Classifications

• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D417/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00
  • C07D417/14—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing three or more hetero rings
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D277/00—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings
  • C07D277/02—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings not condensed with other rings
  • C07D277/04—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings not condensed with other rings having no double bonds between ring members or between ring members and non-ring members
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P3/00—Drugs for disorders of the metabolism
  • A61P3/04—Anorexiants; Antiobesity agents
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P3/00—Drugs for disorders of the metabolism
  • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
  • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00

Definitions

• the present invention relates to a thiazolidine derivative showing a dipeptidyl peptidase IV (DPP-IV) inhibitory activity and useful for the prophylaxis or treatment of diseases in which DPP-IV is involved, particularly, diabetes, obesity and the like, and a salt thereof.
• DPP-IV dipeptidyl peptidase IV
• DPP-IV is a serine protease that recognizes an amino acid sequence having proline (may be alanine, hydroxyproline) at the penultimate position from the N-terminal and produces dipeptide Xaa-Pro (wherein Xaa shows an optional amino acid and Pro shows L-proline). It is known that DPP-IV is widely distributed in the mammalian tissues, particularly in blood, kidney, intestinal epitherlium and placenta.
• DPP-IV DPP-IV in mammal
• its involvement in a broad range of biological functions such as degradation of neuro-peptide [Heymann et al., FEBS Letters, vol. 91, pp. 360-364 (1978)], activation of T cell [Schon et al., Biomedica Biochimica Acta, vol. 44, pp. K9-K15 (1985)], adhesion of metastatic tumor cells to endothelium [Johnson et al., Journal of Cell Biology, vol. 121, pp. 1423-1432 (1993)], invasion of HIV virus into lymphocyte [Callebaut et al., Science, vol. 262, pp.
• DPP-IV as an enzyme that inactivates a biological substance glucagon-like peptide (GLP-1) having a potent insulin secretagogue action, which controls postprandial blood glucose level, has been drawing attention [Deacon et al., Journal of Clinical Endocrinology and Metabolism, vol. 80, pp. 952-957 (1995)].
• GLP-1 glucagon-like peptide
• GLP-1 is metabolized in several minutes in living organisms.
• metabolism by DPP-V is particularly important, where it rapidly cleaves GLP-1 and produces inactive GLP-1 [Deacon et al., American Journal of Physiology, vol. 271, pp. E458-E464 (1996)].
• the inactive GLP-1 shows an antagonistic action against GLP-1 receptor, the physiological action of GLP-1 is considered to be further attenuated [Knudsen et al., European Journal of Pharmacology, vol. 318, pp. 429-435 (1996)]. Therefore, a method for suppressing degradation of GLP-1 by DPP-IV inhibition is considered the best approach for enhancing the GLP-1 action.
• a DPP-IV inhibitor is expected to be a superior treatment method for correcting postprandial hyperglycemia of non-insulin dependent diabetic (type 2 diabetes) patients, without causing side effects such as prolonged hypoglycemia and the like.
• Patent applications relating to DPP-IV inhibitors include the following.
• JP-T-9-509921 discloses 1-[N- ⁇ -(hydroxysuccinyl)-L-lysyl]pyrrolidine. This L-lysine moiety is limited to an acyl-substituted form.
• JP-T-9-509921 discloses (S)-2-cyano-1-L-prolinepyrrolidine derivative.
• the L- ⁇ -amino acid corresponding to the L-proline moiety of the compound disclosed therein is characterized in that it has a hydrophobic side chain.
• WO99/61431 describes that a compound comprising natural amino acid and thiazolidine or pyrrolidine, shows a DPP-IV inhibitory activity.
• the present inventors conducted intensive studies with the aim of developing a novel DPP-IV inhibitor. As a result, they found that a thiazolidine derivative having a hydrophilic amino group introduced into the side chain and a derivative having a substituent introduced into the ⁇ -position of proline have a potent DPP-IV inhibitory activity, and they further enhanced the stability, which resulted in the completion of the present invention.
• the present invention relates to the following compounds.
• m is an integer of 1 or 2
• n is an integer of 1 to 5
• X′ is a hydrogen atom or an alkyl optionally having substituent(s),
• Y′ is represented by —NR 1 R 2 (R 1 is aryl optionally having substituent(s) or heteroaryl optionally having substituent(s), R 2 is a hydrogen atom, alkyl optionally having substituent(s), aryl optionally having substituent(s), arylalkyl optionally having substituent(s), heteroaryl optionally having substituent(s) or heteroarylalkyl optionally having substituent(s), or they may be bonded to each other to form a heterocycle having carbon and at least one nitrogen, optionally having other heteroatom(s) and optionally having substituent(s), where this heterocycle is optionally substituted or condensed with an aromatic ring optionally having substituent(s)),
• [0019] is a single bond or a double bond
• A is a carbon atom or a nitrogen atom
• Q is aryl or heteroaryl selected from the compounds represented by the following formulas (II)-(XII):
• R 1a is alkyl, phenyl, pyridyl, pyrimidinyl, imidazolyl or oxazolyl, wherein these groups are optionally substituted by one or more from alkyl, alkoxy, halogen and cyano,
• R 2a is a hydrogen atom, alkyl or haloalkyl
• one of e and f is a nitrogen atom and the other is a carbon atom, or both are carbon atoms (same as “0-1 of e and f is a nitrogen atom and the rest is(are) carbon atom(s)”), and
• R 3 and R 4 may be the same or different and each is a hydrogen atom, alkyl, phenyl or pyridyl,
• j is a sulfur atom, an oxygen atom or a nitrogen atom
• h and i may be the same or different and each is a nitrogen atom or a carbon atom (same as “0-2 of h and i is(are) nitrogen atom(s) and the rest is(are) carbon atom(s)”),
• R 5 and R 7 may be the same or different and each is a hydrogen atom, phenyl or pyridyl (when h is a nitrogen atom, then R 7 is absent), and
• R 6 is a hydrogen atom or alkyl (when i is a nitrogen atom, then R 6 is absent),
• k, l and n′ may be the same or different and each is a carbon atom or a nitrogen atom, wherein at least one is a carbon atom (same as “0-2 of k, l and n′ is(are) nitrogen atom(s) and the rest is(are) carbon atom(s)”),
• R 8 is a hydrogen atom, phenyl, pyridyl or nitro (when n′ is a nitrogen atom, then R 8 is absent),
• R 8a is a hydrogen atom or phenyl
• R 9 is a hydrogen atom, haloalkyl or cyano
• R 10 is a hydrogen atom or cyano (when 1 is a nitrogen atom, then R 10 is absent),
• R 11 is a hydrogen atom, phenyl or pyridyl (when p is a nitrogen atom, then R 11 is phenyl or pyridyl),
• R 12 is a hydrogen atom or alkyl (when p is a nitrogen atom, then R 12 is absent), and
• R 13 and R 14 are both hydrogen atoms, or when one of them is a hydrogen atom, then the other is cyano, alkoxy or halogen,
