WO2008092297A1 - Nouveau procédé de préparation d'acide abscissique naturel - Google Patents

Nouveau procédé de préparation d'acide abscissique naturel Download PDF

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WO2008092297A1
WO2008092297A1 PCT/CN2007/000268 CN2007000268W WO2008092297A1 WO 2008092297 A1 WO2008092297 A1 WO 2008092297A1 CN 2007000268 W CN2007000268 W CN 2007000268W WO 2008092297 A1 WO2008092297 A1 WO 2008092297A1
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Prior art keywords
fermentation
abscisic acid
stage
inositol
hours
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PCT/CN2007/000268
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English (en)
Chinese (zh)
Inventor
Hong Tan
Baoliang Lei
Zhidong Li
Jinyan Zhou
Jie Yang
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Chengdu Institute Of Biology, The Chinese Academy Of Sciences
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Priority to PCT/CN2007/000268 priority Critical patent/WO2008092297A1/fr
Publication of WO2008092297A1 publication Critical patent/WO2008092297A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/145—Fungal isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi

Definitions

  • the present invention relates to a method for preparing natural abscisic acid by adding a solution of inositol to glucose repression during fermentation, and a novel strain used in the method, wherein the strain is subjected to gene-directed mutagenesis Obtained by improving the natural abscisic acid producing strains.
  • BACKGROUND OF THE INVENTION Abscisic acid (ABA) is one of the five major plant hormones that have been discovered in the world.
  • the natural type of abscisic acid has strong regulatory activity on the growth and development of crops, can promote the ripening and development of fruits, cereals and beans, can greatly improve its yield and quality, and greatly enhance its cold tolerance and drought resistance. With the ability to resist salt and alkali, it has broad application prospects.
  • CN1182798A discloses a method for producing natural abscisic acid by fungal fermentation, which adopts tertiary fermentation and is employed in tertiary fermentation. 2 ⁇ The continuous flow of feed and discharge and the immobilization of the strain, and the addition of key substrates, so that the acid production is stable, the highest yield of abscisic acid reached 1. 2 g / liter of fermentation broth. Patent ZL001.32024.
  • the present inventors conducted intensive research on the deficiencies of the glucose repression phenomenon, low fermentation yield, and high production cost in the process of producing natural abscisic acid by microbial fermentation, which has been known in the prior art.
  • the present invention has been completed by an improved process which is simpler and easier, has a higher yield of abscisic acid, and has a lower production cost. It is an object of the present invention to provide a method for fermentative production of natural abscisic acid by a fermentation process in which inositol is added to relieve glucose repression. Another object of the present invention is to provide a novel strain for producing natural abscisic acid.
  • the present invention provides a novel method for preparing a natural abscisic acid comprising the steps of: cultivating a fungus capable of producing a natural abscisic acid in a primary liquid medium (for example, medium A described below)
  • the fungus is selected from the group consisting of: Botrytis (for example, Botrytis cinerea) ⁇ > Cercospora (for example, Cercospora rosicola), Penicillium (Penici lliura), Aspergillus (Aspergillus) And a genetically modified strain of the above strain; and the first-stage seed liquid cultured in the above-mentioned first-stage liquid medium is inoculated into a second-stage liquid medium (for example, the medium B described below); The second-stage seed liquid cultured in the above second liquid medium is inoculated into a third-stage liquid medium (for example, the medium C described below) for a suitable period of time, and then the third-stage liquid is started.
  • Botrytis for
  • the medium is fed with fed fermentation and a certain concentration of inositol is added to relieve glucose repression and increase the utilization rate of other sugars in the third-stage liquid medium; ABA said fermentation broth was collected.
  • a specific embodiment of the present invention is the use of Botrytis cinerea (for example, Botrytis cinerea), Cercospora (for example, Cercospora rosicola), Aspergi llus or other fungi producing natural abscisic acid which produce natural abscisic acid and
  • Botrytis cinerea for example, Botrytis cinerea
  • Cercospora for example, Cercospora rosicola
  • Aspergi llus or other fungi producing natural abscisic acid which produce natural abscisic acid
  • the genetically modified bacteria are subjected to tertiary fermentation culture on a liquid medium, and different culture media are used in each fermentation stage.
