NO824102L - Fremgangsmaate og vektororganisme for styrt akkumulering av klonede heterologe genprodukter i bacillus subtilis - Google Patents
Fremgangsmaate og vektororganisme for styrt akkumulering av klonede heterologe genprodukter i bacillus subtilisInfo
- Publication number
- NO824102L NO824102L NO824102A NO824102A NO824102L NO 824102 L NO824102 L NO 824102L NO 824102 A NO824102 A NO 824102A NO 824102 A NO824102 A NO 824102A NO 824102 L NO824102 L NO 824102L
- Authority
- NO
- Norway
- Prior art keywords
- heterologous gene
- subtilis
- protein
- regulatory signals
- plasmid
- Prior art date
Links
- 235000014469 Bacillus subtilis Nutrition 0.000 title claims abstract description 46
- 238000000034 method Methods 0.000 title claims abstract description 21
- 239000013598 vector Substances 0.000 title abstract description 8
- 244000063299 Bacillus subtilis Species 0.000 title description 2
- 238000009825 accumulation Methods 0.000 title description 2
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 106
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 56
- 239000013612 plasmid Substances 0.000 claims abstract description 42
- 230000001105 regulatory effect Effects 0.000 claims abstract description 17
- 230000002103 transcriptional effect Effects 0.000 claims abstract description 12
- 210000003705 ribosome Anatomy 0.000 claims abstract description 11
- 230000014621 translational initiation Effects 0.000 claims abstract description 11
- 108091081024 Start codon Proteins 0.000 claims abstract description 9
- 238000004519 manufacturing process Methods 0.000 claims abstract description 9
- 230000010076 replication Effects 0.000 claims abstract description 8
- 241000894006 Bacteria Species 0.000 claims abstract description 7
- 210000003578 bacterial chromosome Anatomy 0.000 claims abstract description 5
- 239000001963 growth medium Substances 0.000 claims abstract description 3
- 241000282414 Homo sapiens Species 0.000 claims description 24
- 108090000467 Interferon-beta Proteins 0.000 claims description 19
- 102000003996 Interferon-beta Human genes 0.000 claims description 13
- 238000013519 translation Methods 0.000 claims description 9
- 241000194108 Bacillus licheniformis Species 0.000 claims description 6
- 108090000204 Dipeptidase 1 Proteins 0.000 claims description 6
- 102000006635 beta-lactamase Human genes 0.000 claims description 6
- 230000008569 process Effects 0.000 claims description 4
- 210000000349 chromosome Anatomy 0.000 claims description 2
- 244000005700 microbiome Species 0.000 claims 5
- 230000010354 integration Effects 0.000 claims 4
- 230000035897 transcription Effects 0.000 claims 4
- 238000013518 transcription Methods 0.000 claims 4
- 108020005350 Initiator Codon Proteins 0.000 claims 1
- 238000011084 recovery Methods 0.000 claims 1
- 150000001413 amino acids Chemical class 0.000 abstract description 6
- 238000010367 cloning Methods 0.000 abstract description 4
- 108090000765 processed proteins & peptides Proteins 0.000 abstract description 3
- 108020004414 DNA Proteins 0.000 description 20
- 241000588724 Escherichia coli Species 0.000 description 18
- 239000012634 fragment Substances 0.000 description 11
- 239000002773 nucleotide Substances 0.000 description 8
- 125000003729 nucleotide group Chemical group 0.000 description 8
- 108020004705 Codon Proteins 0.000 description 7
- 108020004511 Recombinant DNA Proteins 0.000 description 6
- 108091008146 restriction endonucleases Proteins 0.000 description 6
- 108010050904 Interferons Proteins 0.000 description 5
- 230000002068 genetic effect Effects 0.000 description 5
- 101150019841 penP gene Proteins 0.000 description 5
- 241000193830 Bacillus <bacterium> Species 0.000 description 4
- 108091026890 Coding region Proteins 0.000 description 4
- 108091028043 Nucleic acid sequence Proteins 0.000 description 4
- 108010087702 Penicillinase Proteins 0.000 description 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 4
