MXPA98001258A - Synergistic disinfectant compositions tuberculosis and disinfecc methods - Google Patents

Abstract

The present invention relates to a disinfectant and aqueous cleaning composition, which is a synergistic combination of (a) a sufficient amount of a chlorine-containing bleach compound, such as sodium hypochlorite or sodium dichloroisocyanurate, to provide from about 1100 parts per million by weight of available chlorine level with (b) from about 600 to 800 parts per million by weight of bactericidal quaternary ammonium compounds, such as mixtures of didecyldimethylammonium chloride and chlorides of (C 12 -C 16 alkyl) dimethylbenzylammonium. they are tuberculocides at unexpectedly low concentrations. Also described are two-component compositions and methods for disinfecting surfaces containing the tuberculosis bacillus and other pathogenic microorganisms, such as bacteria and viruses.

Description

SYNERGISTIC DISINFECTANT COMPOSITIONS TUBERCULOSIS AND DISINFECTION METHODS TECHNICAL FIELD This invention relates to aqueous disinfectant and cleaning compositions, which contain a synergistic combination of specific amounts of a chlorine bleach compound with specific amounts of bactericidal quaternary ammonium compounds, wherein the compositions are tuberculocidal at unexpectedly low concentrations, as well as methods for disinfecting surfaces containing the tuberculosis bacillus and other microorganisms such as bacteria and viruses.

BACKGROUND ART The complete elimination of pathogenic microorganisms on various surfaces, especially hard surfaces where such organisms can remain active for relatively long periods of time, has long been an objective of those in charge of cleaning and maintenance in institutional and commercial environments. antiseptic way such as hospitals, medical clinics, meat packing and food preparation areas. A wide variety of chemical disinfecting agents has been developed to meet that objective. However, some of these agents have disadvantages since some are corrosive or of unpleasant odor or capable of staining certain surfaces that commonly need to be cleaned and disinfected. Additionally, many of these agents simply are not effective against certain microorganisms that can be found in institutional environments. The tuberculosis bacillus creates a major problem in institutional and commercial environments, especially in hospitals, due to its tendency to be fairly easily transmitted from one person to another. A number of researchers have reported on the effectiveness of various chemical disinfecting agents to eliminate the tuberculosis bacillus. An article by W.A. Rutala, et al. entitled "Inactivation of Mycobacterium tuberculosis and Mycobacterium bovis by 14 hospital disinfectants "(American Journal of Medicine, vol 91 (3B), pages 267S-271 S (1991)) reports that chlorine dioxide, 0.80% hydrogen peroxide plus 0.06% peroxyacetic acid and one iodophor achieved complete inactivation of both the microorganisms of the titer, while two different quaternary ammonium compounds, as well as 100 parts per million ("ppm") of chlorine were not effective against both microorganisms .They reported that glutaraldehydes, a phenolic and chlorine (1, 000 ppm) were completely effective against Mycobacterium tuberculosis and showed good inactivation of Mycobacterium bovis.This article reports on page 268S that Mycobacterium tuberculosis "was studied because it is a recognized human pathogen that has been associated with infections caused by ineffective disinfection methods or disinfectants ... and [Mycobacterium] bovis was selected because it is the organ required by the AOAC for tuberculocidal activity test ... "An article by M. Best et al. "Efficacies of selected disinfectants against Mycobacterium tuberculosis" (Journal of Clinical Microbiology, vol.28 (10), pages 2234-9 (1990)) reported in the same way that the tested quaternary ammonium compound (0.04% dimethylbenzylammonium chloride) was ineffective against Mycobacterium tuberculosis. He also reported that sodium hypochlorite required a higher concentration (10,000 parts per million "ppm") of available chlorine to reach an effective level of disinfection than sodium dichloroisocyanurate (6,000 ppm). Chlorine bleaches such as aqueous sodium hypochlorite have long been recognized as being effective against all types of microorganisms, provided that the bleach is used in sufficiently high concentrations such as 5,000 ppm (0.5%) of active sodium hypochlorite and higher depending on the microorganism to be eliminated. These types of solutions are recommended for use to disinfect an area where blood or another potentially pathogenic biological contaminant has been spilled or released, and total disinfection is required. At such high levels of sodium hypochlorite, the chlorine smell of the bleach simply makes this agent undesirable for routine cleaning and disinfection of, for example, hospital rooms, where patients remain in the room during and after the cleaning process and disinfection.