  • a liquid medium suitable for the third-stage fermentation such as the medium C described below, is used as the fermentation medium, and the culture is carried out at a suitable temperature for a period of time, for example, at about 26 ° C. After 12-72 hours of incubation, the liquid medium is fed and fed, and a certain concentration of inositol is added at the same time.
  • the third-stage liquid medium (for example, medium C) can be fed continuously (continuous or non-uniform) and/or intermittent, and intermittent addition is preferred.
  • the method is known. Patent It is disclosed in ZL00132024.
  • the inositol addition method can adopt continuous (uniform or non-uniform) flow addition and/or intermittent flow addition mode, and continuous (constant speed or non-hook speed) flow addition mode is preferable.
  • the continuous (constant or non-uniform) flow addition method is to add a certain concentration of inositol and a continuous flow to the third-stage fermenter at a certain flow rate (hook speed or non-uniform speed) until the fermentation is stopped (the lower tank) ) For example, about 10 hours before.
  • the intermittent flow adding method is to intermittently add a certain concentration of inositol to the third-stage fermenting tank in a manner of adding a batch at intervals.
  • the intermittent time is preferably 1 to 5 times every 8 to 30 hours, more preferably about 1 hour at intervals of 10 hours.
  • Fermentation conditions temperature 23 ° C - 29 ° C, pH: 4-7
  • Fermentation time 8-9 days.
  • the abscisic acid in the fermentation broth is recovered in a conventional manner, for example, by extraction with an organic solvent extraction method, an ion exchange resin method, a macroporous adsorption resin method, a silica gel column chromatography method, and an activated carbon adsorption method, thereby obtaining a white crystalline abscisic acid.
  • the invention also employs a technical scheme such as a new strain to further increase the yield of natural fall acid.
  • the method of the present invention may employ known strains which produce natural abscisic acid, for example, Botrytis cinerea which is known, or obtained by genetic engineering or genetic engineering methods, which produces natural abscisic acid, for example, Botrytis cinerea, Cercospora (for example, Cercospora rosicola), Aspergillus (Aspergi llus), or other fungi that produce natural abscisic acid and genetically modified bacteria thereof.
  • known high-yielding strains which produce abscisic acid are used, such as Botrytis cinerea TB-3-H8, TBI-9 (CGMCC No. 0500, which is disclosed in the patent ZL-00132024.
  • the new strain is used in the most preferred method of the invention: the abscisic acid high-yield strain Botrytis cinerea TBC 10, which was deposited on December 15, 2006 at the General Microbiology Center of the China Collection of Microorganisms Collections (CGMCC) ), the accession number is CGMCC No. 1889, which uses the gene-directed mutagenesis technique of Botrytis cinerea TB-3-A3 to obtain a change of 24 bases and leads to the corresponding coded five.
  • CGMCC General Microbiology Center of the China Collection of Microorganisms Collections
  • the new strain TBC-10 (the sequence of the gene sequence and amino acid sequence is from the strain Botrytis cinerea 3. 3790, purchased by the Institute of Microbiology, Chinese Academy of Sciences, or other homology on Genebank) High species of FPPS).
  • the gene sequence of the TBC-10 strain has the following characteristics: The strain is aligned with the gene sequence of the strain Farethyl diphosphate/pyphosphate synthase (Bosstis cinerea) 3. 3790, There are 2 base changes.
  • the amino acid sequence of the TBC-10 strain has the following characteristics: The amino acid sequence alignment of the strain with the strain Farithyl diphosphate/pyphosphate synthase of Botrytis cinerea 3. 3790, There are five amino acid changes (1 to 5 sites); amino acid sequence alignments with farnesyl diphosphate/pyphosphate synthase (FPPS) from other highly homologous species on Genebank, There are four amino acid changes (1, 3, 4, 5 sites).
  • the process of the present invention employs a tertiary fermentation technique, and the use of tertiary fermentation to prepare abscisic acid is known (see, for example, CN1182798A).
