NO783976L - Fremgangsmaate ved fremstilling av et renset kapsulaert polysaccharid av streptococcus pneumoniae - Google Patents

Fremgangsmaate ved fremstilling av et renset kapsulaert polysaccharid av streptococcus pneumoniae

Info

Publication number: NO783976L
Authority: NO; Norway
Prior art keywords: polysaccharide; hours; added; stirring; isopropanol
Prior art date: 1977-11-28

Application number

NO783976A

Other languages

English (en)

Norwegian (no)

Inventor

Dennis J Carlo

Karl H Nollstadt

Thomas H Stoudt

Robert B Walton

Johanna Y Zeltner

Original Assignee

Merck & Co Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1977-11-28

Filing date

1978-11-27

Publication date

1979-05-29

1978-11-27 Application filed by Merck & Co Inc filed Critical Merck & Co Inc

1979-05-29 Publication of NO783976L publication Critical patent/NO783976L/no

Links

150000004676 glycans Chemical class 0.000 title claims description 241
229920001282 polysaccharide Polymers 0.000 title claims description 240
239000005017 polysaccharide Substances 0.000 title claims description 240
238000000034 method Methods 0.000 title claims description 14
241000193998 Streptococcus pneumoniae Species 0.000 title description 13
229940031000 streptococcus pneumoniae Drugs 0.000 title description 11
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 316
239000007788 liquid Substances 0.000 claims description 135
238000000855 fermentation Methods 0.000 claims description 52
230000004151 fermentation Effects 0.000 claims description 52
102000004142 Trypsin Human genes 0.000 claims description 22
108090000631 Trypsin Proteins 0.000 claims description 22
239000012588 trypsin Substances 0.000 claims description 22
241000194017 Streptococcus Species 0.000 claims description 18
LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 claims description 13
229960000800 cetrimonium bromide Drugs 0.000 claims description 10
150000007523 nucleic acids Chemical class 0.000 claims description 9
102000039446 nucleic acids Human genes 0.000 claims description 6
108020004707 nucleic acids Proteins 0.000 claims description 6
102000004169 proteins and genes Human genes 0.000 claims description 6
108090000623 proteins and genes Proteins 0.000 claims description 6
125000004432 carbon atom Chemical group C* 0.000 claims description 5
239000003093 cationic surfactant Substances 0.000 claims description 4
101710163270 Nuclease Proteins 0.000 claims description 3
239000012634 fragment Substances 0.000 claims description 2
239000012535 impurity Substances 0.000 claims 2
238000011026 diafiltration Methods 0.000 claims 1
238000004519 manufacturing process Methods 0.000 claims 1
KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 232
238000003756 stirring Methods 0.000 description 170
239000000203 mixture Substances 0.000 description 154
229960004592 isopropanol Drugs 0.000 description 116
HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 111
QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 109
239000002609 medium Substances 0.000 description 107
UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 86
239000006228 supernatant Substances 0.000 description 77
239000002244 precipitate Substances 0.000 description 73
239000000243 solution Substances 0.000 description 69
CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 63
ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 56
229960000583 acetic acid Drugs 0.000 description 54
239000012362 glacial acetic acid Substances 0.000 description 53
239000007787 solid Substances 0.000 description 51
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 49
229910001868 water Inorganic materials 0.000 description 49
229910000030 sodium bicarbonate Inorganic materials 0.000 description 44
235000017557 sodium bicarbonate Nutrition 0.000 description 43
VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 42
239000000843 powder Substances 0.000 description 42
239000001632 sodium acetate Substances 0.000 description 42
