Classifications

• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D333/00—Heterocyclic compounds containing five-membered rings having one sulfur atom as the only ring hetero atom
  • C07D333/02—Heterocyclic compounds containing five-membered rings having one sulfur atom as the only ring hetero atom not condensed with other rings
  • C07D333/04—Heterocyclic compounds containing five-membered rings having one sulfur atom as the only ring hetero atom not condensed with other rings not substituted on the ring sulphur atom
  • C07D333/06—Heterocyclic compounds containing five-membered rings having one sulfur atom as the only ring hetero atom not condensed with other rings not substituted on the ring sulphur atom with only hydrogen atoms, hydrocarbon or substituted hydrocarbon radicals, directly attached to the ring carbon atoms
  • C07D333/22—Radicals substituted by doubly bound hetero atoms, or by two hetero atoms other than halogen singly bound to the same carbon atom
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D333/00—Heterocyclic compounds containing five-membered rings having one sulfur atom as the only ring hetero atom
  • C07D333/02—Heterocyclic compounds containing five-membered rings having one sulfur atom as the only ring hetero atom not condensed with other rings
  • C07D333/04—Heterocyclic compounds containing five-membered rings having one sulfur atom as the only ring hetero atom not condensed with other rings not substituted on the ring sulphur atom
  • C07D333/06—Heterocyclic compounds containing five-membered rings having one sulfur atom as the only ring hetero atom not condensed with other rings not substituted on the ring sulphur atom with only hydrogen atoms, hydrocarbon or substituted hydrocarbon radicals, directly attached to the ring carbon atoms
  • C07D333/14—Radicals substituted by singly bound hetero atoms other than halogen
  • C07D333/16—Radicals substituted by singly bound hetero atoms other than halogen by oxygen atoms
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
  • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms

Definitions

• the present invention relates to a process for preparing optically active alkanols by enzymically reducing the corresponding ketones, in particular to preparing (1S)-3-methylamino-1-(2-thienyl)propan-1-ol and (1S)-3-chloro-1-(2-thienyl)propan-1-ol.
• Duloxetine alcohol is a building block in Duloxetine synthesis.
• Duloxetine® is a drug which is currently at the approval stage and is intended to be used in the depression and incontinence fields of indication.
• EP-B-0273658 describes a process for preparing the base corresponding to Duloxetine by reacting 2-acetylthiophene with formaldehyde and dimethylamine in a Mannich reaction, reducing the keto group of the Mannich base thus obtained to give the racemic (S)-3-N,N-dimethylamino-1-(thien-2-yl)propan-1-ol, etherifying the alcohol function with naphthyl fluoride and finally converting the dimethylamino group to a methylamino function.
• the desired naphthyl ether enantiomer is obtained using chiral starting materials or by resolution of the racemates at the stage of the final product, for example via the salts using optically active acids or chromatography on a chiral stationary phase.
• U.S. Pat. No. 5,362,886 describes an analogous process which comprises adding S-mandelic acid to the racemic propanol obtained after reduction of the keto group.
• the S-enantiomer of the alcohol, obtained in this process, is used in the subsequent reaction stages.
• EP-A-0457559 likewise describes a process analogous to that of EP-B-0273658.
• the keto group of the Mannich base is reduced to the S-enantiomer form of the alcohol by using the asymmetric reduction system LAH-Icb (lithium aluminum hydride [(2R,2S)-( ⁇ )-4-dimethylamino-1,2-diphenyl-3-methyl-2-butanol]).
• LAH-Icb lithium aluminum hydride [(2R,2S)-( ⁇ )-4-dimethylamino-1,2-diphenyl-3-methyl-2-butanol]
• the alcohol obtained in this way is converted, by successively reacting with sodium iodide and subsequently with methylamine, into (S)-3-methylamino-1-(thien-2-yl)propan-1-ol. Subsequent successive reaction with sodium hydride, 1-fluoronaphthalene and hydrogen chloride produces Duloxetine in the form of the hydrochloride.
• the present invention firstly relates to a method for preparing optically active alkanols of the formula I
• Enzymes (E) which are particularly suitable according to the invention are the enzymes of the families of aldo-keto reductases of the aldo-keto reductase superfamily (K. M. Bohren, B. Bullock, B. Wermuth and K. H. Gabbay J. Biol. Chem. 1989, 264, 9547-9551) and the short chain alcohol dehydrogenases/reductases [SDR].
• SDR short chain alcohol dehydrogenases/reductases
• the latter group of enzymes is described in detail, for example, in H. Jörnvall, B. Persson, M. Krook, S. Atrian, R. Gonzalez-Duarte, J. Jeffery and D. Ghosh, Biochemistry, 1995, 34, pp. 6003-6013 or U. Oppermann, C.
• the short chain alcohol dehydrogenases are particularly suitable.
• a particularly suitable embodiment of the invention is the use of enzymes (E) in the abovementioned process, with E having a polypeptide sequence
• the process serves to prepare derivatives of the 1-(2-thienyl)-(S)propanol of the formula III
• this compound is enzymically reduced to give the compound of the formula III and the essentially enantiomerically pure product formed is isolated.
• an enzyme with dehydrogenase activity which may be prepared from microorganisms of the genera Azoarcus, Azonexus, Azospira, Azovibrio, Dechloromonas, Ferribacterium, Petrobacter, Propionivibrio, Quadricoccus, Rhodocyclus, Sterolibacterium, Thauera and Zoogloea.
• the Azoarcus sp EbN1 phenylethanol dehydrogenase may be included in the short-chain alcohol dehydrogenases/reductases [SDR].
• Said group of enzymes is described in detail, for example, in H. Jörnvall, B. Persson, M. Krook, S. Atrian, R. Gonzalez-Duarte, J. Jeffery and D. Ghosh, Biochemistry, 1995, 34, pp. 6003-6013 or U. Oppermann, C. Filling, M. Hult, N. Shafqat, X. Q. Wu, M. Lindh, J. Shafqat, E. Nordling, Y. Kallberg, B. Persson and H.
• Azoarcus species are Azoarcus anaerobius, Azoarcus buckelii, Azoarcus communis, Azoarcus evansii, Azoarcus indigens, Azoarcus toluclasticus, Azoarcus tolulyticus, Azoarcus toluvorans, Azoarcus sp., Azoarcus sp. 22Lin, Azoarcus sp. BH72, Azoarcus sp. CC-11, Azoarcus sp. CIB, Azoarcus sp. CR23, Azoarcus sp. EB1, Azoarcus sp. EbN1, Azoarcus sp.
