有機層を飽和食塩水で洗浄し、減圧下溶媒留去した。残渣をＮＨシリカゲルカラムクロマトグラフィー（酢酸エチル：メタノール＝３０：１）で精製し、標記化合物（５６ｍｇ、純度７５％）を得た。
1H－NMR  Spectrum  (CDCl3)δ(ppm)：　1.41(9H,s),  2.12-2.14 (2H,m),　2.20(6H,s),  2.46-2.60(4H,m),  4.08-4.13(3H,m),  4.31-4.37(2H,m), 5.31-5.36(3H,m),　 6.37(1H,s),　6.78-6.81(1H,m),　6.87-6.90(1H,m),　7.21(1H,dd,J=4.8,7.9Hz),　7.30(2H,d,J=7.9Hz),  7.43(2H,d,J=8.2Hz),　7.56-7.61(1H,m),　7.98(1H,d,J=7.7Hz),  8.16-8.18(1H,m),  8.48(1H,dd,J=1.8,4.8Hz),  9.29(1H,brs).
Figure JPOXMLDOC01-appb-C000089
 (S) -2-t-Butoxycarbonylamino-pentanedioic acid 5-benzyl ester 1- (2-dimethylamino-ethyl) ester described in Preparation Example 3-1 (purity 77%, 370 mg, 0.70 mmol) To a mixture of ethanol and ethanol (4 mL) was added palladium-carbon (50% water content, 40 mg, 0.19 mmol) at room temperature, and the mixture was stirred at room temperature overnight under a hydrogen atmosphere (1 atm). The reaction mixture was purged with nitrogen and filtered through celite. The filtrate was evaporated under reduced pressure to give (S) -2-t-butoxycarbonylamino-pentanedioic acid 1- (2-dimethylamino-ethyl) ester (300 mg) as a crude product. The obtained crude product was directly used in the next reaction. 3- (3- (4- (Pyridin-2-yloxymethyl) -benzyl) -isoxazol-5-yl) -pyridin-2-ylamine (150 mg, 0.42 mmol) and acetonitrile (3 mL) described in Reference Example 1 (S) -2-t-butoxycarbonylamino-pentanedioic acid 1- (2-dimethylamino-ethyl) ester (crude, 190 mg), O- (7-azabenzotriazole) at 0 ° C. -1-yl) -1,1,3,3-tetramethylurmium hexafluorophosphate (160 mg, 0.42 mmol) was sequentially added, and the mixture was stirred at room temperature overnight. Water was added to the reaction mixture, and the mixture was extracted with ethyl acetate. The organic layer was washed with saturated brine, and the solvent was distilled off under reduced pressure. The residue was purified by NH silica gel column chromatography (ethyl acetate: methanol = 30: 1) to obtain the title compound (56 mg, purity 75%).
1 H-NMR Spectrum (CDCl 3 ) δ (ppm): 1.41 (9H, s), 2.12-2.14 (2H, m), 2.20 (6H, s), 2.46-2.60 (4H, m), 4.08-4.13 ( 3H, m), 4.31-4.37 (2H, m), 5.31-5.36 (3H, m), 6.37 (1H, s), 6.78-6.81 (1H, m), 6.87-6.90 (1H, m), 7.21 ( 1H, dd, J = 4.8,7.9Hz), 7.30 (2H, d, J = 7.9Hz), 7.43 (2H, d, J = 8.2Hz), 7.56-7.61 (1H, m), 7.98 (1H, d , J = 7.7Hz), 8.16-8.18 (1H, m), 8.48 (1H, dd, J = 1.8,4.8Hz), 9.29 (1H, brs).
［実施例４］（Ｓ）－２－アミノ－４－（３－（３－（４－（ピリジン－２－イルオキシメチル）－ベンジル）－イソキサゾール―５－イル）－ピリジン―２－イルカルバモイル）－ブチリック　アシド　エチル　エステル Example 4 (S) -2-Amino-4- (3- (3- (4- (pyridin-2-yloxymethyl) -benzyl) -isoxazol-5-yl) -pyridin-2-ylcarbamoyl ) -Butyric acid ethyl ester
Figure JPOXMLDOC01-appb-C000090
 　製造例４－２に記載の（Ｓ）－２－ｔ－ブトキシカルボニルアミノ－４－（３－（３－（４－（ピリジン－２－イルオキシメチル）－ベンジル）－イソキサゾール―５－イル）－ピリジン―２－イルカルバモイル）－ブチリック　アシド　エチル　エステル（４０ｍｇ、０．０６５ｍｍｏｌ）とジクロロメタン（１ｍＬ）の混合物に、室温でトリフルオロ酢酸（０．５ｍＬ）を加え、同温で終夜攪拌した。反応混合物を減圧下溶媒留去し、得られた残渣をジエチルエーテルで洗浄し、標記化合物（３０ｍｇ）をトリフルオロ酢酸塩として得た。
1H－NMR  Spectrum  (DMSO－d6)δ(ppm)：　1.24(3H,t,J=7.1Hz),  1.97-2.03(2H,m),  2.41-2.56(2H,m),  4.01-4.03(3H,m),  4.21(2H,q,J=7.1Hz),　5.32(2H,s),  6.66(1H,s),　6.84-6.86(1H,m),　6.98-7.01(1H,m),　7.30(2H,d,J=8.2Hz),  7.41(2H,d,J=8.1Hz),　7.43(1H,d,J=7.7Hz),  7.70-7.75(1H,m), 8.16-8.18(2H,m),　8.38(3H,brs),  8.52(1H,dd,J=1.8,4.8Hz),  10.48(1H,s).
Figure JPOXMLDOC01-appb-C000090
 (S) -2-t-butoxycarbonylamino-4- (3- (3- (4- (pyridin-2-yloxymethyl) -benzyl) -isoxazol-5-yl) described in Preparation Example 4-2 To a mixture of -pyridin-2-ylcarbamoyl) -butyric acid ethyl ester (40 mg, 0.065 mmol) and dichloromethane (1 mL) was added trifluoroacetic acid (0.5 mL) at room temperature, and the mixture was stirred overnight at the same temperature. The reaction mixture was evaporated under reduced pressure, and the obtained residue was washed with diethyl ether to give the title compound (30 mg) as a trifluoroacetate salt.
1 H-NMR Spectrum (DMSO-d 6 ) δ (ppm): 1.24 (3H, t, J = 7.1 Hz), 1.97-2.03 (2H, m), 2.41-2.56 (2H, m), 4.01-4.03 ( 3H, m), 4.21 (2H, q, J = 7.1Hz), 5.32 (2H, s), 6.66 (1H, s), 6.84-6.86 (1H, m), 6.98-7.01 (1H, m), 7.30 (2H, d, J = 8.2Hz), 7.41 (2H, d, J = 8.1Hz), 7.43 (1H, d, J = 7.7Hz), 7.70-7.75 (1H, m), 8.16-8.18 (2H, m), 8.38 (3H, brs), 8.52 (1H, dd, J = 1.8,4.8Hz), 10.48 (1H, s).
　出発物質（Ｓ）－２－ｔ－ブトキシカルボニルアミノ－４－（３－（３－（４－（ピリジン－２－イルオキシメチル）－ベンジル）－イソキサゾール―５－イル）－ピリジン―２－イルカルバモイル）－ブチリック　アシド　エチル　エステルは以下の方法で合成した。 Starting material (S) -2-t-butoxycarbonylamino-4- (3- (3- (4- (pyridin-2-yloxymethyl) -benzyl) -isoxazol-5-yl) -pyridin-2-y Rucarbamoyl) -butyric acid ethyl ester was synthesized by the following method.