  • the culture medium may be a medium suitable for the purpose known to those skilled in the art or the culture disclosed in the invention patent ZL00132024. base.
  • the use of the medium disclosed in the invention patent ZL00132024. 6 is preferred.
  • the invention discloses a medium A, B, and C, and the specific composition thereof is as follows: glucose ⁇ 0. 2%-3% beef extract 0. 1%-1% wheat bran 1%-5 sucrose 1% ⁇ 4 % ammonium nitrate 0. 1%-2% magnesium sulfate 0. 01%-0. 1% maltose 0. 1%-3% medium B starch 1%-5% wheat bran 1%-5% glucose 1%-4 %% maltose 0. 1%-3% Bean cake powder 0. 1%-2% ⁇ 0. 1%-5% Medium c
  • a preferred embodiment of the invention is based on the known fermentation process (disclosed in the patent ZL00132024. 6) in which a certain concentration of inositol is added. That is: the activated strain is inoculated into the medium A, and cultured in a solid culture or a flask at 23 ° C - 29 ° C for 24 to 76 hours, then inoculated with 10% - 50% of the inoculum. Fermentation in a seed tank with sterile medium B at 23 C-29 °C The culture is carried out for 24 to 60 hours, and then inoculated into a third-stage fermentor at a seeding rate of preferably 10% to 40% for fermentation production.
  • the third-stage fermentation uses medium C as the fermentation medium, and after fermentation for 12-72 hours at about 26 ° C, the feed of medium C and a certain concentration of inositol is started to be fed.
  • inositol There are two ways of adding inositol: one is continuous (uniform or non-uniform) flow, and the other is intermittent flow, the former is preferred.
  • inositol was continuously fed at a rate of 0.01 to 10 g/L * h (either at a constant rate or at a non-uniform rate) until about 10 hours before the fermentation was stopped (down can).
  • inositol is added intermittently, and the intermittent time is 1 to 5 times every 8-30 hours, preferably about 1 hour at intervals of 10 hours, and the amount of supplement is 0. 05 - 50 mg / L fermentation broth, preferably 0. 1 - 10 m g / L fermentation broth.
  • the bottom stream is fed without feeding.
  • the utilization rate of sugars such as sucrose, molasses, lactose, dextrin and other starch hydrolysates increases the acid production rate and reduces the production cost.
  • the fermentation broth is extracted by an organic solvent extraction method, an ion exchange resin method, a macroporous adsorption resin method, a silica gel column chromatography method, and an activated carbon adsorption method to obtain a white crystal abscisic acid.
  • Fermentation conditions Temperature: 23 ° C - 29. C, pH: 4-7 Fermentation time: 8-9 days.
  • the full process flow of the present invention is shown in Figure 1.
  • the production of the product is promoted by using a strain which produces a natural abscisic acid high-yield, and the production of abscisic acid is greatly improved.
  • Fermentation of natural abscisic acid by the method of the invention can artificially regulate the ratio of carbon and nitrogen in the fermentation system, relieve glucose repression, increase the utilization rate of other sugars in the culture medium, and make the strain have a higher substrate conversion rate.
  • FIG. 1 is a process flow diagram of the present invention.
  • DETAILED DESCRIPTION OF THE INVENTION The method of the present invention is further illustrated by the following non-limiting examples.
  • Example 1 25 pieces of 1000 ml flasks were sterilized by dissolving 300 ml of medium A in each bottle at 120 Torr. After cooling, the activated oxalic acid high-yield strain Botrytis cinerea TBC-10 CGMCC No. 1889 spore suspension was inoculated.
  • the cultured first-class seed solution is inoculated into a 100-liter fermenter (secondary seed tank) containing 50 liters of medium B at a dose of 13%-15%, and aeration is stirred at a temperature of 26 ° C to 28 ° C. Cultivate for 24-40 hours.
  • a 1 ton fermenter to make a three-stage tank fermentation.
  • the tank contains about 500 L of medium C.
  • the seed tank of the second tank is inoculated with 10%-15% inoculum. 1 % - 0. 5 % ⁇
  • the amount of the total volume of the fermentation broth is 0. 1% - 0. 5 % . 5-3. 0 mg/L fermentation broth.