• the enzyme with dehydrogenase activity is selected from among enzymes which comprise an amino acid sequence according to SEQ ID NO: 2 or a sequence derived therefrom in which up to 25%, preferably up to 20%, particularly preferably up to 15%, in particular up to 10, 9, 8, 7, 6, 5, 4, 3, 2, 1%, of the amino acid residues have been altered by a deletion, a substitution, an insertion or a combination of deletion, substitution and insertion, with the polypeptide sequences altered compared to SEQ ID NO: 2 retaining at least 50%, preferably 65%, particularly preferably 80%, in particular more than 90%, of the enzymic activity of SEQ ID NO:2.
• enzymic activity of SEQ ID NO:2 is intended to mean the ability to reduce the ketones of the formula (IV), where R 1 ⁇ Cl, in an enantioselective manner to give the (S) alcohol having the general formula (III).
• the precise conditions for determining the enzymic activity are stated in examples 3 and 4.
• the process of the invention is carried out with the addition of reduction equivalents, in particular NADH or NADPH.
• reduction equivalents consumed during the reaction are regenerated by using a sacrificial alcohol, preferably isopropanol, 2-butanol, 2-pentanol, 2-hexanol, 3-hexanol, which is oxidized to the corresponding sacrificial ketone by the enzyme (E) under the reaction conditions.
• the added sacrificial alcohol is used not only for regenerating the reduction equivalents consumed but also as a cosolvent.
• Preference is given to working in a liquid 2-phase system, with one phase consisting of water or a water-miscible solvent and the other phase consisting of the sacrificial alcohol.
• Preference is given to using 2-pentanol as sacrificial alcohol.
• the reduction equivalents are used preferably in an amount of from 0.001 to 100 mmol, particularly preferably from 0.01 to 1 mmol, reduction equivalents per mole of alkanone (II) used.
• the process of the invention comprises removing, at least partially, the sacrificial ketone produced by oxidation of the sacrificial alcohol during regeneration of the reduction equivalents consumed from the reaction mixture.
• Preference is given to removing the sacrificial ketone formed completely from the reaction medium. This may be accomplished in various ways, for example by means of selective membranes or by extraction or distillation processes.
• Preference is given to removing the ketone by means of distillation. Removal of the ketone by distillation is usually accompanied by part of the sacrificial alcohol and sometimes additionally also parts of the aqueous phase also being removed. These distillatory losses are usually balanced by introducing further sacrificial alcohols and, if appropriate, further water.
• the rates of distillation are in a range from 0.02%/min to 2%/min, preferably from 0.05%/min to 1%/min, based on the reaction volume.
• the reactor jacket temperatures are between 5-70 Kelvin, preferably between 10-40 Kelvin, above the internal reactor temperature.
• the distillation is carried out particularly well in a pressure range of 1-500 mbar, preferably 10-200 mbar.
• a preferred embodiment comprises reacting the compound of the general formula II, such as, for example, the formula IV in the presence of a microorganism selected from among bacteria of the families Enterobacteriaceae, Pseudomonadaceae, Rhizobiaceae, Lactobacillaceae, Streptomycetaceae, Rhodococcaceae and Nocardiaceae.
• Said microorganism may be in particular a recombinant microorganism which has been transformed with a nucleic acid construct coding for an enzyme with dehydrogenase activity as defined above.
• the invention relates in particular to
• the invention moreover relates to coding nucleic acid sequences comprising the sequence coding for a polypeptide as defined above.
• the invention furthermore relates to expression cassettes comprising a coding nucleic acid sequence as defined above, which is operatively linked to at least one regulatory nucleic acid sequence.
• the invention further relates to recombinant vectors comprising at least one such expression cassette.
• the invention also relates to prokaryotic or eukaryotic hosts which have been transformed with at least one vector of the invention.
• the invention relates to the use of an enzyme with dehydrogenase activity as defined above or of a microorganism producing said enzyme for preparing compounds of the formulae I or III, and to further processing thereof, for example for preparing Duloxetine (formula VIII)
• Halogen is fluoro, chloro, bromo or iodo, in particular fluoro or chloro.
• “Lower alkyl” are straight-chain or branched alkyl radicals 1 to 6 carbon atoms, such as methyl, ethyl, isopropyl or n-propyl, n-, iso-, sec- or tert-butyl, n-pentyl or 2-methylbutyl, n-hexyl, 2-methylpentyl, 3-methylpentyl, 2-ethylbutyl.
• “Lower alkenyl” are the mono- or poly-, preferably mono- or di-, unsaturated analogs of the abovementioned alkyl radicals having 2 to 6 carbon atoms, it being possible for the double bond to be in any position of the carbon chain.
• “Lower alkoxy” are the oxygen-terminated analogs of the alkyl radicals above.
• Aryl is a mono- or polynuclear, preferably mono- or dinuclear, optionally substituted aromatic radical, in particular phenyl or naphthyl bound via any ring position, such as 1- or 2-naphthyl. These aryl radicals may, if appropriate, bear 1 or 2 identical or different substituents selected from among halogen, lower alkyl, lower alkoxy as defined above or trifluoromethyl.
• Alkanols accessible according to the invention by enzymic catalysis are those of the formula (I) above in which
• alkanones of the above formula II which are used for enzymic synthesis are compounds known per se and accessible by applying well-known organic syntheses (cf. e.g. EP-A-0 273 658).
• n is preferably 0, 1 or 2, in particular 1.
• Cyc examples are in particular mono- or dinuclear, preferably mononuclear, groups having up to 4, preferably 1 or 2, identical or different ring heteroatoms selected from among O, N and S:
• carbo- or heterocyclic rings comprise in particular from 3 to 12, preferably 4, 5 or 6, ring carbon atoms.
• Examples which may be mentioned are cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, the mono- or polyunsaturated analogs thereof, such as cyclobutenyl, cyclopentenyl, cyclohexenyl, cycloheptenyl, cyclohexadienyl, cycloheptadienyl; and also 5- to 7-membered saturated or mono- or polyunsaturated heterocyclic radicals having 1 to 4 heteroatoms selected from among O, N and S, it being possible for the heterocycle, if appropriate, to be fused to another heterocycle or carbocycle.
• heterocyclic radicals derived from pyrrolidine, tetrahydrofuran, piperidine, morpholine, pyrrole, furan, thiophene, pyrazole, imidazole, oxazole, thiazole, pyridine, pyran, pyrimidine, pyridazine, pyrazine, cumarone, indole and quinoline.
• radicals Cyc may be bound via any ring position, preferably via a ring carbon atom, to the alkanone or the alkanol.