［製造例４－１］（Ｓ）－２－ｔ－ブトキシカルボニルアミノ－ペンタンジオイック　アシド　５－ベンジル　エステル　１－エチル　エステル [Production Example 4-1] (S) -2-t-butoxycarbonylamino-pentanedioic acid 5-benzyl ester 1-ethyl ester
Figure JPOXMLDOC01-appb-C000091
 　（Ｓ）－２－ｔ－ブトキシカルボニルアミノ－ペンタンジオイック　アシド　５－ベンジル　エステル（３００ｍｇ、０．８９ｍｍｏｌ）とメタノール（４ｍＬ）の混合物に、０℃でＮ－メチルモルホリン（９８μＬ、０．８９ｍｍｏｌ）、ベンゾトリアゾール－１－イルオキシトリス－（ジメチルアミノ）－ホスホニウム　ヘキサフルオロホスフェート（３９０ｍｇ、０．８９ｍｍｏｌ）、及びアセトニトリル（２ｍＬ）を加え、室温で終夜攪拌した。反応混合物に１Ｎ水酸化ナトリウム水溶液を加え、酢酸エチルで抽出した。有機層を水及び飽和食塩水で順次洗浄し、無水硫酸マグネシウムで乾燥した。減圧下溶媒留去し、標記化合物（４１０ｍｇ、純度６３％）を粗体として得た。
1H－NMR  Spectrum  (CDCl3)δ(ppm)：　1.27(3H,t,J=7.1Hz),  1.44(9H,s),　1.91-2.01(1H,m),　2.18-2.21(1H,m),　2.39-2.52(2H,m),　4.16-4.22(2H,m),　4.32-4.33(1H,m), 5.10-5.12(3H,m),   7.31-7.39(5H,m).
Figure JPOXMLDOC01-appb-C000091
 To a mixture of (S) -2-t-butoxycarbonylamino-pentanedioic acid 5-benzyl ester (300 mg, 0.89 mmol) and methanol (4 mL) at 0 ° C., N-methylmorpholine (98 μL, 0.89 mmol). , Benzotriazol-1-yloxytris- (dimethylamino) -phosphonium hexafluorophosphate (390 mg, 0.89 mmol) and acetonitrile (2 mL) were added, and the mixture was stirred at room temperature overnight. To the reaction mixture was added 1N aqueous sodium hydroxide solution, and the mixture was extracted with ethyl acetate. The organic layer was washed successively with water and saturated brine, and dried over anhydrous magnesium sulfate. The solvent was distilled off under reduced pressure to obtain the title compound (410 mg, purity 63%) as a crude product.
1 H-NMR Spectrum (CDCl 3 ) δ (ppm): 1.27 (3H, t, J = 7.1Hz), 1.44 (9H, s), 1.91-2.01 (1H, m), 2.18-2.21 (1H, m) , 2.39-2.52 (2H, m), 4.16-4.22 (2H, m), 4.32-4.33 (1H, m), 5.10-5.12 (3H, m), 7.31-7.39 (5H, m).
［製造例４－２］（Ｓ）－２－ｔ－ブトキシカルボニルアミノ－４－（３－（３－（４－（ピリジン－２－イルオキシメチル）－ベンジル）－イソキサゾール―５－イル）－ピリジン―２－イルカルバモイル）－ブチリック　アシド　エチル　エステル [Production Example 4-2] (S) -2-t-butoxycarbonylamino-4- (3- (3- (4- (pyridin-2-yloxymethyl) -benzyl) -isoxazol-5-yl)- Pyridin-2-ylcarbamoyl) -butyric acid ethyl ester
Figure JPOXMLDOC01-appb-C000092
 　製造例４－１に記載の（Ｓ）－２－ｔ－ブトキシカルボニルアミノ－ペンタンジオイック　アシド　５－ベンジル　エステル　１－エチル　エステル（純度６３％、３９０ｍｇ、０．６７ｍｍｏｌ）とエタノール（４ｍＬ）の混合物に、室温でパラジウム－カーボン（５０％含水、４０ｍｇ、０．１９ｍｍｏｌ）を加え、水素雰囲気下（１ａｔｍ）、室温で終夜攪拌した。反応混合物を窒素で置換し、セライトを用いてろ過した。ろ液を減圧下溶媒留去し、（Ｓ）－２－ｔ－ブトキシカルボニルアミノ－ペンタンジオイック　アシド　１－エチル　エステル　（３３０ｍｇ）を粗体として得た。得られた粗体をそのまま次の反応に用いた。参考例１に記載の３－（３－（４－（ピリジン－２－イルオキシメチル）－ベンジル）－イソキサゾール－５－イル）－ピリジン－２－イルアミン（１５０ｍｇ、０．４２ｍｍｏｌ）とアセトニトリル（３ｍＬ）の混合物に、０℃で（Ｓ）－２－ｔ－ブトキシカルボニルアミノ－ペンタンジオイック　アシド　１－エチル　エステル　（粗体、２３０ｍｇ）、Ｎ－メチルモルホリン（４６μＬ、０．４２ｍｍｏｌ）、Ｏ－（７－アザベンゾトリアゾール－１－イル）－１、１、３、３－テトラメチルウロミウム　ヘキサフルオロホスフェート（１８０ｍｇ、０．４６ｍｍｏｌ）を順次加え、室温で終夜攪拌した。反応混合物に水を加え、酢酸エチルで抽出した。有機層を飽和食塩水で洗浄し、減圧下溶媒留去した。残渣をＮＨシリカゲルカラムクロマトグラフィー（酢酸エチル：ヘプタン＝４：１）で精製し、標記化合物（４７ｍｇ）を得た。
1H－NMR  Spectrum  (CDCl3)δ(ppm)：　1.27(3H,t,J=7.1Hz),  1.42(9H,s),　1.94-2.05(1H,m),　2.23-2.31(1H,m),　2.64-2.72(2H,m),　4.08(2H,s),  4.19(2H,q,J=7.1Hz),　4.33-4.34(1H,m), 5.33-5.36(3H,m),   6.33(1H,s),  6.79-6.81(1H,m),  6.87-6.90(1H,m),  7.18(1H,dd,J=4.9,7.8Hz),  7.30(2H,d,J=8.1Hz),　7.44(2H,d,J=8.2Hz),  7.56-7.61(1H,m),  7.92-7.95(1H,m),  8.17(1H,ddd,J=0.7,2.0,5.1Hz),　8.47(1H,dd,J=1.8,4.8Hz),　8.51(1H,brs).
Figure JPOXMLDOC01-appb-C000092
 A mixture of (S) -2-t-butoxycarbonylamino-pentanedioic acid 5-benzyl ester 1-ethyl ester (purity 63%, 390 mg, 0.67 mmol) and ethanol (4 mL) described in Preparation Example 4-1. To the mixture was added palladium-carbon (50% water content, 40 mg, 0.19 mmol) at room temperature, and the mixture was stirred overnight at room temperature under a hydrogen atmosphere (1 atm). The reaction mixture was purged with nitrogen and filtered through celite. The filtrate was evaporated under reduced pressure to give (S) -2-t-butoxycarbonylamino-pentanedioic acid 1-ethyl ester (330 mg) as a crude product. The obtained crude product was directly used in the next reaction. 3- (3- (4- (Pyridin-2-yloxymethyl) -benzyl) -isoxazol-5-yl) -pyridin-2-ylamine (150 mg, 0.42 mmol) and acetonitrile (3 mL) described in Reference Example 1 ) Was added at 0 ° C. to (S) -2-t-butoxycarbonylamino-pentanedioic acid 1-ethyl ester (crude, 230 mg), N-methylmorpholine (46 μL, 0.42 mmol), O- ( 7-Azabenzotriazol-1-yl) -1,1,3,3-tetramethyluronium hexafluorophosphate (180 mg, 0.46 mmol) was sequentially added, and the mixture was stirred at room temperature overnight. Water was added to the reaction mixture, and the mixture was extracted with ethyl acetate. The organic layer was washed with saturated brine, and the solvent was distilled off under reduced pressure. The residue was purified by NH silica gel column chromatography (ethyl acetate: heptane = 4: 1) to obtain the title compound (47 mg).
