CA3062550A1 - Engineered ligase variants - Google Patents

Engineered ligase variants Download PDF

Info

Publication number: CA3062550A1
Authority: CA; Canada
Prior art keywords: lys; leu; glu; dna; ligase
Prior art date: 2017-05-08
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Pending

Application number

CA3062550A

Other languages

English (en)

French (fr)

Inventor

Mathew G. Miller

Jonathan VROOM

Nikki DELLAS

Donald S. Baskerville

Sandy M. Gomes

David ELGART

Judy Victoria Antonio Viduya

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Codexis Inc

Original Assignee

Codexis Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2017-05-08

Filing date

2018-05-07

Publication date

2018-11-15

2018-05-07 Application filed by Codexis Inc filed Critical Codexis Inc

2018-11-15 Publication of CA3062550A1 publication Critical patent/CA3062550A1/en

Status Pending legal-status Critical Current

Links

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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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- C12P19/00—Preparation of compounds containing saccharide radicals
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- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- C12Y—ENZYMES
- C12Y605/00—Ligases forming phosphoric ester bonds (6.5)
- C12Y605/01—Ligases forming phosphoric ester bonds (6.5) forming phosphoric ester bonds (6.5.1)
- C12Y605/01001—DNA ligase (ATP) (6.5.1.1)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/50—Other enzymatic activities
- C12Q2521/501—Ligase
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/191—Modifications characterised by incorporating an adaptor
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
- C12Q2535/122—Massive parallel sequencing

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US201762587030P	2017-11-16	2017-11-16
US62/587,030		2017-11-16
PCT/US2018/031376 WO2018208665A1 (en)	2017-05-08	2018-05-07	Engineered ligase variants

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SG11201909957TA (en)	2019-11-28
ES2887078T3 (es)	2021-12-21
BR112019023337A2 (pt)	2020-06-16
WO2018208665A1 (en)	2018-11-15
PL3635101T3 (pl)	2021-12-13
JP2023166579A (ja)	2023-11-21
KR20200003903A (ko)	2020-01-10
EP3635101A1 (en)	2020-04-15
US20180320162A1 (en)	2018-11-08
CN110914415A (zh)	2020-03-24
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AU2018266606A1 (en)	2019-11-14
EP3957723A2 (en)	2022-02-23
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