AU2001243232A1 - Topoisomerase linker-mediated amplification methods - Google Patents
Topoisomerase linker-mediated amplification methodsInfo
- Publication number
- AU2001243232A1 AU2001243232A1 AU2001243232A AU4323201A AU2001243232A1 AU 2001243232 A1 AU2001243232 A1 AU 2001243232A1 AU 2001243232 A AU2001243232 A AU 2001243232A AU 4323201 A AU4323201 A AU 4323201A AU 2001243232 A1 AU2001243232 A1 AU 2001243232A1
- Authority
- AU
- Australia
- Prior art keywords
- topoisomerase
- linker
- amplification methods
- mediated amplification
- mediated
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
- C12Q1/6855—Ligating adaptors
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Biomedical Technology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US18485800P | 2000-02-25 | 2000-02-25 | |
US60184858 | 2000-02-25 | ||
PCT/US2001/005745 WO2001062943A1 (en) | 2000-02-25 | 2001-02-23 | Topoisomerase linker-mediated amplification methods |
Publications (1)
Publication Number | Publication Date |
---|---|
AU2001243232A1 true AU2001243232A1 (en) | 2001-09-03 |
Family
ID=22678649
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AU2001243232A Abandoned AU2001243232A1 (en) | 2000-02-25 | 2001-02-23 | Topoisomerase linker-mediated amplification methods |
Country Status (5)
Country | Link |
---|---|
US (1) | US7078501B2 (en) |
EP (1) | EP1261724A4 (en) |
JP (1) | JP2003524422A (en) |
AU (1) | AU2001243232A1 (en) |
WO (1) | WO2001062943A1 (en) |
Families Citing this family (18)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5766891A (en) | 1994-12-19 | 1998-06-16 | Sloan-Kettering Institute For Cancer Research | Method for molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase |
DE69827745T2 (en) | 1997-06-12 | 2005-11-10 | Sloan-Kettering Institute For Cancer Research | COVALENT BINDING OF DNA TO RNA STRANDS CATALYSED BY VACCINIA TOPOISOMERASE |
US7198924B2 (en) | 2000-12-11 | 2007-04-03 | Invitrogen Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
US6916632B2 (en) | 2000-08-21 | 2005-07-12 | Invitrogen Corporation | Methods and reagents for molecular cloning |
US20060008817A1 (en) * | 2000-12-08 | 2006-01-12 | Invitrogen Corporation | Methods and compositions for generating recombinant nucleic acid molecules |
AU2002248173B2 (en) * | 2000-12-08 | 2007-04-26 | Invitrogen Corporation | Compositions and methods for rapidly generating recombinant nucleic acid molecules |
WO2005054438A2 (en) | 2003-12-01 | 2005-06-16 | Invitrogen Corporation | Nucleic acid molecules containing recombination sites and methods of using the same |
US20060063184A1 (en) * | 2004-09-09 | 2006-03-23 | Felix Carolyn A | Compositions and methods for the detection of DNA topoisomerase II complexes with DNA |
BR112014025157B1 (en) | 2012-04-10 | 2022-02-08 | Oxford Nanopore Technologies Limited | MUTANT LYSENINE MONOMER, CONSTRUCT, PORE, METHOD FOR CHARACTERIZING A TARGET ANALYTE, USE OF A PORE, AND, KIT |
WO2014135838A1 (en) | 2013-03-08 | 2014-09-12 | Oxford Nanopore Technologies Limited | Enzyme stalling method |
GB201313477D0 (en) | 2013-07-29 | 2013-09-11 | Univ Leuven Kath | Nanopore biosensors for detection of proteins and nucleic acids |
AU2015208919B9 (en) * | 2014-01-22 | 2021-04-01 | Oxford Nanopore Technologies Limited | Method for attaching one or more polynucleotide binding proteins to a target polynucleotide |
