NL2033491A - Device and method for remediation of heavy metals in cultivated land soil by strengthening sedum plumbizincicola with biochar combined with microorganism - Google Patents

Device and method for remediation of heavy metals in cultivated land soil by strengthening sedum plumbizincicola with biochar combined with microorganism Download PDF

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NL2033491A
NL2033491A NL2033491A NL2033491A NL2033491A NL 2033491 A NL2033491 A NL 2033491A NL 2033491 A NL2033491 A NL 2033491A NL 2033491 A NL2033491 A NL 2033491A NL 2033491 A NL2033491 A NL 2033491A
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soil
crushing
sedum plumbizincicola
remediation
charcoal
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NL2033491A
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NL2033491B1 (en
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Liu Yingying
Zhang Jiachun
Luo Wenmin
Zhang Zhenming
Mu Guiting
Xu Daigang
Wu Xianliang
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Univ Guizhou
Guizhou Inst Biology
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
    • B09C—RECLAMATION OF CONTAMINATED SOIL
    • B09C1/00—Reclamation of contaminated soil
    • B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
    • B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
    • B09C—RECLAMATION OF CONTAMINATED SOIL
    • B09C1/00—Reclamation of contaminated soil
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
    • B09C—RECLAMATION OF CONTAMINATED SOIL
    • B09C1/00—Reclamation of contaminated soil
    • B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K17/00—Soil-conditioning materials or soil-stabilising materials
    • C09K17/40—Soil-conditioning materials or soil-stabilising materials containing mixtures of inorganic and organic compounds
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K17/00—Soil-conditioning materials or soil-stabilising materials
    • C09K17/40—Soil-conditioning materials or soil-stabilising materials containing mixtures of inorganic and organic compounds
    • C09K17/42—Inorganic compounds mixed with organic active ingredients, e.g. accelerators
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
    • A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K2101/00—Agricultural use

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Soil Sciences (AREA)
  • Mycology (AREA)
  • Environmental & Geological Engineering (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Inorganic Chemistry (AREA)
  • Botany (AREA)
  • General Life Sciences & Earth Sciences (AREA)
  • Materials Engineering (AREA)
  • Organic Chemistry (AREA)
  • Processing Of Solid Wastes (AREA)
  • Cultivation Of Plants (AREA)

Abstract

Disclosed is a device and a method for remediation of heavy metals in cultivated land soil by 5 strengthening Sedum plumbizincicola with biochar combined with microorganism, and relates to the technical field of soil remediation devices and methods, to solve the problem that there are no devices and methods for soil remediation in the market at present. Step 1: collecting calcareous soil and yellow soil; step 2: selecting lettuce and water spinach, and selecting Thiobaci/Ius ferrooxidans; step 3: removing stones and animal and plant residues, and then drying 10 them in the shade respectively; crushing and sieving the soil by the soil crushing mechanism; step 4: filling each pot with soil and biomass charcoal to be tested, and mixing evenly for later use; step 5: cultivating three vegetable seedlings in each pot, applying corresponding proportions of bacterial solutions and three Sedum plumbizincicola to the pot, cultivating vegetable seedlings and Sedum plumbizincicola by the cultivation mechanism, and harvesting the vegetables after 15 they grow for about 80 days; and step 6: harvesting vegetables and Sedum plumbizincicola at the same time, respectively crushing vegetables and Sedum plumbizincicola dry samples for testing; drying rhizosphere soil in the shade, sieving and testing.

Description

DEVICE AND METHOD FOR REMEDIATION OF HEAVY METALS IN CULTIVATED
LAND SOIL BY STRENGTHENING SEDUM PLUMBIZINCICOLA WITH BIOCHAR
COMBINED WITH MICROORGANISM
TECHNICAL FIELD
The invention relates to the technical field of soil remediation devices and methods, and in particular to a device and a method for remediation of heavy metals in cultivated land soil by strengthening Sedum plumbizincicola with biochar combined with microorganism.
BACKGROUND
Soil remediation is a technical measure to restore the polluted soil to normal functions. In the soil remediation industry, there are more than one hundred kinds of soil remediation technologies, and there are more than ten kinds of commonly used technologies; the technologies are roughly divided into three methods: physical, chemical and biological. Since 1980s, many countries in the world, especially developed countries, have formulated and carried out plans for the treatment and remediation of contaminated soil, thus forming a new soil remediation industry. At present, there are many remediation methods of heavy metals in soil theoretically, but they are all quite expensive, cause great damage to soil vegetation, soil structure and soil microbial environment, and also easily lead to “secondary pollution”. biochar combined with microorganism is a green and environment-friendly remediation method for heavy metals in cultivated soil, and has been widely recognized in recent years. biochar is a kind of highly aromatic and refractory solid material produced by pyrolysis and carbonization of biomass under complete or partial anoxic conditions. Due to its large specific surface area and surface functional groups, biochar fixes heavy metals, and has strong adsorption capacity; it adsorbs available heavy metals in the surrounding soil. At the same time, the surface space of biochar provides a habitat for microorganisms, and this is conducive to the growth of soil microorganisms. So far, it is considered as an environmental-friendly and promising green treatment technology to use microorganisms and biochar to jointly control soil heavy metals.
At present, plant intercropping remediation technology has become a hot research topic to solve the problem that single planting of hyperaccumulator affects remediation effect. Sedum plumbizincicola, a new species of Crassulaceae family plant, is also a hyperaccumulator of heavy metals found in mining areas of China. For example, using Apium graceolens and Sedum plumbizincicola intercropping to repair Cd-polluted soil, the Cd content in Apium graceolens meets the food hygiene standard, and the Cd concentration in Sedum plumbizincicola intercropping is 37.4 times that of monoculture. It is an economical and effective way to remediate heavy metals in cultivated land soil by strengthening Sedum plumbizincicola with biochar and microorganism, and it not only improves remediation effect, but also meets the goal of safe production of crops. The technology has low cost, little negative impact on soil fertilizer and metabolic activity, and avoids the impact of heavy metal pollutants migrating to the environment and human body. Therefore, from the perspective of pollution remediation and the quality and safety of agricultural products, it is of great significance to remediate heavy metals in cultivated soil by strengthening Sedum plumbizincicola with biochar and microorganism, and solve the problem of remediation and improvement of contaminated soil. At present, there is no remediation device and method aiming at this remediation technology in the market, so the device and method for remediation of heavy metals in cultivated land soil by strengthening
Sedum plumbizincicola with biochar combined with microorganism are urgently needed in the market to solve these problems.
