NO790252L - Spesifikke bindings-proevings teknikker - Google Patents
Spesifikke bindings-proevings teknikkerInfo
- Publication number
- NO790252L NO790252L NO790252A NO790252A NO790252L NO 790252 L NO790252 L NO 790252L NO 790252 A NO790252 A NO 790252A NO 790252 A NO790252 A NO 790252A NO 790252 L NO790252 L NO 790252L
- Authority
- NO
- Norway
- Prior art keywords
- ige
- labeled
- ligand
- amount
- radiolabeled
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims description 97
- 230000009870 specific binding Effects 0.000 title claims description 34
- 238000012360 testing method Methods 0.000 title claims description 24
- 239000003446 ligand Substances 0.000 claims description 75
- 239000000758 substrate Substances 0.000 claims description 47
- 238000009739 binding Methods 0.000 claims description 45
- 230000027455 binding Effects 0.000 claims description 44
- 102000004169 proteins and genes Human genes 0.000 claims description 37
- 108090000623 proteins and genes Proteins 0.000 claims description 37
- 210000002966 serum Anatomy 0.000 claims description 36
- 239000000126 substance Substances 0.000 claims description 36
- 239000013566 allergen Substances 0.000 claims description 35
- 239000007790 solid phase Substances 0.000 claims description 23
- 229940074608 allergen extract Drugs 0.000 claims description 20
- 238000002372 labelling Methods 0.000 claims description 20
- 239000013558 reference substance Substances 0.000 claims description 20
- 238000000159 protein binding assay Methods 0.000 claims description 15
- 230000005764 inhibitory process Effects 0.000 claims description 10
- 238000010998 test method Methods 0.000 claims description 10
- 108090000790 Enzymes Proteins 0.000 claims description 7
- 102000004190 Enzymes Human genes 0.000 claims description 7
- 238000006243 chemical reaction Methods 0.000 claims description 7
- 238000010790 dilution Methods 0.000 claims description 6
- 239000012895 dilution Substances 0.000 claims description 6
- 230000002285 radioactive effect Effects 0.000 claims description 6
- 239000005515 coenzyme Substances 0.000 claims description 5
- 230000007423 decrease Effects 0.000 claims description 5
- 239000002532 enzyme inhibitor Substances 0.000 claims description 5
- 230000036541 health Effects 0.000 claims description 5
- 230000008520 organization Effects 0.000 claims description 5
- 241001515965 unidentified phage Species 0.000 claims description 5
- 230000006957 competitive inhibition Effects 0.000 claims description 3
- 239000012925 reference material Substances 0.000 claims 2
- 238000009738 saturating Methods 0.000 claims 2
- 241000894007 species Species 0.000 claims 1
- 239000000427 antigen Substances 0.000 description 44
- 102000036639 antigens Human genes 0.000 description 44
- 108091007433 antigens Proteins 0.000 description 44
- 206010003645 Atopy Diseases 0.000 description 17
- 239000003153 chemical reaction reagent Substances 0.000 description 13
- 230000005855 radiation Effects 0.000 description 13
- 239000000284 extract Substances 0.000 description 11
- 239000000047 product Substances 0.000 description 11
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 9
- 239000000203 mixture Substances 0.000 description 9
- 239000002953 phosphate buffered saline Substances 0.000 description 9
- 239000000243 solution Substances 0.000 description 9
- 206010020751 Hypersensitivity Diseases 0.000 description 8
- 239000000463 material Substances 0.000 description 7
- 239000008363 phosphate buffer Substances 0.000 description 7
- 238000002360 preparation method Methods 0.000 description 7
- 229960004784 allergens Drugs 0.000 description 6
- 208000026935 allergic disease Diseases 0.000 description 6
- 238000004448 titration Methods 0.000 description 6
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 5
- 230000007815 allergy Effects 0.000 description 5
