WO2004044574A8 - Trennvorrichtung und trennverfahren für biomolekulares probenmaterial - Google Patents

Trennvorrichtung und trennverfahren für biomolekulares probenmaterial

Info

Publication number
WO2004044574A8
WO2004044574A8 PCT/EP2003/012320 EP0312320W WO2004044574A8 WO 2004044574 A8 WO2004044574 A8 WO 2004044574A8 EP 0312320 W EP0312320 W EP 0312320W WO 2004044574 A8 WO2004044574 A8 WO 2004044574A8
Authority
WO
WIPO (PCT)
Prior art keywords
separating
substance sample
sample
constituents
biomolecular substance
Prior art date
Application number
PCT/EP2003/012320
Other languages
English (en)
French (fr)
Other versions
WO2004044574A1 (de
Inventor
Albert Roeder
Alois Rainer
Hans-Joachim Hoeltke
Carlo Effenhauser
Peter Berndt
Hanno Langen
Remo Hochstrasser
Original Assignee
Hoffmann La Roche
Roche Diagnostics Gmbh
Albert Roeder
Alois Rainer
Hans-Joachim Hoeltke
Carlo Effenhauser
Peter Berndt
Hanno Langen
Remo Hochstrasser
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche, Roche Diagnostics Gmbh, Albert Roeder, Alois Rainer, Hans-Joachim Hoeltke, Carlo Effenhauser, Peter Berndt, Hanno Langen, Remo Hochstrasser filed Critical Hoffmann La Roche
Priority to JP2004550931A priority Critical patent/JP4541894B2/ja
Priority to AU2003301992A priority patent/AU2003301992A1/en
Priority to EP03810966A priority patent/EP1558925A1/de
Priority to CA2505255A priority patent/CA2505255C/en
Publication of WO2004044574A1 publication Critical patent/WO2004044574A1/de
Priority to US11/120,098 priority patent/US7645369B2/en
Publication of WO2004044574A8 publication Critical patent/WO2004044574A8/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44773—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)

Abstract

Die Erfindung betrifft ein Trennvorrichtung und ein Trennverfahren für biomolekulares Probenmaterial, insbesondere Proteingemische. Hierfür ist ein Trennelement (10) zur zweidimensionalen vorzugsweise elektrophoretischen Separation von Komponenten des Probenmaterials im Bereich (30) einer Trennebene vorgesehen. Erfindungsgemäß wird vorgeschlagen, dass das Trennelement (10) eine Kanal- oder Transferstruktur (14) zum ortsaufgelösten Ausschleusen von separierten Probenkomponenten in einer quer zu der Trennebene verlaufenden Transportrichtung auf eine für massenspektroskopische Untersuchungen geeignete Trägerfläche (16) aufweist.
PCT/EP2003/012320 2002-11-09 2003-11-05 Trennvorrichtung und trennverfahren für biomolekulares probenmaterial WO2004044574A1 (de)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP2004550931A JP4541894B2 (ja) 2002-11-09 2003-11-05 生体分子サンプル物質の分離デバイスおよび分離方法
AU2003301992A AU2003301992A1 (en) 2002-11-09 2003-11-05 Device for separating and method for separating a biomolecular substance sample
EP03810966A EP1558925A1 (de) 2002-11-09 2003-11-05 Trennvorrichtung und trennverfahren für biomolekulares probenmaterial
CA2505255A CA2505255C (en) 2002-11-09 2003-11-05 Separation device and separation method for biomolecular sample material
US11/120,098 US7645369B2 (en) 2002-11-09 2005-05-02 Separation method and device for protein mixtures

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10252177A DE10252177A1 (de) 2002-11-09 2002-11-09 Trennvorrichtung und Trennverfahren für biomolekulares Probenmaterial
DE10252177.8 2002-11-09

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US11/120,098 Continuation US7645369B2 (en) 2002-11-09 2005-05-02 Separation method and device for protein mixtures

Publications (2)

Publication Number Publication Date
WO2004044574A1 WO2004044574A1 (de) 2004-05-27
WO2004044574A8 true WO2004044574A8 (de) 2005-12-15

Family

ID=32308504

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2003/012320 WO2004044574A1 (de) 2002-11-09 2003-11-05 Trennvorrichtung und trennverfahren für biomolekulares probenmaterial

Country Status (7)