SUMMARY
The objective of the present invention is to provide a device and a method for remediation of heavy metals in cultivated land soil by strengthening Sedum plumbizincicola with biochar combined with microorganism, so as to solve the problems in the background that there are no devices or methods for soil remediation in the market at present.
In order to achieve the above objective, the technical scheme provided by the invention is as follows: a device for remediation of heavy metals in cultivated land soil by strengthening
Sedum plumbizincicola with biochar combined with microorganism includes a cultivation mechanism and a soil crushing mechanism, wherein a lower end of the cultivation mechanism is provided with a support seat, and an upper part of the support seat is provided with cultivation frames at equal intervals; four corners of each cultivation frame and four corners of the upper end of each support seat are provided with fixing rod connecting sleeves, and fixing rod slots are arranged in the fixing rod connecting sleeves; fixing rods are arranged between the cultivation frame, the support seat and adjacent cultivation frames, two light-filling lamps are symmetrically arranged on both sides of a lower end of each cultivation frame, and water nozzles are arranged between adjacent light-filling lamps; a brightness sensor is arranged at one side of the water nozzle in the middle position, and the brightness sensor is electrically connected with the light-filling lamps; five cultivation pots are arranged in the upper end of the cultivation frames at equal intervals, and the cultivation pots are correspondingly arranged with the water nozzles; soil moisture sensors are arranged in the cultivation pots, one side inside the soil crushing mechanism is fixedly provided with a fixed crushing plate, the other side inside the soil crushing mechanism is slidably provided with a sliding crushing plate, opposite sides of the soil crushing mechanism and the fixed crushing plate are both provided with crushing clamping plates, and the two crushing clamping plates are meshed; a sieve tray is obliquely arranged below the fixed crushing plate of the soil crushing mechanism.
Preferably, upper and lower ends of each fixing rod are fixedly connected with the fixing rod slots through clamping grooves, a front end of one side of each cultivation frame is provided with a first magnetic block, and a rear end of the other side of the cultivation frame is provided with a second magnetic block.
Preferably, a front end of the other side of the cultivation frame is provided with a first magnetic block groove, and the first magnetic block groove is correspondingly arranged with the first magnetic block; a rear end of one side of the cultivation frame is provided with a second magnetic block groove, and the second magnetic block groove is correspondingly arranged with the second magnetic block.
Preferably, a water tank is arranged below the cultivation pots, and the water tank is communicated with the water nozzles.
Preferably, the sieve tray is fixedly connected with an inner wall of the soil crushing mechanism, a discharging conveyor belt and a material receiving basin are arranged below the sieve tray, and the discharging conveyor belt is located at one side of the material receiving basin; a filter plate is arranged inside the sieve tray; and a filter strip groove is arranged inside one side of the filter plate; the filter strip groove is positioned above the discharging conveyor belt, and another end of the filter plate is located above the material receiving basin; an outer end of the sieve tray is provided with a sieve tray frame; and one end of the sieve tray close to the material receiving basin inclines downwards.
Preferably, one side of the sliding crushing plate is provided with a crushing motor, an output end of the crushing motor is provided with a driving disc, a transmission rod is arranged between the driving disc and the sliding crushing plate, and two ends of the transmission rod are respectively connected with the driving disc and the sliding crushing plate in a rotary way.
Preferably, upper and lower ends of one side of the sliding crushing plate are provided with sliding blocks, two sides of the soil crushing mechanism are symmetrically provided with two sliding chutes, the sliding chutes are connected with the sliding blocks in a sliding way, and upper ends of the fixed crushing plate and the sliding crushing plate are provided with material guide plates.
A method for remediation of heavy metals in cultivated land soil by strengthening Sedum plumbizincicola with biochar combined with microorganism, including the following steps: step 1: collecting calcareous soil and yellow soil widely distributed in cultivated land as test soil; step 2: selecting lettuce of leafy vegetables and water spinach of rhizomes for the experiment, selecting Thiobacillus ferrooxidans with strong tolerance to heavy metals as the test microorganism, selecting peanut hull straw as biomass charcoal raw material at carbonization temperature of 350 - 500°C, and passing through a 2 mm sieve; step 3: removing stones and animal and plant residues from the yellow soil and calcareous soil to be tested, and then drying them in the shade respectively; crushing and sieving the soil by the soil crushing mechanism; the crushing motor driving the driving disc to rotate, and a lug at the front end of the driving disc being rotationally connected with the transmission rod; when the lug rotates in a circle, the transmission rod driving the sliding crushing plate to slide inside the soil crushing mechanism, and crushing the soil by two meshing crushing clamping plates; the crushed soil falling on the discharging conveyor belt through the filter strip groove, and the unfiltered large size soil particles falling inside the material receiving basin; step 4: setting biochar at six levels, namely, 0%, 4%, 6%, 8%, 10% and 20% biomass charcoal, and they are referred to as charcoal zero, charcoal one, charcoal two, charcoal three, charcoal four and charcoal five for short; filling each pot with 4 kg of soil and biomass charcoal, and filling each pot with a corresponding amount of soil and biomass charcoal according to the experimental design, and mixing evenly for later use; step 5: placing the mixed soil in a cultivation pot of a cultivation mechanism, and cultivating three vegetable seedlings in each pot; setting Thiobacillus ferrooxidans at six levels, namely, O ml, 10 ml, 20 ml, 40 ml, 60 ml and 80 ml bacterial solutions, and they are referred to as bacteria zero, bacteria one, bacteria two, bacteria three, bacteria four and bacteria five for short; according to the experimental design, applying corresponding proportions of bacterial solutions and three Sedum plumbizincicola to the pot; cultivating vegetable seedlings and
Sedum plumbizincicola by the cultivation mechanism, planting the vegetable seedlings and
Sedum plumbizincicola in the soil of a cultivation pot, adjusting the brightness of the light-filling lamp by the brightness sensor according to the brightness of the light received by the plant to fill the light for the vegetable seedlings, monitoring the soil humidity by a soil humidity sensor, and adjusting water nozzles to spray water, and harvesting the vegetables after they grow for about 80 days; and step 6: harvesting vegetables and Sedum plumbizincicola at the same time, washing and drying at 80°C, respectively crushing vegetables and Sedum plumbizincicola dry samples for testing; drying rhizosphere soil in the shade, sieving and testing.
Compared with the prior art, the invention has the following advantages.