1 H-NMR Spectrum (CDCl 3 ) δ (ppm): 1.27 (3H, t, J = 7.1Hz), 1.42 (9H, s), 1.94-2.05 (1H, m), 2.23-2.31 (1H, m) , 2.64-2.72 (2H, m), 4.08 (2H, s), 4.19 (2H, q, J = 7.1Hz), 4.33-4.34 (1H, m), 5.33-5.36 (3H, m), 6.33 (1H , s), 6.79-6.81 (1H, m), 6.87-6.90 (1H, m), 7.18 (1H, dd, J = 4.9,7.8Hz), 7.30 (2H, d, J = 8.1Hz), 7.44 ( 2H, d, J = 8.2Hz), 7.56-7.61 (1H, m), 7.92-7.95 (1H, m), 8.17 (1H, ddd, J = 0.7,2.0,5.1Hz), 8.47 (1H, dd, J = 1.8,4.8Hz), 8.51 (1H, brs).
　式（Ｉ）で表される本発明化合物は、マウスのカンジダ全身感染実験により、顕著に平均生存日数が改善し、さらに、物性、特に水への溶解性及び水溶液中での安定性、並びに体内動態及び安全性の面でも優れており、真菌感染症の予防剤又は治療剤として極めて有用である。 The compound of the present invention represented by the formula (I) has a significantly improved average survival time according to a Candida systemic infection experiment in mice. Furthermore, the physical properties, particularly solubility in water and stability in aqueous solution, It is excellent in terms of kinetics and safety and is extremely useful as a preventive or therapeutic agent for fungal infections.
[水への溶解性の比較試験例]
　親化合物である参考例１に記載の３－（３－（４－（ピリジン－２－イルオキシメチル）－ベンジル）－イソキサゾール－５－イル）－ピリジン－２－イルアミンと実施例１ないし３の化合物を、２５℃において、Britton-Robinson緩衝液（イオン強度０．３）への溶解度と溶液中安定性を比較した。表１はその結果を示す。 [Comparative test of solubility in water]
3- (3- (4- (Pyridin-2-yloxymethyl) -benzyl) -isoxazol-5-yl) -pyridin-2-ylamine described in Reference Example 1, which is the parent compound, and Examples 1 to 3 The compounds were compared for solubility in solution and stability in solution at 25 ° C. in Britton-Robinson buffer (ionic strength 0.3). Table 1 shows the results.
Figure JPOXMLDOC01-appb-T000001
Figure JPOXMLDOC01-appb-T000001
　表１に示す結果から明らかなように、実施例１の化合物、実施例２の化合物、及び実施例３の化合物は、その親化合物よりも水への溶解性が増大していることが判明した。 As is clear from the results shown in Table 1, the compound of Example 1, the compound of Example 2, and the compound of Example 3 were found to have increased solubility in water as compared to the parent compound. .
[マウスにおける薬物動態評価]
１．実施例１の化合物のマウスにおける薬物動態評価
(1).投与液の調製
　実施例１の化合物は、10mM塩酸溶液（和光純薬工業）を含む5%グルコース（大塚製薬）にて0.3mg/mLに溶解し、親化合物である活性体は3mM塩酸溶液（和光純薬工業）を含む5%グルコース（大塚製薬）にて0.3mg/mLに溶解した。 [Pharmacokinetic evaluation in mice]
1. Pharmacokinetic evaluation of the compound of Example 1 in mice
(1). Preparation of Administration Solution The compound of Example 1 was dissolved in 0.3 mg / mL in 5% glucose (Otsuka Pharmaceutical) containing 10 mM hydrochloric acid solution (Wako Pure Chemical Industries), and the active substance as the parent compound was It was dissolved in 0.3 mg / mL with 5% glucose (Otsuka Pharmaceutical) containing 3 mM hydrochloric acid solution (Wako Pure Chemical Industries).
(2).投与、採血及び血漿採取
　5週齢の雌性ICR系マウス（日本チャールス・リバー）を使用し、実施例１の化合物は2匹を1群とし、活性体は3匹を１群とし、実施例１の化合物及び活性体を3mg/kgの投与量で尾静脈内へ投与した。実施例１の化合物は投与後30分、1、3、5、8時間に、活性体は投与後5分、15分、30分、1、2、4、6、8時間に尾静脈に穿刺し出血させ、ヘパリン処理したピペットで血液を採取した．採取した血液はサンプリングチューブに入れ氷冷下保存後、4℃、10，500xgで5分間遠心分離した。得られた血漿を正確に10μL採取し、分析時まで－20℃で保存した。 (2). Administration, Blood Collection and Plasma Collection Using a 5-week-old female ICR mouse (Nippon Charles River), the compound of Example 1 consists of 2 animals in 1 group and the active substance in 3 groups. The compound of Example 1 and the active form were administered into the tail vein at a dose of 3 mg / kg. The compound of Example 1 is punctured into the tail vein at 30 minutes, 1, 3, 5, 8 hours after administration, and the active substance is punctured at 5 minutes, 15 minutes, 30 minutes, 1, 2, 4, 6, 8 hours after administration. The blood was collected with a heparinized pipette. The collected blood was placed in a sampling tube and stored under ice cooling, and then centrifuged at 4 ° C. and 10,500 × g for 5 minutes. Accurate 10 μL of the obtained plasma was collected and stored at −20 ° C. until analysis.
(3).血漿中濃度測定法
　実施例１の化合物及び活性体の血漿中濃度は液体クロマトグラフ質量分析計（LC－MS/MS：Waters,  Quattro　Ultima　Pt）を用いて測定し、内部標準法にて定量した。イミプラミン塩酸塩（SIGMA）を、濃度が0.1μmol/Lになるようにアセトニトリル及びメタノール（1:1）混合溶液に溶解し、内部標準物質溶液（IS溶液）を調製した。血漿を融解後、氷上で冷却したままIS溶液を100μL加えて混合し、4℃、7800xgで10分間遠心分離（除蛋白）した後、上清をメンブレンフィルター（Millipore:　MultiScreenTM）にて遠心ろ過し、濾液をLC-MS/MS(Waters：Quattro  Ultima  Pt)にて分析した。得られたクロマトグラムにおいて、実施例１の化合物、活性体化合物（実施例１化合物の親化合物）及び内部標準物質に対応するピークの面積を、解析ソフトウェア(Waters：MassLynx　4.0)で解析し、内部標準法にて、血漿中に含まれる化合物の濃度を算出した。実施例１の化合物を投与した後の活性体濃度は、分子量比、すなわち（実施例１の化合物分子量）/（活性体分子量）の値で補正した。 (3). Plasma concentration measurement method The plasma concentration of the compound and active substance of Example 1 was measured using a liquid chromatograph mass spectrometer (LC-MS / MS: Waters, Quattro Ultima Pt), and the internal standard method was used. Was quantified. Imipramine hydrochloride (SIGMA) was dissolved in a mixed solution of acetonitrile and methanol (1: 1) to a concentration of 0.1 μmol / L to prepare an internal standard substance solution (IS solution). After thawing plasma, add 100 μL of IS solution while cooling on ice, mix, centrifuge (protein removal) at 7800 xg for 10 minutes at 4 ° C, and then centrifuge the supernatant with a membrane filter (Millipore: MultiScreen TM ) The filtrate was analyzed by LC-MS / MS (Waters: Quattro Ultima Pt). In the obtained chromatogram, the areas of peaks corresponding to the compound of Example 1, the active compound (parent compound of Example 1 compound) and the internal standard substance were analyzed with analysis software (Waters: MassLynx 4.0), and the internal The concentration of the compound contained in plasma was calculated by a standard method. The active substance concentration after administration of the compound of Example 1 was corrected by the molecular weight ratio, that is, the value of (Compound molecular weight of Example 1) / (Active substance molecular weight).
