WO2001040497A3 - Procede d'obtention d'acides nucleiques a partir d'un echantillon de l'environnement - Google Patents

Procede d'obtention d'acides nucleiques a partir d'un echantillon de l'environnement Download PDF

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Publication number
WO2001040497A3
WO2001040497A3 PCT/FR2000/003311 FR0003311W WO0140497A3 WO 2001040497 A3 WO2001040497 A3 WO 2001040497A3 FR 0003311 W FR0003311 W FR 0003311W WO 0140497 A3 WO0140497 A3 WO 0140497A3
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WIPO (PCT)
Prior art keywords
nucleic acids
novel
nucleic acid
obtaining
concerns
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PCT/FR2000/003311
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English (en)
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WO2001040497A2 (fr
Inventor
Pascale Jeannin
Jean-Luc Pernodet
Michel Guerineau
Pascal Simonet
Sophie Courtois
Carmela Cappellano
Francois Francou
Alain Raynal
Maria Ball
Guennadi Sezonov
Karine Tuphile
Asa Frostegard
Original Assignee
Aventis Pharma Sa
Pascale Jeannin
Jean-Luc Pernodet
Michel Guerineau
Pascal Simonet
Sophie Courtois
Carmela Cappellano
Francois Francou
Alain Raynal
Maria Ball
Guennadi Sezonov
Karine Tuphile
Asa Frostegard
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from FR9915032A external-priority patent/FR2801609B1/fr
Priority to NZ519883A priority Critical patent/NZ519883A/en
Priority to AU21791/01A priority patent/AU781961B2/en
Priority to CA002393041A priority patent/CA2393041A1/fr
Priority to MXPA02003819A priority patent/MXPA02003819A/es
Priority to KR1020027006787A priority patent/KR20020060242A/ko
Priority to EP00985340A priority patent/EP1268764A2/fr
Priority to JP2001542561A priority patent/JP2003520578A/ja
Application filed by Aventis Pharma Sa, Pascale Jeannin, Jean-Luc Pernodet, Michel Guerineau, Pascal Simonet, Sophie Courtois, Carmela Cappellano, Francois Francou, Alain Raynal, Maria Ball, Guennadi Sezonov, Karine Tuphile, Asa Frostegard filed Critical Aventis Pharma Sa
Priority to BR0015993-0A priority patent/BR0015993A/pt
Priority to IL14984600A priority patent/IL149846A0/xx
Priority to US10/148,328 priority patent/US7255989B1/en
Publication of WO2001040497A2 publication Critical patent/WO2001040497A2/fr
Priority to NO20022532A priority patent/NO20022532L/no
Publication of WO2001040497A3 publication Critical patent/WO2001040497A3/fr
Priority to AU2005211587A priority patent/AU2005211587A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P33/00—Antiparasitic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P33/00—Antiparasitic agents
    • A61P33/02—Antiprotozoals, e.g. for leishmaniasis, trichomoniasis, toxoplasmosis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/06—Immunosuppressants, e.g. drugs for graft rejection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Veterinary Medicine (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Genetics & Genomics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biophysics (AREA)
  • Transplantation (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

La présente invention concerne un procédé de préparation d'acides nucléiques à partir d'un échantillon de l'environnement, plus particulièrement un procédé d'obtention d'une collection d'acides nucléiques à partir d'un échantillon. L'invention est également relative aux acides nucléiques ou aux collections d'acides nucléiques obtenus selon le procédé et leur application à la synthèse de nouveaux composés, notamment de nouveaux composés d'intérêt thérapeutique. L'invention a également pour objet les moyens nouveaux mis en oeuvre dans le procédé d'obtention d'acides nucléiques ci-dessus, tels que de nouveaux vecteurs et des nouveaux procédés de préparation de tels vecteurs ou encore des cellules hôtes recombinantes comprenant un acide nucléique de l'invention. L'invention concerne encore des procédés pour détecter un acide nucléique d'intérêt au sein d'une collection d'acides nucléiques obtenus selon le procédé ci-dessus, ainsi que les acides nucléiques détectés par un tel procédé et les polypeptides codés par de tels acides nucléiques.
PCT/FR2000/003311 1999-11-29 2000-11-27 Procede d'obtention d'acides nucleiques a partir d'un echantillon de l'environnement WO2001040497A2 (fr)

Priority Applications (12)

