ES8403158A1 - Un metodo para crear un microorganismo capaz de producir unaproteina predeterminada por medio de expresion. - Google Patents
Un metodo para crear un microorganismo capaz de producir unaproteina predeterminada por medio de expresion.Info
- Publication number
- ES8403158A1 ES8403158A1 ES519234A ES519234A ES8403158A1 ES 8403158 A1 ES8403158 A1 ES 8403158A1 ES 519234 A ES519234 A ES 519234A ES 519234 A ES519234 A ES 519234A ES 8403158 A1 ES8403158 A1 ES 8403158A1
- Authority
- ES
- Spain
- Prior art keywords
- heterologous gene
- subtilis
- microorganisms
- creation
- protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/555—Interferons [IFN]
- C07K14/565—IFN-beta
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
METODO PARA CREAR UN MICROORGANISMO CAPAZ DE PRODUCIR UNA PROTEINA PREDETERMINADA POR MEDIO DE EXPRESION.CONSISTE EN FORMAR UN PLASMIDO CAPAZ DE REPLICACION EN B. SUTILIS O CAPAZ DE INTEGRACION EN EL CROMOSOMA BACTERIANO DE B. SUBTILIS, EL CUAL TIENE UN GEN HETEROLOGO QUE CODIFICA UNA PROTEINA PREDETERMINADA CAPAZ DE EXPRESION EN B. SUBTILIS. DICHO GEN HETEROLOGO INCLUYE UNA SECUENCIA DE CODON DE INICIACION A LA TRADUCCION Y ESTA BAJO EL CONTROL DE SEÑALES OPERABLES REGULADORAS DE LA TRANSCRIPCION Y LA TRADUCCION QUE INCLUYEN OPERADOR, PROMOTOR Y SECUENCIAS DEL SITIODE UNION AL RIBOSOMA, SIENDO DICHAS SEÑALES OPERABLES REGULADORAS DE LA TRANSCRIPCION Y LA TRADUCCION ORIGINARIAS DE UNA FUENTE DISTINTA DE DICHO GEN HETEROLOGO.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US25580481A | 1981-04-20 | 1981-04-20 |
Publications (2)
Publication Number | Publication Date |
---|---|
ES519234A0 ES519234A0 (es) | 1984-03-01 |
ES8403158A1 true ES8403158A1 (es) | 1984-03-01 |
Family
ID=22969947
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES511510A Expired ES8308586A1 (es) | 1981-04-20 | 1982-04-19 | Un metodo para producir una proteina predeterminada por cul tivo de bacterias b subtilis transformadas. |
ES519234A Expired ES8403158A1 (es) | 1981-04-20 | 1983-01-25 | Un metodo para crear un microorganismo capaz de producir unaproteina predeterminada por medio de expresion. |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES511510A Expired ES8308586A1 (es) | 1981-04-20 | 1982-04-19 | Un metodo para producir una proteina predeterminada por cul tivo de bacterias b subtilis transformadas. |
Country Status (12)
Country | Link |
---|---|
EP (1) | EP0063494B1 (es) |
JP (1) | JPS58500590A (es) |
AT (1) | ATE48639T1 (es) |
AU (1) | AU551529B2 (es) |
CA (1) | CA1204681A (es) |
DE (1) | DE3280060D1 (es) |
DK (1) | DK563082A (es) |
ES (2) | ES8308586A1 (es) |
FI (1) | FI824238A0 (es) |
IL (1) | IL65558A0 (es) |
NO (1) | NO824102L (es) |
WO (1) | WO1982003633A1 (es) |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
PT74811B (en) * | 1981-04-29 | 1983-10-26 | Biogen Nv | Bacillus cloning vectors recombinant dna molecules bacillus hosts transformed with them and methods for expressing foreign dna sequences and producing polypeptides coded thereby |
US4559300A (en) * | 1983-01-18 | 1985-12-17 | Eli Lilly And Company | Method for using an homologous bacillus promoter and associated natural or modified ribosome binding site-containing DNA sequence in streptomyces |
GR81701B (es) * | 1983-01-18 | 1984-12-12 | Lilly Co Eli | |