A sterilization system based on a chlorine bleach disinfecting agent using two baths in which the articles to be sterilized are placed, is described in U.S. Patent No. 4,418,055 to Andersen et al. In this system, the ingredients used in the sterilization system are stored in hermetically sealed bags that hold reactive ingredients away from each other until use. The bags also provide pre-measured quantities of the ingredients to avoid any error that could be caused by having to distribute the user each of the necessary components. Quaternary ammonium compounds have long been recognized as being useful for their antibacterial properties as can be seen from US Patent Nos. 3,836,669 to Dadekian; 4, 320, 147 for Schaeufele; 4, 336, 151 for Like et al.; 4,444,790 for Green et al.; 4,464, 398 for Scheets et al.; and 4,540,505 for Frazier. Higher levels of quaternary ammonium compounds have been reported to be effective disinfectants against several pathogenic microorganisms, even including Mycobacterium tuberculosis. J. Dos Reis Meirelles Neto et al. reported in their paper entitled "Tuberculocidal activity of some cationic detergents" ("Tuberculocidal activity of some cationic detergents") - Folha Med., vol. 87 (4), pages 227-232 (1983) - that the quaternary ammonium salts of benzalkonium chloride, ammonium-alkyl-dimethylbenzyl chloride plus ammonium-alkyl-dimethylethyl-benzyl chloride and cetylpyridinium chloride in a concentration of 0.4 % showed microbicidal action for sputum microbial flora and some toxic effect for Mycobacterium tuberculosis. Another article by L Szymaczek-Meyer et al entitled "Effect of some disinfectants (phenol derivatives, quaternary ammonium compounds, aldehydes and cloramine) on human type tubercle bacilli sensitive and resistant to antibacillary drugs" (Med. Dosw. Mikrobiol, vol.31 ( 1), pages 53-59 (1979)) reported the results of using commercial concentrations of disinfectants for hospitals available in dense suspensions of tuberculosis bacillus Hsub 3sub 7Rv and natural species of human type sensitive and resistant to antibacillary drugs They found that some of the disinfectants verified were tuberculocides, but the solutions generally used containing at least approximately 0 6% of active disinfecting agent To minimize the expense, unpleasant odors and possible harmful effects of disinfecting agents on surfaces to be disinfected, it is convenient to minimize the amount of disinfectants used at the same time the efficacy against pathogenic microorganisms is still retained, especially against the tuberculosis bacillus such as Mycobacterium tuberculosis. As will be explained in more detail below, I have found that a combination of a specific amount of a chlorine bleach compound such as dichlorosiocyanurate sodium with a specific amount of a bactericidal quaternary ammonium compound, provides a composition that is effective against the tuberculosis bacillus even when the concentration of each compound used, when evaluated individually at that concentration is ineffective against the tuberculosis bacillus. In the past, combinations of chlorine and peroxygen bleaches with quaternary ammonium compounds have been taught, but for different purposes or at different levels of use than I have discovered. Typically, a relatively large amount of chlorine bleach (0.5% to 1% or more of active bleaching compound, which is 5,000 to 10,000 ppm of active bleaching compound) has been combined with cationic surfactants (which typically refer to quaternary ammonium generally and not all these compounds have disinfectant or bactericidal properties). If a sufficiently high amount of bleaching compound is used (ie, which, by itself, is capable of destroying pathogenic microorganisms, including tuberculosis bacillus), then there is no need to include a bactericidal quaternary ammonium compound for disinfection purposes. U.S. Patent No. 3,669,891 to Greenwood et al. Shows various compositions that emit visible light during use. Example 8 shows a two component air / germicidal refreshing composition, comprising a combination of an aqueous sodium hypochlorite solution (0.5% available chlorine) and a second solution containing 1% cetyltrimethylammonium bromide and 1. 5% oleyl di-betahydroxyethyl methylammonium methosulfate. The two solutions were combined in the nozzle of a two-compartment pump atomizer package set up to deliver equal amounts of liquid from each compartment. Thus, the level of all the quaternary ammonium compounds alone in the mixed liquids was 0 75% or 7,500 ppm of active material. US Pat. No. 2,987,435 to Davies et al., Shows synergistically active germicidal methods and materials for cleaning and disinfecting materials by combining a hypochlorite with a quaternary ammonium compound One such method involves contacting a surface with a composition containing sodium hypochlo- prate at an available chlorine level of 0 45% to 5%, followed by contacting the surface with an aqueous solution of benzalkonium chloride (0 05% to 5 0% by weight, preferably 0 02%) Another method involves applying a solution comprising about 0 45% to about 5% by weight of available chlorine and from about 0 5% to 5% by weight. % of benzalkonium chloride Davies et al show that the active materials are added so that the composition contains the equivalent of at least 0 1% in available chlorine weight and 0.01% by weight of quaternary ammonium salt Preferably, the compositions contain from 0.1% to 5% by weight of available chlorine and from 0.05 to 20% of quaternary ammonium salt are incorporated by each part by weight of chlorine Davies et al. show that a concentrated aqueous germicidal solution can be made, this is a solution of aqueous sodium hypochlo- prate solution containing from 0 25% up to 1 5% of available chlorine, to which is added from 2 5% to 7 5% of a quaternary ammonium salt to form a concentrate, and then the concentrate is diluted 40 times to obtain a germicidal bleaching solution. U.S. Patent No. 3,852,210 to Krezanoski shows a stable liquid detergent containing active oxygen for use as a composition. bleaching and cleaning, particularly for the skin This contains from 