１．に記載の測定法で、実施例１の化合物及び活性体の血漿中濃度を算出した。その結果、図１に示す通り、実施例１の化合物の血漿中濃度は、投与後速やかに低下し、一方活性体の血漿中濃度は、実施例１の化合物投与後速やかに上昇し、活性体投与時と類似した血漿中濃度推移を示した。以上より、実施例１の化合物はマウス体内において速やかに活性体に変換することが示唆された。 1. The plasma concentrations of the compound of Example 1 and the active substance were calculated by the measurement method described in 1. above. As a result, as shown in FIG. 1, the plasma concentration of the compound of Example 1 decreases rapidly after administration, while the plasma concentration of the active substance increases rapidly after administration of the compound of Example 1, and the active substance The change in plasma concentration was similar to that at the time of administration. From the above, it was suggested that the compound of Example 1 was rapidly converted into an active form in the mouse body.
２．実施例２及び３の化合物のマウスにおける薬物動態評価
(1).投与液の調製
　実施例２及び３の化合物は、10mM塩酸溶液（和光純薬工業）を含む5%グルコース（大塚製薬）にてそれぞれ1、1.5mg/mLに溶解し、活性体は10mM塩酸溶液（和光純薬工業）を含む5%グルコース（大塚製薬）にて0.5mg/mLに溶解した。 2. Pharmacokinetic evaluation of the compounds of Examples 2 and 3 in mice
(1). Preparation of Dosing Solution The compounds of Examples 2 and 3 were dissolved in 1 and 1.5 mg / mL in 5% glucose (Otsuka Pharmaceutical) containing 10 mM hydrochloric acid solution (Wako Pure Chemical Industries), respectively. Was dissolved in 0.5 mg / mL with 5% glucose (Otsuka Pharmaceutical) containing 10 mM hydrochloric acid solution (Wako Pure Chemical Industries).
(2).投与、採血及び血漿採取
　7週齢の雌性ICR系マウス（日本チャールス・リバー）を使用し、2匹を1群として、本発明化合物及び活性体をそれぞれ活性体当量換算して3mg/kgの投与量で尾静脈内へ投与した。投与後20分、45分、1.5、3、5、8時間に尾静脈に穿刺し出血させ、ヘパリン処理したピペットで血液を採取した。採取した血液はサンプリングチューブに入れ氷冷下保存後、4℃、10，500xgで5分間遠心分離した。得られた血漿を正確に5μL採取し、分析時まで－20℃で保存した。 (2). Administration, Blood Collection and Plasma Collection Using 7-week-old female ICR mice (Nippon Charles River), 2 mice as 1 group, the compound of the present invention and the active substance are each converted to active equivalents of 3 mg It was administered into the tail vein at a dose of / kg. At 20 minutes, 45 minutes, 1.5, 3, 5, and 8 hours after administration, the tail vein was punctured and bled, and blood was collected with a heparinized pipette. The collected blood was placed in a sampling tube and stored under ice cooling, and then centrifuged at 4 ° C. and 10,500 × g for 5 minutes. Accurate 5 μL of the obtained plasma was collected and stored at −20 ° C. until analysis.
(3).血漿中濃度測定法
　実施例２、３の化合物及び活性体の血漿中濃度は液体クロマトグラフ質量分析計（LC－MS：Waters;ZQ  mass  detector）を用いて測定し、内部標準法にて定量した。イミプラミン塩酸塩（SIGMA）を、濃度が1μmol/Lになるようにアセトニトリルとメタノールとの混合溶液（9:1）に溶解し、内部標準物質溶液（IS溶液）を調製した。血漿を融解後、氷上で冷却したままIS溶液を50μL加えて混合し、4℃、1607xgで10分間遠心分離（除蛋白）し、上清をLC-MS(Waters：ZQ　mass　detector)にて分析した。得られたクロマトグラムにおいて、実施例２、３の化合物、活性体化合物及び内部標準物質に対応するピークの面積を、解析ソフトウェア(Waters：MassLynx　4.0)で解析し、内部標準法にて、血漿中に含まれる化合物の濃度を算出した。 (3). Method for measuring plasma concentration Plasma concentrations of the compounds and active substances of Examples 2 and 3 were measured using a liquid chromatograph mass spectrometer (LC-MS: Waters; ZQ mass detector), and an internal standard method was used. Was quantified. Imipramine hydrochloride (SIGMA) was dissolved in a mixed solution (9: 1) of acetonitrile and methanol so as to have a concentration of 1 μmol / L to prepare an internal standard substance solution (IS solution). After thawing plasma, add 50 μL of IS solution while cooling on ice, mix, centrifuge at 4 ° C and 1607 xg for 10 minutes (protein removal), and analyze supernatant with LC-MS (Waters: ZQ mass detector) did. In the obtained chromatogram, the areas of peaks corresponding to the compounds of Examples 2 and 3, the active compound, and the internal standard substance were analyzed with analysis software (Waters: MassLynx 4.0). The concentration of the compound contained in was calculated.
　２．に記載の測定法で、実施例２、３の化合物及び活性体の血漿中濃度を算出した。その結果、図２に示す通り、実施例２及び３の化合物は検出されなかった。一方、活性体の血漿中濃度は、実施例２及び３の化合物投与後速やかに上昇し、活性体投与時と類似した血漿中濃度推移を示した。以上より、実施例２及び３の化合物はマウス体内において速やかに活性体に変換することが示唆された。 2. The plasma concentrations of the compounds of Examples 2 and 3 and the active substance were calculated by the measurement method described in 1. As a result, as shown in FIG. 2, the compounds of Examples 2 and 3 were not detected. On the other hand, the plasma concentration of the active form rapidly increased after the administration of the compounds of Examples 2 and 3, and showed a plasma concentration transition similar to that at the time of active form administration. From the above, it was suggested that the compounds of Examples 2 and 3 were rapidly converted into active forms in the mouse body.
　本発明によれば、式（Ｉ）で表される本発明化合物は、１）真菌のＧＰＩ生合成阻害に基づいて細胞壁表層蛋白質の発現を阻害し、細胞壁assemblyを阻害するとともに真菌が細胞へ付着するのを阻害して、病原体が病原性を発揮できないようにすることより、感染症の発症、進展、持続に対して効果を示し、２）物性、特に、水への溶解性及び水溶液中での安定性、並びに体内動態及び安全性の面でも優れる真菌感染症の予防又は治療剤として極めて有用である。 According to the present invention, the compound of the present invention represented by the formula (I) 1) inhibits the expression of cell wall surface protein based on inhibition of fungal GPI biosynthesis, inhibits cell wall assembly, and attaches to cells. Inhibiting the pathogen and preventing pathogens from exhibiting pathogenicity, it has an effect on the onset, progression and persistence of infectious diseases. 2) Physical properties, especially solubility in water and in aqueous solution It is extremely useful as a preventive or therapeutic agent for fungal infections that are excellent in terms of stability, pharmacokinetics and safety.

