WO2008149797A1 - 誘電体微粒子濃縮装置 - Google Patents

誘電体微粒子濃縮装置 Download PDF

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Publication number
WO2008149797A1
WO2008149797A1 PCT/JP2008/060001 JP2008060001W WO2008149797A1 WO 2008149797 A1 WO2008149797 A1 WO 2008149797A1 JP 2008060001 W JP2008060001 W JP 2008060001W WO 2008149797 A1 WO2008149797 A1 WO 2008149797A1
Authority
WO
WIPO (PCT)
Prior art keywords
microorganisms
captured
liquid sample
dielectrophoretic
dielectric microparticles
Prior art date
Application number
PCT/JP2008/060001
Other languages
English (en)
French (fr)
Inventor
Takaharu Enjoji
Satoshi Uchida
Original Assignee
Takaharu Enjoji
Satoshi Uchida
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Takaharu Enjoji, Satoshi Uchida filed Critical Takaharu Enjoji
Priority to US12/602,555 priority Critical patent/US20100193361A1/en
Priority to CN200880018365A priority patent/CN101730843A/zh
Priority to JP2009517837A priority patent/JP4548742B2/ja
Publication of WO2008149797A1 publication Critical patent/WO2008149797A1/ja

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C5/00—Separating dispersed particles from liquids by electrostatic effect
    • B03C5/02—Separators
    • B03C5/022—Non-uniform field separators
    • B03C5/026—Non-uniform field separators using open-gradient differential dielectric separation, i.e. using electrodes of special shapes for non-uniform field creation, e.g. Fluid Integrated Circuit [FIC]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N2001/4038—Concentrating samples electric methods, e.g. electromigration, electrophoresis, ionisation

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Sustainable Development (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Microelectronics & Electronic Packaging (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Electrostatic Separation (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

　試料液中の微生物（誘電体微粒子）を誘電泳動力によって捕集し、捕集された微生物の定量測定・分析後に、微生物を濃縮して回収する。 　誘電体微粒子濃縮装置１は、検査対象となる微生物を含む試料液を保持する試料液保持部１０から供給された試料液が印加されたセル１１を通過する際に、誘電泳動電極１１ａ～１１ｃ上に誘電泳動力によって微生物が捕集される。この捕集された微生物を、印加を停止して誘電泳動力から開放し、かつ、リリース液保持部１２から供給されるリリース液を誘電泳動電極１１ａ～１１ｃに貫流することで、放出させて、濃縮された微生物を標的菌として回収部１２に回収する。
PCT/JP2008/060001 2007-06-01 2008-05-30 誘電体微粒子濃縮装置 WO2008149797A1 (ja)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US12/602,555 US20100193361A1 (en) 2007-06-01 2008-05-30 Apparatus for concentrating dielectric microparticles
CN200880018365A CN101730843A (zh) 2007-06-01 2008-05-30 电介质微粒浓缩装置
JP2009517837A JP4548742B2 (ja) 2007-06-01 2008-05-30 誘電体微粒子濃縮装置

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007147503 2007-06-01
JP2007-147503 2007-06-01

Publications (1)

Publication Number Publication Date
WO2008149797A1 true WO2008149797A1 (ja) 2008-12-11

Family

ID=40093615

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/060001 WO2008149797A1 (ja) 2007-06-01 2008-05-30 誘電体微粒子濃縮装置

Country Status (4)

Country Link
US (1) US20100193361A1 (ja)
JP (1) JP4548742B2 (ja)
CN (1) CN101730843A (ja)
WO (1) WO2008149797A1 (ja)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011062131A (ja) * 2009-09-17 2011-03-31 Panasonic Corp 微生物濃度調整装置
JP2016059906A (ja) * 2014-09-22 2016-04-25 学校法人立命館 粒子の分離方法及び装置、並びにプロトプラストの捕捉方法
JP2018059946A (ja) * 2010-11-10 2018-04-12 ロシュ・ダイアグノスティクス・ヘマトロジー・インコーポレイテッドＲｏｃｈｅ Ｄｉａｇｎｏｓｔｉｃｓ Ｈｅｍａｔｏｌｏｇｙ， Ｉｎｃ． 検査用に生物試料を準備するための自動化されたシステムおよび方法