Firstly, the invention deals with the soil polluted by heavy metals by using several technologies of biomass charcoal, Thiobacillus ferrooxidans, Sedum plumbizincicola and combined treatment of three methods, studies the distribution characteristics, migration and transformation rules of heavy metals in the soil-vegetable system under different treatment methods, and evaluates the inhibition and control effect of biomass charcoal, microorganisms and repair plants on heavy metals in soil and their effects on biomass, nutrient elements and heavy metals Cr, As, Cd, Pb and Hg of vegetables. Finally, the invention selects an appropriate technical scheme for soil heavy metal treatment in karst areas. The charcoal-bacteria combined technology has the largest impact on vegetable biomass accumulation and total biomass, followed by soil type and vegetable type; the charcoal-bacteria combined technology has the greatest impact on total N and total P of vegetables, and biomass charcoal has the greatest impact on total K of vegetables; the charcoal-bacteria combined technology has the greatest impact on the heavy metals Cr, As, Cd, Pb, Hg in vegetables; in a word, the charcoal-bacteria combined technology has a significant impact on many indicators of vegetables, and the charcoal-bacteria combined technology is a more appropriate composite repair technology.
Secondly, in the invention, the cultivation mechanism automatically replenishes water and light to promote the growth of Sedum plumbizincicola and accelerate the repair the soil polluted 5 by heavy metals, thereby improving the repair effect of the soil; the cultivation frame, support seat and fixed rod are spliced together to facilitate the loading, unloading and carrying of the device. At the same time, the correspondence of the first magnetic block and the second magnetic block with the second magnetic block groove and the first magnetic block groove realizes the splicing of multiple cultivation institutions, the soil crushing mechanism shall first crush and screen the soil before remediation, so that the remediation microorganisms, biochar and Sedum plumbizincicola fully absorb heavy metals.
BRIEF DESCRIPTION OF THE FIGURES
Fig. 1 is a front view of the cultivation mechanism in the present invention.
Fig. 2 is a plan view of the cultivation frame in the present invention.
Fig. 3 is a bottom view of the cultivation frame in the present invention.
Fig. 4 is a cross-sectional view of Fig. 1 taken along A-A in the present invention.
Fig. 5 is a cross-sectional view of Fig. 2 taken along the B-B direction in the present invention.
Fig. 6 is a structural diagram of the soil crushing mechanism in the present invention.
Fig. 7 is a perspective view of the sieve tray of the present invention.
Fig. 8 is a side view of the sliding crushing plate in the present invention.
In the figures: 1. cultivation frame; 2. support seat; 3. fixing rod; 4. light-filling lamp; 5. cultivation pot; 6. fixing rod slot; 7. first magnetic block; 8. second magnetic block; 9. cultivation mechanism; 10. brightness sensor; 11. water nozzle; 12. water tank; 13. second magnetic block groove; 14. first magnetic block groove; 15. fixing rod connecting sleeve; 18. soil moisture sensor; 17. soil crushing mechanism; 18. fixed crushing plate; 19. sliding crushing plate; 20. crushing clamping plate; 21. driving disc; 22. crushing motor; 23. transmission rod; 24. sliding chute; 25. sieve tray; 26. discharging conveyor belt; 27. material receiving basin; 28. filter strip groove; 29. filter strip groove; 30. filter plate; 31. material guide plate; 32. sliding block.
DESCRIPTION OF THE INVENTION
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, but not all of them.
Referring to figures 1 - 8, the invention provides an embodiment: a device for remediation of heavy metals in cultivated land soil by strengthening Sedum plumbizincicola with biochar combined with microorganism includes a cultivation mechanism 9 and a soil crushing mechanism 17, wherein a lower end of the cultivation mechanism 9 is provided with a support seat 2, and an upper part of the support seat 2 is provided with cultivation frames 1 at equal intervals; four corners of each cultivation frame 1 and four corners of the upper end of each support seat 2 are provided with fixing rod connecting sleeves 15, and fixing rod slots 6 are arranged in the fixing rod connecting sleeves 15; fixing rods 3 are arranged between the cultivation frame 1, the support seat 2 and adjacent cultivation frames 1, two light-filling lamps 4 are symmetrically arranged on both sides of a lower end of each cultivation frame 1, and water nozzles 11 are arranged between adjacent light-filling lamps 4; a brightness sensor 10 is arranged at one side of the water nozzle 11 in the middle position, and the brightness sensor 10 is electrically connected with the light-filling lamps 4; five cultivation pots 5 are arranged in the upper end of the cultivation frames 1 at equal intervals, and the cultivation pots 5 are correspondingly arranged with the water nozzles 11; soil moisture sensors 16 are arranged in the cultivation pots 5, one side inside the soil crushing mechanism 17 is fixedly provided with a fixed crushing plate 18, the other side inside the soil crushing mechanism 17 is slidably provided with a sliding crushing plate 19, opposite sides of the soil crushing mechanism 17 and the fixed crushing plate 18 are both provided with crushing clamping plates 20, and the two crushing clamping plates 20 are meshed; a sieve tray 25 is obliquely arranged below the fixed crushing plate 18 of the soil crushing mechanism 17.
Further, upper and lower ends of each fixing rod 3 are fixedly connected with the fixing rod slots 6 through clamping grooves, a front end of one side of each cultivation frame 1 is provided with a first magnetic block 7, and a rear end of the other side of the cultivation frame 1 is provided with a second magnetic block 8.
Further, a front end of the other side of the cultivation frame 1 is provided with a first magnetic block groove 14, and the first magnetic block groove 14 is correspondingly arranged with the first magnetic block 7; a rear end of one side of the cultivation frame 1 is provided with a second magnetic block groove 13, and the second magnetic block groove 13 is correspondingly arranged with the second magnetic block 8.
Further, a water tank 12 is arranged below the cultivation pots 5, and the water tank 12 is communicated with the water nozzles 11.
Further, the sieve tray 25 is fixedly connected with an inner wall of the soil crushing mechanism 17, a discharging conveyor belt 26 and a material receiving basin 27 are arranged below the sieve tray 25, and the discharging conveyor belt 26 is located at one side of the material receiving basin 27; a filter plate 30 is arranged inside the sieve tray 25; and a filter strip groove 29 is arranged inside one side of the filter plate 30; the filter strip groove 29 is positioned above the discharging conveyor belt 26, and another end of the filter plate 30 is located above the material receiving basin 27; an outer end of the sieve tray 25 is provided with a sieve tray frame 28; and one end of the sieve tray 25 close to the material receiving basin 27 inclines downwards.
