WO2009078307A1 - 液体フローに含まれる生物学的粒子を分別する装置ならびにその方法 - Google Patents

液体フローに含まれる生物学的粒子を分別する装置ならびにその方法 Download PDF

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WO2009078307A1
WO2009078307A1 PCT/JP2008/072308 JP2008072308W WO2009078307A1 WO 2009078307 A1 WO2009078307 A1 WO 2009078307A1 JP 2008072308 W JP2008072308 W JP 2008072308W WO 2009078307 A1 WO2009078307 A1 WO 2009078307A1
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biological particle
biological
liquid flow
particles
moving speed
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PCT/JP2008/072308
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English (en)
French (fr)
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Masahiko Kanda
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Bay Bioscience Kabushiki Kaisha
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Priority to US12/735,044 priority Critical patent/US8358412B2/en
Publication of WO2009078307A1 publication Critical patent/WO2009078307A1/ja

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/149—Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Zoology (AREA)
  • Virology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

　本願発明に係る液体フローに含まれる生物学的粒子を分別する装置は、前記生物学的粒子のそれぞれに光を照射して、該生物学的粒子からの光を検出する光学的機構と、前記生物学的粒子のそれぞれからの光に基づいて、前記液体フローにおける該生物学的粒子の移動速度を検出する制御部と、前記生物学的粒子のそれぞれの前記移動速度に基づいて、該生物学的粒子に電荷を与える荷電部とを備える。前記制御部は、ブレイク・オフ・ポイントで液体フローから分離する少なくとも１つの液滴フロー内における前記生物学的粒子のそれぞれの位置を、該生物学的粒子のそれぞれの前記移動速度に基づいてモニタし、該位置に基づいて該生物学的粒子に電荷を与えるように前記荷電部を制御する。本願発明によれば、所望の細胞粒子の回収率の増大、および分別試料中の細胞粒子の純度の改善を図ることができる。
PCT/JP2008/072308 2007-12-14 2008-12-09 液体フローに含まれる生物学的粒子を分別する装置ならびにその方法 WO2009078307A1 (ja)

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US12/735,044 US8358412B2 (en) 2007-12-14 2008-12-09 System and process for sorting biological particles in liquid flow

Applications Claiming Priority (2)

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JP2007323225A JP4990746B2 (ja) 2007-12-14 2007-12-14 液体フローに含まれる生物学的粒子を分別する装置ならびにその方法
JP2007-323225 2007-12-14

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WO2011112656A1 (en) * 2010-03-09 2011-09-15 Beckman Coulter, Inc. Calculate drop delay for flow cytometry systems and methods
CN103357583A (zh) * 2012-03-30 2013-10-23 索尼公司 微粒分选装置和延迟时间确定方法
CN104096687A (zh) * 2013-04-04 2014-10-15 索尼公司 微粒分选装置和微粒分选方法
JP2015507204A (ja) * 2012-02-09 2015-03-05 ベックマン コールター， インコーポレイテッド 選別フローサイトメータ
CN104755906A (zh) * 2012-11-08 2015-07-01 索尼公司 微粒分取装置和分取微粒的方法
JP2017201278A (ja) * 2016-05-06 2017-11-09 アライドフロー株式会社 生物学的粒子を含む液体フローを形成する装置および処理装置
JP2017211393A (ja) * 2017-08-02 2017-11-30 ソニー株式会社 粒子分取装置及び粒子分取方法
JP2019132853A (ja) * 2019-04-04 2019-08-08 ソニー株式会社 粒子分取装置及び粒子分取方法
JP2020190575A (ja) * 2020-08-25 2020-11-26 ソニー株式会社 粒子分取装置及び粒子分取方法
WO2022264481A1 (ja) * 2021-06-18 2022-12-22 ソニーグループ株式会社 粒子分取装置、粒子分取方法及びプログラム
WO2023007777A1 (ja) * 2021-07-26 2023-02-02 ソニーグループ株式会社 粒子分取装置及び粒子分取方法

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CN104096687A (zh) * 2013-04-04 2014-10-15 索尼公司 微粒分选装置和微粒分选方法
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JP2017201278A (ja) * 2016-05-06 2017-11-09 アライドフロー株式会社 生物学的粒子を含む液体フローを形成する装置および処理装置
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JP2020190575A (ja) * 2020-08-25 2020-11-26 ソニー株式会社 粒子分取装置及び粒子分取方法
JP2022001888A (ja) * 2020-08-25 2022-01-06 ソニーグループ株式会社 フローサイトメトリーシステム、及び粒子分析方法
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WO2023007777A1 (ja) * 2021-07-26 2023-02-02 ソニーグループ株式会社 粒子分取装置及び粒子分取方法

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JP4990746B2 (ja) 2012-08-01
US20100297759A1 (en) 2010-11-25
JP2009145213A (ja) 2009-07-02
US8358412B2 (en) 2013-01-22

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