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101912732B (zh) * 2010-08-09 2012-02-01 天津富金环境技术研究有限公司 介电电泳法无污染回收贵重金属的高效连续工艺
US10274492B2 (en) 2015-04-10 2019-04-30 The Curators Of The University Of Missouri High sensitivity impedance sensor
WO2017061496A1 (ja) * 2015-10-07 2017-04-13 株式会社Ａｆｉテクノロジー 検査装置、検査システム、及び検査方法
CN113233556A (zh) * 2021-05-20 2021-08-10 大连海事大学 一种船舶压载水微生物浓缩装置及方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63177064A (ja) * 1987-01-19 1988-07-21 Toshiba Corp 自動化学分析装置
WO2004022774A1 (ja) * 2002-09-05 2004-03-18 Fuji Electric Systems Co.,Ltd. 微生物または細胞の検出方法
JP2006217808A (ja) * 2005-02-08 2006-08-24 Seiko Instruments Inc 観察用基板及び液滴供給装置
WO2007046485A1 (ja) * 2005-10-19 2007-04-26 Sharp Kabushiki Kaisha 誘電泳動チップおよび誘電泳動装置並びに誘電泳動システム

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002323416A (ja) * 2001-02-20 2002-11-08 Hitachi Ltd 検査容器
JP4254560B2 (ja) * 2004-01-30 2009-04-15 株式会社島津製作所 光学測定装置
JP4679197B2 (ja) * 2005-03-25 2011-04-27 株式会社東芝 微生物分離装置

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63177064A (ja) * 1987-01-19 1988-07-21 Toshiba Corp 自動化学分析装置
WO2004022774A1 (ja) * 2002-09-05 2004-03-18 Fuji Electric Systems Co.,Ltd. 微生物または細胞の検出方法
JP2006217808A (ja) * 2005-02-08 2006-08-24 Seiko Instruments Inc 観察用基板及び液滴供給装置
WO2007046485A1 (ja) * 2005-10-19 2007-04-26 Sharp Kabushiki Kaisha 誘電泳動チップおよび誘電泳動装置並びに誘電泳動システム

Non-Patent Citations (2)

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NITZAN GADSIH AND VOLDMAN J.: "High-Throughput Positive-Dielectrophoretic Bioparticle Microconcentrator", ANAL. CHEM., vol. 78, no. 22, 2006, pages 7870 - 7876 *
ZHOU GUANGBIN ET AL.: "Yuden Eido Filter o Mochiita Ekitaichu no Kendaku Biryushi no Jokyo to Kaishu", PROCEEDINGS OF THE INSTITUTE OF ELECTROSTATICS JAPAN, vol. 27, no. 1, 2003, pages 26 - 31 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011062131A (ja) * 2009-09-17 2011-03-31 Panasonic Corp 微生物濃度調整装置
JP2018059946A (ja) * 2010-11-10 2018-04-12 ロシュ・ダイアグノスティクス・ヘマトロジー・インコーポレイテッドＲｏｃｈｅ Ｄｉａｇｎｏｓｔｉｃｓ Ｈｅｍａｔｏｌｏｇｙ， Ｉｎｃ． 検査用に生物試料を準備するための自動化されたシステムおよび方法
US10175153B2 (en) 2010-11-10 2019-01-08 Roche Diagnostics Hematology, Inc. Automated systems and methods for preparing biological specimens for examination
US10775282B2 (en) 2010-11-10 2020-09-15 Roche Diagnostics Hematology, Inc Automated systems and methods for preparing biological specimens for examination
JP2016059906A (ja) * 2014-09-22 2016-04-25 学校法人立命館 粒子の分離方法及び装置、並びにプロトプラストの捕捉方法

Also Published As

Publication number Publication date
US20100193361A1 (en) 2010-08-05
CN101730843A (zh) 2010-06-09
JP4548742B2 (ja) 2010-09-22
JPWO2008149797A1 (ja) 2010-08-26

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