WO2015166275A1 (en) | 2014-05-02 | 2015-11-05 | Oxford Nanopore Technologies Limited | Mutant pores |
US11274333B2 (en) | 2015-05-29 | 2022-03-15 | Molecular Cloning Laboratories (MCLAB) LLC | Compositions and methods for preparing sequencing libraries |
EP3103885B1 (en) * | 2015-06-09 | 2019-01-30 | Centrillion Technology Holdings Corporation | Methods for sequencing nucleic acids |
EP4019543A1 (en) | 2016-03-02 | 2022-06-29 | Oxford Nanopore Technologies plc | Mutant pore |
US11104709B2 (en) | 2016-04-06 | 2021-08-31 | Oxford Nanopore Technologies Ltd. | Mutant pore |
CA3066715A1 (en) | 2017-06-30 | 2019-01-03 | Vib Vzw | Novel protein pores |
Family Cites Families (36)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4661450A (en) | 1983-05-03 | 1987-04-28 | Molecular Genetics Research And Development Limited Partnership | Molecular cloning of RNA using RNA ligase and synthetic oligonucleotides |
EP0178314A1 (en) | 1984-04-26 | 1986-04-23 | The Australian National University | An improved method of cloning double-stranded rna, and its use in the production of viral gene clones |
US4800159A (en) | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
CA2005252C (en) | 1988-12-13 | 2001-07-17 | Nahum Sonenberg | Isolation of full-length cdna clones and capped mrna |
JP2994010B2 (en) | 1989-10-31 | 1999-12-27 | 財団法人相模中央化学研究所 | Cloning vector plasmid, vector primer derived from the same plasmid and preparation of cdna bank using the same primer |
CA2037349C (en) | 1990-03-26 | 2008-06-17 | James G. Wetmur | Branch migration of nucleotides |
US5162209A (en) * | 1991-01-18 | 1992-11-10 | Beth Israel Hospital Association | Synthesis of full-length, double-stranded dna from a single-stranded linear dna template |
JP3337748B2 (en) | 1992-09-25 | 2002-10-21 | 財団法人神奈川科学技術アカデミー | Method for synthesizing full-length cDNA, method for producing intermediate thereof, and method for producing recombinant vector containing full-length cDNA |
DE4319468C1 (en) | 1993-06-11 | 1994-11-17 | Mueller Manfred W | Method for producing a deoxyribonucleic acid (DNA) strand (cDNA strand) complementary to a ribonucleic acid (RNA) molecule, and the use of the method for the analysis of RNA molecules |
US5766891A (en) | 1994-12-19 | 1998-06-16 | Sloan-Kettering Institute For Cancer Research | Method for molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase |
FR2733762B1 (en) | 1995-05-02 | 1997-08-01 | Genset Sa | METHOD FOR THE SPECIFIC COUPLING OF THE HAIR OF THE 5 'END OF A RNAM FRAGMENT AND PREPARATION OF RNAM AND COMPLETE DNA |
US6537776B1 (en) | 1999-06-14 | 2003-03-25 | Diversa Corporation | Synthetic ligation reassembly in directed evolution |
US6238884B1 (en) * | 1995-12-07 | 2001-05-29 | Diversa Corporation | End selection in directed evolution |
US5962271A (en) | 1996-01-03 | 1999-10-05 | Cloutech Laboratories, Inc. | Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end |
US6013440A (en) * | 1996-03-11 | 2000-01-11 | Affymetrix, Inc. | Nucleic acid affinity columns |
JP3346976B2 (en) * | 1996-03-18 | 2002-11-18 | 理化学研究所 | How to make a full-length cDNA library |
US6495318B2 (en) | 1996-06-17 | 2002-12-17 | Vectorobjects, Llc | Method and kits for preparing multicomponent nucleic acid constructs |