• R 15 is a hydrogen atom, alkyl or phenyl (when r is a nitrogen atom, then R 15 is absent),
• R 16 is a hydrogen atom or alkyl (when s is a nitrogen atom, then R 16 is absent), and
• R 17 is a hydrogen atom, haloalkyl or cyano
• r′ and s′ may be the same or different and each is a carbon atom or a nitrogen atom, wherein at least one of them is a nitrogen atom (same as “1-2 of r′ and s′ is(are) nitrogen atom(s) and the rest is(are) carbon atom(s)”),
• R 15a is a hydrogen atom, alkyl or phenyl (when r′ is a nitrogen atom, then R 15a is absent),
• R 16a is a hydrogen atom or alkyl (when r′ and s′ are both nitrogen atoms, then R 16a is a hydrogen atom), and
• R 17a is a hydrogen atom, haloalkyl or cyano
• t is a sulfur atom or an oxygen atom
• u is a carbon atom or a nitrogen atom
• R 18 and R 19 are both hydrogen atoms, or one of them is a hydrogen atom and the other is cyano, alkoxy or halogen,
• a in the formula (I-b) is a nitrogen atom, t is an oxygen atom, R 19 is a hydrogen atom, and when u is a carbon atom, then R 18 is alkoxy or halogen, and
• a in the formula (I-b) is a carbon atom, R 19 is a hydrogen atom, u is a carbon atom, and when t is an oxygen atom, R 18 is halogen,
• v, w, x and y may be the same or different and each is a carbon atom or a nitrogen atom, wherein at least two are carbon atoms (same as “0-2 of v, w, x and y is(are) nitrogen atom(s) and the rest is(are) carbon atom(s)”),
• R 20 , R 21 , R 22 , R 23 , R 24 , R 25 and R 26 may be the same or different and 1 to 3 is(are) haloalkyl, methoxy, ethoxy, isopropoxy, trifluoromethoxy, 2,2,2-trifluoroethoxy, hydroxy, cyano or halogen, and the rest is(are) hydrogen atom(s) (when v is a nitrogen atom, then R 20 is absent, when w is a nitrogen atom, then R 22 is absent, when x is a nitrogen atom, then R 23 is absent, when y is a nitrogen atom, then R 25 is absent),
• R 21 is a hydrogen atom and R 20 is cyano
• R 21 is haloalkyl and R 23 is hydroxy, ethoxy, isopropoxy, trifluoromethoxy or 2,2,2-trifluoroethoxy
• R 21 is haloalkyl and R 23 and R 25 are both methoxy
• R 21 is haloalkyl and R 24 is hydroxy, chloro or trifluoromethyl
• R 21 is haloalkyl and R 25 is hydroxy or trifluoromethoxy
• R 21 is haloalkyl and R 26 is methoxy
• R 21 is cyano and R 23 is methoxy
• R 27 and R 28 may be the same or different and each is haloalkyl or alkoxy;
• Y is methylene, hydroxymethylene, a sulfur atom, sulfinyl or sulfonyl;
• Z is a hydrogen atom or cyano
• [0084] is a single bond or a double bond
• R 2 is as defined in claim 1 ,
• R 3a and R 4a are the same or different and each is independently hydrogen atom, alkyl optionally having substituent(s), aryl optionally having substituent(s), arylalkyl optionally having substituent(s), heteroaryl optionally having substituent(s), heteroarylalkyl optionally having substituent(s), halogen, haloalkyl, cyano, nitro, —NR 5a R 6a , —NHSO 2 R 7a , —OR 8b , —COOR 9a , —CONHSO 2 R 10a , —SO 2 OR 11a , —SO 2 R 12a or —CONR 13a R 14a wherein R 5a , R 6a , R 7a , R 8b , R 9a ,
• R 10a , R 11a , R 12a , R 13a and R 14a are the same or different and each is independently a hydrogen atom, alkyl optionally having substituent(s), cycloalkyl optionally having substituent(s), cycloalkylalkyl optionally having substituent(s), aryl optionally having substituent(s), arylalkyl optionally having substituent(s), heteroaryl optionally having substituent(s), heteroarylalkyl optionally having substituent(s) or haloalkyl, R 5a and R 6a ,
• R 13a and R 14a may be bonded to each other to form a heterocycle having carbon and at least one nitrogen, optionally having other heteroatom(s) and optionally having substituent(s), where this heterocycle is optionally substituted or condensed with an aromatic ring optionally having substituent(s),
• a′, b′, c′, d′, e′, f′ and g′ are all carbon atoms, or any one or two thereof is(are) nitrogen atom(s), and the rest is(are) carbon atom(s),
• m′ is 0, 1, 2 or 3
• A′ is a carbon atom or a nitrogen atom
• A′ when A′ is a carbon atom, then A′ may be substituted by a hydroxyl group, carboxy or alkoxycarbonyl, and ii) when A′ is a nitrogen atom, then
• a pharmaceutical composition comprising the thiazolidine derivative of any of the above-mentioned [1]-[8] or a pharmaceutically acceptable salt thereof, and a pharmacologically acceptable carrier.
• a DPP-IV inhibitor comprising the thiazolidine derivative of any of the above-mentioned [1]-[8] or a pharmaceutically acceptable salt thereof.
• a therapeutic agent of a disease, in which DPP-IV is involved which comprises the thiazolidine derivative of any of the above-mentioned [1]-[8] or a pharmaceutically acceptable salt thereof as an active ingredient.
• the alkyl represented by X′, R 1a , R 2 , R 2a , R 3 , R 3a , R 4 , R 4a , R 5a , R 6 , R 6a , R 7a , R 8b , R 9a , R 10a , R 11a , R 12 , R 12a , R 13a , R 14a , R 15 , R 16 , R 15a or R 16a preferably means a linear or branched chain alkyl having 1 to 8 carbon atom(s), such as methyl, ethyl, propyl, isopropyl, butyl, isobutyl, tert-butyl, pentyl, hexyl, octyl and the like. Of these, methyl is preferable.
• alkyl that may substitute a group represented by R 1a (when R 1a is alkyl, it does not become a substituent) is exemplified by those similar to the ones mentioned above.
• Cycloalkyl preferably has 3 to 7 carbon atoms and is exemplified by cyclopropyl, cyclopentyl, cyclohexyl, cycloheptyl and the like. Of these, cyclohexyl is preferable.
• Cycloalkylalkyl is that wherein the cycloalkyl moiety is as mentioned above and the alkyl moiety is preferably a linear or branched chain cycloalkylalkyl having 1 to 3 carbon atom(s) such as cyclopropylmethyl, 2-cyclobutylethyl, 3-cyclopentylpropyl, cyclohexylmethyl, 2-cyclohexylethyl, cycloheptylmethyl and the like. Of these, cyclohexylmethyl is preferable.
• Aryl is preferably that having 6 to 14 carbon atoms, which is preferably phenyl, naphthyl, an ortho-fused bicyclic group having 8 to 10 ring atoms, at least one ring being an aromatic ring (e.g., indenyl group) and the like. Of these, phenyl is preferable.
• arylalkyl the aryl moiety is as mentioned above and the alkyl moiety may be linear or molecular chain and preferably has 1 to 3 carbon atom(s), which is exemplified by benzyl, benzhydryl, phenethyl, 3-phenylpropyl, 1-naphthylmethyl, 2-(1-naphthyl)ethyl, 2-(2-naphthyl)ethyl, 3-(2-naphthyl)propyl and the like. Of these, benzyl is preferable.
• heteroaryl for example, a 5- or 6-membered ring group having carbon and 1-4 heteroatom(s) (oxygen, sulfur or nitrogen), an ortho-fused bicyclic heteroaryl having 8-10 ring atoms derived therefrom, particularly a benz derivative and one derived by fusing propenylene, trimethylene or tetramethylene group with the same, and stable N-oxide thereof and the like can be mentioned.
• heteroarylalkyl the heteroaryl moiety is as mentioned above and the alkyl moiety may be preferably linear or branched chain and preferably has 1 to 3 carbon atom(s).
• alkyl moiety may be preferably linear or branched chain and preferably has 1 to 3 carbon atom(s).
• examples thereof include 2-pyrrolylmethyl, 2-pyridylmethyl, 3-pyridylmethyl, 4-pyridylmethyl, 2-thienylmethyl, 2-(2-pyridyl)ethyl, 2-(3-pyridyl)ethyl, 2-(4-pyridyl)ethyl, 3-(2-pyrrolyl)propyl, 4-imidazolylmethyl and the like.