0 1% up to 50% of soluble peroxygen compounds in the detergent vehicle as the in active ingredient, and optionally, from 0 01% to 10% of a quaternary ammonium surfactant agent. The latter is added for the purpose of increasing the detergent power and stability of the foam, as well as for imparting residual germicidal activity. No. 4 169,065 for Robertson shows a cleaning mixture for canine ears containing a mixture of alcohol, acetic acid, hydrogen peroxide (0 3% to 3%), benzethonium chloride (soap) (0 01% to 0 06% ), and water None of these patents suggest the use of chlorine bleach cleaning compositions, which could contain cleaning compounds plus organosilicon quaternary ammonium compounds (to provide residual antibactepane activity to surfaces cleaned with such compositions) are shown in U.S. Patent Nos. 4, 783, 281 to Stoddart (alkali metal hypochlorate level from 1% to 12%), 4,576, 728 to Stoddart (hypo level) alkali metal oclopto from 1% to 12%), and 4,005,028 for Heckert et al (any chlorine-producing bleach where the available chlorine content of the cleaning composition varies from 0 5% to 10%) These patents do not suggest the use of organic quaternary ammonium compounds that are free of silicon Stable or perfumed stable chlorine bleach compositions, which may contain quaternary ammonium compounds are known. U.S. Patent Nos. 4,136,645 to DeSimone shows aqueous home bleach compositions containing from 5% to 15% sodium hypochlocyte together with 0.025% to 1% (based on the weight of the aqueous bleach composition) of a salt of quaternary ammonium where the latter is to help disperse a perfume oil within the bleach composition to mask the odor of the home bleach composition. U.S. Patent No. 5,080,826 to Colborn et al. shows stable fragrance home bleach compositions, in US Pat. which an immiscible or slightly miscible fragrance is dispersed in the bleach without wetting the inside walls of the plastic package of the bleach Colborn et al. show that 0-100 ppm of various surfactants can be used to disperse the fragrance within the bleach composition, where such surfactants include quaternary ammonium compounds British Patent No. 1, 466, 5 60 for Jeyes Group Ltd shows aqueous thickened solutions of alkali metal hypochlorites (1% to 14% available chlorine) to be used for cleaning and disinfecting various surfaces, especially vertical surfaces A combination of an alkaline metal sarcosinate surfactant with another surfactant that may include quaternary ammonium compounds are useful as thickening agents British Patent No. 1, 548,379 to Jeyes Group Ltd is similar to the '560 patent, except that it uses a thickening agent for aqueous solutions of alkali metal hypochlorites of a sucrose surfactant and one or more other surfactants that are soluble in the hypochlorite, including quaternary ammonium compounds. US Patent No. 4,264,466 to Carleton et al. Shows humus consisting of a liquid phase and a dispersed solid phase. Humus can be used as a detergent and can include water-sensitive detergent auxiliaries such as bleaches, as well as cationic surfactants. some of the cationics are capable of providing sanitization of the wash load, but are mainly useful as suspending agents for the dispersed solids. U.S. Patent No. 4,461, 652 to Richmond shows a process and product for removing barnacles from marine vessels, which is composed of a base mixture of (1) 15% to 35% liquid hydrocarbon, (2) 15% to 6% surfactant which may include the dialkylbenzylammonium salt, alkyl of (5) below, (3) 0% to 2% alcohol (4) 0 5% to 5% metal hypochlorite, (5) 0 5 to 1 5% alkyl dialkylbenzylammon salt, (6) 305 to 50% water, and 52 5% to 0 5% of carriers ine The base mix can be diluted substantially 1 1 with water before use SUMMARY OF THE DESCRIPTION OF THE INVENTION An objective of this invention is to provide disinfectant compositions that can be used in a method for disinfecting vain surfaces to inactivate the tuberculosis bacillus. Another objective of this invention is to provide such compositions that can remain stable and effective during extended periods of time, because the active ingredients are separated from each other until use. A particularly advantageous objective of this invention is to provide compositions that can be used in institutional and commercial environments for cleaning and disinfection purposes, which are more tolerable for people who remain in the disinfected area because the odor of the compositions is much less than if more highly concentrated hypochlorite bleach compositions will be used as the active disinfectant. Still another objective is to provide cleaning compositions and disinfectants which are capable of inactivating the tuberculosis bacillus, but which do not have high concentrations of disinfectant compounds and therefore, are less prone to corrode or stain surfaces to be cleaned and disinfected. These and other objects of the present invention are provided by a method for disinfecting a surface containing the tuberculosis bacillus comprising applying to said surface an aqueous composition comprising, disinfecting agents, an amount of a chlorine-containing bleaching compound such as sodium hypochlorite or sodium dichlorosiocyanurate, effective to provide from about 1, 100 to about 2,500, more preferably from about 1, 100 to about 2,200, parts per million by weight of available chlorine level, and from about 600 to about 800, more preferably from about 650 to about 700, parts per million by weight of a bactericidal organic quaternary ammonium compound, such as a mixture of didecyldimethylammonium chloride and (C 2 -C 6? alkyl) dimethylbenzylammonium chlorides. This invention also relates to a composition for disinfecting a surface containing the tuberculosis bacillus comprising an aqueous composition comprising, as disinfecting agents, an amount of chlorine-containing bleach compound such as sodium hypochlorite or effective sodium dichloroisocyanurate to provide from about 1.100 to about 2,500 parts per million by weight of available chlorine level and from about 600 to about 800 parts per million by weight of a bactericidal organic quaternary ammonium compound.

