TW200808332A - Pharmaceutical agent containing birch extract as an effective ingredient and its use - Google Patents

Abstract

To provide a promoter for production of profillagrin and/or fillagrin derived from natural products producing natural humectant factors in skin itself and to provide an external preparation for skin sustainedly maintaining the skin moistness by stably formulating the promoter. The promoter for the production of the profillagrin and/or fillagrin comprises sap of Betula platyphylla Sukatchev var. japonica Hara as an active ingredient. The external preparation for skin comprises the promoter.

Description

200808332 (1) 九、發明說明 【發明所屬之技術領域】 本發明係關於白樺（Betula platyphylla Sukatchev var. japonica Hara)樹液（以下僅以白樺樹液表示）作爲 有效成分之種種藥效劑，又，亦關於白樺樹液之種種藥效 劑的使用。該利用領域例如爲化妝料、醫藥部外品、外用 醫藥品等皮膚外用劑的領域。 【先前技術】 過去如乳液、乳霜、化妝水、美容液、面膜、洗淨劑 、分散液、軟膏、氣溶膠、貼劑、擦劑等等皮膚外用劑中 ，以賦予彼等所定藥效爲目的而加入種種藥效劑。 例如作爲改善皮膚粗糙、皺紋等之保濕成分，已提出 甘油或丙二醇、糖類、胺基酸類等（fUgrance journal臨 時增刊 vol.9 ( 1988)、第 116-117 頁；flagrance journal 臨時增刊 νο1·9 ( 1 98 8 )、第 1 27- 1 28 頁；flagrance journal vol .23 ' No. 1 ( 1 995 )第 71-79 頁）。 * 對於藉由塗佈含有保濕成分之皮膚外用劑，雖已嘗試 維持肌膚的濕潤，但至今作爲保濕成分之效果僅爲暫時性 之保濕效果，無法於長時間下維持肌膚的保濕。欲維持長 期間之肌膚保濕，必須先瞭解該保濕機制，提供一種直接 對該機制作直接性或間接性作用之用劑。 作爲經長時間可維持肌膚保濕之其中一要因’可舉出 促進肌膚本身之天然保濕因子的原聚角微絲蛋白（ -5- 200808332200808332 (1) IX. Description of the invention [Technical field to which the invention pertains] The present invention relates to various medicinal agents of Betula platyphylla Sukatchev var. japonica Hara sap (hereinafter, only expressed by birch sap) as an active ingredient, and The use of various pharmacodynamic agents for birch sap. The field of use is, for example, in the field of skin external preparations such as cosmetics, pharmaceutical products, and external medicines. [Prior Art] In the past, such as lotions, creams, lotions, lotions, masks, detergents, dispersions, ointments, aerosols, patches, liniments, etc., to give them the efficacy of the skin. Various pharmacodynamic agents are added for the purpose. For example, glycerin or propylene glycol, saccharides, amino acids, etc. have been proposed as moisturizing ingredients for improving skin roughness, wrinkles, etc. (fUgrance journal temporary supplement vol. 9 (1988), pp. 116-117; flagrance journal temporary supplement νο1·9 ( 1 98 8 ), pp. 1 27- 1 28; flagrance journal vol .23 ' No. 1 (1 995) pp. 71-79). * Although it has been tried to maintain the moisturization of the skin by applying a skin external preparation containing a moisturizing ingredient, the effect as a moisturizing ingredient has only been a temporary moisturizing effect, and it is impossible to maintain the skin moisturizing for a long period of time. To maintain moisturization of the skin for a long period of time, it is necessary to first understand the moisturizing mechanism and provide a direct or indirect effect on the machine. As one of the main reasons for maintaining skin moisturization over a long period of time, it can be used to promote the natural moisturizing factor of the skin itself. (5-200808332

profilaggrin)及/或聚角微絲蛋白（filaggrin)之產生。 然而，對於促進該原聚角微絲蛋白（profilaggrin )及/或 聚角微絲蛋白（filaggrin)之產生的用劑至今爲未知。 又，作爲對於改善肌膚表皮之阻礙功能降低所引起的 肌膚粗糙等肌膚問題之用劑已被提出Ceramide (賽絡美） (flagrance journal 臨時增刊 vol. 15 ( 1996)第 117-127 頁）、保濕促進或阻礙功能之補助爲目的之用劑、膽固醇 衍生物（flagrance journal 臨時增刊 vol. 17 ( 2000)第 1 23 - 1 2 8頁）、作爲皮膚保護爲目的之用劑的膨潤石（ Smectite) ( flagrance journal 臨時增刊 vol. 17 ( 2000 )第 1 75- 1 84 頁）等。 上述賽絡美已知爲作爲構成細胞脂質之成分，作爲抑 制由皮膚之水分損失的角層阻礙功能上爲極重要角色。另 一方面，該角層阻礙功能中，包殼蛋白（involucrin)亦 擔任重要角色。如上述賽絡美，對於過去使用於角層阻礙 功能降低防止之用劑，補充細胞間脂質或肌膚表皮上形成 皮膜而達到阻礙功能之改善效果者爲多，藉由促進包殼蛋 白（involucrin )之產生而達成阻礙功能的改善效果者則 尙未知。 又，膽固醇亦爲角層細胞間脂質的一構成成分（佔角 層細胞間脂質之約25%的成分），已知可防止由表皮之水 分蒸散。因此，欲要維持肌膚的濕潤，必須提高膽固醇之 產生（flagrance journal 臨時增刊 Νο·17 ( 2000)第 20- 2 6頁）。然而，對於促進該膽固醇之產生的用劑尙未知。 -6 - 200808332 (3) 又，過去隨著年齡增加會使得細胞代謝功能降低，成 爲皮膚的新陳代謝速度之降低或皮膚粗糙的原因，又造成 膠原蛋白或彈力蛋白等真皮矩陣的減少或變性，與皺紋或 皮膚彈性降低等現象有關。且，因老化廢棄物無法順利排 出亦爲色素沈澱或皮膚黯淡的原因。以防止或改善這些現 象的進行，正探討達到細胞活化且促進增殖之用劑，即探 討細胞賦活劑。例如已提出作爲細胞賦活劑之海蘊萃取物 (特開2003 -2678 52號公報參照）、或由番紅花的雄蕊得 * 到之萃取物（特開2 0 0 5 - 0 4 1 8 1 2號公報參照）等。 如上所示之細胞賦活劑，作爲最近皮膚外用劑的添加 成分，由安全性等面來看，與化學物質相比來自天然物質 的成分有較佳的傾向。例如，嘗試將來自天然物之材料的 白樺類之萃取液或樹液使用於皮膚外用劑的成分上。例如 白樺萃取液具有保濕作用，組合該白樺萃取液與西印度標 桃（acerola)萃取物之皮膚老化防止用或肌膚粗糙改善用 之皮膚外用劑（特開2000-2 1 2027號參照）、或添加白樺 樹液之保濕作用優良的肌膚粗縫改善用化妝料（特開〇 9 _ • 2 9 1 0 1 3號參照）。 然而’白樺萃取液或白樺樹液具有上述原聚角微絲蛋 白（profilaggrin)或聚角微絲蛋白（filaggrin)產生促進 作用、包殼蛋白（involucrin )產生促進作用、膽固醇產 生促進作用或細胞賦活作用則完全爲未知。 【發明內容】 -7- 200808332 (4) 本發明有鑑於上述課題所成者本發明的第1課題爲提 供一種促進來自天然物質的原聚角微絲蛋白（pr0filaggrin )或聚角微絲蛋白（filaggrin)產生的新穎用劑； 第2課題爲提供一種促進來自天然物質的包殼蛋白（ involucrin)產生的新穎用劑； 第3課題爲提供一種促進來自天然物質的膽固醇產生 之新穎用劑；及 第4課題爲提供一種具有來自天然物之細胞賦活作用 " 的新穎用劑。 有鑑於上述本發明者欲解決上述課題，著重於來自天 然物之成分中的來自植物的成分，進行詳細硏究結果，作 爲具有上述作用的用劑，發現白樺樹液具有其效果。 即，本發明係關於白樺 （Betula platyphylla Sukatchev var. japonica Hara)樹液作爲有效成分之原聚 角微絲蛋白（pro filaggrin )、或聚角微絲蛋白（f i 1 a g g r i η )產生促進劑；包殼蛋白（involucrin )產生促進劑；膽 固醇產生促進劑；及細胞賦活劑。 ^ 又，本發明由另一觀點來看，提供可作爲白樺（Profilaggrin) and/or production of filaggrin. However, agents for promoting the production of profilaggrin and/or filaggrin have not been known until now. In addition, it has been proposed as a medicinal agent for skin problems such as skin roughness caused by a decrease in the barrier function of the skin epidermis. Ceramide (flagrance journal vol. 15 (1996) pp. 117-127), moisturizing A propellant for the purpose of promoting or impeding the subsidy of the function, a cholesterol derivative (flagrance journal, Temporary Supplement vol. 17 (2000), pp. 1 23 - 1 2 8 ), and a bentonite (Smectite) for the purpose of skin protection. (flagrance journal temporary supplement vol. 17 (2000) pp. 1 75- 1 84) and so on. The above-mentioned celestament is known as a component constituting cellular lipids, and plays a very important role as a function of inhibiting the cornea barrier caused by moisture loss of the skin. On the other hand, the involucrin also plays an important role in the barrier function of the corner layer. For example, in the case of the above-mentioned celestament, it is used in the past for the prevention of the prevention of the prevention of the protective layer of the cornea, and the effect of the formation of the membrane on the intercellular lipid or the epidermis of the skin to improve the barrier function, by promoting the involucrin. It is unknown that the effect of improving the barrier function is achieved. Further, cholesterol is also a constituent component of corneal intercellular lipids (a component which accounts for about 25% of intercellular lipids), and it is known to prevent evapotranspiration from water of the epidermis. Therefore, in order to maintain the moisturization of the skin, it is necessary to increase the production of cholesterol (flagrance journal Temporary Supplement Νο. 17 (2000), pp. 20-26). However, the agent for promoting the production of this cholesterol is unknown. -6 - 200808332 (3) In addition, in the past, with the increase of age, the metabolism of cells will be reduced, which will reduce the metabolic rate of the skin or the cause of rough skin, and cause the reduction or denaturation of the dermis matrix such as collagen or elastin. Wrinkles or skin elasticity are reduced. Moreover, the aging waste cannot be discharged smoothly, which is also the cause of pigmentation or dull skin. In order to prevent or improve the progress of these phenomena, an agent for achieving cell activation and promoting proliferation, that is, a cell activating agent, is being explored. For example, it has been proposed as a cell activating agent, and it is an extract obtained from the stamen of saffron (Special opening 2 0 0 5 - 0 4 1 8 1 2) No. bulletin reference). The cell activating agent as described above has a tendency to be a component derived from a natural substance as compared with a chemical substance, as an additive component of a recent skin external preparation. For example, an attempt is made to use an extract or sap of white birch from a material of a natural material for the composition of the external preparation for skin. For example, the white birch extract has a moisturizing effect, and the skin external preparation for skin aging prevention or skin roughness improvement of the white birch extract and the acerola extract (refer to JP-A No. 2000-2 1 2027), or Add a cosmetic material that is excellent in moisturizing effect of white birch sap (Special opening 9 _ • 2 9 1 0 1 3). However, 'Betula platyphylla or birch sap has the above-mentioned profilaggrin or filaggrin production promoting effect, involucrin production promoting effect, cholesterol production promoting effect or cell activating effect It is completely unknown. SUMMARY OF THE INVENTION The present invention has been made in view of the above problems, and a first object of the present invention is to provide a pro-aggregated microfilament protein (profiggrgrin) or a polyhedral fibroin (from a natural substance). a novel agent produced by filaggrin; a second object is to provide a novel agent for promoting the production of an involucrin from a natural substance; and a third object is to provide a novel agent for promoting the production of cholesterol from a natural substance; The fourth problem is to provide a novel agent having a cell activation effect from a natural product. In view of the above-mentioned problems, the inventors of the present invention have focused on the plant-derived components from the components of natural materials, and as a result of the detailed investigation, it has been found that the birch sap has an effect as an agent having the above-described effects. That is, the present invention relates to a profilaggrin or a fi fibrin (fi 1 aggri η ) production promoter of Betula platyphylla Sukatchev var. japonica Hara sap as an active ingredient; Protein (involucrin) production promoter; cholesterol production promoter; and cell activating agent. ^ Again, the present invention is provided by another point of view as a white birch (

Betula platyphylla Sukatchev v ar. j aponica H ar a )樹液的 原聚角微絲蛋白（profilaggrin )或聚角微絲蛋白（ filaggrin)產生促進劑使用；可作爲包殼蛋白（involucrin )產生促進劑使用；可作爲膽固醇產生促進劑使用；及可 作爲細胞賦活劑使用。 又，由本發明的另一觀點來看，本發提供一種藉由使 -8- 200808332 (5) 用白樺（Betula platyphylla Sukatchev var. japonica Hara )樹液而促進原聚角微絲蛋白（profilaggrin)或聚角微絲 蛋白（filaggrin)產生之方法；促進包殼蛋白（inv〇lucrin )產生之方法；促進膽固醇產生方法；及細胞賦活方法（ 但，任皆除去對於人類的治療方法）。 本發明爲提供一種來自天然物，且安全性良好之新穎 原聚角微絲蛋白（profilaggrin )或聚角微絲蛋白（ filaggrin)產生促進劑、包殼蛋白（inv〇iucrin)產生促進 ^ 提供、膽固醇產生促進劑、及細胞賦活劑。 又，本發明的含有原聚角微絲蛋白（profilaggrin)及 /或聚角微絲蛋白（filaggrin)產生促進劑之皮膚外用劑可 對肌膚本身促進天然保濕因子之產生，可持續維持肌膚的 濕潤。 又，本發明的含有包殼蛋白（involucrin)產生促進 劑之皮膚外用劑，可期待使肌膚表皮的阻礙功能正常化， 使肌膚表皮的水分保持得到改善效果，且可達到肌膚粗糙 ' 防止效果等。 • 又，本發明的含有膽固醇產生促進劑之皮膚外用劑， 可對於肌膚本身促進膽固醇的產生，其結果可防止水分蒸 散，持續維持肌膚的濕潤。 又，本發明的含有細胞賦活劑之皮膚外用劑’可達到 抑制或改善肌膚粗糙、皮膚黯淡等優良美膚效果。 以下對本發明作詳細説明。且，本說明書中所謂「〜 」爲前後所記載之數値表示含有下限値及上限値的意義。 -9- 200808332 (6) 本發明所使用的白樺（Betula platyphylla Sukatchev var· japonica Hara )爲樺木科植物，其別名亦稱爲白樺、 雁皮，分佈於岐阜縣以東的本州、北海道、千島列島、薩 哈林、朝鮮、中國、西伯利亞之落葉高木，本發明所使用 的白樺樹液爲，僅於早春至開花及開葉的！個月間，於樹 幹上穿孔所溢出之樹液。作爲該樹液的成分含有礦物質、 糖類、有機酸、胺基酸等。且，本發明中並無特別限定該 栽培方法或產地。 本發明所使用的白樺樹液之調製法並無特別限定，但 作爲本發明所使用的調製法一例可舉出由樹幹採取之樹液 經9 5 °C下1 0分鐘加熱滅菌後冷卻、過濾者再放置於5 °C 中3天，過濾沈澱物後所得者等。又，可利用白樺樹液之 販賣品，作爲販賣品可舉出森之—（莎布有限公司製）等 〇 對於聚角微絲蛋白（filaggrin )及原聚角微絲蛋白（ profilaggrin )作說明。聚角微絲蛋白（filaggrin )爲分子 量30萬以上之前驅蛋白，原聚角微絲蛋白（pr0filaggrin )爲分解至胺基酸等天然保濕因子的過程中之中間代謝物 。該原聚角微絲蛋白（Profilaggrin )爲，表皮顆粒細胞隨 著角層細胞的移動而受到蛋白分解酵素之分解，最後成爲 可維持皮膚表皮濕潤的天然保濕因子（皮膚科Mook、 No. 1 5 ( 1 9 8 9 )、第49-5 5頁）。因此，欲維持肌膚的濕 潤必須含有原聚角微絲蛋白（profilaggrin )及/或聚角微 絲蛋白（filaggrin )。本發明爲使用白樺樹液而促進原聚 -10- 200808332 (7) 角微絲蛋白（profilaggrin )及/或聚角微絲蛋白（ filaggrin )的產生。 包殼蛋白（involucrin )爲隨著表皮角化細胞之分化 藉由麩醯基轉移（Transglutaminase )交聯，於細胞周圍 形成不溶之膜Cornified Envelope (CE)，其爲CE前驅 蛋白。該包殼蛋白（involucrin )之細胞膜藉由與細胞間 脂質之賽絡美的一部份結合，形成角層阻礙功能。即，角 層阻礙功能的形成中，不僅細胞間脂質之賽絡美，與其結 合之膜蛋白的包殼蛋白（involucrin)之存在亦非常重要 。本發明爲使用白樺樹液而可促進包殼蛋白（involucrin )之產生。 膽固醇爲角層細胞間脂質的一構成成分（佔據角層細 胞間脂質的約25%之成分），其顯示防止由表皮蒸發水分 之功能。又，已知經老化之皮膚爲產生膽固醇之功能已劣 化。欲保持濕潤的皮膚，必須促進含於細胞間脂質之膽固 醇的產生。本發明因使用白樺樹液可促進含於細胞間脂質 之膽固醇的產生。 _ 又，因年齡的增加等所引起的細胞代謝功能之下降， 已知其成爲皮膚新陳代謝速度降低或肌膚粗糙的原因，或 引起膠原蛋白或彈力蛋白等真皮矩陣之減少或產生改性， 與皺紋或皮膚彈性低下等現象有關。且，因廢棄物無法順 利排出而成爲色素沈澱或皮膚黯淡之原因，欲防止或改善 這些現象的進行，故必須求得細胞之活化。本發明爲因使 用白樺樹液可活化細胞。 -11 - 200808332 (8) 本發明的原聚角微絲蛋白（p r o f i 1 a g g r i η )或聚角微絲 蛋白（filaggrin)產生促進劑、包殼蛋白（involucrin)產 生促進劑、膽固醇產生促進劑、或細胞賦活劑之用途並無 特別限定，將來自天然物之白樺樹液作爲有效成分使用時 ’其安全性良好，由相關觀點得知可作爲皮膚外用劑的藥 效成分使用時爲佳。皮膚外用劑之本發明的藥效劑之含有 量並無特別限定，但其有效成分的白樺樹液含有量爲 0.000 1〜50質量。/〇(以下僅以「％」表示）爲佳，較佳爲 0.001〜30%。僅爲該範圍內，可安定地含有該樹液，且具 有較高原聚角微絲蛋白（profilaggrin)或聚角微絲蛋白（ filaggrin)產生促進作用、包殼蛋白（involucrin)產生促 進作用、膽固醇產生促進作用、或細胞賦活作用，藉此結 果可發揮保濕效果及其持續效果。 又，將本發明中作爲種種藥效劑所使用的白樺樹液添 加於皮膚外用劑中時，可並用於皮膚外用劑常用的各種其 他藥效劑，例如一種或二種以上選自抗氧化劑、消炎劑、 細胞賦活劑、紫外線防止劑、保濕劑、血循環促進劑等藥 ' 效劑，可更提更本發明之效果。 作爲這些藥效劑之具體例子，各可舉出下述者。其中 「衍生物」爲含有可能形成之酯或鹽。又，有時一種物質 中具有複數種藥效，故複數種的藥效劑以一名稱記載。（ 且，植物的情況爲，別名的漢藥名等記載於括弧內）。 作爲抗氧化劑可舉出維他命E及其衍生物、維他命C 及其衍生物、類胡蘿蔔素、二丁基羥基甲苯、丁羥基苯甲 -12- (9) (9)200808332 醚、芸香素及其衍生物、麩胱甘肽及其衍生物、超氧化物 歧化酵素、甘露糖醇、雞血藤萃取物、尼泊爾老鸛草萃取 物、山楂子萃取物、芍藥萃取物、銀杏萃取物、黃金花萃 取物、玫瑰花（山楂花、濱梨）萃取物、P. tormentilla萃 取物、葡萄萃取物、矢車實（矢車）萃取物、草莓虎耳草 萃取物、迷迭香 （R.officinalis)萃取物、茶萃取物（烏 龍茶、紅茶、綠等）等。 作爲可舉出消炎劑、甘草酸及其衍生物、甘草次酸及 其衍生物、硫及其衍生物、蘆薈萃取物、明日葉萃取物、 蓴麻萃取物、茵藤嵩（Artemisia capillaris)萃取物、戀 金萃取物、黃菠蘿（黄柏）萃取物、小連翹萃取物、洋甘 菊萃取物、康復力（鰭玻璃草）萃取物、忍冬（金銀花） 萃取物、和蘭芥子萃取物、鼠尾草（sage )萃取物、吾亦 紅（榆）萃取物、紫蘇萃取物、白樺萃取物、接骨木萃取 物、香蒲（蒲黄）萃取物、無患子萃取物、尤加利萃取物 、宿根草萃取物、蓮華草萃取物、赤竹萃取物、氧化鋅等 〇 作爲細胞賦活劑可舉出維他命A及其衍生物、蘆筍萃 取物、大豆萃取物、棗（大棗）萃取物、蒜頭萃取物、人 參萃取物、殻斗萃取物、蜂王漿、酵母萃取物、微生物發 酵代謝產物、靈芝萃取物等。 作爲紫外線防止劑可舉出對甲氧基桂皮酸-2-乙基己酯 、4-第三丁基-4’·甲氧基二苯甲醯基甲烷、羥苯甲酮及其 衍生物、氧化鈦、微粒子氧化鈦、氧化鋅及微粒子氧化鋅 -13- 200808332 (10) 等。氧化鈦、氧化鋅等無機粉體使用微粒子者時可發揮更 高效果。 作爲保濕劑可舉出甘油、1，3-丁二醇、蛋白質或此等 之衍生物或水解物以及這些鹽（膠原蛋白、彈力蛋白等） 、透明質酸及其鹽、胺基酸及此等之衍生物、糖類、D-泛 酸醇及其衍生物、糖脂質、磷脂質、賽絡美、尿素、黏蛋 白、杏仁萃取物、酪梨萃取物、屬葵萃取物、溫泉水、蘆 薈萃取物、大花葵萃取物、刺芒柄花萃取物、野燕麥萃 取物、光甘草定、光果甘草素、liqnilitin、isoliquilitin 及含有此等之甘草萃取物、溫椁種子萃取物、苦參萃取物 、梔子花萃取物、葡萄柚萃取物、和蘭芥子萃取物、龍膽 萃取物、龍膽老鸛草萃取物、牛蒡萃取物、小麥萃取物、 辣椒素萃取物、仙人掌萃取物、石鹼草萃取物、山楂子萃 取物、地黃萃取物、下野草萃取物、生薑萃取物、大花葵 花（薄紅立葵）萃取物、桑（桑白皮）萃取物、銀斑百里 香（百里香）萃取物、冬蟲夏草萃取物、魚腥草萃取物、 薄荷萃取物、薏仁萃取物、金縷梅萃取物、薔薇萃取物、 ' 檜萃取物、款冬花萃取物、葡萄萃取物、蜜棗萃取物、絲 瓜萃取物、菩提樹萃取物、蛇麻草萃取物、松萃取物、梓 萃取物、七葉樹萃取物、香蜂草萃取物、矢車草萃取物、 百合萃取物、萊姆萃取物、薰衣草萃取物、蘋果萃取物、 米及米糠萃取物、黑醋栗萃取物、拳參萃取物、野薔薇萃 取物、刺五加萃取物、海藻萃取物等。 且’作爲藉由皮膚表面封閉之保濕劑可舉出荷荷芭油 -14- 200808332 (11) 、澳洲堅果油、橄欖油、杏仁油、杏仁油、葵花油、向 日葵油、酪梨油、白芒花好油 （Meadowfoam)、椿油、 杏仁油、荏胡麻油、芝麻油、琉璃苣油、可可脂、乳油木 果脂等。 作爲血液循環促進劑可舉出柚子萃取物、山金車萃 取物、辣椒酊、菖蒲萃取物、r -谷維素等。 這些抗氧化劑、消炎劑、細胞賦活劑、紫外線防止劑 、保濕劑、血液循環促進劑的各成分可組合一種或二種以 上使用。 又，含有本發明藥效劑之皮膚外用劑中除上述成分以 外亦可添加一般使用於化妝料或醫藥部外品、外用醫藥品 等之各種成分，即水、醇類、多元醇、油劑、界面活性劑 、增黏劑、粉體、螯合劑、殺菌劑、酵素、pH調整劑、 來自動植物·微生物之萃取物、香料等，以不損害本發明 的效果之範圍內可適宜添加。 作爲含有本發明的藥效劑之皮膚外用劑的添加形態例 子，並無特別限定，例如可使用選自乳液、乳霜、化妝水 ' 、美容液、面膜、洗淨劑、彩妝化妝料、分散液、軟膏、 液劑、氣溶膠、貼付劑等之任意形態的化妝料、或外用醫 藥品等。 【實施方式】 〔實施例〕 其次舉出製造例、試驗例、實施例對本發明作更具體 -15- 200808332 (12) 説明，但本發明未受到這些之任何限定。 試驗例1 :原聚角微絲蛋白（profilaggrin )及/或聚角微絲 蛋白（filaggrin)產生量測定 作爲白樺樹液（商品名「森之雫」、莎布有限公司製 )的對照組，使用已知具有對於人類表皮細胞可提高分化 促進作用之鈣溶液（氯化鈣溶液；以下僅稱爲「鈣溶液」 ))，又，將僅以培養基生長者作爲對照組，對於賦予原 聚角微絲蛋白（profilaggrin )及/或聚角微絲蛋白（ filaggrin)產生量之作用作調查。正常人類新生而表皮細 胞經前培養後，欲防止培養基的浸透壓降低，對於培養基 量將白樺樹液 30v/v°/。、20v/v°/。、lOv/v%、lv/v%等量藉由 冷凍乾燥濃縮至1 /1 0倍後添加。又，對於對照組添加至 鈣溶液含於原先培養基中的鈣離子濃度至0.03mM/mL而 使總量爲1.5mM/mL，各培養基條件下進行一星期培養後 ，取出培養基，以PBS (-)洗淨一次後，回收表皮細胞 。其此於回收的細胞中添加ρΗ7·5的1 OmMTris-NaCl緩衝 液（含有8M尿素、1 v/v%Triton X -100、蛋白酶抑製劑雞 尾酒（protease inhibitor cocktail)(調整 EDTA、PMSF 、N M F至最終濃度爲1 m Μ ))後，以超音波於4 °C下進行 1 〇分鐘處理使細胞破碎，經由離心分離取出細胞殘渣後進 行蛋白質定量，欲使各樣品之蛋白質量爲一定，於4〜 12%的聚丙烯醯胺凝膠中處理樣品，進行SDS-PAGE。