• 2-pyridylmethyl is preferable.
• the heterocycle contains carbon and at least one nitrogen, may have other heteroatom(s) (oxygen or sulfur), may preferably have 1-2 nitrogen atom(s) or oxygen atom(s), and is saturated or unsaturated. It includes not only monocycle but also spiro ring, and is preferably a 4- to 7-membered monocycle ring group or a 10- or 11-membered ring group as a spiro ring.
• heterocycle for example, azetidinyl, pyrrolidinyl, piperidino, piperazinyl, morpholino, 1,4-diazepanyl, 1,2,5,6-tetrahydropyridyl, thiomorpholino, oxothiomorpholino, dioxothiomorpholino, 3-azapyrro[5,5]undecyl, 1,3,8-triazaspiro[4,5]decyl and the like can be mentioned.
• piperidino and piperazinyl are preferable.
• the aromatic ring optionally having substituents may be substituted or condensed.
• the aromatic ring of the aromatic ring optionally having substituents is exemplified by benzene ring, pyridine ring and the like, with preference given to benzene ring.
• the aromatic ring may have one or more of the following substituents, and the substituent is preferably cyano or trifluoromethyl.
• Concrete examples of the condensed ring include indolinyl, isoindolinyl, 1,2,3,4-tetrahydroquinolyl, 1,2,3,4-tetrahydroisoquinolyl, phthalimide, indolyl and the like. Of these, indolinyl and 1,2,3,4-tetrahydroquinolyl are preferable.
• the alkoxy represented by R 13 , R 14 , R 18 , R 19 , R 27 and R 28 preferably has 1 to 8 carbon atom(s) and may be linear or branched chain. Examples thereof include methoxy, ethoxy, propoxy, isopropoxy, butoxy, pentyloxy, hexyloxy, octyloxy and the like. Of these, methoxy and isopropoxy are preferable.
• alkoxycarbonyl that may substitute the carbon atom is exemplified by alkoxycarbonyl wherein the alkoxy moiety is similar to the one mentioned above.
• alkoxy that may substitute a group represented by R 1a is exemplified by those similar to the above-mentioned ones.
• the halogen represented by R 3a , R 4a , R 13 , R 14 , R 18 , R 19 , R 20 , R 21 , R 22 , R 23 , R 24 , R 25 or R 26 is exemplified by chlorine, bromine, fluorine and iodine. Of these, chlorine and fluorine are preferable.
• halogen that may substitute a group represented by R 1a is exemplified by those similar to the above-mentioned ones.
• the haloalkyl represented by R 2a , R 3a , R 4a , R 5a , R 6a , R 7a , R 8b , R 9 , R 9a , R 10a , R 11a , R 12a , R 13a , R 14a , R 17 , R 17a , R 20 , R 21 , R 22 , R 23 , R 24 , R 25 , R 26 , R 27 or R 28 is alkyl substituted by one or more halogen(s), wherein halogen and alkyl are as defined above. Examples thereof include trifluoromethyl, 2,2,2-trifluoroethyl, pentafluoroethyl and the like. Of these, trifluoromethyl and 2,2,2-trifluoroethyl are preferable.
• alkyl, cycloalkyl, cycloalkylalkyl, aryl, arylalkyl, heteroaryl, heteroarylalkyl and heterocycle represented by X′, R 2 , R 3a , R 4a , R 5a , R 6a , R 7a , R 8b , R 9a , R 10a , R 11a , R 12a , R 13a and R 14a may be each substituted by one or more substituent(s) shown in the following.
• halogen is excluded from the substituents of alkyl for R 3a , R 4a , R 5a , R 6a , R 7a , R 8b , R 9a , R 10a , R 11a , R 12a , R 13a or R 14a ), a hydroxyl group, nitro, cyano, trifluoromethyl, alkyl (except alkyl as a substituent for the above-mentioned alkyl), alkoxy, alkylthio, formyl, acyloxy, oxo, phenyl, 2-pyridyl, 3-pyridyl, 4-pyridyl (except phenyl, 2-, 3-, 4-pyridyl as a substituent for alkyl), 4-piperidinyl, 2-morpholinoethyl, 3-picolyl, arylalkyl, —COOR a , —CH 2 COOR a , —CH 2 COOR a , —
• substituents may further have substituent(s).
• substituent(s) such as 4-cyanophenyl, 4-chlorophenyl, 4-methoxyphenyl, 5-cyano-2-pyridyl, 1-ethoxycarbonyl-4-piperidinyl and the like can be mentioned.
• the alkoxy preferably has 1 to 8 carbon atom(s) and may be linear or branched chain. Examples thereof include methoxy, ethoxy, propoxy, butoxy, pentyloxy, hexyloxy, octyloxy and the like.
• the alkylthio preferably has 1 to 8 carbon atom(s) and may be linear or branched chain. Examples thereof include methylthio, ethylthio, propylthio, butylthio, pentylthio, hexylthio, octylthio and the like.
• the acyloxy preferably has 1 to 8 carbon atom(s) and may be linear or branched chain. Examples thereof include formyloxy, acetyloxy, propionyloxy, butyryloxy, valeryloxy, pivaloyloxy, hexanoyloxy, benzoyloxy and the like.
• R a -R n denotes hydrogen, alkyl (as mentioned above) or arylalkyl (as mentioned above).
• R b and R c , R e and R f , R i and R j and R l , and R m of —NR b R c , —NR e R f , —NR i R j and —NR l R m may be bonded to each other to form a heterocycle having carbon and at least one nitrogen and optionally having other heteroatom(s) (oxygen or sulfur), preferably a heterocycle having 1 or 2 nitrogen atom(s) or oxygen atom(s), where this heterocycle is optionally condensed with an aromatic ring optionally having substituent(s) (as mentioned above, and may be substituted by the substituents recited as the substituents for the aforementioned heterocycle), and —NR e R f can also show heteroaryl having ⁇ O (e.g., 2-pyrroli
• T 1 -T 4 shows a hydrogen atom, alkyl, cycloalkyl, cycloalkylalkyl, aryl, arylalkyl, heteroaryl, heteroarylalkyl or haloalkyl, which may be substituted by the substituents recited as the substituents for the aforementioned alkyl, cycloalkyl, cycloalkylalkyl, aryl and arylalkyl.
• pyrimidinyl represented by R 1a 1-pyrimidinyl, 2-pyrimidinyl, 4-pyrimidinyl and 5-pyrimidinyl can be mentioned.
• Y′ is preferably a substituent represented by the above-mentioned formula (II-a), (II-b), (II-c) or (II-d), more preferably phenylamino optionally having substituent(s), 2-pyridylamino optionally having substituent(s) or 4-(1-isoquinolyl)-1-piperazinyl optionally having substituent(s).
• Y is preferably a sulfur atom and X′ is preferably a hydrogen atom.
• compound (I) wherein X is represented by the formula (I-b), compound (I) can exist in an optically active form or as a diastereomer mixture due to an asymmetric carbon bonded with the formula:
• the compound (I) can show polymorphism, and can exist as more than one tautomers.
• the present invention encompasses any stereoisomer, optical isomer, polymorph, tautomer, any mixture thereof and the like mentioned above.
• inorganic acid addition salts e.g., salts with hydrochloric acid, hydrobromic acid, hydriodic acid, sulfuric acid, nitric acid, phosphoric acid and the like
• organic acid addition salts e.g., salts with methanesulfonic acid, benzenesulfonic acid, p-toluenesulfonic acid, formic acid, acetic acid, trifluoroacetic acid, oxalic acid, citric acid, malonic acid, fumaric acid, glutaric acid, adipic acid, maleic acid, tartaric acid, succinic acid, mandelic acid, malic acid, pantothenic acid, methylsulfuric acid and the like), salts with amino acid (e.g., salts with glutamic acid, aspartic acid and the like) and the like can be mentioned.
