WO1999014341A3 - Proteases from gram-positive organisms - Google Patents

Proteases from gram-positive organisms Download PDF

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Publication number
WO1999014341A3
WO1999014341A3 PCT/US1998/018677 US9818677W WO9914341A3 WO 1999014341 A3 WO1999014341 A3 WO 1999014341A3 US 9818677 W US9818677 W US 9818677W WO 9914341 A3 WO9914341 A3 WO 9914341A3
Authority
WO
WIPO (PCT)
Prior art keywords
present
proteases
gram
provides
positive organisms
Prior art date
Application number
PCT/US1998/018677
Other languages
French (fr)
Other versions
WO1999014341A2 (en
Inventor
David A Estell
Original Assignee
Genencor Int
Genencor Int Bv
David A Estell
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor Int, Genencor Int Bv, David A Estell filed Critical Genencor Int
Priority to US09/486,192 priority Critical patent/US6521440B1/en
Priority to AU93069/98A priority patent/AU9306998A/en
Priority to EP98945934A priority patent/EP1015606A2/en
Publication of WO1999014341A2 publication Critical patent/WO1999014341A2/en
Publication of WO1999014341A3 publication Critical patent/WO1999014341A3/en
Priority to US10/328,459 priority patent/US6905868B2/en
Priority to US11/008,331 priority patent/US7326531B2/en
Priority to US11/008,352 priority patent/US8101563B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to the identification of novel metallo-proteases (MP) in Gram-positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for Bacillus MP. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding MP. The present invention also provides host cells further comprising nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides cleaning compositions comprising an MP of the present invention.
PCT/US1998/018677 1997-09-15 1998-09-08 Proteases from gram-positive organisms WO1999014341A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
US09/486,192 US6521440B1 (en) 1997-09-15 1998-09-08 Proteases from gram-positive organisms
AU93069/98A AU9306998A (en) 1997-09-15 1998-09-08 Proteases from gram-positive organisms
EP98945934A EP1015606A2 (en) 1997-09-15 1998-09-08 Proteases from gram-positive organisms
US10/328,459 US6905868B2 (en) 1997-09-15 2002-12-23 Proteases from gram-positive organisms
US11/008,331 US7326531B2 (en) 1997-09-15 2004-12-08 Proteases from gram-positive organisms
US11/008,352 US8101563B2 (en) 1997-09-15 2004-12-08 Proteases from gram-positive organisms

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9719637.2 1997-09-15
GBGB9719637.2A GB9719637D0 (en) 1997-09-15 1997-09-15 Proteases from gram-positive organisms

Related Child Applications (3)

Application Number Title Priority Date Filing Date
US09/486,192 A-371-Of-International US6521440B1 (en) 1997-09-15 1998-09-08 Proteases from gram-positive organisms
US09486192 A-371-Of-International 1998-09-08
US10/328,459 Division US6905868B2 (en) 1997-09-15 2002-12-23 Proteases from gram-positive organisms

Publications (2)

Publication Number Publication Date
WO1999014341A2 WO1999014341A2 (en) 1999-03-25
WO1999014341A3 true WO1999014341A3 (en) 1999-05-06

Family

ID=10819116

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/018677 WO1999014341A2 (en) 1997-09-15 1998-09-08 Proteases from gram-positive organisms

Country Status (4)

Country Link
EP (1) EP1015606A2 (en)
AU (1) AU9306998A (en)
GB (1) GB9719637D0 (en)
WO (1) WO1999014341A2 (en)