電 泳終了後於凝膠上將蛋白質轉錄於硝纖維素膜，藉由進行 -16- 200808332 (13) 西方墨點法檢測出原聚角微絲蛋白（profilaggrin )及/或 聚角微絲蛋白（filaggrin) 。1次抗體爲使用抗人類聚角 微絲蛋白（filaggrin)來自老鼠之單株抗體（Biomedical Technologies公司製），2次抗體爲使用抗老鼠過氧化酶 標識IgG ( Amersham公司製）藉由化學發光法進行檢測 。經檢測之一段以螢光測定儀讀取，測定面積作爲生成量 ’對於對照組的生成量比如表1所示。 [表1] 試料名 原聚角微絲蛋白及/或 聚角微絲蛋白產生能 (對照組爲100) 白樺樹液 30v/v%等量添加 1099 白樺樹液 20v/v%等量添加 12 16 白樺樹液 10Wv%等量添加 565 白樺樹液 lv/v%等量添加 216 鈣 388 由表1得知白樺樹液及鈣中具有同時促進原聚角微絲 蛋白（profilaggrin)及/或聚角微絲蛋白（filaggriii)之 作用。對於錦白樺樹液爲1 v/v%濃度時顯示較爲差，此以 上之試驗濃度中則顯示較高之原聚角微絲蛋白（ profilaggrin )及/或聚角微絲蛋白（fiiaggriri )產生促進 作用。因此’白樺樹液與已知可促進原聚角微絲蛋白（ profilaggrin)及/或聚角微絲蛋白（filaggrin)產生之金丐 具有同等或較高原聚角微絲蛋白（profilaggrin)及/或聚 -17- 200808332 (14) 角微絲蛋白（filaggrin )產生促進作用。 試驗例2 :包殻蛋白（inv〇lucrin)產生量測定 作爲白樺樹液之對照組，使用已知對人類表皮細胞具 有較高分化促進作用之鈣溶液（氯化鈣溶液；以下僅記爲 「鈣溶液」），又將僅於培養基生長者作爲對照組，對於 賦予包殼蛋白（involucrin)產生量之作用做調查。正常 人類新生兒表皮細胞經前培養後，欲防止培養基的浸透壓 降低，添加對培養基量爲 30v/v%、20v/v°/〇、10v/v%、 lWv%等量之經冷凍乾燥後濃縮至1/10倍的白樺樹液。又 ，對照組中添加鈣溶液至加上原先培養基中所含之鈣離子 濃度0.0 3mM/mL使總量爲1 .5mM/mL，各培養基條件下進 行一星期培養後，取出培養基以PBS (-)進行1次洗淨 後回收表皮細胞。其次於回收細胞中添加 PH7.5的 1 OmMTris-NaCl 緩衝液（含有 8 Μ 尿素、1 v/v%Triton X-1〇〇、蛋白酶抑製劑雞尾酒（protease inhibitor cocktail) (調整各EDTA、PMSF、NMF最終濃度爲ImM))後， " 以超音波於4°C下進行1 〇分鐘處理使細胞破碎，經離心分 離取出細胞殘渣後進行蛋白質定量，欲使各樣品的蛋白質 量爲一定，於4〜12%的聚丙烯醯胺凝膠進行樣品處理， 進行SDS-PAGE。電泳終了後，將凝膠上的蛋白質轉錄於 硝纖維素膜上，藉由進行西方墨點法檢測包殼蛋白（ involucrin ) 。1次抗體爲使用抗人類包殼蛋白（ involucrin)來自老鼠的單株抗體（abeam公司製），2次 -18- (15) (15)200808332 抗體爲使用抗老鼠過氧化酶標識IgG ( Amersham公司製 )藉由化學發光法進行檢測。經檢測的一段由螢光測定儀 讀取，測定面積作爲生成量’對於對照組的生成量比如表 2所示。 [轰2] 試料名 包殻蛋白產生能 (對照組爲100) 白樺樹液 3〇v/v%等量添加 1 57 白樺樹液 2〇v/v°/〇等量添加 154 白樺樹液 1 0 v / v °/〇等量添加 146 白樺樹液 1 v/v%等量添加 127 鈣 128 由表2得知白樺樹液及鈣皆具有促進包殼蛋白（ involucrin)產生之作用，對於鈣而言，白樺樹液具有較 高包殼蛋白（involucrin )產生促進作用。因此，可確認 白樺樹液必過去公知可促進包殼蛋白（involucrin)產生 之鈣具有更優良的包殻蛋白（involucrin )產生促進作用 試驗例3 :膽固醇產生率的測定 作爲白樺樹液的對照組，使用已知對於人類表皮細胞 可提高分化促進作用的鈣溶液（氯化鈣；以下僅記爲「鈣 溶液」），又僅以培養基下生長者作爲對照組，對於賦予 膽固醇產生之作用進行調查。將正常人類新生兒表皮細胞 -19- 200808332 (16) 進行前培養後，欲防止培養基的浸透壓降低，添加對於培 養基量爲 30v/v%、20v/v%、10v/v%、lv/v%各等量經冷凍 乾燥濃縮爲1 /1 0倍之白樺液。又，於對照組中添加鈣溶 液至加上原先培養基中所含之鈣離子濃度0.03mM/mL使 其總量爲1.5mM/mL，各培養基條件下進行一星期培養後 ，取出培養基再以PB S (-)進行1次洗淨後回收表皮細 胞。於該人類表皮細胞中添加水、氯仿及曱醇各2.5mL並 混合後，分離氯仿層並乾燥固體化。經萃取的脂質以 HPTLC 盤〔矽膠 G60 ( 20x10cm) Art.5 64 1 ； Merck 公司 製〕，經混合溶媒（氯仿：甲醇：乙酸=190 : 9 : 1 )進 行2次展開。噴霧40 %硫酸後以180度進行15分鐘加熱 後使其發色，將TLC上的膽固醇以螢光測定儀進行比色定 量。僅於培養基下生長的控制組作爲1 · 〇，算出膽固醇產 生促進率，其結果如表3所示。 [表1] 試料名 膽固醇產生能 (對照組爲100) 白樺樹液 3 0 v / v %等量添加 125 白樺樹液 2〇v/v%等量添加 123 白樺樹液 1 0 v / v %等量添加 117 白樺樹液 1 v/v%等量添加 108 鈣 105 由表3得知白樺樹液及鈣具有可促進膽固醇產生之作 用，但對於鈣而言白樺樹液’於所有試驗濃度中顯示較高 -20- 200808332 (17) 膽固醇產生促進作用。因此，白樺樹液比過去已知可促進 膽固醇產生之鈣具有更高膽固醇產生促進作用。 試驗例4 :對於表皮角化細胞之角化促進作用 將正常人類新生兒皮膚表皮角化細胞（KURABO公司 製）使用表皮角化細胞增殖用培養基（KURABO公司製） ，於直徑24多重格子盤上以6000個/格播種，於5%vol 二氧化碳環境下，於37t下進行24小時培養。培養後添 加白樺樹液至最終濃度1 〇%的例子（實施例1 )、作爲比 較例爲無添加的例子（比較例1 )及作爲陽性對象之添加 氯化鈣至最終濃度爲1 .5mM的例子（比較例2 )經3天培 養。培養後，對各試料的細胞進行攝影，調查其形態。攝 影的照片如圖1〜3所示。又，攝影後以MTT (3- (4,5-Dimethyl-2-thiazolyl) -2，5-diphenyl-2H-tetrazorium bromide) assay算出活細胞數。其結果如圖4所示。 如圖1〜3所示，由各細胞形態，添加白樺樹液之例 子（實施例1 :圖1 )及添加氯化鈣的例子（比較例2 :圖 ' 3 )與無添加這些成分之無添加比較例（比較例1 :圖2) 比較，發現細胞的肥大化與重層化，表示角質化被促進。 細胞的角化爲，皮膚新陳代謝速度越快，即代謝功能越高 越被促進，故角化越被促進，更顯示越高細胞賦活作用。 圖4表示各試料的活細胞數的圖。會呼吸的一般細胞 會因角化而停止呼吸，角質細胞若不剝離，培養細胞之氧 消耗量變少。因此，藉由定量氧消耗量可算出活細胞數。 -21 - 200808332 (18) 活細胞數越少的試料表示角化更被促進 胞賦活作用。 由圖4的結果得知，添加各白樺樹 氯化錦的g式料（貫施例1及比較例2 ) 比較例1 )相比，皆顯示活細胞數的更 的角化促進作用。 試驗例5 :細胞增殖促進作用 使用MTT assay法測定來自人類新 (NB1RGB)之細胞賦活率。MTT爲進 在於粒線體内之酵素（N A D Η )反應而 生成於5 70nm具有吸收波的不溶性甲臘 甲臘量與NADH量具有相關性，故可溶 的甲臘，經測定吸光度可測定間接性細 細胞數。 由24格微盤取出適量培養基，種 之線維芽細胞，於3 7 t下二氧化碳濃g 方面，取代一般使用的純水以白樺樹箱 (日水公司製），以一般MEM培養基 二天添加稀釋成白樺樹液含量爲7 5 °/〇 ( (實施例3 )之培養基。使用於一般培 爲對照組（比較例3)。培養第4天交 加試料。第二天欲使MTT ( SIGMA公1 將所製作的溶液1 〇 〇 # L添加於各格。； 。即，顯示較高細 液及公知角化劑之 與無添加的試料（ 減少，具有同程度 生兒的線維芽細胞 入活細胞中，與存 進行還原性開裂， 。已知這些生成的 化藉由本法所生成 胞能量代謝活性或 入來自人類新生兒 〔5 %中靜置。另一 【製造出依格MEM 做成稀釋系列。第 實施例2 )、1 0 0 % 養基進行培養者作 換培養基，再次添 3製）成爲5mg/mL 經4小時培養後， -22- 200808332 (19) 除去培養基，溶解使用異丙醇所生成的甲臘。使用微分 析儀，以6 3 0 n m作爲參考値，測定5 7 0 n m下的吸光度’ 算出對於對照之比率。其結果如圖5。 由圖5所示結果得知，添加白樺樹液的試料以濃度依 賴性下促進細胞增殖。由上述可確認白樺樹液中具有促進 細胞增殖之細胞賦活效果。 以下舉例出含有本發明的種種藥效劑之各種形態的皮 膚外用劑。且，實施例1 -1〜1 -9爲含有原聚角微絲蛋白（ ^ profilaggrin)或聚角微絲蛋白（filaggrin)產生促進劑之 皮膚外用劑的例子，實施例2-1〜2-9爲含有本發明的包殼 蛋白（involucrin )產生促進劑之皮膚外用劑的例子，實 施例3 -1〜3 -9爲含有本發明的膽固醇產生促進劑之皮膚外 用劑的例子，實施例4-1〜4-9爲含有本發明的細胞賦活劑 之皮膚外用劑的例子。 (實施例1 _ 1 :化妝水） (成分） （％ ) (1 )甘油 3.0 (2 ) 1，3-丁二醇 2.〇 (3 )丙二醇 _ 4.〇 (4 )油醇 〇 . ! (5 )單月桂酸聚環氧乙烷（20E.O·)山梨醇酐 15 (6)聚環氧乙烷（20E.O.)月桂醚 〇.5 (7 )三十碳院 0.5 -23- 200808332 (20) (8 )乙醇 15.0 (9 )乙酸dl- α -生育醇* 1 0.5 (1 〇 )香料 適量 (1 1 )甘草酸二鉀* 2 0.5 (12 )迷迭香萃取物* 3 0.5 (13 ) dl-吡咯烷酮羧酸鈉液* 4 0.2 (1 4 )刺槐豆膠 1.0 (15 )薏仁萃取物* 5 0.5 (16)白樺樹液*6 20.0 (1 7 )純水 剩餘量 * 1 Eisai公司製 *2 九善製藥公司製 *3 九善製藥公司製 * 4 味之素公司製 *5 九善製藥公司製 *6 莎布公司製（森之爭） (製法） A·成分（1 )〜（10 )經混合溶解。 B ·成分（1 1 )〜（1 7 )經混合溶解。 C . A中加入B後均勻混合得到化妝水。 (實施例1-2 :乳霜） (成分） （％ ) -24- 200808332 (21) (1 )三十碳烷 20.0 (2 )蜜蠟 5.0 (3 )荷荷芭油 5.0 (4 )單硬脂酸甘油酯 2.0 (5 )單硬脂酸聚環氧乙烷（20E.O·)山梨醇酐 2.0 (6 )單硬脂酸山梨醇酐 2.0 (7 )植物醇* 1 0.5 (8)堪地里鱲（Candelilla) 1.0 (9 )甘油 5.0 (1 〇 )對氧基安息香酸甲酯 0.1 (1 1 )百合萃取物* 2 1.0 (1 2 )人參萃取物* 3 1.0 (1 3 )銀杏萃取物* 4 1.0 (1 4 )大棗萃取物* 5 1.0 (1 5 )苦參萃取物* 6 1.0 (16)水解米萃取物*7 1.0 (1 7 )甘草萃取物* 8 1.0 (1 8 )當歸萃取物* 9 1.0 (19)白樺樹液*10 5.0 (2 0 )純水 剩餘量 (2 1 )香料 適量 氺1 Eisai公司製 * 2 九善製藥公司製 *3 九善製藥公司製 -25- (22) (22)200808332 * 4 常磐植物化學硏究所公司製 *5 九善製藥公司製 *6 九善製藥公司製 氺7 TECHNO-BULL公司製 *8 九善製藥公司製 *9 日本粉末藥品公司製 *10 莎布公司製（森之學） (製法） A.成分（1 )〜（10 )加熱混合後保持70°C。 B·成分（11)〜（20)加熱混合後保持70°C。 C.A中加入B後進行乳化。 D·冷卻後，C中加入成分（2 1 )而得到乳霜。 實施例1 -1及實施例1 -2皆使用於皮膚時，藉由原聚 角微絲蛋白（pro fi lag grin )及/或聚角微絲蛋白（ filaggrin )產生促進作用可對於肌膚本身產生天然保濕因 子，故其爲可持續性地維持肌膚的濕潤性的化妝水與乳霜 (實施例1-3 :潔面乳霜） (成分） （％ ) (1 )石蠟 10.0 (2 )蜜蠟 3.0 -26- 200808332 (23) (3 )凡士林 1 5 · 0 (4 )流動石蠟 41.0 (5)失水山梨醇倍半油酸酯 4.2 (6 )單油酸聚環氧乙烷（20E.O·)山梨醇酐 0.8 (7 )氫化大豆磷脂質 〇·5 (8)白芒花好油 （Meadowfoam) *1 1.0 (9 )對氧基安息香酸甲酯 0.1 (1 〇 )溫桴種子萃取物* 2 1.0 (1 1 )石鹼草萃取物* 3 1.0 (12) 大花葵花萃取物*4 1.0 (13) 白樺樹液*5 5.0 (1 4 )純水 剩餘量 (1 5 )香料 適量 * 1 日本界面活性劑公司製 *2 香榮興業公司製 *3 九善製藥公司製 * 4 九善製藥公司製 *5 莎布公司製（森之-) (製法） A·成分（1 )〜（9 )加熱混合後保持7(TC。 B. 成分（10)〜（14)加熱混合後保持70°C。 C. A中加入B後混合，均勻地進行乳化。 D. 冷卻後，C中加入成分（1 5 )後得到潔面乳霜。 -27- 200808332 (24) 實施例1 -3使用於皮膚時，可藉由原聚角微絲蛋白（ profilaggrin)及/或聚角微絲蛋白（filaggrin)產生促進 作用，而使肌膚本身產生天然保濕因子，故其爲可持續維 持肌膚滑潤的潔面乳霜。 (實施例1-4 :乳液） (成分） （％ ) (1 )單硬脂酸山梨醇酐 0.3 (2 )單油酸聚環氧乙烷（20E.O.)山梨醇酐 〇·1 (3 )親油型單硬脂酸甘油酯 0.2 (4 )硬脂酸 0.5 (5 )十六烷醇 0.5 (6)三十碳烷 3.0 (7 )流動石鱲 4.0 (8 )植物性三十碳烷* 1 0.2 (9) 三-2·乙基己酸甘油酯 2.0 (10) 二甲基聚矽氧烷 1.0 (1 1 )氫化大豆磷脂質 〇·1 (12)煙酸dl-α-生育醇*2 0.05 (1 3 )對氧基安息香酸乙酯 0.1 (14)羧基乙烯聚合物 0.1 (1 5 )氫氧化鈉 0.05 (1 6 )甘油 5.0 -28- 200808332 (25) (17) 1，3-丁二醇 7·0 (1 8 )乙醇 5.0 (1 9 )白樺樹液* 3 10.0 (20) 芍藥萃取物*4 2.0 (21) 靈芝萃取物*5 2.0 (2 2 )透明質酸鈉1 %水溶液* 6 5.0 (23)乙醯基麩胺酸*7 0.3 (24 )純水 剩餘量 (25 )多孔質二氧化矽 3.0 (26 )香料 適量 *1 日光化學公司製 氺2 Eisai公司製 *3 莎布公司製（森之雫） *4 九善製藥公司製 * 5 九善製藥公司製 *6 日光化學公司製 * 7 協和發酵公司製 (製法） A·成分（14)〜（23)加熱混合後保持70°C。 B·成分（1 )〜（13 )加熱混合後保持70°C。 C.A中加入B後混合，均勻地進行乳化。 D·冷卻後，C中加入成分（24 ) 、 （ 25 ) 、 （ 26 )後得到 乳液。 -29- 200808332 (26) (實施例1-5 :乳液） (成分） （％ ) (1 )單硬脂酸山梨醇酐 0.3 (2 )單油酸聚環氧乙烷（20E.O.)山梨醇酐 0.1 (3 )親油型單硬脂酸甘油酯 0.2 (4 )硬脂酸 0.5 (5) 十六院醇 0.5 (6) 三十碳垸 3.0 (7 )流動石蠟 4.0 (8 )三-2-乙基己酸甘油酯 2.0 (9 )二甲基聚矽氧烷 1.0 (1 〇 )氫化大豆磷脂質 0.1 (1 1 )芸香苷* 1 0.2 (12 )對氧基安息香酸甲酯 0.1 (13)羧基乙烯聚合物 0.1 (1 4 )氫氧化鈉 0.05 (15 )甘油 5.0 (16 ) 1, 3-丁二醇 7.0 (17)白樺樹液*2 3.0 (1 8 )純水 剩餘量 (1 9 )乙醇 5.0 (20)酵母萃取物*3 0.5 (2 1 )明日葉萃取物* 4 0.5 -30- (27) (27)200808332 (2 2 )蛇麻草萃取物* 5 〇5 (23 )桃子萃取物* 6 〇 5 (2 4 )多孔質二氧化矽 3.〇 (25)香料 適量 * 1 Sigma公司製 * 2 莎布公司製（森之—） * 3 BIO-DELL 公司製 *4 九善製藥公司製 *5 九善製藥公司製 *6 九善製藥公司製 (製法） A·成分（13 )〜（23 )加熱混合後保持70。(：。 B·成分（1 )〜（12 )加熱混合後保持70。(：。 C.A中加入B後混合，均勻地進行乳化。 D ·冷卻後，C中加入成分（24 ) 、（ 2 5 )得到乳液。 實施例1-4及實施例1-5皆使用於皮膚時，可藉由原 聚角微絲蛋白（p r 〇 f i 1 a g g r i η )及/或聚角微絲蛋白（ filaggrin )產生促進作用使肌膚本身產生天然保濕因子， 故其爲可持續維持肌膚滑潤的乳液。 (實施例1-6 :面膜） (成分） （％ ) -31 - 200808332 (28) 15.0 0.5 0.5 0.2 5.0 5.0 剩餘量 1.0 (1 )聚乙烯醇 (2 )無水矽酸 (3 )聚乙二醇1500 (4) 白芒花好油 （Meadowfoam) *1 (5) 聚環氧丙烷（10Ρ·Ο·)甲基葡萄苷 (6 )甘油 (7 )純水 (8 )茶萃取物* 2 (9)精油玫瑰（RosarugosaThunb)萃取物 *3 1.0 (1 0 )無水咖啡因* 4 1.0 (11) 白樺樹液*5 5.0 (12) 磷酸-L-抗壞血酸鎂*6 3.0 (1 3 )純水 5.0 (1 4 )乙醇 18.0 (1 5 )對氧基安息香酸乙酯 0.1 (1 6 )香料 適量 * 1 日本界面活性劑公司製 *2 九善製藥公司製 *3 九善製藥公司製 * 4 純正藥品工業公司製 * 5 莎布公司製（森之—） 氺6 日光化學公司製 (製法） -32 200808332 (29) Α·成分（1 )〜（7 )混合後於70°C中加熱並溶解。 B ·成分（8 )〜（1 3 )經混合溶解。 C ·成分（1 4 )〜（1 6 )經混合溶解。 D . A中加入B混合後，冷卻下加入C混合得到面膜。 實施例1 -6使用於皮膚時，可藉由原聚角微絲蛋白（ ^ Profilaggrin )及/或聚角微絲蛋白（filaggrin )產生促進 作用使得肌膚本身產生天然保濕因子，故其爲可持續維持 肌膚滑潤的面膜。 (實施例1-7 :乳霜粉底） (成分） （％ ) (I) 失水山梨醇倍半油酸酯 3.0 (2 )自己乳化型單硬脂酸甘油酯 2.0 (3 )硬脂酸鋁 0.5 (4) 純地鱲 5.0 (5) 三十碳院 10.0 (6 )流動石蠟 15.0 (7 )凡士林 5.0 (8 )氧化鈦 8.0 (9 )滑石 4.0 (1 〇 )著色顔料 適量 (II) 對氧基安息香酸甲酯 0.05 (1 2 )丙二醇 5.0 -33 200808332 (30) (1 3 )金銀花萃取物* 1 1.0 (14)白樺萃取物*2 1.0 (1 5 )七葉樹萃取物* 3 1.0 (1 6 )白樺樹液* 4 10.0 (1 7 )純水 剩餘量 (1 8 )香料 適量 *1 長岡香料公司製 * 2 九善製藥公司製 *3 香榮興業公司製 * 4 莎布公司製（森之韦） (製法） A·成分（1 )〜（1 1 )加熱混合後保持70°C。 B. 成分（12 )〜（17 )加熱混合後保持70°C。 C. A中加入B後混合，均勻地進行乳化。 D ·冷卻後，C中加入成分（1 8 )得到乳霜粉底。 (實施例1 - 8 :液態粉底） (成分） （％ ) (1 )液狀含水羊毛脂 2·0 (2 )流動石蠟 5.0 (3 )硬脂酸 2.0 (4 )十六烷醇 1.0 (5 )自己乳化型單硬脂酸甘油酯 1.0 -34- 200808332 (31) (6)對甲氧基桂皮酸-2-乙基己酯 8.0 (7 ) 4-第三丁基-4’·甲氧基· 二苯甲醯基甲烷 2.0 (8 )棕櫚酸松香油* 1 0.2 (9 )對氧基安息香酸甲酯 0.1 (10)甘油 5.0 (1 1 )三乙醇胺 1.0 (1 2 )羧基甲基纖維素 0.2 (13) B entni t e 0.5 (1 4 )白樺萃取液* 2 5.0 (1 5 )甘露糖醇* 3 0.1 (1 6 )純水 剩餘量 (1 7 )氧化鈦 6.0 (1 8 )微粒子氧化鈦 2.0 (1 9 )微粒子氧化鋅 5.0 (20 )雲母 2.0 (2 1 )滑石 4.0 (22 )著色顔料 4.0 (2 3 )白樺樹液* 4 5.0 (24 )蘆筍萃取物* 5 0.5 (2 5 )甘草萃取物* 6 0.5 (26 )香料 適量 *1 日本羅修公司製 *2 九善製藥公司製 *3 Sigma公司製 -35- 200808332 (32) * 4 莎布公司製（森之-) *5 九善製藥公司製 * 6 九善製藥公司製 (製法） A.成分（1 )〜（9 )經混合溶解。 B . A中加入成分（1 7 )〜（2 2 )後均勻混合，保持7 〇。 C.成分（1 0 )〜（1 6 )均勻地溶解，保持7 〇它。 D · C中添加B後，均勻地進行乳化。 E.使D冷卻後，添加成分（2 3 )〜（2 g )得到液態粉底。 (實施例1-9 :防曬乳液） (成分） (〇/〇 )Betula platyphylla Sukatchev v ar.  j aponica H ar a ) sap of profilaggrin or filaggrin production promoter; can be used as an involucrin production promoter; can be used as a cholesterol production promoter Use; and can be used as a cell activating agent. Further, from another point of view of the present invention, the present invention provides a method for making -8-200808332 (5) with white birch (Betula platyphylla Sukatchev var.  Japonica Hara sap to promote the production of profilaggrin or filaggrin; to promote the production of encapsulation protein (inv〇lucrin); to promote cholesterol production; and cell activation method (However, all of them remove the treatment for humans). The present invention provides a novel profilaggrin or filaggrin production promoter and encapsulation protein (inv〇iucrin) production promotion from a natural product. A cholesterol production promoter and a cell activating agent. Further, the skin external preparation containing the profilaggrin and/or the filaggrin production promoter of the present invention can promote the production of natural moisturizing factors on the skin itself, and maintain the moisturization of the skin. . Moreover, the skin external preparation containing the involucrin production promoter of the present invention can be expected to normalize the barrier function of the skin epidermis, improve the moisture retention of the skin epidermis, and achieve skin roughness. . Further, the skin external preparation containing the cholesterol production promoter of the present invention can promote the production of cholesterol to the skin itself, and as a result, the moisture can be prevented from evaporating and the skin can be kept moist. Further, the skin external preparation containing cell activator of the present invention can achieve an excellent skin-beautifying effect of suppressing or improving rough skin and dull skin. The invention is described in detail below. In addition, in the present specification, "~" is a number described before and after, meaning that the lower limit 値 and the upper limit 値 are included. -9- 200808332 (6) The white birch (Betula platyphylla Sukatchev var· japonica Hara ) used in the present invention is a birch family plant, and its alias is also known as white birch and geese, distributed in Honshu, Hokkaido, and Thousand Islands, east of Gifu Prefecture. The leaves of Sakhalin, North Korea, China, and Siberia, the birch sap used in the present invention, are only bloomed and opened in early spring! During the month, the sap overflowed from the tree trunk. The component of the sap contains minerals, sugars, organic acids, amino acids, and the like. Further, the cultivation method or the production place is not particularly limited in the present invention. The preparation method of the birch sap liquid used in the present invention is not particularly limited. However, as an example of the preparation method used in the present invention, the sap taken from the trunk is heated and sterilized at 90 ° C for 10 minutes, and then cooled and filtered. Placed at 5 °C for 3 days, filtered and precipitated, etc. In addition, the sale of the birch sap can be used, and as a sales item, Mori- (made by Sabu Co., Ltd.), etc., and the filaggrin and the profilaggrin are described. The filaggrin is a pro-drug with a molecular weight of more than 300,000, and the pro-aggregin is an intermediate metabolite in the process of decomposing to a natural moisturizing factor such as an amino acid. The profilaggrin is that the epidermal granule cells are decomposed by proteolytic enzymes along with the movement of the keratinocytes, and finally become a natural moisturizing factor that maintains the skin epidermal moisturization (dermatology Mook, No.  1 5 (1 9 8 9 ), pp. 49-5 5). Therefore, in order to maintain the moisturization of the skin, it is necessary to contain profilaggrin and/or filaggrin. The present invention promotes the production of protomeric -10- 200808332 (7) angular fibrin (profilaggrin) and/or filaggrin by using birch sap. The involucrin is a C-precursor protein that forms an insoluble membrane around the cell by cross-linking with epidermal keratinocytes by transglutaminase. The cell membrane of the involucrin is combined with a part of the cell membrane of the intercellular lipid to form a layer barrier function. That is, in the formation of the barrier function, not only the cytochrome of the intercellular lipid, but also the presence of the membrane protein involucrin which is associated with it is very important. The present invention promotes the production of involucrin by using birch sap. Cholesterol is a constituent of corneal intercellular lipids (a component that occupies about 25% of the intercellular lipids), which exhibits a function of preventing evaporation of moisture from the epidermis. Further, it is known that aged skin has a function of producing cholesterol. To maintain moist skin, it is necessary to promote the production of cholesterol containing intercellular lipids. The present invention promotes the production of cholesterol contained in intercellular lipids by using birch sap. _ In addition, the decrease in cellular metabolic function caused by the increase in age is known to cause a decrease in the skin's metabolic rate or rough skin, or to cause a reduction or modification of the dermal matrix such as collagen or elastin, and wrinkles. Or related to skin elasticity and other phenomena. Further, since the waste cannot be discharged smoothly, it becomes a cause of pigmentation or dull skin. To prevent or improve these phenomena, activation of cells must be obtained. The present invention is to activate cells by using birch sap. -11 - 200808332 (8) The pro-aggregated microfilament protein (profi 1 aggri η ) or the filaggrin production promoter, the involucrin production promoter, and the cholesterol production promoter, The use of the cell activating agent is not particularly limited, and when the birch sap from the natural product is used as an active ingredient, the safety is good, and it is preferable from the related viewpoint that it can be used as a medicinal component of the external preparation for skin. The content of the medicinal agent of the present invention for the external preparation for skin is not particularly limited, but the active ingredient has a birch sap content of 0. 