• Cyc radicals are 2-thienyl, 3-thienyl; 2-furanyl, 3-furanyl; 2-pyridyl, 3-pyridyl or 4-pyridyl; 2-thiazolyl, 4-thiazolyl or 5-thiazolyl; 4-methyl-2-thienyl, 3-ethyl-2-thienyl, 2-methyl-3-thienyl, 4-propyl-3-thienyl, 5-n-butyl-2-thienyl, 4-methyl-3-thienyl, 3-methyl-2-thienyl; 3-chloro-2-thienyl, 4-bromo-3-thienyl, 2-iodo-3-thienyl, 5-iodo-3-thienyl, 4-fluoro-2-thienyl, 2-bromo-3-thienyl, and 4-chloro-2-thienyl.
• the radicals Cyc may furthermore be mono- or poly-, such as, for example, mono- or di-, substituted.
• the substituents are preferably located on a ring carbon atom. Examples of suitable substituents are halogen, lower alkyl, lower alkenyl, lower alkoxy, —OH, —SH, —NO 2 or NR 2 R 3 , where R 2 and R 3 are as defined above, preferably halogen or lower alkyl.
• R 1 is in particular halogen, NR 2 R 3 or NR 2 R 3 R 4+ X ⁇ , where R 2 , R 3 and, respectively, R 2 , R 3 and R 4 are independently of one another hydrogen or a lower alkyl or lower alkoxy radical and X ⁇ is a counterion, preference being given to any of the radicals R 2 , R 3 and R 4 being hydrogen.
• suitable counterions are acid anions, as are produced, for example, when preparing an acid addition salt. Examples thereof are mentioned, for example, in EP-A-0 273 658 to which reference is made hereby.
• radicals R 1 are in particular fluoro or chloro, and NR 2 R 3 in which R 2 and R 3 are identical or different and are hydrogen or methyl, ethyl or n-propyl; R 1 is particularly preferably chloro or —NHmethyl.
• Preferred enzymes with dehydrogenase activity comprise an amino acid sequence according to SEQ ID NO: 2.
• the invention likewise comprises “functional equivalents” of the specifically disclosed enzymes with dehydrogenase activity and the use of these in the processes of the invention.
• “functional equivalents” or analogs of the specifically disclosed enzymes are polypeptides which differ from these enzymes but which still possess the desired biological activity such as substrate specificity, for example.
• “functional equivalents” mean, for example, enzymes which reduce 3-chloro-1-(thien-2-yl)propan-1-one to the corresponding S-alcohol and which have at least 50%, preferably 60%, particularly preferably 75%, very particularly preferably 90%, of the activity of an enzyme having the amino acid sequence listed in SEQ ID NO:2.
• Functional equivalents are also preferably stable between pH 4 and 10 and advantageously possess a pH optimum between pH 5 and 8 and also a temperature optimum in the range from 20° C. to 80° C.
• “functional equivalents” also mean, in particular, mutants which have an amino acid other than the specifically mentioned amino acid in at least one sequence position of the abovementioned amino acid sequences but nevertheless possess one of the abovementioned biological activities. “Functional equivalents” thus comprise the mutants obtainable by one or more amino acid additions, amino acid substitutions, amino acid deletions and/or amino acid inversions, it being possible for said alterations to occur in any sequence position as long as they result in a mutant having the property profile of the invention. Functional equivalence also exists, in particular, when the reactivity patterns of the mutant and the unaltered polypeptide agree qualitatively, i.e. the same substrates are, for example, converted at different rates.
• “functional equivalents” also mean, in particular, mutants which have an amino acid other than the specifically mentioned amino acid in at least one sequence position of the abovementioned amino acid sequences but nevertheless possess one of the abovementioned biological activities.
• “Functional equivalence” thus comprise the mutants obtainable by one or more amino acid additions, amino acid substitutions, amino acid deletions and/or amino acid inversions, it being possible for said alterations to occur in any sequence position as long as they result in a mutant having the property profile of the invention.
• Functional equivalence also exists, in particular, when the reactivity patterns of the mutant and the unaltered polypeptide agree qualitatively, i.e. the same substrates are, for example, converted at different rates.
• precursors are natural or synthetic precursors of the polypeptides with or without the desired biological activity.
• salts means both salts of carboxyl groups and acid addition salts of amino groups of the protein molecules of the invention.
• Salts of carboxyl groups can be prepared in a manner known per se and comprise inorganic salts such as sodium, calcium, ammonium, iron and zinc salts as well as salts with organic bases, for example amines, such as triethanolamine, arginine, lysine, piperidine and the like.
• the invention likewise relates to acid addition salts, for example salts with mineral acids such as hydrochloric acid or sulfuric acid and salts with organic acids such as acetic acid and oxalic acid.
• “Functional derivatives” of polypeptides of the invention may likewise be prepared with the aid of known techniques at functional amino acid side groups or at their N-terminal or C-terminal ends.
• Derivatives of this kind comprise, for example, aliphatic esters of carboxylic acid groups, amides of carboxylic acid groups, which amides are obtainable by reacting with ammonia or with a primary or secondary amine; N-acyl derivatives of free amino groups, which derivatives are prepared by reacting with acyl groups; or O-acyl derivatives of free hydroxyl groups, which derivatives are prepared by reacting with acyl groups.
• “Functional equivalents” naturally also comprise polypeptides which are available from other organisms and also naturally occurring variants. For example, areas of homologous sequence regions can be established by sequence comparison and equivalent enzymes can be determined on the basis of the specific guidelines of the invention.
• “Functional equivalents” likewise comprise fragments, preferably individual domains or sequence motifs, of the polypeptides of the invention, which fragments have, for example, the desired biological function.
• “Functional equivalents” are moreover fusion proteins which contain any of the abovementioned polypeptide sequences or functional equivalents derived therefrom and at least one further heterologous sequence which is functionally different therefrom and is functionally linked N-terminally or C-terminally (i.e. without any substantial reciprocal functional impairment of the fusion protein moieties).
• heterologous sequences are signal peptides or enzymes, for example.
• “Functional equivalents” which are also comprised by the invention are homologs of the specifically disclosed proteins. Said homologs possess at least 60%, preferably at least 75%, in particular at least 85%, such as 90%, 95% or 99%, homology with one of the specifically disclosed amino acid sequences, as calculated using the algorithm of Pearson and Lipman, Proc. Natl. Acad, Sci. (USA) 85(8), 1988, 2444-2448. A percentage homology of a homologous polypeptide of the invention is in particular the percentage identity of the amino acid residues, based on the total length of any of the amino acid sequences specifically described herein.