• inorganic acid addition salts e.g., salts with hydrochloric acid, hydrobromic acid, hydri
• the thiazolidine derivative of the present invention can be produced according to the following method.
• P 1 and P 2 are amino acid-protecting groups (e.g., tert-butoxycarbonyl (Boc), benzyloxycarbonyl (Cbz), (9H-fluorein-9-yl)methyloxycarbonyl (Fmoc) etc.) or solid phase carriers (e.g., Wang resin via carbonyl group), Hal is halogen (preferably fluorine) and other symbols are as defined above.
• amino acid-protecting groups e.g., tert-butoxycarbonyl (Boc), benzyloxycarbonyl (Cbz), (9H-fluorein-9-yl)methyloxycarbonyl (Fmoc) etc.
• Solid phase carriers e.g., Wang resin via carbonyl group
• Hal is halogen (preferably fluorine) and other symbols are as defined above.
• Step a Step for Reacting Compound (III-a) with Compound (IV-a) to Give Amide Compound (V-a)
• condensation agent to activate carboxylic acid of compound (III-a) for example, dicyclohexylcarbodiimide (DCC), N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide (EDC) or hydrochloride thereof, 2-ethoxy-1-ethoxycarbonyl-1,2-dihydroxyquinoline (EEDQ), carbodiimidazole (CDI), diethylphosphoryl cyanide, benzotriazol-1-yloxytripyrrolidinophosphonium hexafluorophosphate (PyBOP), diphenylphosphoryl azide (DPPA), isobutyl chloroformate, diethylacetyl chloride, trimethylacetyl chloride and the like can be mentioned, with preference given to EDC.
• DCC dicyclohexylcarbodiimide
• EEDQ N-(3-dimethylaminopropyl)-N′-ethylcarbodi
• condensation agents are used alone, or in combination with an additive such as N-hydroxysuccinimide (HONSu), hydroxybenzotriazole (HOBT), 3-hydroxy-4-oxo-3,4-dihydro-1,2,3-benzotriazine (HOOBT), 4-dimethylaminopyridine (DMAP) and the like, preferably HOBT.
• an additive such as N-hydroxysuccinimide (HONSu), hydroxybenzotriazole (HOBT), 3-hydroxy-4-oxo-3,4-dihydro-1,2,3-benzotriazine (HOOBT), 4-dimethylaminopyridine (DMAP) and the like, preferably HOBT.
• the amount of compound (IV-a) to be used is generally 90-300 mol %, preferably 100-150 mol %, relative to compound (III-a).
• the amount of the condensation agent to be used is generally 100-300 mol %, preferably 100-200 mol %, relative to compound (III-a).
• the amount of the additive to be used is generally 100-200 mol %, preferably 100-150 mol %, relative to compound (III-a).
• This reaction is generally carried out in a solvent inert to the reaction, and the inert solvent to be used may be any as long as it is aprotic. Preferred are acetonitrile, tetrahydrofuran, dichloromethane, chloroform, N,N-dimethylformamide (DMF) and the like.
• This condensation is generally conducted at a temperature of ⁇ 30° C. to 80° C., preferably ⁇ 10° C. to 25° C.
• Step b Step for Deprotecting Compound (V-a) to Give Compound (VI-a).
• deprotection can be conducted by a reaction in a solvent inert to the deprotection reaction such as acetonitrile, tetrahydrofuran, 1,4-dioxane, ethyl acetate, methanol, ethanol, dichloromethane, chloroform and the like, using an acid such as hydrogen chloride, trifluoroacetic acid and the like, generally at ⁇ 30° C. to 60° C. for 10 min-24 hr.
• a solvent inert such as acetonitrile, tetrahydrofuran, 1,4-dioxane, ethyl acetate, methanol, ethanol, dichloromethane, chloroform and the like
• an acid such as hydrogen chloride, trifluoroacetic acid and the like
• the amount of the acid to be used is generally 100-3000 mol %, preferably 100-1000 mol %, relative to compound (V-a).
• deprotection can be conducted by subjecting the compound to catalytic hydrogen reduction in a solvent inert to the deprotection reaction such as methanol, ethanol, tetrahydrofuran, 1,4-dioxane, ethyl acetate and the like, in the presence of a catalyst such as palladium and the like, reacting the compound with hydrobromic acid-acetic acid, or, for example, reacting the compound with trifluoroacetic acid or trifluoromethanesulfonic acid, preferably trifluoromethanesulfonic acid in the presence of thioanisole or anisole, in, where necessary, a solvent inert to the deprotection reaction such as dichloromethane, chloroform and the like.
• a solvent inert to the deprotection reaction such as dichloromethane, chloroform and the like.
• the amount of thioanisole or anisole to be used is generally 100-2000 mol %, preferably 100-1000 mol %, relative to compound (V-a).
• the amount of trifluoroacetic acid or trifluoromethanesulfonic acid to be used is generally 100-20000 mol %, preferably 100-10000 mol %, relative to compound (V-a).
• deprotection can be conducted by reacting in, where necessary, a solvent inert to the deprotection such as N,N-dimethylformamide, tetrahydrofuran and the like, using a base such as ammonia, piperidine, morpholine and the like, preferably piperidine, generally at ⁇ 30° C. to 60° C. for 5 min-1 hr.
• a solvent inert such as N,N-dimethylformamide, tetrahydrofuran and the like
• a base such as ammonia, piperidine, morpholine and the like, preferably piperidine, generally at ⁇ 30° C. to 60° C. for 5 min-1 hr.
• the amount of the base to be used is generally 100-2000 mol %, preferably 100-500 mol %, relative to compound (V-a).
• Step c Step for Reacting Compound (VI-a) with Compound (VII-a) to Give Compound (VIII-a).
• the reaction is carried out in the presence of a base such as triethylamine, diisopropylethylamine and the like, preferably diisopropylethylamine, in a solvent inert to the reaction such as N-methyl-2-pyrrolidone, N,N-dimethylformamide, tetrahydrofuran and the like at 0° C. to a temperature near the boiling point of the solvent, preferably 0° C. to 80° C.
• a base such as triethylamine, diisopropylethylamine and the like, preferably diisopropylethylamine
• the amount of compound (VII-a) to be used is generally 100-500 mol %, preferably 100-200 mol %, relative to compound (VI-a).
• the amount of the base to be used is generally 100-500 mol %, preferably 120-300 mol %, relative to compound (VI-a).
• Step d Step for Deprotecting Compound (VIII-a) to Give Compound (I-a1).
• the same reaction conditions as in Step b are employed.
• the solid phase carrier P 1 is Wang resin via carbonyl group
• the solid phase carrier can be separated by, for example, reaction in, where necessary, a solvent inert to the reaction such as dichloromethane and the like, and, where necessary, adding an additive such as thioanisole, anisole, phenol, ethylenedithiol and the like and using trifluoroacetic acid as a solvent generally at a temperature near room temperature for 1-24 hr.
• L is a leaving group (e.g., halogen, tosylate (OTs), mesylate (OMs), triflate (OTf) and the like), and other symbols are as defined above.
• OTs tosylate
• OMs mesylate
• OTf triflate
• Step e Step for Reacting Compound (X-a) or Compound (X-b) with Compound (IX-a) to Give Compound (XI-a) or Compound (XI-b).
• the reaction is carried out in the presence of a base such as potassium carbonate, cesium carbonate, sodium carbonate, sodium hydrogencarbonate, triethylamine, diisopropylethylamine and the like, preferably potassium carbonate, in a solvent inert to the reaction such as N-methyl-2-pyrrolidone, N,N-dimethylformamide, tetrahydrofuran and the like, at 0° C.—a temperature near the boiling point of solvent, preferably 0° C. to 80° C.