- 108020004999 messenger RNA Proteins 0.000 description 4
- 241000206602 Eukaryota Species 0.000 description 3
- 241000282412 Homo Species 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 239000013599 cloning vector Substances 0.000 description 3
- 239000002299 complementary DNA Substances 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 230000003834 intracellular effect Effects 0.000 description 3
- 229950009506 penicillinase Drugs 0.000 description 3
- 241000894007 species Species 0.000 description 3
- 206010010356 Congenital anomaly Diseases 0.000 description 2
- 102000053602 DNA Human genes 0.000 description 2
- 102000004594 DNA Polymerase I Human genes 0.000 description 2
- 108010017826 DNA Polymerase I Proteins 0.000 description 2
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 2
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 2
- 102000014150 Interferons Human genes 0.000 description 2
- 238000012300 Sequence Analysis Methods 0.000 description 2
- 230000000840 anti-viral effect Effects 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 238000005520 cutting process Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000009585 enzyme analysis Methods 0.000 description 2
- 229920001519 homopolymer Polymers 0.000 description 2
- 229940079322 interferon Drugs 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 210000004940 nucleus Anatomy 0.000 description 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
- 108020004635 Complementary DNA Proteins 0.000 description 1
- 108010008286 DNA nucleotidylexotransferase Proteins 0.000 description 1
- 102100029764 DNA-directed DNA/RNA polymerase mu Human genes 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 108060002716 Exonuclease Proteins 0.000 description 1
- 102100034343 Integrase Human genes 0.000 description 1
- 102000003960 Ligases Human genes 0.000 description 1
- 108090000364 Ligases Proteins 0.000 description 1
- 101710163270 Nuclease Proteins 0.000 description 1
- 108091034117 Oligonucleotide Proteins 0.000 description 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 1
- 108020005038 Terminator Codon Proteins 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000001588 bifunctional effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 1
- 238000000151 deposition Methods 0.000 description 1
- 102000013165 exonuclease Human genes 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000013507 mapping Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 125000001360 methionine group Chemical group N[C@@H](CCSC)C(=O)* 0.000 description 1
- 210000000633 nuclear envelope Anatomy 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 210000001236 prokaryotic cell Anatomy 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 230000003362 replicative effect Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 125000006850 spacer group Chemical group 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 241001515965 unidentified phage Species 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/555—Interferons [IFN]
- C07K14/565—IFN-beta
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Gastroenterology & Hepatology (AREA)
- Physics & Mathematics (AREA)
- Toxicology (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US25580481A | 1981-04-20 | 1981-04-20 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| NO824102L true NO824102L (no) | 1982-12-07 |
Family
ID=22969947
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NO824102A NO824102L (no) | 1981-04-20 | 1982-12-07 | Fremgangsmaate og vektororganisme for styrt akkumulering av klonede heterologe genprodukter i bacillus subtilis |
Country Status (12)
| Country | Link |
|---|---|
| EP (1) | EP0063494B1 (es) |
| JP (1) | JPS58500590A (es) |
| AT (1) | ATE48639T1 (es) |
| AU (1) | AU551529B2 (es) |
| CA (1) | CA1204681A (es) |
| DE (1) | DE3280060D1 (es) |
| DK (1) | DK563082A (es) |
| ES (2) | ES511510A0 (es) |
| FI (1) | FI824238L (es) |