• “functional equivalents” of the invention comprise proteins of the above-described type in deglycosylated or glycosylated form and also modified forms which can be obtained by altering the glycosylation pattern.
• Homologs of the proteins or polypeptides of the invention may be generated by mutagenesis, for example by point mutation or truncation of the protein.
• Homologs of the proteins of the invention may be identified by screening combinatorial libraries of mutants such as truncation mutants, for example.
• a variegated library of protein variants may be generated by combinatorial mutagenesis at the nucleic acid level, for example by enzymically ligating a mixture of synthetic oligonucleotides.
• a degenerate gene sequence may be synthesized chemically in a DNA synthesizer and the synthetic gene may then be ligated into a suitable expression vector.
• a plurality of techniques for screening gene products of combinatorial libraries which have been prepared by point mutations or truncation and for screening cDNA libraries for gene products having a selected property are known in the prior art. These techniques can be adapted for rapidly screening the gene libraries which have been generated by combinatorial mutagenesis of homologs of the invention.
• the most frequently employed techniques for screening large gene libraries which are subject to high-throughput analysis comprise cloning the gene library into replicable expression vectors, transforming the appropriate cells with the resulting vector library and expressing the combinatorial genes under conditions under which detection of the desired activity facilitates isolation of the vector which encodes the gene whose product was detected.
• REM Recursive ensemble mutagenesis
• the invention relates in particular to nucleic acid sequences (single- and double-stranded DNA and RNA sequences, such as cDNA and mRNA, for example) which code for an enzyme with dehydrogenase activity of the invention. Preference is given to nucleic acid sequences which code, for example, for amino acid sequences according to SEQ ID NO:2 or for characteristic partial sequences thereof, or which comprise nucleic acid sequences according to SEQ ID NO:1 or characteristic partial sequences thereof.
• nucleic acid sequences mentioned herein can be prepared in a manner known per se by chemical synthesis from the nucleotide building blocks, for example by means of fragment condensation of individual overlapping, complementary nucleic acid building blocks of the double helix.
• Oligonucleotides may, for example, be synthesized chemically, in a known manner, using the phosphoamidite method (Voet, Voet, 2nd edition, Wiley Press New York, pages 896-897). The assembly of synthetic oligonucleotides and filling-in of gaps with the aid of the DNA polymerase Klenow fragment and ligation reactions and also general cloning methods are described in Sambrook et al. (1989), Molecular Cloning: A laboratory manual, Cold Spring Harbor Laboratory Press.
• the invention also relates to nucleic acid sequences (single- and double-stranded DNA and RNA sequences, such as cDNA and mRNA, for example) which code for any of the above polypeptides and their functional equivalents which are accessible by using artificial nucleotide analogs, for example.
• nucleic acid sequences single- and double-stranded DNA and RNA sequences, such as cDNA and mRNA, for example
• the invention relates both to isolated nucleic acid molecules which code for polypeptides or proteins of the invention or for biologically active sections thereof and to nucleic acid fragments which may be used, for example, as hybridization probes or primers for identifying or amplifying coding nucleic acids of the invention.
• the nucleic acid molecules of the invention may moreover comprise untranslated sequences from the 3′ and/or the 5′ end of the coding gene region.
• the invention furthermore comprises the nucleic acid molecules complementary to the specifically described nucleotide sequences, or a section thereof.
• probes and primers of the invention make it possible to generate probes and primers which can be used for identifying and/or cloning homologous sequences in other cell types and organisms.
• Probes and primers of this kind usually comprise a nucleotide sequence region which hybridizes, under “stringent” conditions (see below), to at least about 12, preferably at least about 25, such as, for example, about 40, 50 or 75, consecutive nucleotides of a sense strand of a nucleic acid sequence of the invention or of a corresponding antisense strand.
• nucleic acid molecule is removed from other nucleic acid molecules which are present in the natural source of the nucleic acid and may moreover be essentially free of other cellular material or culture medium when it is prepared by means of recombinant techniques or free of chemical precursors or other chemicals when it is synthesized chemically.
• a nucleic acid molecule of the invention may be isolated by means of standard molecular-biological techniques and the sequence information which is provided according to the invention.
• cDNA may be isolated from a suitable cDNA library by using one of the specifically disclosed complete sequences or a section thereof as hybridization probe and using standard hybridization techniques (as described, for example, in Sambrook, J., Fritsch, E. F. and Maniatis, T. Molecular Cloning: A Laboratory Manual. 2nd edition, Cold Spring Harbor Laboratory, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1989).
• nucleic acid molecule comprising any of the disclosed sequences or a section thereof can be isolated using polymerase chain reaction and the oligonucleotide primers which have been constructed on the basis of this sequence.
• the nucleic acid amplified in this way may be cloned into a suitable vector and characterized by DNA sequence analysis.
• the oligonucleotides of the invention may also be prepared by standard synthesis using, for example, an automatic DNA synthesizer.
• nucleic acid sequences of the invention can be identified and isolated in principle from any organisms.
• nucleic acid sequences of the invention or the homologs thereof can be isolated from fungi, yeasts, archeae or bacteria.
• Bacteria which may be mentioned are Gram-negative and Gram-positive bacteria.
• the nucleic acids of the invention are preferably isolated from Gram-negative bacteria, advantageously from ⁇ -proteobacteria, ⁇ -proteobacteria or ⁇ -proteobacteria, particularly preferably from bacteria of the orders Burkholderiales, Hydrogenophilales, Methylophilales, Neisseriales, Nitrosomonadales, Procabacteriales or Rhodocyclales, very particularly preferably from bacteria of the family Rhodocyclaceae, especially preferably from the genus Azoarcus , especially preferably from the species Azoarcus anaerobius, Azoarcus buckelii, Azoarcus communis, Azoarcus evansii, Azoarcus indigens, Azoarcus toluclasticus, Azoarcus tolulyticus, Azoarcus toluvorans, Azoarcus sp., Azoarcus sp.
• Azoarcus sp. BH72 Azoarcus sp. CC-11, Azoarcus sp. CIB, Azoarcus sp. CR23, Azoarcus sp. EB1, Azoarcus sp. EbN1, Azoarcus sp. FL05, Azoarcus sp. HA, Azoarcus sp. H ⁇ N1, Azoarcus sp. mXyN1, Azoarcus sp. PbN1, Azoarcus sp. PH002, Azoarcus sp. T and Azoarcus sp. ToN1.
