UA94399C2 - Мутантные промоторы аох1 - Google Patents
Мутантные промоторы аох1Info
- Publication number
- UA94399C2 UA94399C2 UAA200710436A UAA200710436A UA94399C2 UA 94399 C2 UA94399 C2 UA 94399C2 UA A200710436 A UAA200710436 A UA A200710436A UA A200710436 A UAA200710436 A UA A200710436A UA 94399 C2 UA94399 C2 UA 94399C2
- Authority
- UA
- Ukraine
- Prior art keywords
- nucleotides
- mutant promoters
- seq
- aox1
- pichia pastoris
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
- C12Y101/03—Oxidoreductases acting on the CH-OH group of donors (1.1) with a oxygen as acceptor (1.1.3)
- C12Y101/03013—Alcohol oxidase (1.1.3.13)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Materials For Medical Uses (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Enzymes And Modification Thereof (AREA)
- Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
- Diaphragms For Electromechanical Transducers (AREA)
Abstract
Мутантный промотор алкогольоксидазы 1 (АОХ1) Pichia pastoris промотора AOX1 Pichia pastoris дикого типа (SEQ ID No. 1), который включает как минимум одну мутацию, выбранную из группы, которая состоит из: а) сайта связывания фактора транскрипции (TFBS), б) нуклеотидов 170-235 (от -784 до -719), нуклеотидов 192-213 (от -762 до -741), 192-210 (от -762 до -744), нуклеотидов 207-209 (от -747 до -745), нуклеотидов 214-235 (от -740 до -719), нуклеотидов 304-350 (от -650 до -604), нуклеотидов 364-393 (от -590 до -561), нуклеотидов 434-508 (от -520 до -446), нуклеотидов 509-551 (от -445 до -403), нуклеотидов 552-560 (от -402 до -394), нуклеотидов 585-617 (от -369 до -337), нуклеотидов 621-660 (от -333 до -294), нуклеотидов 625-683 (от -329 до -271), нуклеотидов 736-741 (от -218 до -213), нуклеотидов 737-738 (от -217 до -216), нуклеотидов 726-755 (от -228 до -199), нуклеотидов 784-800 (от -170 до -154) или нуклеотидов 823-861 (от -131 до -93) Seq ID No. 1 и их комбинации.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AT0030405A AT501955B1 (de) | 2005-02-23 | 2005-02-23 | Mutierte aox1-promotoren |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| UA94399C2 true UA94399C2 (ru) | 2011-05-10 |
Family
ID=36927772
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| UAA200710436A UA94399C2 (ru) | 2005-02-23 | 2006-02-23 | Мутантные промоторы аох1 |
| UAA201012563A UA98604C2 (ru) | 2005-02-23 | 2006-02-23 | Мутантные промоторы аох1 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| UAA201012563A UA98604C2 (ru) | 2005-02-23 | 2006-02-23 | Мутантные промоторы аох1 |
Country Status (21)
| Country | Link |
|---|---|
| US (2) | US9012175B2 (ru) |
| EP (2) | EP2199389B1 (ru) |
| JP (2) | JP5497986B2 (ru) |
| KR (2) | KR101352399B1 (ru) |
| CN (2) | CN101128581B (ru) |
| AT (3) | AT501955B1 (ru) |
| AU (2) | AU2006216094B2 (ru) |
| BR (1) | BRPI0606179B1 (ru) |
| CA (2) | CA2598514C (ru) |
| DE (2) | DE602006018113D1 (ru) |
| DK (2) | DK1851312T5 (ru) |
| ES (2) | ES2358086T3 (ru) |
| HK (2) | HK1148313A1 (ru) |
| HR (2) | HRP20110084T2 (ru) |
| IN (1) | IN2012DN06694A (ru) |
| NZ (2) | NZ560542A (ru) |
| PL (2) | PL1851312T3 (ru) |
| PT (1) | PT1851312E (ru) |
| SI (2) | SI1851312T1 (ru) |
| UA (2) | UA94399C2 (ru) |
| WO (1) | WO2006089329A2 (ru) |
Families Citing this family (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5444323B2 (ja) * | 2008-03-27 | 2014-03-19 | イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー | 高発現ザイモモナス（Ｚｙｍｏｍｏｎａｓ）プロモーター |
