WO1999003984A3 - Proteases from gram-positive organisms - Google Patents

Proteases from gram-positive organisms Download PDF

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Publication number
WO1999003984A3
WO1999003984A3 PCT/US1998/014647 US9814647W WO9903984A3 WO 1999003984 A3 WO1999003984 A3 WO 1999003984A3 US 9814647 W US9814647 W US 9814647W WO 9903984 A3 WO9903984 A3 WO 9903984A3
Authority
WO
WIPO (PCT)
Prior art keywords
present
gram
provides
proteases
nucleic acid
Prior art date
Application number
PCT/US1998/014647
Other languages
French (fr)
Other versions
WO1999003984A2 (en
Inventor
David A Estell
Original Assignee
Genencor Int
Genencor Int Bv
David A Estell
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to US09/462,845 priority Critical patent/US6723550B1/en
Priority to AU84870/98A priority patent/AU8487098A/en
Priority to JP2000503190A priority patent/JP2001510037A/en
Priority to EP98935681A priority patent/EP0991754A2/en
Application filed by Genencor Int, Genencor Int Bv, David A Estell filed Critical Genencor Int
Publication of WO1999003984A2 publication Critical patent/WO1999003984A2/en
Publication of WO1999003984A3 publication Critical patent/WO1999003984A3/en
Priority to US10/401,436 priority patent/US6911333B2/en
Priority to US10/402,067 priority patent/US6881562B2/en
Priority to US10/401,437 priority patent/US6849440B2/en
Priority to US11/014,432 priority patent/US7329527B2/en
Priority to US11/014,051 priority patent/US7329525B2/en
Priority to US11/014,364 priority patent/US7316920B2/en
Priority to US11/013,991 priority patent/US20050101001A1/en
Priority to US11/014,339 priority patent/US7329526B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38663—Stabilised liquid enzyme compositions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)

Abstract

The present invention relates to the identification of novel serine proteases in Gram-positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for the Bacillus subtilis serine proteases SP1, SP2, SP3, SP4 and SP5. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding SP1, SP2, SP3, SP4 and SP5. The present invention also provides host cells further comprising nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides a cleaning composition comprising a serine protease of the present invention.
PCT/US1998/014647 1997-07-15 1998-07-14 Proteases from gram-positive organisms WO1999003984A2 (en)

Priority Applications (12)

Application Number Priority Date Filing Date Title
US09/462,845 US6723550B1 (en) 1997-07-15 1998-07-14 Proteases from gram-positive organisms
AU84870/98A AU8487098A (en) 1997-07-15 1998-07-14 Proteases from gram-positive organisms
JP2000503190A JP2001510037A (en) 1997-07-15 1998-07-14 Proteases from Gram-positive microorganisms
EP98935681A EP0991754A2 (en) 1997-07-15 1998-07-14 Proteases from gram-positive organisms
US10/401,437 US6849440B2 (en) 1997-07-15 2003-03-26 Proteases from gram-positive organisms
US10/401,436 US6911333B2 (en) 1997-07-15 2003-03-26 Proteases from gram-positive organisms
US10/402,067 US6881562B2 (en) 1997-07-15 2003-03-26 Proteases from gram-positive organisms
US11/014,432 US7329527B2 (en) 1997-07-15 2004-12-16 Serine proteases from gram-positive microorganisms
US11/014,051 US7329525B2 (en) 1997-07-15 2004-12-16 Serine proteases from gram-positive microorganisms
US11/014,364 US7316920B2 (en) 1997-07-15 2004-12-16 Serine proteases from gram-positive microorganisms
US11/013,991 US20050101001A1 (en) 1997-07-15 2004-12-16 Proteases from gram-position organisms
US11/014,339 US7329526B2 (en) 1997-07-15 2004-12-16 Serine proteases from-gram-positive microorganisms

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP97305232 1997-07-15
EP97305232.7 1997-07-15

Related Child Applications (6)