235000017281 sodium acetate Nutrition 0.000 description 42
239000008280 blood Substances 0.000 description 39
210000004369 blood Anatomy 0.000 description 39
238000005119 centrifugation Methods 0.000 description 34
235000013372 meat Nutrition 0.000 description 28
239000000284 extract Substances 0.000 description 27
235000015097 nutrients Nutrition 0.000 description 27
238000011534 incubation Methods 0.000 description 26
229960003742 phenol Drugs 0.000 description 23
238000012360 testing method Methods 0.000 description 23
239000000725 suspension Substances 0.000 description 22
238000001556 precipitation Methods 0.000 description 21
102000016911 Deoxyribonucleases Human genes 0.000 description 20
108010053770 Deoxyribonucleases Proteins 0.000 description 20
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 20
WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 20
102000006382 Ribonucleases Human genes 0.000 description 19
108010083644 Ribonucleases Proteins 0.000 description 19
238000003556 assay Methods 0.000 description 19
230000004520 agglutination Effects 0.000 description 18
229940075894 denatured ethanol Drugs 0.000 description 17
238000005352 clarification Methods 0.000 description 16
238000001914 filtration Methods 0.000 description 16
238000004090 dissolution Methods 0.000 description 15
239000000047 product Substances 0.000 description 15
241000894006 Bacteria Species 0.000 description 14
239000006161 blood agar Substances 0.000 description 14
238000006243 chemical reaction Methods 0.000 description 14
238000003306 harvesting Methods 0.000 description 14
230000001105 regulatory effect Effects 0.000 description 14
238000005406 washing Methods 0.000 description 14
239000008103 glucose Substances 0.000 description 13
238000010408 sweeping Methods 0.000 description 13
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 12
239000012528 membrane Substances 0.000 description 12
230000029087 digestion Effects 0.000 description 11
239000011521 glass Substances 0.000 description 11
238000001802 infusion Methods 0.000 description 11
239000012466 permeate Substances 0.000 description 11
TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 10
241000283973 Oryctolagus cuniculus Species 0.000 description 10
238000000502 dialysis Methods 0.000 description 10
239000008188 pellet Substances 0.000 description 10
230000035899 viability Effects 0.000 description 10
SHZGCJCMOBCMKK-UHFFFAOYSA-N D-mannomethylose Natural products CC1OC(O)C(O)C(O)C1O SHZGCJCMOBCMKK-UHFFFAOYSA-N 0.000 description 9
239000002054 inoculum Substances 0.000 description 9
239000012465 retentate Substances 0.000 description 9
238000000108 ultra-filtration Methods 0.000 description 9
WQZGKKKJIJFFOK-SVZMEOIVSA-N (+)-Galactose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-SVZMEOIVSA-N 0.000 description 8
239000011928 denatured alcohol Substances 0.000 description 8
239000008363 phosphate buffer Substances 0.000 description 8
239000013587 production medium Substances 0.000 description 8
239000007691 rabbit blood agar Substances 0.000 description 8
238000013019 agitation Methods 0.000 description 7
150000001720 carbohydrates Chemical class 0.000 description 7
229960003666 liquefied phenol Drugs 0.000 description 7
239000000463 material Substances 0.000 description 7
239000011780 sodium chloride Substances 0.000 description 7
239000002904 solvent Substances 0.000 description 7
PNNNRSAQSRJVSB-UHFFFAOYSA-N L-rhamnose Natural products CC(O)C(O)C(O)C(O)C=O PNNNRSAQSRJVSB-UHFFFAOYSA-N 0.000 description 6
MSWZFWKMSRAUBD-UHFFFAOYSA-N beta-D-galactosamine Natural products NC1C(O)OC(CO)C(O)C1O MSWZFWKMSRAUBD-UHFFFAOYSA-N 0.000 description 6
229920001817 Agar Polymers 0.000 description 5
239000008272 agar Substances 0.000 description 5
PNNNRSAQSRJVSB-BXKVDMCESA-N aldehydo-L-rhamnose Chemical compound C[C@H](O)[C@H](O)[C@@H](O)[C@@H](O)C=O PNNNRSAQSRJVSB-BXKVDMCESA-N 0.000 description 5
BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 5