Further, one side of the sliding crushing plate 19 is provided with a crushing motor 22, an output end of the crushing motor 22 is provided with a driving disc 21, a transmission rod 23 is arranged between the driving disc 21 and the sliding crushing plate 19, and two ends of the transmission rod 23 are respectively connected with the driving disc 21 and the sliding crushing plate 19 in a rotary way.
Further, upper and lower ends of one side of the sliding crushing plate 19 are provided with sliding blocks 32, two sides of the soil crushing mechanism 17 are symmetrically provided with two sliding chutes 24, the sliding chutes 24 are connected with the sliding blocks 32 in a sliding way, and upper ends of the fixed crushing plate 18 and the sliding crushing plate 19 are provided with material guide plates 31.
A method of the device for remediation of heavy metals in cultivated land soil by strengthening Sedum plumbizincicola with biochar combined with microorganism includes the following steps: step 1: collecting calcareous soil and yellow soil widely distributed in cultivated land as test soil; physical and chemical properties of tested soil are shown in the following table:
Types Cr(mg/kg) As{(mg/kg) Cd(mg/kg) Pb(mg/kg) Hg(mg/kg) ‘calcareous soil 34.12 16.68 0.12 23.60 0.13
Yellow soil 64.12 56.68 0.32 52.60 0.33 step 2: selecting lettuce of leafy vegetables and water spinach of rhizomes for the experiment, selecting Thiobacillus ferrooxidans with strong tolerance to heavy metals as the test microorganism, selecting peanut hull straw as biomass charcoal raw material at carbonization temperature of 350 - 500°C, and passing through a 2 mm sieve; the strains provided by Shanghai Biological Network Microbial Culture Collection Center (RCCC) are described as follows:
Name of Date of Preservation Validity Incubation Incubation
Thiobacillus Storing at - physical and chemical properties of biomass charcoal are shown in the following table:
charcoal potassium step 3: removing stones and animal and plant residues from the yellow soil and calcareous soil to be tested, and then drying them in the shade respectively; crushing and sieving the soil by the soil crushing mechanism 17; the crushing motor 22 driving the driving disc 21 to rotate, and a lug at the front end of the driving disc 21 being rotationally connected with the transmission rod 23; when the lug rotates in a circle, the transmission rod 23 driving the sliding crushing plate 19 to slide inside the soil crushing mechanism 17, and crushing the soil by two meshing crushing clamping plates 20; the crushed soil falling on the discharging conveyor belt 26 through the filter strip groove 29, and the unfiltered large size soil particles falling inside the receiving basin 27; step 4: setting biochar at six levels, namely, 0%, 4%, 6%, 8%, 10% and 20% biomass charcoal, and they are referred to as charcoal zero, charcoal one, charcoal two, charcoal three, charcoal four and charcoal five for short; filling each pot with 4 kg of soil and biomass charcoal, and filling each pot with a corresponding amount of soil and biomass charcoal according to the experimental design, and mixing evenly for later use; step 5: placing the mixed soil in a cultivation pot 5 of a cultivation mechanism 9, and cultivating three vegetable seedlings in each pot; setting Thiobacillus ferrooxidans at six levels, namely, O ml, 10 ml, 20 ml, 40 ml, 60 ml and 80 ml bacterial solutions, and they are referred to as bacteria zero, bacteria one, bacteria two, bacteria three, bacteria four and bacteria five for short; according to the experimental design, applying corresponding proportions of bacterial solutions and three Sedum plumbizincicola to the pot; cultivating vegetable seedlings and
Sedum plumbizincicola by the cultivation mechanism 9, planting the vegetable seedlings and
Sedum plumbizincicola in the soil of a cultivation pot 5, adjusting the brightness of the light- filling lamp 4 by the brightness sensor 10 according to the brightness of the light received by the plant to fill the light for the vegetable seedlings, monitoring the soil humidity by a soil humidity sensor 16, and adjusting water nozzles 11 to spray water, and harvesting the vegetables after they grow for about 80 days;
The experimental orthogonal design of biomass charcoal- Thiobacillus ferrooxidans-Sedum plumbizincicola combined application to control heavy metals in soil is shown in the following table:
van tin
Appli- 9 / quan cation
Whether tity Plan-
Bio- Biomass amount to inter- of Vege- ting
Soil pot mass charcoal of
Treat Soil crop Sedu table amount loading char- pot Thioba- / ment type / / Sedum m varie- of vege- kg coal loading/ cillus == / / plumbizi plum ties tables/ ratio/% kg ferro- / ncicola ~~ bizin plant oxidans / cicol /ml a/pla nt
Calcare 1 / 3.76 6 0.24 10 Yes 3 Lettuce 3 ous soil
Yellow Water 2 3.68 8 0.32 10 Yes 3 3 soil spinach
Calcare 3 / 4 0 -- 0.5 No -- Lettuce 3 ous soil
Yellow Water 4 3.6 10 0.4 0.5 Yes 3 3 soil spinach
Yellow Water 3.2 20 0.8 20 Yes 3 3 soil spinach
Yellow Water 6 3.84 4 0.16 0 Yes 3 3 soil spinach
Yellow 7 / 3.2 20 0.8 0 Yes 3 Lettuce 3 soil
Calcare 8 / 3.68 8 0.32 c No -- Lettuce 3 ous sail
Yellow 9 / 3.84 4 0.16 1 No -- Lettuce 3 soil
Yellow / 4 0 -- c No -- Lettuce 3 sail
Yellow Water 11 3.76 6 0.24 0 No -- 3 soil spinach
Calcare Water 12 4 0 -- 1 Yes 3 3 ous soil spinach
Calcare 13 / 4 0 -- 5 Yes 3 Lettuce 3 ous soil
Calcare 14 / 3.84 4 0.16 0 Yes 3 Lettuce 3 ous soil
Calcare / 3.6 10 0.4 0 Yes 3 Lettuce 3 ous soil
Calcare Water 16 3.68 8 0.32 1 Yes 3 3 ous soil spinach
Yellow 17 / 3.76 6 0.24 1 No -- Lettuce 3 soil
Yellow Water 18 3.6 10 0.4 10 No -- 3 soil spinach
Yellow 19 / 4 0 -- 5 Yes 3 Lettuce 3 soil