| EP2300615A2 (en) * | 2008-07-11 | 2011-03-30 | Novozymes A/S | Pichia pastoris das promoter variants |
| EP2258855A1 (en) | 2009-05-28 | 2010-12-08 | Universität für Bodenkultur Wien | Expression sequences |
| WO2012129036A2 (en) * | 2011-03-22 | 2012-09-27 | Merck Sharp & Dohme Corp. | Promoters for high level recombinant expression in fungal host cells |
| GB201113419D0 (en) * | 2011-08-04 | 2011-09-21 | Fujifilm Diosynth Biotechnologies Uk Ltd | Yeast vector |
| EP2565262A1 (en) * | 2011-08-31 | 2013-03-06 | VTU Holding GmbH | Protein expression |
| EP3508494B1 (en) | 2011-10-07 | 2022-02-23 | Lonza Ltd | Regulatable promoter |
| WO2014088563A1 (en) * | 2012-12-05 | 2014-06-12 | Exxonmobil Research And Engineering Company | Nannochloropsis kozak consensus sequence |
| PL2964765T3 (pl) | 2013-03-08 | 2019-10-31 | Keck Graduate Inst Of Applied Life Sciences | Promotory drożdżowe z pichia pastoris |
| DK3527667T3 (da) * | 2013-03-08 | 2021-02-22 | Biogrammatics Inc | Gærpromotorer til proteinekspression |
| PL2970994T3 (pl) | 2013-03-15 | 2020-03-31 | Lonza Ltd | Promotor konstytutywny |
| US9150870B2 (en) | 2013-03-15 | 2015-10-06 | Lonza Ltd. | Constitutive promoter |
| CN114365792A (zh) | 2014-11-11 | 2022-04-19 | 克莱拉食品公司 | 用于生成卵清蛋白的方法和组合物 |
| JP6833812B2 (ja) | 2015-08-05 | 2021-02-24 | ロンザ リミテッドＬｏｎｚａ Ｌｉｍｉｔｅｄ | プロモーター変異体 |
| CN108728477B (zh) * | 2017-04-24 | 2022-02-22 | 华东理工大学 | 一种高效的转座突变***及构建方法 |
| US11597845B2 (en) * | 2017-06-30 | 2023-03-07 | Dow Global Technologies Llc | Coating for aldehyde remediation and method of making |
| WO2019236451A1 (en) * | 2018-06-07 | 2019-12-12 | Basf Se | Promoter for yeast |
| US20220033832A1 (en) * | 2018-09-24 | 2022-02-03 | Orta Dogu Teknik Universitesi | Modified aox1 promoter variants |
| CN112739819A (zh) * | 2018-09-24 | 2021-04-30 | 中东科技大学 | 利用启动子工程化设计醇脱氢酶2(adh2)启动子变体 |
| CN110029120B (zh) * | 2019-03-19 | 2022-03-01 | 青岛蔚蓝生物集团有限公司 | 一种植酸酶高产菌株及其应用 |
| CN109735547B (zh) * | 2019-03-19 | 2022-05-31 | 青岛蔚蓝生物集团有限公司 | 一种提高毕赤酵母外源蛋白表达量的启动子及其应用 |
| CN110029122B (zh) * | 2019-03-19 | 2022-03-01 | 青岛蔚蓝生物集团有限公司 | 一种甘露聚糖酶高产菌株及其应用 |
| BR112021020727A2 (pt) * | 2019-04-17 | 2021-12-14 | Impossible Foods Inc | Materiais e métodos para produção de proteína |
| CN110106168B (zh) * | 2019-05-27 | 2021-07-13 | 西南大学 | 基于转录因子间的互作关系构建双激素响应启动子的方法 |
| JP2022539879A (ja) | 2019-07-11 | 2022-09-13 | クララ フーズ カンパニー | タンパク質組成物およびその消費可能な製品 |
| US10927360B1 (en) | 2019-08-07 | 2021-02-23 | Clara Foods Co. | Compositions comprising digestive enzymes |
| CN110724720A (zh) * | 2019-11-01 | 2020-01-24 | 山东仙普爱瑞科技股份有限公司 | 一种猪α-干扰素的发酵工艺 |
| JP2024506650A (ja) | 2021-02-12 | 2024-02-14 | ベーリンガー インゲルハイム エルツェーファウ ゲゼルシャフト ミット ベシュレンクテル ハフツング ウント コンパニー コマンディトゲゼルシャフト | 増加したタンパク質分泌のためのシグナルペプチド |
| CA3131181C (en) * | 2021-06-24 | 2023-03-07 | BioBoost Synbio Consulting Inc. | Method for methanol free culturing of methylotrophic yeast for the biosynthesis of added value products |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2063890C (en) * | 1991-03-27 | 2007-03-13 | Masami Miura | Mutant aox2 promoter, microorganism carrying same, method of preparation thereof and production of heterologous protein using such microorganism |
| CA2058820C (en) * | 1991-04-25 | 2003-07-15 | Kotikanyad Sreekrishna | Expression cassettes and vectors for the secretion of human serum albumin in pichia pastoris cells |
| JPH06209763A (ja) * | 1993-01-13 | 1994-08-02 | Green Cross Corp:The | 変異株 |
| AU6415594A (en) * | 1993-03-25 | 1994-10-11 | Biosource Genetics Corporation | (pichia pastoris) alcohol oxidase (zza1) and (zza2) |
