PT824598E - Processo para o acoplamento especifico de um grupo de bloqueio da extremidade 5' de um fragmento de arnm e preparacao do arnm e do adnc completo - Google Patents

Processo para o acoplamento especifico de um grupo de bloqueio da extremidade 5' de um fragmento de arnm e preparacao do arnm e do adnc completo

Info

Publication number
PT824598E
PT824598E PT96914259T PT96914259T PT824598E PT 824598 E PT824598 E PT 824598E PT 96914259 T PT96914259 T PT 96914259T PT 96914259 T PT96914259 T PT 96914259T PT 824598 E PT824598 E PT 824598E
Authority
PT
Portugal
Prior art keywords
mrna
pct
preparation
fragment
specific coupling
Prior art date
Application number
PT96914259T
Other languages
English (en)
Inventor
Irena Merenkova
Jean-Baptiste Gabriel Edwards
Original Assignee
Genset Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from FR9505221A external-priority patent/FR2733765B1/fr
Application filed by Genset Sa filed Critical Genset Sa
Publication of PT824598E publication Critical patent/PT824598E/pt

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Mechanical Coupling Of Light Guides (AREA)
  • Cosmetics (AREA)
  • Saccharide Compounds (AREA)
PT96914259T 1995-05-02 1996-04-29 Processo para o acoplamento especifico de um grupo de bloqueio da extremidade 5' de um fragmento de arnm e preparacao do arnm e do adnc completo PT824598E (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR9505221A FR2733765B1 (fr) 1995-05-02 1995-05-02 Methode de couplage specifique de la coiffe de l'extremite 5' d'un fragment d'arnm
FR9509467A FR2733762B1 (fr) 1995-05-02 1995-08-03 Methode de couplage specifique de la coiffe de l'extremite 5' d'un fragment d'arnm et preparation d'arnm et d'adnc complet

Publications (1)

Publication Number Publication Date
PT824598E true PT824598E (pt) 2003-03-31

Family

ID=26231928

Family Applications (1)

Application Number Title Priority Date Filing Date
PT96914259T PT824598E (pt) 1995-05-02 1996-04-29 Processo para o acoplamento especifico de um grupo de bloqueio da extremidade 5' de um fragmento de arnm e preparacao do arnm e do adnc completo

Country Status (12)

Country Link
US (1) US6022715A (pt)
EP (1) EP0824598B1 (pt)
JP (1) JPH11510364A (pt)
AT (1) ATE227346T1 (pt)
AU (1) AU719217B2 (pt)
CA (1) CA2220045C (pt)
DE (1) DE69624695T2 (pt)
DK (1) DK0824598T3 (pt)
ES (1) ES2186778T3 (pt)
FR (1) FR2733762B1 (pt)
PT (1) PT824598E (pt)
WO (1) WO1996034981A2 (pt)

