ES2121054T3 - Analisis de muestras por inmunosustraccion electroforetica capilar. - Google Patents

Analisis de muestras por inmunosustraccion electroforetica capilar.

Info

Publication number
ES2121054T3
ES2121054T3 ES93303805T ES93303805T ES2121054T3 ES 2121054 T3 ES2121054 T3 ES 2121054T3 ES 93303805 T ES93303805 T ES 93303805T ES 93303805 T ES93303805 T ES 93303805T ES 2121054 T3 ES2121054 T3 ES 2121054T3
Authority
ES
Spain
Prior art keywords
capillary electrophoretic
samples
aliquot
sample
parts
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES93303805T
Other languages
English (en)
Inventor
Ching-Ming Liu
Hann-Ping Wang
Fu-Tai Albert Chen
James C Sternberg
Gerald L Klein
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Beckman Coulter Inc
Original Assignee
Beckman Coulter Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Beckman Coulter Inc filed Critical Beckman Coulter Inc
Application granted granted Critical
Publication of ES2121054T3 publication Critical patent/ES2121054T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G01N27/44726—Arrangements for investigating the separated zones, e.g. localising zones by optical means using specific dyes, markers or binding molecules

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Electrochemistry (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Electrochromic Elements, Electrophoresis, Or Variable Reflection Or Absorption Elements (AREA)
  • Cephalosporin Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)

Abstract

SE DESCRIBEN METODOLOGIAS PARA INMUNOSUBSTRACCION ELECTROFORETICA CAPILAR ("CEI") DE MUESTRAS. EN UNA VERSION PREFERENTE, CEI DE UNA MUESTRA COMPRENDIENDO PARTES CONSTITUYENTES A SEPARAR, COMPRENDE LAS FASES DE: (1) SEPARAR UNA PRIMERA PARTE ALICUOTA DE LA MUESTRA DENTRO DE LAS PARTES CONSTITUYENTES MEDIANTE TECNICAS ELECTROFORETICAS CAPILARES, Y DETECTANDO LAS CITADAS PARTES; (2) MEZCLANDO UNA SEGUNDA PARTE ALICUOTA DE LA CITADA MUESTRA CON, AL MENOS, UNA COMPAÑERA AGLUTINANTE ESPECIFICA A UN CONSTITUYENTE DE LA CITADA MUESTRA, SIENDO LA CITADA COMPAÑERA AGLUTINANTE ESPECIFICA CAPAZ DE SER EXTRAIDA SUSTANCIALMENTE DE LA CITADA PARTE ALICUOTA; (3) SEPARANDO LA CITADA SEGUNDA PARTE ALICUOTA EN PARTES CONSTITUYENTES MEDIANTE TECNICAS ELECTROFORETICAS CAPILARES Y DETECTANDO LAS CITADAS PARTES; Y (4) COMPARANDO LAS PARTES CONSTITUYENTES SEPARADAS DE LA FASE (3) CON LAS PARTES CONSTITUYENTES SEPARADAS DE LA FASE (1).
ES93303805T 1992-07-17 1993-05-17 Analisis de muestras por inmunosustraccion electroforetica capilar. Expired - Lifetime ES2121054T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/916,313 US5228960A (en) 1992-07-17 1992-07-17 Analysis of samples by capillary electrophoretic immunosubtraction

Publications (1)

Publication Number Publication Date
ES2121054T3 true ES2121054T3 (es) 1998-11-16

Family

ID=25437045

Family Applications (1)

Application Number Title Priority Date Filing Date
ES93303805T Expired - Lifetime ES2121054T3 (es) 1992-07-17 1993-05-17 Analisis de muestras por inmunosustraccion electroforetica capilar.

Country Status (7)

Country Link
US (1) US5228960A (es)
EP (1) EP0579361B1 (es)
JP (1) JP2589265B2 (es)
AT (1) ATE169737T1 (es)
CA (1) CA2096389C (es)
DE (1) DE69320273T2 (es)
ES (1) ES2121054T3 (es)