000 1 to 50 mass. /〇 (the following is only expressed by "%"), preferably 0. 001~30%. Only within this range, the sap can be contained stably, and has a higher pro-aggregation or filaggrin production promotion, involucrin production promotion, and cholesterol production. Promoting action, or cell activating effect, thereby achieving a moisturizing effect and a sustained effect. In addition, when the birch sap used as a medicinal agent in the present invention is added to an external preparation for skin, it can be used in combination with various other medicinal agents commonly used for external preparations for the skin, for example, one or more selected from the group consisting of antioxidants and anti-inflammatory agents. The agent, the cell activating agent, the ultraviolet ray preventing agent, the moisturizing agent, the blood circulation promoting agent and the like can further improve the effects of the present invention. Specific examples of these medicinal agents include the following. Wherein "derivative" is a salt or salt which may be formed. Further, sometimes a substance has a plurality of pharmacological effects, and therefore, a plurality of types of pharmacodynamic agents are described by one name. (In addition, in the case of plants, the name of the Chinese medicine of the alias is described in parentheses). Examples of the antioxidant include vitamin E and its derivatives, vitamin C and its derivatives, carotenoids, dibutylhydroxytoluene, butylated hydroxybenzo-12-(9) (9) 200808332 ether, rutin and their Derivatives, glutathione and its derivatives, superoxide dismutase, mannitol, spatholobus extract, Nepal geranium extract, crabwood extract, peony extract, ginkgo extract, gold flower Extract, rose (Hawthorn, Pear) extract, P.  Tormentilla extract, grape extract, cornflower extract, strawberry saxifrage extract, rosemary (R. Officinalis) extracts, tea extracts (oolong tea, black tea, green, etc.). Examples thereof include an anti-inflammatory agent, glycyrrhizic acid and derivatives thereof, glycyrrhetinic acid and derivatives thereof, sulfur and derivatives thereof, aloe extract, tomorrow leaf extract, castor extract, and Artemisia capillaris extract. , love gold extract, yellow pineapple (cork) extract, small forsythia extract, chamomile extract, healing power (fin glass grass) extract, honeysuckle (honeysuckle) extract, and blue mustard extract, rat tail Sage extract, eucalyptus extract, perilla extract, birch extract, elderberry extract, cattail extract, sapindus extract, eucalyptus extract, perennial extract, lotus leaf Extracts, red bamboo extract, zinc oxide and the like as a cell activator include vitamin A and its derivatives, asparagus extract, soybean extract, jujube (jujube) extract, garlic extract, ginseng extract, Shell extract, royal jelly, yeast extract, microbial fermentation metabolite, Ganoderma lucidum extract, and the like. Examples of the ultraviolet ray preventive agent include p-methoxycinnamic acid-2-ethylhexyl ester, 4-tert-butyl-4'-methoxybenshydrylmethane, hydroxybenzophenone and derivatives thereof. Titanium oxide, fine particle titanium oxide, zinc oxide, and fine particle zinc oxide-13-200808332 (10). When inorganic powders such as titanium oxide and zinc oxide are used, fine particles can be used. Examples of the humectant include glycerin, 1,3-butylene glycol, protein or derivatives or hydrolyzates thereof, and these salts (collagen, elastin, etc.), hyaluronic acid and salts thereof, amino acids and the like. Derivatives, sugars, D-pantothenic acid and its derivatives, glycolipids, phospholipids, celecoxime, urea, mucin, almond extract, avocado extract, genus sunflower extract, hot spring water, aloe extract , geranium extract, stalk flower extract, wild oat extract, licorice, licorice, liqnilitin, isoliquilitin and licorice extract containing the same, sorghum seed extract, Sophora flavescens extract , Gardenia Extract, Grapefruit Extract, and Mustard Seed Extract, Gentian Extract, Gentian Geranium Extract, Burdock Extract, Wheat Extract, Capsaicin Extract, Cactus Extract, Stone Alkali extract, hawthorn extract, rehmannia extract, lower grass extract, ginger extract, malva flower (thin red sunflower) extract, mulberry (mulberry white) extract, silver thyme (thyme) )Extracts Cordyceps Extract, Houttuynia Extract, Peppermint Extract, Coix Seed Extract, Witch Hazel Extract, Rose Extract, 'Clam Extract, Coltsfoot Extract, Grape Extract, Candied Date Extract, Loofah Extract, Linden extract, hop extract, pine extract, alfalfa extract, horse chestnut extract, lemon balm extract, cornflower extract, lily extract, lime extract, lavender extract, apple extract, Rice and rice bran extract, black currant extract, box finger extract, wild rose extract, acanthopanax extract, seaweed extract, etc. And 'as a moisturizer by skin surface closure, jojoba oil-14-200808332 (11), macadamia nut oil, olive oil, almond oil, almond oil, sunflower oil, sunflower oil, avocado oil, white awning Maya oil (Meadowfoam), oyster sauce, almond oil, eucalyptus oil, sesame oil, borage oil, cocoa butter, shea butter, etc. Examples of the blood circulation promoting agent include grapefruit extract, arnica extract, capsicum annuum, calamus extract, and r-oryzanol. These antioxidants, an anti-inflammatory agent, a cell activating agent, an ultraviolet ray preventing agent, a moisturizing agent, and a blood circulation promoting agent may be used in combination of one or more of them. Further, in addition to the above-mentioned components, the skin external preparation containing the medicinal agent of the present invention may be added to various components such as a cosmetic, a pharmaceutical external product, or an external pharmaceutical product, that is, water, an alcohol, a polyhydric alcohol, or an oil. The surfactant, the viscosity-increasing agent, the powder, the chelating agent, the bactericide, the enzyme, the pH adjuster, the extract of the automatic plant, the microorganism, the fragrance, and the like can be appropriately added within a range not impairing the effects of the present invention. The example of the form of addition of the external preparation for skin containing the medicinal agent of the present invention is not particularly limited, and for example, it can be selected from the group consisting of an emulsion, a cream, a lotion, a beauty liquid, a mask, a detergent, a makeup, and a dispersion. A cosmetic such as a liquid, an ointment, a liquid, an aerosol, a patch, or the like, or a pharmaceutical for external use. [Embodiment] [Examples] Next, the production examples, test examples, and examples will be described more specifically in the present invention -15-200808332 (12), but the present invention is not limited thereto. Test Example 1: The amount of profilaggrin and/or filaggrin produced by the test was used as a control group of the birch sap (trade name "Senzhi", manufactured by Sabu Co., Ltd.). It is known that there is a calcium solution (calcium chloride solution; hereinafter referred to as "calcium solution") which can enhance the differentiation promoting effect on human epidermal cells, and, in addition, only the growth medium is used as a control group, and the original poly-angle is given. The role of fifigrin and/or filaggrin production was investigated. After normal human regeneration and epidermal cells are precultured, it is intended to prevent the permeation pressure of the medium from decreasing, and the amount of the culture medium is 30v/v°/. , 20v/v°/. The lOv/v%, lv/v% equivalents were added by lyophilization to a concentration of 1 / 10 0. Further, for the control group, the calcium ion concentration in the original medium added to the calcium solution was adjusted to 0. 03 mM / mL and the total amount is 1. After 5 mM/mL, cultured for one week under each medium condition, the medium was taken out, washed once with PBS (-), and the epidermal cells were recovered. This added 1 OmMTris-NaCl buffer (containing 8 M urea, 1 v/v% Triton X-100, protease inhibitor cocktail) (adjusted EDTA, PMSF, NMF to ρΗ7·5) to the recovered cells. After the final concentration is 1 m Μ )), the cells are disrupted by ultrasonic treatment at 4 ° C for 1 〇 minutes, and the cell residue is removed by centrifugation, and the protein is quantified. The amount of protein in each sample is determined to be 4 Samples were processed in ~12% polyacrylamide gel for SDS-PAGE. After the end of the electrophoresis, the protein was transcribed on the nitrocellulose membrane, and the profilaggrin and/or poly-microfilament protein were detected by Western blotting method - 16-200808332 (13). Filaggrin). The primary antibody was a monoclonal antibody (manufactured by Biomedical Technologies) using mouse anti-human filaggrin (filaggrin), and the secondary antibody was labeled with IgG (Amersham) using anti-mouse peroxidase by chemiluminescence method. Test. One of the detected sections was read by a fluorometer, and the area was measured as the amount of production. The amount of production for the control group is shown in Table 1. [Table 1] The sample name of the original poly-angle microfilament protein and / or poly-angle microfilament protein production energy (control group is 100) Birch sap 30v / v% equal amount of 1099 birch sap 20v / v% equal amount of 12 16 white birch Sap 10Wv% equal addition 565 Birch sap lv/v% equal addition 216 Calcium 388 It is known from Table 1 that birch sap and calcium have both promoted profilaggrin and/or poly-microfilament protein ( The role of filaggriii). It is shown to be poor for the 1 v/v% concentration of the broccoli, and the higher the concentration of profilaggrin and/or fiiaggriri in the above test concentrations. effect. Therefore, the 'brown sap sap is equivalent to or higher profilaggrin and/or polyglycoside, which is known to promote the production of profilaggrin and/or filaggrin. -17- 200808332 (14) Filaggrin produces a promoting effect. Test Example 2: Determination of the production amount of inv〇lucrin as a control group of birch sap, using a calcium solution (calcium chloride solution) which is known to have a high differentiation promoting effect on human epidermal cells; The solution ") was used as a control group only for the growth of the medium, and the effect of the amount of involucrin produced was investigated. After pre-culture of normal human neonatal epidermal cells, in order to prevent the hypotonic pressure of the medium from decreasing, the amount of the medium is 30v/v%, 20v/v°/〇, 10v/v%, lWv%, etc. after lyophilization. Concentrated to 1/10 times the birch sap. Further, the calcium solution was added to the control group to the concentration of calcium ions contained in the original medium. 0 3mM/mL makes the total amount 1 . After 5 mM/mL, cultured for one week under each medium condition, the culture medium was taken out and washed once with PBS (-), and the epidermal cells were collected. Secondly, add PH7 to the recovered cells. 5 of 1 OmMTris-NaCl buffer (containing 8 Μ urea, 1 v/v% Triton X-1 〇〇, protease inhibitor cocktail (adjusting each EDTA, PMSF, NMF final concentration of 1 mM)) , " Ultrasonic treatment at 4 ° C for 1 〇 minutes to break the cells, centrifuged to remove cell debris after protein quantification, want to make the protein content of each sample is fixed, 4~12% of polypropylene 醯The amine gel was sampled and subjected to SDS-PAGE. After the end of the electrophoresis, the protein on the gel was transcribed on the nitrocellulose membrane, and the involucrin was detected by Western blotting. The primary antibody was a monoclonal antibody (manufactured by Abeam Co., Ltd.) from mouse using anti-human crust protein (involucrin), and 2 times -18-(15) (15)200808332 antibody was labeled with anti-mouse peroxidase IgG (Amersham Co., Ltd.) The system is detected by chemiluminescence. The detected section was read by a fluorometer, and the area was measured as the amount of production. The amount of production for the control group is shown in Table 2. [Hol 2] The name of the shell protein production energy (control group is 100) Birch sap 3〇v/v% equal addition 1 57 White birch sap 2〇v/v°/〇 equal addition 154 Birch sap 1 0 v / v ° / 〇 equal addition 146 birch sap 1 v / v% equal addition 127 calcium 128 It is known from Table 2 that birch sap and calcium have the effect of promoting the production of involucrin, for calcium, birch sap It has a higher promoting effect on the involucrin. Therefore, it can be confirmed that the white birch sap has been known to promote the production of involucrin, and the promotion of involucrin is more excellent. Test Example 3: Determination of cholesterol production rate as a control group of birch sap, used A calcium solution (calcium chloride; hereinafter, simply referred to as "calcium solution") which enhances the differentiation promoting action of human epidermal cells is known, and only the growth in the medium is used as a control group, and the effect of imparting cholesterol is investigated. After pre-culture of normal human neonatal epidermal cells -19-200808332 (16), to prevent the medium's osmotic pressure from decreasing, add 30v/v%, 20v/v%, 10v/v%, lv/v for the medium. % equal amounts were lyophilized and concentrated to 1 / 10 times the white birch solution. Further, a calcium solution was added to the control group to add a calcium ion concentration of 0 in the original medium. 