• a base such as potassium carbonate, cesium carbonate, sodium carbonate, sodium hydrogencarbonate, triethylamine, diisopropylethylamine and the like, preferably potassium carbonate
• a solvent inert such as N-methyl-2-pyrrolidone, N,N-dimethylformamide, tetrahydrofuran and the like
• the amount of compound (X-a) or compound (X-b) to be used is generally 100-500 mol %, preferably 100-200 mol %, relative to compound (IX-a).
• the amount of the base to be used is generally 100-500 mol %, preferably 100-300 mol %, relative to compound (IX-a).
• Step f Step for Deprotecting Compound (XI-a) or (XI-b) to Give Compound (I-a2) or (I-a3). Conducted According to a Method Similar to that in Step b.
• Step g Step for Reacting Compound (XII-a) with Compound (XIII) to Give Compound (XIV).
• the reaction is carried out in the presence of a base such as potassium carbonate, cesium carbonate, sodium carbonate, sodium hydrogencarbonate, triethylamine, diisopropylethylamine and the like, preferably diisopropylethylamine, in a solvent inert to the reaction such as N-methyl-2-pyrrolidone, N,N-dimethylformamide, tetrahydrofuran and the like, at 0° C.—a temperature near the boiling point of solvent, preferably 0° C. to 80° C.
• a base such as potassium carbonate, cesium carbonate, sodium carbonate, sodium hydrogencarbonate, triethylamine, diisopropylethylamine and the like, preferably diisopropylethylamine
• the amount of compound (XIII) to be used is generally 100-500 mol %, preferably 100-200 mol %, relative to compound (XII-a).
• the amount of the base to be used is generally 100-500 mol %, preferably 100-300 mol %, relative to compound (XII-a).
• Step h Step for Deprotection of Compound (XIV) to Give Compound (I-a1). Conducted According to a Method Similar to that in Step b.
• Step i Step for Oxidation of Compound (XV) to Give Compound (XVI).
• a method using a pyridine sulfur trioxide complex and dimethyl sulfoxide at room temperature is preferable, but other useful methods include, for example, a method using alkaline potassium permanganate solution; a method using oxalyl chloride, dimethyl sulfoxide and tertiary amine; a method using acetic anhydride and dimethyl sulfoxide; a method using dichloroacetic acid as a catalyst, DCC or EDC and dimethyl sulfoxide; a method using a chromium (VI) oxide pyridine complex in dichloromethane; a method using aqueous sodium hypochlorite solution in ethyl acetate or toluene with TEMPO free radical as a catalyst in the presence of sodium bromide and the like.
• a method using alkaline potassium permanganate solution a method using oxalyl chloride, dimethyl sulfoxide and tertiary amine
• Step j Step for Reacting Compound (XVI) with Compound (XIII) and Then Reducing to Give Compound (XIV).
• This reaction may be carried out in the presence of a reducing agent such as sodium borohydride, sodium cyanoborohydride, sodium triacetoxyborohydride and the like, preferably sodium triacetoxyborohydride, in a solvent inert to the reaction such as methanol, ethanol, dichloromethane, 1,2-dichloroethane, chloroform, tetrahydrofuran, acetonitrile, 1,4-dioxane and the, like, using, where necessary, an acidic catalyst such as acetic acid, p-toluenesulfonic acid, a boron trifluoride diethyl ether complex and the like, preferably acetic acid, generally at 0° C. to 100° C. for 10 min-10 hr.
• a reducing agent such as sodium borohydride, sodium cyanoborohydride, sodium triacetoxyborohydride and the like, preferably sodium triacetoxyborohydride
• a solvent inert such
• the amount of compound (XIII) to be used is generally 100-300 mol %, preferably 100-200 mol %, relative to compound (XVI).
• the amount of the reducing agent to be used is generally 100-500 mol %, preferably 100-300 mol %, relative to compound (XVI).
• Step k Step for Deprotecting Compound (XIV) to Give Compound (I-a1). Conducted According to a Method Similar to that in Step b.
• the starting material compound (XII-a) in Scheme 3 can be synthesized by, as shown in Scheme 5, condensing carboxylic acid compound represented by the formula (XVII) and compound (IV-a) according to the same method as in Step a, halogenating a hydroxyl form represented by the formula (XV), or sulfonylating the hydroxyl form using methanesulfonyl chloride, p-toluenesulfonyl chloride, trifluoromethanesulfonic anhydride and the like.
• a compound represented by the starting material compound (XII-a) wherein L is halogen can be also produced by reacting a compound represented by the formula (IX-a) with sodium nitrite or nitrite in the presence of the corresponding alkali metal halide salt.
• the hydroxyl form represented by the formula (XV) can be produced by condensing the corresponding N-protected hydroxyamino acid (XVIII) and compound (IV-a) according to the same method as in Step a, or subjecting dipeptide compound (XIX) comprising N-protected aspartic acid, N-protected glutamic acid, 2-protected aminoadipic acid, 2-protected aminopimeric acid and compound (IV-a) to reduction reaction using lithium borohydride sodium and the like.
• R 15 is alkyl (as defined above) or arylalkyl (as defined above) and other symbols are as defined above.
• R 35 is an amino-protecting group (e.g., tert-butoxycatbonyl (Boc), benzyloxycarbonyl (Cbz)), —OSO 2 R 37 is a leaving group (e.g., tosylate (OTs), mesylate (OMs), triflate (OTf)), Hal is halogen, and other symbols are as defined above.
• R 35 is an amino-protecting group (e.g., tert-butoxycatbonyl (Boc), benzyloxycarbonyl (Cbz)), —OSO 2 R 37 is a leaving group (e.g., tosylate (OTs), mesylate (OMs), triflate (OTf)), Hal is halogen, and other symbols are as defined above.
• Step f′ Step for Subjecting a Hydroxyl Group of Compound (XIII-a) to Sulfonylation to Give Compound (XIV-a).
• This reaction is carried out in the presence of a base such as pyridine, triethylamine and the like, using sulfonyl chloride such as p-toluenesulfonyl chloride, methanesulfonyl chloride, trifluoromethanesulfonyl chloride and the like, preferably methanesulfonyl chloride, in a solvent inert to the reaction such as dichloromethane, 1,2-dichloroethane and the like at a temperature of generally ⁇ 30° C. to 60° C. for 10 min-24 r.
• a base such as pyridine, triethylamine and the like
• sulfonyl chloride such as p-toluenesulfonyl chloride, methanesulfonyl chloride, trifluoromethanesulfonyl chloride and the like, preferably methanesulfonyl chloride
• the amount of sulfonyl chloride to be used is generally 100-300 mol %, preferably 100-200 mol %, relative to compound (XIII-a).
• Step g′ Step for Subjecting Compound (XIV-a) to Azidation to Give Compound (XV-a).
• reaction is carried out using a metal azide, such as sodium azide in a solvent such as N,N-dimethylformamide and the like at a temperature of generally 0° C. to 120° C. for 30 min to 24 hr.
• a metal azide such as sodium azide
• a solvent such as N,N-dimethylformamide and the like
• the amount of metal azide to be used is generally 100-300 mol %, preferably 100-150 mol %, relative to compound (XIV-a).
• Step h′ Step for Directly Obtaining Compound (XV-a) from Compound (XIII-a).
• the reaction is carried out in the presence of phosphines such as triphenylphosphine, tributylphosphine and the like and diazodicarboxylic acid diester, using an azidating reagent such as hydrogen azide, DPPA, zinc azide bispyridine complex salt and the like, preferably DPPA, in a solvent inert to the reaction such as toluene, tetrahydrofuran and the like at a reaction temperature of generally ⁇ 30° C. to 100° C.