US4595660A (en) * | 1983-03-02 | 1986-06-17 | University Of Delaware | Molecular cloning with bifunctional plasmid vectors in Bacillus subtilis, mutants and substantially stably transformed mutants of Bacillus subtilis, and methods for utilizing the transformed mutants |
US4585739A (en) * | 1983-03-07 | 1986-04-29 | E. I. Du Pont De Nemours And Company | Plasmid for foreign gene expression in B. subtilis |
US4663280A (en) * | 1983-05-19 | 1987-05-05 | Public Health Research Institute Of The City Of New York | Expression and secretion vectors and method of constructing vectors |
US5310675A (en) * | 1983-06-24 | 1994-05-10 | Genencor, Inc. | Procaryotic carbonyl hydrolases |
JPS60137291A (ja) * | 1983-12-26 | 1985-07-20 | Takeda Chem Ind Ltd | 発現ベクター |
WO1986005812A1 (en) * | 1985-03-29 | 1986-10-09 | Biotechnica International, Inc. | Secretion vector |
IT1186750B (it) * | 1985-07-10 | 1987-12-16 | Eniricerche Spa | Vettore di clonaggio,molecole di dna ricombinante,ceppi di bacillus subtilis trasformati con dette molecole e metodi per l'espressione di geni eterologhi e produzione e secrezione di proteine codificate da detti geni |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE2961658D1 (en) * | 1978-05-17 | 1982-02-18 | Thomae Gmbh Dr K | Process for preparing human interferon |
JPS5564799A (en) * | 1978-11-07 | 1980-05-15 | Toray Ind Inc | Multi-stage concentration and purification of interferon originated from human fibroblast |
NL8000127A (nl) * | 1979-01-15 | 1980-07-17 | Harvard College | Werkwijze voor het vormen van prokaryotische of eukaryotische proteinen alsmede een daarbij toepasbaar gekoppeld gen. |
US4311639A (en) * | 1980-07-25 | 1982-01-19 | E. I. Du Pont De Nemours And Company | Immunogenic interferon peptides |
-
1982
- 1982-04-16 CA CA000401142A patent/CA1204681A/en not_active Expired
- 1982-04-19 ES ES511510A patent/ES8308586A1/es not_active Expired
- 1982-04-20 WO PCT/US1982/000490 patent/WO1982003633A1/en active Application Filing
- 1982-04-20 AU AU85213/82A patent/AU551529B2/en not_active Ceased
- 1982-04-20 EP EP82302027A patent/EP0063494B1/en not_active Expired
- 1982-04-20 JP JP57501695A patent/JPS58500590A/ja active Pending
- 1982-04-20 IL IL65558A patent/IL65558A0/xx unknown
- 1982-04-20 AT AT82302027T patent/ATE48639T1/de not_active IP Right Cessation
- 1982-04-20 DE DE8282302027T patent/DE3280060D1/de not_active Expired - Lifetime
- 1982-12-07 NO NO824102A patent/NO824102L/no unknown
- 1982-12-09 FI FI824238A patent/FI824238A0/fi not_active Application Discontinuation
- 1982-12-20 DK DK563082A patent/DK563082A/da not_active Application Discontinuation
-
1983
- 1983-01-25 ES ES519234A patent/ES8403158A1/es not_active Expired
Also Published As
Publication number | Publication date |
---|---|
NO824102L (no) | 1982-12-07 |
ES511510A0 (es) | 1983-09-16 |
DK563082A (da) | 1982-12-20 |
AU551529B2 (en) | 1986-05-01 |
WO1982003633A1 (en) | 1982-10-28 |
ES519234A0 (es) | 1984-03-01 |
EP0063494A3 (en) | 1984-02-22 |
EP0063494A2 (en) | 1982-10-27 |
FI824238L (fi) | 1982-12-09 |
ES8308586A1 (es) | 1983-09-16 |
CA1204681A (en) | 1986-05-20 |
IL65558A0 (en) | 1982-07-30 |
JPS58500590A (ja) | 1983-04-21 |
EP0063494B1 (en) | 1989-12-13 |
DE3280060D1 (de) | 1990-01-18 |
FI824238A0 (fi) | 1982-12-09 |
ATE48639T1 (de) | 1989-12-15 |
AU8521382A (en) | 1982-11-04 |
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