- 229940098773 bovine serum albumin Drugs 0.000 description 5
- 238000011088 calibration curve Methods 0.000 description 5
- 238000002405 diagnostic procedure Methods 0.000 description 5
- 238000011534 incubation Methods 0.000 description 5
- 239000004094 surface-active agent Substances 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 210000001124 body fluid Anatomy 0.000 description 4
- 239000010839 body fluid Substances 0.000 description 4
- 229940088598 enzyme Drugs 0.000 description 4
- 238000000338 in vitro Methods 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 239000002504 physiological saline solution Substances 0.000 description 4
- 206010028980 Neoplasm Diseases 0.000 description 3
- 241001494479 Pecora Species 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 230000000172 allergic effect Effects 0.000 description 3
- 208000010668 atopic eczema Diseases 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 239000000872 buffer Substances 0.000 description 3
- 239000007795 chemical reaction product Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000012634 fragment Substances 0.000 description 3
- 238000001727 in vivo Methods 0.000 description 3
- 238000002347 injection Methods 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- 230000026045 iodination Effects 0.000 description 3
- 238000006192 iodination reaction Methods 0.000 description 3
- 239000007791 liquid phase Substances 0.000 description 3
- 238000000163 radioactive labelling Methods 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- 108700004676 Bence Jones Proteins 0.000 description 2
- 241000209210 Dactylis Species 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- 102000009438 IgE Receptors Human genes 0.000 description 2
- 108010073816 IgE Receptors Proteins 0.000 description 2
- 108060003951 Immunoglobulin Proteins 0.000 description 2
- 241000283973 Oryctolagus cuniculus Species 0.000 description 2
- 108090000526 Papain Proteins 0.000 description 2
- 239000004365 Protease Substances 0.000 description 2
- 229920005654 Sephadex Polymers 0.000 description 2
- 239000012507 Sephadex™ Substances 0.000 description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- 239000008351 acetate buffer Substances 0.000 description 2
- 150000001413 amino acids Chemical group 0.000 description 2
- 239000003708 ampul Substances 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 230000004071 biological effect Effects 0.000 description 2
- 201000011510 cancer Diseases 0.000 description 2
- VDQQXEISLMTGAB-UHFFFAOYSA-N chloramine T Chemical compound [Na+].CC1=CC=C(S(=O)(=O)[N-]Cl)C=C1 VDQQXEISLMTGAB-UHFFFAOYSA-N 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- ATDGTVJJHBUTRL-UHFFFAOYSA-N cyanogen bromide Chemical compound BrC#N ATDGTVJJHBUTRL-UHFFFAOYSA-N 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 229940046528 grass pollen Drugs 0.000 description 2
- 102000018358 immunoglobulin Human genes 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 230000010807 negative regulation of binding Effects 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 229940055729 papain Drugs 0.000 description 2
- 235000019834 papain Nutrition 0.000 description 2
- 238000003127 radioimmunoassay Methods 0.000 description 2
- 239000011541 reaction mixture Substances 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- HRZFUMHJMZEROT-UHFFFAOYSA-L sodium disulfite Chemical compound [Na+].[Na+].[O-]S(=O)S([O-])(=O)=O HRZFUMHJMZEROT-UHFFFAOYSA-L 0.000 description 2
- 229940001584 sodium metabisulfite Drugs 0.000 description 2
- 235000010262 sodium metabisulphite Nutrition 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 208000035285 Allergic Seasonal Rhinitis Diseases 0.000 description 1
- 206010002199 Anaphylactic shock Diseases 0.000 description 1
