WO2007116474A1 - 醤油香の少ない醸造醤油及びその製造法 - Google Patents
醤油香の少ない醸造醤油及びその製造法 Download PDFInfo
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- WO2007116474A1 WO2007116474A1 PCT/JP2006/306866 JP2006306866W WO2007116474A1 WO 2007116474 A1 WO2007116474 A1 WO 2007116474A1 JP 2006306866 W JP2006306866 W JP 2006306866W WO 2007116474 A1 WO2007116474 A1 WO 2007116474A1
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- soy sauce
- moromi
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- fermentation
- yeast
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
- A23L27/50—Soya sauce
Definitions
- the present invention relates to a novel soy sauce that has a rich fragrance with little soy sauce flavor despite being brewed soy sauce.
- Soy sauce is one of the traditional seasonings in Japan, and soy or soybean and wheat grains are heat-denatured, adjusted to moisture, inoculated with koji molds and cultured as soy sauce koji. This is prepared by adding (mixing) saline to the soy sauce moromi, fermenting and ripening, squeezing and filtering to form a clear liquid, and firing (heat sterilization).
- soy sauce thus obtained is referred to as “brewed soy sauce”.
- Brewing soy sauce requires a process of long-term fermentation and ripening for 3 to 10 months, and during this time it has a unique color, taste and aroma due to the action of various enzymes and microorganisms in soy sauce cake, soy sauce moromi become a thing.
- its aroma is known to have more than 300 kinds of ingredients, and these are involved in complex ways to create a unique flavor of soy sauce.
- moromi prepared while suppressing yeast fermentation, and soy sauce non-fermented moromi obtained by high-temperature digestion for a short time are inferior in masking power with strong raw material odor and warm odor, Almost no production and accumulation occurs, so there is a shortage of alcohol, there is a problem with antifungal properties, and the use value as a seasoning becomes low.
- soy sauce moromi soup with or without yeast fermentation has a monotonous flavor with a lack of aging that makes it difficult to expect a complex taste derived from yeast.
- soy sauce with excellent versatility can be obtained by reducing only the soy sauce flavor.
- brewed soy sauce various aroma components that form the rich scent of soy sauce are mainly produced and accumulated by soy sauce yeast and are also referred to as "brewed scent".
- alcohol-based fragrance components represented by methionol have the effect of reducing raw material odors such as steamed soybeans and fried rice crackers, which have a high correlation with yeast fermentation, and soy sauce odor. It is known that it has an effect of eliminating (unmasking) the unpleasant scent of various ingredients and seasoning materials when used as (Non-patent Document 2).
- the soy sauce moromi that has undergone proper yeast fermentation contains about 20-150ppm of HEMF per 1.0% of total nitrogen (WZV), and at the same time methionol is 1.0-3. Contains about Oppm (Non-patent Document 3).
- soy sauce does not want a soy sauce-like or soy-derived aroma that has a strong scent of soy sauce! /, Suitable for use in processed foods! /.
- amino acid soy sauce obtained by hydrolyzing defatted soybeans with hydrochloric acid, neutralizing with alkali and then pressing and filtering is known as soy sauce not containing HEMF.
- the amino acid soy sauce has the same object as the present invention in that it does not contain HEMF, but is different from the soy sauce of the present invention in that it does not contain metionol.
- Patent Document 1 Japanese Patent Laid-Open No. 3-183490
- Patent Document 2 Japanese Patent Laid-Open No. 5-176781
- Patent Document 3 Japanese Patent Laid-Open No. 6-277083
- Patent Document 4 JP-A-5-115261
- Patent Document 5 JP-A-9-271351
- Patent Document 6 Japanese Unexamined Patent Application Publication No. 2004-141014
- Non-Patent Document 1 Soy Sauce Series Vol. 1 by Ichiro Nagase, 1967, 1967, P2 9
- Non-patent document 2 Research report No. 8NOVEMVER. 2000, Numazu Industrial Technology Center, Shizuoka Prefecture, Development of new seasoning materials using microorganisms
- Non-Patent Document 3 Augmented Soy Sauce Science and Technology, edited by Shinro Itakura, 1994 (May 31, 1994), P285
- the problem of the present invention is that it is a brewed soy sauce, but has a little scent of soy sauce and a rich aroma. It is to obtain new soy sauce.
