WO2007111283A1 - グリコヘモグロビン濃度測定方法および濃度測定装置 - Google Patents
グリコヘモグロビン濃度測定方法および濃度測定装置 Download PDFInfo
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- WO2007111283A1 WO2007111283A1 PCT/JP2007/056111 JP2007056111W WO2007111283A1 WO 2007111283 A1 WO2007111283 A1 WO 2007111283A1 JP 2007056111 W JP2007056111 W JP 2007056111W WO 2007111283 A1 WO2007111283 A1 WO 2007111283A1
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- glycated hemoglobin
- light
- concentration
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- measuring
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/62—Detectors specially adapted therefor
- G01N30/74—Optical detectors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/314—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry with comparison of measurements at specific and non-specific wavelengths
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
- G01N33/721—Haemoglobin
- G01N33/723—Glycosylated haemoglobin
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N2021/3129—Determining multicomponents by multiwavelength light
- G01N2021/3137—Determining multicomponents by multiwavelength light with selection of wavelengths after the sample
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/314—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry with comparison of measurements at specific and non-specific wavelengths
- G01N2021/3144—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry with comparison of measurements at specific and non-specific wavelengths for oxymetry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
- G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
- G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
- G01N2030/8822—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving blood
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
- G01N21/05—Flow-through cuvettes
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
Definitions
- the present invention relates to a method for measuring the concentration of glycohemoglobin contained in a sample such as blood.
- HPLC device high performance liquid chromatography device
- HPLC high performance liquid chromatography
- the general HPLC apparatus 9 prepares a sample containing a biological component in the sample preparation unit 90 and then introduces the sample into the analytical column 91.
- the biological component is analyzed by the analytical column 91. It is configured to be adsorbed on the filler.
- a biological sample in which hemolyzed blood is diluted is introduced into the analytical column 91 after hemolyzing red blood cells collected from the whole blood.
- the biological component adsorbed on the filler is eluted by supplying the eluent to the eluent bottle 93 force analysis column 91 by the liquid feed pump 92.
- the eluent from the analysis column 91 is introduced into the photometric mechanism 94, and the absorbance of the eluent is continuously measured by the photometric mechanism 94 to analyze the biological components.
- the photometric mechanism 94 irradiates light from the light source 97 while the eluent flows through the flow path 96 of the photometric cell 95, and the transmitted light at that time is received by the light receiving unit 98. It receives light. While the wavelength of the light received by the light receiving unit 98 is selected by the Chiken filter 99, the light receiving unit 98 outputs an output level signal corresponding to the amount of received light.
- the HPLC apparatus 9 is further based on a chromatogram, which is a change in absorbance over time! / The total amount of hemoglobin is calculated, and the concentration of glycohemoglobin is calculated as the proportion of glycohemoglobin in the total amount of hemoglobin.
- the photometric mechanism 94 has a maximum absorption of oxyhemoglobin.
- the wavelength of 415 nm is used as the measurement wavelength. For this reason, the ratio of oxyhemoglobin and deoxyhemoglobin is different in environments where the environmental temperature varies greatly, making accurate measurement difficult when measuring them at the same wavelength. It becomes.
- Patent Document 1 Japanese Patent Application Laid-Open No. 7-120447
- the present invention has an object to make it possible to appropriately measure the concentration of projectohemoglobin even when the ratio between oxyhemoglobin and deoxyhemoglobin is different. Means for Solving the Problems
- a method for measuring glycohemoglobin concentration by an optical method wherein the molecular absorption coefficients of oxyhemoglobin and deoxyhemoglobin match or substantially match.
- a method for measuring the concentration of glycated hemoglobin at the wavelength is provided.
- the measurement wavelength is set to 417 to 427 nm. More preferably, the measurement wavelength is set to 419 to 425 nm.
- the measurement wavelength is not limited to the previous range, and other wavelength ranges known as wavelengths where the molecular extinction coefficients of oxyhemoglobin and deoxyhemoglobin match or approximately match, such as 520-526 nm or 583 It may be set to ⁇ 589nm.