229910052921 ammonium sulfate Inorganic materials 0.000 description 5
235000011130 ammonium sulphate Nutrition 0.000 description 5
238000011835 investigation Methods 0.000 description 5
239000011344 liquid material Substances 0.000 description 5
229910001629 magnesium chloride Inorganic materials 0.000 description 5
WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 4
230000001476 alcoholic effect Effects 0.000 description 4
OSGAYBCDTDRGGQ-UHFFFAOYSA-L calcium sulfate Chemical compound [Ca+2].[O-]S([O-])(=O)=O OSGAYBCDTDRGGQ-UHFFFAOYSA-L 0.000 description 4
239000002198 insoluble material Substances 0.000 description 4
239000002953 phosphate buffered saline Substances 0.000 description 4
MSWZFWKMSRAUBD-GASJEMHNSA-N 2-amino-2-deoxy-D-galactopyranose Chemical compound N[C@H]1C(O)O[C@H](CO)[C@H](O)[C@@H]1O MSWZFWKMSRAUBD-GASJEMHNSA-N 0.000 description 3
MSWZFWKMSRAUBD-IVMDWMLBSA-N 2-amino-2-deoxy-D-glucopyranose Chemical compound N[C@H]1C(O)O[C@H](CO)[C@@H](O)[C@@H]1O MSWZFWKMSRAUBD-IVMDWMLBSA-N 0.000 description 3
PNNNRSAQSRJVSB-SLPGGIOYSA-N Fucose Natural products C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)C=O PNNNRSAQSRJVSB-SLPGGIOYSA-N 0.000 description 3
SHZGCJCMOBCMKK-DHVFOXMCSA-N L-fucopyranose Chemical compound C[C@@H]1OC(O)[C@@H](O)[C@H](O)[C@@H]1O SHZGCJCMOBCMKK-DHVFOXMCSA-N 0.000 description 3
KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 3
238000010790 dilution Methods 0.000 description 3
239000012895 dilution Substances 0.000 description 3
239000003792 electrolyte Substances 0.000 description 3
238000005194 fractionation Methods 0.000 description 3
229930182830 galactose Natural products 0.000 description 3
229960002442 glucosamine Drugs 0.000 description 3
229940031348 multivalent vaccine Drugs 0.000 description 3
239000002245 particle Substances 0.000 description 3
230000028327 secretion Effects 0.000 description 3
102000004190 Enzymes Human genes 0.000 description 2
108090000790 Enzymes Proteins 0.000 description 2
SHZGCJCMOBCMKK-JFNONXLTSA-N L-rhamnopyranose Chemical compound C[C@@H]1OC(O)[C@H](O)[C@H](O)[C@H]1O SHZGCJCMOBCMKK-JFNONXLTSA-N 0.000 description 2
BELBBZDIHDAJOR-UHFFFAOYSA-N Phenolsulfonephthalein Chemical compound C1=CC(O)=CC=C1C1(C=2C=CC(O)=CC=2)C2=CC=CC=C2S(=O)(=O)O1 BELBBZDIHDAJOR-UHFFFAOYSA-N 0.000 description 2
201000005010 Streptococcus pneumonia Diseases 0.000 description 2
239000002253 acid Substances 0.000 description 2
WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 description 2
239000000427 antigen Substances 0.000 description 2
102000036639 antigens Human genes 0.000 description 2
108091007433 antigens Proteins 0.000 description 2
239000007853 buffer solution Substances 0.000 description 2
235000011132 calcium sulphate Nutrition 0.000 description 2
239000000356 contaminant Substances 0.000 description 2
239000012467 final product Substances 0.000 description 2
239000012530 fluid Substances 0.000 description 2
239000004615 ingredient Substances 0.000 description 2
238000011081 inoculation Methods 0.000 description 2
150000002500 ions Chemical class 0.000 description 2
229960003531 phenolsulfonphthalein Drugs 0.000 description 2
102000004196 processed proteins & peptides Human genes 0.000 description 2
108090000765 processed proteins & peptides Proteins 0.000 description 2
238000011160 research Methods 0.000 description 2
238000000926 separation method Methods 0.000 description 2
239000007974 sodium acetate buffer Substances 0.000 description 2
229960005486 vaccine Drugs 0.000 description 2
101710186708 Agglutinin Proteins 0.000 description 1
229910018626 Al(OH) Inorganic materials 0.000 description 1
KHOITXIGCFIULA-UHFFFAOYSA-N Alophen Chemical compound C1=CC(OC(=O)C)=CC=C1C(C=1N=CC=CC=1)C1=CC=C(OC(C)=O)C=C1 KHOITXIGCFIULA-UHFFFAOYSA-N 0.000 description 1
201000001178 Bacterial Pneumonia Diseases 0.000 description 1
BVKZGUZCCUSVTD-UHFFFAOYSA-M Bicarbonate Chemical compound OC([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-M 0.000 description 1
241000282465 Canis Species 0.000 description 1
