PL436077A1 - Liofilizowany i gotowy do liofilizacji rekombinowany ludzki inhibitor RNaz - Google Patents
Liofilizowany i gotowy do liofilizacji rekombinowany ludzki inhibitor RNazInfo
- Publication number
- PL436077A1 PL436077A1 PL436077A PL43607720A PL436077A1 PL 436077 A1 PL436077 A1 PL 436077A1 PL 436077 A PL436077 A PL 436077A PL 43607720 A PL43607720 A PL 43607720A PL 436077 A1 PL436077 A1 PL 436077A1
- Authority
- PL
- Poland
- Prior art keywords
- rnase inhibitor
- ready
- recombinant human
- freeze
- human rnase
- Prior art date
Links
- 238000004108 freeze drying Methods 0.000 title abstract 4
- 239000003161 ribonuclease inhibitor Substances 0.000 title abstract 4
- 238000000034 method Methods 0.000 abstract 4
- 238000000338 in vitro Methods 0.000 abstract 3
- 238000011529 RT qPCR Methods 0.000 abstract 1
- 230000003321 amplification Effects 0.000 abstract 1
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 239000002299 complementary DNA Substances 0.000 abstract 1
- 238000009472 formulation Methods 0.000 abstract 1
- 238000002955 isolation Methods 0.000 abstract 1
- 239000012669 liquid formulation Substances 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 238000000746 purification Methods 0.000 abstract 1
- 238000003762 quantitative reverse transcription PCR Methods 0.000 abstract 1
- 238000003757 reverse transcription PCR Methods 0.000 abstract 1
- 229920002477 rna polymer Polymers 0.000 abstract 1
- 238000003786 synthesis reaction Methods 0.000 abstract 1
- 238000013518 transcription Methods 0.000 abstract 1
- 230000035897 transcription Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4702—Regulators; Modulating activity
- C07K14/4703—Inhibitors; Suppressors
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Gastroenterology & Hepatology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicinal Preparation (AREA)
Abstract
Przedmiotem wynalazku są liofilizowana postać i gotowa do liofilizacji formulacja rekombinowanego ludzkiego inhibitora RNaz w wariancie dzikim SEQ ID NO 1 do zastosowań w biologii molekularnej i diagnostyce in vitro. Przedmiotem wynalazku są liofilizowany wariant rekombinowanego ludzkiego inhibitora RNaz oraz proces jego wytwarzania skutkujący sproszkowaną formą inhibitora RNaz zachowującą jego funkcjonalność i zwiększającą jego stabilność. Dodatkowo opracowano dla niego dedykowaną płynną formulację gotową do procesu liofilizacji (suszenia sublimacyjnego). Przedmiotem wynalazku jest możliwość wykorzystania obu postaci opisanych powyżej do diagnostyki in vitro oraz biologii molekularnej w procesach i zestawach do izolacji i oczyszczania materiału genetycznego RNA (kwas rybonukleinowy), systemach do syntez nici cDNA oraz technik RT-PCR, RT-qPCR, RT-LAMP, a także zestawów do transkrypcji i translacji in vitro i dedykowanych rozwiązań do amplifikacji RNA.
