WO1999014341A3 - Proteases extraites d'organismes gram positif - Google Patents

Proteases extraites d'organismes gram positif Download PDF

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Publication number
WO1999014341A3
WO1999014341A3 PCT/US1998/018677 US9818677W WO9914341A3 WO 1999014341 A3 WO1999014341 A3 WO 1999014341A3 US 9818677 W US9818677 W US 9818677W WO 9914341 A3 WO9914341 A3 WO 9914341A3
Authority
WO
WIPO (PCT)
Prior art keywords
present
proteases
gram
provides
positive organisms
Prior art date
Application number
PCT/US1998/018677
Other languages
English (en)
Other versions
WO1999014341A2 (fr
Inventor
David A Estell
Original Assignee
Genencor Int
Genencor Int Bv
David A Estell
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor Int, Genencor Int Bv, David A Estell filed Critical Genencor Int
Priority to US09/486,192 priority Critical patent/US6521440B1/en
Priority to AU93069/98A priority patent/AU9306998A/en
Priority to EP98945934A priority patent/EP1015606A2/fr
Publication of WO1999014341A2 publication Critical patent/WO1999014341A2/fr
Publication of WO1999014341A3 publication Critical patent/WO1999014341A3/fr
Priority to US10/328,459 priority patent/US6905868B2/en
Priority to US11/008,352 priority patent/US8101563B2/en
Priority to US11/008,331 priority patent/US7326531B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention concerne l'identification de nouvelles métalloprotéases (MP) dans des microorganismes Gram positif, ainsi que les séquences d'acides nucléiques et d'acides aminés des métalloprotéases extraites de Bacillus. L'invention concerne également des cellules hôtes présentant une mutation ou une délétion d'une partie ou de la totalité du gène codant les métalloprotéases, ainsi que des cellules hôtes comprenant aussi un acide nucléique codant des protéines hétérologues voulues, telles que des enzymes. L'invention concerne enfin des compositions de nettoyage comprenant une métalloprotéase de la présente invention.
PCT/US1998/018677 1997-09-15 1998-09-08 Proteases extraites d'organismes gram positif WO1999014341A2 (fr)

Priority Applications (6)

Application Number Priority Date Filing Date Title
US09/486,192 US6521440B1 (en) 1997-09-15 1998-09-08 Proteases from gram-positive organisms
AU93069/98A AU9306998A (en) 1997-09-15 1998-09-08 Proteases from gram-positive organisms
EP98945934A EP1015606A2 (fr) 1997-09-15 1998-09-08 Proteases extraites d'organismes gram positif
US10/328,459 US6905868B2 (en) 1997-09-15 2002-12-23 Proteases from gram-positive organisms
US11/008,352 US8101563B2 (en) 1997-09-15 2004-12-08 Proteases from gram-positive organisms
US11/008,331 US7326531B2 (en) 1997-09-15 2004-12-08 Proteases from gram-positive organisms

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9719637.2A GB9719637D0 (en) 1997-09-15 1997-09-15 Proteases from gram-positive organisms
GB9719637.2 1997-09-15

Related Child Applications (3)

Application Number Title Priority Date Filing Date
US09486192 A-371-Of-International 1998-09-08
US09/486,192 A-371-Of-International US6521440B1 (en) 1997-09-15 1998-09-08 Proteases from gram-positive organisms
US10/328,459 Division US6905868B2 (en) 1997-09-15 2002-12-23 Proteases from gram-positive organisms

Publications (2)

Publication Number Publication Date
WO1999014341A2 WO1999014341A2 (fr) 1999-03-25
WO1999014341A3 true WO1999014341A3 (fr) 1999-05-06

Family

ID=10819116

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/018677 WO1999014341A2 (fr) 1997-09-15 1998-09-08 Proteases extraites d'organismes gram positif

Country Status (4)

Country Link
EP (1) EP1015606A2 (fr)
AU (1) AU9306998A (fr)
GB (1) GB9719637D0 (fr)
WO (1) WO1999014341A2 (fr)