AEMOLEFTQBMNLQ-YMDCURPLSA-N D-galactopyranuronic acid Chemical compound OC1O[C@H](C(O)=O)[C@H](O)[C@H](O)[C@H]1O AEMOLEFTQBMNLQ-YMDCURPLSA-N 0.000 description 1
IAJILQKETJEXLJ-UHFFFAOYSA-N Galacturonsaeure Natural products O=CC(O)C(O)C(O)C(O)C(O)=O IAJILQKETJEXLJ-UHFFFAOYSA-N 0.000 description 1
238000003794 Gram staining Methods 0.000 description 1
101710146024 Horcolin Proteins 0.000 description 1
-1 KHCO^ ^ 2C0^ Chemical compound 0.000 description 1
101710189395 Lectin Proteins 0.000 description 1
241001233242 Lontra Species 0.000 description 1
101710179758 Mannose-specific lectin Proteins 0.000 description 1
101710150763 Mannose-specific lectin 1 Proteins 0.000 description 1
101710150745 Mannose-specific lectin 2 Proteins 0.000 description 1
FLEAADSSUQORCN-WIYIBBIWSA-N N-[(2S,3R,4S,5R)-3,4,5,6-tetrahydroxy-1-oxohexan-2-yl]acetamide N-[(3S,4R,5S,6R)-2,4,5-trihydroxy-6-(hydroxymethyl)oxan-3-yl]acetamide Chemical compound CC(=O)N[C@H](C=O)[C@@H](O)[C@H](O)[C@H](O)CO.CC(=O)N[C@@H]1C(O)O[C@H](CO)[C@@H](O)[C@@H]1O FLEAADSSUQORCN-WIYIBBIWSA-N 0.000 description 1
229910019142 PO4 Inorganic materials 0.000 description 1
239000000910 agglutinin Substances 0.000 description 1
MBLBDJOUHNCFQT-WCTZXXKLSA-N aldehydo-N-acetyl-D-mannosamine Chemical compound CC(=O)N[C@H](C=O)[C@@H](O)[C@H](O)[C@H](O)CO MBLBDJOUHNCFQT-WCTZXXKLSA-N 0.000 description 1
239000003708 ampul Substances 0.000 description 1
239000007864 aqueous solution Substances 0.000 description 1
239000003125 aqueous solvent Substances 0.000 description 1
230000001580 bacterial effect Effects 0.000 description 1
230000015572 biosynthetic process Effects 0.000 description 1
AXCZMVOFGPJBDE-UHFFFAOYSA-L calcium dihydroxide Chemical compound [OH-].[OH-].[Ca+2] AXCZMVOFGPJBDE-UHFFFAOYSA-L 0.000 description 1
239000000920 calcium hydroxide Substances 0.000 description 1
235000011116 calcium hydroxide Nutrition 0.000 description 1
229910001861 calcium hydroxide Inorganic materials 0.000 description 1
239000001175 calcium sulphate Substances 0.000 description 1
229940041514 candida albicans extract Drugs 0.000 description 1
239000003153 chemical reaction reagent Substances 0.000 description 1
239000008121 dextrose Substances 0.000 description 1
LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 1
239000012153 distilled water Substances 0.000 description 1
238000000605 extraction Methods 0.000 description 1
239000000706 filtrate Substances 0.000 description 1
238000005189 flocculation Methods 0.000 description 1
230000016615 flocculation Effects 0.000 description 1
238000004817 gas chromatography Methods 0.000 description 1
239000012869 germination medium Substances 0.000 description 1
239000001963 growth medium Substances 0.000 description 1
VTHJTEIRLNZDEV-UHFFFAOYSA-L magnesium dihydroxide Chemical compound [OH-].[OH-].[Mg+2] VTHJTEIRLNZDEV-UHFFFAOYSA-L 0.000 description 1
239000000347 magnesium hydroxide Substances 0.000 description 1
235000012254 magnesium hydroxide Nutrition 0.000 description 1
229910001862 magnesium hydroxide Inorganic materials 0.000 description 1
230000003287 optical effect Effects 0.000 description 1
NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
239000010452 phosphate Substances 0.000 description 1
150000004804 polysaccharides Polymers 0.000 description 1
235000011118 potassium hydroxide Nutrition 0.000 description 1
230000001376 precipitating effect Effects 0.000 description 1
230000002028 premature Effects 0.000 description 1
238000002360 preparation method Methods 0.000 description 1
230000000717 retained effect Effects 0.000 description 1
229910000029 sodium carbonate Inorganic materials 0.000 description 1
230000001954 sterilising effect Effects 0.000 description 1
238000004659 sterilization and disinfection Methods 0.000 description 1
230000001502 supplementing effect Effects 0.000 description 1
239000008215 water for injection Substances 0.000 description 1
239000012138 yeast extract Substances 0.000 description 1