| IL (1) | IL65558A0 (es) |
| NO (1) | NO824102L (es) |
| WO (1) | WO1982003633A1 (es) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU8300282A (en) * | 1981-04-29 | 1982-11-04 | Biogen N.V. | Bacillus cloning vectors and method of expressing foreign dna |
| US4559300A (en) * | 1983-01-18 | 1985-12-17 | Eli Lilly And Company | Method for using an homologous bacillus promoter and associated natural or modified ribosome binding site-containing DNA sequence in streptomyces |
| GR81701B (es) * | 1983-01-18 | 1984-12-12 | Lilly Co Eli | |
| US4595660A (en) * | 1983-03-02 | 1986-06-17 | University Of Delaware | Molecular cloning with bifunctional plasmid vectors in Bacillus subtilis, mutants and substantially stably transformed mutants of Bacillus subtilis, and methods for utilizing the transformed mutants |
| US4585739A (en) * | 1983-03-07 | 1986-04-29 | E. I. Du Pont De Nemours And Company | Plasmid for foreign gene expression in B. subtilis |
| US4663280A (en) * | 1983-05-19 | 1987-05-05 | Public Health Research Institute Of The City Of New York | Expression and secretion vectors and method of constructing vectors |
| US5310675A (en) * | 1983-06-24 | 1994-05-10 | Genencor, Inc. | Procaryotic carbonyl hydrolases |
| JPS60137291A (ja) * | 1983-12-26 | 1985-07-20 | Takeda Chem Ind Ltd | 発現ベクター |
| AU5668486A (en) * | 1985-03-29 | 1986-10-23 | Biotechnica International Inc. | Secretion vector |
| IT1186750B (it) * | 1985-07-10 | 1987-12-16 | Eniricerche Spa | Vettore di clonaggio,molecole di dna ricombinante,ceppi di bacillus subtilis trasformati con dette molecole e metodi per l'espressione di geni eterologhi e produzione e secrezione di proteine codificate da detti geni |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2961658D1 (en) * | 1978-05-17 | 1982-02-18 | Thomae Gmbh Dr K | Process for preparing human interferon |
| JPS5564799A (en) * | 1978-11-07 | 1980-05-15 | Toray Ind Inc | Multi-stage concentration and purification of interferon originated from human fibroblast |
| NL8000127A (nl) * | 1979-01-15 | 1980-07-17 | Harvard College | Werkwijze voor het vormen van prokaryotische of eukaryotische proteinen alsmede een daarbij toepasbaar gekoppeld gen. |
| US4311639A (en) * | 1980-07-25 | 1982-01-19 | E. I. Du Pont De Nemours And Company | Immunogenic interferon peptides |
-
1982
- 1982-04-16 CA CA000401142A patent/CA1204681A/en not_active Expired
- 1982-04-19 ES ES511510A patent/ES511510A0/es active Granted
- 1982-04-20 DE DE8282302027T patent/DE3280060D1/de not_active Expired - Lifetime
- 1982-04-20 JP JP57501695A patent/JPS58500590A/ja active Pending
- 1982-04-20 IL IL65558A patent/IL65558A0/xx unknown
- 1982-04-20 AT AT82302027T patent/ATE48639T1/de not_active IP Right Cessation
- 1982-04-20 EP EP82302027A patent/EP0063494B1/en not_active Expired
- 1982-04-20 AU AU85213/82A patent/AU551529B2/en not_active Ceased
- 1982-04-20 WO PCT/US1982/000490 patent/WO1982003633A1/en active Application Filing
- 1982-12-07 NO NO824102A patent/NO824102L/no unknown
- 1982-12-09 FI FI824238A patent/FI824238L/fi not_active Application Discontinuation
- 1982-12-20 DK DK563082A patent/DK563082A/da not_active Application Discontinuation
-
1983
- 1983-01-25 ES ES519234A patent/ES8403158A1/es not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| ES8308586A1 (es) | 1983-09-16 |
| AU8521382A (en) | 1982-11-04 |
| EP0063494B1 (en) | 1989-12-13 |
| JPS58500590A (ja) | 1983-04-21 |
| EP0063494A2 (en) | 1982-10-27 |
| FI824238A0 (fi) | 1982-12-09 |
| EP0063494A3 (en) | 1984-02-22 |
| CA1204681A (en) | 1986-05-20 |
| ATE48639T1 (de) | 1989-12-15 |
| DK563082A (da) | 1982-12-20 |
| DE3280060D1 (de) | 1990-01-18 |
| WO1982003633A1 (en) | 1982-10-28 |
| AU551529B2 (en) | 1986-05-01 |
| ES519234A0 (es) | 1984-03-01 |
| ES8403158A1 (es) | 1984-03-01 |
| FI824238L (fi) | 1982-12-09 |
| IL65558A0 (en) | 1982-07-30 |
| ES511510A0 (es) | 1983-09-16 |
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