RU95105899A - Способ иммобилизации фермента, полинуклеотид, вектор, химерный блок, низшие эукариоты, способ осуществления ферментативного процесса - Google Patents

Способ иммобилизации фермента, полинуклеотид, вектор, химерный блок, низшие эукариоты, способ осуществления ферментативного процесса

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Publication number
RU95105899A
RU95105899A RU95105899/13A RU95105899A RU95105899A RU 95105899 A RU95105899 A RU 95105899A RU 95105899/13 A RU95105899/13 A RU 95105899/13A RU 95105899 A RU95105899 A RU 95105899A RU 95105899 A RU95105899 A RU 95105899A
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RU
Russia
Prior art keywords
enzyme
polynucleotide
vector
gene encoding
chimeric protein
Prior art date
Application number
RU95105899/13A
Other languages
English (en)
Inventor
Мария Клис Франсискус
Nl]
Плен Шройдер Мартен
Йорк Тошка Холгер
Теодорус Веррипс Корнелис
De]
Original Assignee
Унилевер Н.В. (NL)
Унилевер Н.В.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Унилевер Н.В. (NL), Унилевер Н.В. filed Critical Унилевер Н.В. (NL)
Publication of RU95105899A publication Critical patent/RU95105899A/ru

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2465—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on alpha-galactose-glycoside bonds, e.g. alpha-galactosidase (3.2.1.22)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/16—Enzymes or microbial cells immobilised on or in a biological cell
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/035—Fusion polypeptide containing a localisation/targetting motif containing a signal for targeting to the external surface of a cell, e.g. to the outer membrane of Gram negative bacteria, GPI- anchored eukaryote proteins
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals

Abstract

Сущность изобретения: способ иммобилизации фермента предусматривает иммобилизацию фермента или его функциональной части на стенке микробной клетки с использованием техники рекомбинантных ДНК. Иммобилизацию фермента осуществляют путем его сшивания с С-концевой частью белка, обеспечивающего прикрепление к клеточной стенке. Изобретение также относится к рекомбинантному полинуклеотиду, содержащему структурный ген, кодирующий ферментный белок, часть гена, кодирующего С-концевую часть белка, способного закрепляться в эукариотической или прокариотической стенке, а также сигнальную последовательность в дополнение к химерному белку, кодированному рекомбинантным полинуклеотидом. Кроме того, изобретение относится к вектору и микроорганизму, содержащим указанный полинуклеотид. Указанный микроорганизм может быть использован для осуществления ферментативных процессов в промышленном масштабе.

Claims (1)

  1. Сущность изобретения: способ иммобилизации фермента предусматривает иммобилизацию фермента или его функциональной части на стенке микробной клетки с использованием техники рекомбинантных ДНК. Иммобилизацию фермента осуществляют путем его сшивания с С-концевой частью белка, обеспечивающего прикрепление к клеточной стенке. Изобретение также относится к рекомбинантному полинуклеотиду, содержащему структурный ген, кодирующий ферментный белок, часть гена, кодирующего С-концевую часть белка, способного закрепляться в эукариотической или прокариотической стенке, а также сигнальную последовательность в дополнение к химерному белку, кодированному рекомбинантным полинуклеотидом. Кроме того, изобретение относится к вектору и микроорганизму, содержащим указанный полинуклеотид. Указанный микроорганизм может быть использован для осуществления ферментативных процессов в промышленном масштабе.
RU95105899/13A 1992-07-08 1993-07-07 Способ иммобилизации фермента, полинуклеотид, вектор, химерный блок, низшие эукариоты, способ осуществления ферментативного процесса RU95105899A (ru)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
NL92202080.5 1992-07-08
EP92202080 1992-07-08
EP92203899 1992-12-14
NL92203899.7 1992-12-14

Publications (1)

Publication Number Publication Date
RU95105899A true RU95105899A (ru) 1997-03-20

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
RU95105899/13A RU95105899A (ru) 1992-07-08 1993-07-07 Способ иммобилизации фермента, полинуклеотид, вектор, химерный блок, низшие эукариоты, способ осуществления ферментативного процесса

Country Status (16)

Country Link
US (1) US6027910A (ru)
EP (1) EP0673427B1 (ru)
JP (2) JP3681385B2 (ru)
KR (1) KR950702634A (ru)
AT (1) ATE236257T1 (ru)
AU (1) AU685057B2 (ru)
CA (1) CA2139670C (ru)
DE (1) DE69332829T2 (ru)
DK (1) DK0673427T3 (ru)
ES (1) ES2196009T3 (ru)
FI (1) FI950042A (ru)
NZ (1) NZ254108A (ru)
OA (1) OA10123A (ru)
PT (1) PT673427E (ru)
RU (1) RU95105899A (ru)
WO (1) WO1994001567A1 (ru)

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Also Published As

Publication number Publication date
PT673427E (pt) 2003-08-29
WO1994001567A1 (en) 1994-01-20
OA10123A (en) 1996-12-18
EP0673427A1 (en) 1995-09-27
AU4565393A (en) 1994-01-31
DK0673427T3 (da) 2003-07-21
KR950702634A (ko) 1995-07-29
JP2004254700A (ja) 2004-09-16
ES2196009T3 (es) 2003-12-16
FI950042A (fi) 1995-01-24
US6027910A (en) 2000-02-22
EP0673427B1 (en) 2003-04-02
AU685057B2 (en) 1998-01-15
JPH07508652A (ja) 1995-09-28
FI950042A0 (fi) 1995-01-04
NZ254108A (en) 1995-12-21
JP3681385B2 (ja) 2005-08-10
CA2139670C (en) 2005-09-27
DE69332829T2 (de) 2004-01-22
DE69332829D1 (de) 2003-05-08
CA2139670A1 (en) 1994-01-20
ATE236257T1 (de) 2003-04-15

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