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JP2002541306A (ja) * 1999-04-01 2002-12-03 ザ、プロクター、エンド、ギャンブル、カンパニー 金属プロテアーゼを含有した洗剤組成物
US20160122738A1 (en) * 2013-05-29 2016-05-05 Danisco Us Inc. Novel metalloproteases
EP3080263B1 (fr) 2013-12-13 2019-07-03 Danisco US Inc. Sérines protéases du clade du bacillus gibsonii
DK3514230T3 (da) 2013-12-13 2021-11-15 Danisco Us Inc Serinproteaser af bacillus-arter
KR20160099629A (ko) 2013-12-16 2016-08-22 이 아이 듀폰 디 네모아 앤드 캄파니 점도 조절제로서의 폴리 알파-1,3-글루칸 에테르의 사용
EP3789407B1 (fr) 2013-12-18 2024-07-24 Nutrition & Biosciences USA 4, Inc. Éthers cationiques de poly(alpha-1,3-glucane)
CN105992796A (zh) 2014-02-14 2016-10-05 纳幕尔杜邦公司 用于粘度调节的聚-α-1,3-1,6-葡聚糖
WO2015138283A1 (fr) 2014-03-11 2015-09-17 E. I. Du Pont De Nemours And Company Poly(alpha-1,3-glucane) oxydé en tant qu'adjuvant pour détergent
DK3119884T3 (da) 2014-03-21 2019-10-14 Danisco Us Inc Serinproteaser af bacillus-arter
US9714403B2 (en) 2014-06-19 2017-07-25 E I Du Pont De Nemours And Company Compositions containing one or more poly alpha-1,3-glucan ether compounds
WO2015195777A1 (fr) 2014-06-19 2015-12-23 E. I. Du Pont De Nemours And Company Compositions contenant un ou plusieurs composés d'éther de poly alpha-1,3-glucane
WO2016061438A1 (fr) 2014-10-17 2016-04-21 Danisco Us Inc. Sérines protéases de l'espèce bacillus
DK3212662T3 (da) 2014-10-27 2020-07-20 Danisco Us Inc Serinproteaser
US20180010074A1 (en) 2014-10-27 2018-01-11 Danisco Us Inc. Serine proteases of bacillus species
DK3212781T3 (da) 2014-10-27 2019-12-16 Danisco Us Inc Serinproteaser
EP4403631A2 (fr) 2014-10-27 2024-07-24 Danisco US Inc. Sérine protéases
EP3957729A1 (fr) 2014-10-27 2022-02-23 Danisco US Inc. Protéases de sérine
AU2015369965B2 (en) 2014-12-23 2020-01-30 Nutrition & Biosciences USA 4, Inc. Enzymatically produced cellulose
WO2016183509A1 (fr) 2015-05-13 2016-11-17 Danisco Us Inc. Variants de protéase aprl-clade et leurs utilisations
EP4234693A3 (fr) 2015-06-17 2023-11-01 Danisco US Inc Protéases à sérines du clade du bacillus gibsonii
WO2017079751A1 (fr) 2015-11-05 2017-05-11 Danisco Us Inc Mannanases de paenibacillus sp.
JP7364330B2 (ja) 2015-11-05 2023-10-18 ダニスコ・ユーエス・インク パエニバチルス（Ｐａｅｎｉｂａｃｉｌｌｕｓ）属種及びバチルス（Ｂａｃｉｌｌｕｓ）属種のマンナナーゼ
WO2017083226A1 (fr) 2015-11-13 2017-05-18 E. I. Du Pont De Nemours And Company Compositions de fibre de glucane à utiliser dans l'entretien du linge et l'entretien de tissu
US10822574B2 (en) 2015-11-13 2020-11-03 Dupont Industrial Biosciences Usa, Llc Glucan fiber compositions for use in laundry care and fabric care
EP3374400B1 (fr) 2015-11-13 2022-04-13 Nutrition & Biosciences USA 4, Inc. Compositions de fibres de glucane utiles pour la lessive et l'entretien des tissus
EP3390625B1 (fr) 2015-12-18 2023-09-06 Danisco US Inc. Polypeptides ayant une activité endoglucanase et leurs utilisations
CN109477112A (zh) 2016-05-31 2019-03-15 丹尼斯科美国公司 蛋白酶变体及其用途
EP3472313B1 (fr) 2016-06-17 2022-08-31 Danisco US Inc. Variants de protéase et leurs utilisations
US20190264138A1 (en) 2016-11-07 2019-08-29 Danisco Us Inc. Laundry detergent composition
US20200392477A1 (en) 2016-12-21 2020-12-17 Danisco Us Inc. Protease variants and uses thereof
EP3559226B1 (fr) 2016-12-21 2023-01-04 Danisco US Inc. Sérine-protéases du clade du bacillus gibsonii
WO2018169750A1 (fr) 2017-03-15 2018-09-20 Danisco Us Inc Sérine protéases de type trypsine et leurs utilisations
EP3717643A1 (fr) 2017-11-29 2020-10-07 Danisco US Inc. Variants de subtilisine à stabilité améliorée
US20210363470A1 (en) 2018-06-19 2021-11-25 Danisco Us Inc Subtilisin variants
WO2019245704A1 (fr) 2018-06-19 2019-12-26 Danisco Us Inc Variantes de subtilisine
US20220033737A1 (en) 2018-09-27 2022-02-03 Danisco Us Inc Compositions for medical instrument cleaning
EP3887515A1 (fr) 2018-11-28 2021-10-06 Danisco US Inc. Variants de subtilisine à stabilité améliorée
EP3976776A1 (fr) 2019-05-24 2022-04-06 Danisco US Inc. Variants de subtilisine et procédés d'utilisation
US20220306968A1 (en) 2019-06-06 2022-09-29 Danisco Us Inc Methods and compositions for cleaning
US20240034960A1 (en) 2020-08-27 2024-02-01 Danisco Us Inc Enzymes and enzyme compositions for cleaning
EP4284906A1 (fr) 2021-01-29 2023-12-06 Danisco US Inc. Compositions pour le nettoyage et procédés associés
WO2023278297A1 (fr) 2021-06-30 2023-01-05 Danisco Us Inc Variants de lipases et leurs utilisations
CN117916354A (zh) 2021-09-03 2024-04-19 丹尼斯科美国公司 用于清洁的衣物洗涤组合物
WO2023114936A2 (fr) 2021-12-16 2023-06-22 Danisco Us Inc. Variants de subtilisine et procédés d'utilisation
WO2023114939A2 (fr) 2021-12-16 2023-06-22 Danisco Us Inc. Variants de subtilisine et procédés d'utilisation
WO2023114932A2 (fr) 2021-12-16 2023-06-22 Danisco Us Inc. Variants de subtilisine et procédés d'utilisation
WO2023168234A1 (fr) 2022-03-01 2023-09-07 Danisco Us Inc. Enzymes et compositions enzymatiques pour le nettoyage
WO2023250301A1 (fr) 2022-06-21 2023-12-28 Danisco Us Inc. Procédés et compositions de nettoyage comprenant un polypeptide ayant une activité de thermolysine
WO2024050346A1 (fr) 2022-09-02 2024-03-07 Danisco Us Inc. Compositions détergentes et procédés associés
WO2024050339A1 (fr) 2022-09-02 2024-03-07 Danisco Us Inc. Variants de mannanases et procédés d'utilisation
WO2024050343A1 (fr) 2022-09-02 2024-03-07 Danisco Us Inc. Variants de subtilisine et procédés associés
WO2024102698A1 (fr) 2022-11-09 2024-05-16 Danisco Us Inc. Variants de subtilisine et procédés d'utilisation