- the present inventors have heat-denatured soybean seeds or plant seeds such as soybeans and wheat, adjusted water, and inoculated and cultured aspergillus.
- Soy sauce koji mixed with salt water and mixed with soy sauce moromi, fermented and matured, squeezed and filtered to form a clear liquid, and baked (heat sterilized) to produce brewed soy sauce
- the salt water draw ratio is set to a significantly higher value (170 to 450% (VZV)) than that of the conventional method (about 120% (VZV)), and the soy sauce during the fermentation ripening It was found that when soy sauce cake or soy sauce cake and saline solution were added again to moromi and fermented and matured, soy sauce with little soy sauce flavor and rich flavor was obtained.
- brewed soy sauce produced by such a characteristic method has 4-hydroxy-2 (or 5) ethyl-5 (or 2) methyl-3 (2H) per 1.0% (WZV) of total nitrogen. It was found that it contains furanone at 15. Oppm or less and methionol at 2. Oppm or more. And based on these findings! / The present invention was completed.
- the first aspect of the present invention is:
- the present invention relates to a method for producing brewed soy sauce with less soy flavor, characterized by adding soy sauce cake or soy sauce cake and saline to the soy sauce moromi in the middle of fermentation and aging, and further fermenting and aging the soy sauce moromi after the addition.
- the second aspect of the present invention is:
- the volume of plant seeds used for the preparation of soy sauce cake (replaced as raw seeds)
- the volume ratio of the saline solution to the calculated volume) is referred to as the “pumped water ratio” of the saline solution.
- the pumping rate is (saline volume Z raw seed volume)
- X 100 (10L / 8L )
- X 100 125% (VZV).
- the volume of raw seed means a “bulk volume” that includes a void portion and is measured using a graduated cylinder or the like.
- a novel soy sauce having a rich fragrance with little soy sauce aroma can be obtained even though it is a brewed soy sauce.
- soy sauce having a masking effect to eliminate unpleasant scents of various ingredients and seasoning materials can be obtained.
- soy sauce with excellent aftertaste can be obtained.
- a very light soy sauce can be obtained even though the pumping rate is the same.
- the process from the start of fermentation ripening of soy sauce moromi obtained by adding a predetermined amount of saline to soy sauce cake until the addition of soy sauce cake or soy sauce cake and saline solution Is sometimes referred to as the “first stage fermentation ripening step”.
- the soy sauce moromi in the first stage fermentation ripening process is sometimes referred to as “first moromi”.
- soy sauce cake or soy sauce cake and saline are added to the first stage moromi in the middle of the first stage fermentation ripening step, that is, before the fermentation ripening is completed.
- soy sauce koji is added to the first stage moromi
- the soy sauce koji added to the first stage moromi may be particularly referred to as “additional koji”.
- soy sauce cake and saline are added to the first stage moromi
- the soy sauce cake and saline are mixed in advance to make soy sauce moromi, and added to the first stage moromi in the form of soy sauce moromi.
- the soy sauce moromi a mixture of soy sauce cake and saline
- added to the first moromi may be referred to as “additional moromi”.
- the step of further fermenting soy sauce moromi after adding additional koji or additional moromi to the first stage moromi may be referred to as “second stage fermentation aging process”.
- the soy sauce moromi in the “second stage fermentation ripening process” may be referred to as “second moromi”.
- cooked protein ingredients such as soybeans and defatted soybeans, and starch ingredients such as wheat (wheat, barley, barley, and wheat) and rice are heated.
- starch ingredients such as wheat (wheat, barley, barley, and wheat) and rice are heated.
- seed gonococcus such as Aspergillus 'soya, Aspergillus' oryzae.
- the heat denaturation of wheat is performed by fried rice crackers.
- Heat denaturation of rice is preferably performed by steaming or cooking.
- plant seeds used for the preparation of soy sauce lees include protein raw materials such as soybeans, defatted soybeans, and wheat (wheat, barley, bare wheat, hato barley), rice It is a general term for starchy raw materials.
- raw seed refers to the plant seed before being subjected to treatments such as drying, heating, pulverization, and steaming.