- the present invention can be applied to the measurement of glycohemoglobin concentration using a column chromatography and using a sample in which the erythrocyte power in blood is also prepared.
- glycated hemoglobin concentration measurement is provided with a photometric mechanism that irradiates a sample with light from a light source and receives light traveling from the sample at that time in a light receiving unit.
- the photometric mechanism is configured so that light having a wavelength at which the molecular extinction coefficients of oxyhemoglobin and deoxyhemoglobin match or substantially match can be received by the light receiving unit.
- a glycated hemoglobin concentration measuring device is provided.
- the photometric mechanism is configured such that, for example, light of 417 to 427 nm can be received by the light receiving unit.
- the photometry mechanism is configured so that light of 419 to 425 nm can be received by the light receiving unit.
- the photometry mechanism may also be configured so that light of 520 to 526 nm or 583 to 589 nm can be received by the light receiving unit.
- the glycated hemoglobin concentration measuring apparatus of the present invention further includes a separation unit for separating glycated hemoglobin using, for example, column chromatography.
- the glycated hemoglobin concentration measuring device of the present invention further includes a sample preparation mechanism for preparing a sample from red blood cells in blood!
- FIG. 1 is a schematic configuration diagram showing an HPLC apparatus which is an example of a glycohemoglobin measuring apparatus according to the present invention.
- FIG. 2 is a cross-sectional view for explaining a photometric mechanism in the HPLC apparatus shown in FIG.
- FIG. 3 is a graph showing the wavelength dependence of molecular extinction coefficients of oxyhemoglobin and deoxyhemoglobin.
- FIG. 4 is a block diagram showing a main part of the HPLC apparatus shown in FIG. 1.
- FIG. 5 is a flowchart for explaining the operation of the HPLC apparatus shown in FIG. 1.
- FIG. 6 is a flowchart for explaining concentration calculation processing in a calculation circuit in the HPLC apparatus shown in FIG. 1.
- FIG. 7 is an example of a chromatogram obtained with the HPLC apparatus shown in FIG. 1.
- FIG. 8 is a graph showing the relationship between environmental temperature and glycated hemoglobin concentration in Example 1
- FIG. 9 is a graph showing the relationship between environmental temperature and glycated hemoglobin concentration in Example 2
- FIG. 10 is a schematic configuration diagram showing an HPLC apparatus as an example of a conventional glycated hemoglobin measuring apparatus.
- FIG. 11 is a cross-sectional view for explaining a photometric mechanism in the HPLC apparatus shown in FIG. Explanation of symbols
- the HPLC apparatus X shown in FIG. 1 corresponds to an example of the glycated hemoglobin concentration measuring apparatus of the present invention, and is configured to measure the glycated hemoglobin concentration using whole blood.
- the HPLC apparatus X includes a plurality of eluent bottles 10, 11, 12 (three in the drawing), a degassing device 2, a sample preparation unit 3, an analysis unit 4, a photometric mechanism 5, and an arithmetic circuit 6.
- Each eluent bottle 10, 11, 12 holds an eluent to be supplied to an analysis column 40 described later.
- eluents for example, kaffers with different pH and salt concentrations are used.
- the degassing device 2 is for removing dissolved gas from the eluent before supplying the eluent to the analysis unit 4 (analytical column 40), and is connected via pipes 70A, 70B, and 70C.
- the eluent bottles 10, 11, 12 are connected to the Mayunoredo 41 of the analysis unit 4 via self-tubes 71A, 71B, 71C.
- the sample preparation unit 3 is for preparing a sample to be introduced into the analysis column 40 from the blood cell component of the blood sample 14 collected from the blood collection tube 13.
- This sample preparation unit 3 has a sampling nozzle 30, a preparation liquid tank 31, and a dilution tank 32. ing.
- the sampling nozzle 30 is for collecting various liquids including the blood sample 14 in the blood collection tube 13.