Calcare Water 4 0 -- 20 Yes 3 3 ous soil spinach
Yellow Water 21 3.84 4 0.16 0.5 Yes 3 3 soil spinach
Calcare 22 / 3.68 8 0.32 20 No -- Lettuce 3 ous soil
Yellow 23 / 4 0 -- 0.5 Yes 3 Lettuce 3 soil
Yellow 24 / 3.78 6 0.24 0 Yes 3 Lettuce 3 soil
Calcare Water 3.2 20 0.8 0.5 No -- 3 ous soil spinach
Yellow 26 / 4 0 -- 20 No -- Lettuce 3 soil
Calcare Water 27 3.84 4 0.16 5 No -- 3 ous soil spinach
Yellow 28 / 3.68 8 0.32 5 Yes 3 Lettuce 3 soil
Calcare Water 29 4 0 -- 1 No -- 3 ous soil spinach
Yellow / 3.2 20 0.8 1 No -- Lettuce 3 soil
Yellow 31 / 3.84 4 0.16 20 No -- Lettuce 3 soil
Calcare Water 32 4 0 -- 0 No -- 3 ous soil spinach
Yellow 33 / 3.6 10 0.4 1 Yes 3 Lettuce 3 soil
Yellow 34 / 3.68 8 0.32 0.5 No -- Lettuce 3 soil
Calcare / 3.2 20 0.8 0 Yes 3 Lettuce 3 ous soil
Yellow Water 36 3.2 20 0.8 5 No -- 3 soil spinach
Yellow 37 / 3.6 10 0.4 20 No -- Lettuce 3 soil
Yellow Water 38 3.68 8 0.32 0 No -- 3 soil spinach
Yellow Water 39 3.76 6 24 5 No -- 3 soil spinach
Yellow / 4 0 -- 0 No -- Lettuce 3 soil
Yellow Water 41 4 0 == 0 No -- 3 soil spinach
Yellow Water 42 4 0 -- 10 No -- 3 soil spinach
Yellow 43 4 0 -- 10 Yes 3 Lettuce 3 soil
Calcare 44 3.76 6 0.24 0.5 No -- Lettuce 3 ous soil
Calcare 45 3.6 10 0.4 5 No -- Lettuce 3 ous soil
Calcare Water 46 3.6 10 0.4 0 No -- 3 ous soil spinach
Calcare 47 3.84 4 0.16 10 No -- Lettuce 3 ous soil
Calcare Water 48 3.76 6 0.24 20 Yes 3 3 ous soil spinach
Calcare 49 3.2 20 0.8 10 No -- Lettuce 3 ous soil step 6: harvesting vegetables and Sedum plumbizincicola at the same time, washing and drying at 60°C, respectively crushing vegetables and Sedum plumbizincicola dry samples for testing; drying rhizosphere soil in the shade, sieving and testing.
Experimental results of combined application of biomass charcoal- Thiobacillus ferrooxidans-Sedum plumbizincicola to control heavy metals in soil
Analysis of the effects of different compound remediation technologies on vegetable biomass
Taking charcoal (Tan), bacteria (Jun) and Sedum plumbizincicola (JT) indicators as fixed factors, soil type (Tu) and vegetable type (ZW) indicators as covariates, and vegetable dry weight and total biomass (vegetables+Sedum plumbizincicola) indicators as dependent variables, carrying out the multivariate general linear model analysis by SPSS19.0 to study the effects of charcoal, bacteria and Sedum plumbizincicola compound technology on the dry weight and total biomass of vegetables.
From the test results of inter-body effect, it can be seen that the significant P values of soil type, vegetable type, different proportion of charcoal, intercropping Sedum plumbizincicola or not and charcoal-bacteria combination on the dry weight of vegetables are all less than 0.05,
indicating that these items have significant effects on the dry weight of vegetables; soil type, vegetable type, different proportion of bacteria, Sedum plumbizincicola or not and bacteria-
Sedum plumbizincicola combination have a significant P value of less than 0.05, indicating that these factors have a significant impact on the total biomass.
Analysis of the effects of each index shows that the charcoal-bacteria combination technology has the greatest impact on vegetable biomass and total biomass, with its effects of 28.45% and 23.82% respectively, followed by the soil type index with its effects of 20% and 13.53% respectively. However, on the whole, the combined technologies of bacteria-Sedum plumbizincicola, charcoal-Sedum plumbizincicola and charcoal-bacteria-Sedum plumbizincicola have little effect on vegetables and total biomass.
Effect quantity analysis of different compound remediation technologies on heavy metal content in vegetables
Taking charcoal (Tan), bacteria (Jun) and Sedum plumbizincicola (JT) indicators as fixed factors, soil type (Tu) and vegetable type (ZW) indicators as covariates, and vegetable heavy metals (Cr, As, Cd, Pb, Hg) as dependent variables, and carrying out the multivariate general linear model analysis by SPSS19.0 to study the effects of charcoal, bacteria and Sedum plumbizincicola compound technology on heavy metals Cr, As, Cd, Pb and Hg in vegetables.
From the test results of inter-body effect, it can be seen that the significant P values of vegetable types, biomass charcoal, Thiobacillus ferrooxidans, charcoal-bacteria, charcoal-
Sedum plumbizincicola and bacteria-Sedum plumbizincicola combination on Cr in vegetables are all less than 0.05, indicating that these items have significant effects on Cr enrichment in vegetables; soil type, biomass charcoal, Thiobacillus ferrooxidans, intercropping Sedum plumbizincicola or not, charcoal-bacteria, charcoal-Sedum plumbizincicola and bacteria-Sedum plumbizincicola combination all have significant P values less than 0.05, indicating that these factors have significant effects on the enrichment of As in vegetables; the significant P values of vegetable types, biomass charcoal, Thiobacillus ferrooxidans, intercropping Sedum plumbizincicola or not and charcoal-bacteria on Cd in vegetables are all less than 0.05, indicating that these factors have significant effects on Cd enrichment in vegetables; the significant P values of soil type, biomass charcoal, Thiobacillus ferrooxidans, intercropping
Sedum plumbizincicola or not, charcoal-bacteria, charcoal-Sedum plumbizincicola and bacteria-
Sedum plumbizincicola combination are all less than 0.05, indicating that these factors have significant effects on Pb enrichment in vegetables; the significant P values of soil type, biomass charcoal, Thiobacillus ferrooxidans, charcoal-bacteria and charcoal-Sedum plumbizincicola on
Hg in vegetables are all less than 0.05, indicating that these factors have significant effects on
Hg enrichment in vegetables.