Claims (18)

  1. 下式（I）で表される化合物又はその塩；
    Figure JPOXMLDOC01-appb-C000093
     　式中、
    　Ｒ1が、水素原子、ハロゲン原子、アミノ基、Ｒ11－ＮＨ－（Ｒ11が、Ｃ1-6アルキル基、ヒドロキシＣ1-6アルキル基、Ｃ1-6アルコキシＣ1-6アルキル基、又はＣ1-6アルコキシカルボニルＣ1-6アルキル基を意味する。）、Ｒ12－（ＣＯ）－ＮＨ－（Ｒ12が、Ｃ1-6アルキル基又はＣ1-6アルコキシＣ1-6アルキル基）、Ｃ1-6アルキル基、ヒドロキシＣ1-6アルキル基、シアノＣ1-6アルキル基、Ｃ1-6アルコキシ基、又はＣ1-6アルコキシＣ1-6アルキル基を意味し；
    　Ｒ2が式
    Figure JPOXMLDOC01-appb-C000094
     で表される基を意味し；
    　Ｘ及びＹの一方が、窒素原子を、他方が、窒素原子又は酸素原子を意味し；
    　環Ａが、ハロゲン原子若しくはＣ1-6アルキル基を１個若しくは２個有していてもよい、５若しくは６員のへテロアリール環又はベンゼン環を意味し；
    　Ｚが、単結合、メチレン基、エチレン基、酸素原子、硫黄原子、－ＣＨ2Ｏ－、－ＯＣＨ2－、－ＮＨ－、－ＣＨ2ＮＨ－、－ＮＨＣＨ2－、－ＣＨ2Ｓ－、又は－ＳＣＨ2－を意味し；
    　Ｒ3が、水素原子、ハロゲン原子、又は、それぞれ置換基群αから選ばれる置換基を１個若しくは２個有していてもよい、Ｃ1-6アルキル基、Ｃ3-8シクロアルキル基、Ｃ6-10アリール基、５若しくは６員へテロアリール基、又は５若しくは６員の非芳香族系へテロ環式基を意味し；
    　Ｒ4が、水素原子又はハロゲン原子を意味し；
    　Ｒが、水素原子、又はジメチルアミノ基で置換されていてもよいＣ1-6アルキル基を意味する。
    ［置換基群α］
    ハロゲン原子、シアノ基、Ｃ1-6アルキル基、Ｃ1-6アルコキシ基、Ｃ1-6アルコキシカルボニル基、Ｃ3-8シクロアルキル基、Ｃ2-6アルケニル基、及びＣ2-6アルキニル基。     A compound represented by the following formula (I) or a salt thereof;
    Figure JPOXMLDOC01-appb-C000093
     Where
    R 1 is a hydrogen atom, a halogen atom, an amino group, R 11 —NH— (R 11 is a C 1-6 alkyl group, a hydroxy C 1-6 alkyl group, a C 1-6 alkoxy C 1-6 alkyl group, Or a C 1-6 alkoxycarbonyl C 1-6 alkyl group), R 12 — (CO) —NH— (wherein R 12 represents a C 1-6 alkyl group or a C 1-6 alkoxy C 1-6 alkyl group). Group), a C 1-6 alkyl group, a hydroxy C 1-6 alkyl group, a cyano C 1-6 alkyl group, a C 1-6 alkoxy group, or a C 1-6 alkoxy C 1-6 alkyl group;
    R 2 is the formula
    Figure JPOXMLDOC01-appb-C000094
     Means a group represented by:
    One of X and Y means a nitrogen atom, and the other means a nitrogen atom or an oxygen atom;
    Ring A represents a 5- or 6-membered heteroaryl ring or benzene ring optionally having one or two halogen atoms or C 1-6 alkyl groups;
    Z is a single bond, a methylene group, an ethylene group, an oxygen atom, a sulfur atom, -CH 2 O -, - OCH 2 -, - NH -, - CH 2 NH -, - NHCH 2 -, - CH 2 S-, Or -SCH 2- ;
    R 3 may have a hydrogen atom, a halogen atom, or one or two substituents each selected from substituent group α, a C 1-6 alkyl group, a C 3-8 cycloalkyl group, Means a C 6-10 aryl group, a 5 or 6 membered heteroaryl group, or a 5 or 6 membered non-aromatic heterocyclic group;
    R 4 represents a hydrogen atom or a halogen atom;
    R represents a hydrogen atom or a C 1-6 alkyl group which may be substituted with a dimethylamino group.
    [Substituent group α]
    Halogen atom, cyano group, C 1-6 alkyl group, C 1-6 alkoxy group, C 1-6 alkoxycarbonyl group, C 3-8 cycloalkyl group, C 2-6 alkenyl group, and C 2-6 alkynyl group .
  2. Figure JPOXMLDOC01-appb-C000095
     で表される部分構造が、下記の群から選ばれる部分構造である請求項１に記載の化合物又はその塩。
    Figure JPOXMLDOC01-appb-C000096
    Figure JPOXMLDOC01-appb-C000095
     The compound or its salt of Claim 1 whose partial structure represented by these is a partial structure chosen from the following group.
    Figure JPOXMLDOC01-appb-C000096
  3. 　Ｘ及びＹの一方が窒素原子で、他方が酸素原子である請求項１に記載の化合物又はその塩。 The compound or a salt thereof according to claim 1, wherein one of X and Y is a nitrogen atom and the other is an oxygen atom.
  4. Figure JPOXMLDOC01-appb-C000097
     で表される部分構造が、下式（ＩＩＩ）
    Figure JPOXMLDOC01-appb-C000098
     で表される部分構造、又は下式（ＩＶ）
    Figure JPOXMLDOC01-appb-C000099
     で表される部分構造である請求項３に記載の化合物又はその塩。
    Figure JPOXMLDOC01-appb-C000097
     The partial structure represented by the following formula (III)
    Figure JPOXMLDOC01-appb-C000098
     Or a partial structure represented by the following formula (IV)
    Figure JPOXMLDOC01-appb-C000099
     The compound or its salt of Claim 3 which is a partial structure represented by these.
  5. 　Ｘ及びＹがともに窒素原子である請求項１に記載の化合物又はその塩。 The compound or salt thereof according to claim 1, wherein X and Y are both nitrogen atoms.
  6. Figure JPOXMLDOC01-appb-C000100
     で表される部分構造が、下式（Ｖ）
    Figure JPOXMLDOC01-appb-C000101
     で表される部分構造又は下式（ＶＩ）
    Figure JPOXMLDOC01-appb-C000102
     で表される部分構造である請求項５に記載の化合物又はその塩。
    Figure JPOXMLDOC01-appb-C000100
     The partial structure represented by the following formula (V)
    Figure JPOXMLDOC01-appb-C000101
     Or a partial structure represented by the following formula (VI)
    Figure JPOXMLDOC01-appb-C000102
     The compound or salt thereof according to claim 5, wherein the compound is a partial structure represented by the formula:
  7. 　Ｒが水素原子、メチル基、エチル基、又は２－ジメチルアミノエチル基である請求項１ないし６のいずれか１項に記載の化合物又はその塩。 7. The compound or a salt thereof according to any one of claims 1 to 6, wherein R is a hydrogen atom, a methyl group, an ethyl group, or a 2-dimethylaminoethyl group.
  8. 　Ｒ1が、水素原子、アミノ基、又はＣ1-6アルコキシＣ1-6アルキル基である請求項７に記載の化合物又はその塩。 The compound or a salt thereof according to claim 7, wherein R 1 is a hydrogen atom, an amino group, or a C 1-6 alkoxy C 1-6 alkyl group.
  9. 　Ｒ1がアミノ基であって、Ｒが水素原子、メチル基、エチル基、又は２－ジメチルアミノエチル基である請求項１ないし６のいずれか１項に記載の化合物又はその塩。 The compound or a salt thereof according to any one of claims 1 to 6, wherein R 1 is an amino group, and R is a hydrogen atom, a methyl group, an ethyl group, or a 2-dimethylaminoethyl group.
  10. 　Ｒ1がアミノ基であって、Ｒがメチル基、エチル基、又は２－ジメチルアミノエチル基である請求項１ないし６のいずれか１項に記載の化合物又はその塩。 The compound or a salt thereof according to any one of claims 1 to 6, wherein R 1 is an amino group, and R is a methyl group, an ethyl group, or a 2-dimethylaminoethyl group.
  11. 　環Ａが、ピリジン環、ベンゼン環、フラン環、チオフェン環、又はピロール環である請求項１ないし１０のいずれか１項に記載の化合物又はその塩。 The compound or a salt thereof according to any one of claims 1 to 10, wherein Ring A is a pyridine ring, a benzene ring, a furan ring, a thiophene ring, or a pyrrole ring.
  12. 　環Ａが、ピリジン環又はベンゼン環である請求項１１に記載の化合物又はその塩。 The ring or a salt thereof according to claim 11, wherein ring A is a pyridine ring or a benzene ring.
  13. 　Ｚが、酸素原子、－ＣＨ2Ｏ－、又は－ＯＣＨ2－である請求項１ないし１２のいずれか１項に記載の化合物又はその塩。 The compound or a salt thereof according to any one of claims 1 to 12, wherein Z is an oxygen atom, -CH 2 O-, or -OCH 2- .
  14. 　請求項１ないし１３のいずれか１項に記載の化合物又はその塩を含有する医薬組成物。 A pharmaceutical composition comprising the compound according to any one of claims 1 to 13 or a salt thereof.