- the sampling nozzle 30 is capable of sucking and discharging the liquid and moving in the vertical and horizontal directions. It is possible.
- the operation of the sampling nozzle 30 is controlled by a control means (not shown).
- the preparation liquid tank 31 holds a preparation liquid for preparing a sample for introduction to be introduced into the analytical column 40 based on the blood sample 14.
- This preparation liquid tank 31 holds a hemolysis agent for hemolyzing red blood cells, a dilution liquid for diluting the hemolysis, and the like as preparation liquids.
- the dilution tank 32 is used to provide a place for lysing red blood cells in the blood sample 14 and preparing a sample for introduction by diluting the hemolyzed blood.
- This dilution tank 32 is connected to an injection valve 43 in an analysis unit 4 to be described later via a pipe 72.
- the sample force for introduction prepared in the dilution tank 32 is connected to the analysis column 40 via the injection valve 43. It is configured to be introduced.
- the analysis unit 4 controls the adsorption and elution of biological components with respect to the packing material of the analytical column 40, and supplies various biological components to the photometric mechanism 5.
- the temperature is controlled by.
- the set temperature in the analysis unit 4 is about 40 ° C, for example.
- the analytical column 40 holds a filler for selectively adsorbing hemoglobin in the sample.
- the filler for example, a methacrylic acid ester copolymer is used.
- the analysis unit 4 includes a hold 41, a liquid feed pump 42, and an injection valve 43.
- the merge 41 is used for selectively supplying an eluent from a specific eluent bottle 10 0, 11, 12 of the plurality of eluent bottles 10, 11, 12 to the injection valve 43. It is.
- the hold 41 is connected to the deaeration device 2 via pipes 71A, 71B, 71C, and connected to the injection valve 43 via a pipe 73 !.
- the liquid feed pump 42 is for applying power for moving the eluent to the analysis column 40 via the injection valve 43, and is provided in the middle of the pipe 73. Sending The liquid pump 42 is operated, for example, so that the flow rate of the eluent becomes 1.0 to 2. OmlZmin.
- the injection valve 43 collects a fixed amount of the sample for introduction and allows the sample for introduction to be introduced into the analysis column 40.
- the injection valve 43 includes a plurality of introduction ports and discharge ports (not shown). Yes.
- An injection loop 44 is connected to the injection valve 43.
- the injection loop 44 is capable of holding a fixed amount (for example, several / z L) of liquid, and the injection loop 44 communicates with the dilution tank 32 from the dilution tank 32 by appropriately switching the injection valve 43.
- an injection valve 43 for example, a hexagonal noble can be used.
- the photometric mechanism 5 is for optically detecting hemoglobin contained in the eluent from the analytical column 40, and includes a photometric cell 50, a light source 51, and a beam splitter. 5 2. It has a light receiving system 53 for measurement and a light receiving system 54 for reference.
- the photometric cell 50 is for defining a photometric area.
- This photometric cell 50 has an introduction channel 50A, a photometry channel 50B, and a discharge channel 50C, and these channels 50A, 50B, and 50C communicate with each other.
- the introduction channel 50A is used to introduce the eluent from the analysis column 40 (see FIG. 1) into the photometric channel 50B, and is connected to the analysis column 40 via a pipe 75.
- the photometric flow path 50B circulates the eluent to be measured and provides a field for photometric measurement of the eluent, and is formed in a straight line.
- the photometric flow path 50B is open at both ends, and both ends are closed by the transparent cover 55.
- the discharge channel 50C is for discharging the eluent from the photometric channel 50B, and is connected to the waste liquid tank 15 via a pipe 76 (see FIG. 1).
- the light source 51 is for irradiating light to the eluent flowing through the photometric flow path 50B.
- the light source 51 is arranged in a state of facing the end face 50Ba (transparent cover 55) of the photometric flow path 50B so that the optical axis L passes through the center of the photometric flow path 50B.
- a light having a measurement wavelength that matches or substantially matches the molecular extinction coefficient of hemoglobin and deoxyhemoglobin, and one that can emit light having a reference wavelength are used.