Classifications

- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/02—Bacterial antigens
- A61K39/09—Lactobacillales, e.g. aerococcus, enterococcus, lactobacillus, lactococcus, streptococcus
- A61K39/092—Streptococcus

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Epidemiology (AREA)
Animal Behavior & Ethology (AREA)
Medicinal Chemistry (AREA)
Microbiology (AREA)
Mycology (AREA)
Pharmacology & Pharmacy (AREA)
Chemical & Material Sciences (AREA)
Immunology (AREA)
General Health & Medical Sciences (AREA)
Public Health (AREA)
Veterinary Medicine (AREA)
Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Polysaccharides And Polysaccharide Derivatives (AREA)
Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)

NO783976A 1977-11-28 1978-11-27 Fremgangsmaate ved fremstilling av et renset kapsulaert polysaccharid av streptococcus pneumoniae NO783976L (no)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
US85549177A	1977-11-28	1977-11-28

Publications (1)

Publication Number	Publication Date
NO783976L true NO783976L (no)	1979-05-29

Family

ID=25321387

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
NO783976A NO783976L (no)	1977-11-28	1978-11-27	Fremgangsmaate ved fremstilling av et renset kapsulaert polysaccharid av streptococcus pneumoniae

Country Status (13)

Country	Link
EP (1)	EP0002404A1 (da)
JP (1)	JPS5495724A (da)
AU (1)	AU529128B2 (da)
CA (1)	CA1115210A (da)
DK (1)	DK527078A (da)
ES (1)	ES475509A1 (da)
FI (1)	FI783620A (da)
IT (1)	IT1109450B (da)
NO (1)	NO783976L (da)
NZ (1)	NZ188966A (da)
PT (1)	PT68831A (da)
YU (1)	YU283378A (da)
ZA (1)	ZA786664B (da)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4242501A (en) *	1979-08-08	1980-12-30	American Cyanamid Company	Purification of pneumococcal capsular polysaccharides
US4287173A (en) *	1979-09-24	1981-09-01	Merck & Co., Inc.	Vaccine for dental caries
FR2510606A1 (fr) *	1981-07-30	1983-02-04	Berri Balzac	Procede d'obtention de polyosides capsulaires, polyosides capsulaires ainsi obtenus et leur application a la preparation de vaccins
BE889979A (fr) *	1981-08-14	1982-02-15	Smith Kline Rit	Procede de preparation de polysaccharides bacteriens capsulaires antigeniques purifies, produits obtenus et leur utilisation
NL8202527A (nl) *	1982-06-22	1984-01-16	Univ Utrecht	Vaccins tegen door bacterien met kapsel-polysacchariden verwekte ziekten en werkwijze voor het bereiden daarvan.
US4686102A (en) *	1984-04-12	1987-08-11	American Cyanamid Company	Multivalent pneumococcal vaccine and preparation thereof