  • the infusion of the inositol was intermittently added at intervals of 2 hours.
  • the combination of media used in the implementation process is as follows:
  • the fermentation temperature was 27 ° C
  • the fermentation pH was 4-7
  • the fermentation time reached 9 days.
  • the can fermentation broth is recovered by ion exchange resin method, eluted with ethanol, and recrystallized to obtain a white crystalline abscisic acid product.
  • the fermentation system was subjected to stable fermentation for 9 days, the contamination rate of the bacteria was 5.0%, and the product yield was 2.1 g of abscisic acid/liter of fermentation broth. After ion exchange and recrystallization recovery, the product recovery rate is 85%.
  • Example 2 50 pieces of 1000 ml triangular flasks were used, and each bottle of 300 ral medium A was sterilized at 12 CTC, and after cooling, the activated abscisic acid high-yield strain Botrytis cinerea TBC-10 CGMCC No. 1889 spore suspension was placed and placed. Shake the flask for 24 to 33 hours at 25 ° ⁇ .
  • the cultured first-class seed liquid is inoculated into a 200-liter fermenter (secondary seed tank) containing 100 liters of medium B according to the inoculation amount of 15%-18%, and agitated and cultured at a temperature of about 26 ° C. 30 hours. Use a 1 ton fermenter to make a three-stage tank fermentation.
  • the tank contains a medium C of about 500 L.
  • the second-stage tank seed solution is inoculated with 20% - 25% inoculum. 05%-0.
  • the total volume of the fermentation broth is 0. 05%-0. 3%.
  • Inositol was added continuously (either at a constant rate or at a constant rate) at a rate of 0.1 to 1.0 g/L ⁇ h until about 10 hours before the fermentation was stopped (down can).
  • the fermentation temperature was about 27 ° C, the fermentation pH was 4-7, and the fermentation time was 9 days.
  • the can fermentation broth is recovered by ion exchange resin method, eluted with ethanol, and recrystallized to obtain a white crystalline abscisic acid product.
  • the fermentation system was subjected to stable fermentation for 9 days, the contamination rate of the bacteria was 5.0%, and the product yield was 2.4 g of abscisic acid/liter of fermentation broth. After ion exchange and recrystallization recovery, the product recovery rate is 85%.
  • Example 3 30 bottles of 1000 ml flasks were used, and 300 ml of medium A per bottle was sterilized at 120 ° C.
  • the activated abscisic acid high-yield strain B. cinerea TBI-9 CGMCC No. 0500 spore suspension was inoculated. Shake the flask for 24 to 33 hours at 27 °C.
  • the cultured first-class seed liquid is inoculated into a 100-liter fermenter (second-stage seed tank) containing 50 liters of medium B according to the inoculation amount of 15%-20%, and the mixture is ventilated at a temperature of about 26 ° C. 30 hours.
  • the tank contains about 500 L of medium C.
  • the seed tank of the second tank is inoculated with 10%-15% inoculum.
  • the medium C was continuously fed at a rate of 0.1 to 5 L/h until 10 hours before the fermentation (lower tank) was stopped. Inositol was added continuously (either at a constant rate or at a constant rate) at a rate of 0.1 to 5. 0 yg/L, h until about 10 hours before the fermentation was stopped (down can).
  • the combination of media used in the implementation process is as follows: Glucose 3.0% Beef Cream 0.5% Wheat Bran 5.0% Sucrose 4.0%
  • the fermentation temperature was about 26 ° C, the fermentation pH was 4-7, and the fermentation time was 9 days.
  • the can fermentation broth is recovered by ion exchange resin method, eluted with ethanol, and recrystallized to obtain a white crystalline abscisic acid product.
  • the fermentation system was subjected to stable fermentation for 9 days, the contamination rate of the bacteria was 8. 0%, and the product yield was 2.1 g of abscisic acid/liter of fermentation broth. After ion exchange and recrystallization recovery, the product recovery rate is 84%.
  • Example 4 25 pieces of 1000 ml flasks were used, and 300 ml of medium A per bottle was sterilized at 120 ° C.