- measurement wavelength A light having a measurement wavelength that matches or substantially matches the molecular extinction coefficient of hemoglobin and deoxyhemoglobin, and one that can emit light having a reference wavelength are used.
- the force measurement wavelength which can mention the wavelength range of 417-426 nm may be 520-526 nm or 583-589 nm.
- 500 nm can be adopted as the reference wavelength.
- a halogen lamp can be used as the light source 51 capable of emitting light of the measurement wavelength and the reference wavelength in the previous wavelength range.
- the light source 51 may be other than a halogen lamp, for example, one having one or a plurality of LED elements.
- the beam splitter 52 divides the light emitted from the light source 51 and transmitted through the photometric flow path 50B so as to be incident on the measurement light receiving system 53 and the reference light receiving system 54. On the optical axis L, it is arranged in a state inclined by 45 degrees.
- the beam splitter 52 various known ones such as a half mirror can be used.
- the light receiving system 53 for measurement selectively receives light having a target wavelength out of the light transmitted through the beam splitter 52 and is disposed on the optical axis L.
- the measurement light receiving system 53 includes an interference filter 53A and a light receiving element 53B for receiving light transmitted through the interference filter 53A.
- a photodiode can be used as the light receiving element 53B.
- the interference filter 53A selectively transmits light having a target measurement wavelength.
- the measurement wavelength force is set to 17 to 426 nm, preferably 419 to 423 nm. This is due to the following reason.
- the molecular extinction coefficient of oxyhemoglobin and the molecular extinction coefficient of deoxyhemoglobin match when the wavelength is approximately 421 nm, so the measurement wavelength is 421 nm or a wavelength close to it.
- the hemoglobin concentration in the hemoglobin contained in the eluent from the analytical column 40 can be measured without being affected by the ratio of oxyhemoglobin to deoxyhemoglobin or its fluctuation. It is possible.
- the interference filter 53A is one that selectively transmits light in the wavelength range of 520 to 526 nm or 583 to 589 nm.
- the reference light receiving system 54 shown in FIG. 2 is for acquiring data for suppressing the turbidity and scattering effect of the eluent from the analysis column 40.
- the reference light receiving system 54 shown in FIG. Of the light that has been reflected and whose optical path has been changed, it selectively receives light with a reference wavelength of 500 nm.
- the measurement light receiving system 74 includes an interference filter 54A that selectively transmits 500 nm light, and a light receiving element 54B that receives the light transmitted through the interference filter 54A.
- a photodiode can be used as the light receiving element 54B.
- the arithmetic circuit 6 includes a control unit 60 and a calculation unit 61.
- the control unit 60 is for controlling the operation of each unit. More specifically, the control unit 60 controls turning on and off of the light source 51, controls the interference filter 53A, selects the light receiving element 53B, and selects the wavelength of light to be received. Controls density calculation processing in unit 61.
- the calculation unit 61 is for calculating the glycohemoglobin concentration in whole blood based on the light reception results of the light receiving elements 53B and 54B.
- the calculation unit 61 stores a program necessary for calculation, and its operation is controlled by the control unit 60.
- the eluent is supplied to the analytical column 40 (S2).
- the eluent is supplied from the eluent bottles 10, 11 and 12 to the injection valve 43 through the degassing device 2 and the hold 41 by the power of the liquid feed pump 42, and is also supplied to a plurality of eluent bottles 10, 11 and 11. Which of the 12 eluent bottles 10, 11, 12 is supplied is selected by controlling the manifold 41.
- the lysate supplied to the injection valve 43 is supplied to the analysis column 40 via the pipe 74.
- a sample for introduction to be introduced into the analytical column 40 is further prepared (S3).