• phosphines such as triphenylphosphine, tributylphosphine and the like and diazodicarboxylic acid diester
• an azidating reagent such as hydrogen azide, DPPA, zinc azide bispyridine complex salt and the like, preferably DPPA
• a solvent inert to the reaction such as toluene, tetrahydrofuran and the like at a reaction temperature of generally ⁇ 30° C.
• the amount of phosphines to be used is generally 100-300 mol %, preferably 100-200 mol %, relative to compound (XIII-a).
• the amount of diazodicarboxylic acid diester to be used is generally 100-300 mol %, preferably 100-200 mol %, relative to compound (XIII-a).
• the amount of the azidating reagent to be used is generally 100-300 mol %, preferably 100-200 mol %, relative to compound (XIII-a).
• Step i′ Step for Reducing Compound (XV-a) to Give Compound (XVI-a).
• Step j′ Step for Reacting Compound (XVI-a) with Compound (XVII-a) to Give Compound (XIX-a).
• the reaction is carried out in the presence of a base such as triethylamine, diisopropylethylamine and the like, preferably diisopropylethylamine, in a solvent inert to the reaction such as N-methyl-2-pyrrolidone, N,N-dimethylformamide, tetrahydrofuran and the like at 0° C. to a temperature near the boiling point of solvent, preferably 0° C. to 80° C.
• a base such as triethylamine, diisopropylethylamine and the like, preferably diisopropylethylamine
• the amount of compound (XVII-a) to be used is generally 100-500 mol %, preferably 100-200 mol %, relative to compound (XVI-a).
• the amount of the base to be used is generally 200-1000 mol %, preferably 200-500 mol %, relative to compound (XVI-a).
• Step k′ Step for Reacting Compound (XVI-a) with Compound (XVIII-a) and then Reducing to Give Compound (XIX-a).
• This reaction may be carried out in the presence of a reducing agent such as sodium borohydride, sodium cyanoborohydride, sodium triacetoxyborohydride and the like, preferably sodium triacetoxyborohydride, in a solvent inert to the reaction such as methanol, ethanol, dichloromethane, chloroform, 1,2-dichloroethane, tetrahydrofuran, acetonitrile, 1,4-dioxane and the like, using, where necessary, an acidic catalyst, such as acetic acid, p-toluenesulfonic acid, boron trifluoride diethyl ether complex and the like at a temperature of generally 0° C. to 100° C. for 10 min-20 hr.
• a reducing agent such as sodium borohydride, sodium cyanoborohydride, sodium triacetoxyborohydride and the like, preferably sodium triacetoxyborohydride
• the amount of compound (XVIII-a) to be used is generally 100-300 mol %, preferably 100-200 mol %, relative to compound (XVI-a).
• the amount of the reducing agent to be used is generally 200-1000 mol %, preferably 200-500 mol %, relative to compound (XVI-a).
• Step m Step for Oxidation of Compound (XIII-a) to Give Compound (XX).
• a method using a pyridine sulfur trioxide complex and dimethyl sulfoxide at room temperature is preferable, but other useful methods include, for example, a method using alkaline potassium permanganate solution; a method using oxalyl chloride, dimethyl sulfoxide and tertiary amine; a method using acetic anhydride and dimethyl sulfoxide; a method using dichloroacetic acid as a catalyst, DCC or EDC and dimethyl sulfoxide; a method using a chromium (IV) oxide pyridine complex in dichloromethane; a method using aqueous sodium hypochlorite solution in ethyl acetate or toluene with TEMPO free radical as a catalyst in the presence of sodium bromide and the like.
• Step n Step for Reacting Compound (XX) with Compound (XXI) and Then Reducing to Give Compound (XIX-a).
• This reaction may be carried out in the presence of a reducing agent such as sodium borohydride, sodium cyanoborohydride, sodium triacetoxyborohydride and the like, preferably sodium triacetoxyborohydride, in a solvent inert to the reaction such as methanol, ethanol, dichloromethane, chloroform, 1,2-dichloroethane, tetrahydrofuran, acetonitrile, 1,4-dioxane and the like, using, where necessary, an acidic catalyst, such as acetic acid, p-toluenesulfonic acid, boron trifluoride diethyl ether complex and the like at a temperature of generally 0° C. to 100° C. for 10 min-20 hr.
• a reducing agent such as sodium borohydride, sodium cyanoborohydride, sodium triacetoxyborohydride and the like, preferably sodium triacetoxyborohydride
• the amount of compound (XXI) to be used is generally 100-300 mol %, preferably 100-200 mol %, relative to compound (XX).
• the amount of the reducing agent to be used is generally 100-500 mol %, preferably 100-300 mol %, relative to compound (XX).
• the compound (XXI) can be synthesized according to a known method.
• Step o Step for Deprotection of Compound (XIX-a) to Give Compound (I-b1).
• the protecting group R 35 is a Boc group in this reaction
• deprotection can be conducted by reaction in a solvent such as acetonitrile, tetrahydrofuran, 1,4-dioxane, ethyl acetate, methanol, ethanol, dichloromethane, chloroform and the like, using an acid such as hydrogen chloride, trifluoroacetic acid and the like generally at ⁇ 30° C. to 60° C. for 10 min-24 hr.
• the amount of the acid to be used is generally 100-3000 mol %, preferably 100-1000 mol %, relative to compound (XIX-a).
• deprotection can be conducted by catalytic hydrogen reduction in a solvent inert to the deprotection such as methanol, ethanol, tetrahydrofuran, 1,4-dioxane, ethyl acetate and the like, in the presence of a catalyst such as palladium and the like, reaction with hydrobromic acid-acetic acid, or, for example, reaction with trifluoroacetic acid or trifluoromethanesulfonic acid, preferably trifluoromethanesulfonic acid in, where necessary, a solvent inert to the deprotection reaction such as dichloromethane, chloroform and the like, in the presence of thioanisole or anisole.
• a solvent inert to the deprotection reaction such as dichloromethane, chloroform and the like, in the presence of thioanisole or anisole.
• the amount of thioanisole or anisole to be used is generally 100-2000 mol %, preferably 100-1000 mol %, relative to compound (XIX-a).
• the amount of trifluoroacetic acid or trifluoromethanesulfonic acid to be used is generally 100-20000 mol %, preferably 100-10000 mol %, relative to compound (XIX-a).
• R 40 is alkyl such as methyl, ethyl and the like, benzyl and the like and other symbols are as defined above.
• Step v is the same as the method of conversion from compound (XIII-a) to compound (XIX-a) as shown in Scheme 6.
• Step w Step for Deprotection of Ester-Protected Carboxyl Group of Compound (XXXIII) to Give Compound (XXXIV).
• deprotection reaction can be conducted by, for example, hydrolysis under alkaline conditions with sodium hydroxide, potassium hydroxide, sodium carbonate, potassium carbonate and the like, preferably sodium hydroxide, or when R 40 is benzyl, catalytic hydrogenation in the presence of platinum, palladium and the like, in a solvent inert to the reaction such as ethanol, ethanol and the like.
• Step x Step for Reacting Compound (XXXV) with Compound (XXXIV) to Give Compound (XIX-a).
• the condensation agent shown in Step a preferably EDC
• the reaction is carried out using a condensation agent alone, or in combination with an additive shown in Step a, preferably HOBT, in a solvent inert to the reaction such as acetonitrile, tetrahydrofuran, dichloromethane, chloroform, N,N-dimethylformamide and the like generally at a temperature of ⁇ 30° C. to 80° C., preferably ⁇ 10° C. to 25° C.
• the amount of compound (XXXV) to be used is generally 90-300 mol %, preferably 100-150 mol %, relative to compound (XXXIV).
