AU2001243232A1 - Topoisomerase linker-mediated amplification methods - Google Patents

Topoisomerase linker-mediated amplification methods

Info

Publication number
AU2001243232A1
AU2001243232A1 AU2001243232A AU4323201A AU2001243232A1 AU 2001243232 A1 AU2001243232 A1 AU 2001243232A1 AU 2001243232 A AU2001243232 A AU 2001243232A AU 4323201 A AU4323201 A AU 4323201A AU 2001243232 A1 AU2001243232 A1 AU 2001243232A1
Authority
AU
Australia
Prior art keywords
topoisomerase
linker
amplification methods
mediated amplification
mediated
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001243232A
Inventor
Michael Felder
John Heyman
Gabor Szalai
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
South Carolina Research Foundation (SCRF)
Original Assignee
Invitrogen Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Invitrogen Corp filed Critical Invitrogen Corp
Publication of AU2001243232A1 publication Critical patent/AU2001243232A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855Ligating adaptors

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2001243232A 2000-02-25 2001-02-23 Topoisomerase linker-mediated amplification methods Abandoned AU2001243232A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US18485800P 2000-02-25 2000-02-25
US60184858 2000-02-25
PCT/US2001/005745 WO2001062943A1 (en) 2000-02-25 2001-02-23 Topoisomerase linker-mediated amplification methods

Publications (1)

Publication Number Publication Date
AU2001243232A1 true AU2001243232A1 (en) 2001-09-03

Family

ID=22678649

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001243232A Abandoned AU2001243232A1 (en) 2000-02-25 2001-02-23 Topoisomerase linker-mediated amplification methods

Country Status (5)

Country Link
US (1) US7078501B2 (en)
EP (1) EP1261724A4 (en)
JP (1) JP2003524422A (en)
AU (1) AU2001243232A1 (en)
WO (1) WO2001062943A1 (en)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5766891A (en) 1994-12-19 1998-06-16 Sloan-Kettering Institute For Cancer Research Method for molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase
DE69827745T2 (en) 1997-06-12 2005-11-10 Sloan-Kettering Institute For Cancer Research COVALENT BINDING OF DNA TO RNA STRANDS CATALYSED BY VACCINIA TOPOISOMERASE
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US6916632B2 (en) 2000-08-21 2005-07-12 Invitrogen Corporation Methods and reagents for molecular cloning
US20060008817A1 (en) * 2000-12-08 2006-01-12 Invitrogen Corporation Methods and compositions for generating recombinant nucleic acid molecules
AU2002248173B2 (en) * 2000-12-08 2007-04-26 Invitrogen Corporation Compositions and methods for rapidly generating recombinant nucleic acid molecules
WO2005054438A2 (en) 2003-12-01 2005-06-16 Invitrogen Corporation Nucleic acid molecules containing recombination sites and methods of using the same
US20060063184A1 (en) * 2004-09-09 2006-03-23 Felix Carolyn A Compositions and methods for the detection of DNA topoisomerase II complexes with DNA
BR112014025157B1 (en) 2012-04-10 2022-02-08 Oxford Nanopore Technologies Limited MUTANT LYSENINE MONOMER, CONSTRUCT, PORE, METHOD FOR CHARACTERIZING A TARGET ANALYTE, USE OF A PORE, AND, KIT
WO2014135838A1 (en) 2013-03-08 2014-09-12 Oxford Nanopore Technologies Limited Enzyme stalling method
GB201313477D0 (en) 2013-07-29 2013-09-11 Univ Leuven Kath Nanopore biosensors for detection of proteins and nucleic acids
AU2015208919B9 (en) * 2014-01-22 2021-04-01 Oxford Nanopore Technologies Limited Method for attaching one or more polynucleotide binding proteins to a target polynucleotide
WO2015166275A1 (en) 2014-05-02 2015-11-05 Oxford Nanopore Technologies Limited Mutant pores
US11274333B2 (en) 2015-05-29 2022-03-15 Molecular Cloning Laboratories (MCLAB) LLC Compositions and methods for preparing sequencing libraries
EP3103885B1 (en) * 2015-06-09 2019-01-30 Centrillion Technology Holdings Corporation Methods for sequencing nucleic acids
EP4019543A1 (en) 2016-03-02 2022-06-29 Oxford Nanopore Technologies plc Mutant pore
US11104709B2 (en) 2016-04-06 2021-08-31 Oxford Nanopore Technologies Ltd. Mutant pore
CA3066715A1 (en) 2017-06-30 2019-01-03 Vib Vzw Novel protein pores