- a blood sample 14 is first collected from the blood collection tube 13. Collection of the blood sample 14 from the blood collection tube 13 is performed by operating the sampling nozzle 30. The blood sample 14 collected by the sampling nozzle 30 is supplied to the dilution tank 32 by operating the sampling nozzle 30. Further, a hemolyzing agent and a diluent are sequentially supplied from the preparation tank 31 to the dilution tank 32, and a sample for introduction is prepared by mixing the liquid in the dilution tank 32 by pipetting operation using the sampling nozzle 30. .
- the sample for introduction is introduced into the analytical column 40 (S4).
- the introduction of the sample for introduction into the analytical column 40 is performed by causing the eluent to flow through the injection loop 44 by switching the injection valve 43. That is, the sample for introduction of the injection valve 44 is introduced into the analysis column 40 together with the eluent.
- glycohemoglobin is adsorbed on the filler by introducing the sample for introduction. After glycated hemoglobin is adsorbed on the packing material, the type of eluent supplied to the analytical column 40 is switched appropriately by the hold 41 to elute the glycated hemoglobin adsorbed on the packing material.
- the eluent containing glycohemoglobin discharged from the analytical column 40 is supplied to the photometric cell 50 of the photometric mechanism 5 through the pipe 76 and photometrically measured (S6).
- S6 photometrically measured
- eluent from analytical column 40 via pipe 75 and inlet channel 50A
- the eluent is guided to the waste liquid tank 15 via the pipe 76 so that the force shown in FIG.
- the photometric mechanism 5 when the eluent from the analysis column 40 passes through the photometric channel 50B, the light source 51 continuously irradiates the eluent with light.
- the light transmitted through the photometric channel 50B is split by the beam splitter 52 and then received by the measurement light receiving system 53 and the reference light receiving system 54.
- the measurement light receiving system 53 the light transmitted through the interference filter 53A is selectively received by the light receiving element 53B.
- the reference light receiving system 54 light having a reference wavelength of 500 ⁇ m that has passed through the interference filter 54A is selectively received by the light receiving element 54 ⁇ .
- the light reception results of the light receiving elements 53B and 54B are output to the arithmetic circuit 6, and the concentration of glycohemoglobin is calculated in the arithmetic circuit 6 (S7).
- the concentration calculation processing in the arithmetic circuit 6 is performed according to the procedure of the flowchart shown in FIG.
- the absorbance corresponding to hemoglobin is accumulated (S 10), and the absorbance corresponding to glycohemoglobin (the portion indicated by cross-hatching in Fig. 7) is accumulated (S 1 Do
- the concentration of glycated hemoglobin is calculated using a wavelength at which the molecular extinction coefficient of oxyhemoglobin and the molecular extinction coefficient of deoxyhemoglobin match or substantially coincide with each other as a measurement wavelength.
- the wavelength at which the molecular extinction coefficients of oxyhemoglobin and deoxyhemoglobin match is constant without being affected by the ratio of oxyhemoglobin to deoxyhemoglobin, and glycohemoglobin at such wavelengths.
- the present invention is not limited to the embodiment described above, and various modifications can be made.
- the force that the amount of hemoglobin was acquired as the absorbance The amount of hemoglobin that is not necessarily acquired as the absorbance, or simply as the amount of received light. Good.
- the configuration for causing the light receiving element 53B to receive light having a target wavelength is limited to the configuration using the interference filter 53A. Alternatively, other known methods can be employed.
- the present invention is not limited to an HPLC apparatus for measuring the concentration of glycated hemoglobin in blood, but is also applicable to a liquid chromatographic apparatus other than the HPLC apparatus or other glycated hemoglobin concentration measuring apparatus when a sample other than blood is used. It can also be applied to.
- Example 1
- the concentration of glycated hemoglobin was measured using the glycated hemoglobin measuring device ("ADAMS Ale HA-8160” manufactured by ARKRAY, Inc.) when the environmental temperature was set to 10 ° C, 20 ° C and 30 ° C.
- a photo diode array (“UV-visible multi-wavelength detector MD-910J; manufactured by JASCO Corporation)" was used as the light receiving element.