• Nucleic acid sequences of the invention can, for example, be isolated from other organisms by using customary hybridization methods or the PCR technique, for example by way of genomic or cDNA libraries. These DNA sequences hybridize with the sequences of the invention under standard conditions. Use is advantageously made, for the hybridization, of short oligonucleotides of the conserved regions, for example from the active site, which conserved regions may be identified in a manner known to the skilled worker by way of comparisons with a dehydrogenase of the invention. However, it is also possible to use longer fragments of the nucleic acids of the invention or the complete sequences for the hybridization.
• Said standard conditions vary depending on the nucleic acid employed (oligonucleotide, longer fragment or complete sequence) or depending on which nucleic acid type, DNA or RNA, is used for the hybridization.
• the melting temperatures for DNA:DNA hybrids are approx. 10° C. lower than those for DNA:RNA hybrids of the same length.
• the hybridization conditions for DNA:DNA hybrids are 0.1 ⁇ SSC and temperatures between about 20° C. to 45° C., preferably between about 30° C. to 45° C.
• the hybridization conditions are advantageously 0.1 ⁇ SSC and temperatures between about 30° C. to 55° C., preferably between about 45° C. to 55° C.
• the temperatures indicated for the hybridization are melting temperature values which have been calculated by way of example for a nucleic acid having a length of approx. 100 nucleotides and a G+C content of 50% in the absence of formamide.
• the experimental conditions for the DNA hybridization are described in specialist textbooks of genetics, such as, for example, Sambrook et al., “Molecular Cloning”, Cold Spring Harbor Laboratory, 1989, and can be calculated using formulae known to the skilled worker, for example as a function of the length of the nucleic acids, the type of hybrids or the G+C content. The skilled worker can obtain further information with regard to hybridization from the following textbooks: Ausubel et al.
• the invention also relates to derivatives of the specifically disclosed or derivable nucleic acid sequences.
• nucleic acid sequences of the invention may be derived from SEQ ID NO:1 and differ therefrom by the addition, substitution, insertion or deletion of single or two or more nucleotides but still code for polypeptides having the desired property profile.
• the invention also comprises those nucleic acid sequences which comprise “silent” mutations or have been altered, as compared with a specifically mentioned sequence, according to the codon usage of a specific source organism or host organism, as well as naturally occurring variants thereof, such as splice variants or allele variants, for example.
• the invention also relates to sequences obtainable by way of conservative nucleotide substitutions (i.e. the amino acid in question is replaced with an amino acid of the same charge, size, polarity and/or solubility).
• the invention also relates to the molecules which are derived from the specifically disclosed nucleic acids by way of sequence polymorphisms. These genetic polymorphisms can exist between individuals within a population as a result of natural variation. These natural variations usually give rise to a variance of from 1 to 5% in the nucleotide sequence of a gene.
• Derivatives of a nucleic acid sequence of the invention mean, for example, allele variants which, at the deduced amino acid level, are at least 40%, preferably at least 60%, very particularly preferably at least 80, 85, 90, 93, 95 or 98%, homologous over the entire sequence region (with respect to homology at the amino acid level, the reader may refer to the above comments regarding the polypeptides).
• the homologies may be higher across subregions of the sequences.
• derivatives also mean homologs of the nucleic acid sequences of the invention, for example fungal or bacterial homologs, truncated sequences, single-stranded DNA or RNA of the coding and noncoding DNA sequence.
• homologs of the nucleic acid sequences of the invention for example fungal or bacterial homologs, truncated sequences, single-stranded DNA or RNA of the coding and noncoding DNA sequence.
• homologs of the nucleic acid sequences of the invention for example fungal or bacterial homologs, truncated sequences, single-stranded DNA or RNA of the coding and noncoding DNA sequence.
• homologs of the nucleic acid sequences of the invention for example fungal or bacterial homologs, truncated sequences, single-stranded DNA or RNA of the coding and noncoding DNA sequence.
• derivatives mean fusions with promoters, for example.
• the promoters which are located upstream of the nucleotide sequences indicated may have been altered by one or more nucleotide replacements, insertions, inversions and/or deletions without, however, the functionality and efficacy of the promoters being impaired.
• the efficacy of said promoters may be increased by altering their sequence or the promoters may be completely replaced with more active promoters, including those from organisms of other species.
• Derivatives also mean variants whose nucleotide sequence in the region from ⁇ 1 to ⁇ 1000 bases upstream of the start codon or from 0 to 1000 bases downstream of the stop codon has been altered so as to alter, preferably increase, gene expression and/or protein expression.
• the invention furthermore comprises nucleic acid sequences which hybridize with coding sequences mentioned above under “stringent conditions”.
• These polynucleotides can be found by screening genomic or cDNA libraries and, if appropriate, amplified therefrom by means of PCR using suitable primers and then isolated using suitable probes, for example.
• polynucleotides of the invention may also be synthesized chemically. This property means the ability of a polynucleotide or oligonucleotide to bind to a virtually complementary sequence under stringent conditions while, under these conditions, unspecific bonds between noncomplementary partners are not formed.
• the sequences should be 70-100%, preferably 90-100%, complementary.
• stringent conditions mean the use of a washing solution of 50-70° C., preferably 60-65° C., for example 0.1 ⁇ SSC buffer containing 0.1% SDS (20 ⁇ SSC: 3M NaCl, 0.3M sodium citrate, pH 7.0), for eluting unspecifically hybridized cDNA probes or oligonucleotides.
• stringent conditions mean the use of a washing solution of 50-70° C., preferably 60-65° C., for example 0.1 ⁇ SSC buffer containing 0.1% SDS (20 ⁇ SSC: 3M NaCl, 0.3M sodium citrate, pH 7.0), for eluting unspecifically hybridized cDNA probes or oligonucleotides.
• SDS 3M NaCl
• 0.3M sodium citrate pH 7.0
• the invention moreover relates to expression constructs comprising, under the genetic control of regulatory nucleic acid sequences, a nucleic acid sequence coding for a polypeptide of the invention; and also to vectors comprising at least one of these expression constructs.
• Such constructs of the invention preferably comprise a promoter 5′-upstream of the particular coding sequence and a terminator sequence 3′-downstream and also, if appropriate, further customary regulatory elements which are in each case operatively linked to the coding sequence.
• An “operative linkage” means the sequential arrangement of promoter, coding sequence, terminator and, if appropriate, further regulatory elements in such a way that each of the regulatory elements is able to fulfil its function as required in expressing the coding sequence.
• operatively linkable sequences are targeting sequences and also enhancers, polyadenylation signals and the like.
• Other regulatory elements comprise selectable markers, amplification signals, origins of replication and the like. Suitable regulatory sequences are described, for example, in Goeddel, Gene Expression Technology Methods in Enzymology 185, Academic Press, San Diego, Calif. (1990).