Through the analysis of the effects of each index, it is found that carbon-bacteria combined technology has the greatest impact on heavy metals Cr, As, Cd, Pb and Hg in vegetables, and the effects are 78%, 74%, 50%, 66% and 54% respectively. Among other combined technologies, the combination of bacteria and Sedum plumbizincicola has a great influence on the Cr of vegetables, with an effective amount of 49%, and the combination of carbon and
Sedum plumbizincicola has a great influence on the Cr, As, Pb and Hg of vegetables. It can be seen that the combined application of several technologies greatly affects the enrichment of heavy metals in vegetables.
Experimental conclusion
Experimental conclusion of combined application of biomass charcoal- Thiobacillus ferrooxidans-Sedum plumbizincicola
Charcoal-bacteria combination technology has the greatest influence on the biomass accumulation and total biomass of vegetables, followed by soil types and vegetable types; charcoal-bacteria combination technology has the greatest influence on total N and total P of vegetables, and biomass charcoal has the greatest influence on total K of vegetables; charcoal- bacteria combination technology has the greatest influence on heavy metals Cr, As, Cd, Pb and
Hg in vegetables; on the whole, the charcoal-bacteria combination technology has a significant impact on many indexes of vegetables, and the charcoal-bacteria combination technology is a suitable compound remediation technology.
It is obvious to those skilled in the art that the present invention is not limited to the details of the above exemplary embodiments, but is implemented in other specific forms without departing from the spirit or essential characteristics of the present invention. Therefore, the embodiments should be regarded as illustrative and non-restrictive in all respects. The scope of the invention is defined by the appended claims rather than the above description, and therefore all changes that fall within the meaning and range of equivalents of the claims are intended to be embraced by the invention. Any reference signs in the claims should not be regarded as limiting the claims involved.

Claims (8)

CONCLUSIESCONCLUSIONS 1. Een inrichting voor het saneren van zware metalen in landbouwgrond door versterking van Sedum plumbizincicola met biohoutskool in combinatie met micro-organismen, welke inrichting een kweekmechanisme (8) en een grondmaalmechanisme (17) omvat, waarbij — een onder gelegen uiteinde van het teeltmechanisme (9) voorzien is van een steunzitting (2) — een boven gelegen uirteinde van de steunzitting (2) is voorzien van kweekgestellen (1) die zich op gelijke afstand van elkaar bevinden; — vier hoeken van elk kweekgestel (1) en vier hoeken van het boven gelegen uiteinde van elke steunzitting (2) zijn voorzien van bevestigingsstaafverbindingsbussen (15), — in de bevestigingsstaafverbindingsbussen (15) bevestigingsstaafgleuven (6) zijn aangebracht; — bevestigingsstaven (3) tussen het kweekgestel (1), de steunzitting (2) en aangrenzende kweekgestellen (1) bevestigingsstangverbindingsbussen aangebracht, — twee lichtgevende lampen (4) symmetrisch aan beide zijden van een onderkant van elk kweekgestel (1) zijn aangebracht, — tussen de aangrenzende lampen (4) watersproeiers (11) zijn aangebracht; — een helderheidssensor (10) aan een zijde van de watersproeier (11) in de middenpositie is aangebracht, — de helderheidssensor (10) elektrisch is verbonden met de lichtgevende lampen (4); — vijf kweekbakken (5) op gelijke afstanden in het boven gelegen uiteinde van de kweekgestellen (1) zijn geplaatst, — de kweekbakken (5) in overeenstemming met de watersproeiers (11) zijn geplaatst; — bodemvochtsensoren (16) in de kweekbakken (5) zijn aangebracht, — een zijde binnen het grondmaalmechanisme (17) vast is voorzien van een vaste verbrijzelplaat (18), en de andere zijde binnen het bodemverbrijzelingsmechanisme (17) verschuifbaar is voorzien van een verschuifbare verbrijzelplaat (19), — tegenoverliggende zijden van het grondmaalmechanisme (17) en de vaste verbrijzelplaat (18) beide zijn voorzien van verbrijzelplaten (20), — de twee verbrijzelingsplaten (20) maasvormig zijn; en — een zeefbak (25) schuin onder de vaste verbrijzelplaat (18) van het grondmaalmechanisme (17) is geplaatst.A device for the remediation of heavy metals in agricultural soil by strengthening Sedum plumbizincicola with biocharcoal in combination with micro-organisms, which device comprises a cultivation mechanism (8) and a soil grinding mechanism (17), wherein - a lower end of the cultivation mechanism (9) is provided with a support seat (2) - an upper end of the support seat (2) is provided with breeding frames (1) which are equidistant from each other; - four corners of each breeding frame (1) and four corners of the upper end of each support seat (2) are provided with mounting rod connecting bushings (15), - mounting rod slots (6) are provided in the mounting rod connecting bushes (15); — fixation rods (3) are mounted between the rearing frame (1), the support seat (2) and adjacent rearing frames (1) mounting rod connecting bushes, — two luminous lamps (4) are arranged symmetrically on both sides of a bottom of each rearing frame (1), — water sprinklers (11) are arranged between the adjacent lamps (4); - a brightness sensor (10) is arranged on one side of the water nozzle (11) in the center position, - the brightness sensor (10) is electrically connected to the luminous lamps (4); - five breeding trays (5) are placed at equal distances in the upper end of the breeding frames (1), - the breeding trays (5) are placed in correspondence with the water nozzles (11); — soil moisture sensors (16) are fitted in the cultivation trays (5), — one side within the soil crushing mechanism (17) is fitted with a fixed crushing plate (18), and the other side is slidably fitted with a sliding plate inside the soil crushing mechanism (17). crushing plate (19), — opposite sides of the ground crushing mechanism (17) and the fixed crushing plate (18) both have crushing plates (20), — the two crushing plates (20) are meshed; and - a screen box (25) is placed obliquely under the fixed crushing plate (18) of the soil crushing mechanism (17). 2. De inrichting voor het saneren van zware metalen in landbouwgrond door versterking van Sedum plumbizincicola met biohoutskool in combinatie met micro-organismen volgens conclusie 1, waarbijThe device for the remediation of heavy metals in agricultural soil by strengthening Sedum plumbizincicola with biocharcoal in combination with microorganisms according to claim 1, wherein — de boven en onder gelegen uiteinden van elke bevestigingsstaaf (3) via klemgroeven vast verbonden zijn met de bevestigingsstaafsleuven (6), — een vooreinde van een zijde van elk kweekgestel (1) voorzien is van een eerste magnetisch blok (7), en — een achterzijde van de andere zijde van het kweekgestel (1) is voorzien van een tweede magnetisch blok (8).