Priority Applications (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL436077A PL436077A1 (pl) | 2020-11-25 | 2020-11-25 | Liofilizowany i gotowy do liofilizacji rekombinowany ludzki inhibitor RNaz |
| US18/035,143 US20230406894A1 (en) | 2020-11-25 | 2021-11-25 | Production and use of a freeze-dried or ready-for-lyophilization human rnase inhibitor |
| PCT/PL2021/000085 WO2022114981A1 (en) | 2020-11-25 | 2021-11-25 | Production and use of a freeze-dried or ready-for-lyophilization human rnase inhibitor |
| EP21830807.0A EP4251643A1 (en) | 2020-11-25 | 2021-11-25 | Production and use of a freeze-dried or ready-for-lyophilization human rnase inhibitor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL436077A PL436077A1 (pl) | 2020-11-25 | 2020-11-25 | Liofilizowany i gotowy do liofilizacji rekombinowany ludzki inhibitor RNaz |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| PL436077A1 true PL436077A1 (pl) | 2022-05-30 |
Family
ID=79021815
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PL436077A PL436077A1 (pl) | 2020-11-25 | 2020-11-25 | Liofilizowany i gotowy do liofilizacji rekombinowany ludzki inhibitor RNaz |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US20230406894A1 (pl) |
| EP (1) | EP4251643A1 (pl) |
| PL (1) | PL436077A1 (pl) |
| WO (1) | WO2022114981A1 (pl) |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK0422217T3 (da) * | 1989-04-24 | 1998-03-02 | Promega Corp | Human, rekombinant, placental ribonucleaseinhibitor og fremgangsmåde til produktion |
-
2020
- 2020-11-25 PL PL436077A patent/PL436077A1/pl unknown
-
2021
- 2021-11-25 WO PCT/PL2021/000085 patent/WO2022114981A1/en unknown
- 2021-11-25 EP EP21830807.0A patent/EP4251643A1/en active Pending
- 2021-11-25 US US18/035,143 patent/US20230406894A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| US20230406894A1 (en) | 2023-12-21 |
| WO2022114981A1 (en) | 2022-06-02 |
| EP4251643A1 (en) | 2023-10-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AU778474B2 (en) | Method and medicament for inhibiting the expression of a defined gene | |
| KR101984700B1 (ko) | 폴리뉴클레오티드 및 그의 용도 | |
| WO2013118878A1 (ja) | 環状ｒｎａ及びタンパク質の製造方法 | |
| US20220380734A1 (en) | Systems and methods for lung cell expansion and differentiation | |
| Sipert et al. | MicroRNA-146a and microRNA-155 show tissue-dependent expression in dental pulp, gingival and periodontal ligament fibroblasts in vitro | |
| WO2023035372A1 (zh) | 一种有限自我复制mRNA分子***、制备方法及应用 | |
| CN114685588B (zh) | 一种含开环核苷结构的起始加帽寡核苷酸引物 | |
| EP3778893A1 (en) | Method for activating p21 gene expression | |
| PL436077A1 (pl) | Liofilizowany i gotowy do liofilizacji rekombinowany ludzki inhibitor RNaz | |
| CN108823178B (zh) | 大黄素糖基转移酶蛋白FtUGT73BE5及其编码基因与应用 | |
| CN107904220B (zh) | 利用昆虫表达***生产芦丁水解酶并制备槲皮素的方法 | |
| CN102703400A (zh) | 热启动dna聚合酶及其应用 | |
| JP2024523006A (ja) | 新規なレプリカーゼサイクリング反応（ｒｃｒ） | |
| Nüesch et al. | Proteins specifically binding to the 3′ untranslated region of hepatitis A virus RNA in persistently infected cells | |
| CN102382187A (zh) | Fbxl15蛋白片段及其编码基因和应用 | |
| CN104962561A (zh) | 酶促cGAMP生成量检测所用RNA适配体及检测方法 | |
| Konishi et al. | Molecular cloning and multifunctional characterization of host defense peptides from the bullfrog Harderian gland with special reference to catesbeianalectin | |
| CN108265067A (zh) | 新型鸭呼肠孤病毒l组基因的克隆方法 | |
| US20230099592A1 (en) | Novel replicase cycling reaction (rcr) | |
| Nubgan | The deubiquitylase-ubiquitin-specific protease 4 absence in HeLa cells leads to a reduction in Semliki Forest virus replication | |
| CN102875660B (zh) | 一种病毒诱导蛋白Mig1及其应用 | |
| US20240093286A1 (en) | Novel Replicase Cycling Reaction (RCR) and the Related RdRP-Binding Site Designs Thereof | |
| US20220411848A1 (en) | Novel Replicase Cycling Reaction (RCR) | |
| Koyama et al. | Molecular cloning and characterization of cDNAs encoding biosynthetic precursors for the antimicrobial peptides japonicin-1Ja, japonicin-2Ja, and temporin-1Ja in the Japanese brown frog, Rana japonica | |
| CN107418961A (zh) | 家蝇酚氧化酶原激活酶1基因及其重组蛋白与应用 |