BEST MODE FOR CARRYING OUT THE INVENTION In order to obtain adequate disinfection of surfaces containing the tuberculosis bacillus, two compounds are required to be present in the aqueous disinfectant compositions of the present invention. The first required ingredient is a chlorine-containing bleach compound of the type that is well known and from which many are commercially available. In an aqueous medium, such compounds are sufficiently dispersible in water to provide active chlorine-based species that have a detrimental effect on pathogenic microorganisms, as well as possessing the properties of being able to "whiten" by converting the materials they are put into. colorless or white contact.

Examples of such chlorine-containing bleach compounds are those that produce an effective amount of hypochlorite species in aqueous solution, which is sufficient to be useful in the compositions of the present invention. The determination of which of such compounds are useful in the present invention can be readily determined by dissolving the compound in water and measuring the level of active chlorine in the aqueous solution. Such compounds may include alkali metal hypochlorites, alkaline earth metal hypochlorites, hypochlorite addition compounds, chloramines, chlorimines, chloramides, chlorimides and mixtures thereof. Specific examples of such compounds include sodium hypochlorite, potassium hypochlorite, monobasic calcium hypochlorite, dibasic magnesium hypochlorite, chlorinated trisodium phosphate dodecahydrate, trichloryenuric acid, 1,3-dichloro-5,5-dimethylhydantoinium, N-chlorosulfamide, chloramine T (para-toluenesulfoncloramide sodium available from RW Greeff & amp; amp;; Company of Green ich, Connecticut), dicloramine T (N, N-dichloro-p-toluenesulfonamide), chloramine B (sodium benzenesulfurloramide) and dichloramine B. Currently, the preferred bleaching compounds are alkali metal hypochlorites and alkali metal dichloroisocyanurates. The most preferred of these compounds are sodium hypochlorite and sodium dichloroisocyanurate, since commercial quantities of such compounds are readily available. The bleaching compounds are present in amounts that are effective to provide from about 1, 100 to about 2,500 parts per million by weight of available chlorine level based on the total weight of the composition, with from about 1.1 to about 2,200 ppm. being more preferred. Less than about 1, 100 ppm of available chlorine level of bleaching compound results in compositions which are not completely effective against the tuberculosis bacillus and more than about 2,500 ppm of available chlorine level of bleaching compound tend to provide disinfectant compositions that They have strong odors of chlorine and hypochlorite, as well as being more corrosive to the surfaces being disinfected. The "level of available chlorine" can be determined by well-known methods, such as using the aqueous solution containing active chlorine with thiosulfate. of sodium to obtain the concentration of available chlorine as sodium hypochlorite, using well-known analytical methods. The organic quaternary ammonium compounds useful in the present invention are those which have bactericidal properties and which are relatively soluble in aqueous medium. These compounds are also well known as can be seen from US Pat. Nos. 3,836,669 to Dadekian, 4,320, 147 to Schaeufele; 4, 336, 151 for Like et al, 4,444,790 for Green et al, 4 464,398 for Sheets et al. , and 4,540,505 for Frazier mentioned above. Many are commercially available materials sold under the tradenames Bardac®, Barquat® and Hyamine® by Lonza, Ine of Fairlawn, New Jersey, BTC by Stepan Company of Northfield I Illinois and Maquat® by Mason Chemical Company of Arlington Heights, Illinois Examples of such compounds are di (C8-C? 2 alkyl) di (C? -C4 alkyl) ammonium salts, where the salts include halides, sulfates, and methosulfates such as dioctyl dimethyl ammonium chloride, didecyldimethylammonium chloride , didodecyldimethylammonium chloride, didecyldimethylammonium bromide, didecyldimethylammonium sulfate, didecyldimethylammonium methosulfate, or dioctyl diethylammonium chloride; salts of (d2-C18 alkyl) di (C1-C4 alkyl) benzylammonium where the salts include halides, sulfates and methosulfates such as chloride, bromide, sulfate or (C-? 2-C? 6 alkyl) dimethylbenzylammonium methosulfate; and ((alkyl) di (C -? - C4 alkyl) ethylbenzylammonium salts where the salts include halides, sulfates and methosulfates, such as (C? 2-Cie alkyl) dimethylethylbenzylammonium chloride, bromide, sulfate or methosulfate, and mixtures of As is well known, the manufacturing processes used to prepare such compounds frequently result in mixtures of variant alkyl chain length compounds, and thus, the designation "C? 2-C? 8" is used to describe such Currently, the preferred quaternary ammonium compounds are a mixture of didecyldimethylammonium chloride and (C12-C6 alkyl) dimethylbenzylammonium chloride, more preferably in a proportion of 51.5: 48.5 by weight.The quaternary ammonium compounds are present in the composition in amounts ranging from about 600 to about 800 parts per million by weight of the total composition, with from about 650 to about 700 ppm being more preferred. s of approximately 600 ppm of quaternary ammonium compound results in compositions, which are not completely effective against the tuberculosis bacillus and more than about 800 ppm of quaternary ammonium compound tends to be wasted and introduces more risk that the quaternary ammonium will stain surfaces to be disinfected. The above compounds are dissolved in water to form the disinfectant compositions of the present invention. As generally used for cleaning and disinfecting compositions, small amounts, generally less than about 5% by weight of the total composition, of various water soluble or miscible solvents, such as lower alcohols such as ethanol and isopropanol, glycol ethers and the like can also be included to improve the cleaning efficiency or wettability of the surface of the compositions of the present invention. The compositions of the present invention may also contain various conventional detergents or surfactants to assist in cleaning properties and surface wetting are compatible with the bleach and quaternary ammonium compounds. Generally, the amounts of such surfactants or detergents do not exceed about 15% by weight of the total weight of the composition. Such surfactants or detergents include nonionic, amphoteric, zwitterionic and cationic surfactants. Generally, anionic surfactants tend to react with cationic materials such as quaternary ammonium compounds and if any anionic surfactant will be included, it should not interfere with the tuberculocidal disinfecting properties of the quaternary ammonium compounds to any significant degree. Nonionic surfactants such as alkyl amine oxides are currently preferred if such surfactants or detergents will be included in the compositions of the present invention. Formulating salts and chelating agents of the type conventionally used in liquid detergent compositions for hard surface cleaning may also be included. in the compositions of the present invention in small amounts, generally less than about 5%, provided they do not interfere with the disinfecting properties of the composition. Such forming salts include sodium sesquicarbonate, sodium carbonate, sodium borate, potassium carbonate, pyrophosphate tetrapotasium, sodium metasilicate and the like. Chelating agents may include water-soluble chelating agents such as alkali metals or substituted ammonium polycarboxylates, such as sodium or potassium salts of ethylenediaminetetraacetic acid ("EDTA"), such as tetrahedral EDTA. Other conventional additives, which do not interfere with the disinfecting properties of the compositions and which are compatible with the other ingredients present in the composition, can be included in minor amounts of not more than about 5% by weight of the total composition such as colorants. , perfumes, and ultraviolet light stabilizers INDUSTRIAL APPLICABILITY The compositions of the present invention are simple to produce and use since the bleaching compound may tend to lose some of its ability to provide active chlorine species to eliminate the tuberculosis bacillus, which is known to be difficult to activate. Thus, it is more preferred to separate the bleaching compound from the quaternary ammonium compound until such time as the composition will be used. In one embodiment, all of the ingredients to be used in the composition can be mixed together with the exception of the bleaching compound to form a component. The bleaching compound may take the form of an aqueous solution of, for example, sodium hypochlo- tote, as a second component having sufficient sodium hypochlo- phate level to provide the desired level of available chlorine when the first component is mixed with a preselected amount. of the aqueous solution of sodium hypochlocite (second com rapporteur) The mixture of these two components can be done just before application to a surface containing the tuberculosis bacillus can be done in vain ways US Patent No. 4,418, 055 to Andersen et al mentioned above provides a way by which this it can be done simply by sealing the correct amounts of each of the two components in separate bags, which are then opened and mixed together briefly before application. Other possible ways are to place the disinfectant compositions to be separated, particularly where the bleaching compound and other ingredients are solid, in separate sheets which can be dissolved in water such as those described in US Patent No. 4,797,221 for Gueldenzopf and are simply emptied into a container with water and allowed to dissolve until the contents they are well mixed, thus generating the compositions disinfect of the present invention. U.S. Patent No. 3,669, 891 to Greenwood et al. describes another way by which two components could be mixed, which is to place each component in a two-compartment trigger atomizer bottle. When the trigger is stopped pressing, preselected portions of each component are dragged to the head of the atomizer, mixed together and sprayed on the surface to be cleaned and disinfected. In a less preferred embodiment, all of the ingredients can be mixed together and the resulting disinfectant composition can be stored until use. The level of available chlorine from the stored disinfectant composition should be monitored to ensure that a sufficient level of available chlorine is present before use on disinfectant surfaces containing the tuberculosis bacillus. In this manner, the compositions can be applied to surfaces to be disinfected in a variety of ways such as by sponge, atomization, mopping, rag rinsing, foaming, submersion and in several different ways that are commonly used for conventional disinfecting agents. As noted in the newspaper article by Rutala et al. mentioned above, compositions that are tuberculocidal tend to be very effective and inactivate lipophilic and hydrophilic viruses, yeasts, fungi and bacteria. Thus, the compositions of the present invention will find use as multipurpose disinfectants for many surfaces that need disinfection such as countertop surfaces, work areas, bathrooms, meat packing rooms, food handling areas and the like. The following Examples are provided to show various aspects of the present invention without departing from the scope and spirit of the invention. Unless indicated otherwise, all parts and percentages used in the following Examples are by weight. "Ambient temperature" is approximately 20 ° C. The milliliters are expressed as "ml", the liters are "I", the grams are "g", and the millimeters are "mm". In the following Examples, the effectiveness of various compositions against the tuberculosis bacillus was evaluated by an independent laboratory using standard industry methods with certain modifications. In the Examples, a series of evaluations was conducted according to Official Methods of Analysis of the AOAC, fourteenth edition, 1984, Chapter 4 - Disinfectants, paragraphs 4.039-4.041. "Confirmative In Vitro Test for Determining Tuberculocidal Activity" with the following three modifications to adapt the procedure for the facilities of the independent laboratory using Mycobacterium Bovis (BCG) ATCC 35743, which was exposed to each solution for a period of ten minutes at 20 ° C followed by a 90-day incubation period at 37 ° C ("TB TEST 1"): Modification (1) The subculture used for each test was not agitated during the period of agitation.