Families Citing this family (90)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5766891A (en) 1994-12-19 1998-06-16 Sloan-Kettering Institute For Cancer Research Method for molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase
JP3441899B2 (ja) * 1996-11-01 2003-09-02 理化学研究所 完全長cDNAライブラリーの作成方法
WO1998056943A1 (en) 1997-06-12 1998-12-17 Sloan-Kettering Institute For Cancer Research Covalent joining of dna strands to rna strands catalyzed by vaccinia topoisomerase
CA2297157A1 (en) * 1997-08-01 1999-02-11 Genset 5' ests for secreted proteins expressed in testis and other tissues
WO1999006548A2 (en) * 1997-08-01 1999-02-11 Genset 5'ESTs FOR NON TISSUE SPECIFIC SECRETED PROTEINS
US7393663B2 (en) 1997-08-01 2008-07-01 Serono Genetics Institute S.A. Expressed sequence tags and encoded human proteins
EP1000152A2 (en) * 1997-08-01 2000-05-17 Genset 5' ESTs FOR SECRETED PROTEINS EXPRESSED IN MUSCLE AND OTHER MESODERMAL TISSUES
CA2296287A1 (en) * 1997-08-01 1999-02-11 Genset 5' ests for secreted proteins expressed in prostate
US6222029B1 (en) 1997-08-01 2001-04-24 Genset 5′ ESTs for secreted proteins expressed in brain
DK1000149T3 (da) * 1997-08-01 2007-09-10 Serono Genetics Inst Sa 5'-EST'ere til udskilte proteiner identificeret fra hjernevæv
CA2296398A1 (en) * 1997-08-01 1999-02-11 Genset 5' ests for secreted proteins expressed in endoderm
PT1375514E (pt) * 1997-08-01 2006-10-31 Serono Genetics Inst Sa Ests 5' para proteinas secretadas expressas em varios tecidos
FR2767135B1 (fr) 1997-08-06 2002-07-12 Genset Sa Recepteur complexe lsr, activite, clonage, et application au diagnostic, a la prevention et/ou au traitement de d'obesite et des risques ou complications associes
EP1026242A1 (en) * 1997-10-22 2000-08-09 Helix Research Institute METHOD FOR SCREENING FULL-LENGTH cDNA CLONES
US6548633B1 (en) 1998-12-22 2003-04-15 Genset, S.A. Complementary DNA's encoding proteins with signal peptides
WO1999040189A2 (en) * 1998-02-09 1999-08-12 Genset Cdnas encoding secreted proteins
US20060286639A1 (en) * 1997-11-13 2006-12-21 Serono Genetics Institute S.A. Complementary DNAs encoding proteins with signal peptides
JP2001523453A (ja) * 1997-11-13 2001-11-27 ジェンセット 分泌タンパク質の伸長cDNA
EP1903111A3 (en) * 1997-11-13 2008-07-02 Serono Genetics Institute S.A. Extended cDNAs for secreted proteins
US6573068B1 (en) 1997-11-13 2003-06-03 Genset, S. A. Claudin-50 protein
CA2311572A1 (en) * 1997-12-17 1999-06-24 Genset S.A. Extended cdnas for secreted proteins
US7223727B2 (en) 1998-04-09 2007-05-29 Serono Genetics Institute S.A. GSSP4 polynucleotides and polypeptides and uses thereof
US6783961B1 (en) 1999-02-26 2004-08-31 Genset S.A. Expressed sequence tags and encoded human proteins
EP1068312A2 (en) 1998-04-09 2001-01-17 Genset 5' ests and encoded human proteins
WO2000026363A1 (en) 1998-11-04 2000-05-11 Genset GENOMIC AND COMPLETE cDNA SEQUENCES OF HUMAN ADIPOCYTE-SPECIFIC APM1 AND BIALLELIC MARKERS THEREOF
US6455280B1 (en) 1998-12-22 2002-09-24 Genset S.A. Methods and compositions for inhibiting neoplastic cell growth
AU767378B2 (en) 1999-02-10 2003-11-06 Genset Polymorphic markers of the LSR gene
EP1586645A3 (en) 1999-02-25 2006-02-22 Ceres Incorporated Sequence-determined DNA fragments and corresponding polypeptides encoded thereby
AU3397800A (en) * 1999-03-19 2000-10-09 Invitrogen Corporation Methods of obtaining full-length nucleic acid sequences using (e. coli) topoisomerase iii and its homologs
JP2002539799A (ja) * 1999-03-19 2002-11-26 ジェネティックス・インスチチュート・インコーポレーテッド プライマー結合ベクター伸長(PAVE):5’−指向cDNAクローニング法
US7485715B2 (en) 1999-06-18 2009-02-03 Ceres, Inc. Sequence-determined DNA encoding AP2 domain polypeptides
US7479555B2 (en) 1999-07-21 2009-01-20 Ceres, Inc. Polynucleotides having a nucleotide sequence that encodes a polypeptide having MOV34 family activity
CA2311201A1 (en) 1999-08-05 2001-02-05 Genset S.A. Ests and encoded human proteins
US6989367B2 (en) 2000-01-14 2006-01-24 Genset S.A. OBG3 globular head and uses thereof
US20020058617A1 (en) 2000-01-14 2002-05-16 Joachim Fruebis OBG3 globular head and uses thereof for decreasing body mass
US7338787B2 (en) 2000-01-14 2008-03-04 Serono Genetics Institute S.A. Nucleic acids encoding OBG3 globular head and uses thereof
US6566332B2 (en) 2000-01-14 2003-05-20 Genset S.A. OBG3 globular head and uses thereof for decreasing body mass
AU2001243232A1 (en) 2000-02-25 2001-09-03 University Of South Carolina Research Foundation Topoisomerase linker-mediated amplification methods
US6387624B1 (en) * 2000-04-14 2002-05-14 Incyte Pharmaceuticals, Inc. Construction of uni-directionally cloned cDNA libraries from messenger RNA for improved 3′ end DNA sequencing
US7033801B2 (en) 2000-12-08 2006-04-25 Invitrogen Corporation Compositions and methods for rapidly generating recombinant nucleic acid molecules
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
CA2421057A1 (en) 2000-08-21 2002-02-28 Invitrogen Corporation Methods and reagents for molecular cloning
US6875907B2 (en) 2000-09-13 2005-04-05 Pioneer Hi-Bred International, Inc. Antimicrobial peptides and methods of use
US20060008817A1 (en) 2000-12-08 2006-01-12 Invitrogen Corporation Methods and compositions for generating recombinant nucleic acid molecules
WO2003027243A2 (en) 2001-09-27 2003-04-03 Pioneer Hi-Bred International, Inc. Phytate polynucleotides and methods of use