- raw wheat raw material weight (kg) raw wheat raw material volume (L) X O. 75.
- the weight per unit volume may be calculated as a coefficient.
- the concentration of the salt solution in the second-stage moromi after completion of the second-stage fermentation ripening step is preferably 13.0 to 20.0% (WZV). 0% (WZV) is more preferred, 15. 0-17. 0 0 / o (W / V) power most preferred! / ⁇ .
- the salt concentration in the first-stage moromi is the same as that in the second-stage moromi.
- the salt solution used for the preparation is a salt solution with an amount of 170 to 450% (V / V). That is, the sum of the volume (i) when the protein material, which is the raw material of soy sauce cake, is converted into raw seeds, and the volume (mouth), when the starchy material, which is the raw material of the soy sauce cake, is converted into raw seeds.
- V / V the volume of 170-450%
- the first stage moromi prepared under the above conditions is subjected to lactic acid fermentation at a temperature of 10 to 20 ° C. with or without the addition of lactic acid bacteria. At this time, it is desirable to avoid aeration as much as possible and manage at a low temperature of 20 ° C or less so that soy sauce yeast fermentation does not precede.
- the lactic acid fermentation is preferably performed until 0.5% (WZV) or more of lactic acid is produced in the soy sauce moromi.
- WZV 0.5%
- the soy sauce in which lactic acid is produced and accumulated is preferable because it has a good flavor as soy sauce.
- Soy sauce moromi (pH 5.8 to 6.2) obtained by adding soy sauce cake to saline gradually dissolves (hydrolyzes), and elution of raw materials and lactic acid fermentation are started. Decrease to ⁇ 4.5.
- the soy sauce moromi is suitable for yeast growth.
- Soy sauce yeast is preferably added after the end of lactic acid fermentation, that is, when the pH of the moromi falls below 5.3, in order to prevent premature springing.
- the soy sauce yeast used for yeast fermentation in the present invention has a salt concentration of 10.0 to 20.
- soy sauce yeast that can vigorously grow and breed in 0% (W / V) soy sauce moromi.
- soy sauce yeast examples include Zygosaccharomyces rouxii, Candida (Torulopsis) genus yeast, C. etchellsii, C. verstilis and the like.
- the yeast In order to promote rapid fermentation, the yeast should be cultured in advance so that the initial bacterial count is 1 x 10 5 to 1 x 10 7 per lg of moromi. Add the yeast to soy sauce moromi. I prefer to do that.
- yeast fermentation keeps moromi aerobic.
- the present inventors aerobically maintain the soy sauce moromi which has a significantly higher value (170 to 450% (VZV)) of the pumping rate at the time of charging the first stage moromi.
- VZV very low value
- the soy sauce moromi produced in this way has a considerably high pumping rate compared to ordinary soy sauce moromi. Therefore, compared with the conventional method, the soy sauce moromi mashing ratio (about 120% (VZV)), the soy sauce moromi has a lower solid content and the moromi viscosity is lower. Since the amount is small, it has the advantage that coloring of moromi and the scattering of alcohol can be minimized.
- the amount of HEMF produced can be suppressed to a prescribed amount or less despite the vigorous growth of yeast. preferable.
- soy sauce By completing the first stage fermentation and ripening process and directly obtaining the first stage moromi, you can obtain soy sauce with less soy sauce flavor. However, there is no thickness for the taste with low total nitrogen concentration. In addition, it is difficult to manage moromi because there is a risk of contamination by harmful microorganisms with low alcohol concentration. Furthermore, since the moromi is too soft, it is difficult to squeeze it with a normal squeezing device, and a special squeezing device is required. Moreover, since it ferments under aerobic conditions, it lacks a rich scent and has a problem of yeast odor when ripened.
- the second stage moromi is prepared, that is, the first stage moromi is in the middle of the first stage fermentation ripening process.
- soy sauce cake additional rice cake
- soy sauce cake and salt water additional moromi
- the purpose of the additional mash or the additional moromi soup for the first moromi is to compensate for the lack of total nitrogen and alcohol in the first moromi. There is no need to use any special soup sauce or soy sauce cake for the preparation of additional moromi.
- the amount of additional koji or additional moromi to be used can be appropriately determined according to the pumping rate at the time of charging the first stage moromi.
