NO824102L - Fremgangsmaate og vektororganisme for styrt akkumulering av klonede heterologe genprodukter i bacillus subtilis - Google Patents

Fremgangsmaate og vektororganisme for styrt akkumulering av klonede heterologe genprodukter i bacillus subtilis

Info

Publication number: NO824102L
Authority: NO; Norway
Prior art keywords: heterologous gene; subtilis; protein; regulatory signals; plasmid
Prior art date: 1981-04-20

Application number

NO824102A

Other languages

English (en)

Norwegian (no)

Inventor

Shing Chang

Original Assignee

Cetus Corp

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1981-04-20

Filing date

1982-12-07

Publication date

1982-12-07

1982-12-07 Application filed by Cetus Corp filed Critical Cetus Corp

1982-12-07 Publication of NO824102L publication Critical patent/NO824102L/no

Links

235000014469 Bacillus subtilis Nutrition 0.000 title claims abstract description 46
238000000034 method Methods 0.000 title claims abstract description 21
239000013598 vector Substances 0.000 title abstract description 8
244000063299 Bacillus subtilis Species 0.000 title description 2
238000009825 accumulation Methods 0.000 title description 2
108090000623 proteins and genes Proteins 0.000 claims abstract description 106
102000004169 proteins and genes Human genes 0.000 claims abstract description 56
239000013612 plasmid Substances 0.000 claims abstract description 42
230000001105 regulatory effect Effects 0.000 claims abstract description 17
230000002103 transcriptional effect Effects 0.000 claims abstract description 12
210000003705 ribosome Anatomy 0.000 claims abstract description 11
230000014621 translational initiation Effects 0.000 claims abstract description 11
108091081024 Start codon Proteins 0.000 claims abstract description 9
238000004519 manufacturing process Methods 0.000 claims abstract description 9
230000010076 replication Effects 0.000 claims abstract description 8
241000894006 Bacteria Species 0.000 claims abstract description 7
210000003578 bacterial chromosome Anatomy 0.000 claims abstract description 5
239000001963 growth medium Substances 0.000 claims abstract description 3
241000282414 Homo sapiens Species 0.000 claims description 24
108090000467 Interferon-beta Proteins 0.000 claims description 19
102000003996 Interferon-beta Human genes 0.000 claims description 13
238000013519 translation Methods 0.000 claims description 9
241000194108 Bacillus licheniformis Species 0.000 claims description 6
108090000204 Dipeptidase 1 Proteins 0.000 claims description 6
102000006635 beta-lactamase Human genes 0.000 claims description 6
230000008569 process Effects 0.000 claims description 4
210000000349 chromosome Anatomy 0.000 claims description 2
244000005700 microbiome Species 0.000 claims 5
230000010354 integration Effects 0.000 claims 4
230000035897 transcription Effects 0.000 claims 4
238000013518 transcription Methods 0.000 claims 4
108020005350 Initiator Codon Proteins 0.000 claims 1
238000011084 recovery Methods 0.000 claims 1
150000001413 amino acids Chemical class 0.000 abstract description 6
238000010367 cloning Methods 0.000 abstract description 4
108090000765 processed proteins & peptides Proteins 0.000 abstract description 3
108020004414 DNA Proteins 0.000 description 20
241000588724 Escherichia coli Species 0.000 description 18
239000012634 fragment Substances 0.000 description 11
239000002773 nucleotide Substances 0.000 description 8
125000003729 nucleotide group Chemical group 0.000 description 8
108020004705 Codon Proteins 0.000 description 7
108020004511 Recombinant DNA Proteins 0.000 description 6
108091008146 restriction endonucleases Proteins 0.000 description 6
108010050904 Interferons Proteins 0.000 description 5
230000002068 genetic effect Effects 0.000 description 5
101150019841 penP gene Proteins 0.000 description 5
241000193830 Bacillus <bacterium> Species 0.000 description 4
108091026890 Coding region Proteins 0.000 description 4
108091028043 Nucleic acid sequence Proteins 0.000 description 4
108010087702 Penicillinase Proteins 0.000 description 4
240000004808 Saccharomyces cerevisiae Species 0.000 description 4
238000004458 analytical method Methods 0.000 description 4
108020004999 messenger RNA Proteins 0.000 description 4
241000206602 Eukaryota Species 0.000 description 3
241000282412 Homo Species 0.000 description 3
241001465754 Metazoa Species 0.000 description 3
210000004027 cell Anatomy 0.000 description 3
239000013599 cloning vector Substances 0.000 description 3
239000002299 complementary DNA Substances 0.000 description 3
238000010586 diagram Methods 0.000 description 3
201000010099 disease Diseases 0.000 description 3
208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
230000003834 intracellular effect Effects 0.000 description 3
229950009506 penicillinase Drugs 0.000 description 3
241000894007 species Species 0.000 description 3
206010010356 Congenital anomaly Diseases 0.000 description 2
102000053602 DNA Human genes 0.000 description 2
102000004594 DNA Polymerase I Human genes 0.000 description 2