03 mM / mL to make the total amount of 1. After 5 mM/mL, cultured for one week under each medium condition, the medium was taken out and washed once with PB S (-), and the epidermal cells were recovered. Adding water, chloroform and sterol to the human epidermal cells. After 5 mL and mixing, the chloroform layer was separated and dried to solidify. The extracted lipid is HPTLC disk [矽G60 (20x10cm) Art. 5 64 1 ; Merck & Co., Ltd., developed twice with a mixed solvent (chloroform: methanol: acetic acid = 190: 9:1). After spraying 40% sulfuric acid, it was heated at 180 degrees for 15 minutes, and then colored, and the cholesterol on TLC was colorimetrically measured by a fluorescence meter. The control group grown only in the medium was used as 1 · 〇, and the cholesterol production promotion rate was calculated. The results are shown in Table 3. [Table 1] Sample name cholesterol production energy (control group is 100) Birch sap 3 0 v / v % equal amount of 125 white birch sap 2〇v/v% equal amount 123 addition Birch sap 1 0 v / v % equal addition 117 Birch sap 1 v/v% equal addition 108 Calcium 105 It is known from Table 3 that birch sap and calcium have the effect of promoting cholesterol production, but for calcium, birch sap is higher in all tested concentrations-20- 200808332 (17) Cholesterol production promoting effect. Therefore, the birch sap has a higher cholesterol-promoting effect than calcium which has been known to promote cholesterol production. Test Example 4: Promotion of keratinization of epidermal keratinocytes Normal human neonatal skin epidermal keratinocytes (manufactured by KURABO Co., Ltd.) were cultured on a diameter of 24 multi-grid plates using a culture medium for epidermal keratinocyte proliferation (manufactured by KURABO Co., Ltd.). The cells were sown at 6000 cells/division and cultured at 37 t for 24 hours in a 5% vol carbon dioxide atmosphere. After the cultivation, the birch sap solution was added to a final concentration of 1% by weight (Example 1), the comparative example was no addition (Comparative Example 1), and the calcium chloride was added as a positive target to a final concentration of 1. An example of 5 mM (Comparative Example 2) was cultured for 3 days. After the cultivation, the cells of each sample were photographed, and the morphology thereof was examined. The photographs taken are shown in Figures 1 to 3. Further, the number of viable cells was calculated by MTT (3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazorium bromide) assay. The result is shown in Figure 4. As shown in Figs. 1 to 3, examples of adding a birch sap solution to each cell form (Example 1 : Fig. 1) and an example of adding calcium chloride (Comparative Example 2: Fig. 3) and no addition of these components were added. Comparative Example (Comparative Example 1: Fig. 2) Comparison showed that hypertrophy and re-stratification of cells revealed that keratinization was promoted. The keratinization of the cells is such that the faster the metabolism of the skin, that is, the higher the metabolic function is promoted, the more the keratinization is promoted, and the higher the cell activation is. Fig. 4 is a graph showing the number of viable cells of each sample. The general cells that will breathe will stop breathing due to keratinization. If the keratinocytes are not peeled off, the oxygen consumption of the cultured cells will be reduced. Therefore, the number of viable cells can be calculated by quantifying the amount of oxygen consumed. -21 - 200808332 (18) The less the number of viable cells, the more keratinization is promoted. From the results of Fig. 4, it was found that the addition of each of the g-types of the birch chlorochloride (comparative example 1 and comparative example 2) compared with the comparative example 1) showed a more keratinization-promoting effect of the number of viable cells. Test Example 5: Cell proliferation promoting action The cell viability of human new (NB1RGB) was measured using the MTT assay method. MTT is related to the amount of NADH which has an absorption wave at 5 70 nm due to the enzyme (NAD Η ) reaction in the granule. Therefore, the soluble wax can be determined by measuring the absorbance. The number of fine cells. The appropriate amount of the medium was taken out from the 24 microplates, and the bud cells were seeded. The carbon dioxide concentration in the 7 7 t was replaced by the pure water used in the white birch box (made by Nishui Co., Ltd.), and diluted with ordinary MEM medium for two days. The medium with a birch sap content of 75 ° / 〇 ((Example 3) was used in the general culture as a control group (Comparative Example 3). The sample was added on the fourth day of culture. The next day, MTT (SIGMA public 1 will be used) The prepared solution 1 〇〇# L is added to each cell.; that is, the sample containing the higher fine liquid and the known keratinizing agent and the non-added sample (reduced, the line bud cells having the same degree of birth are inserted into the living cells, Reducing cracking with the deposit, it is known that these formations are produced by the method of cell-energy metabolism or from human newborns (5% in place). Another [manufactured Ig MEM is used to make a dilution series. In the second embodiment, 2), 100% of the nutrient is cultured, and the medium is changed, and 3 is added again.) 5 mg/mL is cultured for 4 hours, -22-200808332 (19) The medium is removed, and the solution is dissolved in isopropanol. Generated wax. Using a micro analyzer, 6 3 0 nm was used as a reference 値, and the absorbance at 570 nm was measured'. The ratio to the control was calculated. The results are shown in Fig. 5. From the results shown in Fig. 5, the sample added with the birch sap was promoted in a concentration-dependent manner. In the above, it is confirmed that the birch sap liquid has a cell-promoting effect for promoting cell proliferation. Examples of skin external preparations containing various forms of the medicinal agents of the present invention are exemplified below. Further, Examples 1-1 to -9 contain Examples of skin external preparations of pro-aggregated fibrin (^ profilaggrin) or filaggrin-producing agents, and examples 2-1 to 2-9 are produced by containing involucrin of the present invention. Examples of the external preparation for skin of the accelerator, Examples 3-1 to 3-9 are examples of the external preparation for skin containing the cholesterol production promoter of the present invention, and Examples 4-1 to 4-9 are for the administration of the cells containing the present invention. An example of a skin external preparation for the agent (Example 1 _ 1 : lotion) (ingredient) (%) (1) glycerin 3. 0 (2) 1,3-butanediol 2. 〇 (3) Propylene glycol _ 4. 〇 (4) oleyl alcohol 〇 .  (5) Single lauric acid polyethylene oxide (20E. O·) Sorbitol 15 (6) Polyethylene oxide (20E. O. ) lauryl ether 〇. 5 (7) Thirty Carbon Court 0. 5 -23- 200808332 (20) (8) Ethanol 15. 0 (9) acetic acid dl-α-tocopherol* 1 0. 5 (1 〇 ) Spice Appropriate amount (1 1 ) Dipotassium glycyrrhizinate* 2 0. 5 (12) Rosemary Extract* 3 0. 5 (13) dl-pyrrolidone carboxylic acid sodium solution* 4 0. 2 (1 4 ) Locust bean gum 1. 0 (15) Coix seed extract* 5 0. 5 (16) Birch sap *6 20. 0 (1 7 ) Remaining amount of pure water * 1 Eisai company *2 Manufactured by Jiu-Gang Pharmaceutical Co., Ltd. *3 manufactured by Jiu-Sen Pharmaceutical Co., Ltd. * 4 Ajinomoto Co., Ltd. *5 Nine-good pharmaceutical company *6 Saab company system Debate) (Method) A·Components (1) to (10) are dissolved by mixing. B · The components (1 1 ) to (1 7 ) are dissolved by mixing. C.  After adding B to A, it is uniformly mixed to obtain a lotion. (Example 1-2: Cream) (Component) (%) -24- 200808332 (21) (1) Decanecane 20. 0 (2) beeswax 5. 0 (3) jojoba oil 5. 0 (4) glyceryl monostearate 2. 0 (5) monostearic acid polyethylene oxide (20E. O·) sorbitol 2. 0 (6) sorbitan monostearate 2. 0 (7) plant alcohol * 1 0. 5 (8) Candelilla 1. 0 (9) glycerol 5. 0 (1 〇 ) p-oxybenzoic acid methyl ester 0. 1 (1 1 ) Lily Extract * 2 1. 0 (1 2 ) ginseng extract * 3 1. 0 (1 3 ) Ginkgo Biloba Extract * 4 1. 0 (1 4 ) jujube extract * 5 1. 0 (1 5 ) Sophora flavescens extract * 6 1. 0 (16) Hydrolyzed rice extract *7 1. 0 (1 7 ) Licorice Extract * 8 1. 0 (1 8 ) Angelica extract * 9 1. 0 (19) Birch sap *10 5. 0 (2 0 ) Remaining amount of pure water (2 1 ) Proper amount of 氺1 Eisai company* 2 Manufactured by Jiuzhan Pharmaceutical Co., Ltd. *3 - 9-22 (22) (22)200808332 * 4 Phytochemicals公司 公司 制药 制药 九 九 九 九 九 九 九 九 九 九 九 九 九 九 TECH TECH TECH TECH TECH TECH TECH TECH TECH TECH TECH TECH TECH 九 九 九 九 九 九 九 九 九 九 九 九 九 九 九 九 九 九 九 九 九 九 九 九(Method of production) A. The components (1) to (10) were kept at 70 ° C after heating and mixing. B·Components (11) to (20) were kept at 70 ° C after heating and mixing. C. Emulsification was carried out after adding B to A. D. After cooling, the component (2 1 ) is added to C to obtain a cream. When both of Example 1-1 and Example 1-2 are used on the skin, the promotion of profi lag grin and/or filaggrin can produce skin itself. A natural moisturizing factor, so it is a lotion and cream that can maintain the moisturization of the skin sustainably (Example 1-3: Cleansing Cream) (Ingredient) (%) (1) Paraffin 10. 0 (2) beeswax 3. 0 -26- 200808332 (23) (3) Vaseline 1 5 · 0 (4) mobile paraffin 41. 0 (5) sorbitan sesquioleate 4. 2 (6) monooleic acid polyethylene oxide (20E. O·) sorbitol 0. 8 (7) Hydrogenated Soy Phospholipid 〇·5 (8) White Mango Oil (Meadowfoam) *1 1. 0 (9) p-oxybenzoic acid methyl ester 0. 1 (1 〇 ) Wenyu Seed Extract* 2 1. 0 (1 1 ) Equilibrium Extract * 3 1. 0 (12) Large flower sunflower extract *4 1. 0 (13) Birch sap*5 5. 0 (1 4 ) Remaining amount of pure water (1 5 ) Proper amount of fragrance * 1 Japanese surfactant company*2 香荣兴业公司*3 九善制药股份有限公司* 4 九善制药股份有限公司*5 莎布公司制制(森之-) (Method) A·Component (1)~(9) After heating and mixing, keep 7 (TC. B.  The components (10) to (14) were heated and mixed, and kept at 70 °C. C.  After adding B to A, it was mixed and emulsified uniformly. D.  After cooling, the component (15) is added to C to obtain a cleansing cream. -27- 200808332 (24) When used in the skin, the 1-3 can be promoted by profilaggrin and/or filaggrin, and the skin itself is naturally produced. A moisturizing factor, so it is a cleansing cream that maintains smooth skin. (Example 1-4: Emulsion) (ingredient) (%) (1) Sorbitan monostearate 0. 3 (2) monooleic acid polyethylene oxide (20E. O. ) sorbitol 〇·1 (3) lipophilic glyceryl monostearate 0. 2 (4) stearic acid 0. 5 (5) cetyl alcohol 0. 5 (6) triacontane 3. 0 (7) Mobile sarcophagus 4. 0 (8) vegetal triacontan* 1 0. 2 (9) Tri-2·ethylhexanoate 2. 0 (10) dimethyl polyoxane 1. 0 (1 1 ) hydrogenated soybean phospholipid 〇·1 (12) niacin dl-α-tocopherol*2 0. 05 (1 3 ) p-oxybenzoic acid ethyl ester 0. 1 (14) Carboxyvinyl polymer 0. 1 (1 5 ) sodium hydroxide 0. 05 (1 6 ) glycerol 5. 0 -28- 200808332 (25) (17) 1,3-butanediol 7·0 (1 8) ethanol 5. 0 (1 9 ) Birch sap* 3 10. 0 (20) Peony Extract *4 2. 0 (21) Ganoderma lucidum extract *5 2. 0 (2 2 ) sodium hyaluronate 1% aqueous solution * 6 5. 0 (23) Ethyl glutamic acid * 7 0. 3 (24) pure water remaining amount (25) porous ceria 3. 0 (26) Proper amount of spices*1 Manufactured by Nikko Chemical Co., Ltd. 2 Eisai Co., Ltd.*3 Saab Co., Ltd. (Mori no 雫) *4 Manufactured by Jiu-Gang Pharmaceutical Co., Ltd. * 5 manufactured by Jiu-Gang Pharmaceutical Co., Ltd. *6 Sunlight Chemical Co., Ltd.* 7 Co., Ltd. (manufacturing method) A·Components (14) to (23) were heated and mixed, and kept at 70 °C. B·Components (1) to (13) were kept at 70 ° C after heating and mixing. C. After adding B to A, it was mixed and emulsified uniformly. After D·cooling, ingredients (24), (25), and (26) are added to C to obtain an emulsion. -29- 200808332 (26) (Example 1-5: Emulsion) (ingredient) (%) (1) Sorbitan monostearate 0. 3 (2) monooleic acid polyethylene oxide (20E. O. ) sorbitol 0. 1 (3) lipophilic glyceryl monostearate 0. 2 (4) stearic acid 0. 5 (5) Sixteen hospitals alcohol 0. 5 (6) 30 carbon 垸 3. 0 (7) mobile paraffin 4. 0 (8) tris-2-ethylhexanoic acid glyceride 2. 0 (9) dimethyl polyoxane 1. 0 (1 〇 ) hydrogenated soybean phospholipids 0. 1 (1 1 ) rutin* 1 0. 2 (12) p-oxybenzoic acid methyl ester 0. 1 (13) Carboxyvinyl polymer 0. 1 (1 4 ) sodium hydroxide 0. 05 (15) Glycerin 5. 0 (16 ) 1, 3-butanediol 7. 0 (17) Birch sap*2 3. 0 (1 8 ) pure water remaining amount (1 9 ) ethanol 5. 0 (20) Yeast Extract *3 0. 5 (2 1 ) tomorrow leaf extract * 4 0. 5 -30- (27) (27)200808332 (2 2 ) Hop Extracts * 5 〇 5 (23 ) Peach Extracts * 6 〇 5 (2 4 ) Porous Ceria 3. 〇(25)The right amount of spices* 1 Sigma company* 2 Shabu company system (Sengzhi-) * 3 BIO-DELL company system*4 Jiushan Pharmaceutical Co., Ltd.*5 Jiushan Pharmaceutical Co., Ltd.*6 Jiushan Pharmaceutical Co., Ltd. (Manufacturing method) A·Components (13) to (23) were heated and mixed and kept at 70°C. (: B. The components (1) to (12) are heated and mixed to maintain 70. (: C. After adding B to A, it was mixed and emulsified uniformly. D. After cooling, ingredients (24) and (25) are added to C to obtain an emulsion. When both of the examples 1-4 and the examples 1-5 are used on the skin, the skin can be promoted by the pro-aggregated microfilament protein (pr 〇fi 1 aggri η ) and/or the filaggrin. It produces a natural moisturizing factor, so it is an emulsion that sustains the smoothness of the skin. (Example 1-6: Mask) (Component) (%) -31 - 200808332 (28) 15. 0 0. 5 0. 5 0. 2 5. 0 5. 0 remaining amount 1. 0 (1) polyvinyl alcohol (2) anhydrous citric acid (3) polyethylene glycol 1500 (4) white flower oil (Meadowfoam) *1 (5) polypropylene oxide (10 Ρ · Ο ·) methyl grape Glycoside (6) Glycerin (7) Pure Water (8) Tea Extract * 2 (9) Essential Oil Rose (Rosarugosa Thunb) Extract *3 1. 0 (1 0) Anhydrous caffeine* 4 1. 0 (11) Birch sap*5 5. 0 (12) Phosphoric acid-L-ascorbate*6 3. 0 (1 3 ) pure water 5. 0 (1 4 ) Ethanol 18. 0 (1 5 ) p-oxybenzoic acid ethyl ester 0. 1 (1 6 ) Proper amount of fragrance * 1 Japanese surfactant company*2 Manufactured by Jiu-Gang Pharmaceutical Co., Ltd.*3 Manufactured by Jiu-Gang Pharmaceutical Co., Ltd.* 4 Pure Pharmaceutical Industry Co., Ltd.* 5 Saab Co., Ltd. (Sengzhi-) 氺6 Daylight Chemical company system (manufacturing method) -32 200808332 (29) Α·Components (1) to (7) are mixed and heated and dissolved at 70 °C. B · The components (8) to (1 3) are dissolved by mixing. C·Components (14) to (16) are dissolved by mixing. D.  After adding B to A, the mixture was mixed with C under cooling to obtain a mask. When the skin of Example 1 -6 is used on the skin, it can be promoted by the pro-aggregated microfilament protein ( ^ Profilaggrin ) and/or filaggrin to make the skin itself produce a natural moisturizing factor, so it is sustainable. A mask that keeps the skin smooth. (Example 1-7: cream foundation) (ingredient) (%) (I) sorbitan sesquioleate 3. 0 (2) Self-emulsified glyceryl monostearate 2. 0 (3) aluminum stearate 0. 5 (4) Pure ground 鱲 5. 0 (5) 30-carbon institute 10. 0 (6) Flowing paraffin 15. 0 (7) Vaseline 5. 0 (8) titanium oxide 8. 0 (9) talc 4. 0 (1 〇 ) coloring pigment appropriate amount (II) methyl benzoic acid methyl ester 0. 05 (1 2 ) Propylene glycol 5. 0 -33 200808332 (30) (1 3 ) Honeysuckle Extract * 1 1. 0 (14) White birch extract *2 1. 0 (1 5 ) Horse chestnut extract * 3 1. 0 (1 6 ) Birch sap* 4 10. 0 (1 7 ) Remaining amount of pure water (1 8 ) Proper amount of spices *1 manufactured by Nagaoka Spice Co., Ltd. * 2 manufactured by Jiu-Gang Pharmaceutical Co., Ltd. *3 manufactured by Fraser Co., Ltd. * 4 Saab Co., Ltd. (Sen Wei) (Method) A - The components (1) to (1 1 ) were kept at 70 ° C after heating and mixing. B.  The components (12) to (17) were kept at 70 ° C after heating and mixing. C.  After adding B to A, it was mixed and emulsified uniformly. D · After cooling, the component (18) is added to C to obtain a cream foundation. (Examples 1 - 8 : Liquid Foundation) (Component) (%) (1) Liquid aqueous lanolin 2·0 (2) Flowing paraffin 5. 0 (3) stearic acid 2. 0 (4) cetyl alcohol 1. 0 (5) Self-emulsified glyceryl monostearate 1. 0 -34- 200808332 (31) (6) p-Methoxycinnamate-2-ethylhexyl ester 8. 0 (7) 4-t-butyl-4'.methoxy-diphenylmethylmethane methane 2. 0 (8) palmitic rosin oil * 1 0. 2 (9) p-oxybenzoic acid methyl ester 0. 1 (10) Glycerin 5. 0 (1 1 ) triethanolamine 1. 0 (1 2 )carboxymethyl cellulose 0. 2 (13) B entni t e 0. 5 (1 4 ) White birch extract * 2 5. 0 (1 5 ) mannitol* 3 0. 1 (1 6 ) pure water remaining amount (1 7 ) titanium oxide 6. 0 (1 8 ) microparticle titanium oxide 2. 0 (1 9 ) micronized zinc oxide 5. 0 (20) mica 2. 0 (2 1 ) talc 4. 0 (22) coloring pigment 4. 0 (2 3 ) Birch sap* 4 5. 0 (24) Asparagus extract * 5 0. 5 (2 5 ) Licorice Extract * 6 0. 5 (26) Proper amount of spices*1 Made by Japan Rosho Corporation*2 Manufactured by Jiushan Pharmaceutical Co., Ltd.*3 Sigma-35-200808332 (32) * 4 Shabu Company (Senzhi-) *5 Nine-good Pharmaceutical Co., Ltd. * 6 Jiushan Pharmaceutical Co., Ltd. (Manufacturing Method) A. The components (1) to (9) are dissolved by mixing. B.  Add the ingredients (1 7 ) to (2 2 ) in A and mix them evenly, keeping 7 〇. C. The ingredients (1 0 ) to (1 6 ) were uniformly dissolved and kept at 7 〇. After adding B to D · C, emulsification was performed uniformly. E. After cooling D, the components (2 3 ) to (2 g ) were added to obtain a liquid foundation. (Example 1-9: sunscreen lotion) (ingredient) (〇/〇)