Modification (2) The glass culture tubes were of a smaller size than the one specified in the method. The volume of medium added to these smaller tubes was also reduced. This smaller size was selected based on the availability of disposable glassware. Disposable glassware was used to avoid potential problems with accumulation of waste in the glassware. Middlebrook 7H9 Broth Difco B and Medium Kirchners were added in 1 3.5 ml to 16 X 150 mm tubes instead of 18 ml added to 25 mm diameter tubes. The amount of Middlebrook ADC Enrichment added to each tube was 1.5 ml instead of 2.0 ml. Modification (3) To establish effectiveness in the presence of an organic soil load, the method was modified to include a 5% organic soil load. This was incorporated in the method to the additional 1 ml of sterile horse blood serum to 19 ml of the test organism culture used to soak the carriers. Quantitative determinations of the microbial concentration of untreated control carriers both before and after drying were made. In some of the Examples herein, the effectiveness of various compositions against the tuberculosis bacillus were evaluated by an independent laboratory using a second method that followed the method described in Official Methods of Analysis of the AOAC, Fifteenth Edition, 1990, Chapter 6 - Disinfectants, Section 96512D-F. "Confirmative In Vitro Test for Determining Tuberculocidal Activity" using Mycobacterium Bovis (BCG) from Bionetics Research, I nc. (AKZO Teknika Corporation), which was exposed to each solution for a period of ten minutes at 20 ° C followed by an incubation period of 60 days at 37 ° C using the same Modifications (1), (2) and (3) ) for TEST METHOD 1, earlier ("TB TEST 2"). Some of the Examples used a modification of TB TEST 2, where the only modifications of the published method were Modifications (1) and (2) for TEST METHOD 1, above ("TB TEST 3"). Some OF THE EXAMPLES USED a modification of TB TEST 2, where the only modifications of the published method were Modifications (1) and (3) for the PRU EBA 1 METHOD, above ("TB TEST 4"). In the following Examples, the level of available chlorine was determined just before the AOAC Confirmatory Tuberculocidal Tests were conducted. This determination was made using the iodometric method for the evaluation of NaOCI (titration of available chlorine using aqueous sodium thiosulfate for an iodine endpoint) using the Iodometric Method I described in Section 408A of the sixteenth edition of Standard Methods for the Examination of Water and Wastewater, American Public Health Association, 1995 ("METHOD OF TITU LATION YODOMETR ICA"). The available chlorine as sodium hypochlorite in parts per million of the solution was obtained by multiplying the milliliters of sodium thiosulfate used in the titration of the sample by 35.46 (available chlorine factor) and dividing that by the amount in grams of the sample used.

The following ingredients were used in the compositions described in the Examples: Ammonium hydroxide (28%) - ammonium hydroxide solution at a 25% ammonia concentration. Benzalkonium chloride (50%) - solution of n-alkyl dimethyl benzylammonium chloride at an active concentration of 50% - Barquat® MB-50 from Lonza, Inc. Citric acid (50%) - aqueous solution of citric acid to a Active concentration of 50% Caustic soda (50%) - sodium hydroxide solution at an active concentration of 50% Cocamide DEA - coconut acid ethanolamide mixture (Clindrol® CGN from Clmtwood chemical Company of Chicago, IL) didecyldimony chloride (50%) - didecyl dimethylammonium chloride solution at an active concentration of 50% didecyldimonium chloride (80%) - solution of didecyldimethylammonium chloride at an active concentration of 80% - Bardac ® 22 of Lonza Ine Hydroxyethylcellulose - Natrosol ® 250H of Aqualon of Wilmington, DE KATHON® LX (14%) - 5-chloro-2-met? L-4-? Sot? Aolol-3-one of Rohm and Haas Company of Philadelphia, PA at an active concentration of 14% CLOTHING EARNER - Clorox® Bleach standard household laundry bleach from The Clorox Company of Oakiand, CA containing approximately an active chlorine level of 5 25% Lauramine Oxide (30 %) - lauryl dimethyl ammonium chloride solution at an active concentration of 30%. Myristalconium chloride and Quaternium 14 (50%) - myristyldimethylbenzylammonium chloride and dodecyl dimethylethylbenzylammonium chloride at an active concentration of 50% (BTC® 2125 from Stepan Company of Northfield, I L. Nonoxynol-9-polyoxyethylene (9.5) nonyl phenyl ether Octoxynol-9-polyoxyethylene (9) octyl phenyl ether (Triton® X-100 CG from Union Carbide Corporation of Danbury, CT) Octoxynol-13-polyethylene glycol (13) octyl phenyl ether-Igepal® CO-720 from GAF Chemicals Corporation of Wayne, NJ Pareth 15-9 - polyethylene glycol ether from a mixture of synthetic Cn-C 5 fatty alcohols with an average of 9 moles of ethylene oxide - Tergitol® 1 5-S-9 from Union Carbide Corporation from Danbury, CT Polyquaternium 10 - polymeric quaternary ammonium salt of hydroxyethylcellulose treated with a substituted trimethylammonium epoxide (Ucare® Polymer J R-400 from Amerchol Corporation of Edison, NJ TABLETA SDI - effervescent sodium dichloroisocyanate tablet 3.5 g sold under the trade name of CDB Sani Fizz 50 LT by Olin Corporation of Chesire, CT. (U.S. EPA Reg. No. 1258-1 160). It was found that one such tablet provides approximately 275 ppm of active chlorine level upon dissolution in 3.79 I of water. Sodium silicate (50%) - aqueous sodium silicate solution filtered at an active concentration of 50%. Sodium metasilicate - sodium metasilicate pentahydrate.