• the amount of the condensation agent to be used is generally 100-300 mol %, preferably 100-200 mol %, relative to compound (XXXIV).
• the amount of the additive to be used is generally 100-200 mol %, preferably 100-150 mol %, relative to compound (XXXIV).
• each symbol is as defined above, as a starting material according to a method similar to the above-mentioned method.
• This reaction is carried out using diphosphorus pentaoxide, phosphorus oxychloride-imidazole, trifluoroacetic anhydride, p-toluenesulfonyl chloride-pyridine and the like as a dehydrating agent in an inert solvent such as dichloromethane, pyridine and the like.
• the thiazolidine derivative of the formula (I) of the present invention thus produced can be obtained at any purity by appropriately applying known separation and purification means, such as concentration, extraction, chromatography, re-precipitation, recrystallization and the like.
• the thiazolidine derivative of the formula (I) can be converted to an acid addition salt with an inorganic acid such as hydrochloric acid, hydrobromic acid, hydriodic acid, sulfuric acid, nitric acid, phosphoric acid and the like or an organic acid such as methanesulfonic acid, benzenesulfonic acid, p-toluenesulfonic acid, formic acid, acetic acid, trifluoroacetic acid, oxalic acid, citric acid, malonic acid, fumaric acid, glutaric acid, adipic acid, maleic acid, tartaric acid, succinic acid, mandelic acid, malic acid, pantothenic acid, methylsulfuric acid and the like. It can be also present as a solvate such as hydrate and the like.
• an inorganic acid such as hydrochloric acid, hydrobromic acid, hydriodic acid, sulfuric acid, nitric acid, phosphoric acid and the like
• the compound of the formula (I) and a pharmacologically acceptable salt thereof (pharmaceutically acceptable salt) of the present invention exhibit a superior DPP-IV inhibitory activity in mammals (e.g., human, dog, cat, rat etc.).
• the compound (I) and a pharmacologically acceptable salt thereof (pharmaceutically acceptable salt) of the present invention exhibit a potent DPP-IV inhibitory activity as demonstrated in the experiment to be mentioned below, they are useful as DPP-IV inhibitors and useful for the prophylaxis or treatment of various diseases in which DPP-IV is involved and the like, including the prophylaxis or treatment of diseases in which GLP-1 is considered to be involved (e.g., diabetes, obesity etc.) and the like.
• a disease in which DPP-IV is involved is, for example, diabetes, obesity and the like.
• the compound (I) of the present invention can be administered simultaneously with other therapeutic drugs for diabetes, therapeutic drugs for diabetic complications, anti-hyperlipidemia agents, hypotensive agents and the like to a single subject, or in a staggered manner to a single subject.
• the ratio of addition can be appropriately determined according to the administration subject, age and body weight of the administration subject, condition, administration time, dosage form, administration method, combination and the like.
• compound (I) and a pharmacologically acceptable salt thereof (pharmaceutically acceptable salt) of the present invention are used as the aforementioned pharmaceutical agents, they can be administered orally or parenterally as they are or in the form of powder, granule, tablet, capsule, injection and the like after admixing with appropriate pharmacologically acceptable carrier, excipient, diluent and the like.
• the above-mentioned preparation contains an effective amount of compound (I) or a pharmacologically acceptable salt (pharmaceutically acceptable salt).
• the dose of said compound (I) or a pharmacologically acceptable salt (pharmaceutically acceptable salt) varies depending on the administration route, target disease, condition, body weight and age of patient, and the compound to be used, and can be determined appropriately depending on the administration object.
• the dose is generally 0.01-1000 mg/kg body weight/day, preferably 0.05-500 mg/kg body weight/day, which is preferably administered in once to several times a day in divided doses.
• 1 H-NMR was measured at 300 MHz unless particularly indicated.
• the chemical shifts of 1 H-NMR was measured using tetramethylsilane (TMS) as the internal standard and expressed as relative delta ( ⁇ ) value in parts per million (ppm).
• TMS tetramethylsilane
• ⁇ relative delta
• ppm parts per million
• obvious multiplicity is shown using s (singlet), d (doublet), t (triplet), q (quartet), quint (quintet), m (multiplet), dd (doublet of doublets), td (triplet of doublets), brs (broad singlet) and the like in hertz (Hz).
• Thin layer chromatography was performed using silica gel manufactured by Merck and column chromatography was performed using silica gel manufactured by Fuji Silysia Chemical Ltd. Purification by HPLC was performed using Develosil Combi-RP. For drying organic solutions in extraction, anhydrous sodium sulfate or anhydrous magnesium sulfate was used unless particularly indicated.
• N- ⁇ -Benzyloxycarbonyl-N- ⁇ -tert-butoxycarbonyl-L-lysine (19 g) was dissolved in dichloromethane (200 mL), and thiazolidine (3.91 mL), HOBT monohydrate (11.5 g) and EDC hydrochloride (14.4 g) were added successively. The mixture was stirred overnight. The reaction solution was concentrated under reduced pressure, and a 10% aqueous citric acid solution was added. The mixture was extracted with ethyl acetate. The extract solution was washed successively with a 10% aqueous citric acid solution, a saturated aqueous sodium hydrogen carbonate solution and saturated brine and dried. The solvent was evaporated under reduced pressure to give 3-[(S)-6-(tert-butoxycarbonyl)amino-2-(benzyloxycarbonyl)aminohexanoyl]-1,3-thiazolidine.
• N-tert-Butoxycarbonyl-L-trans-4-hydroxyproline (69.4 g) and thiazolidine (29.4 g) were dissolved in DMF (300 mL), and HOBT (50.5 g) and EDC hydrochloride (63.3 g) were added successively. The mixture was stirred at room temperature for 18 hr. The reaction solution was concentrated and saturated brine and a saturated aqueous sodium hydrogen carbonate solution were added to the concentrate. The mixture was extracted with ethyl acetate.
• Example 7(2) In the same manner as in Example 7(2) and using the resin (700 mg) of Example 7(1) and 2-fluorobenzonitrile (0.277 mL), the title compound (3 mg) was obtained.
• Example 7(2) In the same manner as in Example 7(2) and using the resin (700 mg) of Example 7(1) and 4-fluorobenzonitrile (0.277 mL), the title compound (6.8 mg) was obtained.
• N- ⁇ -benzyloxycarbonyl-N- ⁇ -tert-butoxycarbonyl-L-lysine 8.60 g was dissolved in formic acid (50 mL), and the mixture was stirred overnight at room temperature. The reaction solution was concentrated under reduced pressure, and the residue was dissolved in acetic acid (100 mL), and sodium acetate (1.85 g) and sodium nitrite (4.68 g) were added. The mixture was stirred overnight at 40° C. The reaction solution was concentrated under reduced pressure, and water was added to the residue. The mixture was extracted with chloroform. The extract solution was dried and concentrated under reduced pressure to give (S)-6-acetoxy-2-(benzyloxycarbonyl)aminohexane acid (3.52 g).
• the extract solution- was washed successively with a saturated aqueous sodium hydrogen carbonate solution and saturated brine and dried.
• the solvent was evaporated under reduced pressure.
• the residue was purified by silica gel chromatography to give 1-tert-butoxycarbonyl-4-[1-(2-fluorophenyl)-3-methyl-5-pyrazolyl]piperazine (0.640 g) as an oil.
• Example 35(2) In the same manner as in Example 35(2) and using the compound (5.0 g) of Example 35(1) and 4-chlorophenylhydrazine hydrochloride (3.5 g), 1-tert-butoxycarbonyl-4-[1-(4-chlorophenyl)-3-methyl-5-pyrazolyl]piperazine (2.2 g) was obtained as a brown solid.