(1 )聚氧伸烷基變性有機聚矽氧烷 i Q (2)二甲基聚矽氧烷 5.0 (3 )八甲基環四矽氧烷 2〇 〇 (4) 異壬酸異十三院酯 5.0 (5) 對甲氧基桂皮酸-2-乙基己酯 50 (6 )對氧基安息香酸甲酯 〇 . ；t (7 )聚矽氧處理微粒子氧化鈦 1〇.〇 (8 )聚矽氧處理微粒子氧化鋅 丨〇 . 〇 (9 )聚苯乙烯粉末 3.0 (1 0 )三甲基甲矽烷氧矽酸 0.5 (1 1 )二丙二醇 3.0 -36- (33) (33)200808332 (1 2 )乙醇 10.0 (1 3 )白樺樹液* i 10.0 (14 )食鹽 0.2 (1 5 )純水 剩餘量 (16)々-胡蘿菠素* 2 0.0 1 (1 7 )洋甘菊萃取物* 3 1.0 (1 8 )吾亦紅萃取物* 4 1 .0 (1 9 )香料 適量 *1 莎布公司製（森之帮） *2 Sigma公司製 *3 九善製藥公司製 氺4 九善製藥公司製 (製法） Α·混合成分（1 )〜（10)，保持70°C。 B·混合成分（1 1 )〜（15 )，保持70°C。 C · A中添加B後均勻地進行乳化。 D·冷卻後，C中加入成分（1 6 )〜（1 9 )得到防曬乳液。 實施例1 -7〜9皆使用於皮膚時，可藉由原聚角微絲 蛋白（profilaggrin )及/或聚角微絲蛋白（filaggrin )產 生促進作用，使得肌膚本身產生天然保濕因子，故其爲可 持續維持肌膚滑潤的乳霜粉底、液態粉底及防曬乳液。 -37- (34) (34)200808332 (實施例2-1 :化妝水） (成分） （％ ) (1 )甘油 3.0 (2 ) 1，3-丁二醇 2.0 (3 )丙二醇 4.0 (4 )油醇 0.1 (5 )單月桂酸聚環氧乙烷（20E.0.)山梨醇酐 1.5 (6 )聚環氧乙烷（20E.0.)月桂醚 0.5 (7 )三十碳烷 0.5 (8 )乙醇 2 0.0 (9 )對氧基安息香酸甲酯 0.1 (10)乙酸dl-α-生育醇氺1 0.5 (1 1 )甘草酸二鉀* 2 0.5 (12)迷迭香萃取物*3 0.5 (13 ) dl-吡咯烷酮羧酸鈉液* 4 0.2 (1 4 )刺槐豆膠 1.0 (1 5 )薏仁萃取物* 5 0.5 (16)白樺樹液* 6 20.0 (1 7 )純水 剩餘量 * 1 Eisai公司製 *2 九善製藥公司製 *3 九善製藥公司製 *4 味之素公司製 *5 九善製藥公司製 -38- 200808332 (35) *6 莎布公司製（森之乘） (製法） A·成分（1 )〜（10)經混合溶解。 B ·成分（1 1 )〜（1 7 )經混合溶解。 C . A中加入B後均勻混合得到化妝水。 (實施例2 - 2 :乳霜） (成分） （％ ) (1 )三十碳烷 20.0 (2 )蜜蠟 5.0 (3 )荷荷芭油 5.0 (4 )單硬脂酸甘油酯 2.0 (5 )單硬脂酸聚環氧乙烷（20E.0·)山梨醇酐 2.0 (6 )單硬脂酸山梨醇酐 2.0 (7 )植物醇* 1 0.5 (8 )堪地里蠟 1.0 (9 )甘油 5.0 (10 )對氧基安息香酸甲酯 0.1 (1 1 )百合萃取物* 2 1.0 (1 2 )人參萃取物* 3 1.0 (1 3 )銀杏萃取物* 4 1.0 (14)大棗萃取物*5 1.0 (1 5 )苦參萃取物* 6 1.0 -39- 200808332 (36) 1.0 1.0 5.0 剩餘量 適量 (1 6 )水解米萃取物* 7 (1 7 )甘草萃取物* 8 (1 8 )當歸萃取物* 9 (1 9 )白樺樹液* 1 0 (2 0 )純水 (2 1 )香料 * 1 Eisai公司製 *2 九善製藥公司製 ~ *3 九善製藥公司製 * 4 常磐植物化學硏究所公司製 *5 九善製藥公司製 *6 九善製藥公司製 氺7 TECHNO-BULL公司製 *8 九善製藥公司製 *9 日本粉末藥品公司製 *10 莎布公司製（森之-) ' (製法） A·成分（1 )〜（10 )加熱混合後保持70°C。 B·成分（1 1 )〜（20 )加熱混合後保持70°C。 C.A中加入B後進行乳化。 D ·將C冷卻後，加入成分（2 1 )後混合，得到乳霜。 實施例2-1及實施例2-2皆使用於皮膚時，可藉由包 -40- 200808332 (37) 殼蛋白（invoUcrin )產生促進作用使得肌膚表皮的阻礙 功能正常化，其爲肌膚表皮的水分保持改善效果、肌膚粗 糙防止效果優良的化妝水與乳霜。 (實施例2-3 :潔面乳霜） (成分） （％ ) (1 )石蠟 10.0 (2 )蜜蠟 3.0 (3 )凡士林 15.0 (4 )流動石蠟 41.0 (5 )失水山梨醇倍半油酸酯 4.2 (6 )單油酸聚環氧乙烷（20Ε·0·)山梨醇酐 0.8 (7) 氫化大豆磷脂質 0.5 (8) 白芒花好油 （Meadowfoam) *1 1.0 (9) 對氧基安息香酸甲酯 0.1 (1 〇 )溫桴種子萃取物* 2 1.0 (1 1 )石鹼草萃取物* 3 1.0 (12)大花葵花萃取物*4 1.0 (1 3 )白樺樹液* 5 5.0 (1 4 )純水 剩餘量 (1 5 )香料 適量 * 1 日本界面活性劑公司製 *2 香榮興業公司製 *3 九善製藥公司製 -41 - 200808332 (38) * 4 九善製藥公司製 * 5 莎布公司製（森之—） (製法） A·成分（1 )〜（9 )加熱混合後保持7(KC。 B·成分（10)〜（14)加熱混合後保持70。(：。 C. A中加入B後混合，均勻地進行乳化。 D. 將C冷卻後，加入成分（1 5 )後混合得到潔面乳霜。 貫施例2-3使用皮膚時’可藉由包殻蛋白（inv〇iucrin )產生促進作用使得肌膚表皮的阻礙功能正常化，其爲肌 膚表皮的水分保持改善效果、肌膚粗糙防止效果等優良之 潔面乳霜。 (實施例2-4 :乳液） (成分） （％ ) (1 )單硬脂酸山梨醇酐 0.3 (2 )單油酸聚環氧乙烷（20E.0·)山梨醇酐 〇·1 (3)親油型單硬脂酸甘油酯 〇·2 (4 )硬脂酸 〇·5 (5 )十六烷醇 〇·5 (6 )三十碳烷 3.0 (7 )流動石鱲 4.0 (8 )植物性三十碳烷* 1 〇.2 •42- (39) (39)200808332 (9 )三-2-乙基己酸甘油酯 2·0 (10) 二甲基聚矽氧烷 1.0 (11) 氫化大豆磷脂質 〇·1 (12) 煙酸dl-α-生育醇*2 0.05 (1 3 )對氧基安息香酸乙酯 0.1 (14)羧基乙烯聚合物 0.1 (1 5 )氫氧化鈉 0.05 (1 6 )甘油 5.0 (17 ) 1，3-丁二醇 7.0 (1 8 )乙醇 5.0 (19)白樺樹液*3 10.0 (20 )芍藥萃取物* 4 2.0 (21)靈芝萃取物*5 2.0 (2 2 )透明質酸鈉1 %水溶液* 6 5.0 (23)乙醯基麩胺酸*7 0.3 (2 4 )純水 剩餘量 (25 )多孔質二氧化矽 3.0 (26 )香料 適量 * 1 日光化學公司製 *2 Eisai公司製 * 3 莎布公司製（森之零） * 4 九善製藥公司製 * 5 九善製藥公司製 *6 日光化學公司製 -43- 200808332 (40) * 7 協和發酵公司製 (製法） A.成分（14)〜（24)加熱混合後保持70°C。 B·成分（1 )〜（13)加熱混合後保持70°C。 C.A中加入B後混合，均勻地進行乳化。 D·將C冷卻後，加入成分（25 ) 、（ 26 )混合得到乳液。 (實施例2-5 :乳液） (成分） （％ ) (1 )單硬脂酸山梨醇酐 0.3 (2 )單油酸聚環氧乙烷（20E.0·)山梨醇酐 0.1 (3 )親油型單硬脂酸甘油酯 0.2 (4 )硬脂酸 0.5 (5 )十六烷醇 0.5 (6 )三十碳烷 3.0 (7 )流動石蠟 4.0 (8 )三-2-乙基己酸甘油酯 2.0 (9)二甲基聚矽氧烷 1.0 (1 〇 )氫化大豆磷脂質 〇·1 (1 1 )芸香苷* 1 0.2 (12 )對氧基安息香酸甲酯 0.1 (1 3 )羧基乙烯聚合物 0.1 (1 4 )氫氧化鈉 0.05 -44 - 200808332 (41) (1 5 )甘油 5.0 (16) 1，3-丁二醇 7.0 (17) 白樺樹液*2 3.0 (1 8 )純水 剩餘量 (1 9 )乙醇 5.0 (20 )酵母萃取物* 3 0.5 (2 1 )明日葉萃取物* 4 0.5 (22 )蛇麻草萃取物* 5 0.5 (23 )桃子萃取物* 6 0.5 (24 )多孔質二氧化矽 3.0 (2 5 )香料 適量 * 1 Sigma公司製 *2 莎布公司製（森之雫） 氺3 BIO-DELL公司製 * 4 九善製藥公司製 *5 九善製藥公司製 *6 九善製藥公司製 (製法） A. 成分（13 )〜（23 )加熱混合後保持70°C。 B. 成分（1 )〜（12 )加熱混合後保持70°C。 C. A中加入B後混合，均勻地進行乳化。 D·將C冷卻後，加入成分（24 ) 、（ 25 )混合得到乳液。 -45- (42) (42)200808332 實施例2-4及實施例2-5皆使用於皮膚時，可藉由包 殼蛋白（invoUcrin )產生促進作用而使肌膚表皮的阻礙 功能正常化，其爲肌膚表皮的水分保持改善效果、肌膚粗 糙防止效果優良的乳液。 (實施例2-6 :面膜） (成分） （％ ) (1 )聚乙烯醇 15.0 (2 )無水矽酸 0.5 (3 )聚乙二醇 0.5 (4) 白芒花好油 （Meadowfoam) *1 0.2 (5) 聚環氧丙烷（1 0P.〇.)甲基葡萄苷 5.0 (6 )甘油 5.0 (7 )純水 剩餘量 (7) 茶萃取物*2 1.0 (8) 精油玫瑰（R〇SarugosaThunb)萃取物 *3 1.0 (9 )無水咖啡因* 4 1.0 (1 0 )白樺樹液* 5 5 · 0 (11)磷酸-L-抗壞血酸鎂*6 3.0 (1 2 )純水 5.0 (1 3 )乙醇 15.0 (14 )對氧基安息香酸乙酯 0.1 (1 5 )香料 剩餘量 * 1 曰本界面活性劑公司製 -46 200808332 (43) * 2 九善製藥公司製 *3 九善製藥公司製 * 4 純正藥品工業公司製 * 5 莎布公司製（森之—） * 6 日光化學公司製 (製法） A.成分（1 )〜（7 )混合後於7 〇它中加熱並溶解。 B .成分（8 )〜（1 2 )經混合溶解。 C ·成分（1 3 )〜（1 5 )經混合溶解。 D · A中加入B混合並冷卻後，加入C混合得到面膜。 實施例2-6使用皮膚時，可藉由包殼蛋白（involucrin )產生促進作用使得肌膚表皮的阻礙功能正常化，其爲肌 表皮的水分保持改善效果、肌膚粗糙防止效果等優良的面 膜0 (實施例2-7 :乳霜粉底） (成分） （％ ) (1 )失水山梨醇倍半油酸酯 3.0 (2 )自己乳化型單硬脂酸甘油酯 2.0 (3 )硬脂酸鋁 0.5 (4 )純地蠟 5.0 (5 )三十碳烷 1〇.〇 -47- 200808332 (44) (6 )流動石蠟 15.0 (7)凡士林 5.0 (8 )氧化鈦 8.0 (9 )滑石 4.0 (1 〇 )著色顔料 適量 (1 1 )對氧基安息香酸甲酯 0.05 (1 2 )丙二醇 5.0 (1 3 )金銀花萃取物* 1 1.0 (14)白樺萃取物*2 1.0 (1 5 )七葉樹萃取物* 3 1.0 (16)白樺樹液*4 10.0 (1 7 )純水 剩餘量 (1 8 )香料 適量 *1 長岡香料公司製 *2 九善製藥公司製 *3 香榮興業公司製 * 4 莎布公司製（森之零） (製法） A.成分（1 )〜（1 1 )加熱混合後保持70°C。 B·成分（12 )〜（17 )加熱混合後保持7(TC。 C . A中加入B後混合，均勻地進行乳化。 D ·將C冷卻後，加入成分（1 8 )並混合得到乳霜粉底。 -48- (45) (45)200808332 (實施例2-8 :液態粉底） (成分） （％ ) (1 )液狀含水羊毛脂 2.0 (2 )流動石蠟 5.0 (3 )硬脂酸 2.0 (4 )十六烷醇 1.0 (5 )自己乳化型單硬脂酸甘油酯 1.0 (6 )對甲氧基桂皮酸-2-乙基己酯 8.0 (7 ) 4-第三丁基-4’-甲氧基二苯甲醯基甲烷 2.0 (8 )棕櫚酸松香油* 1 0.2 (9 )對氧基安息香酸甲酯 0.1 (1 0 )甘油 5.0 (1 1 )三乙醇胺 1.0 (12) 羧基甲基纖維素 0.2 (13) Bentnite 0.5 (14) 白樺樹液*2 5.0 (15) 甘露糖醇*3 0.1 (1 6 )純水 剩餘量 (1 7 )氧化鈦 6.0 (1 8 )微粒子氧化鈦 2.0 (1 9 )微粒子氧化鋅 5.0 (20 )雲母 2.0 (2 1 )滑石 4.0 (22 )著色顔料 4.0 -49- 200808332 (46) 5.0 適量 (23 )白樺樹液* 4 (24 )蘆筍萃取物* 5 (2 5 )甘草萃取物* 6 (26 )香料 *1 日本羅修公司製 *2 九善製藥公司製 *3 Sigma公司製 * 4 莎布公司製（森之雫） *5 九善製藥公司製 *6 九善製藥公司製 (製法） A·成分（1 )〜（9 )經混合溶解。 B. A中加入成分（17 )〜（22 )後均勻混合，保持70°C。 C ·成分（1 0 )〜（1 6 )均勻地溶解，保持7 0 °C。 D . C中加入B後均勻地乳化。 E·將D冷卻後，加入成分（23 )〜（26 )混合，得到液態 粉底。 (實施例2-9 :防曬乳液） (成分） （％ ) (1 )聚氧伸烷基變性有機聚矽氧烷 1.0 (2 )二甲基聚矽氧烷 5.0 (3 )八甲基環四矽氧烷 20.0 -50- 200808332 (47) (4) 異壬酸異十三烷酯 (5) 對甲氧基桂皮酸-2·乙基己酯 (6 )對氧基安息香酸甲酯 0.1 (7) 聚矽氧處理微粒子氧化鈦 10.0 (8) 聚矽氧處理微粒子氧化鋅 10.0 (9) 聚苯乙烯粉末 3.0 (10 )三甲基甲矽烷氧矽酸 0.5 (1 1 )二丙二醇 3.0 (1 2 )乙醇 10.0 (1 3 )白樺樹液* 1 10.0 (14 )食鹽 0.2 (1 5 )純水 剩餘量 (16 )冷-胡蘿菠素* 2 〇.〇1 (1 7 )洋甘菊萃取物* 3 1.0 (1 8 )吾亦紅萃取物* 4 1.0 (1 9 )香料 適量 *1 莎布公司製（森之-) *2 Sigma公司製 *3 九善製藥公司製 *4 九善製藥公司製 (製法） A ·混合成分（1 )〜（1 0 )，保持7 0。(：。 B ·混合成分（1 1 )〜（1 5 )，保持7 0 °C。 -51 - 200808332 (48) C . A中添加B，均勻地進行乳化。 D.將C冷卻後，加入成分（1 6 )〜（1 9 )混合得到防曬乳 液。 實施例2-7〜9皆使用於皮膚時’可藉由包殼蛋白（ involucriii )產生促進作用使得肌膚表皮的阻礙功能正常 化，其爲肌膚表皮的水分保持改善效果、肌膚粗糙防止效 果優良的乳霜粉底、液態粉底及防曬乳液。 (實施例3 -1 :化妝水） (成分） (% ) (1 )甘油 3.0 (2 ) 1，3-丁二醇 2.0 (3 )丙二醇 4.0 (4 )油醇 0.1 (5)單月桂酸聚環氧乙烷（ 2 0 E · 0 ·)山梨醇酐 1 .5 (6 )聚環氧乙烷（20E.O·) 月桂醚 0.5 (7 )三十碳院 0.5 (8 )乙醇 20.0 (9 )對氧基安息香酸甲酯 0.1 (10 )乙酸dl- α -生育醇* 1 0.5 (1 1 )香料 適量 (1 2 )甘草酸二鉀* 2 0.5 (1 3 )迷迭香萃取物* 3 0.5 (14 ) dl-吡咯烷酮羧酸鈉液* 4 0.2 -52- 1 . 200808332 (49) 20.0 剩餘量 (1 5 )刺槐豆膠 (1 6 )薏仁萃取物* 5 (1 7 )白樺樹液* 6 (1 8 )純水 氺1 Eisai公司製 *2 九善製藥公司製 *3 九善製藥公司製 *4 味之素公司製 *5 九善製藥公司製 *6 莎布公司製（森之雫） (製法） A ·成分（1 )〜（1 1 )經混合溶解。 B ·成分（1 2 )〜（1 8 )經混合溶解。 C . A中加入B後均勻混合得到化妝水。 實施例3 -1具有水樣清爽的使用感，賦予肌膚濕潤感 ^ ，其爲可持續維持肌膚滑潤之化妝水 (實施例3-2 :乳霜） (% ) 20.0 (成分） (1 )三十碳院 (2 )蜜蠟 (3 )荷荷芭油 -53· (50) (50)200808332 (4)單硬脂酸甘油酯 2.0 (5 )單硬脂酸聚環氧乙烷（20E.O·)山梨醇酐 2.0 (6 )單硬脂酸山梨醇酐 2.0 (7 )植物醇* 1 0.5 (8 )堪地里蠟 1.0 (9)甘油 5.0 (1 〇 )對氧基安息香酸甲酯 0.1 (1 1 )百合萃取物* 2 1.0 (1 2 )人參萃取物* 3 1.0 (1 3 )銀杏萃取物* 4 1.0 (14)大棗萃取物*5 1.0 (1 5 )苦參萃取物* 6 1.0 (1 6 )水解米萃取物* 7 1.0 (1 7 )甘草萃取物* 8 1.0 (1 8 )當歸萃取物* 9 1.0 (1 9 )白樺樹液* 1 0 5.0 (2 0 )純水 剩餘量 (2 1 )香料 適量 氺1 Eisai公司製 *2 九善製藥公司製 *3 九善製藥公司製 * 4 常磐植物化學硏究所公司製 * 5 九善製藥公司製 *6 九善製藥公司製 -54 - 200808332 (51) 氺7 TECHNO-BULL公司製 *8 九善製藥公司製 * 9 日本粉末藥品公司製 * 10 莎布公司製（森之雫） (製法） 胃 A·成分（1)〜（10)加熱混合後保持70°c。 B·成分（1 1 )〜（20 )加熱混合後保持70°C。 C.A中加入B後進行乳化。 D ·將C冷卻後，加入成分（2 1 )並混合得到乳霜。 實施例3 -2具有滑潤且濃厚之使用感，賦予肌膚滑潤 與保濕感，其爲可持續維持肌膚滑潤的乳霜。 (實施例3-3 :潔面乳霜） (成分） （％ ) (1 )石鱲 10.0 (2 )蜜蠟 3.0 (3)凡士林 15.0 (4 )流動石蠟 41.0 (5 )失水山梨醇倍半油酸酯 4.2 (6 )單油酸聚環氧乙烷（20E.O.)山梨醇酐 0.8 (7 )氫化大豆磷脂質 0.5 (8)白芒花好油 （Meadowfoam) *1 1 .0 -55- 200808332 (52) (9 )對氧基安息香酸甲酯 0.1 (10 )溫椁種子萃取物* 2 1.0 (1 1 )石鹼草萃取物* 3 1.0 (12) 大花葵花萃取物* 4 1.0 (13) 白樺樹液*5 5.0 (1 4 )純水 剩餘量 (1 5 )香料 適量 * 1 日本界面活性劑工業公司製 *2 香榮興業公司製 *3 九善製藥公司製 *4 九善製藥公司製 *5 莎布公司製（森之學） (製法） A·成分（1 )〜（9 )加熱混合後保持7(TC。 B. 成分（10)〜（14)加熱混合後保持70°C。 C. A中加入B後進行乳化。 D. 將C冷卻後，加入成分（1 5 )並混合得到潔面乳霜。 實施例3-3具有輕薄滑潤的使用感，與彩妝的融合性 良好，潔面後之可持續維持肌膚滑潤的潔面乳霜。 (實施例3-4 :乳液） (成分） （％ ) -56- 200808332 (53) (1 )單硬脂酸山梨醇酐 0.3 (2 )單油酸聚環氧乙烷（20E.O·)山梨醇酐 0.1 (3 )親油型單硬脂酸甘油酯 0.2 (4 )硬脂酸 0.5 (5 )十六烷醇 0.5 (6 )三十碳烷 3.0 (7 )流動石蠟 4.0 (8 )植物性三十碳烷* 1 0.2 (9 )三-2-乙基己酸甘油酯 2.0 (10) 二甲基聚矽氧烷 1.0 (11) 氫化大豆磷脂質 0.1 (12) 煙酸dl-α-生育醇*2 0.05 (1 3 )對氧基安息香酸乙酯 0.1 (14 )羧基乙烯聚合物 0.1 (15 )氫氧化鈉 0.05 (1 6 )甘油 5 · 0 (17 ) 1，3-丁二醇 7.0 (1 8 )純水 剩餘量 (19) 白樺樹液*3 1〇.〇 (20) 芍藥萃取物*4 2.0 (21 )靈芝萃取物* 5 2.0 (2 2 )透明質酸鈉1 %水溶液* 6 5.0 (2 3 )乙醯基麩胺酸* 7 0.3 (24 )多孔質二氧化矽 3.0 -57- 200808332 (54) ( 25 ) 香料 適量 ( 26 ) 乙醇 5.0 氺 1 曰 光化 學 公 司 製 氺 2 Ei sai公司製 氺 3 莎 布公 司 製 ( 森之,） 氺 4 九 善製 藥 公 司 製 氺 5 九 善製 藥 公 司 製 氺 6 曰 光化 學 公 司 製 氺 7 協 和發 酵 公 司 製 (製法） Α·成分（14)〜（23)加熱混合後保持70°C。 B·成分（1 )〜（13 )加熱混合後保持70°C。 C . A中加入B後進行乳化。 D·將C冷卻後，加入成分（24 ) 、（ 25 ) 、（ 26 )並混合 得到乳液。 (實施例3 - 5 :乳液） (成分） （％ ) (1 )單硬脂酸山梨醇酐 0.3 (2 )單油酸聚環氧乙烷（20E.O·)山梨醇酐 0.1 (3 )親油型單硬脂酸甘油酯 0.2 (4 )硬脂酸 0.5 (5 )十六烷醇 0.5 -58- 200808332 (55) (6)三十碳院 3.0 (7 )流動石蠟 4.0 (8 )三-2-乙基己酸甘油酯 2.0 (9)二甲基聚矽氧烷 1.0 (1 〇 )氫化大豆磷脂質 〇·1 (1 1 )芸香苷* 1 0.2 (1 2 )對氧基安息香酸甲酯 0.1 (13)羧基乙烯聚合物 0.1 (1 4 )氫氧化鈉 〇.〇5 (1 5 )甘油 5.0 (16) 1，3-丁二醇 7.0 (17) 白樺樹液* 2 3.0 (1 8 )純水 剩餘量 (19)酵母萃取物*3 0.5 (2 0 )明日葉萃取物* 4 0.5 (2 1 )蛇麻草萃取物* 5 0.5 (22 )桃子萃取物* 6 0.5 (2 3 )多孔質二氧化矽 3.0 (24 )香料 適量 (2 5 )乙醇 5.0 * 1 Sigma公司製 *2 莎布公司製（森之雫） *3 B I O-DE L L 公司製 *4 九善製藥公司製 -59- 200808332 (56) *5 九善製藥公司製 *6 九善製藥公司製 (製法） A·成分（13 )〜（22 )加熱混合後保持70°C。 B·成分（1 )〜（12 )加熱混合後保持70°C。 C · A中加入B後進行乳化。 D·將C冷卻後，加入成分（23 ) 、（ 24 ) 、（ 25 )並混合 得到乳液。 實施例3-4及實施例3-5具有滑潤且柔和的使用感， 可賦予肌膚滑潤與適度保濕感，其爲可持續維持肌膚滑潤 的乳液。 (實施例3-6 ··面膜） (% ) 13.0 0.5 0.5 0.2 5.0 5.0 剩餘量 1.0 (成分） (1 )聚乙烯醇 (2 )無水矽酸 (3 )聚乙二醇 (4) 白芒花籽:油（Meadowfoam) *1 (5) 聚環氧丙烷（10P.O.)甲基葡萄苷 (6 )甘油 (7 )純水 (8 )茶萃取物* 2 -60- 200808332 (57) (6)精油玫瑰（RosarugosaThiinb)萃取物 *3 i . 〇 (1 〇 )無水咖啡因* 4 1.0 (11 )白樺樹液* 5 5.0 (12)磷酸-L -抗壞血酸鎂* 6 3.0 (1 3 )對氧基安息香酸乙酯 〇 . ! (Η)乙醇 20.0 (1 5 )香料 適量 * 1 日本界面活性劑工業公司製 *2 九善製藥公司製 *3 九善製藥公司製 * 4 純正藥品工業公司製 *5 莎布公司製（森之-) * 6 日光化學公司製 (製法） A·成分（1 )〜（7 )混合後於70°C中加熱並溶解。 B. 成分（8 )〜（1 2 )經混合溶解。 C. 成分（13 )〜（15 )經混合溶解。 D ·將B添加於A中，混合後冷卻，再混合c得到面膜。 實施例3 - 6爲具有適度清涼感與緊張感，經面膜後的 肌膚具有滑潤感與彈性感，其爲可持續維持肌膚滑潤的面 膜。 -61 - 200808332 (58) (實施例3-7 :乳霜粉底） (成分） （％ ) (1 )失水山梨醇倍半油酸酯 3.0 (2)自己乳化型單硬脂酸甘油酯 2.0 (3 )硬脂酸鋁 0.5 (4 )純地蠟 5.0 (5)三十碳院 10.0 (6 )流動石鱲 15.0 (7 )凡士林 5.0 (8 )氧化鈦 8.0 (9 )滑石 4.0 (1 〇 )著色顔料 適量 (1 1 )對氧基安息香酸甲酯 〇.〇5 (12 )丙二醇 5.0 (1 3 )金銀花萃取物* 1 1.0 (14)白樺萃取物*2 1.0 (15 )七葉樹萃取物* 3 1.0 (16)白樺樹液*4 10.0 (1 7 )純水 剩餘量 (1 8 )香料 適量 *1 長岡香料公司製 * 2 九善製藥公司製 *3 香榮興業公司製 *4 莎布公司製（森之韦） -62- (59) (59)200808332 (製法） A.成分（1)〜（11)加熱混合後保持70°C。 B·成分（12)〜（17)加熱混合後保持70°C。 C . A中加入B後進行乳化。 D.將C冷卻後，加入成分（1 8 )後混合得到乳霜粉底。 (實施例3 - 8 :液態粉底） (成分） （％ ) (1 )液狀含水羊毛脂 2.0 (2 )流動石蠟 5.0 (3 )硬脂酸 2.0 (4 )十六烷醇 1.0 (5 )自己乳化型單硬脂酸甘油酯 1.0 (6) 對甲氧基桂皮酸-2-乙基己酯 8.0 (7) 4-第三丁基- 4’-甲氧基二苯甲醯基甲烷 2.0 (8 )棕櫚酸松香油* 1 0.2 (9 )對氧基安息香酸甲酯 0.1 (1 〇 )甘油 5.0 (1 1 )三乙醇胺 1.0 (12 )羧基甲基纖維素 0.2 (13) Bentnite 0.5 (1 4 )白樺萃取液* 2 5.0 (1 5 )純水 剩餘量 (1 6 )氧化鈦 -63- 200808332 (60) (1 7 )微粒子氧化鈦 2 · 0 (1 8 )微粒子氧化鋅 5.0 (1 9 )雲母 2.0 4.0 4.0 0.1 5.0 適量 (2 0 )滑石 (2 1 )著色顔料 (22)甘露糖醇*3 (23 )白樺樹液* 4 (24 )蘆筍萃取物* (2 5 )甘草萃取物* (26 )香料 * 1 曰 本 羅 修 公 司 製 氺 2 九 善 製 藥 公 司 製 * 3 S i gm [a 公 司 製 氺 4 莎 布 公 司 製 ( 森之零） * 5 九 善 製 藥 公 司 製 氺 6 九 善 製 藥 公 司 製 (製法） A.成分（1 )〜（9 )經加熱溶解。 B · A中加入成分（1 6 )〜（2 1 )並均勻混合，保持7 0 °C。 C.成分（10)〜（15)經加熱溶解，保持70°C。 D . C中添加B後均勻地進行乳化。 E.將D冷卻後，加入成分（22 )〜（26 )並混合得到液態 粉底。 -64- 200808332 (61) 實施例3-7及實施例3-8具有濃厚且滑潤的使用感， 可得到均勻美感的上妝效果，且爲可持續維持肌膚滑潤的 乳霜粉底及液態粉底。 (實施例3-9 :防曬乳液） (成分） （％ ) (1 )聚氧伸烷基變性有機聚矽氧烷 1 ·〇 (2)二甲基聚矽氧烷 5·0 (3 )八甲基環四矽氧烷 20·〇 (4)異壬酸異十三烷酯 5·〇 (5 )對甲氧基桂皮酸-2-乙基己酯 5·〇 (6 )對氧基安息香酸甲酯 〇·1 (7)聚矽氧處理微粒子氧化鈦 1 〇 · 〇 (8 )聚矽氧處理微粒子氧化鋅 1〇·〇 (9) 聚苯乙烯粉末 3.0 (10) 三甲基甲矽烷氧矽酸 0.5 (1 1 )二丙二醇 3.0 (1 2 )乙醇 10.0 (13)白樺樹液*1 1〇.〇 (14 )食鹽 0.2 (1 5 )純水 剩餘量 (16 )冷-胡蘿菠素* 2 〇·〇1 (1 7 )洋甘菊萃取物* 3 1.0 -65 200808332 (62) (1 8 )吾亦紅萃取物* 4 1.0 (1 9 )香料 適量 *1 莎布公司製（森之雫） *2 Sigma公司製 *3 九善製藥公司製 *4 九善製藥公司製 (製法） A·成分（1 )〜（10 )加熱混合後保持70°C。 B·成分（1 1 )〜（15 )加熱混合後保持70°C。 C. A中加入B後進行乳化。 D. 將C冷卻後，加入成分（16)〜（19)並混合得到防曬 乳液。 實施例3-9具有無黏沾感且滑潤的使用感，可由紫外 線保護肌膚的同時亦可持續維持肌膚滑潤的防曬乳液。 (實施例4-1 :化妝水） (成分） （％ ) (1 )甘油 3.0 (2 ) 1，3-丁二醇 2.0 (3 )丙二醇 4.0 (4 )油醇 〇·1 (5 )單月桂酸聚環氧乙烷（20Ε.Ο·)山梨醇酐 -66 - 200808332 (63) (6 )聚環氧乙烷（20Ε·Ο·)月桂醚 0·5 (7 )三十碳烷 0.5 (8 )乙醇 20.0 (9 )對氧基安息香酸甲酯 0.1 (10 )乙酸dl- α -生育醇* 1 0.5 (1 1 )香料 適量 (12)甘草酸二鉀*2 0.5 (1 3 )迷迭香萃取物* 3 0.5 (14 ) dl-吡咯烷酮羧酸鈉* 4 0.2 (15 )刺槐豆膠 1.0 (1 6 )薏仁萃取物* 5 0.5 (17 )白樺樹液* 6 20.0 (1 8 )純水 剩餘量 * 1 Eisai公司製 *2 九善製藥公司製 *3 九善製藥公司製 * 4 味之素公司製 *5 九善製藥公司製 *6 莎布公司製（森之-) (製法） A ·成分（1 )〜（1 1 )經混合溶解。 B ·成分（1 2 )〜（1 8 )經混合溶解。 C . A中加入B並均勻混合後得到化妝水。 -67- 200808332 (64) 實施例4-1具有水樣清爽的使用感，可得到無肌膚粗 糙或肌膚黯淡等美麗肌膚之化妝水。 (實施例4-2 :乳霜） (成分） （％ ) (1 )三十碳烷 20.0 (2 )蜜蠟 (3 )荷荷芭油 5.0 (4)單硬脂酸甘油酯 2·0 (5 )單硬脂酸聚環氧乙烷（20Ε.Ο.)山梨醇酐 2.0 (6 )單硬脂酸山梨醇酐 2.0 (7 )植物醇* 1 0.5 (8 )堪地里蠟 1.0 (9 )甘油 5.0 (10)對氧基安息香酸甲酯 〇·1 (1 1 )百合萃取物* 2 1.0 (1 2 )人參萃取物* 3 1.0 (1 3 )銀杏萃取物* 4 1.0 (1 4 )大棗萃取物* 5 1.0 (1 5 )苦參萃取物* 6 1.0 (1 6 )水解米萃取物* 7 1.0 (1 7 )甘草萃取物* 8 1.0 (1 8 )當歸萃取物* 9 1.0 (1 9 )白樺樹液* 1 0 5.0 -68- 200808332 (65) (20 ) 純水 剩餘量 (21 ) 香料 適量 氺1 Eisai公司製 氺2 九善製藥公司製 氺3 九善製藥公司製 氺4 常磐植物化學硏究所公司製 氺5 九善製藥公司製 氺6 九善製藥公司製 *7 TECHNO-BULL 公司製 氺8 九善製藥公司製 氺9 日本粉末藥品公司製 *10 莎布公司製（森之零） (製法） Α·成分（1 )〜（10 )加熱混合後保持70°C。 B·成分（1 1 )〜（20 )加熱混合後保持70°C。 C . A中加入B後進行乳化。 D.將C冷卻後，加入成分（2 1 )並混合得到乳霜。 實施4 -2爲具有滑潤且濃厚的使用感，可得到無肌膚 粗糙或肌膚黯淡之美麗肌膚的乳霜。 (實施例4-3 :潔面乳霜） (成分） （％ ) -69- 200808332 (66) (1 )石蠟 (2 )蜜蠟 (3 )凡士林 (4 )流動石蠟 (5 )失水山梨醇倍半油酸酯 (6 )單油酸聚環氧乙烷（20E.O·)山梨醇酐 ^ ( 7 )氫化大豆磷脂質 (8)白芒花好油 （Meadowfoam) *1 (9 )對氧基安息香酸甲酯 (1 〇 )溫桴種子萃取物* 2 (1 1 )石鹼草萃取物* 3 (1 2 )大花葵花萃取物* 4 (1 3 )白樺樹液* 5 (1 4 )純水 (1 5 )香料 氺 1 曰 本 界 面 活 性 劑 公 司製 * 2 香 榮 興 業 公 司 製 氺 3 九 善 製 藥 公 司 製 氺 4 九 善 製 藥 公 司 製 氺 5 莎 布 公 司 製 ( 森 之 雫） (製法） A.成分（1 )〜（9 )加熱混合後保持7CTC。 B ·成分（1 0 )〜（1 4 )加熱混合後保持7 0 °C。 10.0 3.0 15.0 41.0 4.2 0.8 0.5 1 .0 0.1 1 .0 1.0 1.0 5.0 剩餘量 適量 -70- 200808332 (67) C. A中加入B後進行乳化。 