- RZZPDXZPRHQOCG-OJAKKHQRSA-O CDP-choline(1+) Chemical compound O[C@@H]1[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OCC[N+](C)(C)C)O[C@H]1N1C(=O)N=C(N)C=C1 RZZPDXZPRHQOCG-OJAKKHQRSA-O 0.000 description 1
- 241000282693 Cercopithecidae Species 0.000 description 1
- 240000004585 Dactylis glomerata Species 0.000 description 1
- 208000010201 Exanthema Diseases 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- 108091006905 Human Serum Albumin Proteins 0.000 description 1
- 102000008100 Human Serum Albumin Human genes 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 102000008394 Immunoglobulin Fragments Human genes 0.000 description 1
- 108010021625 Immunoglobulin Fragments Proteins 0.000 description 1
- 108010045503 Myeloma Proteins Proteins 0.000 description 1
- 102000005717 Myeloma Proteins Human genes 0.000 description 1
- 206010035226 Plasma cell myeloma Diseases 0.000 description 1
- 208000024780 Urticaria Diseases 0.000 description 1
- PNDPGZBMCMUPRI-XXSWNUTMSA-N [125I][125I] Chemical compound [125I][125I] PNDPGZBMCMUPRI-XXSWNUTMSA-N 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000002009 allergenic effect Effects 0.000 description 1
- 208000030961 allergic reaction Diseases 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 208000003455 anaphylaxis Diseases 0.000 description 1
- 208000006673 asthma Diseases 0.000 description 1
- 208000010216 atopic IgE responsiveness Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000010908 decantation Methods 0.000 description 1
- 238000000586 desensitisation Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 231100000673 dose–response relationship Toxicity 0.000 description 1
- 238000005530 etching Methods 0.000 description 1
- 201000005884 exanthem Diseases 0.000 description 1
- 238000005227 gel permeation chromatography Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 150000002496 iodine Chemical class 0.000 description 1
- 229940044173 iodine-125 Drugs 0.000 description 1
- 238000004255 ion exchange chromatography Methods 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 150000002540 isothiocyanates Chemical class 0.000 description 1
- 208000019420 lymphoid neoplasm Diseases 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000010907 mechanical stirring Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 201000000050 myeloid neoplasm Diseases 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 239000002420 orchard Substances 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 239000012857 radioactive material Substances 0.000 description 1
- 206010037844 rash Diseases 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000000284 resting effect Effects 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 239000001022 rhodamine dye Substances 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000013207 serial dilution Methods 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 239000012224 working solution Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6854—Immunoglobulins
- G01N33/6857—Antibody fragments
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/804—Radioisotope, e.g. radioimmunoassay
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US05/872,668 US4211762A (en) | 1978-01-26 | 1978-01-26 | Specific binding assay techniques |
Publications (1)
Publication Number | Publication Date |
---|---|
NO790252L true NO790252L (no) | 1979-07-27 |
Family
ID=25360070
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
NO790252A NO790252L (no) | 1978-01-26 | 1979-01-25 | Spesifikke bindings-proevings teknikker |
Country Status (10)
Country | Link |
---|---|
US (1) | US4211762A (de) |
AU (1) | AU513556B2 (de) |
CA (1) | CA1114290A (de) |
DE (1) | DE2900546C3 (de) |
DK (1) | DK32579A (de) |
FI (1) | FI790228A (de) |
FR (1) | FR2445966A1 (de) |
GB (1) | GB2013337B (de) |
NO (1) | NO790252L (de) |
SE (1) | SE7900343L (de) |