  15. 　請求項１ないし１３のいずれか１項に記載の化合物又はその塩を含有する医薬。 A medicament comprising the compound or salt thereof according to any one of claims 1 to 13.
  16. 　請求項１ないし１３のいずれか１項に記載の化合物を有効成分とする抗真菌剤。 An antifungal agent comprising the compound according to any one of claims 1 to 13 as an active ingredient.
  17. 　請求項１ないし１３のいずれか１項に記載の化合物又はその塩の薬理学的有効量を投与して、真菌感染症を予防及び／又は治療する方法。 A method for preventing and / or treating a fungal infection by administering a pharmacologically effective amount of the compound or salt thereof according to any one of claims 1 to 13.
  18. 　抗真菌剤の製造のための請求項１ないし１３のいずれか１項に記載の化合物又はその塩の使用。 Use of the compound or a salt thereof according to any one of claims 1 to 13 for the manufacture of an antifungal agent.
PCT/JP2009/005559 2008-10-24 2009-10-22 PYRIDINE DERIVATIVE HAVING SUBSTITUTED HETERO RING AND SUBSTITUTED γ-GLUTAMYLAMINO GROUP, AND ANTI-FUNGAL AGENT COMPRISING SAME WO2010047120A1 (en)

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RU2011116160/04A RU2011116160A (en) 2008-10-24 2009-10-22 Pyridine derivatives substituted with a heterocyclic ring and y-glutamylamino group and containing antifungal agents
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EP09821820A EP2351752A4 (en) 2008-10-24 2009-10-22 PYRIDINE DERIVATIVE HAVING SUBSTITUTED HETERO RING AND SUBSTITUTED y-GLUTAMYLAMINO GROUP, AND ANTI-FUNGAL AGENT COMPRISING SAME
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BRPI0920614A BRPI0920614A2 (en) 2008-10-24 2009-10-22 heterocyclic ring-substituted pyridine derivatives and <sym> -glutamylamino group, and antifungal agents containing these derivatives
CA2740982A CA2740982A1 (en) 2008-10-24 2009-10-22 Pyridine derivatives substituted with heterocyclic ring and y-glutamylamino group, and antifungal agents containing same
MX2011003389A MX2011003389A (en) 2008-10-24 2009-10-22 Pyridine derivative having substituted hetero ring and substituted î³-glutamylamino group, and anti-fungal agent comprising same.
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IL211980A IL211980A0 (en) 2008-10-24 2011-03-28 Pyridine derivative having substituted hetero ring and substituted ??-glutamylamino group, and anti-fungal agent comprising same
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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011051198A2 (en) 2009-10-30 2011-05-05 Bayer Cropscience Ag Pyridine derivatives as agricultural pesticides
EP2345328A1 (en) * 2008-09-19 2011-07-20 Sumitomo Chemical Company, Limited Composition for agricultural use
JP2021530445A (en) * 2018-06-25 2021-11-11 アンプリックス ファーマシューティカルズ，インク． Pyridine derivatives substituted with heterocycles and amino groups

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005033079A1 (en) * 2003-09-30 2005-04-14 Eisai Co., Ltd. Novel antifungal agent comprising heterocyclic compound
TWI385169B (en) * 2005-10-31 2013-02-11 Eisai R&D Man Co Ltd Heterocyclic substituted pyridine derivatives and antifungal agent containing same
US8183264B2 (en) 2006-09-21 2012-05-22 Eisai R&D Managment Co., Ltd. Pyridine derivative substituted by heteroaryl ring, and antifungal agent comprising the same
TW200841879A (en) * 2007-04-27 2008-11-01 Eisai R&D Man Co Ltd Pyridine derivatives substituted by heterocyclic ring and phosphonoamino group, and anti-fungal agent containing same
US8513287B2 (en) * 2007-12-27 2013-08-20 Eisai R&D Management Co., Ltd. Heterocyclic ring and phosphonoxymethyl group substituted pyridine derivatives and antifungal agent containing same
US8188119B2 (en) * 2008-10-24 2012-05-29 Eisai R&D Management Co., Ltd Pyridine derivatives substituted with heterocyclic ring and γ-glutamylamino group, and antifungal agents containing same
JP2012176900A (en) * 2009-06-24 2012-09-13 Eisai R & D Management Co Ltd Pyridin derivative containing ((phosphonooxy)methyl) pyridinium ring and anti-fungal agent containing these derivative
JP6134478B2 (en) 2012-03-28 2017-05-24 ソニー株式会社 Information processing apparatus, display control method, program, and storage medium
EP3816155A1 (en) * 2013-08-23 2021-05-05 Virginia Commonwealth University Ester nitrates derivatives of aromatic aldehydes with multiple pharmalogic properties to treat sickle cell disease
CA3084298A1 (en) 2017-12-07 2019-06-13 Amplyx Pharmaceuticals, Inc. Heterocycle substituted pyridine derivative antifungal agents
CA3121202A1 (en) 2018-11-30 2020-06-04 Nuvation Bio Inc. Pyrrole and pyrazole compounds and methods of use thereof
JP7156154B2 (en) * 2019-04-18 2022-10-19 株式会社島津製作所 Medium processing system and medium processing method
CN113484308B (en) * 2021-06-25 2023-01-03 兰州大学 Test paper for rapidly identifying true and false wine and preparation and application thereof

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH05294935A (en) * 1991-03-15 1993-11-09 Green Cross Corp:The Aminopyridine-based compound
JPH08175993A (en) * 1994-12-21 1996-07-09 Green Cross Corp:The Antioxidizing agent
WO2002004626A1 (en) 2000-07-07 2002-01-17 Eisai Co., Ltd. Fungal cell wall synthesis gene
WO2005033079A1 (en) 2003-09-30 2005-04-14 Eisai Co., Ltd. Novel antifungal agent comprising heterocyclic compound
WO2007052615A1 (en) 2005-10-31 2007-05-10 Eisai R & D Management Co., Ltd. Pyridine derivative substituted by heterocycle and fungicide containing the same
US20070105904A1 (en) 2005-10-31 2007-05-10 Eisai R&D Management Co., Ltd. Heterocycles substituted pyridine derivatives and antifungal agent containing thereof
WO2008136279A1 (en) 2007-04-27 2008-11-13 Eisai R & D Management Co., Ltd. Salt of heterocycle-substituted pyridine derivative or crystal thereof

Family Cites Families (100)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB919073A (en) 1960-09-15 1963-02-20 Farmaceutici Italia Benzylamine derivatives
JPS542325A (en) 1977-06-07 1979-01-09 Sankyo Co Ltd Agricultural and horticultural pesticide
US4551532A (en) 1980-05-08 1985-11-05 Takeda Chemical Industries, Ltd. Ethylene glycol derivatives having anti-protozoan, anti-fungal and anti-tumor activity
JPS57179192A (en) 1981-04-27 1982-11-04 Takeda Chem Ind Ltd Ethylene glycol derivative
JPS5973575A (en) 1982-10-05 1984-04-25 Shionogi & Co Ltd Propynylaminoisoxazole derivative
JPS5984824A (en) 1982-11-08 1984-05-16 Takeda Chem Ind Ltd Antitumor agent
GB8311003D0 (en) 1983-04-22 1983-05-25 Shell Int Research Aniline compounds
DE3316187A1 (en) 1983-05-04 1984-11-08 Cassella Ag, 6000 Frankfurt 2-(Aminomethyl)pyrrole derivatives, process for their preparation, and their use
EP0124067B1 (en) 1983-05-04 1986-12-17 CASSELLA Aktiengesellschaft 2-(aminoalkyl)-pyrrole derivatives, process for their manufacture and their use
WO1986003203A1 (en) * 1984-11-22 1986-06-05 Yoshitomi Pharmaceutical Industries, Ltd. Thienylthiazole derivatives
JP2556849B2 (en) 1986-02-13 1996-11-27 三共株式会社 Glycerin derivative
US5070082A (en) * 1986-10-23 1991-12-03 American Cyanamid Company Solubilized pro-drugs
US4851423A (en) 1986-12-10 1989-07-25 Schering Corporation Pharmaceutically active compounds
JPS643162A (en) 1987-06-26 1989-01-06 Tokuyama Soda Co Ltd Sulfonamide compound
DE326326T1 (en) 1988-01-25 1990-02-08 Santen Pharmaceutical Co., Ltd, Osaka, Jp CYSTEIN DERIVATIVES.