• a nucleic acid construct of the invention means in particular those in which the gene for a dehydrogenase of the invention has been operatively or functionally linked to one or more regulatory signals for the purpose of regulating, e.g. increasing, expression of the gene.
• the natural regulation of these sequences may still be present upstream of the actual structural genes and, if appropriate, may have been genetically altered in such a way that the natural regulation has been switched off and expression of the genes has been increased.
• the nucleic acid construct may also have a simpler design, i.e. no additional regulatory signals have been inserted upstream of the coding sequence and the natural promoter, together with its regulation, has not been removed. Instead of this, the natural regulatory sequence is mutated in such a way that there is no longer any regulation and expression of the gene is increased.
• a preferred nucleic acid construct also advantageously comprises one or more of the previously mentioned enhancer sequences which are functionally linked to the promoter and which enable expression of the nucleic acid sequence to be increased. Additional advantageous sequences such as further regulatory elements or terminators may also be inserted at the 3′ end of the DNA sequences.
• the nucleic acids of the invention may be present in the construct in one or more copies.
• the construct may also comprise additional markers such as antibiotic resistances or auxotrophy-complementing genes, if appropriate for the purpose of selecting said construct.
• Regulatory sequences which are advantageous for the process of the invention are present, for example, in promoters such as the cos, tac, trp, tet, trp-tet, lpp, lac, lpp-lac, lacI q , T7, T5, T3, gal, trc, ara, rhaP (rhaPBAD) SP6, lambda-P R or lambda-P L promoter, which promoters are advantageously used in Gram-negative bacteria.
• promoters such as the cos, tac, trp, tet, trp-tet, lpp, lac, lpp-lac, lacI q , T7, T5, T3, gal, trc, ara, rhaP (rhaPBAD) SP6, lambda-P R or lambda-P L promoter, which promoters are advantageously used in Gram-negative bacteria.
• promoters amy and SPO2 in the yeast or fungal promoters ADC1, MFalpha, AC, P-60, CYC1, GAPDH, TEF, rp28, ADH.
• the pyruvate decarboxylase and methanoloxidase promoters are also advantageous in this connection. It is also possible to use artificial promoters for regulation.
• the nucleic acid construct is advantageously inserted into a vector such as a plasmid or a phage, for example, which enables the genes to be expressed optimally in the host.
• Vectors mean, in addition to plasmids and phages, also any other vectors known to the skilled worker, i.e., for example, viruses such as SV40, CMV, baculovirus and adenovirus, transposons, IS elements, phasmids, cosmids, and linear or circular DNA. These vectors may be replicated autonomously in the host organism or replicated chromosomally. These vectors constitute a further embodiment of the invention.
• plasmids examples include pLG338, pACYC184, pBR322, pUC18, pUC19, pKC30, pRep4, pHS1, pKK223-3, pDHE19.2, pHS2, pPLc236, pMBL24, pLG200, pUR290, pIN-III 113 -B1, Igt11 or pBdCl, in E.
• coli coli , plJ101, plJ364, plJ702 or plJ361, in Streptomyces , pUB110, pC194 or pBD214, in Bacillus , pSA77 or pAJ667, in Corynebacterium , pALS1, plL2 or pBB116, in fungi, 2alphaM, pAG-1, YEp6, YEp13 or pEMBLYe23, in yeasts, or pLGV23, pGHlac + , pBIN19, pAK2004 or pDH51, in plants.
• Said plasmids are a small selection of the possible plasmids.
• the nucleic acid construct advantageously also comprises 3′-terminal and/or 5′-terminal regulatory sequences for increasing expression, which are selected for optimal expression in dependence on the host organism and the gene or genes selected.
• regulatory sequences are intended to enable the genes and protein expression to be specifically expressed. Depending on the host organism, this may mean, for example, that the gene is expressed or overexpressed only after induction or that it is expressed and/or overexpressed immediately.
• the regulatory sequences or factors may preferably influence positively and thereby increase expression of the genes which have been introduced.
• the regulatory elements may advantageously be enhanced at the level of transcription by using strong transcription signals such as promoters and/or enhancers.
• strong transcription signals such as promoters and/or enhancers.
• the vector which comprises the nucleic acid construct of the invention or the nucleic acid of the invention may also advantageously be introduced into the microorganisms in the form of a linear DNA and be integrated into the genome of the host organism by way of heterologous or homologous recombination.
• This linear DNA may consist of a linearized vector such as a plasmid or only of the nucleic acid construct or the nucleic acid of the invention.
• An expression cassette of the invention is prepared by fusing a suitable promoter to a suitable coding nucleotide sequence and to a terminator signal or polyadenylation signal.
• Common recombination and cloning techniques as are described, for example, in T. Maniatis, E. F. Fritsch and J. Sambrook, Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y. (1989) and also in T. J. Silhavy, M. L. Berman and L. W. Enquist, Experiments with Gene Fusions, Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y. (1984) and in Ausubel, F. M. et al., Current Protocols in Molecular Biology, Greene Publishing Assoc. and Wiley Interscience (1987) are used for this purpose.
• the recombinant nucleic acid construct or gene construct is advantgeously inserted into a host-specific vector which enables the genes to be expressed optimally in the host.
• Vectors are well known to the skilled worker and may be found, for example, in “Cloning Vectors” (Pouwels P. H. et al., Eds., Elsevier, Amsterdam-New York-Oxford, 1985).
• recombinant microorganisms which are, for example, transformed with at least one vector of the invention and which may be used for producing the polypeptides of the invention.
• the above-described recombinant constructs of the invention are introduced into a suitable host system and expressed.
• familiar cloning and transfection methods known to the skilled worker such as, for example, coprecipitation, protoplast fusion, electroporation, retroviral transfection and the like, are preferably used in order to cause said nucleic acids to be expressed in the particular expression system. Suitable systems are described, for example, in Current Protocols in Molecular Biology, F.
• a vector which comprises at least one section of a gene of the invention or of a coding sequence in which, if appropriate, at least one amino acid deletion, amino acid addition or amino acid substitution has been introduced in order to modify, for example functionally disrupt, the sequence of the invention is prepared.
• the introduced sequence may also be a homolog from a related microorganism or be derived from a mammalian, yeast or insect source, for example.
• the vector used for homologous recombination may be designed in such a way that the endogenous gene is, in the case of homologous recombination, mutated or otherwise altered but still encodes the functional protein (e.g. the upstream regulatory region may have been altered in such a way that expression of the endogenous protein is thereby altered).
• the altered section of the gene of the invention is in the homologous recombination vector.