Families Citing this family (52)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1171564A1 (en) * 1999-04-01 2002-01-16 The Procter & Gamble Company A detergent composition containing a metallo-protease
EP4159854A1 (en) * 2013-05-29 2023-04-05 Danisco US Inc Novel metalloproteases
TR201906371T4 (en) 2013-12-13 2019-05-21 Danisco Inc Serine proteases of Bacillus species.
CN106029881B (en) 2013-12-13 2023-01-10 丹尼斯科美国公司 Serine proteases of the bacillus gibsonii-clade
EP4163305A1 (en) 2013-12-16 2023-04-12 Nutrition & Biosciences USA 4, Inc. Use of poly alpha-1,3-glucan ethers as viscosity modifiers
MX2016007920A (en) 2013-12-18 2016-09-13 Du Pont Cationic poly alpha-1,3-glucan ethers.
EP3105256A1 (en) 2014-02-14 2016-12-21 E. I. du Pont de Nemours and Company Poly-alpha-1,3-1,6-glucans for viscosity modification
WO2015138283A1 (en) 2014-03-11 2015-09-17 E. I. Du Pont De Nemours And Company Oxidized poly alpha-1,3-glucan as detergent builder
EP3119884B1 (en) 2014-03-21 2019-07-10 Danisco US Inc. Serine proteases of bacillus species
US9771548B2 (en) 2014-06-19 2017-09-26 E I Du Pont De Nemours And Company Compositions containing one or more poly alpha-1,3-glucan ether compounds
US9714403B2 (en) 2014-06-19 2017-07-25 E I Du Pont De Nemours And Company Compositions containing one or more poly alpha-1,3-glucan ether compounds
DK3207129T3 (en) 2014-10-17 2020-02-24 Danisco Us Inc SERIN PROTEAS OF THE BACILLUS ART
EP3212782B1 (en) 2014-10-27 2019-04-17 Danisco US Inc. Serine proteases
WO2016069548A2 (en) 2014-10-27 2016-05-06 Danisco Us Inc. Serine proteases
CN107148472A (en) 2014-10-27 2017-09-08 丹尼斯科美国公司 The serine protease of Bacillus spec
WO2016069552A1 (en) 2014-10-27 2016-05-06 Danisco Us Inc. Serine proteases
WO2016069544A1 (en) 2014-10-27 2016-05-06 Danisco Us Inc. Serine proteases
CA2969242A1 (en) 2014-12-23 2016-06-30 E.I. Du Pont De Nemours And Company Enzymatically produced cellulose
JP7274819B2 (en) 2015-05-13 2023-05-17 ダニスコ・ユーエス・インク AprL-CLADE protease variants and uses thereof
EP4234693A3 (en) 2015-06-17 2023-11-01 Danisco US Inc Bacillus gibsonii-clade serine proteases
JP7364330B2 (en) 2015-11-05 2023-10-18 ダニスコ・ユーエス・インク Mannanase of Paenibacillus sp. and Bacillus sp.
EP3371308B1 (en) 2015-11-05 2022-05-11 Danisco US Inc. Paenibacillus sp. mannanases
US10844324B2 (en) 2015-11-13 2020-11-24 Dupont Industrial Biosciences Usa, Llc Glucan fiber compositions for use in laundry care and fabric care
JP2019504932A (en) 2015-11-13 2019-02-21 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ Glucan fiber composition for use in laundry and textile care
US10876074B2 (en) 2015-11-13 2020-12-29 Dupont Industrial Biosciences Usa, Llc Glucan fiber compositions for use in laundry care and fabric care
CN108699543B (en) 2015-12-18 2023-07-14 丹尼斯科美国公司 Polypeptides having endoglucanase activity and uses thereof
US11661567B2 (en) 2016-05-31 2023-05-30 Danisco Us Inc. Protease variants and uses thereof
WO2017219011A1 (en) 2016-06-17 2017-12-21 Danisco Us Inc Protease variants and uses thereof
WO2018085524A2 (en) 2016-11-07 2018-05-11 Danisco Us Inc Laundry detergent composition
EP3559226B1 (en) 2016-12-21 2023-01-04 Danisco US Inc. Bacillus gibsonii-clade serine proteases
CN110312795A (en) 2016-12-21 2019-10-08 丹尼斯科美国公司 Ease variants and application thereof
EP3583210B1 (en) 2017-03-15 2021-07-07 Danisco US Inc. Trypsin-like serine proteases and uses thereof
US20200354708A1 (en) 2017-11-29 2020-11-12 Danisco Us Inc. Subtilisin variants having improved stability
WO2019245704A1 (en) 2018-06-19 2019-12-26 Danisco Us Inc Subtilisin variants
WO2019245705A1 (en) 2018-06-19 2019-12-26 Danisco Us Inc Subtilisin variants
WO2020068486A1 (en) 2018-09-27 2020-04-02 Danisco Us Inc Compositions for medical instrument cleaning
EP3887515A1 (en) 2018-11-28 2021-10-06 Danisco US Inc. Subtilisin variants having improved stability
EP3976776A1 (en) 2019-05-24 2022-04-06 Danisco US Inc. Subtilisin variants and methods of use
EP3980517A1 (en) 2019-06-06 2022-04-13 Danisco US Inc. Methods and compositions for cleaning
EP4204553A1 (en) 2020-08-27 2023-07-05 Danisco US Inc. Enzymes and enzyme compositions for cleaning
WO2022165107A1 (en) 2021-01-29 2022-08-04 Danisco Us Inc Compositions for cleaning and methods related thereto
WO2023278297A1 (en) 2021-06-30 2023-01-05 Danisco Us Inc Variant lipases and uses thereof
CN117916354A (en) 2021-09-03 2024-04-19 丹尼斯科美国公司 Laundry compositions for cleaning
WO2023114939A2 (en) 2021-12-16 2023-06-22 Danisco Us Inc. Subtilisin variants and methods of use
WO2023114932A2 (en) 2021-12-16 2023-06-22 Danisco Us Inc. Subtilisin variants and methods of use
WO2023114936A2 (en) 2021-12-16 2023-06-22 Danisco Us Inc. Subtilisin variants and methods of use
WO2023168234A1 (en) 2022-03-01 2023-09-07 Danisco Us Inc. Enzymes and enzyme compositions for cleaning
WO2023250301A1 (en) 2022-06-21 2023-12-28 Danisco Us Inc. Methods and compositions for cleaning comprising a polypeptide having thermolysin activity
WO2024050343A1 (en) 2022-09-02 2024-03-07 Danisco Us Inc. Subtilisin variants and methods related thereto
WO2024050339A1 (en) 2022-09-02 2024-03-07 Danisco Us Inc. Mannanase variants and methods of use
WO2024050346A1 (en) 2022-09-02 2024-03-07 Danisco Us Inc. Detergent compositions and methods related thereto
WO2024102698A1 (en) 2022-11-09 2024-05-16 Danisco Us Inc. Subtilisin variants and methods of use