108010017826 DNA Polymerase I Proteins 0.000 description 2
108010014303 DNA-directed DNA polymerase Proteins 0.000 description 2
102000016928 DNA-directed DNA polymerase Human genes 0.000 description 2
102000014150 Interferons Human genes 0.000 description 2
238000012300 Sequence Analysis Methods 0.000 description 2
230000000840 anti-viral effect Effects 0.000 description 2
230000000295 complement effect Effects 0.000 description 2
238000005520 cutting process Methods 0.000 description 2
238000005516 engineering process Methods 0.000 description 2
238000009585 enzyme analysis Methods 0.000 description 2
229920001519 homopolymer Polymers 0.000 description 2
229940079322 interferon Drugs 0.000 description 2
238000012986 modification Methods 0.000 description 2
230000004048 modification Effects 0.000 description 2
210000004940 nucleus Anatomy 0.000 description 2
108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
108020004635 Complementary DNA Proteins 0.000 description 1
108010008286 DNA nucleotidylexotransferase Proteins 0.000 description 1
102100029764 DNA-directed DNA/RNA polymerase mu Human genes 0.000 description 1
241000196324 Embryophyta Species 0.000 description 1
102000004190 Enzymes Human genes 0.000 description 1
108090000790 Enzymes Proteins 0.000 description 1
108060002716 Exonuclease Proteins 0.000 description 1
102100034343 Integrase Human genes 0.000 description 1
102000003960 Ligases Human genes 0.000 description 1
108090000364 Ligases Proteins 0.000 description 1
101710163270 Nuclease Proteins 0.000 description 1
108091034117 Oligonucleotide Proteins 0.000 description 1
108010092799 RNA-directed DNA polymerase Proteins 0.000 description 1
108020005038 Terminator Codon Proteins 0.000 description 1
241000700605 Viruses Species 0.000 description 1
241000607479 Yersinia pestis Species 0.000 description 1
230000009471 action Effects 0.000 description 1
230000001580 bacterial effect Effects 0.000 description 1
230000001588 bifunctional effect Effects 0.000 description 1
230000015572 biosynthetic process Effects 0.000 description 1
230000008859 change Effects 0.000 description 1
RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 1
238000000151 deposition Methods 0.000 description 1
102000013165 exonuclease Human genes 0.000 description 1
238000000855 fermentation Methods 0.000 description 1
230000004151 fermentation Effects 0.000 description 1
238000010353 genetic engineering Methods 0.000 description 1
238000009396 hybridization Methods 0.000 description 1
238000000338 in vitro Methods 0.000 description 1
230000000977 initiatory effect Effects 0.000 description 1
238000003780 insertion Methods 0.000 description 1
230000037431 insertion Effects 0.000 description 1
210000000936 intestine Anatomy 0.000 description 1
238000002955 isolation Methods 0.000 description 1
238000013507 mapping Methods 0.000 description 1
230000007246 mechanism Effects 0.000 description 1
230000001404 mediated effect Effects 0.000 description 1
229930182817 methionine Natural products 0.000 description 1
125000001360 methionine group Chemical group N[C@@H](CCSC)C(=O)* 0.000 description 1
210000000633 nuclear envelope Anatomy 0.000 description 1
230000001717 pathogenic effect Effects 0.000 description 1
210000001236 prokaryotic cell Anatomy 0.000 description 1
230000008439 repair process Effects 0.000 description 1
230000003362 replicative effect Effects 0.000 description 1
238000000926 separation method Methods 0.000 description 1
125000006850 spacer group Chemical group 0.000 description 1
239000000758 substrate Substances 0.000 description 1
238000003786 synthesis reaction Methods 0.000 description 1
230000009466 transformation Effects 0.000 description 1
241001515965 unidentified phage Species 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/555—Interferons [IFN]
- C07K14/565—IFN-beta
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Zoology (AREA)
Engineering & Computer Science (AREA)
Biophysics (AREA)
Biochemistry (AREA)
Molecular Biology (AREA)
General Health & Medical Sciences (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
Wood Science & Technology (AREA)
Biotechnology (AREA)
Biomedical Technology (AREA)
Microbiology (AREA)
Plant Pathology (AREA)
Physics & Mathematics (AREA)
Toxicology (AREA)
Gastroenterology & Hepatology (AREA)
Medicinal Chemistry (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Medicines Containing Material From Animals Or Micro-Organisms (AREA)

NO824102A 1981-04-20 1982-12-07 Fremgangsmaate og vektororganisme for styrt akkumulering av klonede heterologe genprodukter i bacillus subtilis NO824102L (no)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
US25580481A	1981-04-20	1981-04-20

Publications (1)

Publication Number	Publication Date
NO824102L true NO824102L (no)	1982-12-07

Family

ID=22969947

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
NO824102A NO824102L (no)	1981-04-20	1982-12-07	Fremgangsmaate og vektororganisme for styrt akkumulering av klonede heterologe genprodukter i bacillus subtilis

Country Status (12)