• Recombinant host organisms suitable for the nucleic acid of the invention or the nucleic acid construct are in principle any prokaryotic or eukaryotic organisms.
• microorganisms such as bacteria, fungi or yeasts are used as host organisms.
• Gram-positive or gram-negative bacteria preferably bacteria of the families Enterobacteriaceae, Pseudomonadaceae, Rhizobiaceae, Streptomycetaceae or Nocardiaceae, particularly preferably bacteria of the genera Escherichia, Pseudomonas, Streptomyces, Nocardia, Burkholderia, Salmonella, Agrobacterium or Rhodococcus , are advantageously used. Very particular preference is given to the genus and species Escherichia coli .
• further advantageous bacteria can be found in the group of the alpha-proteobacteria, beta-proteobacteria or gamma-proteobacteria.
• the host organism or host organisms of the invention comprise(s) preferably at least one of the nucleic acid sequences, nucleic acid constructs or vectors which are described in this invention and which encode an enzyme with dehydrogenase activity of the invention.
• the organisms used in the process of the invention are, depending on the host organism, grown or cultured in a manner known to the skilled worker.
• Microorganisms are usually grown in a liquid medium which comprises a carbon source, usually in the form of sugars, a nitrogen source, usually in the form of organic nitrogen sources such as yeast extract or salts such as ammonium sulfate, trace elements such as iron salts, manganese salts, magnesium salts and, if appropriate, vitamins, at temperatures of between 0° C. and 100° C., preferably between 10° C. and 60° C., while being gassed with oxygen.
• the pH of the nutrient liquid may or may not be kept at a fixed value, i.e. may or may not be regulated during cultivation.
• the cultivation may be carried out batchwise, semibatchwise or continuously. Nutrients may be introduced at the beginning of the fermentation or be fed in subsequently in a semicontinuous or continuous manner.
• the ketone may be added directly to the culture or, advantageously, after cultivation.
• the enzymes may be isolated from the organisms by using the method described in the examples or be used for the reaction as a crude extract.
• the invention furthermore relates to processes for recombinantly preparing polypeptides of the invention or functional, biologically active fragments thereof, with a polypeptide-producing microorganism being cultured, if appropriate expression of the polypeptides being induced and said polypeptides being isolated from the culture.
• the polypeptides may also be produced in this way on an industrial scale if this is desired.
• the recombinant microorganism may be cultured and fermented by known methods.
• Bacteria may, for example, be propagated in TB medium or LB medium and at a temperature of from 20 to 40° C. and a pH of from 6 to 9. Suitable culturing conditions are described in detail, for example, in T. Maniatis, E. F. Fritsch and J. Sambrook, Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y. (1989).
• the cells are then disrupted and the product is obtained from the lysate by known protein isolation methods.
• the cells may be disrupted, as desired, by means of high-frequency ultrasound, by means of high pressure, as, for example, in a French pressure cell, by means of osmolysis, by the action of detergents, lytic enzymes or organic solvents, by using homogenizers or by a combination of two or more of the methods listed.
• the polypeptides may be purified using known chromatographic methods such as molecular sieve chromatography (gel filtration), for example Q Sepharose chromatography, ion exchange chromatography and hydrophobic chromatography, and also using other customary methods such as ultra filtration, crystallization, salting-out, dialysis and native gel electrophoresis. Suitable methods are described, for example, in Cooper, F. G., Biochemische Harvey [original title: The tools of biochemistry], Verlag Walter de Gruyter, Berlin, New York or in Scopes, R., Protein Purification, Springer Verlag, New York, Heidelberg, Berlin.
• chromatographic methods such as molecular sieve chromatography (gel filtration), for example Q Sepharose chromatography, ion exchange chromatography and hydrophobic chromatography, and also using other customary methods such as ultra filtration, crystallization, salting-out, dialysis and native gel electrophoresis. Suitable methods are described, for example, in Cooper, F. G.,
• telomeres may be advantageous to isolate the recombinant protein by using vector systems or oligonucleotides which extend the cDNA by particular nucleotide sequences and thereby code for altered polypeptides or fusion proteins which are used, for example, to simplify purification.
• suitable modifications of this kind are, for example, “tags” acting as anchors, such as the modification known as the hexa-histidine anchor, or epitopes which can be recognized as antigens by antibodies (described, for example, in Harlow, E. and Lane, D., 1988, Antibodies: A Laboratory Manual. Cold Spring Harbor (N.Y.) Press).
• These anchors may be used for attaching the proteins to a solid support such as a polymer matrix, for example, which may, for example, be packed into a chromatography column, or to a microtiter plate or to another support.
• these anchors may also be used for identifying the proteins.
• the proteins may also be identified by using customary markers such as fluorescent dyes, enzyme markers which, after reaction with a substrate, form a detectable reaction product, or radioactive markers, either on their own or in combination with the anchors, for derivatizing said proteins.
• the enzymes with dehydrogenase activity which are used according to the invention may be used as free or immobilized enzymes in the process of the invention.
• the process of the invention is advantageously carried out at a temperature between 0° C. to 95° C., preferably between 10° C. to 85° C., particularly preferably between 15° C. to 75° C.
• the pH is advantageously kept between pH 4 and 12, preferably between pH 4.5 and 9, particularly preferably between pH 5 and 8.
• enantiomerically pure or chiral products or optically active alcohols mean enantiomers which exhibit enantiomer enrichment.
• Enantiomeric purities of at least 70% ee, preferably of at least 80% ee, particularly preferably of at least 90% ee, very particularly preferably of at least 98% ee, are preferably achieved in the process.
• Disrupted cells mean, for example, cells which have been made permeable by way of treatment with solvents, for example, or cells which have been broken up by way of treatment with enzymes, by way of mechanical treatment (e.g. French press or ultrasonication) or by way of another method.
• the crude extracts obtained in this manner are advantageously suitable for the process of the invention.
• purified or partially purified enzymes for the process. Immobilized microorganisms or enzymes which may advantageously be applied in the reaction are likewise suitable.
• Free organisms or enzymes are, when used for the process of the invention, conveniently removed prior to extraction, for example via filtration or centrifugation.
• the product produced in the process of the invention for example (1S)-3-methylamino-1-(2-thienyl)propan-1-ol can advantageously be obtained from the aqueous reaction solution by way of extraction or distillation.
• the extraction may be repeated several times.
• suitable extractants are solvents such as toluene, methylene chloride, butyl acetate, diisopropyl ether, benzene, MTBE or ethyl acetate, without being limited thereto.