NZ214503A (en) *	1984-12-20	1990-02-26	Merck & Co Inc	Covalently-modified neutral bacterial polysaccharides, stable covalent conjugates of such polysaccharides and immunogenic proteins, and methods of preparing such polysaccharides and conjugates
US5714354A (en) *	1995-06-06	1998-02-03	American Home Products Corporation	Alcohol-free pneumococcal polysaccharide purification process
US6224880B1 (en)	1997-09-24	2001-05-01	Merck & Co., Inc.	Immunization against Streptococcus pneumoniae using conjugated and unconjugated pneumoccocal polysaccharide vaccines
BRPI0607026B1 (pt)	2005-04-08	2022-02-15	Wyeth	Processos para redução do teor de proteína e preservação do teor de polissacarídeo capsular em um caldo de lisado celular de streptococcus pneumoniae complexo antes da purificação
EP2425854A1 (en)	2005-04-08	2012-03-07	Wyeth LLC	Multivalent pneumococcal polysaccharide-protein conjugate composition
CN101663327B (zh) *	2007-03-23	2014-05-07	惠氏公司	产生肺炎链球菌(streptococcus pneumoniae)荚膜多糖的缩短的纯化方法
KR101593770B1 (ko)	2008-12-18	2016-02-15	와이어쓰 엘엘씨	스트렙토코커스 뉴모니에 혈청형 19ａ 폴리사카라이드 분자량을 조절하는 방법
KR101307703B1 (ko)	2008-12-18	2013-09-11	와이어쓰 엘엘씨	탄소를 사용하여 스트렙토코커스 뉴모니에 폴리사카라이드 분자량을 조절하는 방법
WO2012150861A1 (en) *	2011-05-02	2012-11-08	Stichting Top Institute Food And Nutrition	Probiotics with enhanced survival properties
EP3140429B1 (en)	2014-05-05	2020-02-19	Medtronic Inc.	Methods for scd, crt, crt-d, or sca therapy identification and/or selection
CN114853917A (zh) *	2022-04-22	2022-08-05	兰州生物制品研究所有限责任公司	制备肺炎球菌荚膜多糖和肺炎球菌疫苗的方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
FR2388563A1 (fr) *	1977-04-29	1978-11-24	Fabre Sa Pierre	Vaccins acellulaires perfectionnes contenant des polysaccharides capsulaires

1978
- 1978-10-24 CA CA314,028A patent/CA1115210A/en not_active Expired
- 1978-11-16 EP EP78400185A patent/EP0002404A1/en not_active Withdrawn
- 1978-11-20 AU AU41728/78A patent/AU529128B2/en not_active Expired
- 1978-11-21 NZ NZ188966A patent/NZ188966A/xx unknown
- 1978-11-27 DK DK527078A patent/DK527078A/da not_active Application Discontinuation
- 1978-11-27 ZA ZA786664A patent/ZA786664B/xx unknown
- 1978-11-27 IT IT52075/78A patent/IT1109450B/it active
- 1978-11-27 PT PT68831A patent/PT68831A/pt unknown
- 1978-11-27 FI FI783620A patent/FI783620A/fi unknown
- 1978-11-27 NO NO783976A patent/NO783976L/no unknown
- 1978-11-28 ES ES475509A patent/ES475509A1/es not_active Expired
- 1978-11-28 JP JP14614478A patent/JPS5495724A/ja active Pending
- 1978-12-04 YU YU02833/78A patent/YU283378A/xx unknown