Families Citing this family (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE2953610C1 (de) * | 1979-03-19 | 1985-04-11 | International Diagnostic Technology, Inc., Santa Clara, Calif. | Verfahren zum Bestimmen eines immunologisch aktiven Stoffs |
DE3168691D1 (en) * | 1980-11-07 | 1985-03-14 | Technicon Instr | Immunoassay of proteins |
US4444879A (en) * | 1981-01-29 | 1984-04-24 | Science Research Center, Inc. | Immunoassay with article having support film and immunological counterpart of analyte |
FR2502786B1 (fr) * | 1981-03-24 | 1985-06-21 | Stallergenes Laboratoire | Procede de fixation d'antigenes et d'anticorps sur support de polysaccharides, et utilisation du produit ainsi obtenu pour les dosages immunologiques |
FR2503578B1 (fr) * | 1981-04-13 | 1987-07-17 | Guinard Separation | Installation de filtration a coursiers passant entre des plateaux de serrage |
AU580248B2 (en) * | 1982-10-13 | 1989-01-12 | Biowhittaker Technologies, Inc. | Fluorometric assay of allergic reactions and reagents therefor |
US4845027B1 (en) * | 1982-10-13 | 1994-05-10 | Biowhittaker Inc | Fluorometric assay of allergic reactions |
US4528267A (en) * | 1982-11-26 | 1985-07-09 | Axionics, Inc. | Fluorometirc enzyme inhibition immunoassay for measuring potency of allergen extracts |
US4849337A (en) * | 1983-01-31 | 1989-07-18 | Minnesota Mining And Manufacturing Company | Assaying allergen specific IgE levels with fluorogenic enzyme labeled antibody |
US4558013A (en) * | 1983-04-08 | 1985-12-10 | Mast Immunosystems, Ltd. | Calibrated reaction measurement system |
JPS59208462A (ja) * | 1983-04-29 | 1984-11-26 | バイオウィッテッカー・インコーポレーテッド | キモパパインの分析法 |
US4649039A (en) * | 1984-07-03 | 1987-03-10 | E. I. Du Pont De Nemours And Company | Radiolabeling of methionine-containing proteins and peptides |
DE3800048A1 (de) * | 1987-05-14 | 1988-11-24 | Boehringer Mannheim Gmbh | Verfahren zur bestimmung eines antikoerpers in menschlichen koerperfluessigkeiten |
US5231344A (en) * | 1990-01-17 | 1993-07-27 | Hitachi Ltd. | Control apparatus for electric generator |
ATE555387T1 (de) | 2005-10-12 | 2012-05-15 | Allergan Inc | Tests der molekularen oder subzellulären nähe unter verwendung von depolarisierung nach resonanzenergietransfer (daret) |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3720760A (en) * | 1968-09-06 | 1973-03-13 | Pharmacia Ab | Method for determining the presence of reagin-immunoglobulins(reagin-ig)directed against certain allergens,in aqueous samples |
US3941876A (en) * | 1973-04-25 | 1976-03-02 | Gte New Ventures Corporation | In vitro method for determining allergic hypersensitivity |
-
1978
- 1978-01-26 US US05/872,668 patent/US4211762A/en not_active Expired - Lifetime
- 1978-12-14 CA CA317,964A patent/CA1114290A/en not_active Expired
-
1979
- 1979-01-08 DE DE2900546A patent/DE2900546C3/de not_active Expired
- 1979-01-15 SE SE7900343A patent/SE7900343L/xx not_active Application Discontinuation
- 1979-01-15 GB GB7901472A patent/GB2013337B/en not_active Expired
- 1979-01-24 FI FI790228A patent/FI790228A/fi not_active IP Right Cessation
- 1979-01-25 NO NO790252A patent/NO790252L/no unknown
- 1979-01-25 AU AU43668/79A patent/AU513556B2/en not_active Ceased
- 1979-01-25 DK DK32579A patent/DK32579A/da unknown
- 1979-01-25 FR FR7901965A patent/FR2445966A1/fr active Pending
Also Published As
Publication number | Publication date |
---|---|
DE2900546B2 (de) | 1980-09-11 |
CA1114290A (en) | 1981-12-15 |
AU4366879A (en) | 1979-08-02 |
GB2013337B (en) | 1982-11-17 |
SE7900343L (sv) | 1979-07-27 |
FR2445966A1 (fr) | 1980-08-01 |
US4211762A (en) | 1980-07-08 |
GB2013337A (en) | 1979-08-08 |
DK32579A (da) | 1979-07-27 |
DE2900546C3 (de) | 1981-05-21 |
AU513556B2 (en) | 1980-12-11 |
FI790228A (fi) | 1979-07-27 |
DE2900546A1 (de) | 1979-08-30 |
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