EP1572927A4 (en) 2002-04-08 2007-10-17 Pioneer Hi Bred Int REINFORCED EXTRACTION OF SILK UNDER STRESS
WO2003106672A2 (en) * 2002-06-12 2003-12-24 Riken METHOD FOR UTILIZING THE 5'END OF mRNA FOR CLONING AND ANALYSIS
US8067205B2 (en) * 2003-03-28 2011-11-29 Japan As Represented By Director General Of National Rehabilitation Center For Persons With Disabilities Method of synthesizing cDNA
ATE469984T1 (de) 2003-12-01 2010-06-15 Life Technologies Corp Rekombinationsstellen enthaltende nukleinsäuremoleküle und verfahren zur verwendung davon
GB0417887D0 (en) * 2004-08-11 2004-09-15 Ares Trading Sa Protein
EP2062973B1 (en) * 2006-09-11 2014-11-19 Osaka University TRACE mRNA AMPLIFICATION METHOD AND USE THEREOF
CL2008003469A1 (es) 2007-11-20 2009-09-04 Pioneer Hi Bred Int Acido nucleico aislado de maiz asociado con la senalizacion de etileno en plantas; casete de expresion; celula huesped; proteina codificada; y metodo de modulacion de la respuesta de etileno en una planta.
US20100035249A1 (en) * 2008-08-05 2010-02-11 Kabushiki Kaisha Dnaform Rna sequencing and analysis using solid support
US8716553B2 (en) 2009-03-02 2014-05-06 Pioneer Hi Bred International Inc NAC transcriptional activators involved in abiotic stress tolerance
WO2011017492A2 (en) 2009-08-05 2011-02-10 Pioneer Hi-Bred International, Inc. Novel eto1 genes and use of same for reduced ethylene and improved stress tolerance in plants
CA2807552A1 (en) 2010-08-06 2012-02-09 Moderna Therapeutics, Inc. Engineered nucleic acids and methods of use thereof
CN103429606A (zh) 2010-10-01 2013-12-04 现代治疗公司 设计核酸及其使用方法
JP2014511687A (ja) 2011-03-31 2014-05-19 モデルナ セラピューティクス インコーポレイテッド 工学操作された核酸の送達および製剤
US9464124B2 (en) 2011-09-12 2016-10-11 Moderna Therapeutics, Inc. Engineered nucleic acids and methods of use thereof
RS62993B1 (sr) 2011-10-03 2022-03-31 Modernatx Inc Modifikovani nukleozidi, nukleotidi, i nukleinske kiseline, i njihove upotrebe
WO2013063006A1 (en) 2011-10-25 2013-05-02 Pioneer Hi-Bred International, Inc. Methods to alter plant cell wall composition for improved biofuel production and silage digestibility
SG10201604896TA (en) 2011-12-16 2016-08-30 Moderna Therapeutics Inc Modified nucleoside, nucleotide, and nucleic acid compositions
JP2015513913A (ja) 2012-04-02 2015-05-18 モデルナ セラピューティクス インコーポレイテッドModerna Therapeutics,Inc. 修飾ポリヌクレオチド
US9572897B2 (en) 2012-04-02 2017-02-21 Modernatx, Inc. Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins
US9283287B2 (en) 2012-04-02 2016-03-15 Moderna Therapeutics, Inc. Modified polynucleotides for the production of nuclear proteins
US9254311B2 (en) 2012-04-02 2016-02-09 Moderna Therapeutics, Inc. Modified polynucleotides for the production of proteins
EP2684954A1 (en) 2012-07-10 2014-01-15 Lexogen GmbH 5´ protection dependent amplification
US9422602B2 (en) * 2012-08-15 2016-08-23 Bio-Rad Laboratories, Inc. Methods and compositions for determining nucleic acid degradation
HRP20220607T1 (hr) 2012-11-26 2022-06-24 Modernatx, Inc. Terminalno modificirana rna
EP2983804A4 (en) 2013-03-15 2017-03-01 Moderna Therapeutics, Inc. Ion exchange purification of mrna
US8980864B2 (en) 2013-03-15 2015-03-17 Moderna Therapeutics, Inc. Compositions and methods of altering cholesterol levels
EP2971161B1 (en) 2013-03-15 2018-12-26 ModernaTX, Inc. Ribonucleic acid purification
BR112015023703A2 (pt) 2013-03-15 2017-07-18 Pioneer Hi Bred Int modulação de expressão de deaminase de acc
EP2971165A4 (en) 2013-03-15 2016-11-23 Moderna Therapeutics Inc DISSOLUTION OF DNA FRAGMENTS IN MRNA MANUFACTURING METHODS
WO2014152027A1 (en) 2013-03-15 2014-09-25 Moderna Therapeutics, Inc. Manufacturing methods for production of rna transcripts
EP3019619B1 (en) 2013-07-11 2021-08-25 ModernaTX, Inc. Compositions comprising synthetic polynucleotides encoding crispr related proteins and synthetic sgrnas and methods of use
US10023626B2 (en) 2013-09-30 2018-07-17 Modernatx, Inc. Polynucleotides encoding immune modulating polypeptides
US10385088B2 (en) 2013-10-02 2019-08-20 Modernatx, Inc. Polynucleotide molecules and uses thereof
CA2926218A1 (en) 2013-10-03 2015-04-09 Moderna Therapeutics, Inc. Polynucleotides encoding low density lipoprotein receptor
US11479766B2 (en) * 2013-12-05 2022-10-25 New England Biolabs, Inc. Methods for labeling a population of RNA molecules
AU2015289656A1 (en) 2014-07-16 2017-02-16 Modernatx, Inc. Circular polynucleotides
WO2017049286A1 (en) 2015-09-17 2017-03-23 Moderna Therapeutics, Inc. Polynucleotides containing a morpholino linker
WO2017066797A1 (en) 2015-10-16 2017-04-20 Modernatx, Inc. Trinucleotide mrna cap analogs
US20190218546A1 (en) 2015-10-16 2019-07-18 Modernatx, Inc. Mrna cap analogs with modified phosphate linkage
JP6417603B2 (ja) 2016-01-27 2018-11-07 株式会社ダナフォーム Rnaの末端領域に対応する核酸の塩基配列を解読する方法およびdnaエレメントの分析方法
WO2018053209A1 (en) 2016-09-14 2018-03-22 Modernatx, Inc. High purity rna compositions and methods for preparation thereof
WO2019036685A1 (en) 2017-08-18 2019-02-21 Modernatx, Inc. METHODS FOR HPLC ANALYSIS
WO2019036683A1 (en) 2017-08-18 2019-02-21 Modernatx, Inc. ANALYTICAL METHODS BY HPLC
WO2023127774A1 (ja) * 2021-12-28 2023-07-06 株式会社ダナフォーム 遺伝子包含体の検出方法及び検出用キット
WO2023237703A1 (en) 2022-06-09 2023-12-14 Vestlandets Innovasjonsselskap As Method of characterizing capped rna