Also Published As

Publication number	Publication date
DK527078A (da)	1979-07-04
CA1115210A (en)	1981-12-29
PT68831A (en)	1978-12-01
JPS5495724A (en)	1979-07-28
AU4172878A (en)	1979-06-07
ES475509A1 (es)	1980-07-16
NZ188966A (en)	1982-03-23
IT1109450B (it)	1985-12-16
YU283378A (en)	1983-04-30
IT7852075A0 (it)	1978-11-27
ZA786664B (en)	1980-07-30
AU529128B2 (en)	1983-05-26
FI783620A (fi)	1979-05-29
EP0002404A1 (en)	1979-06-13

Publication	Publication Date	Title
NO783976L (no)	1979-05-29	Fremgangsmaate ved fremstilling av et renset kapsulaert polysaccharid av streptococcus pneumoniae
DK173681B1 (da)	2001-06-11	Fremgangsmåde til fremstilling af hyaluronsyre ud fra en hyaluronsyreproducerende streptococbakterie
Wilson et al.	1985	Bone resorbing activity of purified capsular material from Actinobacillus actinomycetemcomitans
Roberton et al.	1982	In vitro utilization of mucin by Bacteroides fragilis
Dudman et al.	1956	The composition of the extracellular polysaccharides of Aerobacter–Klebsiella strains
EP0024493B1 (en)	1985-06-12	Multivalent pneumococcal vaccine
Henry et al.	1946	Histochemistry of the Gram-staining reaction for micro-organisms
JPS60251898A (ja)	1985-12-12	醗酵法によるヒアルロン酸の製造方法
JPH09512708A (ja)	1997-12-22	グルカンの酵素処理
JP2012512659A (ja)	2012-06-07	二酸化炭素を用いて肺炎連鎖球菌（Ｓｔｒｅｐｔｏｃｏｃｃｕｓｐｎｅｕｍｏｎｉａｅ）多糖の分子量を制御するための方法
EP0041897B1 (en)	1986-12-03	Polysaccharide antigen from streptococcus and vaccines
EP0038265B1 (en)	1985-10-02	Group b streptococcal capsular polysaccharides
US4123520A (en)	1978-10-31	Method for preparing high molecular weight meningococcal Group C vaccine
EP0407037B1 (en)	1994-09-21	Process for removing bacterial endotoxin from gram-negative polysaccharides
EP0008969A2 (en)	1980-03-19	Vaccine and method of making
Heidelberger et al.	1950	Improved methods for the preparation of the specific polysaccharides of pneumococcus
US4678748A (en)	1987-07-07	Process for the production of immunobiological preparations applicable in the diagnosis, prevention and/or treatment of Candida guilliermondii infections
DISCHE et al.	1955	Polysaccharides of the Vitreous Tibers
US4235994A (en)	1980-11-25	High molecular weight meningococcal group C vaccine and method for preparation thereof
US5019502A (en)	1991-05-28	Process for removing bacterial endotoxin from gram-negative polysaccharides
US5039610A (en)	1991-08-13	Process for removing bacterial endotoxin from gram-negative polysaccharides
NO142961B (no)	1980-08-11	Steroidbindende globulin til anvendelse ved in vitro-diagnostikk og ved fremstilling av antisera
US2826571A (en)	1958-03-11	Protein product and process
Young et al.	1982	Agglutination of mycelial cell wall fragments and spores of Colletotrichum lindemuthianum by plant extracts, and by various proteins
SU1750689A1 (ru)	1992-07-30	Способ получени полисахаридно-белковой вакцины против NeISSeRIa меNINGIтIDIS серогруппы В