Sodium tppoliphosphate - anhydrous sodium tppo phosphate T-EDTA (40%) - aqueous solution of tetrasodium salt of ethylenediaminetetraacetic acid at an active concentration of 40% TK-EDTA (50%) - tppotasium salt of ethylenediaminetetraacetic acid at a concentration active 50% TKPP - tetrapotasium pyrophosphate, anhydrous It is quite common to provide cleaning compositions and disinfectants in concentrated forms which are intended to be diluted at the time of use. In the Examples, the following concentrated compositions were diluted with water just prior to the CONC AA evaluation. enough water taken to obtain 100 parts by weight of concentrate, 0.005 parts of dye, 4 parts of T-EDTA (40%), 1 part of sodium sesquicarbonate and 4 parts of Lauramine Oxide (30%) were added with stirring. , 0 3 parts of fragrance, 10 88 parts of Didecyldimonium Chloride (80%), and 16 83 parts of Benzalkonium Chloride (50%) CONC B 1 5 parts of Octox? Nol-13 and 1 part of fragrance are mixed to form a premix. The following ingredients were then mixed together with 83 721 parts of water taken in 2 parts of Citric Acid (50%), 1 part of sodium citrate, 10 parts of Nonox-nol-9, 0 004 parts of KATHON® LX (14%), and 0 025 parts of dyes The premix was then mixed into the mixture with stirring. A batch of CONC B was found to have a pH value of about 4 1 1 CONC C 1 5 parts of Octox-nol-13 and part of fragrance were mixed to form a premix The following ingredients were then mixed together with 86 96 parts of water taken or 5 parts of citric acid, 10 parts of Nonox-nol-9, and 0,004 parts of KATHON® LX (14%) The premix was then mixed into the mixture with stirring. A batch of CONC C was found to be a clear solution with a decrease having had a pH value of 2 50 CONC. 100 part by weight of concentrate, add stir with 0 5 parts of a 1% solution of dye, 4 parts of T-EDTA (40%), 1 part of sodium sesquicarbonate, 4 parts of Lauramma Oxide (30%), 0 3 parts of fragrance, 10 88 parts of Dideldimonium Chloride (80%), and 16.38 parts of Benzalkonium Chloride (50%) CONC E The following was mixed with stirring in the given order, and then agitation was allowed for one to two hours until the solution became clear 63 5 parts of tap water, 9 parts of T-EDTA, 8 75 parts of Benzalkonium Chloride (50%), 8 75 parts of Didecyldimonium Chloride (50%), 7 parts of Pareth 15-9, and 3 parts of sodium sesquicarbonate CONC F A small amount of water was mixed with 0 005 parts of two dyes to form a paste Taking the amount of water used to form the paste under consideration, a sufficient amount of water to form a total of 100 parts by weight of concentrate was charged into a mixing tank The paste was added then with agitation to the mixing tank Subsequently, the following ingredients were added to the mixing tank in the order given 1 8 parts of Caustic Soda (50%), 4 0 parts of Sodium Silicate (50%), 1 5 parts of TK -EDTA (50%), 3 2 parts of Sodium Phospholiphosphate, 3 parts of Nonox? Nol-9, 3 parts of Benzalkonium Chloride (50%), and 0 05 parts of perfume CONC G This concentrate was produced Mix the following ingredients 1872 28 parts of deionized water, 1 50 parts of hydroxyethylcellulose, 2 00 parts of Polyquaternium 10, 45 parts of dipropylene glycol monomethyl ether, 4 62 parts of Sodium Metasilicate 32 02 parts of TKPP, 18 parts of 98 parts of octox? nol-9, 2 00 parts of Cocamid a DEA, 3 80 fragrance parts, 8 54 parts of Mipstalconium Chloride and Quaternium 14 (50%), and 9 26 parts of Ammonium Hydroxide (28%) CONC D 1 128 7 8 ml of CONC A were mixed with enough AOAC HARD WATER to make 1, 000 ml of CONC D 1 256 1 95 ml CONC D solution was mixed with enough AOAC HARD WATER to make 500 ml of CONC E 1 100 2 solution 5 ml of CONC E were mixed with enough sterile purified water to make 250 ml of CONC F solution 1 10 2 5 ml of CONC F were mixed with enough AG UA DU RA of AOAC to make 250 ml of solution BLANQU EADOR OF CLOTHES 1 10 - 20 ml of BLANQU EADOR OF CLOTHES were mixed with sufficient sterile purified water to make 200 ml of solution WHITENER OF CLOTHES 1: 20 - 10 ml of CLOTHING BLEACHER they were mixed with sufficient sterile purified water to make 200 ml of solution. BLANQU EADOR OF CLOTHES 1: 35 - 5.71 ml of BLANQU EADOR OF CLOTHES were mixed with purified sterile water sufficient to make 200 ml of solution. CLOTHING WHITENER 1: 45 - 4.44 ml of CLOTHING BLANKET were mixed with sufficient sterile purified water to make 200 ml of solution. To evaluate the efficacy of compositions against the tuberculosis bacillus under common conditions of use that normally involve the use of tap water containing some degree of dissolved minerals as a diluent for concentrates, the concentrated compositions used in the Examples were diluted with Synthetic Hard Water of AOAC containing 400 ppm of CaCO3 ("HARD WATER of AOAC").

Examples 1-6 These Examples illustrate various application and comparative compositions, which were evaluated for their effectiveness against the tuberculosis bacillus. Several disinfectant compositions were evaluated, some of which also contained surfactants, different cleaning agents and additives as well as quaternary ammonium compounds and compounds that provide active chlorine. In some of these and the following Examples, a concentrate was diluted on a volume by volume basis with AOAC WATER DU RA and, if applicable, a bleach compound that provides active chlorine was then added. In other Examples, a bleaching compound was mixed with HARD WATER of AOAC to obtain a disinfectant composition. Table I lists the total calculated levels of both quaternary ammonium compounds and the active chlorine level for each diluted disinfectant composition. The results of evaluating the disinfectant compositions diluted by TB TEST 1, TB TEST 2 and TB TEST 3 routes are reported in Table I. The diluted disinfectant composition of Comparative Example 1A was prepared by mixing 59.1 ml of CONC. A with enough HARD WATER from AOAC to obtain 3.79 I of composition. Comparative Example 1 B was prepared in the same manner as Example 1A, except that 1 18 ml of CONC was used. A. Comparative Example 2A was prepared by mixing 14.8 ml of CONC. B with enough AG UA DU RA from AOAC to obtain 3.79 I of composition. Then, four SDI TABLETS were added to that composition and allowed to disperse completely. Comparative Example 2B was prepared in the same manner as Example 2A, except that eight SDI TABLETS were added. Comparative Example 3 was prepared by dissolving a sufficient number of SDI TABLETS in HARD WATER from AOAC to obtain an active chlorine level of 51 06.2 ppm. Comparative Example 4 was prepared by mixing a portion of CLOTHING WHITENER with AGUA DU RA of AOAC, in a volume: volume ratio of 1: 10 to obtain an active chlorine level of 5106.2 ppm. Comparative Example 5A was prepared by mixing 59.1 ml of CONC. A with enough AOAC HARD WATER to obtain 15.1 I of composition. Then an SDI TABLET was added to the resulting composition and allowed to disperse completely. Comparative Example 5B was prepared by mixing 29.6 ml of CONC. A with enough HARD WATER from AOAC to obtain 7.6 I of composition. Then an SDI TABLET was added to the resulting composition and allowed to disperse completely. Comparative Example 5C was prepared by mixing 14.8 ml of CONC A with enough HARD AOAC WATER to obtain 3.79 I of composition. Then an SDI TABLET was added to the resulting composition and allowed to disperse completely. Application Example 5D was prepared by mixing 14.8 ml of CONC A with enough AGUA DU RA from AOAC to obtain 3.79 I of composition. Then four SDI TABLETS were added to the resulting composition and allowed to disperse completely. Application Example 5E was prepared by diluting 14.8 ml of CONC. A with enough HARD WATER from AOAC to obtain 3.79 I of composition. Then eight SDI TABLETS were added to the resulting composition and allowed to disperse completely. Comparative Example 6A was prepared by diluting 237 ml of CONC. C with enough WATER DU RA of AOAC to obtain 15. 1 I of composition.

Then an SDI TABLET was added to the resulting composition and allowed to disperse completely. Comparative Example 6B was prepared by diluting 18 ml of CONC. C with enough HARD WATER from AOAC to obtain 7.6 I of composition. Then an SDI TABLET was added to the resulting composition and allowed to disperse completely. Comparative Example 6C was prepared by diluting 59.1 ml of CONC. C with enough HARD WATER from AOAC to obtain 3.79 I of composition. Then an SDI TABLET was added to the resulting composition and allowed to disperse completely.