- the upper and lower ends of each mounting bar (3) are fixedly connected via clamping grooves to the mounting bar slots (6), - a front end of one side of each breeding frame (1) is provided with a first magnetic block (7), and - a rear side of the other side of the breeding frame (1) is provided with a second magnetic block (8). 3. De inrichting voor het saneren van zware metalen in landbouwgrond door versterking van Sedum plumbizincicola met biohoutskool in combinatie met micro-organismen volgens conclusie 2, waarbij — een voorzijde van de andere zijde van het kweekgestel (1) is voorzien van een eerste magnetische blokgroef (14), — de eerste magneetblokgroef (14) in overeenstemming is met het eerste magneetblok (7); — een achterzijde van een zijde van het kweekgestel (1) is voorzien van een tweede magneetblokgroef (13), en — de tweede magneetblokgroef (13in overeenstemming met het tweede magneetblok (8) is geplaatst.The apparatus for remediation of heavy metals in agricultural soil by strengthening Sedum plumbizincicola with biocharcoal in combination with microorganisms according to claim 2, wherein - a front side of the other side of the breeding frame (1) is provided with a first magnetic block groove (14), - the first magnet block groove (14) is in correspondence with the first magnet block (7); - a rear side of a side of the breeding frame (1) is provided with a second magnet block groove (13), and - the second magnet block groove (13) is placed in correspondence with the second magnet block (8). 4. De inrichting voor het saneren van zware metalen in landbouwgrond door versterking van Sedum plumbizincicola met biohoutskool in combinatie met micro-organismen volgens conclusie 1, waarbij een watertank (12) onder de kweekbakken (5) is aangebracht, en de watertank (12) in verbinding staat met de watersproeiers (11).The apparatus for remediation of heavy metals in agricultural soil by strengthening Sedum plumbizincicola with biocharcoal in combination with microorganisms according to claim 1, wherein a water tank (12) is arranged under the culturing trays (5), and the water tank (12) is connected to the water nozzles (11). 5. De inrichting voor het saneren van zware metalen in landbouwgrond door versterking van Sedum plumbizincicola met biohoutskool in combinatie met micro-organismen volgens conclusie 1, waarbij — de zeefplaat (25) vast verbonden is met een binnenwand van het grondmaalmechanisme (17), — een afvoerband (26) en een materiaalopvangbak (27) onder de zeefplaat (25) zijn aangebracht, — de afvoerband (26) zich aan een zijde van de materiaalopvangbak (27) bevindt; — een filterplaat (30) in de zeefbak (25) is aangebracht; — een groef voor filterstroken (29) is aangebracht binnen een zijde van de filterplaat (30); — de groef voor filterstroken (29) zich boven de afvoerband (26) bevindt, — een ander uiteinde van de filterplaat (30) zich boven het materiaalopvangbak (27) bevindt; — een uiteinde van de zeefbak (25) is voorzien van een zeefbakgestel (28); en — een uiteinde van de zeefbak (25) nabij bij het materiaalopvangbak (27) naar beneden helt.The device for remediation of heavy metals in agricultural soil by strengthening Sedum plumbizincicola with biocharcoal in combination with microorganisms according to claim 1, wherein - the sieve plate (25) is fixedly connected to an inner wall of the soil grinding mechanism (17), - a discharge belt (26) and a material collection container (27) are arranged under the sieve plate (25), - the discharge belt (26) is located on one side of the material collection container (27); — a filter plate (30) is arranged in the sieve tray (25); - a groove for filter strips (29) is arranged inside one side of the filter plate (30); - the groove for filter strips (29) is above the discharge belt (26), - another end of the filter plate (30) is above the material collecting tray (27), - one end of the screen box (25) is provided with a screen box frame (28); and - one end of the screen tray (25) near the material collection tray (27) slopes downwards. 6. De inrichting voor het saneren van zware metalen in landbouwgrond door versterking van Sedum plumbizincicola met biohoutskool in combinatie met micro-organismen volgens conclusie 1, waarbij — een zijde van de verschuifbare breekplaat (19) voorzien is van een breekmotor (22), — een uiteinde van de breekmotor (22) is voorzien van een aandrijfschijf (21), — een drijfstang (23) is aangebracht tussen de aandrijfschijf (21) en de glijdende breekplaat (19), en — twee uiteinden van de overbrengingsstang (23) zijn respectievelijk roterend verbonden met de aandrijfschijf (21) en de glijdende breekplaat (19).The apparatus for remediation of heavy metals in agricultural soil by strengthening Sedum plumbizincicola with biocharcoal in combination with microorganisms according to claim 1, wherein - one side of the sliding rupture disc (19) is provided with a crushing motor (22), - one end of the crushing motor (22) is provided with a driving sheave (21), — a connecting rod (23) is arranged between the driving sheave (21) and the sliding crushing plate (19), and — two ends of the transmission rod (23) are respectively rotatably connected to the driving sheave (21) and the sliding rupture disc (19). 7. De inrichting voor het saneren van zware metalen in landbouwgrond door versterking van Sedum plumbizincicola met biohoutskool in combinatie met micro-organismen volgens conclusie 1, waarbij — de boven en onder gelegen uiteinden van een zijde van de glijdende breekplaat (19) voorzien zijn van schuifblokken (32), — twee zijden van het grondmaalmechanisme (17) symmetrisch zijn voorzien van twee schuifgoten (24), — de schuifgoten (24) schuivend zijn verbonden met de schuifblokken (32}, en — de boven gelegen uiteinden van de vaste breekplaat (18) en de glijdende breekplaat (19) zijn voorzien van materiaalgeleidingsplaten (31).The device for remediation of heavy metals in agricultural soil by strengthening Sedum plumbizincicola with biocharcoal in combination with microorganisms according to claim 1, wherein - the upper and lower ends of one side of the sliding rupture disc (19) are provided with sliding blocks (32), — two sides of the soil crushing mechanism (17) are symmetrically provided with two sliding chutes (24), — the sliding chutes (24) are slidingly connected to the sliding blocks (32}, and — the upper ends of the fixed crushing plate (18) and sliding rupture disc (19) are fitted with material guide plates (31). 