| JP3290108B2 (ja) * | 1997-08-29 | 2002-06-10 | 麒麟麦酒株式会社 | プロモーター活性を増大させるｄｎａ配列 |
| CN1192103C (zh) * | 1998-01-27 | 2005-03-09 | 三井化学株式会社 | 生产植酸酶的方法 |
| AU3910000A (en) * | 1999-03-22 | 2000-10-09 | Zymogenetics Inc. | Improved methods for producing proteins in transformed (pichia) |
| CN1188524C (zh) * | 2001-02-28 | 2005-02-09 | 珠江医院 | 人SP－A1在pichia pastoris中的表达 |
| MXPA03009059A (es) * | 2001-04-05 | 2004-10-15 | Univ Nebraska | Secuencias de nucleotidos reguladoras de alcohol oxidasa 1 para expresion de genes heterologos en levadura. |
| EP1431387B1 (en) * | 2002-12-20 | 2008-02-27 | Roche Diagnostics GmbH | Heat-labile desoxyribonculease I variants |
-
2005
- 2005-02-23 AT AT0030405A patent/AT501955B1/de not_active IP Right Cessation
-
2006
- 2006-02-23 IN IN6694DEN2012 patent/IN2012DN06694A/en unknown
- 2006-02-23 SI SI200630919T patent/SI1851312T1/sl unknown
- 2006-02-23 WO PCT/AT2006/000079 patent/WO2006089329A2/en active Application Filing
- 2006-02-23 CA CA2598514A patent/CA2598514C/en active Active
- 2006-02-23 CN CN2006800058881A patent/CN101128581B/zh active Active
- 2006-02-23 PL PL06704721T patent/PL1851312T3/pl unknown
- 2006-02-23 NZ NZ560542A patent/NZ560542A/en unknown
- 2006-02-23 ES ES10155180T patent/ES2358086T3/es active Active
- 2006-02-23 KR KR1020077021821A patent/KR101352399B1/ko active IP Right Grant
- 2006-02-23 EP EP10155180A patent/EP2199389B1/en active Active
- 2006-02-23 EP EP06704721A patent/EP1851312B9/en active Active
- 2006-02-23 NZ NZ585821A patent/NZ585821A/en unknown
- 2006-02-23 SI SI200630962T patent/SI2199389T1/sl unknown
- 2006-02-23 CN CN2010101738787A patent/CN101935657B/zh active Active
- 2006-02-23 DE DE602006018113T patent/DE602006018113D1/de active Active
- 2006-02-23 DK DK06704721.7T patent/DK1851312T5/da active
- 2006-02-23 BR BRPI0606179-6A patent/BRPI0606179B1/pt active IP Right Grant
- 2006-02-23 KR KR1020127018581A patent/KR101358663B1/ko active IP Right Grant
- 2006-02-23 UA UAA200710436A patent/UA94399C2/ru unknown
- 2006-02-23 ES ES06704721T patent/ES2356127T3/es active Active
- 2006-02-23 JP JP2007556461A patent/JP5497986B2/ja active Active
- 2006-02-23 UA UAA201012563A patent/UA98604C2/ru unknown
- 2006-02-23 AT AT06704721T patent/ATE487788T1/de active
- 2006-02-23 AU AU2006216094A patent/AU2006216094B2/en active Active
- 2006-02-23 PT PT06704721T patent/PT1851312E/pt unknown
- 2006-02-23 AT AT10155180T patent/ATE496125T1/de active
- 2006-02-23 US US11/817,005 patent/US9012175B2/en active Active
- 2006-02-23 DE DE602006019789T patent/DE602006019789D1/de active Active
- 2006-02-23 PL PL10155180T patent/PL2199389T3/pl unknown
- 2006-02-23 DK DK10155180.2T patent/DK2199389T3/da active
- 2006-02-23 CA CA2785161A patent/CA2785161C/en active Active
-
2008
- 2008-08-08 HK HK11102457.9A patent/HK1148313A1/xx not_active IP Right Cessation
- 2008-08-08 HK HK08108778.3A patent/HK1113172A1/xx not_active IP Right Cessation
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2010
- 2010-04-14 US US12/759,763 patent/US9279129B2/en active Active
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2011
- 2011-01-18 AU AU2011200187A patent/AU2011200187B2/en active Active
- 2011-02-03 HR HR20110084T patent/HRP20110084T2/hr unknown
- 2011-04-08 HR HR20110254T patent/HRP20110254T1/hr unknown
- 2011-11-09 JP JP2011245688A patent/JP5552107B2/ja active Active
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