Table I 1 Total amount of quaternary ammonium present Comparative Examples 1 A and 1 B proved the use of relatively high levels of quaternary ammonium compound in a disinfectant cleaning formulation, but no chlorine was added. These compositions were not effective against the tuberculosis bacillus according to TB TEST 1 Examples comparative 2A and 2B show that the addition of 1 100 ppm and 2200 ppm of bleaching compound to a cleaning formulation was insufficient to make the composition tuberculocide according to TB TEST 1. Comparative Examples 3 and 4 verify literature reports that a level of about 5000 ppm of active chlorine level of bleaching compounds such as sodium hypochlorite and sodium dichloroisocyanate produces disinfectant compositions that are tuberculocidal. The results of Example 4 are somewhat contrary to the results for sodium hypochlorite reported in the article by Best et al, mentioned above, where an active chlorine level of 10,000 ppm was found necessary to pass the tuberculocidal activity test. Examples 5A-5E show the result of increasing the amount of bleaching compound, sodium dichloroisocyanate, present in a disinfectant composition that depends on the quaternary ammonium compounds for its disinfecting action. The level of quaternary ammonium compound remained essentially constant as more and more bleaching compound was added. A synergistic effect between the quaternary ammonium compounds and the bleaching compound was observed at active chlorine levels of 1 100 and 2200 ppm according to TB TEST 1, since it was found that these compositions were unexpectedly tuberculocidal. No tuberculocidal effect was observed for Examples 1A and 1B, even though much higher levels of the same quaternary ammonium compounds were present in the tested compositions. In the same manner, Examples 2A and 2B showed that sodium dichloroisocyanate itself was not tuberculocidal at the same active chlorine levels as found in examples 5D and 5E. Example 3 with more than twice the level of sodium dichloroisocyanate that Example 2B was found to be tuberculocidal in the absence of quaternary ammonium compounds. Comparative Examples 6A-6C demonstrate that low amounts of bleaching compound in another cleaning formulation does not possess tuberculocidal properties according to TB TEST 1.

Examples 7-12 These Examples provided comparative and application examples of compositions employing different amounts of quaternary ammonium compounds and bleaching compounds. Comparative Example 7A was prepared by mixing 100 ml of CONC. D 1: 128 with 1 00 ml of BLANQU EADOR DE ROPA 1: 20. Comparative Example 7B was prepared by mixing 1 00 ml of CONC. D 1: 128 with 100 ml of BLANQU EADOR DE ROPA 1: 35. Comparative Example 7C was prepared by mixing 100 ml of CONC. D 1: 128 with 100 ml of BLANQU EADOR DE ROPA 1: 45. Comparative Example 8A was prepared by mixing 100 ml of CONC D 1: 256 with 100 ml of CLOTHING WHITENER 1: 10. Comparative Example 8B was prepared by mixing 1 00 ml of CON CD 1: 256 with 100 ml of BLEACHER OF CLOTHING 1: 20.

Comparative Example 8C was prepared by mixing 100 ml of CONC. D 1: 256 with 100 ml of CLOTHING BLEACH 1: 35. Comparative Example 9A was prepared by first dissolving four SDI TABLETS in 1890 ml of sterile purified water. Then 100 ml of that solution was mixed with 100 ml of CONC. D 1: 128. Comparative Example 9B was prepared by first dissolving two SDI TABLETS in 1890 ml of sterile purified water. Then 100 ml of that solution was mixed with 100 ml of CONC. D 1: 128. Application example 10 was prepared by mixing 100 ml of CONC. E 1: 100 with 100 ml of BLANQU EADOR DE ROPA 1: 20. Comparative Example 1 1 was prepared by first dissolving four SDI TABLETS in 1890 ml of sterile purified water. Then 100 ml of that solution was mixed with 100 ml of CONC. F 1: 10. Comparative Example 12 was prepared by first dissolving four SDI TABLETS in 1890 ml of sterile purified water. Then 100 ml of that solution was mixed with 100 ml of CONC. G. The level of active chlorine in each of the resulting diluted disinfectant compositions was determined by the METHOD OF TITULACIÓN YODOMETR ICO within one hour of dilution. The pH of the compositions was also measured. These values are reported in the Table I I. Table I I also lists the total calculated levels of quaternary ammonium compounds for each diluted disinfectant composition that has them. The results of evaluating the disinfectant compositions diluted via TB TEST 4 are reported in Table I I.

Table 1 . Total amount of quaternary ammonium compound present.

Comparative Examples 7A-7C evaluated various levels of CLOTHING ERA WHEEL while maintaining the level of total quaternary ammonium compound at 660 ppm. None of these compositions passed TB TEST 2. Comparative Examples 8A-8B evaluated the use of much higher levels of CLOTHING EVAANE than in Examples 7A-7C, but half of the total quaternary ammonium compound level was used. No disinfectant composition passed TB TEST 2. Comparative Example 8C used approximately half the amount of the total quaternary ammonium compound and CLOTHING BLEACH as in Comparative Example 7A. Failed to pass TB TEST 2. Comparative Examples 9A and 9B were comparable to Examples 7A and 7C, respectively, except sodium dichloroisocyanate was used as the bleaching compound. Both failed TB TEST 2. Application Example 10 falls within the amount of quaternary ammonium compound level and bleach compound (sodium hypochlorite) levels of the present invention. TB TEST 2 passed and in this way it was considered to be a tuberculocidal disinfectant. Comparative Example 1 1 gave an irregular result, which was not consistent with data obtained for similar compositions such as Example 5D, but was not retested. The composition was estimated as passing TB TEST 2 until almost the end of the test when it was considered to have failed. This may have been due to inaccuracies in the test method or possible interference with disinfecting action by one of the other ingredients present in the cleaning composition. Comparative Example 12 used a relatively high amount of a total quaternary ammonium compound together with an amount of bleaching compound below that found to be useful in the present invention. This composition failed TB TEST 2.

Based on the preceding Examples, a relatively narrow range of l quaternary ammonium compound (approximately 600 to 800 ppm) and active chlorine level of bleaching compounds (approximately 1100 to 2500 ppm) gave synergistic compositions that were cleaning compositions and tuberculocidal disinfectants.

Claims (1)