  • the activated abscisic acid high-yield strain TB-3 - H8 spore suspension was inoculated and placed at about 26 ° C. Shake the flask for 40 - 56 hours at temperature.
  • the cultured first-class seed solution is 15%-20°/.
  • the inoculation amount was inoculated into a 100-liter fermenter (secondary seed tank) containing 50 liters of medium B, and agitated and cultured at a temperature of 26 ° C - 27 ° C for 24-26 hours.
  • the seed tank of the second tank is inoculated with 10%-15% inoculum. 1%— 0. 0. 0. 1%— 0.
  • the total volume of the fermentation broth is 0.1% - 0. 5%.
  • Inositol was added continuously (either at a constant rate or at a constant rate) at a rate of 0.1 to 3. 0 ⁇ g/L ⁇ h until about 10 hours before the fermentation was stopped (down can).
  • the composition of the medium used in the process is as follows: glucose 0. 2%-3% beef extract 0. wheat bran 1 - 5 % sucrose 1% - 4% ammonium nitrate 0. 1% - 2% magnesium sulfate 0. 01% - 0 1%-% ⁇ 0.
  • the can fermentation broth is recovered by ion exchange resin method, eluted with ethanol, and recrystallized to obtain a white crystalline abscisic acid product.
  • the fermentation system was subjected to stable fermentation for 9 days, the contamination rate of the bacteria was 8. 0%, and the product yield was 2.0 g of abscisic acid/liter of fermentation broth. After ion exchange and recrystallization recovery, the product recovery rate is 82%.
  • Comparative Example 5 A continuous flow fed feed discharge process was employed. 30 bottles of 1000 ml flasks were used, and 300 ml of medium A per bottle was sterilized at 12 CTC.
  • the activated abscisic acid high-yield strain TB-3-H8 was sprayed, and the spore suspension was placed at a temperature of about 26 ° C.
  • the flask was incubated for 40-56 hours.
  • the cultured first-class seed solution is inoculated into a 100-liter fermenter (second-stage seed tank) containing 50 liters of medium B at a 15%-20% inoculum, and aeration is stirred at a temperature of 26 ° C - 27 ° C. Cultivate for 24-26 hours.
  • Three-stage tank fermentation was carried out with a 1 ton fermenter.
  • the tank was filled with medium C of about 500 L and microporous ceramic beads (particle size of 0.9-160 mm3, 1.
  • the seed tank of the second tank is inoculated with 10%-15% inoculation amount, and the fermentation is stirred for 20-48 hours, then the chlorine is introduced to control the pH to 3-8, and the fermentation is lowered.
  • the temperature is 10 ° C - 25 ° C, and the medium C is continuously fed at a rate of 0.01 to 5 L / h, continuously at a rate of 0.5 - 1. 0 yg / L * h (uniform or non-uniform) Add inositol. Discharge once every 10 hours.
  • the fermentation temperature was about 27 ° C and the fermentation pH was 4-7.
  • the medium combination used in the course of the implementation was the same as in Comparative Example 4.
  • the fermentation time is up to 25 days when the cans are placed.
  • the next tank fermentation broth recovers the product by ion exchange resin method, elutes with ethanol, and recrystallizes to obtain white Crystalline abscisic acid product.
  • the contamination rate of the bacteria is 30.0%
  • the product yield is 1.6 g of abscisic acid/liter of fermentation broth.
  • the product recovery rate reached 82%.
  • Example 6 50 pieces of 1000 ml flasks were used, and 300 ml of medium A per bottle was sterilized at 12 CTC.
  • the activated abscisic acid high-yield strain Botrytis cinerea TBC-10 CGMCC No. 1889 spore suspension was inoculated. Incubate at 25 ° (temperature, shake flask for 24-33 hours. Inoculate the cultured first-class seed solution at a 15% '-18% inoculum in a 200 liter fermentor containing 100 liters of medium B (secondary Seed tank), agitated and cultured for 24 to 30 hours at a temperature of about 26 ° C.