D. 將C冷卻後，加入成分（1 5 )並混合得到潔面乳霜。 實施例4-3具有輕薄且滑潤的使用感，與彩妝的融合 性良好，具有優良的卸妝去污性，可得到無肌膚粗糙或肌 膚黯淡的美麗肌膚之潔面乳霜。 (實施例4-4 :乳液） (成分） （％ ) (1 )單硬脂酸山梨醇酐 0.3 (2 )單油酸聚環氧乙烷（20E.O.)山梨醇酐 0.1 (3 )親油型單硬脂酸甘油酯 0.2 (4 )硬脂酸 0.5 (5 )十六烷醇 0.5 (6)三十碳院 3.0 (7 )流動石蠟 4.0 (8 )植物性三十碳烷* 1 0.2 (9 )三-2-乙基己酸甘油酯 2.0 (10)二甲基聚矽氧烷 1·0 (1 1 )氫化大豆磷脂質 〇 · 1 (12)煙酸dl-α-生育醇*2 0.05 (1 3 )對氧基安息香酸乙酯 0 · 1 (14)羧基乙烯聚合物 0.1 (15 )氫氧化鈉 0.05 -71 - 200808332 (68) (1 6 )甘油 5.0 (17 ) 1，3·丁二醇 7·0 (1 8 )純水 剩餘量 (19) 白樺樹液*3 10.0 (20) 芍藥萃取物*4 2.0 (2 1 )靈芝萃取物* 5 2.0 (22 )透明質酸鈉1%水溶液* 6 5.0 (23 )乙醯基麩胺酸* 7 0.3 (24 )乙醇 5.0 (2 5 )多孔質二氧化矽 3.0 (26 )香料 適量 *1 日光化學公司製 氺2 Eisai公司製 *3 莎布公司製（森之雫） *4 九善製藥公司製 * 5 九善製藥公司製 氺6 日光化學公司製 * 7 協和發酵公司製 (製法） A. 成分（14)〜（23 )加熱混合後保持70°C。 B. 成分（1 )〜（13 )加熱混合後保持70°C。 C. A中加入B後進行乳化。 D. 將C冷卻後，加入成分（24 ) 、（ 25 ) 、（ 26 )並混合 -72- 200808332 (69) 得到乳液。 (實施例4-5 :乳液） (成分） （％ ) (1 )單硬脂酸山梨醇酐 0.3 (2 )單油酸聚環氧乙烷（20E.O·)山梨醇酐 0.1 (3 )親油型單硬脂酸甘油酯 0.2 (4 )硬脂酸 0.5 (5)十六院醇 0.5 (6 )三十碳烷 3.0 (7 )流動石蠟 4.0 (8 )三-2-乙基己酸甘油酯 2.0 (9)二甲基聚矽氧烷 1.0 (1 〇 )氫化大豆磷脂質 〇·1 (11 )芸香苷* 1 〇.2 (1 2 )對氧基安息香酸甲酯 0.1 (13)羧基乙烯聚合物 0.1 (14 )氫氧化鈉 0.05 (1 5 )甘油 5.0 (16 ) 1，3-丁二醇 7.0 (17)白樺樹液*2 3.0 (1 8 )純水 剩餘量 (1 9 )酵母萃取物* 3 0.5 (20 )明日葉萃取物* 4 0.5 -73- 200808332 (70) 0.5 5.0 3.0 適量 (2 1 )蛇麻草萃取物* 5 (22 )桃子萃取物* 6 (23 )乙醇 (24 )多孔質二氧化矽 (25 )香料 氺1 Sigma公司製 *2 莎布公司製（森之—） 氺3 BIO-DELL公司製 氺4 九善製藥公司製 *5 九善製藥公司製 氺6 九善製藥公司製 (製法） A·成分（13 )〜（22 )加熱混合後保持70°C。 B·成分（1)〜（12)加熱混合後保持70°C。 C · A中加入B後均勻地進行乳化。 D·將C冷卻後，加入成分（23 ) 、（ 24 ) 、（ 25 )並混合 得到乳液。 實施例4-4及實施例4-5具有滑潤且柔和的使用感， 可得到無肌膚粗糙或肌膚黯淡的美麗肌膚之乳液。 (% ) (實施例4-6 :面膜） (成分） -74- 200808332 (71) (1 )聚乙烯醇 13.0 (2 )無水矽酸 0.5 (3 )聚乙二醇 0.5 (4) 白芒花籽1 油 （Meadowfoam) *1 0.2 (5) 聚環氧丙烷（10P.Ο.)甲基葡萄苷 5.0 (6 )甘油 5.0 (7 )純水 剩餘量 (8 )茶萃取物* 2 1.0 (9 )精油玫瑰萃取物* 3 1.0 (1 0 )無水咖啡因* 4 1.0 (1 1 )白樺樹液* 5 5.0 (12)磷酸-L-抗壞血酸鎂*6 3.0 (1 3 )純水 10.0 (14 )對氧基安息香酸乙酯 0.1 (1 5 )乙醇 1 3 · 0 (1 6 )香料 適量 氺 1 曰 本 界 面 活 性 劑 工 業公司製 氺 2 九 善 製 藥 公 司 製 * 3 九 善 製 藥 公 司 製 氺 4 純 正 藥 品 工 業 公 司 製 氺 5 莎 布 公 司 製 ( 森 之 Ψ ) 氺 6 曰 光 化 學 公 司 製 (製法） -75 200808332 (72) Α·成分（1 )〜（7 )混合後於70°C中加熱並溶解。 B .成分（8 )〜（1 3 )經混合溶解。 C .成分（1 4 )〜（1 6 )經混合溶解。 D ·將B添加於A，混合後冷卻，再加入c並混合得到面膜 D ·將B添加於A，混合後冷卻，再加入c並混合得到面膜 〇 實施例4- 6具有適度清涼感與緊張感，經面膜後之肌 膚具有彈性’其爲可得到無肌膚粗糙或肌膚黯淡的美麗肌 膚之面膜。 (實施例4-7 :乳霜粉底） (成分） (〇/〇 ) (1 )失水山梨醇倍半油酸酯 3〇 (2 )自己乳化型單硬脂酸甘油酯 2.0 (3 )硬脂酸鋁 0.5 (4 )純地蠟 5.0 (5 )三十碳烷 10.0 (6)流動石蠘 15.0 (7 )凡士林 5.0 (8 )氧化鈦 8 . 〇 (9 )滑石 4.0 (1 〇 )著色顔料 適量 (11 )對氧基安息香酸甲酯 0.05 (12)丙二醇 5.0 -76- 200808332 (73) (1 3 )金銀花萃取物* 1 1.0 (14)白樺萃取物*2 1.0 (1 5 )七葉樹萃取物* 3 1.0 (16)白樺樹液*4 10.0 (1 7 )純水 剩餘量 (1 8 )香料 適量 * 1 長岡香料公司製 *2 九善製藥公司製 *3 香榮興業公司製 * 4 莎布公司製（森之雫） (製法） A·成分（1 )〜（1 1 )加熱混合後保持7(TC。 B·成分（12)〜（17)加熱混合後保持7(TC。 C . A中加入B後進行乳化。 D.將C冷卻後，加入成分（1 8 )並混合得到乳霜粉底。 (實施例4-8 :液態粉底） (成分） （％ ) (1 )液狀含水羊毛脂 2.0 (2 )流動石蠟 5.0 (3 )硬脂酸 2.0 (4 )十六烷醇 1·〇 (5 )自己乳化型單硬脂酸甘油酯 1.0 -77 200808332 (74) (6 )對甲氧基桂皮酸-2-乙基己酯 8.0 (7 ) 4-第三丁基-4’-甲氧基二苯甲醯基甲烷 2.0 (8 )棕櫚酸松香油* 1 0.2 (9 )對氧基安息香酸甲酯 0.1 (1 〇 )甘油 5.0 (1 1 )三乙醇胺 1.0 (12 )羧基甲基纖維素 0.2 (13) Bentnite 0.5 (14) 白樺萃取液*2 5.0 (1 5 )純水 剩餘量 (16) 甘露糖醇*3 0.1 (17) 白樺樹液*4 5.0 (1 8 )蘆筍萃取物* 5 0.5 (19)甘草萃取物*6 0.5 (20 )氧化鈦 6.0 (21 )微粒子氧化鈦 2.0 (22 )微粒子氧化鋅 5.0 (23 )雲母 2.0 (24 )滑石 4.0 (2 5 )著色顔料 4.0 (26 )香料 適量 *1 日本羅修公司製 *2 九善製藥公司製 *3 Sigma公司製 -78- 200808332 (75) *4 莎布公司製（森之—） *5 九善製藥公司製 *6 九善製藥公司製 (製法） A. 成分（1 )〜（9 )經混合溶解。 B. A中加入成分（20)〜（25)並均勻混合，保持70 °C。 C. 成分（1〇)〜（19)均勻地溶解，保持70 °C。 ^ D . C中加入B後進行乳化。 E.將D冷卻後，加入成分（26 )並混合得到液態粉底。 實施例4-7及4-8具有濃厚且滑潤的使用感，可得到 均勻且美麗的上妝效果，其爲可得到無肌膚粗糙或肌膚黯 淡的美麗肌膚之乳霜粉底、液態粉底。 (實施例4-9 :防曬乳液） (成分） （％ ) (1 )聚氧伸烷基變性有機聚矽氧烷 1.〇 (2)二甲基聚矽氧烷 5.0 (3 )八甲基環四矽氧烷 20.0 (4)異壬酸異十三烷酯 5.0 (5 )對甲氧基桂皮酸-2-乙基己酯 5.0 (6 )對氧基安息香酸甲酯 0.1 (7 )聚矽氧處理微粒子氧化鈦 10.0 -79- 200808332 (76) (8 )聚矽氧處理微粒子氧化鋅 10.0 (9) 聚苯乙嫌粉末 3.0 (10) 三甲基甲矽烷氧矽酸 0.5 (1 1 )二丙二醇 3.0 (1 2 )乙醇 10.0 (13)白樺樹液*1 10.0 (14 )食鹽 0·2 (1 5 )純水 剩餘量 (16 ) /3 -胡蘿菠素 * 2 0.01 (1 7 )洋甘菊萃取物* 3 1.0 (1 8 )吾亦紅萃取物* 4 1.0 (1 9 )香料 適量 *1 莎布公司製（森之-) *2 Sigma公司製 *3 九善製藥公司製 *4 九善製藥公司製 (製法） A.混合成分（1 )〜（1 0 )，保持7 (ΓC。 B·混合成分（11)〜（15)，保持7(TC。 C . A中添加B後均勻地進行乳化。 D·將C冷卻後，加入成分（1 6 )〜（1 9 )並混合得到防曬 乳液。 -80- 200808332 (77) 實施例4-9具有無黏沾感且滑潤的使用感，可由紫外 線保護肌膚的同時，可得到無肌膚粗糙或肌膚黯淡的美麗 肌膚之防曬乳液。 【圖式簡單說明】 〔圖1〕表皮細胞角化促進作用試驗結果；添加白樺 ^ 樹液1 〇%時的細胞形態（實施例1) 〔圖2〕表皮細胞角化促進作用試驗結果；無添加時 的細胞形態（比較例1 ) 〔圖3〕表皮細胞角化促進作用試驗結果；添加 1 .5mM氯化鈣時的細胞形態（比較例2 ) 〔圖4〕表皮細胞角化促進作用試驗；藉由MTTas say 之活細胞數比較 〔圖5〕細胞增殖促進試驗結果 -81 -(1) Polyoxyalkylene-denatured organic polyoxyalkylene i Q (2) dimethyl polyoxyalkylene 5. 0 (3) octamethylcyclotetraoxane 2 〇 4 (4) isophthalic acid isotrifate 5. 0 (5) p-methoxycinnamic acid-2-ethylhexyl ester 50 (6 ) p-oxybenzoic acid methyl ester 〇 .  ;t (7) polyfluorene treatment of fine titanium oxide 1〇. 〇 (8) polyfluorene treatment of micronized zinc oxide 丨〇.  〇 (9) polystyrene powder 3. 0 (1 0 ) trimethylmethoxine hydroxamic acid 0. 5 (1 1 ) dipropylene glycol 3. 0 -36- (33) (33)200808332 (1 2 ) Ethanol 10. 0 (1 3 ) Birch sap* i 10. 0 (14) salt 0. 2 (1 5 ) Pure water Remaining amount (16) 々-Carotene * 2 0. 0 1 (1 7 ) Chamomile Extract * 3 1. 0 (1 8 ) I also red extract * 4 1 . 0 (1 9 ) The right amount of spices *1 Shabu company system (Sen Zhi Bang) *2 Sigma company system *3 Jiushan Pharmaceutical Co., Ltd. 氺4 Jiushan Pharmaceutical Co., Ltd. (manufacturing method) Α·mixing ingredients (1)~(10 ), keep at 70 ° C. B. Mix the components (1 1 ) to (15) and keep at 70 °C. After adding B to C · A, the emulsion was uniformly performed. After D·cooling, ingredients (1 6 ) to (1 9 ) are added to C to obtain a sunscreen lotion. Example 1 -7~9 can be used in the skin, and can be promoted by profilaggrin and/or filaggrin, so that the skin itself produces a natural moisturizing factor, so A cream foundation, liquid foundation and sunscreen lotion that maintains smooth skin. -37- (34) (34) 200808332 (Example 2-1: lotion) (ingredient) (%) (1) glycerin 3. 0 (2) 1,3-butanediol 2. 0 (3) propylene glycol 4. 0 (4) oleyl alcohol 0. 1 (5) Single lauric acid polyethylene oxide (20E. 0. ) sorbitol 1. 5 (6) Polyethylene oxide (20E. 0. ) lauryl ether 0. 5 (7) triacontane 0. 5 (8) Ethanol 2 0. 0 (9) p-oxybenzoic acid methyl ester 0. 1 (10) acetic acid dl-α-tocopherol 氺1 0. 5 (1 1 ) Dipotassium glycyrrhizinate* 2 0. 5 (12) Rosemary extract *3 0. 5 (13) dl-pyrrolidone carboxylic acid sodium solution* 4 0. 2 (1 4 ) Locust bean gum 1. 0 (1 5 ) Coix seed extract * 5 0. 5 (16) Birch sap* 6 20. 0 (1 7 ) Remaining amount of pure water * 1 Eisai company *2 Manufactured by Jiu-Gang Pharmaceutical Co., Ltd. *3 manufactured by Jiu-Sen Pharmaceutical Co., Ltd. *4 Ajinomoto Co., Ltd.*5 Manufactured by Jiu-Zhong Pharmaceutical Co., Ltd. -38- 200808332 (35) * 6 Saab company system (Mori no.) (Production method) A·Components (1) to (10) are mixed and dissolved. B · The components (1 1 ) to (1 7 ) are dissolved by mixing. C.  After adding B to A, it is uniformly mixed to obtain a lotion. (Example 2 - 2: cream) (ingredient) (%) (1) triacontane 20. 0 (2) beeswax 5. 0 (3) jojoba oil 5. 0 (4) glyceryl monostearate 2. 0 (5) monostearic acid polyethylene oxide (20E. 0·) sorbitol 2. 0 (6) sorbitan monostearate 2. 0 (7) plant alcohol * 1 0. 5 (8) Candid wax 1. 0 (9) glycerol 5. 0 (10 ) p-oxybenzoic acid methyl ester 0. 1 (1 1 ) Lily Extract * 2 1. 0 (1 2 ) ginseng extract * 3 1. 0 (1 3 ) Ginkgo Biloba Extract * 4 1. 0 (14) jujube extract *5 1. 0 (1 5 ) Sophora flavescens extract * 6 1. 0 -39- 200808332 (36) 1. 0 1. 0 5. 0 Residual amount (1 6 ) Hydrolyzed rice extract * 7 (1 7 ) Licorice extract * 8 (1 8 ) Angelica extract * 9 (1 9 ) Birch sap * 1 0 (2 0 ) Pure water (2 1 )Spices* 1 made by Eisai Co., Ltd.*2 Manufactured by Jiushan Pharmaceutical Co., Ltd. * *3 Manufactured by Jiushan Pharmaceutical Co., Ltd. * 4 磐 磐 磐 制药 制药 * 九 九 九 九 九 九 制药 TECH TECH BULL company system*8 manufactured by Jiuzhan Pharmaceutical Co., Ltd.*9 manufactured by Japan Powder Pharmaceutical Co., Ltd.*10 Saab Co., Ltd. (Sengzhi-) '(Processing method) A·Components (1) to (10) are kept at 70 °C after heating and mixing. B·Components (1 1 ) to (20) were kept at 70 ° C after heating and mixing. C. Emulsification was carried out after adding B to A. D · After cooling C, the component (2 1 ) is added and mixed to obtain a cream. When both of Example 2-1 and Example 2-2 are used on the skin, the inhibition function of the epidermis of the skin can be normalized by the promotion of the envelope protein (invoUcrin), which is the epidermis of the skin. A lotion and cream with excellent moisture retention and skin roughness prevention. (Example 2-3: Facial cleansing cream) (ingredient) (%) (1) Paraffin wax 10. 0 (2) beeswax 3. 0 (3) Vaseline 15. 0 (4) Flowing paraffin 41. 0 (5) sorbitan sesquioleate 4. 2 (6) monooleic acid polyethylene oxide (20 Ε · 0 ·) sorbitol 0. 8 (7) Hydrogenated soybean phospholipids 0. 5 (8) White Mango Oil (Meadowfoam) *1 1. 0 (9) Methyl benzobenzoate 0. 1 (1 〇 ) Wenyu Seed Extract* 2 1. 0 (1 1 ) Equilibrium Extract * 3 1. 0 (12) Large flower sunflower extract *4 1. 0 (1 3 ) Birch sap * 5 5. 0 (1 4 ) Remaining amount of pure water (1 5 ) Proper amount of fragrance * 1 Made by Japan Surfactant Co., Ltd. *2 manufactured by Fraser Industrial Co., Ltd. *3 Manufactured by Jiuzhan Pharmaceutical Co., Ltd. -41 - 200808332 (38) * 4 Jiushan Pharmaceutical Co., Ltd. System * 5 Saab company system (Senzhi -) (manufacturing method) A·Component (1) ~ (9) After heating and mixing, keep 7 (KC. B. Composition (10) ~ (14) After heating and mixing, keep 70. :. C.  After adding B to A, it was mixed and emulsified uniformly. D.  After cooling C, the ingredients (15) were added and mixed to obtain a cleansing cream. When the skin is applied to the skin of Example 2-3, it can promote the barrier function of the epidermis by promoting the action of the inv〇iucrin, which is an excellent effect of improving the moisture retention of the skin epidermis and preventing the skin from being rough. Cleansing cream. (Example 2-4: Emulsion) (ingredient) (%) (1) Sorbitan monostearate 0. 3 (2) monooleic acid polyethylene oxide (20E. 0·) Sorbitol 〇·1 (3) lipophilic glyceryl monostearate 〇·2 (4) bismuth stearate·5 (5) cetyl hydrazine·5 (6) triacontane 3. 0 (7) Mobile sarcophagus 4. 0 (8) vegetal triacontan * 1 〇. 2 •42- (39) (39)200808332 (9) Tris-2-ethylhexanoic acid glyceride 2·0 (10) Dimethyl polyoxane 1. 0 (11) Hydrogenated soybean phospholipid 〇·1 (12) Niacin dl-α-tocopherol*2 0. 05 (1 3 ) p-oxybenzoic acid ethyl ester 0. 1 (14) Carboxyvinyl polymer 0. 1 (1 5 ) sodium hydroxide 0. 05 (1 6 ) glycerol 5. 0 (17) 1,3-butanediol 7. 0 (1 8 ) ethanol 5. 0 (19) Birch sap*3 10. 0 (20) Peony Extract * 4 2. 0 (21) Ganoderma lucidum extract *5 2. 0 (2 2 ) sodium hyaluronate 1% aqueous solution * 6 5. 0 (23) Ethyl glutamic acid * 7 0. 3 (2 4 ) pure water remaining amount (25) porous ceria 3. 0 (26) Proper amount of spices* 1 manufactured by Nikko Chemical Co., Ltd. *2 manufactured by Eisai Co., Ltd. * 3 Shabu Co., Ltd. (Mori Zero) * 4 manufactured by Jiu-Sen Pharmaceutical Co., Ltd. * 5 manufactured by Jiu-Zhong Pharmaceutical Co., Ltd. *6 manufactured by Nikko Chemical Co., Ltd. -43 - 200808332 (40) * 7 Concord Fermentation Company (Manufacturing Method) A. The components (14) to (24) were heated and mixed, and kept at 70 °C. B·Components (1) to (13) were kept at 70 ° C after heating and mixing. C. After adding B to A, it was mixed and emulsified uniformly. D. After cooling C, the components (25) and (26) are added to obtain an emulsion. (Example 2-5: Emulsion) (ingredient) (%) (1) Sorbitan monostearate 0. 3 (2) monooleic acid polyethylene oxide (20E. 0·) sorbitol 0. 1 (3) lipophilic glyceryl monostearate 0. 2 (4) stearic acid 0. 5 (5) cetyl alcohol 0. 5 (6) triacontane 3. 0 (7) mobile paraffin 4. 0 (8) tris-2-ethylhexanoic acid glyceride 2. 0 (9) dimethyl polyoxane 1. 0 (1 〇 ) hydrogenated soybean phospholipid 〇·1 (1 1 ) rutin* 1 0. 2 (12) p-oxybenzoic acid methyl ester 0. 1 (1 3 )carboxyvinyl polymer 0. 1 (1 4 ) sodium hydroxide 0. 05 -44 - 200808332 (41) (1 5 ) Glycerin 5. 0 (16) 1,3-butanediol 7. 0 (17) Birch sap*2 3. 0 (1 8 ) pure water remaining amount (1 9 ) ethanol 5. 0 (20) Yeast Extract* 3 0. 5 (2 1 ) tomorrow leaf extract * 4 0. 5 (22) Hop Extracts* 5 0. 5 (23) Peach Extract* 6 0. 5 (24) Porous ceria 3. 0 (2 5 ) Proper amount of spices * 1 Sigma company *2 Shabu company system (Sengzhi 雫) 氺3 BIO-DELL company system * 4 Jiushan Pharmaceutical Co., Ltd. *5 Jiushan Pharmaceutical Co., Ltd. *6 Jiushan Pharmaceutical Co., Ltd. System (manufacturing method) A.  The components (13) to (23) were kept at 70 ° C after heating and mixing. B.  The components (1) to (12) were kept at 70 ° C after heating and mixing. C.  After adding B to A, it was mixed and emulsified uniformly. D. After cooling C, the components (24) and (25) are added to obtain an emulsion. -45- (42) (42) 200808332 When both of Example 2-4 and Example 2-5 are used on the skin, the barrier function of the epidermis of the skin can be normalized by the promotion of the encapsulation protein (invoUcrin). An emulsion that improves the moisture retention of the skin's epidermis and provides excellent skin roughness. (Example 2-6: Mask) (Component) (%) (1) Polyvinyl alcohol 15. 0 (2) Anhydrous citric acid 0. 5 (3) Polyethylene glycol 0. 5 (4) White Mango Oil (Meadowfoam) *1 0. 2 (5) Polypropylene oxide (1 0P. Hey. )methylglucoside 5. 0 (6) glycerol 5. 0 (7) pure water remaining amount (7) tea extract *2 1. 0 (8) Essential Oil Rose (R〇SarugosaThunb) Extract *3 1. 0 (9) Anhydrous caffeine* 4 1. 0 (1 0 ) Birch sap* 5 5 · 0 (11) Phosphate-L-ascorbate*6 3. 0 (1 2 ) pure water 5. 0 (1 3 ) ethanol 15. 0 (14) p-oxybenzoic acid ethyl ester 0. 1 (1 5 ) Residual amount of fragrance * 1 界面 界面 界面 界面 46 46 46 46 46 46 46 46 46 46 46 46 46 46 46 46 46 46 46 46 46 46 46 46 46 46 46 46 46 46 46 九 九 九 九 九 九 九 九 九 九 九 九 九 九 九(森之—) * 6 Nikko Chemical Co., Ltd. (Manufacturing Method) A. The ingredients (1) to (7) are mixed and heated and dissolved in 7 Torr. B. The components (8) to (1 2) are dissolved by mixing. C · The components (1 3 ) to (1 5 ) are dissolved by mixing. D·A was added with B and cooled, and then added with C to obtain a mask. When the skin of Example 2-6 is used, it is possible to normalize the barrier function of the epidermis by promoting the action of involucrin, which is an excellent mask for improving the moisture retention of the muscle epidermis and the skin roughness prevention effect ( Example 2-7: Cream foundation) (ingredient) (%) (1) sorbitan sesquioleate 3. 0 (2) Self-emulsified glyceryl monostearate 2. 0 (3) aluminum stearate 0. 5 (4) pure ground wax 5. 0 (5) triacontane 1〇. 〇 -47- 200808332 (44) (6) Mobile paraffin 15. 0 (7) Vaseline 5. 0 (8) titanium oxide 8. 0 (9) talc 4. 0 (1 〇 ) coloring pigment appropriate amount (1 1 ) p-oxybenzoic acid methyl ester 0. 05 (1 2 ) Propylene glycol 5. 0 (1 3 ) Honeysuckle Extract * 1 1. 0 (14) White birch extract *2 1. 0 (1 5 ) Horse chestnut extract * 3 1. 0 (16) Birch sap*4 10. 0 (1 7 ) Remaining amount of pure water (1 8 ) Proper amount of spices *1 manufactured by Nagaoka Spice Co., Ltd. *2 manufactured by Jiu-Sen Pharmaceutical Co., Ltd. *3 manufactured by Fraser Corporation * 4 Saab Co., Ltd. (Mori Zero) (Method) A . The components (1) to (1 1 ) were kept at 70 ° C after heating and mixing. B·Component (12)~(17) After heating and mixing, keep 7 (TC.C.  