Country Link
US (1) US7645369B2 (de)
EP (1) EP1558925A1 (de)
JP (1) JP4541894B2 (de)
AU (1) AU2003301992A1 (de)
CA (1) CA2505255C (de)
DE (1) DE10252177A1 (de)
WO (1) WO2004044574A1 (de)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7105810B2 (en) 2001-12-21 2006-09-12 Cornell Research Foundation, Inc. Electrospray emitter for microfluidic channel
US7537807B2 (en) 2003-09-26 2009-05-26 Cornell University Scanned source oriented nanofiber formation
CA2612052A1 (en) * 2005-06-18 2006-12-28 Ge Healthcare Bio-Sciences Ab Method and devices for forming a plurality of wells on a gel
US7989215B2 (en) 2005-06-18 2011-08-02 Ge Healthcare Bio-Sciences Ab Methods and systems for adding a reagent to an analyte in a gel
KR100792683B1 (ko) * 2006-05-09 2008-01-09 연세대학교 산학협력단 모세관등전집중-중공사흐름장흐름분획법을 이용한 단백질분리장치 및 분리방법
JPWO2008078403A1 (ja) * 2006-12-26 2010-04-15 日本電気株式会社 電気泳動チップ及びその使用方法
DE102008020428B3 (de) * 2008-04-24 2009-07-16 Johannes-Gutenberg-Universität Mainz Vorrichtung und Verfahren und Gelsystem zur analytischen und präparativen Elektrophorese
JP6803918B2 (ja) 2015-11-10 2020-12-23 ウッドハム バイオテクノロジー ホールディングス エルエルシー 導電性高分子を用いたゲル電気泳動法及び転写法の組合せ並びにその使用方法
US9702851B1 (en) 2016-06-17 2017-07-11 Woodham Biotechnology Holdings, LLC Gel electrophoresis and transfer combination using conductive polymers and method of use
WO2023248280A1 (ja) * 2022-06-20 2023-12-28 株式会社東陽テクニカ マイクロチップ、２次元試料分離システム及びマイクロチップの製造方法

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3704217A (en) * 1969-09-08 1972-11-28 Samuel T Nerenberg Segmental macromolecular separation method and apparatus
US4181594A (en) * 1979-03-27 1980-01-01 University Of Pittsburgh Matrix recovery electrophoresis apparatus
US4693804A (en) * 1984-12-19 1987-09-15 Board Of Regents, The University Of Texas System Apparatus for bidimensional electrophoretic separations
GB8523801D0 (en) * 1985-09-26 1985-10-30 Jones K W Vacuum molecular transfer(blotting)apparatus
CA1330966C (en) * 1987-07-24 1994-07-26 Leo G. Woerner Process and apparatus for conducting electrophoresis and transfer
US5039493A (en) * 1990-05-04 1991-08-13 The United States Of America As Represented By The Secretary Of The Navy Positive pressure blotting apparatus with hydropholic filter means
DE4114611A1 (de) * 1990-05-11 1991-11-14 Sartorius Gmbh Flaches mikroporoeses membranelement, insbesondere zur durchfuehrung von slot- oder dot-blottinganalysen
US5279721A (en) * 1993-04-22 1994-01-18 Peter Schmid Apparatus and method for an automated electrophoresis system
DE4408034C1 (de) * 1994-03-10 1995-07-13 Bruker Franzen Analytik Gmbh Verfahren zur massenspektrometrischen Analyse von Proben aus 2D-Gel-Elektrophoreseplatten mit matrixunterstützter ionisierender Laser-Desorption
JPH08233799A (ja) * 1995-02-24 1996-09-13 Tefuko Kk 化学分析用膜およびその製造方法
US5580434A (en) * 1996-02-29 1996-12-03 Hewlett-Packard Company Interface apparatus for capillary electrophoresis to a matrix-assisted-laser-desorption-ionization mass spectrometer
US6013165A (en) * 1998-05-22 2000-01-11 Lynx Therapeutics, Inc. Electrophoresis apparatus and method

Also Published As

Publication number Publication date
CA2505255C (en) 2010-10-26
US20060000712A1 (en) 2006-01-05
WO2004044574A1 (de) 2004-05-27
JP2006505786A (ja) 2006-02-16
CA2505255A1 (en) 2004-05-27
JP4541894B2 (ja) 2010-09-08
US7645369B2 (en) 2010-01-12
EP1558925A1 (de) 2005-08-03
AU2003301992A1 (en) 2004-06-03
DE10252177A1 (de) 2004-06-09

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