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4661450A (en) * 1983-05-03 1987-04-28 Molecular Genetics Research And Development Limited Partnership Molecular cloning of RNA using RNA ligase and synthetic oligonucleotides
US4710566A (en) * 1984-12-27 1987-12-01 Yale University Purification of the messenger RNA cap-binding protein
US5273879A (en) * 1987-07-23 1993-12-28 Syntex (U.S.A.) Inc. Amplification method for polynucleotide assays
US5219989A (en) * 1988-12-13 1993-06-15 Mcgill University Bifunctional protein for the isolation of capped mRNA
CA2005252C (en) * 1988-12-13 2001-07-17 Nahum Sonenberg Isolation of full-length cdna clones and capped mrna
US5324829A (en) * 1988-12-16 1994-06-28 Ortho Diagnostic Systems, Inc. High specific activity nucleic acid probes having target recognition and signal generating moieties
JP3315439B2 (ja) * 1992-07-21 2002-08-19 住友電気工業株式会社 細胞特異的cDNAの分離法及びライブラリー作製法
JP3337748B2 (ja) * 1992-09-25 2002-10-21 財団法人神奈川科学技術アカデミー 完全長cDNAの合成方法、その中間体の製造方法及び完全長cDNAを含む組換えベクターの製造方法

Also Published As

Publication number Publication date
US6022715A (en) 2000-02-08
WO1996034981A3 (fr) 1997-01-03
DE69624695T2 (de) 2003-07-31
FR2733762A1 (fr) 1996-11-08
CA2220045C (fr) 2001-10-30
JPH11510364A (ja) 1999-09-14
AU719217B2 (en) 2000-05-04
AU5982996A (en) 1996-11-21
ES2186778T3 (es) 2003-05-16
EP0824598A2 (fr) 1998-02-25
ATE227346T1 (de) 2002-11-15
FR2733762B1 (fr) 1997-08-01
WO1996034981A2 (fr) 1996-11-07
EP0824598B1 (fr) 2002-11-06
CA2220045A1 (fr) 1996-11-07
DE69624695D1 (de) 2002-12-12
DK0824598T3 (da) 2003-03-10

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