- Glycohemoglobin concentration was lnm in the wavelength range of 415 to 430nm. Each time, it was calculated as the ratio of the amount of glycohemoglobin in the total amount of hemoglobin.
- the measurement wavelength was 423 nm
- the measured values were almost the same regardless of the environmental temperature.
- the measurement wavelength is in the range of 416 to 427 nm
- the measurement wavelength is the maximum absorption wavelength of oxyhemoglobin.
- the measurement value was stable without being affected by the environmental temperature. In particular, when the measurement wavelength was 419 to 426 nm, it was even more unaffected by the environmental temperature.
- the measured values almost coincided regardless of the environmental temperature when the measurement wavelength was 423 nm.
- the measurement wavelength was in the range of 417 to 427 nm, the measurement wavelength was the maximum absorption wavelength of oxyhemoglobin, which was stronger than the influence of the ambient temperature than when it was 415 nm.
- the measurement wavelength was 419 to 425 nm, it was even more unaffected by the environmental temperature.
- the measurement wavelength is set to 415 nm, which is the maximum absorption wavelength of oxyhemoglobin, and the measurement wavelength is the same as that in Example 1! Best ever!
- the result was set to 423 ⁇ m, the influence of the environmental temperature on the measured value was examined.
- the measured value of glycated hemoglobin was measured in the same manner as in Example 1 for each of the healthy subject specimen and the diabetic patient specimen.
- the measurement results of glycohemoglobin when the measurement wavelength is set to 415 nm are shown in Table 3 and FIG. 9A below, and the measurement results when the measurement wavelength is set to 423 nm are shown in Table 4 and FIG. 9B below.
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Abstract
Description
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Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2008507483A JP4989628B2 (ja) | 2006-03-24 | 2007-03-23 | グリコヘモグロビン濃度測定方法および濃度測定装置 |
CN2007800187891A CN101484793B (zh) | 2006-03-24 | 2007-03-23 | 糖基血红蛋白浓度测定方法和浓度测定装置 |
US12/225,526 US8021887B2 (en) | 2006-03-24 | 2007-03-23 | Method of measuring glycated hemoglobin concentration |
EP07739552.3A EP2015053B1 (en) | 2006-03-24 | 2007-03-23 | Method for determination of glycosylated hemoglobin level and apparatus for determination of the level |
Applications Claiming Priority (2)
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JP2006082101 | 2006-03-24 | ||
JP2006-082101 | 2006-03-24 |
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WO2007111283A1 true WO2007111283A1 (ja) | 2007-10-04 |
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US (1) | US8021887B2 (ja) |
EP (1) | EP2015053B1 (ja) |
JP (1) | JP4989628B2 (ja) |
CN (1) | CN101484793B (ja) |
WO (1) | WO2007111283A1 (ja) |
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JP2020101426A (ja) * | 2018-12-21 | 2020-07-02 | 東ソー株式会社 | 吸光光度検出器 |
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US9041933B2 (en) * | 2012-03-01 | 2015-05-26 | Schlumberger Technology Corporation | System and method for characterizing crude oil fractions |
CN106290168A (zh) * | 2015-05-18 | 2017-01-04 | 深圳迈瑞生物医疗电子股份有限公司 | 光检测单元、使用该光检测单元的液相色谱分析装置及液相色谱分析方法 |
US10527594B2 (en) * | 2016-04-20 | 2020-01-07 | Arkray, Inc. | Liquid chromatography measurement method, liquid chromatography measurement instrument, and liquid chromatography measurement program storage medium |
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JPWO2007111283A1 (ja) | 2009-08-13 |
CN101484793A (zh) | 2009-07-15 |
EP2015053A4 (en) | 2014-03-05 |
EP2015053A1 (en) | 2009-01-14 |
EP2015053B1 (en) | 2016-06-15 |
US8021887B2 (en) | 2011-09-20 |
JP4989628B2 (ja) | 2012-08-01 |
CN101484793B (zh) | 2012-07-04 |
US20090317912A1 (en) | 2009-12-24 |
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