- the amount of additional koji or additional moromi used is preferably such that the total nitrogen concentration in the finally obtained soy sauce product is equivalent to that of ordinary brewed soy sauce. Therefore, it is preferable to adjust the final pumping rate (total pumping rate) within the range of 80 to 160% (VZV). In particular, the range of 120 to 140% (VZV) is most preferable because it is excellent in both yield and quality.
- “Final pumping ratio” or “total pumping ratio” is the ratio of the total volume of saline to the total volume of plant seed material used throughout the manufacturing process of the final product, brewed soy sauce. .
- the “final scoop ratio” or “total scoop ratio” is the raw material of soy sauce koji for the first stage moromi preparation.
- the volume of saline solution for the preparation of the first stage moromi relative to the total volume of the plant seed material volume (raw seed equivalent) and the volume of plant seed material (raw seed equivalent) Means the ratio of Further, in the embodiment in which additional moromi is added to the first stage moromi, the “final scoop ratio” or “total scoop ratio” is the plant seed that is the raw material of the soy sauce cake for the preparation of the first stage moromi.
- Salt for the preparation of the first stage moromi relative to the total volume of the material volume (raw seed conversion) and the plant seed material volume (raw seed conversion) that is the raw material of the soy sauce cake for the preparation of additional moromi It means the ratio of the total volume of the volume of water and the volume of saline for the preparation of additional moromi.
- the ratio of the additional koji or the soy sauce koji for the preparation of the additional moromi to the soy sauce koji for the preparation of the first stage moromi may be 50 to 300% (WZW). If this ratio is 50% (WZW) or less, even if additional koji is added instead of additional moromi, the total nitrogen concentration in the finally obtained brewed soy sauce will be low, such being undesirable. On the other hand, if this ratio exceeds 300% (WZW), the elution of the raw material of the second stage moromi is not good, and the raw material utilization rate decreases, which is not preferable.
- this percentage is in the range of 50 to 300% (WZW) Has an appropriate moromi viscosity, and can obtain a good quality moromi without pressing problems. In particular, in the range of 100 to 200% (WZW), it is preferable that the balance of quality is good.
- the concentration of the saline used for the preparation of the additional moromi depends on the salt concentration of the first moromi and the mixing ratio of the soy sauce cake and the saline used for the preparation of the additional moromi.
- the concentration of the soy sauce moromi (second stage moromi) after addition may be such that the salt concentration is 13.0 to 20.0% (WZV).
- the timing of the addition of additional koji or additional moromi is not strictly stipulated, but in the first moromi, the number of viable yeast is 3 x 10 7 or more per lg of the first moromi, especially 5 X 10 It is desirable to add during the period when 7 or more are reached. 1st stage moromi No more than 3 x 10 7 per lg, or enter dead state, and the number of viable yeast is less than S3 x 10 7 Addition of moromi is not preferable because the rate of sugar source accumulation by elution of raw materials exceeds the rate of sugar source utilization by yeast and HEMF is produced.
- the number of viable cells of soy sauce yeast in moromi is measured using a salt-resistant yeast count medium (soy sauce test method, edited by Japan Soy Sauce Research Institute, March 1, 1985, see P248) Add the specified amount of moromi dilution diluted to the specified concentration to the following modified medium supplemented with chloramfecoal and measure the number of soy yeast colonies after culturing at 30 ° C for 24 hours. Obtain the number of viable yeast (individual Zg moromi).
- Powdered yeast 2g KH PO: lg
- the moromi after addition of additional koji or additional moromi is further fermented and ripened.
- this step it is preferable to complete fermentation and ripening by the action of the yeast and lactic acid bacteria that have transferred to the first stage of moromi, without adding new lactic acid bacteria and yeast.
- the elution of the sugar source and the utilization of the sugar source by the yeast that has transferred to the first stage of moromi power are performed almost simultaneously. In other words, alcohol is produced and accumulated without significantly increasing the sugar concentration in the moromi. As a result, the amount of HEMF produced can be minimized.
- This fermentation also improves the flavor balance of moromi and improves the microbial stability of moromi. About aeration after addition of additional moromi, it is desirable to carry it out moderately because alcohol will scatter when carried out vigorously.