Family Cites Families (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4661450A (en) 1983-05-03 1987-04-28 Molecular Genetics Research And Development Limited Partnership Molecular cloning of RNA using RNA ligase and synthetic oligonucleotides
EP0178314A1 (en) 1984-04-26 1986-04-23 The Australian National University An improved method of cloning double-stranded rna, and its use in the production of viral gene clones
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
CA2005252C (en) 1988-12-13 2001-07-17 Nahum Sonenberg Isolation of full-length cdna clones and capped mrna
JP2994010B2 (en) 1989-10-31 1999-12-27 財団法人相模中央化学研究所 Cloning vector plasmid, vector primer derived from the same plasmid and preparation of cdna bank using the same primer
CA2037349C (en) 1990-03-26 2008-06-17 James G. Wetmur Branch migration of nucleotides
US5162209A (en) * 1991-01-18 1992-11-10 Beth Israel Hospital Association Synthesis of full-length, double-stranded dna from a single-stranded linear dna template
JP3337748B2 (en) 1992-09-25 2002-10-21 財団法人神奈川科学技術アカデミー Method for synthesizing full-length cDNA, method for producing intermediate thereof, and method for producing recombinant vector containing full-length cDNA
DE4319468C1 (en) 1993-06-11 1994-11-17 Mueller Manfred W Method for producing a deoxyribonucleic acid (DNA) strand (cDNA strand) complementary to a ribonucleic acid (RNA) molecule, and the use of the method for the analysis of RNA molecules
US5766891A (en) 1994-12-19 1998-06-16 Sloan-Kettering Institute For Cancer Research Method for molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase
FR2733762B1 (en) 1995-05-02 1997-08-01 Genset Sa METHOD FOR THE SPECIFIC COUPLING OF THE HAIR OF THE 5 'END OF A RNAM FRAGMENT AND PREPARATION OF RNAM AND COMPLETE DNA
US6537776B1 (en) 1999-06-14 2003-03-25 Diversa Corporation Synthetic ligation reassembly in directed evolution
US6238884B1 (en) * 1995-12-07 2001-05-29 Diversa Corporation End selection in directed evolution
US5962271A (en) 1996-01-03 1999-10-05 Cloutech Laboratories, Inc. Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end
US6013440A (en) * 1996-03-11 2000-01-11 Affymetrix, Inc. Nucleic acid affinity columns
JP3346976B2 (en) * 1996-03-18 2002-11-18 理化学研究所 How to make a full-length cDNA library
US6495318B2 (en) 1996-06-17 2002-12-17 Vectorobjects, Llc Method and kits for preparing multicomponent nucleic acid constructs
AU3399797A (en) 1996-06-17 1998-01-07 Biodynamics Associates Method and kits for preparing multicomponent nucleic acid constructs
US5746997A (en) * 1996-10-02 1998-05-05 Epoch Pharmaceuticals, Inc. Radiohalogenation of oligonucleotides via trialkylstannylaryl conjugates
JP3441899B2 (en) 1996-11-01 2003-09-02 理化学研究所 How to make a full-length cDNA library
US5942422A (en) 1996-11-14 1999-08-24 The Trustees Of Columbia University In The City Of New York Method for generating a directed, recombinant fusion nucleic acid
US5851808A (en) * 1997-02-28 1998-12-22 Baylor College Of Medicine Rapid subcloning using site-specific recombination
US5981182A (en) * 1997-03-13 1999-11-09 Albert Einstein College Of Medicine Of Yeshiva University Vector constructs for the selection and identification of open reading frames
US6187544B1 (en) 1997-06-04 2001-02-13 Smithkline Beecham Corporation Methods for rapid cloning for full length cDNAs using a pooling strategy
DE69827745T2 (en) 1997-06-12 2005-11-10 Sloan-Kettering Institute For Cancer Research COVALENT BINDING OF DNA TO RNA STRANDS CATALYSED BY VACCINIA TOPOISOMERASE
US6140086A (en) 1997-08-15 2000-10-31 Fox; Donna K. Methods and compositions for cloning nucleic acid molecules
JP4304348B2 (en) 1997-09-22 2009-07-29 独立行政法人理化学研究所 DNA isolation method
US6413776B1 (en) 1998-06-12 2002-07-02 Galapagos Geonomics N.V. High throughput screening of gene function using adenoviral libraries for functional genomics applications
AU5584999A (en) 1998-08-28 2000-03-21 Invitrogen Corporation System for the rapid manipulation of nucleic acid sequences
CA2367723A1 (en) 1999-03-19 2000-09-28 Invitrogen Corporation Methods of obtaining full-length nucleic acid sequences using e. coli topoisomerase iii and its homologs
JP2002539800A (en) 1999-03-24 2002-11-26 ジーン セラピー システムズ インコーポレーテッド Method for producing transcriptionally active DNA fragment
JP2004503207A (en) 2000-02-25 2004-02-05 ストラタジーン Methods for ligating nucleic acids and molecular cloning
US20020025561A1 (en) 2000-04-17 2002-02-28 Hodgson Clague Pitman Vectors for gene-self-assembly
US20020068290A1 (en) 2000-05-31 2002-06-06 Timur Yarovinsky Topoisomerase activated oligonucleotide adaptors and uses therefor
US6916632B2 (en) 2000-08-21 2005-07-12 Invitrogen Corporation Methods and reagents for molecular cloning
AU2002248173B2 (en) 2000-12-08 2007-04-26 Invitrogen Corporation Compositions and methods for rapidly generating recombinant nucleic acid molecules

Also Published As

Publication number Publication date
JP2003524422A (en) 2003-08-19
US20010044137A1 (en) 2001-11-22
EP1261724A1 (en) 2002-12-04
US7078501B2 (en) 2006-07-18
EP1261724A4 (en) 2004-08-18
WO2001062943A1 (en) 2001-08-30

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