• the product prepared in the process of the invention for example (1S)-3-methylamino-1-(2-thienyl)-propan-1-ol may advantageously be isolated from the organic phase of the reaction solution via extraction or distillation or/and crystallization. The extraction may be repeated several times in order to increase the yield.
• suitable extractants are solvents such as toluene, methylene chloride, butyl acetate, diisopropyl ether, benzene, MTBE or ethyl acetate, without being limited thereto.
• the products can usually be obtained with good chemical purities, i.e. more than 80% chemical purity.
• the organic phase containing the product may, however, also be concentrated only partially and the product may be crystallized.
• the solution is advantageously cooled to a temperature of from 0° C. to 10° C.
• the crystallization may also be carried out directly from the organic solution or from an aqueous solution.
• the crystallized product may be resuspended in the same or in a different solvent for the purpose of renewed crystallization and recrystallized.
• the subsequent advantageous crystallization which is carried out at least once may, if necessary, further increase the enantiomeric purity of the product.
• the work-up types mentioned enable the product of the process of the invention to be isolated with yields of from 60 to 100%, preferably from 80 to 100%, particularly preferably from 90 to 100%, based on the substrate used for the reaction, such as, for example, 3-methylamino-1-(2-thienyl)propan-1-one.
• the isolated product is characterized by a high chemical purity of >90%, preferably >95% particularly preferably of >98%.
• the products furthermore have high enantiomeric purity which can advantageously be increased further by the crystallization, if necessary.
• the process of the invention can be operated batchwise, semi-batchwise or continuously.
• the process may advantageously be carried out in bioreactors as described, for example, in Biotechnology, volume 3, 2nd edition, Rehm et al. Eds., (1993), in particular chapter II.
• the advantage of the process of the invention is the particularly high yield of the optically active alkanol of the formula (I) or the virtually quantitative conversion of alkanone (II).
• the sequence of the gene of Azoarcus sp EbN1 phenylethanol dehydrogenase has been deposited in databases (Genbank ID 25956124, region: 25073 to 25822). Oligonucleotides from which the gene was synthesized according to known methods were derived from the nucleic acid sequence of the phenylethanol dehydrogenase gene. Table 1 summarizes the DNA sequence of said oligonucleotides. FIG. 3 indicates the position of the individual oligonucleotides in relation to the entire Azoarcus sp EbN1 phenylethanol dehydrogenase gene. Methods of preparing synthetic genes have been described, for example, in Y. P. Shi, P. Das, B.
• the PCR product was digested with the restriction endonucleases NdeI and BamHI and cloned into the pDHE19.2 vector (DE19848129) which had been digested accordingly.
• the ligation mixtures were transformed into E. coli XL1 Blue (Stratagene).
• the pDHEEbN1 plasmid was transformed into the E. coli strain TG10 pAgro4 pHSG575 (TG10: an RhaA-derivative of E.
• coli TG1 (Stratagene); pAgro4: Takeshita, S; Sato, M; Toba, M; Masahashi, W; Hashimoto-Gotoh, T (1987) Gene 61, 63-74; pHSG575: T. Tomoyasu et al (2001), Mol. Microbiol. 40(2), 397-413).
• E. coli LU 11558 The recombinant E. coli are referred to as E. coli LU 11558.
• the PCR product ( ⁇ 0.75 kB) was isolated by agarose gel electrophoresis (1.2% E-Gel, Invitrogen) and column chromatography (GFX kit, Amersham Pharmacia) and then sequenced (sequencing primers: MKe0387 and MKe0388). The sequence obtained is identical to the published sequence.
• the PCR product was digested with the restriction endonucleases NdeI and BamHI and cloned into the pDHE19.2 vector (DE19848129) which had been digested accordingly.
• the ligation mixtures were transformed into E. coli XL1 Blue (Stratagene).
• the insert in the plasmid obtained in this way, pDHEEbN1 which insert was revealed by sequencing corresponding clones, is the nucleic acid sequence depicted in SEQ ID NO: 1.
• the pDHEEbN1 plasmid was transformed into the E. coli strain TG10 pAgro4 pHSG575 (TG10: an RhaA ⁇ derivative of E. coli TG1 (Stratagene); pAgro4: Takeshita, S; Sato, M; Toba, M; Masahashi, W; Hashimoto-Gotoh, T (1987) Gene 61, 63-74; pHSG575: T. Tomoyasu et al (2001), Mol. Microbiol. 40(2), 397413).
• the recombinant E. coli are referred to as E. coli LU 11558.
• 100 ml of cell suspension were incubated with shaking for 20 minutes in 900 ⁇ l of 50 mM MES pH 6 containing 50 ⁇ l/ml glucose DH, 100 mM glucose, 100 mM NaCl, 1 mM NADH, 1 mM NADPH and with 10 mM 3-chloro-1-(thien-2-yl)propan-1-one.
• the reaction mixtures were analyzed in a manner analogous to example 4. On average, 0.13 mM 3-chloro-1-(thien-2-yl)propan-1-ol was produced, corresponding to an activity of 6.6 U/l of culture suspension.
• concentrations of 3-chloro-1-(thien-2-yl)propan-1-one and 3-chloro-1-(thien-2-yl)propan-1-ol can be determined by means of HPLC. It is also possible, depending on the choice of stationary and mobile phases, to determine the ee value in addition to the concentration.
• the reaction was quantified using the following system:
• 100 ml of cell suspension were incubated with shaking for 20 minutes in 900 ⁇ l of 50 mM MES pH 6 containing 50 ⁇ l/ml glucose DH (example . . . ), 100 mM glucose, 100 mM NaCl, 1 mM NADH, 1 mM NADPH and with 10 mM 3-chloro-1-(thien-2-yl)propan-1-one.
• the reaction mixtures were analyzed in a manner analogous to example 4. On average, 0.13 mM 3-chloro-1-(thien-2-yl)propan-1-ol was produced, corresponding to an activity of 6.6 U/l of culture suspension.
• the distilled amounts of water and 2-pentanol obtained at rates of distillation of 2-10 ml/min were reintroduced continuously during the reaction.
• the reaction was monitored by HPLC analysis up to a concentration of 300-700 mM TACA in the organic phase.
• the 3-chloro-1-(thien-2-yl)propan-1-one was reacted down to a residual portion of 0-30%.
• SEQ ID NO:1 Nucleic acid sequence of Azoarcus sp EbN1 phenylethanol dehydrogenase (Genbank ID 25956124, region:25073 to 25822)
• SEQ ID NO:2 Amino acid sequence of Azoarcus sp EbN1 phenylethanol dehydrogenase accession (Genbank protein ID CAD58337)

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