IL89028A0 (en) * 1988-01-29 1989-08-15 Lilly Co Eli Quinoline,quinazoline and cinnoline derivatives
US5034393A (en) 1989-07-27 1991-07-23 Dowelanco Fungicidal use of pyridopyrimidine, pteridine, pyrimidopyrimidine, pyrimidopyridazine, and pyrimido-1,2,4-triazine derivatives
JPH03161470A (en) 1989-11-17 1991-07-11 Nisshin Flour Milling Co Ltd Indole derivative
US5371086A (en) * 1991-03-15 1994-12-06 The Green Cross Corporation Aminopyridine compounds
US5208247A (en) * 1991-08-01 1993-05-04 American Cyanamid Company Pyridinium compounds which are useful as antagonists of platelet activating factor
EP0533130A1 (en) 1991-09-19 1993-03-24 Hoechst Aktiengesellschaft 2-Hydroxymethylpyridines, the corresponding pyridine-N-oxides and derivatives thereof, process for their preparation and their use
ATE198773T1 (en) * 1991-10-23 2001-02-15 Baylor College Medicine FINGERPRINT IDENTIFICATION OF BACTERIAL STRAINS USING AMPLIFICATION OF REPETITIVE DNA SEQUENCES
SE9103776D0 (en) 1991-12-19 1991-12-19 Astra Ab NEW COMPOUNDS
EP0670831A1 (en) 1993-09-28 1995-09-13 Otsuka Pharmaceutical Co., Ltd. Quinoxaline derivative as antidiabetic agent
EP0738264A1 (en) 1994-01-07 1996-10-23 Dowelanco N-(4-pyrimidinyl)amide pesticides
TW385308B (en) * 1994-03-04 2000-03-21 Merck & Co Inc Prodrugs of morpholine tachykinin receptor antagonists
DE4434637A1 (en) * 1994-09-28 1996-04-04 Hoechst Schering Agrevo Gmbh Substituted pyridines, processes for their preparation and their use as pesticides and fungicides
US5747518A (en) * 1995-04-11 1998-05-05 Mitsui Toatsu Chemicals, Inc. Substituted thiophene derivative and agricultural and horticultural fungicide containing the same as active ingredient
US5710171A (en) * 1995-05-24 1998-01-20 Merck & Co., Inc. Bisphenyl inhibitors of farnesyl-protein transferase
US6080767A (en) * 1996-01-02 2000-06-27 Aventis Pharmaceuticals Products Inc. Substituted n-[(aminoiminomethyl or aminomethyl)phenyl]propyl amides
JP2000504336A (en) 1996-02-02 2000-04-11 ゼネカ・リミテッド Heterocyclic compounds useful as pharmaceutical preparations
GB9605437D0 (en) 1996-03-15 1996-05-15 Iaf Biochem Int Cytomegalovirus inhibiting compounds
US5945431A (en) * 1996-03-15 1999-08-31 Biochem Therapeutics Incorporated Cytomegalovirus inhibiting compounds
EP0796619A1 (en) * 1996-03-21 1997-09-24 Merrell Pharmaceuticals Inc. Use of (+)-alpha-(2,3-dimethoxyphenyl)-1-(2-(4-fluorophenyl)ethyl)-4-piperidinemethanol in treating depressive disorders and bipolar disorders
US6080757A (en) * 1996-06-06 2000-06-27 Pfizer Inc Antibiotic quinolones and derivatives
US6174905B1 (en) * 1996-09-30 2001-01-16 Mitsui Chemicals, Inc. Cell differentiation inducer
ID22781A (en) * 1997-04-04 1999-12-09 Pfizer Prod Inc Nicotynamide derivatives
US20020111495A1 (en) * 1997-04-04 2002-08-15 Pfizer Inc. Nicotinamide acids, amides, and their mimetics active as inhibitors of PDE4 isozymes
ATE221887T1 (en) * 1997-05-08 2002-08-15 Merck Sharp & Dohme SUBSTITUTED 1,2,4-TRIAZOLO(3,4,-A)PHTHALAZINE DERIVATIVES AS GABA-ALPHA 5 LIGANDS
DE19727117A1 (en) 1997-06-26 1999-01-07 Boehringer Ingelheim Pharma Phenylalkyl derivatives, pharmaceutical compositions containing them and processes for their preparation
DE19732033A1 (en) * 1997-07-25 1999-01-28 Bayer Ag Triazolinethione-phosphoric acid derivatives
WO1999014203A1 (en) * 1997-09-16 1999-03-25 Takeda Chemical Industries, Ltd. Nitrogenous fused-ring compounds, process for the preparation of the same, and drugs
US6022884A (en) * 1997-11-07 2000-02-08 Amgen Inc. Substituted pyridine compounds and methods of use
CA2314408A1 (en) * 1997-12-11 1999-06-17 Biochem Pharma Inc. Antiviral compounds
WO1999033846A2 (en) 1997-12-31 1999-07-08 The University Of Kansas Water soluble prodrugs of secondary and tertiary amine containing drugs and methods of making thereof
PE20000942A1 (en) 1998-07-31 2000-09-28 Lilly Co Eli DERIVATIVES OF AMIDE, CARBAMATE AND UREA
JP2000178243A (en) 1998-12-14 2000-06-27 Teijin Ltd Biphenylamidine derivative
US6235728B1 (en) * 1999-02-19 2001-05-22 Bristol-Myers Squibb Company Water-soluble prodrugs of azole compounds
CA2364652A1 (en) * 1999-02-22 2000-08-31 Benoit Bachand [1,8] naphthyridine derivatives having antiviral activity
US6319944B1 (en) 1999-05-10 2001-11-20 Merck & Co., Inc. Aryl amidines, compositions containing such compounds and methods of use
GB9919588D0 (en) * 1999-08-18 1999-10-20 Hoechst Schering Agrevo Gmbh Fungicidal compounds
TWI262185B (en) 1999-10-01 2006-09-21 Eisai Co Ltd Carboxylic acid derivatives having anti-hyperglycemia and anti-hyperlipemia action, and pharmaceutical composition containing the derivatives
ATE332130T1 (en) 1999-10-08 2006-07-15 Affinium Pharm Inc FAB-I INHIBITORS
WO2001036003A2 (en) 1999-11-15 2001-05-25 Drug Innovation & Design, Inc. Selective cellular targeting: multifunctional delivery vehicles
US6414013B1 (en) * 2000-06-19 2002-07-02 Pharmacia & Upjohn S.P.A. Thiophene compounds, process for preparing the same, and pharmaceutical compositions containing the same background of the invention
US6513676B2 (en) * 2000-06-21 2003-02-04 Martin L. Clemmons Universal cup holder with food tray accessories
US20040038239A1 (en) * 2000-07-07 2004-02-26 Kappei Tsukahara Fungal cell wall synthesis gene
DE60137435D1 (en) * 2000-09-15 2009-03-05 Anormed Inc CHEMOKIN RECEPTOR BINDING HETEROCYCLIC COMPOUNDS
US7074742B2 (en) * 2000-09-18 2006-07-11 E. I. Du Pont De Nemours And Company Pyridinyl amides and imides for use as fungicides
EP1217000A1 (en) 2000-12-23 2002-06-26 Aventis Pharma Deutschland GmbH Inhibitors of factor Xa and factor VIIa
ES2239203T3 (en) 2001-01-31 2005-09-16 Pfizer Products Inc. NICOTINAMIDE DERIVATIVES AND THEIR MIMETICS AS INHIBITORS OF ISOZIMAS PDE4.