- the second-stage moromi obtained by adding additional calorie koji or additional moromi in the middle of the first-stage fermentation ripening process cannot be expected for lactic acid fermentation after the addition. It is preferable to allow fermentation to produce 0.5% (W / V) or more of lactic acid.
- the management of the second stage moromi in the second stage fermentation ripening process may be in accordance with a general moromi management method. However, when HEMF is prepared at a low temperature, it is preferable to perform low temperature control at 25 ° C or lower. .
- management at 20 ° C or lower is more preferable because the amount of HEMF produced can be suppressed to almost zero.
- soy sauce lactic acid bacteria so that the initial concentration is IX 10 5 Zg moromi, and control the moromi while keeping the moromi product temperature at 15 ° C, and elution of raw materials and lactic acid fermentation 1 month of gourmet! 1st stage moromi (yeast added moromi) having the component analysis values shown in Table 1 was obtained.
- salt-resistant soy sauce yeast (Tigo Saccharomyces 'Luxi') was added so that the initial bacterial count was 5 x 10 5 Zg moromi, and the aroma was stirred at 20 ° C (air flow: 1. Ovvm ) For 10 days and had the number of yeasts listed in Table 1, and HEMF and methionol each obtained the first moromi listed in Table 1.
- the second stage moromi was fermented and matured for 2 months at a moromi product temperature of 20-25 ° C.
- the second stage moromi after fermentation and ripening was squeezed and filtered by a conventional method to obtain a final product (soy sauce) having the component analysis values shown in Table 2.
- Comparative Examples of Category 12 and 13 shown in Table 3 are examples in which the soy sauce was manufactured by completing the fermentation ripening of the first stage moromi without adding additional moromi.
- the brewed soy sauce of the present invention (category 5-9), which has a salt water draw rate of 170-450% (V ZV) when preparing the first stage moromi, Compared to the control brewed soy sauce (Category 3), which is used in the manufacturing method of brewed soy sauce of 120% (V / V), the HEMF concentration is significantly reduced. The concentration should increase by a factor of 2-3.
- Ramen soup was prepared by the following recipe using each soy sauce product obtained above.
- Pork bone hot water extract concentrate (S & B company solid soup “Shirayu soup”) 25g
- the sensory test was performed by 20 trained panels with discriminating ability, and the number of panels (results) that felt soy soy sauce was stronger than the control (Category 3). This shows the number of panel members who felt that (a) was strong (results) and the number of panel members (results) who felt that the soup's aftertaste grew strongly.
- Table 3 shows the results of the sensory test.
- soy sauce obtained from the amino acid soy sauce of the comparative example (category 11) and the soy sauce obtained from the first stage moromi of the comparative example (category 12 and 13) were compared with the control example (category 3), soy sauce stock solution, ramen soup Although the soy sauce feeling was weak, the ramen soup, which had a bad flavor in the soy sauce stock solution, was noticeable. Also, the aftertaste growth in ramen soup is equal to or less than that of the comparative example (Category 3), and it can be seen that the aftertaste growth of the soy sauce of the present invention is not far away.