After adding B to A, it was mixed and emulsified uniformly. D. After cooling C, the ingredients (18) are added and mixed to obtain a cream foundation. -48- (45) (45) 200808332 (Example 2-8: Liquid Foundation) (ingredient) (%) (1) Liquid aqueous lanolin 2. 0 (2) mobile paraffin 5. 0 (3) stearic acid 2. 0 (4) cetyl alcohol 1. 0 (5) Self-emulsified glyceryl monostearate 1. 0 (6 ) p-methoxycinnamic acid-2-ethylhexyl ester 8. 0 (7) 4-Tert-butyl-4'-methoxybenzhydrylmethane 2. 0 (8) palmitic rosin oil * 1 0. 2 (9) p-oxybenzoic acid methyl ester 0. 1 (1 0 ) glycerol 5. 0 (1 1 ) triethanolamine 1. 0 (12) carboxymethyl cellulose 0. 2 (13) Bentnite 0. 5 (14) Birch sap*2 5. 0 (15) Mannitol*3 0. 1 (1 6 ) pure water remaining amount (1 7 ) titanium oxide 6. 0 (1 8 ) microparticle titanium oxide 2. 0 (1 9 ) micronized zinc oxide 5. 0 (20) mica 2. 0 (2 1 ) talc 4. 0 (22) coloring pigment 4. 0 -49- 200808332 (46) 5. 0 Appropriate amount (23) Birch sap* 4 (24) Asparagus extract * 5 (2 5 ) Licorice extract * 6 (26 ) Spice *1 Made by Japan Rosho Corporation *2 Made by Jiu-Zhong Pharmaceutical Co., Ltd. *3 Sigma company* 4 Saab company system (Mori no Kiseki) *5 Nine-good pharmaceutical company system *6 Nine-good pharmaceutical company system (manufacturing method) A·Components (1) to (9) are mixed and dissolved. B.  After adding components (17) to (22) to A, they were uniformly mixed and kept at 70 °C. C. The components (10) to (16) were uniformly dissolved and kept at 70 °C. D.  After adding B to C, it was uniformly emulsified. E. After cooling D, the components (23) to (26) are added and mixed to obtain a liquid foundation. (Example 2-9: sunscreen lotion) (ingredient) (%) (1) polyoxyalkylene-denatured organic polyoxyalkylene 1. 0 (2) dimethyl polyoxane 5. 0 (3) octamethylcyclotetraoxane 20. 0 -50- 200808332 (47) (4) Isotridecyl isononanoate (5) p-methoxycinnamic acid-2-ethylhexyl ester (6) p-oxybenzoic acid methyl ester 0. 1 (7) Polyfluorene treatment of fine particles of titanium oxide 10. 0 (8) Polyfluorene treatment of micronized zinc oxide 10. 0 (9) Polystyrene powder 3. 0 (10) trimethylmethoxine hydroxamic acid 0. 5 (1 1 ) dipropylene glycol 3. 0 (1 2 ) Ethanol 10. 0 (1 3 ) Birch sap* 1 10. 0 (14) salt 0. 2 (1 5 ) pure water remaining amount (16) cold - carotene * 2 〇. 〇1 (1 7 ) Chamomile Extract * 3 1. 0 (1 8 ) My Red Extract* 4 1. 0 (1 9 ) The right amount of spices *1 Shabu company system (Mori no -) *2 Sigma company *3 Jiushan Pharmaceutical Co., Ltd. *4 Jiushan Pharmaceutical Co., Ltd. (manufacturing method) A · Mixed ingredients (1) ~ (1 0), keep 7 0. (: B. Mixing ingredients (1 1 ) to (1 5 ), maintaining 70 ° C. -51 - 200808332 (48) C .  B is added to A, and emulsification is performed uniformly. D. After cooling C, the ingredients (16) to (1 9) are added and mixed to obtain a sunscreen emulsion. When all of the examples 2-7 to 9 are used on the skin, the effect of the encapsulation protein (involucriii) can be normalized to normalize the barrier function of the epidermis of the skin, which is an effect of improving the moisture retention of the skin epidermis and preventing the skin from being rough. Cream foundation, liquid foundation and sunscreen lotion. (Example 3-1: lotion) (ingredient) (%) (1) glycerin 3. 0 (2) 1,3-butanediol 2. 0 (3) propylene glycol 4. 0 (4) oleyl alcohol 0. 1 (5) Single lauric acid polyethylene oxide (20 E · 0 ·) sorbitol 1 . 5 (6) Polyethylene oxide (20E. O·) Lauryl ether 0. 5 (7) Thirty Carbon Court 0. 5 (8) Ethanol 20. 0 (9) p-oxybenzoic acid methyl ester 0. 1 (10) acetic acid dl-α-tocopherol* 1 0. 5 (1 1 ) Spice Appropriate amount (1 2 ) Dipotassium glycyrrhizinate* 2 0. 5 (1 3 ) Rosemary Extract * 3 0. 5 (14) dl-pyrrolidone carboxylic acid sodium solution* 4 0. 2 -52- 1 .  200808332 (49) 20. 0 Remaining amount (1 5 ) Locust bean gum (1 6 ) Coix seed extract * 5 (1 7 ) Birch sap * 6 (1 8 ) Pure water 氺 1 Eisai company *2 九善制药股份有限公司*3 九善制药Company*4 Ajinomoto Co., Ltd.*5 Manufactured by Jiushan Pharmaceutical Co., Ltd.*6 Saab Co., Ltd. (Sengzhi) (Production Method) A • Components (1) to (1 1 ) are mixed and dissolved. B · The components (1 2 ) to (1 8 ) are dissolved by mixing. C.  After adding B to A, it is uniformly mixed to obtain a lotion. Example 3-1 has a refreshing feeling of use in water, and gives a moist feeling to the skin. It is a lotion which can maintain the smoothness of the skin (Example 3-2: Cream) (%) 20. 0 (ingredient) (1) thirty carbon yard (2) beeswax (3) jojoba oil -53· (50) (50)200808332 (4) glyceryl monostearate 2. 0 (5) monostearic acid polyethylene oxide (20E. O·) sorbitol 2. 0 (6) sorbitan monostearate 2. 0 (7) plant alcohol * 1 0. 5 (8) Candid wax 1. 0 (9) glycerin 5. 0 (1 〇 ) p-oxybenzoic acid methyl ester 0. 1 (1 1 ) Lily Extract * 2 1. 0 (1 2 ) ginseng extract * 3 1. 0 (1 3 ) Ginkgo Biloba Extract * 4 1. 0 (14) jujube extract *5 1. 0 (1 5 ) Sophora flavescens extract * 6 1. 0 (1 6 ) Hydrolyzed Rice Extract* 7 1. 0 (1 7 ) Licorice Extract * 8 1. 0 (1 8 ) Angelica extract * 9 1. 0 (1 9 ) Birch sap * 1 0 5. 0 (2 0 ) Remaining amount of pure water (2 1 ) Proper amount of 氺 1 Eisai company *2 Manufactured by Jiu-Gang Pharmaceutical Co., Ltd. *3 Manufactured by Jiu-Shan Pharmaceutical Co., Ltd. * 4 磐 磐 磐 制药 制药 5 5 5 *6 九善制药股份有限公司-54 - 200808332 (51) 氺7 TECHNO-BULL company*8 九善制药公司制制* 9 Japan Powder Pharmaceutical Co., Ltd.* 10 Saab company system (Mori no 雫) (Method) Stomach A·Components (1) to (10) were kept at 70 ° C after heating and mixing. B·Components (1 1 ) to (20) were kept at 70 ° C after heating and mixing. C. Emulsification was carried out after adding B to A. D. After cooling C, the component (2 1 ) is added and mixed to obtain a cream. Example 3-2 has a smooth and strong feeling of use, which gives the skin a smooth and moisturizing feeling, and is a cream which can maintain the smoothness of the skin. (Example 3-3: Facial cleansing cream) (ingredient) (%) (1) Dendrobium 10. 0 (2) beeswax 3. 0 (3) Vaseline 15. 0 (4) Flowing paraffin 41. 0 (5) sorbitan sesquioleate 4. 2 (6) monooleic acid polyethylene oxide (20E. O. ) sorbitol 0. 8 (7) hydrogenated soybean phospholipids 0. 5 (8) White Mango Oil (Meadowfoam) *1 1 . 0 -55- 200808332 (52) (9) Methyl benzobenzoate 0. 1 (10) Wenjing Seed Extract* 2 1. 0 (1 1 ) Equilibrium Extract * 3 1. 0 (12) Large flower sunflower extract* 4 1. 0 (13) Birch sap*5 5. 0 (1 4 ) Remaining amount of pure water (1 5 ) Proper amount of fragrance * 1 Made by Japan Interacting Agent Industrial Co., Ltd. *2 manufactured by Fraser Industrial Co., Ltd. *3 manufactured by Jiushan Pharmaceutical Co., Ltd. *4 manufactured by Jiushan Pharmaceutical Co., Ltd. *5 Saab Company System (School of Mori) (Manufacturing Method) A·Component (1)~(9) After heating and mixing, keep 7 (TC. B.  The components (10) to (14) were heated and mixed, and kept at 70 °C. C.  Emulsification was carried out after adding B to A. D.  After cooling C, the ingredients (15) were added and mixed to obtain a cleansing cream. Example 3-3 has a light and smooth feeling of use, and is excellent in blending with makeup, and a cleansing cream which can maintain a smooth skin after cleansing. (Example 3-4: Emulsion) (ingredient) (%) -56- 200808332 (53) (1) Sorbitan monostearate 0. 3 (2) monooleic acid polyethylene oxide (20E. O·) sorbitol 0. 1 (3) lipophilic glyceryl monostearate 0. 2 (4) stearic acid 0. 5 (5) cetyl alcohol 0. 5 (6) triacontane 3. 0 (7) mobile paraffin 4. 0 (8) vegetal triacontan* 1 0. 2 (9) Tris-2-ethylhexanoic acid glyceride 2. 0 (10) dimethyl polyoxane 1. 0 (11) Hydrogenated soybean phospholipids 0. 1 (12) Niacin dl-α-tocopherol*2 0. 05 (1 3 ) p-oxybenzoic acid ethyl ester 0. 1 (14) carboxyvinyl polymer 0. 1 (15) sodium hydroxide 0. 05 (1 6 ) glycerol 5 · 0 (17 ) 1,3-butanediol 7. 0 (1 8 ) Pure water Remaining amount (19) Birch sap *3 1〇. 〇 (20) Peony Extract *4 2. 0 (21) Ganoderma lucidum extract * 5 2. 0 (2 2 ) sodium hyaluronate 1% aqueous solution * 6 5. 0 (2 3 ) Ethyl glutamic acid * 7 0. 3 (24) Porous ceria 3. 0 -57- 200808332 (54) ( 25 ) Proper amount of flavor ( 26 ) Ethanol 5. 0 氺1 曰 化学 化学 E E E E E E E E E E E E E E E E E E E 莎 莎 九 九 九 九 九 九 九 九 九 九 九 九 九 九 九 九 九 九 九 九 九 九 九 九 九 九 九Company system (manufacturing method) Α·Components (14) to (23) are heated and mixed and kept at 70 °C. B·Components (1) to (13) were kept at 70 ° C after heating and mixing. C.  Emulsification was carried out after adding B to A. D. After cooling C, components (24), (25), and (26) are added and mixed to obtain an emulsion. (Examples 3 - 5: Emulsion) (ingredients) (%) (1) Sorbitan monostearate 0. 3 (2) monooleic acid polyethylene oxide (20E. O·) sorbitol 0. 1 (3) lipophilic glyceryl monostearate 0. 2 (4) stearic acid 0. 5 (5) cetyl alcohol 0. 5 -58- 200808332 (55) (6) 30-carbon institute 3. 0 (7) mobile paraffin 4. 0 (8) tris-2-ethylhexanoic acid glyceride 2. 0 (9) dimethyl polyoxane 1. 0 (1 〇 ) hydrogenated soybean phospholipid 〇·1 (1 1 ) rutin* 1 0. 2 (1 2 )-p-oxybenzoic acid methyl ester 0. 1 (13) Carboxyvinyl polymer 0. 1 (1 4 ) sodium hydroxide 〇. 〇5 (1 5 ) glycerol 5. 0 (16) 1,3-butanediol 7. 0 (17) Birch sap* 2 3. 0 (1 8 ) pure water remaining amount (19) yeast extract *3 0. 5 (2 0 ) tomorrow leaf extract * 4 0. 5 (2 1 ) Hop Extract* 5 0. 5 (22) Peach Extract* 6 0. 5 (2 3 ) Porous ceria 3. 0 (24) Spices (2 5 ) Ethanol 5. 0 * 1 Sigma company*2 Shabu company system (Mori no 雫) *3 BI O-DE LL company system*4 九善制药股份有限公司-59- 200808332 (56) *5 九善制药股份有限公司*6 九Manufactured by Good Pharmaceuticals Co., Ltd. (Preparation method) A·Components (13) to (22) are kept at 70 ° C after heating and mixing. B·Components (1) to (12) were kept at 70 ° C after heating and mixing. Emulsify after adding B to C · A. D. After cooling C, components (23), (24), and (25) are added and mixed to obtain an emulsion. Examples 3-4 and Examples 3-5 have a smooth and soft feeling of use, which imparts a smooth and moderate moisturizing sensation to the skin, and is an emulsion which can maintain a smooth skin. (Example 3-6 ·· Mask) (%) 13. 0 0. 5 0. 5 0. 2 5. 0 5. 0 remaining amount 1. 0 (ingredient) (1) polyvinyl alcohol (2) anhydrous citric acid (3) polyethylene glycol (4) white mango seed: oil (Meadowfoam) *1 (5) polypropylene oxide (10P. O. )methyl glucoside (6) glycerol (7) pure water (8) tea extract * 2 -60- 200808332 (57) (6) essential oil rose (Rosarugosa Thiinb) extract *3 i .  〇 (1 〇) Anhydrous caffeine* 4 1. 0 (11) Birch sap* 5 5. 0 (12) Phosphoric acid-L-magnesium ascorbate* 6 3. 0 (1 3 ) p - oxybenzoic acid ethyl ester .  ! (Η) Ethanol 20. 0 (1 5 ) Proper amount of fragrance * 1 Made by Japan Interacting Agent Industry Co., Ltd. *2 manufactured by Jiu-Sen Pharmaceutical Co., Ltd. *3 manufactured by Jiu-Shan Pharmaceutical Co., Ltd. * 4 Pure Pharmaceutical Industry Co., Ltd. *5 Saab Co., Ltd. (Sengzhi-) * 6 Manufactured by Nikko Chemical Co., Ltd. (Production Method) A·Components (1) to (7) were mixed and heated and dissolved at 70 °C. B.  The components (8) to (1 2) are dissolved by mixing. C.  The components (13) to (15) are dissolved by mixing. D · Add B to A, mix and cool, then mix c to obtain a mask. Examples 3 - 6 have a moderately cool feeling and a feeling of tension, and the skin after the mask has a smooth feeling and a feeling of elasticity, and is a mask which can maintain the smoothness of the skin. -61 - 200808332 (58) (Example 3-7: Cream Foundation) (ingredient) (%) (1) sorbitan sesquioleate 3. 0 (2) Self-emulsified glyceryl monostearate 2. 0 (3) aluminum stearate 0. 5 (4) pure ground wax 5. 0 (5) 30-carbon institute 10. 0 (6) Mobile sarcophagus 15. 0 (7) Vaseline 5. 0 (8) titanium oxide 8. 0 (9) talc 4. 0 (1 〇 ) coloring pigment Appropriate amount (1 1 ) p-oxybenzoic acid methyl ester 〇. 〇5 (12) propylene glycol 5. 0 (1 3 ) Honeysuckle Extract * 1 1. 0 (14) White birch extract *2 1. 0 (15) Horse chestnut extract * 3 1. 0 (16) Birch sap*4 10. 0 (1 7 ) Remaining amount of pure water (1 8 ) Proper amount of spices *1 manufactured by Nagaoka Spice Co., Ltd. * 2 manufactured by Jiu-Sen Pharmaceutical Co., Ltd. *3 manufactured by Fraser Corporation *4 Saab Co., Ltd. (Sen Wei) -62- ( 59) (59)200808332 (Method of Production) A. The components (1) to (11) were heated and mixed, and kept at 70 °C. B·Components (12) to (17) were kept at 70 ° C after heating and mixing. C.  Emulsification was carried out after adding B to A. D. After cooling C, the component (18) was added and mixed to obtain a cream foundation. (Example 3 - 8 : Liquid foundation) (ingredient) (%) (1) Liquid aqueous lanolin 2. 0 (2) mobile paraffin 5. 0 (3) stearic acid 2. 0 (4) cetyl alcohol 1. 0 (5) Self-emulsified glyceryl monostearate 1. 0 (6) p-methoxycinnamic acid-2-ethylhexyl ester 8. 0 (7) 4-Terbutyl- 4'-methoxydibenzoylmethane methane 2. 0 (8) palmitic rosin oil * 1 0. 2 (9) p-oxybenzoic acid methyl ester 0. 1 (1 〇) glycerol 5. 0 (1 1 ) triethanolamine 1. 0 (12) carboxymethyl cellulose 0. 2 (13) Bentnite 0. 5 (1 4 ) White birch extract * 2 5. 0 (1 5 ) pure water residual amount (1 6 ) titanium oxide -63- 200808332 (60) (1 7 ) fine particle titanium oxide 2 · 0 (1 8 ) fine particle zinc oxide 5. 0 (1 9 ) mica 2. 0 4. 0 4. 0 0. 1 5. 0 Appropriate amount (2 0 ) Talc (2 1 ) Coloring pigment (22) Mannitol * 3 (23 ) Birch sap * 4 (24 ) Asparagus extract * (2 5 ) Licorice extract * (26 ) Spice * 1 曰罗罗修公司制氺2 Jiushan Pharmaceutical Co., Ltd.* 3 S i gm [a company 氺4 Shabu company system (Senzhi zero) * 5 Jiushan Pharmaceutical Co., Ltd. 6 Jiushan Pharmaceutical Co., Ltd. (Manufacturing) A . The components (1) to (9) were dissolved by heating. Add the ingredients (1 6 ) to (2 1 ) to B · A and mix them evenly to maintain 70 °C. C. The components (10) to (15) were dissolved by heating and kept at 70 °C. D.  Emulsification was carried out uniformly after adding B to C. E. After cooling D, components (22) to (26) were added and mixed to obtain a liquid foundation. -64-200808332 (61) Examples 3-7 and 3-8 have a strong and smooth feeling of use, and can provide a uniform and aesthetically pleasing makeup effect, and are a cream foundation and a liquid foundation which can maintain a smooth skin. (Examples 3-9: sunscreen lotion) (ingredient) (%) (1) polyoxyalkylene-denatured organic polyoxyalkylene 1 ·〇(2) dimethylpolyoxyalkylene 5·0 (3 ) Methylcyclotetraoxane 20·〇(4)isotridecyl isononanoate 5·〇(5)p-methoxycinnamic acid-2-ethylhexyl ester 5·〇(6)-p-oxybenzoin Methyl ester 〇·1 (7) Polyfluorene treatment of fine particles of titanium oxide 1 〇· 〇 (8 ) Polyoxane treatment of fine particles zinc oxide 1 〇 · 〇 (9) polystyrene powder 3. 0 (10) Trimethylmethoxine hydroxamic acid 0. 5 (1 1 ) dipropylene glycol 3. 0 (1 2 ) Ethanol 10. 0 (13) Birch sap *1 1〇. 〇 (14) salt 0. 2 (1 5 ) Pure water Remaining amount (16) Cold-Carotene* 2 〇·〇1 (1 7) Chamomile extract* 3 1. 0 -65 200808332 (62) (1 8 ) My Red Extract* 4 1. 0 (1 9 ) Proper amount of spices *1 Shabu company system (Mori no Yu) *2 Sigma company system *3 Jiushan Pharmaceutical Co., Ltd. *4 Jiushan Pharmaceutical Co., Ltd. (manufacturing method) A·Component (1)~(10) After heating and mixing, it was kept at 70 °C. B. The components (1 1 ) to (15) were kept at 70 ° C after heating and mixing. C.  