JP2002275159A (en) 2001-03-16 2002-09-25 Japan Tobacco Inc Hcp inhibitor and compound having hcp inhibiting activity
DE60201740T2 (en) * 2001-04-05 2005-11-03 Torrent Pharmaceuticals Ltd., Ahmedabad Heterocyclic Compounds for Aging and Diabetes Related Vasculular Diseases
GB0109122D0 (en) * 2001-04-11 2001-05-30 Smithkline Beecham Spa Novel compounds
EP1275653A1 (en) 2001-07-10 2003-01-15 Bayer CropScience S.A. Oxazolopyridines and their use as fungicides
EP1275301A1 (en) 2001-07-10 2003-01-15 Bayer CropScience S.A. Trisubstituted heterocyclic compounds and their use as fungicides
JP2005509594A (en) 2001-07-13 2005-04-14 バーチャル ドラッグ ディヴェロップメント、インコーポレイティッド NAD synthetase inhibitors and uses thereof
US20040198773A1 (en) * 2001-09-26 2004-10-07 Barry Hart Substituted 3-pyridyl oxazoles as c17,20 lyase inhibitors
KR100437972B1 (en) * 2001-10-27 2004-07-02 한국과학기술연구원 Pyrrolidinone Derivatives, Their Preparation and Pharmaceutical Composition Comprising the Same
EP1440072A4 (en) 2001-10-30 2005-02-02 Conforma Therapeutic Corp Purine analogs having hsp90-inhibiting activity
BR0214455A (en) * 2001-11-27 2004-11-03 Hoffmann La Roche Benzothiazole Derivatives
CA2468159A1 (en) 2001-11-27 2003-06-05 Merck & Co., Inc. 4-aminoquinoline compounds
US20030191158A1 (en) * 2002-02-11 2003-10-09 Magee Thomas V. Nicotinamide derivatives and a tiotropium salt in combination for the treatment of diseases
CA2474192C (en) 2002-02-12 2011-06-21 Smithkline Beecham Corporation Nicotinamide derivates useful as p38 inhibitors
PL208689B1 (en) * 2002-03-08 2011-05-31 Basf Ag Prothioconazole and insecticide based fungicide mixture, method for fighting harmful fungi and fungicide
KR100951499B1 (en) * 2002-03-20 2010-04-07 브리스톨-마이어스 스큅 컴퍼니 Phosphate Prodrugs of Fluorooxindoles
EP1501808B1 (en) * 2002-04-26 2008-07-02 Eli Lilly And Company Tachykinin receptor antagonists
EP1369420A1 (en) 2002-06-06 2003-12-10 Aventis Pharma Deutschland GmbH Inhibitors of the GPib - vWF interaction
AU2003244181A1 (en) 2002-06-25 2004-01-06 Yamanouchi Pharmaceutical Co., Ltd. Phenoxypyridine derivative or salt thereof
EP1536790A2 (en) 2002-08-09 2005-06-08 AstraZeneca AB Oxadiazoles as modulators of metabotropic glutamate receptor-5
PA8578101A1 (en) * 2002-08-13 2004-05-07 Warner Lambert Co HETEROBIARILO DERIVATIVES AS METALOPROTEINASE IN MATRIX INHIBITORS
GB0222495D0 (en) 2002-09-27 2002-11-06 Glaxo Group Ltd Compounds
JP4537855B2 (en) 2002-10-09 2010-09-08 久光製薬株式会社 Novel pyrazole compounds having antifungal activity
KR20070065452A (en) 2002-11-22 2007-06-22 에자이 알앤드디 매니지먼트 가부시키가이샤 Methods of screening for compounds that inhibit the biosynthesis of gpi in malaria parasites
WO2004052280A2 (en) 2002-12-10 2004-06-24 Imclone Systems Incorporated Anti-angiogenic compounds and their use in cancer treatment
SI1569907T1 (en) * 2002-12-13 2016-06-30 Ym Biosciences Australia Pty Ltd Nicotinamide-based kinase inhibitors
TW200500360A (en) 2003-03-01 2005-01-01 Astrazeneca Ab Hydroxymethyl compounds
DE10315572A1 (en) * 2003-04-05 2004-10-14 Merck Patent Gmbh Substituted pyrazoles
JP4156460B2 (en) 2003-07-09 2008-09-24 Ｔｄｋ株式会社 Work pickup method and apparatus, and mounting machine
BRPI0412918A (en) * 2003-07-25 2006-09-26 Novartis Ag p38 kinase inhibitors
US20090227799A1 (en) 2004-08-09 2009-09-10 Kazutaka Nakamoto Novel Antimalarial Agent Containing Heterocyclic Compound
EP1782811A4 (en) 2004-08-09 2010-09-01 Eisai R&D Man Co Ltd Novel antimalaria agent containing heterocyclic compound
US7829585B2 (en) * 2005-03-30 2010-11-09 Eisai R&D Management Co., Ltd. Antifungal agent containing pyridine derivative
WO2007056215A2 (en) 2005-11-02 2007-05-18 Cytovia, Inc. N-aryl-thienopyrimidin-4-amines and the use thereof
US8183264B2 (en) * 2006-09-21 2012-05-22 Eisai R&D Managment Co., Ltd. Pyridine derivative substituted by heteroaryl ring, and antifungal agent comprising the same
TW200841879A (en) 2007-04-27 2008-11-01 Eisai R&D Man Co Ltd Pyridine derivatives substituted by heterocyclic ring and phosphonoamino group, and anti-fungal agent containing same
WO2009081970A1 (en) 2007-12-26 2009-07-02 Eisai R & D Management Co., Ltd. Process for production of heterocycle-substituted pyridine derivative
US8513287B2 (en) * 2007-12-27 2013-08-20 Eisai R&D Management Co., Ltd. Heterocyclic ring and phosphonoxymethyl group substituted pyridine derivatives and antifungal agent containing same
US8188119B2 (en) * 2008-10-24 2012-05-29 Eisai R&D Management Co., Ltd Pyridine derivatives substituted with heterocyclic ring and γ-glutamylamino group, and antifungal agents containing same
JP2012176900A (en) 2009-06-24 2012-09-13 Eisai R & D Management Co Ltd Pyridin derivative containing ((phosphonooxy)methyl) pyridinium ring and anti-fungal agent containing these derivative

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH05294935A (en) * 1991-03-15 1993-11-09 Green Cross Corp:The Aminopyridine-based compound
JPH08175993A (en) * 1994-12-21 1996-07-09 Green Cross Corp:The Antioxidizing agent
WO2002004626A1 (en) 2000-07-07 2002-01-17 Eisai Co., Ltd. Fungal cell wall synthesis gene
WO2005033079A1 (en) 2003-09-30 2005-04-14 Eisai Co., Ltd. Novel antifungal agent comprising heterocyclic compound
WO2007052615A1 (en) 2005-10-31 2007-05-10 Eisai R & D Management Co., Ltd. Pyridine derivative substituted by heterocycle and fungicide containing the same
US20070105904A1 (en) 2005-10-31 2007-05-10 Eisai R&D Management Co., Ltd. Heterocycles substituted pyridine derivatives and antifungal agent containing thereof
WO2008136279A1 (en) 2007-04-27 2008-11-13 Eisai R & D Management Co., Ltd. Salt of heterocycle-substituted pyridine derivative or crystal thereof

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
EXP. OPIN. THER. PATENTS, vol. 5, no. 9, 1995, pages 873 - 885
See also references of EP2351752A4

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2345328A1 (en) * 2008-09-19 2011-07-20 Sumitomo Chemical Company, Limited Composition for agricultural use
EP2345328A4 (en) * 2008-09-19 2014-06-25 Sumitomo Chemical Co Composition for agricultural use
WO2011051198A2 (en) 2009-10-30 2011-05-05 Bayer Cropscience Ag Pyridine derivatives as agricultural pesticides
JP2021530445A (en) * 2018-06-25 2021-11-11 アンプリックス ファーマシューティカルズ，インク． Pyridine derivatives substituted with heterocycles and amino groups

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