WO2001037659A2 - Biocide compositions - Google Patents
Biocide compositions Download PDFInfo
- Publication number
- WO2001037659A2 WO2001037659A2 PCT/GB2000/004421 GB0004421W WO0137659A2 WO 2001037659 A2 WO2001037659 A2 WO 2001037659A2 GB 0004421 W GB0004421 W GB 0004421W WO 0137659 A2 WO0137659 A2 WO 0137659A2
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- biocide composition
- composition according
- opa
- biocide
- edta
- Prior art date
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/04—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aldehyde or keto groups, or thio analogues thereof, directly attached to an aromatic ring system, e.g. acetophenone; Derivatives thereof, e.g. acetals
Definitions
- the invention relates to biocide compositions, and more particularly, but not exclusively, to biocide compositions with a good cleansing action for use in removing and killing microbes in ail situations.
- Biocide compositions are well known. Some are based on single chemical components and others are formulations made by combining multiple chemicals. Since many biocides are chemically neutralized and thereby inactivated by organic matter, it is important to thoroughly clean the area to be disinfected before using those biocides. A good example of this is hypochlorous acid or bleach, a good biocide under clean conditions but it has the disadvantage of being less effective in practical situations. Those skilled in biocide technology know that many biocides are inactivated by detergents. This fact becomes critically important in the field of hand washes in hospitals when staff use one hand wash when they simply need to clean their hands or first arrive at work, and a different hand wash when they need to remove bacterial contamination. Anionic components are often used in the cleansing hand washes, and Chlorhexidine is commonly used as the biocidai component in the anti-microbial hand washes, but anionic chemicals inactivate chlorhexidine.
- EP 0836803 discloses an aqueous disinfecting composition comprising from 1 to 20% aldehydes, especially glutaraldehyde, 3 to 35% salts, especially quaternary ammonium chlorides, 1 to 15% ethoxylated fatty alcohol, and from 0.5 to 15% of an insecticidal agent selected from synthetic pyrethroids. Although this composition has the advantage of a cleaning component, the aldehyde component still produces toxic vapours and the alcohol has the disadvantage of being flammable.
- biocide composition with both cleaning and disinfecting capacity and which is safe for general and widespread use. It is a further object of this invention to provide a biocide composition which is usable in both the solid and liquid forms.
- the invention provides a biocide composition comprising at least one of each of the following: (I) a surtace-active chemical (ii) an aldehyde, and (iii) a complexing agent.
- a biocide composition comprising at least one non-volatile aldehyde.
- the invention provides for a biocide composition which is provided in a solid form and subsequently dissolved in a solvent including, but not exclusively, water to make a biocidai solution.
- the contaminated discs When required the contaminated discs were dipped into the test formulation for the required contact time of five minutes, removed and placed into 20 ml of Bacillus growth medium with the dilution ratio being used to terminate the anti-microbial activity. Control contaminated discs were dipped for five minutes in growth medium rather than disinfectant and subsequently treated in the same manner as the test discs. That growth medium containing the discs was incubated for an appropriate number of days, and then a sample was plated onto the appropriate agar and the plates incubated. In the following data, if one or more colonies grew on the agar the formulation was deemed to have failed as a sporicide. If no colonies were seen on the agar, but the control plates had heavy growth, the formulation was deemed to have passed as a sporicide.
- Formulation Ingredients Bacterial Polio Number Spores Virus
- Formulation 1 a commercially available disinfectant, failed to kill spores, but with the addition of 0.25% OPA it became sporicidal, whereas with 0.1% added OPA it did not. OPA alone in water at 0.5% failed to kill spores. Therefore the minimal sporicidal level of OPA is greater than 0.5% when tested alone and greater than 0.1% when used in a synergistic mixture with QAC's.
- Formulation 6 the commercially available product of Formulation 1 with 0.1% OPA and 0.05% EDTA added, was sporicidal.
- Formulations 7 to 10 followed a similar sequence but with a single QAC. The
- Formulations 11 to 14 followed the same sequence with a known product based on two QAC's tested against polio virus.
- Formulations 15 to 18 are based on 2% cetyltrimethyl ammonium bromide, a QAC that is a solid at ambient temperatures. Adding either 0.05% EDTA or 0.25% OPA alone failed to make it sporicidal, but adding 0.05% EDTA and 0.25% OPA together made it sporicidal.
- Formulation 18 is a biocide solution made by dissolving the solid biocide composition made up of solid QAC, EDTA and OPA in water.
- the best modes of carrying out the invention relate to the choice of QAC and the decision of whether one or more than one QAC should be used.
- the QAC group offers a wide variety of features - some are excellent conditioners of skin and fabrics, others have particularly good cleaning properties and others are solids at ambient temperatures which allows for powdered formulations that can additionally be formed into tablets.
- the QAC's and amine oxide are mixed at room temperature, the EDTA and OPA are dissolved into that mixture and a pH modifier is added slowly until the desired pH is achieved. The appropriate amount of water is then added, depending on whether a concentrate or the use dilution is required. Readers will understand that the formulations and components quoted here are by way of example only and do not represent the limit of the possible choices of surface active agent, compiexing agent or aldehyde, nor the concentrations to be used.
- OPA was selected as the example aldehyde because it is a solid thereby allowing for the powdered composition that was dissolved in solvent, in this case water, to provide formulation 18.
- the invention provides biocide compositions suitable for safe and widespread use in the cleaning, sanitising, disinfecting and sterilising fields in all industries and markets.