Citations (2)

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Publication number Priority date Publication date Assignee Title
EP0244042A1 (fr) * 1986-05-02 1987-11-04 Gist-Brocades N.V. Vecteurs de sélection du type "signal de sécrétion" pour la synthèse protéique extracellulaire dans les bacilles
EP0369817A2 (fr) * 1988-11-18 1990-05-23 Omnigene Bioproducts, Inc. Souches de bacillus

Family Cites Families (3)

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US4760025A (en) * 1984-05-29 1988-07-26 Genencor, Inc. Modified enzymes and methods for making same
US5346823A (en) * 1984-05-29 1994-09-13 Genencor, Inc. Subtilisin modifications to enhance oxidative stability
US5264366A (en) * 1984-05-29 1993-11-23 Genencor, Inc. Protease deficient bacillus

Patent Citations (2)

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Publication number Priority date Publication date Assignee Title
EP0244042A1 (fr) * 1986-05-02 1987-11-04 Gist-Brocades N.V. Vecteurs de sélection du type "signal de sécrétion" pour la synthèse protéique extracellulaire dans les bacilles
EP0369817A2 (fr) * 1988-11-18 1990-05-23 Omnigene Bioproducts, Inc. Souches de bacillus

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
EMBL/GENBANK DATABASES Accession no Y14083 Sequence reference BSY14083 27 June 1997 NOBACK M ET AL: "Bacillus subtilis yhfN gene" *
SMITH H ET AL: "Characterization of signal-sequence-coding regions selected from the B.subtilis chromosome", GENE, vol. 70, 1988, pages 351 - 361, XP002094691 *

Also Published As

Publication number Publication date
AU9306998A (en) 1999-04-05
WO1999014341A2 (fr) 1999-03-25
EP1015606A2 (fr) 2000-07-05
GB9719637D0 (en) 1997-11-19

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