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0244042A1 (en) * 1986-05-02 1987-11-04 Gist-Brocades N.V. Secretory signal selection vectors for extracellular protein synthesis in bacilli
EP0369817A2 (en) * 1988-11-18 1990-05-23 Omnigene Bioproducts, Inc. Bacillus strains

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4760025A (en) * 1984-05-29 1988-07-26 Genencor, Inc. Modified enzymes and methods for making same
US5264366A (en) * 1984-05-29 1993-11-23 Genencor, Inc. Protease deficient bacillus
US5346823A (en) * 1984-05-29 1994-09-13 Genencor, Inc. Subtilisin modifications to enhance oxidative stability

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0244042A1 (en) * 1986-05-02 1987-11-04 Gist-Brocades N.V. Secretory signal selection vectors for extracellular protein synthesis in bacilli
EP0369817A2 (en) * 1988-11-18 1990-05-23 Omnigene Bioproducts, Inc. Bacillus strains

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
EMBL/GENBANK DATABASES Accession no Y14083 Sequence reference BSY14083 27 June 1997 NOBACK M ET AL: "Bacillus subtilis yhfN gene" *
SMITH H ET AL: "Characterization of signal-sequence-coding regions selected from the B.subtilis chromosome", GENE, vol. 70, 1988, pages 351 - 361, XP002094691 *

Also Published As

Publication number Publication date
AU9306998A (en) 1999-04-05
WO1999014341A2 (en) 1999-03-25
GB9719637D0 (en) 1997-11-19
EP1015606A2 (en) 2000-07-05

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