PL3635101T3 - Poddane inżynierii warianty ligazy - Google Patents

Poddane inżynierii warianty ligazy

Info

Publication number
PL3635101T3
PL3635101T3 PL18798770T PL18798770T PL3635101T3 PL 3635101 T3 PL3635101 T3 PL 3635101T3 PL 18798770 T PL18798770 T PL 18798770T PL 18798770 T PL18798770 T PL 18798770T PL 3635101 T3 PL3635101 T3 PL 3635101T3
Authority
PL
Poland
Prior art keywords
engineered ligase
ligase variants
variants
engineered
ligase
Prior art date
Application number
PL18798770T
Other languages
English (en)
Inventor
Mathew G. Miller
Jonathan VROOM
Nikki DELLAS
Donald S. Baskerville
Sandy M. Gomes
David ELGART
Judy Victoria Antonio Viduya
Original Assignee
Codexis, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Codexis, Inc. filed Critical Codexis, Inc.
Publication of PL3635101T3 publication Critical patent/PL3635101T3/pl

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y605/00—Ligases forming phosphoric ester bonds (6.5)
    • C12Y605/01—Ligases forming phosphoric ester bonds (6.5) forming phosphoric ester bonds (6.5.1)
    • C12Y605/01001—DNA ligase (ATP) (6.5.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/501—Ligase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/122—Massive parallel sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
PL18798770T 2017-05-08 2018-05-07 Poddane inżynierii warianty ligazy PL3635101T3 (pl)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201762503075P 2017-05-08 2017-05-08
US201762540734P 2017-08-03 2017-08-03
US201762587030P 2017-11-16 2017-11-16
EP18798770.6A EP3635101B1 (en) 2017-05-08 2018-05-07 Engineered ligase variants
PCT/US2018/031376 WO2018208665A1 (en) 2017-05-08 2018-05-07 Engineered ligase variants

Publications (1)

Publication Number Publication Date
PL3635101T3 true PL3635101T3 (pl) 2021-12-13

Family

ID=64014642

Family Applications (1)

Application Number Title Priority Date Filing Date
PL18798770T PL3635101T3 (pl) 2017-05-08 2018-05-07 Poddane inżynierii warianty ligazy

Country Status (15)

Country Link
US (1) US20180320162A1 (pl)
EP (2) EP3957723A3 (pl)
JP (2) JP7401902B2 (pl)
KR (1) KR20200003903A (pl)
CN (1) CN110914415A (pl)
AU (1) AU2018266606A1 (pl)
BR (1) BR112019023337A2 (pl)
CA (1) CA3062550A1 (pl)
DK (1) DK3635101T3 (pl)
ES (1) ES2887078T3 (pl)
HU (1) HUE055411T2 (pl)
MX (1) MX2019013289A (pl)
PL (1) PL3635101T3 (pl)
SG (1) SG11201909957TA (pl)
WO (1) WO2018208665A1 (pl)

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US10837009B1 (en) 2017-12-22 2020-11-17 New England Biolabs, Inc. DNA ligase variants
SG11202107418SA (en) 2019-01-06 2021-08-30 10X Genomics Inc Methods and systems for enrichment of barcodes
EP3947722A1 (en) 2019-03-27 2022-02-09 10X Genomics, Inc. Systems and methods for processing rna from cells
CN113774032B (zh) * 2021-11-12 2022-03-01 翌圣生物科技(上海)股份有限公司 重组t4连接酶突变体、编码dna及ngs建库方法
US11685913B1 (en) * 2022-02-18 2023-06-27 Abclonal Science, Inc. T4 DNA ligase variants with increased resistance to salt
US11788082B2 (en) * 2022-02-22 2023-10-17 Abclonal Science, Inc. T4 DNA ligase variants with increased thermostability
US20230295707A1 (en) * 2022-03-21 2023-09-21 Abclonal Science, Inc. T4 DNA Ligase Variants with Increased Ligation Efficiency
WO2024123733A1 (en) * 2022-12-05 2024-06-13 Twist Bioscience Corporation Enzymes for library preparation
CN115896047B (zh) * 2022-12-12 2023-06-16 南京诺唯赞生物科技股份有限公司 重组t4 dna连接酶突变体、融合蛋白及其应用
CN116218953A (zh) * 2023-01-03 2023-06-06 南京诺唯赞生物科技股份有限公司 一种连接核酸片段与接头的方法
CN117946985B (zh) * 2024-03-27 2024-05-28 翌圣生物科技(上海)股份有限公司 突变型t4 dna连接酶、试剂盒及其在文库构建中的应用

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Also Published As

Publication number Publication date
ES2887078T3 (es) 2021-12-21
KR20200003903A (ko) 2020-01-10
DK3635101T3 (da) 2021-09-20
EP3635101B1 (en) 2021-08-18
WO2018208665A1 (en) 2018-11-15
EP3635101A1 (en) 2020-04-15
EP3957723A3 (en) 2022-04-20
HUE055411T2 (hu) 2021-11-29
BR112019023337A2 (pt) 2020-06-16
CA3062550A1 (en) 2018-11-15
US20180320162A1 (en) 2018-11-08
JP2020518278A (ja) 2020-06-25
SG11201909957TA (en) 2019-11-28
JP7401902B2 (ja) 2023-12-20
CN110914415A (zh) 2020-03-24
JP2023166579A (ja) 2023-11-21
MX2019013289A (es) 2020-01-15
EP3635101A4 (en) 2020-09-30
EP3957723A2 (en) 2022-02-23
AU2018266606A1 (en) 2019-11-14

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