AU3399797A (en) | 1996-06-17 | 1998-01-07 | Biodynamics Associates | Method and kits for preparing multicomponent nucleic acid constructs |
US5746997A (en) * | 1996-10-02 | 1998-05-05 | Epoch Pharmaceuticals, Inc. | Radiohalogenation of oligonucleotides via trialkylstannylaryl conjugates |
JP3441899B2 (en) | 1996-11-01 | 2003-09-02 | 理化学研究所 | How to make a full-length cDNA library |
US5942422A (en) | 1996-11-14 | 1999-08-24 | The Trustees Of Columbia University In The City Of New York | Method for generating a directed, recombinant fusion nucleic acid |
US5851808A (en) * | 1997-02-28 | 1998-12-22 | Baylor College Of Medicine | Rapid subcloning using site-specific recombination |
US5981182A (en) * | 1997-03-13 | 1999-11-09 | Albert Einstein College Of Medicine Of Yeshiva University | Vector constructs for the selection and identification of open reading frames |
US6187544B1 (en) | 1997-06-04 | 2001-02-13 | Smithkline Beecham Corporation | Methods for rapid cloning for full length cDNAs using a pooling strategy |
DE69827745T2 (en) | 1997-06-12 | 2005-11-10 | Sloan-Kettering Institute For Cancer Research | COVALENT BINDING OF DNA TO RNA STRANDS CATALYSED BY VACCINIA TOPOISOMERASE |
US6140086A (en) | 1997-08-15 | 2000-10-31 | Fox; Donna K. | Methods and compositions for cloning nucleic acid molecules |
JP4304348B2 (en) | 1997-09-22 | 2009-07-29 | 独立行政法人理化学研究所 | DNA isolation method |
US6413776B1 (en) | 1998-06-12 | 2002-07-02 | Galapagos Geonomics N.V. | High throughput screening of gene function using adenoviral libraries for functional genomics applications |
AU5584999A (en) | 1998-08-28 | 2000-03-21 | Invitrogen Corporation | System for the rapid manipulation of nucleic acid sequences |
CA2367723A1 (en) | 1999-03-19 | 2000-09-28 | Invitrogen Corporation | Methods of obtaining full-length nucleic acid sequences using e. coli topoisomerase iii and its homologs |
JP2002539800A (en) | 1999-03-24 | 2002-11-26 | ジーン セラピー システムズ インコーポレーテッド | Method for producing transcriptionally active DNA fragment |
JP2004503207A (en) | 2000-02-25 | 2004-02-05 | ストラタジーン | Methods for ligating nucleic acids and molecular cloning |
US20020025561A1 (en) | 2000-04-17 | 2002-02-28 | Hodgson Clague Pitman | Vectors for gene-self-assembly |
US20020068290A1 (en) | 2000-05-31 | 2002-06-06 | Timur Yarovinsky | Topoisomerase activated oligonucleotide adaptors and uses therefor |
US6916632B2 (en) | 2000-08-21 | 2005-07-12 | Invitrogen Corporation | Methods and reagents for molecular cloning |
AU2002248173B2 (en) | 2000-12-08 | 2007-04-26 | Invitrogen Corporation | Compositions and methods for rapidly generating recombinant nucleic acid molecules |
-
2001
- 2001-02-23 AU AU2001243232A patent/AU2001243232A1/en not_active Abandoned
- 2001-02-23 JP JP2001561753A patent/JP2003524422A/en active Pending
- 2001-02-23 EP EP01916175A patent/EP1261724A4/en not_active Withdrawn
- 2001-02-23 US US09/792,875 patent/US7078501B2/en not_active Expired - Fee Related
- 2001-02-23 WO PCT/US2001/005745 patent/WO2001062943A1/en not_active Application Discontinuation
Also Published As
Publication number | Publication date |
---|---|
JP2003524422A (en) | 2003-08-19 |
US20010044137A1 (en) | 2001-11-22 |
EP1261724A1 (en) | 2002-12-04 |
US7078501B2 (en) | 2006-07-18 |
EP1261724A4 (en) | 2004-08-18 |
WO2001062943A1 (en) | 2001-08-30 |
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