1. REIVI NDICATIONS A method for disinfecting a surface containing the tuberculosis bacillus comprising applying to said surface an aqueous composition comprising, as disinfecting agents, an amount of bleaching compound containing effective chlorine to provide from about 1.1 to about 2.500 parts. per million by weight of available chlorine level and from about 600 to about 800 parts per million by weight of a bactericidal organic quaternary ammonium compound The method of claim 1, wherein the bleaching compound is selected from the group consisting of hypochlo- alkali metals, alkaline earth metal hypochloptes, hypochlocyte addition compounds, chloramines, clopmines, chloramides, clopmides and mixtures thereof, and the bactericidal quaternary ammonium compound is selected from the group consisting of d (C8-C? 2 alkyl) d? (C1-C4 alk) ammonium, salts of (C? 2-C? 8 alkyl?) Di (C1-C4 alkyl) u? l) benzylmonium, salts of (C12-C? 8 alk?) d? (C1-C4 alk?) et? lbenc? lamon? o, and mixtures thereof The method of the claim 2, wherein the bleaching compound is selected from the group consisting of sodium hypochlorite and sodium dichloroisocyanurate and the quaternary ammonium compound is selected from the group consisting of d? (C8-C? 2) d? Met? Lamon? O, halides of (C? 2-C18 alk?) D? Met? Lbenc? Lamon? Or halides of (C? 2-C? 8 alk? L) d? ethylbenzylammonium methanol, and mixtures thereof The method of claim 3, wherein the bleaching compound is selected from the group consisting of sodium hypochlo- pocy, potassium hypochlo- type, sodium dichloroisocyanurate and potassium dichloroisocyanurate, and the ammonium compound. Quaternary is selected from the group consisting of d? (C8-C12 alk?) d? met? lamon? o chlorides, (C? 2-C? 8 alk? l) d? met? lbenc? lamon chlorides? and mixtures thereof The method of claim 4, wherein the compound is selected from the group consisting of sodium hypochlocite and sodium dichloroisocyanurate, and the quaternary ammonium compound is selected from the group consisting of didecyldimethylammonium chloride, chlorides of (C? 2-C? 6 alk?) D? Met? Lbenc? Lamon? O and mixtures thereof The method of claim 5 wherein the composition contains an amount of effective compound. to provide from about 1, 100 to 2,200 parts per million of available chlorine level and from about 650 to 700 parts per million of a didecyldimethylammonium chloride mixture, and chlorides of (C 2 -C 16 alkole) d? The method of claim 1, wherein the bleaching compound is mixed with the quaternary ammonium compound just prior to the step of applying the composition to the surface. The method of claim 2, wherein the Bleaching compound is mixed with the quaternary ammonium compound just before the step of applying the composition to the surface. The method of claim 4, wherein the bleaching compound is mixed with the quaternary ammonium compound just before the step of applying the composition to the surface. The method of claim 6, wherein the bleaching compound is mixed with the compound. of quaternary ammonium just before the step of applying the composition to the surface A composition for disinfecting a surface containing the tuberculosis bacillus comprising an aqueous composition comprising, as disinfecting agents, an amount of an effective chlorine-containing bleach compound to provide from about 1, 100 to about 2,500 parts per million by weight of available chlorine level, and from about 600 to about 800 parts by million by weight of a bactericidal organic quaternary ammonium compound. The composition of claim 1, in where the bleaching compound is selected of the group consisting of alkali metal hypocloptes, alkaline earth metal hypochlorites, hypochlocyte addition compounds, chloramines, clopmines, chloramides, clopmides and mixtures thereof, and the bactericidal quaternary ammonium compound is selected from the group consisting of d? (C8-C? 2 alk salts. l) d) (C? -C4 alk) ammonium, salts of (C? 2-C? 8 alkyl) di (C? -C alkyl) benzylammon?, salts of (C? 2-C) ? 8 alkyl) d? (C? -C4 alk?) Ethylbenzamide, and mixtures thereof The composition of claim 12, wherein the bleaching compound is selected from the group consisting of sodium hypochlocite and dichloroisocyanurate of sodium and the quaternary ammonium compound is selected from the group consisting of d? (C8-C? 2 alk?) dimethylammonium halides, (C12-C? 8 alk?) d? met halides ? lbencilamon? o and mixtures thereof The composition of claim 13, wherein the bleaching compound is selected from the group consisting of sodium hypochlocite, potassium hypochlocite, sodium dichloroisocyanurate and dichloroisocyanurate of potassium, and the quaternary ammonium compound is selected from the group consisting of d? (C8-C? 2 alk?) d? met? lamon? o chlorides, (C? 2-C? 8 alkyl) d? Met? Lbenc? Lamon? O and mixtures thereof The composition of claim 14, wherein the compound is selected from the group consisting of sodium hypochlocite and sodium dichloroisocyanurate, and the quaternary ammonium compound is selected from the group consisting of didecyldimethylammonium chloride, chlorides of (C 2-C 16 alk) d? met? lbenc? lamon? o and mixtures thereof The composition of claim 15, wherein the composition contains an amount of effective compound to provide from about 1, 1 00 to 2,200 parts per million available chlorine level and from about 650 to 700 parts per million of a didecyldimethylammonium chloride mixture, and (C 12 -C 16 alkyl) dimethyl benzylammonium chlorides. A two-component composition for disinfecting a surface containing the tuberculosis bacillus comprising an aqueous composition comprising, as disinfecting agents, an amount of an effective chlorine-containing bleach compound to provide from about 1, 100 to about 2,500 parts per million by weight of available chlorine level and from about 600 to about 800 parts per million by weight of a bactericidal organic quaternary ammonium compound, wherein the bleaching compound is maintained as a first component separately from the second component comprising the ammonium compound Quaternary, where the two components are mixed just before application to the surface. The two component composition of claim 17, wherein the bleaching compound is selected from the group consisting of alkali metal hypochlorites, alkaline earth metal hypochlorites, hypochlorite addition compounds, chloramines, chlorimines, chloramides, chlorimides, and mixtures thereof , and the bactericidal quaternary ammonium compound is selected from the group consisting of di (C8-C? 2 alkyl) di (C? -C alkyl) ammonium salts, salts of (C? 2-C18 alkyl) di (C? -C alkyl) benzylammonium, salts of (C? 2-C? 8 alkyl) di (C? -C alkyl) ethylbenzylammonium, and mixtures thereof. 19. The composition of claim 18, wherein the bleaching compound is selected from the group consisting of sodium hypochlorite and sodium dichloroisocyanurate and the quaternary ammonium compound is selected from the group consisting of di (C8-C? 2 alkyl) halides. dimethylammonium, (C 2 -C 8 alkyl) dimethylbenzylammonium halides, (C 2 -C 8 alky) dimethyl ethylbenzylammonium halides and mixtures thereof. The composition of claim 19, wherein the bleaching compound is selected from the group consisting of sodium hypochlorite, potassium hypochlorite, sodium dichloroisocyanurate and potassium dichloroisocyanurate, and the quaternary ammonium compound is selected from the group consisting of chlorides of di (C8-C? 2 alkyl) dimethylammonium, chlorides of (C? 2-C1-8 alkyl) dimethylbenzylammonium and mixtures thereof. twenty-one . The composition of claim 20, wherein the compound is selected from the group consisting of sodium hypochlocite and sodium dichloroisocyanurate, and the quaternary ammonium compound is selected from the group consisting of didecyldimethylammonium chloride, (C12-C16 alkyl) chlorides dimethylbenzylammonium and mixtures thereof. The composition of claim 21, wherein the composition contains an effective amount of compound to provide from about 1, 100 to 2,200 parts per million level of available chlorine and from about 650 to 700 parts per million of a chloride mixture. of didecyldimethylammonium, and chlorides of (d2-C16 alkyl) dimethyl-benzylammonium.