8. Een methode voor het bedrijven van de inrichting voor het saneren van zware metalen in landbouwgrond door versterking van Sedum plumbizincicola met biohoutskool in combinatie met micro-organismen volgens willekeurig welke van de conclusies 1 - 7, welke werkwijze de volgende stappen omvat: stap 1:het verzamelen van kalkhoudende grond en gele grond die wijd verspreid is in landbouwgrond als testgrond; stap 2: het selecteren van sla van bladgroenten en waterspinazie van wortelstokken voor het experiment, selecteren van Thiobacillus ferrooxidans met sterke tolerantie voor zware metalen als het test micro-organisme, selecteren van pindaschilstro als biomassa voor houtskool grondstof bij een carbonisatietemperatuur van 350 - 500°C, en het passeren door een 2 mm zeef; stap 3: het verwijderen van stenen en dierlijke en plantaardige resten uit de te testen gele grond en kalkhoudende grond, en deze vervolgens in de schaduw laten drogen; het malen en zeven van de grond door het grondmaalmechanisme (17), waarbij de maalmotor (22) de aandrijfschijf (21) aandrijft om te draaien, en een nok aan het voor gelgen uiteinde van de aandrijfschijf (21) draaiend is verbonden met de overbrengingsstang (23); waarbij wanneer de nok in een cirkel draait, de drijfstang (23) de glijdende breekplaat (19) aandrijft om in het grondmaalmechanisme (17) te schuiven, en de grond door twee maasvormige verbrijzelingsplaten (20) verbrijzelt; waarbij de verbrijzelde grond op de afvoerband (26) door de filterstripgroef (29) valt en de ongefilterde grote gronddeeltjes in de materiaalopvangbak (27) vallen; stap 4: het instellen van biohoutskool op zes niveaus, namelijk 0%, 4%, 6%, 8%, 10% en 20% biohoutskool, kortweg aangeduid als houtskool nul, houtskool één, houtskool twee, houtskool drie, houtskool vier en houtskool vijf; en het vullen van elke pot met 4 kg grond en biohoutskool, waarbij elke pot wordt gevuld met een overeenkomstige hoeveelheid grond en biohoutskool volgens de proefopzet, en het gelijkmatig mengen voor later gebruik; stap 5: het plaatsen van de gemengde grond in de kweekbak (5) van het teeltmechanisme (9), en het kweken van drie groentezaailingen in elke pot; het instellen van Thiobacillus ferrooxidans op zes niveaus, namelijk O ml, 10 ml, 20 ml, 40 ml, 60 ml en 80 ml bacterieoplossingen, waarbij deze kortweg worden aangeduid als bacterie nul, bacterie één, bacterie twee, bacterie drie, bacterie vier en bacterie vijf; overeenkomstig de proefopzet, het aanbrengen van overeenkomstige verhoudingen bacterieoplossingen en drie Sedum plumbizincicola in de pot, het kweken van groentezaailingen en Sedum piumbizincicola door het kweekmechanisme (9), het planten van de groentezaailingen en Sedum plumbizincicola in de grond van de kweekbak (5), het aanpassen van de helderheid van de lichtgevende lamp (4) door de helderheidssensor (10) overeenkomstig de helderheid van het door de plant ontvangen licht om het licht voor de groentezaailingen te verschaffen, het controleren van de bodemvochtigheid door een bodemvochtigheidssensor (16), en het afstellen van de watersproeiers (11) om water te sproeien, en het oogsten van de groenten nadat zij ongeveer 80 dagen gegroeid zijn; en stap 6: het gelijktijdig oogsten van groenten en Sedum plumbizincicola, het wassen en drogen bij 60°C, het malen van groenten en droge monsters van Sedum plumbizincicola voor het testen; het in de schaduw laten drogen van de rhizosfeergrond, zeven en testen.A method of operating the agricultural land heavy metal remediation device by strengthening Sedum plumbizincicola with biocharcoal in combination with microorganisms according to any one of claims 1 to 7, the method comprising the following steps: step 1 : collecting calcareous soil and yellow soil widely distributed in agricultural land as test soil; step 2: selecting lettuce from leafy vegetables and water spinach from rhizomes for the experiment, selecting Thiobacillus ferrooxidans with strong tolerance to heavy metals as the test microorganism, selecting peanut shell straw as biomass for charcoal feedstock at a carbonization temperature of 350 - 500° C, and passing through a 2 mm sieve; step 3: removing stones and animal and vegetable remains from the yellow soil and calcareous soil to be tested, and then drying them in the shade; the grinding and sieving of the soil by the soil grinding mechanism (17), the grinding motor (22) driving the drive disc (21) to rotate, and a cam at the forward end of the drive disc (21) being pivotally connected to the transmission rod (23); wherein when the cam rotates in a circle, the connecting rod (23) drives the sliding crushing plate (19) to slide into the soil crushing mechanism (17), and crushes the soil through two meshed crushing plates (20); wherein the crushed soil falls on the discharge belt (26) through the filter strip groove (29) and the unfiltered large soil particles fall into the material collection bin (27); step 4: setting biocharcoal at six levels, namely 0%, 4%, 6%, 8%, 10% and 20% biocharcoal, referred to as Charcoal Zero, Charcoal One, Charcoal Two, Charcoal Three, Charcoal Four and Charcoal for short five; and filling each jar with 4 kg of soil and biocharcoal, filling each jar with a corresponding amount of soil and biocharcoal according to the experimental design, and mixing evenly for later use; step 5: placing the mixed soil in the cultivating box (5) of the growing mechanism (9), and growing three vegetable seedlings in each pot; adjusting Thiobacillus ferrooxidans to six levels, namely O ml, 10 ml, 20 ml, 40 ml, 60 ml and 80 ml of bacteria solutions, denoting these for short as bacteria zero, bacteria one, bacteria two, bacteria three, bacteria four and bacteria five; according to the experimental design, applying corresponding proportions of bacterial solutions and three Sedum plumbizincicola in the pot, growing vegetable seedlings and Sedum piumbizincicola by the breeding mechanism (9), planting the vegetable seedlings and Sedum plumbizincicola in the soil of the breeding box (5), adjusting the brightness of the luminous lamp (4) by the brightness sensor (10) according to the brightness of the light received by the plant to provide the light for the vegetable seedlings, monitoring the soil moisture by a soil moisture sensor (16), and adjusting the water sprinklers (11) to spray water, and harvesting the vegetables after they have grown for about 80 days; and step 6: harvesting vegetables and Sedum plumbizincicola simultaneously, washing and drying at 60°C, grinding vegetables and dry samples of Sedum plumbizincicola for testing; letting the rhizosphere soil dry in the shade, sieving and testing.
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