Application Number Priority Date Filing Date Title
US10/148,328 US7255989B1 (en) 1999-11-29 2000-11-27 Method for obtaining nucleic acids from an environment sample, resulting nucleic acids and use in synthesis of novel compounds
JP2001542561A JP2003520578A (ja) 1999-11-29 2000-11-27 環境サンプルからの核酸の入手方法、得られた核酸、および新規化合物の合成における用途
CA002393041A CA2393041A1 (fr) 1999-11-29 2000-11-27 Procede d'obtention d'acides nucleiques a partir d'un echantillon de l'environnement, acides nucleiques ainsi obtenus et leur application a la synthese de nouveaux composes
MXPA02003819A MXPA02003819A (es) 1999-11-29 2000-11-27 Procedimiento de obtencion de acidos nucleicos a partir de una muestra del medio ambiente, acidos nucleicos asi obtenidos y su aplicacion en la sintesis de compuestos.
KR1020027006787A KR20020060242A (ko) 1999-11-29 2000-11-27 환경 샘플로부터 핵산의 수득방법, 수득된 핵산 및 신규화합물 합성에서의 이의 용도
EP00985340A EP1268764A2 (fr) 1999-11-29 2000-11-27 Procede d'obtention d'acides nucleiques a partir d'un echantillon de l'environnement
BR0015993-0A BR0015993A (pt) 1999-11-29 2000-11-27 Processos de preparação de uma coleção de ácidos nucléicos, de uma coleção de vetores recombinantes e de um vetor recombinante, de detecção de um ácido nucléico e de uma policetìdeo sintase, de identificação da produção de um composto de interesse, de seleção de uma célula hospedeira recombinante, de produção de um composto de interesse e de uma policetìdeo sintase e de determinação da diversidade de ácidos nucléicos contidos em uma coleção, coleção de ácidos nucléicos, ácido nucléico, vetor, coleção de vetores, célula hospedeira recombinante, coleção de células hospedeiras recombinantes, composto, policetìdeo, composição, policetìdeo sintase, anticorpo e kit de detecção de uma policetìdeo sintase
NZ519883A NZ519883A (en) 1999-11-29 2000-11-27 Extraction of DNA from microorganisms in dry soil samples thereby identifying the diversity of the organisms present
AU21791/01A AU781961B2 (en) 1999-11-29 2000-11-27 Method for obtaining nucleic acids from an environment sample, resulting nucleic acids and use in synthesis of novel compounds
IL14984600A IL149846A0 (en) 1999-11-29 2000-11-27 Method for obtaining nucleic acids from an evironment sample, resulting nucleic acids and use in synthesis of novel compounds
NO20022532A NO20022532L (no) 1999-11-29 2002-05-28 Fremgangsmåte for fremstilling av nukleinsyrer fra en miljöpröve, de således oppnådde nukleinsyrer samt deres anvendelseved syntese av nye forbindelser
AU2005211587A AU2005211587A1 (en) 1999-11-29 2005-09-19 Method for obtaining nucleic acids from an environment sample, resulting nucleic acids and use in synthesis of novel compounds

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
FR9915032A FR2801609B1 (fr) 1999-11-29 1999-11-29 Procede d'obtention d'acides nucleiques a partir d'un echantillon de l'environnement, acides nucleiques ainsi obtenus et leur application a la synthese de nouveaux composes
FR99/15032 1999-11-29
US20980000P 2000-06-07 2000-06-07
US60/209,800 2000-06-07

Publications (2)

Publication Number Publication Date
WO2001040497A2 WO2001040497A2 (fr) 2001-06-07
WO2001040497A3 true WO2001040497A3 (fr) 2002-10-17

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2000/003311 WO2001040497A2 (fr) 1999-11-29 2000-11-27 Procede d'obtention d'acides nucleiques a partir d'un echantillon de l'environnement

Country Status (9)

Country Link
EP (1) EP1268764A2 (fr)
JP (1) JP2003520578A (fr)
KR (1) KR20020060242A (fr)
AU (2) AU781961B2 (fr)
BR (1) BR0015993A (fr)
CA (1) CA2393041A1 (fr)
IL (1) IL149846A0 (fr)
NO (1) NO20022532L (fr)
WO (1) WO2001040497A2 (fr)

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US7749366B2 (en) 2002-08-20 2010-07-06 B.R.A.I.N. Biotechnology Research And Information Network Ag Isolation and cloning of DNA from uncultivated organisms

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US7217510B2 (en) 2001-06-26 2007-05-15 Isis Pharmaceuticals, Inc. Methods for providing bacterial bioagent characterizing information
FR2834997B1 (fr) * 2002-01-21 2004-03-19 Evialis Procede de detection de champignons producteurs d'ochratoxine a ou de citrinine
EP1386966A1 (fr) 2002-07-24 2004-02-04 Libragen Méthode pour l'expresssion de l'ADN environmental dans des cellules hôtes
EP1905833B1 (fr) 2002-10-08 2015-08-12 Aventis Pharma S.A. Polypeptides impliqués dans la biosynthèse des spiramycines, séquences nucléotidiques codant ces polypeptides et leurs applications
CA2508726A1 (fr) 2002-12-06 2004-07-22 Isis Pharmaceuticals, Inc. Procedes d'identification rapide de pathogenes chez l'homme et les betes
US8057993B2 (en) 2003-04-26 2011-11-15 Ibis Biosciences, Inc. Methods for identification of coronaviruses
US8158354B2 (en) 2003-05-13 2012-04-17 Ibis Biosciences, Inc. Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
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US20120122103A1 (en) 2003-09-11 2012-05-17 Rangarajan Sampath Compositions for use in identification of bacteria
US8097416B2 (en) 2003-09-11 2012-01-17 Ibis Biosciences, Inc. Methods for identification of sepsis-causing bacteria
US8546082B2 (en) 2003-09-11 2013-10-01 Ibis Biosciences, Inc. Methods for identification of sepsis-causing bacteria
US8163895B2 (en) 2003-12-05 2012-04-24 Ibis Biosciences, Inc. Compositions for use in identification of orthopoxviruses
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CA2663029C (fr) 2006-09-14 2016-07-19 Ibis Biosciences, Inc. Procede d'amplification ciblee de genome entier pour l'identification d'agents pathogenes
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US9598724B2 (en) 2007-06-01 2017-03-21 Ibis Biosciences, Inc. Methods and compositions for multiple displacement amplification of nucleic acids
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7749366B2 (en) 2002-08-20 2010-07-06 B.R.A.I.N. Biotechnology Research And Information Network Ag Isolation and cloning of DNA from uncultivated organisms

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Publication number Publication date
JP2003520578A (ja) 2003-07-08
AU2179101A (en) 2001-06-12
NO20022532D0 (no) 2002-05-28
AU781961B2 (en) 2005-06-23
WO2001040497A2 (fr) 2001-06-07
BR0015993A (pt) 2002-08-06
EP1268764A2 (fr) 2003-01-02
AU2005211587A1 (en) 2005-10-13
NO20022532L (no) 2002-06-26
CA2393041A1 (fr) 2001-06-07
IL149846A0 (en) 2002-11-10
KR20020060242A (ko) 2002-07-16

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