Emulsification was carried out after adding B to A. D.  After cooling C, components (16) to (19) were added and mixed to obtain a sunscreen lotion. Examples 3-9 have a non-sticky and smooth feeling of use, and can protect the skin by ultraviolet rays and also maintain a smooth sunscreen lotion. (Example 4-1: lotion) (ingredient) (%) (1) glycerin 3. 0 (2) 1,3-butanediol 2. 0 (3) propylene glycol 4. 0 (4) oleyl alcohol 〇·1 (5) monolauric acid polyethylene oxide (20 Ε. Ο·) Sorbitol anhydride -66 - 200808332 (63) (6) Polyethylene oxide (20Ε·Ο·) lauryl ether 0·5 (7) triacontane 0. 5 (8) Ethanol 20. 0 (9) p-oxybenzoic acid methyl ester 0. 1 (10) acetic acid dl-α-tocopherol* 1 0. 5 (1 1 ) Spice Appropriate amount (12) Dipotassium glycyrrhizinate*2 0. 5 (1 3 ) Rosemary Extract * 3 0. 5 (14 ) Sodium dl-pyrrolidone carboxylic acid * 4 0. 2 (15) locust bean gum 1. 0 (1 6 ) Coix seed extract * 5 0. 5 (17) Birch sap* 6 20. 0 (1 8 ) Remaining amount of pure water * 1 made by Eisai Co., Ltd. *2 manufactured by Jiu-Sen Pharmaceutical Co., Ltd. *3 manufactured by Jiu-Sen Pharmaceutical Co., Ltd. * 4 Ajinomoto Co., Ltd. *5 Nine-Good Pharmaceutical Co., Ltd. *6 Saab Co., Ltd. -) (Processing method) A · The components (1) to (1 1 ) are dissolved by mixing. B · The components (1 2 ) to (1 8 ) are dissolved by mixing. C.  B is added to A and uniformly mixed to obtain a lotion. -67- 200808332 (64) Example 4-1 has a refreshing feeling of use in water, and it is possible to obtain a lotion that is free from rough skin or dull skin. (Example 4-2: Cream) (ingredient) (%) (1) Triacontan 20. 0 (2) beeswax (3) jojoba oil 5. 0 (4) glyceryl monostearate 2·0 (5) monostearic acid polyethylene oxide (20 Ε. Hey. ) sorbitol 2. 0 (6) sorbitan monostearate 2. 0 (7) plant alcohol * 1 0. 5 (8) Candid wax 1. 0 (9) glycerol 5. 0 (10) p-oxybenzoic acid methyl ester 〇·1 (1 1 ) lily extract * 2 1. 0 (1 2 ) ginseng extract * 3 1. 0 (1 3 ) Ginkgo Biloba Extract * 4 1. 0 (1 4 ) jujube extract * 5 1. 0 (1 5 ) Sophora flavescens extract * 6 1. 0 (1 6 ) Hydrolyzed Rice Extract* 7 1. 0 (1 7 ) Licorice Extract * 8 1. 0 (1 8 ) Angelica extract * 9 1. 0 (1 9 ) Birch sap * 1 0 5. 0 -68- 200808332 (65) (20) Remaining amount of pure water (21) Flavor amount 氺1 Eisai company 氺2 Jiushan Pharmaceutical Co., Ltd. 3 Jiushan Pharmaceutical Co., Ltd. 磐4 磐 磐 磐 磐 公司 公司5 九善制药制制制制6 Jiushan Pharmaceutical Co., Ltd.*7 TECHNO-BULL Company 氺8 Jiushan Pharmaceutical Co., Ltd. 氺9 Japan Powder Pharmaceutical Co., Ltd.*10 Saab Co., Ltd. (Mori Zero) (Manufacturing Method) Α· The components (1) to (10) were kept at 70 ° C after heating and mixing. B·Components (1 1 ) to (20) were kept at 70 ° C after heating and mixing. C.  Emulsification was carried out after adding B to A. D. After cooling C, the component (2 1 ) was added and mixed to obtain a cream. The 4-2 is a cream that has a smooth and strong feeling of use and can be used for beautiful skin without rough skin or dull skin. (Example 4-3: Cleansing Cream) (Ingredient) (%) -69- 200808332 (66) (1) Paraffin (2) Beeswax (3) Vaseline (4) Flowing paraffin (5) Loss of sorbitol Semi-oleate (6) monooleic acid polyethylene oxide (20E. O·) Sorbitol^ (7) Hydrogenated Soy Phospholipids (8) White Foams (Meadowfoam) *1 (9) Methyloxybenzoate (1 〇) 桴 seed extract* 2 (1 1) Licorice extract * 3 (1 2 ) Large sunflower flower extract * 4 (1 3 ) White birch sap * 5 (1 4 ) Pure water (1 5 ) Spice 氺 1 曰 界面 界面 界面 界面 2 2 2香荣兴业制制氺3 Jiushan Pharmaceutical Co., Ltd. 氺4 Jiushan Pharmaceutical Co., Ltd. 氺5 Saab Company (Sengzhi) (Method) A. The components (1) to (9) were heated and mixed to maintain 7 CTC. B · The components (1 0 ) to (1 4 ) were kept at 70 ° C after heating and mixing. 10. 0 3. 0 15. 0 41. 0 4. 2 0. 8 0. 5 1 . 0 0. 1 1 . 0 1. 0 1. 0 5. 0 Residual amount Appropriate amount -70- 200808332 (67) C.  Emulsification was carried out after adding B to A. D.  After cooling C, the ingredients (15) were added and mixed to obtain a cleansing cream. Example 4-3 has a light and smooth feeling of use, has good fusion with makeup, and has excellent cleansing and decontaminating properties, and can provide a cleansing cream for beautiful skin without rough skin or dull skin. (Example 4-4: Emulsion) (ingredient) (%) (1) Sorbitan monostearate 0. 3 (2) monooleic acid polyethylene oxide (20E. O. ) sorbitol 0. 1 (3) lipophilic glyceryl monostearate 0. 2 (4) stearic acid 0. 5 (5) cetyl alcohol 0. 5 (6) 30-carbon institute 3. 0 (7) mobile paraffin 4. 0 (8) vegetal triacontan* 1 0. 2 (9) Tris-2-ethylhexanoic acid glyceride 2. 0 (10) dimethyl polyoxane 1·0 (1 1 ) hydrogenated soybean phospholipid 〇 · 1 (12) niacin dl-α-tocopherol * 2 0. 05 (1 3 ) p-oxybenzoic acid ethyl ester 0 · 1 (14) carboxyvinyl polymer 0. 1 (15) sodium hydroxide 0. 05 -71 - 200808332 (68) (1 6 ) Glycerin 5. 0 (17 ) 1,3·Butanediol 7·0 (1 8 ) Pure water Remaining amount (19) Birch sap*3 10. 0 (20) Peony Extract *4 2. 0 (2 1 ) Ganoderma lucidum extract * 5 2. 0 (22) sodium hyaluronate 1% aqueous solution* 6 5. 0 (23) Ethyl glutamic acid * 7 0. 3 (24) Ethanol 5. 0 (2 5 ) porous ceria 3. 0 (26) Proper amount of spices*1 Manufactured by Nikko Chemical Co., Ltd. 2 Eisai Co., Ltd.*3 Saab Co., Ltd. (Mori no 雫) *4 Manufactured by Nine Good Pharmaceuticals Co., Ltd. 5 Manufactured by Nine Good Pharmaceutical Co., Ltd. 6 manufactured by Nikko Chemical Co., Ltd. * 7 Concord Fermentation Company (Manufacturing Method) A.  The components (14) to (23) were heated and mixed, and kept at 70 °C. B.  The components (1) to (13) were kept at 70 ° C after heating and mixing. C.  Emulsification was carried out after adding B to A. D.  After cooling C, ingredients (24), (25), (26) were added and -72-200808332 (69) was added to obtain an emulsion. (Example 4-5: Emulsion) (ingredient) (%) (1) Sorbic acid sorbitan 0. 3 (2) monooleic acid polyethylene oxide (20E. O·) sorbitol 0. 1 (3) lipophilic glyceryl monostearate 0. 2 (4) stearic acid 0. 5 (5) Sixteen hospitals alcohol 0. 5 (6) triacontane 3. 0 (7) mobile paraffin 4. 0 (8) tris-2-ethylhexanoic acid glyceride 2. 0 (9) dimethyl polyoxane 1. 0 (1 〇) hydrogenated soybean phospholipid 〇·1 (11) rutin* 1 〇. 2 (1 2 )-p-oxybenzoic acid methyl ester 0. 1 (13) Carboxyvinyl polymer 0. 1 (14) sodium hydroxide 0. 05 (1 5 ) glycerol 5. 0 (16) 1,3-butanediol 7. 0 (17) Birch sap*2 3. 0 (1 8 ) pure water remaining amount (1 9 ) yeast extract * 3 0. 5 (20) Tomorrow's Leaf Extract* 4 0. 5 -73- 200808332 (70) 0. 5 5. 0 3. 0 Appropriate amount (2 1 ) Hop extract* 5 (22) Peach extract* 6 (23) Ethanol (24) Porous cerium oxide (25) Spice 氺1 Sigma company*2 Saab company system (Sengzhi —) 氺3 BIO-DELL Company 氺4 九善制药股份有限公司*5 九善制药公司制氺6 Jiushan Pharmaceutical Co., Ltd. (manufacturing method) A·Component (13)~(22) After heating and mixing, keep 70°C . B·Components (1) to (12) were kept at 70 ° C after heating and mixing. After adding B to C · A, the emulsion was uniformly emulsified. D. After cooling C, components (23), (24), and (25) are added and mixed to obtain an emulsion. In Example 4-4 and Example 4-5, a smooth and soft feeling of use was obtained, and an emulsion of beautiful skin without rough skin or dull skin was obtained. (%) (Example 4-6: Mask) (Component) -74- 200808332 (71) (1) Polyvinyl alcohol 13. 0 (2) Anhydrous citric acid 0. 5 (3) Polyethylene glycol 0. 5 (4) White Mango Seed Oil 1 (Meadowfoam) *1 0. 2 (5) Polypropylene oxide (10P. Hey. )methylglucoside 5. 0 (6) glycerol 5. 0 (7) pure water remaining amount (8) tea extract * 2 1. 0 (9) Essential Oil Rose Extract* 3 1. 0 (1 0) Anhydrous caffeine* 4 1. 0 (1 1 ) Birch sap* 5 5. 0 (12) Phosphate-L-ascorbate*6 3. 0 (1 3 ) pure water 10. 0 (14) p-oxybenzoic acid ethyl ester 0. 1 (1 5 ) Ethanol 1 3 · 0 (1 6 ) Proper amount of 氺 1 曰 界面 界面 界面 界面 九 九 九 九 九 九 九 九 九 九 九 九 九 九 九 九 九 九 九 九 九 纯 纯 纯 纯 纯 纯 纯 纯 纯 纯 纯布 公司 ( 森 曰 曰 曰 曰 曰 曰 曰 曰 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008 B. The components (8) to (1 3) are dissolved by mixing. C. The components (14) to (16) are dissolved by mixing. D · Add B to A, mix and cool, then add c and mix to obtain mask D. Add B to A, mix and cool, then add c and mix to get mask. Example 4-6 has moderate coolness and tension. The skin after the mask has elasticity. It is a mask for beautiful skin without rough skin or dull skin. (Example 4-7: Cream Foundation) (ingredient) (〇/〇) (1) sorbitan sesquioleate 3〇 (2) Self-emulsified glyceryl monostearate 2. 0 (3) aluminum stearate 0. 5 (4) pure ground wax 5. 0 (5) triacontane 10. 0 (6) Mobile sarcophagus 15. 0 (7) Vaseline 5. 0 (8) titanium oxide 8 .  〇 (9) talc 4. 0 (1 〇 ) coloring pigment Appropriate amount (11 ) p-oxybenzoic acid methyl ester 0. 05 (12) Propylene glycol 5. 0 -76- 200808332 (73) (1 3 ) Honeysuckle Extract * 1 1. 0 (14) White birch extract *2 1. 0 (1 5 ) Horse chestnut extract * 3 1. 0 (16) Birch sap*4 10. 0 (1 7 ) Remaining amount of pure water (1 8 ) Proper amount of spices * 1 manufactured by Nagaoka Spice Co., Ltd. *2 manufactured by Kyusan Pharmaceutical Co., Ltd. *3 manufactured by Fraser Corporation * 4 Saab Co., Ltd. (Mori no Kiseki) (Method) A - The components (1) to (1 1) are heated and mixed to maintain 7 (TC. B. The components (12) to (17) are heated and mixed to maintain 7 (TC. C.  Emulsification was carried out after adding B to A. D. After cooling C, the component (18) was added and mixed to obtain a cream foundation. (Example 4-8: Liquid foundation) (ingredient) (%) (1) Liquid aqueous lanolin 2. 0 (2) mobile paraffin 5. 0 (3) stearic acid 2. 0 (4) Cetyl alcohol 1·〇 (5) Self-emulsified glyceryl monostearate 1. 0 -77 200808332 (74) (6) p-methoxycinnamic acid-2-ethylhexyl ester 8. 0 (7) 4-Tert-butyl-4'-methoxybenzhydrylmethane 2. 0 (8) palmitic rosin oil * 1 0. 2 (9) p-oxybenzoic acid methyl ester 0. 1 (1 〇) glycerol 5. 0 (1 1 ) triethanolamine 1. 0 (12) carboxymethyl cellulose 0. 2 (13) Bentnite 0. 5 (14) White birch extract *2 5. 0 (1 5 ) pure water remaining amount (16) mannitol *3 0. 1 (17) Birch sap*4 5. 0 (1 8 ) Asparagus extract * 5 0. 5 (19) Licorice Extract *6 0. 5 (20) titanium oxide 6. 0 (21) microparticle titanium oxide 2. 0 (22) micronized zinc oxide 5. 0 (23) mica 2. 0 (24) Talc 4. 0 (2 5 ) coloring pigment 4. 0 (26) Proper amount of spices*1 Made by Japan Rosho Corporation*2 Manufactured by Jiushan Pharmaceutical Co., Ltd.*3 Sigma-78-200808332 (75) *4 Shabu Company (Senzhi-) *5 Manufactured by Jiu-Zhong Pharmaceutical Co., Ltd. *6 Jiushan Pharmaceutical Co., Ltd. (Manufacturing Method) A.  The components (1) to (9) are dissolved by mixing. B.  Add components (20) to (25) to A and mix them evenly and keep at 70 °C. C.  The ingredients (1〇)~(19) were uniformly dissolved and kept at 70 °C. ^ D .  Emulsification was carried out after adding B to C. E. After cooling D, component (26) was added and mixed to obtain a liquid foundation. Each of Examples 4-7 and 4-8 has a strong and smooth feeling of use, and a uniform and beautiful makeup effect can be obtained, and it is a cream foundation and a liquid foundation which can obtain beautiful skin without rough skin or dull skin. (Example 4-9: sunscreen lotion) (ingredient) (%) (1) polyoxyalkylene-denatured organic polyoxyalkylene 1. 〇 (2) dimethyl polyoxane 5. 0 (3) octamethylcyclotetraoxane 20. 0 (4) isotridecyl isononate 5. 0 (5 ) p-methoxycinnamic acid-2-ethylhexyl ester 5. 0 (6 ) p-oxybenzoic acid methyl ester 0. 1 (7) Polyfluorene-treated microparticulate titanium oxide 10. 0 -79- 200808332 (76) (8) Polyfluorene treatment of micronized zinc oxide 10. 0 (9) Polystyrene benzene powder 3. 0 (10) Trimethylmethoxine hydroxamic acid 0. 5 (1 1 ) dipropylene glycol 3. 0 (1 2 ) Ethanol 10. 0 (13) Birch sap*1 10. 0 (14) salt 0·2 (1 5 ) pure water remaining amount (16 ) /3 - carotene * 2 0. 01 (1 7 ) Chamomile Extract * 3 1. 0 (1 8 ) My Red Extract* 4 1. 0 (1 9 ) Spices Appropriate amount *1 Shabu company system (Sengzhi-) *2 Sigma company system *3 Jiushan Pharmaceutical Co., Ltd. *4 Jiushan Pharmaceutical Co., Ltd. (Manufacturing Method) A. Mix the ingredients (1) to (1 0) and keep 7 (ΓC. B·mixed components (11) to (15) and keep 7 (TC.C.  After adding B to A, it was uniformly emulsified. D. After cooling C, ingredients (1 6 ) to (1 9 ) are added and mixed to obtain a sunscreen lotion. -80- 200808332 (77) Example 4-9 has a non-sticky and smooth feeling of use, and can protect the skin with ultraviolet rays, and can obtain a sunscreen lotion of beautiful skin without rough skin or dull skin. [Simplified illustration] [Fig. 1] Test results of epidermal cell keratinization promoting effect; cell morphology when adding white birch sap 1 〇% (Example 1) [Fig. 2] Test results of epidermal keratinization promoting effect; Cell morphology at the time (Comparative Example 1) [Fig. 3] Test results of epidermal cell keratinization promoting effect; addition 1 Cell morphology at 5 mM calcium chloride (Comparative Example 2) [Fig. 4] Test for promoting keratinization of epidermal cells; comparison of viable cells by MTTas say [Fig. 5] Results of cell proliferation promotion test -81 -

Claims (1)

200808332 (1) 十、申請專利範圍 1 · 一種原聚角微絲蛋白（p r 〇 f i 1 a g g r i η )或聚角微絲蛋 白（filaggrin )產生促進劑，其特徵爲以白樺（Betula platyphylla Sukatche v var. j aponica Hara )樹液作爲有效 成分者。 2· *—種使用’其特徵爲將白棒（Betula platyphylla Sukatchev var. japonica Hara)樹液作爲原聚角微絲蛋白 (pro filaggrin )或聚角微絲蛋白（filaggrin )產生促進劑 之使用。 3·—種包殼蛋白（involucriii)產生促進劑，其特徵爲 將白樺(Betula platyphylla Sukatchev var. japonica Hara )樹液作爲有效成分者。 4. 一種使用，其特徵爲將白樺（Betula platyphylla Sukatchev var. japonica Hara)樹液作爲包殼蛋白（ involucrin)產生促進劑之使用。 5. —種膽固醇產生促進劑，其特徵爲將白樺（Betula platyphylla Sukatchev var. japonica Hara)樹液作爲有效 成分者。 6. —種使用，其特徵爲將白樺（Betula platyphylla Sukatchev var. japonica Hara)樹液使用於膽固醇產生促 進劑之使用。 7. —種細胞賦活劑，其特徵爲將白樺（BetuU platyphylla Sukatchev var. japonica Hara)樹液作爲有效 成分者。 -82- 200808332 (2) 8. — 種{ S ukatchev v 用。 用，其特徵爲將白樺（Betulaplatyphylla r.japonicaHara)樹液作爲細胞賦活劑之使 -83-200808332 (1) X. Patent application scope 1 · A pro-aggregated microfilament protein (pr 〇fi 1 aggri η ) or a filaggrin production promoter, characterized by white birch (Betula platyphylla Sukatche v var j aponica Hara ) sap as an active ingredient. 2. The use of a 'betula platyphylla Sukatchev var. japonica Hara' sap is used as a promoter for pro filaggrin or filaggrin production. 3. Involucriii production promoter, characterized by Betula platyphylla Sukatchev var. japonica Hara sap as an active ingredient. 4. A use characterized by the use of Betula platyphylla Sukatchev var. japonica Hara sap as an involucrin production promoter. A cholesterol production promoter characterized by using a sap of Betula platyphylla Sukatchev var. japonica Hara as an active ingredient. 6. Use as characterized by the use of Betula platyphylla Sukatchev var. japonica Hara sap for the use of a cholesterol generating promoter. 7. A cell activator characterized by using a sap of BetuU platyphylla Sukatchev var. japonica Hara as an active ingredient. -82- 200808332 (2) 8. — Kind of { S ukatchev v. Use, which is characterized by the use of Betulaplatyphylla r. japonicaa sap as a cell activator -83-