Application Number Title Priority Date Filing Date
US09462845 A-371-Of-International 1998-07-14
US09/462,845 A-371-Of-International US6723550B1 (en) 1997-07-15 1998-07-14 Proteases from gram-positive organisms
US10/402,312 Division US6833261B2 (en) 1997-07-15 2003-03-26 Proteases from gram-positive organisms
US10/402,067 Division US6881562B2 (en) 1997-07-15 2003-03-26 Proteases from gram-positive organisms
US10/401,437 Division US6849440B2 (en) 1997-07-15 2003-03-26 Proteases from gram-positive organisms
US10/401,436 Division US6911333B2 (en) 1997-07-15 2003-03-26 Proteases from gram-positive organisms

Publications (2)

Publication Number Publication Date
WO1999003984A2 WO1999003984A2 (en) 1999-01-28
WO1999003984A3 true WO1999003984A3 (en) 1999-04-08

Family

ID=8229415

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/014647 WO1999003984A2 (en) 1997-07-15 1998-07-14 Proteases from gram-positive organisms

Country Status (4)

Country Link
EP (1) EP0991754A2 (en)
JP (1) JP2001510037A (en)
AU (1) AU8487098A (en)
WO (1) WO1999003984A2 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9724629D0 (en) * 1997-11-20 1998-01-21 Genencor Int Bv Alpha/beta hydrolase-fold enzymes
US8124399B2 (en) 2002-03-29 2012-02-28 Danisco Us Inc. Enhanced protein expression in Bacillus
US8288136B2 (en) 2005-12-13 2012-10-16 E. I. Du Pont De Nemours And Company Production of peracids using an enzyme having perhydrolysis activity
US7951566B2 (en) 2005-12-13 2011-05-31 E.I. Du Pont De Nemours And Company Production of peracids using an enzyme having perhydrolysis activity
US8518675B2 (en) 2005-12-13 2013-08-27 E. I. Du Pont De Nemours And Company Production of peracids using an enzyme having perhydrolysis activity
US7723083B2 (en) 2005-12-13 2010-05-25 E.I. Du Pont De Nemours And Company Production of peracids using an enzyme having perhydrolysis activity
US7964378B2 (en) 2005-12-13 2011-06-21 E.I. Du Pont De Nemours And Company Production of peracids using an enzyme having perhydrolysis activity
ATE557085T1 (en) 2008-08-13 2012-05-15 Du Pont CONTROLLING THE ENZYMATIC PRODUCTION OF PERACIDS
ES2735230T3 (en) 2008-10-03 2019-12-17 Du Pont Stabilization of perhydrolases with excipients
WO2011017095A2 (en) 2009-07-27 2011-02-10 E. I. Du Pont De Nemours And Company Enzymatic in situ preparation of peracid-based removable antimicrobial coating compositions and methods of use
US8222012B2 (en) 2009-10-01 2012-07-17 E. I. Du Pont De Nemours And Company Perhydrolase for enzymatic peracid production
CN102952787B (en) * 2012-10-22 2014-07-16 南京工业大学 Acetylxylan esterase and application thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0369817A2 (en) * 1988-11-18 1990-05-23 Omnigene Bioproducts, Inc. Bacillus strains

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0369817A2 (en) * 1988-11-18 1990-05-23 Omnigene Bioproducts, Inc. Bacillus strains

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
EMBL/GENBANK/DDBJ DATABASES Accession no O32120, Sequence reference O32120 1 January 1998 *
EMBL/GENBANK/DDBJ DATABASES Accession no P70948, Sequence reference P70948 1 February 1997 LEVINE A ET AL: "YITV" *
KUNST F. ET AL: "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis", NATURE, vol. 390, 20 November 1997 (1997-11-20), pages 249 - 256, XP002080813 *
LEVINE A ET AL: "A 10.3 kbp segment from nprB to argJ at the 102 region of the Bacillus subtilis chromosome", MICROBIOLOGY, vol. 143, January 1997 (1997-01-01), pages 175 - 177, XP002080812 *

Also Published As

Publication number Publication date
WO1999003984A2 (en) 1999-01-28
EP0991754A2 (en) 2000-04-12
AU8487098A (en) 1999-02-10
JP2001510037A (en) 2001-07-31

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