• Example 35(2) In the same manner as in Example 35(2) and using the product (5.0 g) of Example 35(1) and 4-cyanophenylhydrazine hydrochloride (3.3 g), 1-tert-butoxycarbonyl-4-[1-(4-cyanophenyl)-3-methyl-5-pyrazolyl]piperazine (2.7 g) was obtained as a pale-yellow solid.
• Example 35(1) The product (3.92 g) of Example 35(1) was dissolved in ethanol (200 mL) and 2-hydrazinopyridine (1.58 g), methanesulfonic acid (0.094 mL) and molecular sieves 3A (10 g) were added at room temperature. The mixture was stirred for 18 hr. The molecular sieves were filtered off and pyridine (4 mL) was added to the filtrate. The solvent was evaporated under reduced pressure. The residue was dissolved in pyridine (200 mL) and phosphorus oxychloride (3.0 mL) was added at room temperature. The mixture was stirred for 18 hr.
• reaction solution was concentrated under reduced pressure, and a saturated aqueous sodium hydrogen carbonate solution was added to the residue.
• the mixture was extracted with ethyl acetate.
• the extract solution was washed with saturated brine, dried and concentrated under reduced pressure.
• the residue was purified by silica gel chromatography to give 1-tert-butoxycarbonyl-4-[3-methyl-1-(2-pyridyl)-5-pyrazolyl]piperazine (230 mg).
• the reaction solution was concentrated under reduced pressure, and a saturated aqueous sodium hydrogen carbonate solution was added to the residue.
• the mixture was extracted with ethyl acetate.
• the extract solution was washed successively with water and saturated brine, dried and concentrated under reduced pressure.
• the residue was purified by silica gel chromatography to give 1-tert-butoxycarbonyl-4-[1-(5-cyano-2-pyridyl)-3-methyl-5-pyrazolyl]piperazine (1.3 g) as a pale-yellow solid.
• reaction solution was poured onto ice water (3 L), and the precipitated solid was collected by filtration.
• the solid was dissolved in ethyl acetate and the mixture was washed successively with a saturated aqueous sodium hydrogen carbonate solution, water and saturated brine, dried and concentrated under reduced pressure to give a mixture (31.7 g) containing 4-trifluoromethyl-2-hydroxy-8-methoxyquinoline as a pale-brown solid.
• reaction solution was poured onto ice water (3 L) and the precipitated solid was collected by filtration.
• the solid was dissolved in ethyl acetate and washed successively with a saturated aqueous sodium hydrogen carbonate solution, water and saturated brine, dried and concentrated under reduced pressure to give a mixture (31.7 g) containing 2-trifluoromethyl-4-hydroxy-8-methoxyquinoline as a pale-brown solid.
• Example 50(3) The product (4.86 g) of Example 50(3) was dissolved in dichloromethane and boron tribromide (7.39 mL) was added at ⁇ 78° C. The mixture was stirred at room temperature for 3 hr. The reaction solution was poured into water and sodium hydrogen carbonate was added to adjust its pH to 8. The mixture was extracted with chloroform. The extract solution was washed with saturated brine, dried and concentrated under reduced pressure to give 1-(2-trifluoromethyl-8-hydroxy-4-quinolyl)piperazine (1.52 g) as a pale-yellow solid.
• Example 51(2) The product (345 mg) of Example 51(2) was dissolved in DMF (3 mL) and potassium carbonate (164 mg) and methanesulfonic acid 2,2,2-trifluoroethyl ester (212 mg) were added. The mixture was stirred at 100° C. for 5 hr. Water was added to the reaction solution, and the mixture was extracted with chloroform. The extract solution was washed with saturated brine, dried and concentrated under reduced pressure.
• reaction solution was poured onto ice, and the mixture was extracted with ethyl acetate.
• the extract solution was washed successively with a saturated aqueous sodium hydrogen carbonate solution, 1 mol/L hydrochloric acid and saturated brine, dried and concentrated under reduced pressure. Ethanol was added to the residue and the precipitate was filtered off. The filtrate was concentrated under reduced pressure to give 2-trifluoromethyl-4-hydroxy-6,8-dimethoxyquinoline (12 g) as an oil.
• Example 56(1) The product (5.2 g) of Example 56(1) was dissolved in methanol (25 mL) and triethylamine (5.6 mL) and dibenzoylacetone (3.2 g) were added. The mixture was stirred at room temperature for 3 days. The reaction solution was concentrated under reduced pressure and saturated brine was added. The mixture was extracted with ethyl acetate. The extract solution was washed successively with a 10% aqueous citric acid solution, a saturated aqueous sodium hydrogen carbonate solution and saturated brine, dried and concentrated under reduced pressure. The residue was purified by silica gel chromatography to give 1-ethoxycarbonyl-4-(3-methyl-5-phenyl-1-pyrazolyl)piperidine (3.74 g).
• Example 56(1) The product (2.6 g) of Example 56(1) was dissolved in methanol (10 mL) and triethylamine (2.8 mL) and dibenzoylmethane (2.2 g) were added. The mixture was stirred at 60° C. one day. The reaction solution was concentrated under reduced pressure and water was added. The precipitated crystals were collected by filtration to give 1-ethoxycarbonyl-4-(3,5-diphenyl-1-pyrazolyl)piperidine (2.71 g) as crystals.
• Isonipecotic acid (19.0 g) was dissolved in water (150 mL) and 1,4-dioxane (300 mL), and a 1 mol/L aqueous sodium hydroxide solution (150 mL) and di-tert-butyl dicarbonate (35.3 g) were added under ice-cooling. The mixture was stirred at room temperature for 3 days. 1,4-Dioxane was evaporated under reduced pressure and a 5% aqueous potassium hydrogen sulfate solution was added to the residue. The precipitated solid was collected by filtration to give 1-tert-butoxycarbonylisonipecotic acid (33.0 g) as a white solid.
• Example 64(2) The product (1.9 g) of Example 64(2) was dissolved in dichloromethane (15 mL) and acetic anhydride (0.500 mL) was added. The mixture was stirred overnight. The reaction solution was concentrated under reduced pressure to give 4-(2-acetylamino-4-trifluoromethylphenyl)amino-1-tert-butoxycarbonylpiperidine.
• Example 66(2) In the same manner as in Example 66(2) and using the product (1.54 g) of Example 66(1) and 2-amino-5-methoxyphenol (1.01 g), 1-benzyloxycarbonyl-4-(6-methoxy-2-benzoxazolyl)piperidine (1.61 g) was obtained as a brown oil.
• the plasma DPP-IV inhibitory activity of human and rat was measured according to the fluorescence assay method and under the conditions of Table 10.
• a DPP-IV specific fluorescent substrate Gly-Pro-MCA (Peptide Institute, Inc.) was used, and reaction solutions having the following compositions containing various concentrations of the test substance were incubated at room temperature for 60 min and the fluorescence intensity (Excitation 360 nm/Emission 465 nm) measured (SPECTRA FLUOR, Tecan Group, Ltd.) was taken as the DPP-IV activity.
• IC 50 values of the plasma DPP-IV inhibitory activity of the present invention as determined according to the above method are shown in Tables 11 and 12. TABLE 11 Example Human plasma DPP-IV Rat plasma DPP-IV compound inhibitory activity inhibitory activity No. IC 50 (nM) IC 50 (nM) 5 28 19 6 34 29 7 15 13 13 66 40 14 29 18 28 35 49 comparison 856 719 compound 1
• comparison compound 1 is encompassed in WO99/61431. As shown in Table 11, the plasma DPP-IV inhibitory activity is not sufficient.
• the compounds of the present invention have a potent DPP-IV inhibitory activity, and are useful for the prophylaxis or treatment of diabetes or the prophylaxis or treatment of obesity.

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