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GB9301122D0 (en) * 1993-01-21 1993-03-10 Scient Generics Ltd Method of analysis/separation
GB9306729D0 (en) * 1993-03-31 1993-05-26 British Tech Group Improvements in separators
US5622675A (en) * 1993-04-16 1997-04-22 Beckman Instruments, Inc. Sample segment
NL9300965A (nl) * 1993-06-04 1995-01-02 Proteios B V Werkwijze voor het bij een proefobject detecteren van de aanwezigheid van ten minste een hapteen, een macromolecuul, een pathogeen of immuunreactie opwekkend deel daarvan, alsmede een dergelijke werkwijze voor het detecteren van een eventuele immuunreactie.
US5567282A (en) * 1994-01-25 1996-10-22 Wang; Hann-Ping On-capillary electrophoretic immunosubtraction for classification and typing of M-proteins
JPH08511874A (ja) * 1994-04-13 1996-12-10 ベックマン インスツルメンツ インコーポレーテッド 生物学的流体中の検体の分析
US5536382A (en) * 1994-05-23 1996-07-16 Advanced Molecular Systems, Inc. Capillary electrophoresis assay method useful for the determination of constituents of a clinical sample
US5431793A (en) * 1994-07-29 1995-07-11 Beckman Instruments, Inc. Quantitative analysis of glycosylated hemoglobin by immunocappillary electrophoresis
DE69633962T2 (de) * 1995-01-27 2005-12-01 Northeastern University, Boston Verfahren zum Bilden einer kovalent gebundenen hydrophilen Schicht auf Basis von Polyvinyl-Alkohol für die Kapillarelektrophorese
GB9507125D0 (en) * 1995-04-06 1995-05-31 Scient Generics Ltd Travelling wave electrodes
US5800692A (en) * 1995-04-17 1998-09-01 Mayo Foundation For Medical Education And Research Preseparation processor for use in capillary electrophoresis
US5753094A (en) * 1995-09-20 1998-05-19 Beckman Instruments, Inc. Borate storage buffer and sample diluent
US6299747B1 (en) 1997-12-17 2001-10-09 Cetek Corporation Capillary electrophoretic methods to detect new biologically active compounds in complex biological material
EP1042668A1 (en) * 1997-12-24 2000-10-11 Cetek Corporation Capillary electrophoretic method to detect target-binding ligands and to determine their relative affinities
US6156179A (en) * 1998-07-09 2000-12-05 Bio-Rad Laboratories Computer directed identification of paraproteins
US6432651B1 (en) 1998-07-10 2002-08-13 Cetek Corporation Method to detect and analyze tight-binding ligands in complex biological samples using capillary electrophoresis and mass spectrometry
US6680208B1 (en) 1999-11-19 2004-01-20 Becton, Dickinson And Company Rapid protein identification using antibody mixtures
US6974527B2 (en) * 2000-06-06 2005-12-13 Spectrumedix Llc Multidimensional separations employing an array of electrophoresis channels
US7691645B2 (en) * 2001-01-09 2010-04-06 Agilent Technologies, Inc. Immunosubtraction method
FR2862134B1 (fr) * 2003-11-12 2007-07-27 Sebia Sa Analyse et typage de proteines monoclonales par electrophorese capillaire et immunodeplacement
US7935479B2 (en) 2004-07-19 2011-05-03 Cell Biosciences, Inc. Methods and devices for analyte detection
US7846676B2 (en) 2004-07-19 2010-12-07 Cell Biosciences, Inc. Methods and devices for analyte detection
GB0501741D0 (en) * 2005-01-27 2005-03-02 Binding Site The Ltd Antibody
JP4742712B2 (ja) * 2005-07-14 2011-08-10 株式会社島津製作所 キャピラリー電気泳動方法
US8945361B2 (en) * 2005-09-20 2015-02-03 ProteinSimple Electrophoresis standards, methods and kits
EP2049233A4 (en) 2006-07-10 2011-08-31 Convergent Bioscience Ltd METHOD AND DEVICE FOR PRECISELY SELECTION AND EXTRACTION OF A FOCUSED COMPONENT IN ISOLELECTRIC FOCUSING PERFORMED IN MICRO CHANNELS
CA2646944A1 (en) * 2007-11-30 2009-05-30 Her Majesty The Queen In Right Of Canada, As Represented By The Minister Of National Defence Serum components that bind to threat agents
US10107782B2 (en) * 2008-01-25 2018-10-23 ProteinSimple Method to perform limited two dimensional separation of proteins and other biologicals
GB201010405D0 (en) 2010-06-21 2010-08-04 Binding Site Group The Ltd Assay
US9804079B2 (en) 2012-04-19 2017-10-31 ProteinSimple Dual wavelength isoelectric focusing for determining drug load in antibody drug conjugates
US9766206B2 (en) 2013-09-27 2017-09-19 ProteinSimple Apparatus, systems, and methods for capillary electrophoresis
CN109696505A (zh) * 2017-10-23 2019-04-30 康普药业股份有限公司 一种精确称取微量易挥发溶剂的方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4246085A (en) * 1979-02-16 1981-01-20 United Kingdom Atomic Energy Authority Fractionation of proteins
JPS56138249A (en) * 1980-03-31 1981-10-28 Shimadzu Corp Cataphoresis measuring method of cell
US4668363A (en) * 1984-03-16 1987-05-26 Beckman Instruments, Inc. Immunofixation electrophoresis process
JPS60205263A (ja) * 1984-03-30 1985-10-16 Kureha Chem Ind Co Ltd 電気泳動法による細胞の検査方法
JPS61280570A (ja) * 1985-06-06 1986-12-11 Ken Hirohashi 電気泳動による新規な分析方法
JPH03173510A (ja) * 1989-12-04 1991-07-26 Matsushita Electric Ind Co Ltd 調理器の外かく
EP0533909B1 (en) * 1991-03-28 1997-01-08 Perseptive Biosystems, Inc. On-line product identification in a chromatography effluent by subtraction
JPH0587813A (ja) * 1991-04-15 1993-04-06 Fuji Yakuhin Kogyo Kk 電気泳動によるｍタンパクのクラス同定方法
US5120413A (en) * 1991-05-31 1992-06-09 Beckman Instruments, Inc. Analysis of samples utilzing capillary electrophoresis

Also Published As

Publication number Publication date
JPH0735753A (ja) 1995-02-07
CA2096389C (en) 2002-07-09
CA2096389A1 (en) 1994-01-18
DE69320273T2 (de) 1998-12-17
EP0579361B1 (en) 1998-08-12
EP0579361A3 (en) 1994-12-07
JP2589265B2 (ja) 1997-03-12
EP0579361A2 (en) 1994-01-19
ATE169737T1 (de) 1998-08-15
US5228960A (en) 1993-07-20
DE69320273D1 (de) 1998-09-17

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