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Priority Applications (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN2006800303332A CN101242747B (zh) | 2006-03-31 | 2006-03-31 | 酱油香少的酿造酱油及其制造方法 |
JP2008509625A JP4781428B2 (ja) | 2006-03-31 | 2006-03-31 | 醤油香の少ない醸造醤油及びその製造法 |
PCT/JP2006/306866 WO2007116474A1 (ja) | 2006-03-31 | 2006-03-31 | 醤油香の少ない醸造醤油及びその製造法 |
EP06730814A EP2002735B1 (en) | 2006-03-31 | 2006-03-31 | Fermented soy sauce with less soy sauce flavor and process for producing the same |
US12/064,279 US8173185B2 (en) | 2006-03-31 | 2006-03-31 | Fermented soy sauce with less soy sauce flavor and process for producing the same |
TW095135304A TWI364259B (en) | 2006-03-31 | 2006-09-25 | Brewing soy sauce with few smell of soy sauce and the preparation method thereof |
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PCT/JP2006/306866 WO2007116474A1 (ja) | 2006-03-31 | 2006-03-31 | 醤油香の少ない醸造醤油及びその製造法 |
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WO2007116474A1 true WO2007116474A1 (ja) | 2007-10-18 |
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US (1) | US8173185B2 (ja) |
EP (1) | EP2002735B1 (ja) |
JP (1) | JP4781428B2 (ja) |
CN (1) | CN101242747B (ja) |
TW (1) | TWI364259B (ja) |
WO (1) | WO2007116474A1 (ja) |
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JP2015019598A (ja) * | 2013-07-16 | 2015-02-02 | サッポロビール株式会社 | 非アルコール飲料及びその製造方法、並びに非アルコール飲料へのアルコール感付与方法 |
JP2015023848A (ja) * | 2013-07-29 | 2015-02-05 | キッコーマン株式会社 | トマト含有調味料 |
JP2015100351A (ja) * | 2013-11-28 | 2015-06-04 | キッコーマン株式会社 | 卵及び乳を含有する調味料 |
JP2016214145A (ja) * | 2015-05-20 | 2016-12-22 | キッコーマン株式会社 | 煮付け用調味液 |
JP2017165879A (ja) * | 2016-03-16 | 2017-09-21 | キッコーマン株式会社 | 不快臭軽減剤 |
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EP2805623B1 (en) * | 2009-09-18 | 2016-03-09 | Kikkoman Corporation | Low common salt soy sauce and process for producing same |
US20140004225A1 (en) * | 2011-03-23 | 2014-01-02 | Kikkoman Corporation | Very low common salt soy sauce and method for producing same |
JP6433670B2 (ja) | 2013-05-31 | 2018-12-05 | ヤマサ醤油株式会社 | 醸造醤油および醸造醤油の製造方法 |
RU2596028C1 (ru) * | 2015-04-14 | 2016-08-27 | Федеральное Государственное Автономное Образовательное Учреждение Высшего Профессионального Образования "Дальневосточный Федеральный Университет" (Двфу) | Соевый соус |
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CN110558537A (zh) * | 2019-10-15 | 2019-12-13 | 韦立 | 一种茉莉花香酱油的制作方法 |
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- 2006-03-31 JP JP2008509625A patent/JP4781428B2/ja active Active
- 2006-03-31 EP EP06730814A patent/EP2002735B1/en active Active
- 2006-03-31 WO PCT/JP2006/306866 patent/WO2007116474A1/ja active Application Filing
- 2006-03-31 US US12/064,279 patent/US8173185B2/en active Active
- 2006-09-25 TW TW095135304A patent/TWI364259B/zh active
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JP2015012811A (ja) * | 2013-07-03 | 2015-01-22 | キッコーマン株式会社 | チキンエキス含有スープ |
JP2015019598A (ja) * | 2013-07-16 | 2015-02-02 | サッポロビール株式会社 | 非アルコール飲料及びその製造方法、並びに非アルコール飲料へのアルコール感付与方法 |
JP2015023848A (ja) * | 2013-07-29 | 2015-02-05 | キッコーマン株式会社 | トマト含有調味料 |
JP2015100351A (ja) * | 2013-11-28 | 2015-06-04 | キッコーマン株式会社 | 卵及び乳を含有する調味料 |
JP2016214145A (ja) * | 2015-05-20 | 2016-12-22 | キッコーマン株式会社 | 煮付け用調味液 |
JP2017165879A (ja) * | 2016-03-16 | 2017-09-21 | キッコーマン株式会社 | 不快臭軽減剤 |
JP2020534851A (ja) * | 2017-09-29 | 2020-12-03 | サムヤン コーポレイション | 醤油組成物およびその製造方法 |
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JPWO2007116474A1 (ja) | 2009-08-20 |
EP2002735B1 (en) | 2011-09-14 |
TW200744477A (en) | 2007-12-16 |
EP2002735A2 (en) | 2008-12-17 |
TWI364259B (en) | 2012-05-21 |
US20090098246A1 (en) | 2009-04-16 |
JP4781428B2 (ja) | 2011-09-28 |
EP2002735A9 (en) | 2009-04-08 |
CN101242747A (zh) | 2008-08-13 |
CN101242747B (zh) | 2012-08-22 |
EP2002735A4 (en) | 2010-07-14 |
US8173185B2 (en) | 2012-05-08 |
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