  • Three-stage tank fermentation with 1 ton fermenter, medium C of about 500 L in tank, sterilized by conventional high-pressure steam sterilization Inoculate the secondary tank seed solution according to the inoculation amount of 20% - 25%, and agitate and ferment the fermentation. After 15-24 hours, the feed medium C is added intermittently, and the intermittent time is fed every 20-28 hours. 3 times, each feeding amount is 0. 05% - 0. 3 ° /.. until about 10 hours before stopping the fermentation (under can).
  • the medium used in the implementation is as follows: Glucose 3. 0% ⁇ 0. 0% ⁇ 0. 0% Ammonium nitrate 1. 5% Magnesium sulphate 0. 05% Maltose 0.
  • the fermentation temperature was about 27 ° C and the fermentation pH was 4-7.
  • the can is fermented for 9 days.
  • the can fermentation broth was recovered by ion exchange resin method, eluted with ethanol, and recrystallized to obtain a white crystalline abscisic acid product.
  • the fermentation system After testing: After 9 days of stable fermentation, the fermentation system has a contamination rate of 5. 0% and a product yield of 2. 0 g of abscisic acid per liter of fermentation broth. After ion exchange and recrystallization recovery, the product recovery rate is 85%.

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Abstract

L'invention porte sur un procédé de préparation d'acide abscissique naturel par introduction d'inositol pour déréprimer la répression du glucose pendant la fermentation, et sur la nouvelle source bactérienne pour le procédé. Le procédé comprend les étapes suivantes consistant à : cultiver des champignons produisant de l'acide abscissique naturel dans le milieu de culture liquide primaire, inoculer le liquide d'ensemencement primaire cultivé dans le milieu de culture liquide secondaire, et cultiver, introduire le milieu de culture pendant la fermentation tertiaire et introduire de l'inositol simultanément, et récupérer l'acide abscissique à partir du bouillon fermenté.
PCT/CN2007/000268 2007-01-24 2007-01-24 Nouveau procédé de préparation d'acide abscissique naturel WO2008092297A1 (fr)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102329739A (zh) * 2011-08-31 2012-01-25 云南大学 一株用于发酵小桐子饼粕制作生物菌肥的杂色曲霉菌
CN102424799A (zh) * 2011-12-27 2012-04-25 云南大学 一株用于发酵小桐子饼粕制作脱毒饲料的杂色曲霉菌
US8389024B2 (en) 2005-06-22 2013-03-05 Gonzalo Romero M. Abscisic acid against cancer
CN113981015A (zh) * 2021-12-17 2022-01-28 四川龙蟒福生科技有限责任公司 一种降低发酵生产s-aba杂质的工艺方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1182798A (zh) * 1996-11-18 1998-05-27 中国科学院成都生物研究所 真菌发酵生产天然脱落酸的新方法
CN1355318A (zh) * 2000-11-27 2002-06-26 中国科学院成都生物研究所 制备天然活性脱落酸的方法

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1182798A (zh) * 1996-11-18 1998-05-27 中国科学院成都生物研究所 真菌发酵生产天然脱落酸的新方法
CN1355318A (zh) * 2000-11-27 2002-06-26 中国科学院成都生物研究所 制备天然活性脱落酸的方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
ZHANG H.C. ET AL.: "Effects of INO1 Gene Expression in Schizosaccharomyces pombe on Invertase Secretion and Phosphoinositol (PI) Synthesis", JOURNAL OF SHANDONG UNIVERSITY (NATURAL SCIENCE), vol. 37, no. 6, December 2002 (2002-12-01), pages 544 - 547, 551 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8389024B2 (en) 2005-06-22 2013-03-05 Gonzalo Romero M. Abscisic acid against cancer
CN102329739A (zh) * 2011-08-31 2012-01-25 云南大学 一株用于发酵小桐子饼粕制作生物菌肥的杂色曲霉菌
CN102424799A (zh) * 2011-12-27 2012-04-25 云南大学 一株用于发酵小桐子饼粕制作脱毒饲料的杂色曲霉菌
CN113981015A (zh) * 2021-12-17 2022-01-28 四川龙蟒福生科技有限责任公司 一种降低发酵生产s-aba杂质的工艺方法

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