Country	Link
EP (1)	EP0063494B1 (ja)
JP (1)	JPS58500590A (ja)
AT (1)	ATE48639T1 (ja)
AU (1)	AU551529B2 (ja)
CA (1)	CA1204681A (ja)
DE (1)	DE3280060D1 (ja)
DK (1)	DK563082A (ja)
ES (2)	ES8308586A1 (ja)
FI (1)	FI824238A0 (ja)
IL (1)	IL65558A0 (ja)
NO (1)	NO824102L (ja)
WO (1)	WO1982003633A1 (ja)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
PT74811B (en) *	1981-04-29	1983-10-26	Biogen Nv	Bacillus cloning vectors recombinant dna molecules bacillus hosts transformed with them and methods for expressing foreign dna sequences and producing polypeptides coded thereby
US4559300A (en) *	1983-01-18	1985-12-17	Eli Lilly And Company	Method for using an homologous bacillus promoter and associated natural or modified ribosome binding site-containing DNA sequence in streptomyces
GR81701B (ja) *	1983-01-18	1984-12-12	Lilly Co Eli
US4595660A (en) *	1983-03-02	1986-06-17	University Of Delaware	Molecular cloning with bifunctional plasmid vectors in Bacillus subtilis, mutants and substantially stably transformed mutants of Bacillus subtilis, and methods for utilizing the transformed mutants
US4585739A (en) *	1983-03-07	1986-04-29	E. I. Du Pont De Nemours And Company	Plasmid for foreign gene expression in B. subtilis
US4663280A (en) *	1983-05-19	1987-05-05	Public Health Research Institute Of The City Of New York	Expression and secretion vectors and method of constructing vectors
US5310675A (en) *	1983-06-24	1994-05-10	Genencor, Inc.	Procaryotic carbonyl hydrolases
JPS60137291A (ja) *	1983-12-26	1985-07-20	Takeda Chem Ind Ltd	発現ベクター
WO1986005812A1 (en) *	1985-03-29	1986-10-09	Biotechnica International, Inc.	Secretion vector
IT1186750B (it) *	1985-07-10	1987-12-16	Eniricerche Spa	Vettore di clonaggio,molecole di dna ricombinante,ceppi di bacillus subtilis trasformati con dette molecole e metodi per l'espressione di geni eterologhi e produzione e secrezione di proteine codificate da detti geni

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DE2961658D1 (en) *	1978-05-17	1982-02-18	Thomae Gmbh Dr K	Process for preparing human interferon
JPS5564799A (en) *	1978-11-07	1980-05-15	Toray Ind Inc	Multi-stage concentration and purification of interferon originated from human fibroblast
NL8000127A (nl) *	1979-01-15	1980-07-17	Harvard College	Werkwijze voor het vormen van prokaryotische of eukaryotische proteinen alsmede een daarbij toepasbaar gekoppeld gen.
US4311639A (en) *	1980-07-25	1982-01-19	E. I. Du Pont De Nemours And Company	Immunogenic interferon peptides

1982
- 1982-04-16 CA CA000401142A patent/CA1204681A/en not_active Expired
- 1982-04-19 ES ES511510A patent/ES8308586A1/es not_active Expired
- 1982-04-20 WO PCT/US1982/000490 patent/WO1982003633A1/en active Application Filing
- 1982-04-20 AU AU85213/82A patent/AU551529B2/en not_active Ceased
- 1982-04-20 EP EP82302027A patent/EP0063494B1/en not_active Expired
- 1982-04-20 JP JP57501695A patent/JPS58500590A/ja active Pending
- 1982-04-20 IL IL65558A patent/IL65558A0/xx unknown
- 1982-04-20 AT AT82302027T patent/ATE48639T1/de not_active IP Right Cessation
- 1982-04-20 DE DE8282302027T patent/DE3280060D1/de not_active Expired - Lifetime
- 1982-12-07 NO NO824102A patent/NO824102L/no unknown
- 1982-12-09 FI FI824238A patent/FI824238A0/fi not_active Application Discontinuation
- 1982-12-20 DK DK563082A patent/DK563082A/da not_active Application Discontinuation
1983
- 1983-01-25 ES ES519234A patent/ES8403158A1/es not_active Expired

Also Published As

Publication number	Publication date
ES511510A0 (es)	1983-09-16
DK563082A (da)	1982-12-20
AU551529B2 (en)	1986-05-01
WO1982003633A1 (en)	1982-10-28
ES519234A0 (es)	1984-03-01
EP0063494A3 (en)	1984-02-22
EP0063494A2 (en)	1982-10-27
ES8403158A1 (es)	1984-03-01
FI824238L (fi)	1982-12-09
ES8308586A1 (es)	1983-09-16
CA1204681A (en)	1986-05-20
IL65558A0 (en)	1982-07-30
JPS58500590A (ja)	1983-04-21
EP0063494B1 (en)	1989-12-13
DE3280060D1 (de)	1990-01-18
FI824238A0 (fi)	1982-12-09
ATE48639T1 (de)	1989-12-15
AU8521382A (en)	1982-11-04

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