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- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
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- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
- Medicinal Preparation (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Presented is the means to generate biocide compositions by the combination of surface-active agents, complexing/chelating agents and aldehydes. Formulations based on quaternary ammonium compounds, ethylene diamine tetra-acetic acid and orthophthalaldehyde are shown by way of example. Such formulations have the advantage of being cost effective for widespread usage and safe enough for use in household, medical, institutional, agricultural and industrial fields without creating adverse effects on users, patients, equipment or the environment. One of the example formulations provides for a composition that can either be in powder or tablet format.
Description
BIOCIDE COMPOSITIONS
Technical Field The invention relates to biocide compositions, and more particularly, but not exclusively, to biocide compositions with a good cleansing action for use in removing and killing microbes in ail situations.
Background Art Biocide compositions are well known. Some are based on single chemical components and others are formulations made by combining multiple chemicals. Since many biocides are chemically neutralized and thereby inactivated by organic matter, it is important to thoroughly clean the area to be disinfected before using those biocides. A good example of this is hypochlorous acid or bleach, a good biocide under clean conditions but it has the disadvantage of being less effective in practical situations. Those skilled in biocide technology know that many biocides are inactivated by detergents. This fact becomes critically important in the field of hand washes in hospitals when staff use one hand wash when they simply need to clean their hands or first arrive at work, and a different hand wash when they need to remove bacterial contamination. Anionic components are often used in the cleansing hand washes, and Chlorhexidine is commonly used as the biocidai component in the anti-microbial hand washes, but anionic chemicals inactivate chlorhexidine.
High toxicity profiles make many currently used biocides unacceptable. Glutaraldehyde is widely used for the decontamination of flexible endoscopes, but the vapour it releases is believed to cause occupational asthma. EP 0836803 discloses an aqueous disinfecting composition comprising from 1 to 20% aldehydes, especially glutaraldehyde, 3 to 35% salts, especially quaternary
ammonium chlorides, 1 to 15% ethoxylated fatty alcohol, and from 0.5 to 15% of an insecticidal agent selected from synthetic pyrethroids. Although this composition has the advantage of a cleaning component, the aldehyde component still produces toxic vapours and the alcohol has the disadvantage of being flammable.
Disclosure of Invention
It is an object of this invention to provide a biocide composition with both cleaning and disinfecting capacity and which is safe for general and widespread use. It is a further object of this invention to provide a biocide composition which is usable in both the solid and liquid forms.
Accordingly, the invention provides a biocide composition comprising at least one of each of the following: (I) a surtace-active chemical (ii) an aldehyde, and (iii) a complexing agent. The invention also provides for a biocide composition comprising at least one non-volatile aldehyde.
In addition, the invention provides for a biocide composition which is provided in a solid form and subsequently dissolved in a solvent including, but not exclusively, water to make a biocidai solution.
Brief Description of the Table
Eighteen formulations are shown which collectively provide evidence of the poor effectiveness of prior art formulations against bacterial spores and Polio virus, compared to the effectiveness of formulations based on the invention. The Polio virus tests followed the protocol of Nagington and colleagues published in the British Journal of Ophthalmology, 1983, volume 67, page 674, and
were performed by a member of that team. The spore tests were based on the following protocol: filter-paper discs of several millimetres diameter were dipped into a suspension of Bacillus subtilis spores, air dried and stored. When required the contaminated discs were dipped into the test formulation for the required contact time of five minutes, removed and placed into 20 ml of Bacillus growth medium with the dilution ratio being used to terminate the anti-microbial activity. Control contaminated discs were dipped for five minutes in growth medium rather than disinfectant and subsequently treated in the same manner as the test discs. That growth medium containing the discs was incubated for an appropriate number of days, and then a sample was plated onto the appropriate agar and the plates incubated. In the following data, if one or more colonies grew on the agar the formulation was deemed to have failed as a sporicide. If no colonies were seen on the agar, but the control plates had heavy growth, the formulation was deemed to have passed as a sporicide.
Formulation Ingredients Bacterial Polio Number Spores Virus
1 1.8% Didecyldimethylethoxy ammonium chloride 0.7% Benzalkonium chloride
0.4% Cocopropylenediamine guanidinium acetate Fail
2 1 + 0.05% EDTA Fail
3 0.5% OPA in water Fail
4 Water alone Fail
5 1 + 0.1% OPA Fail
6 1 + 0.05% EDTA + 0.1 % OPA Pass
7 2% Alkyl dimethylbenzalkonium chloride Fail
8 7+ 0.05% EDTA Fail
9 7 + 0.25% OPA Fail
10 7 + 0.05% EDTA + 0.25% OPA Pass
11 0.05% Didecyldimethyl ammonium chloride
0.05% Alkyldimethylbenzyl ammonium chloride Fail
12 11 + 0.05% EDTA Fail
13 11 + 0.25% OPA Fail
14 11 + 0.05% EDTA + 0.25% OPA Pass
15 2% Cetyltrimethyl ammonium bromide Fail
16 15 + 0.05% EDTA Fail
17 15 + 0.25% OPA Fail
18 15 + 0.05% EDTA + 0.25% OPA Pass
These results clearly show the advances brought by the QAC, EDTA and aldehyde combination. Formulation 1, a commercially available disinfectant, failed to kill spores, but with the addition of 0.25% OPA it became sporicidal, whereas with 0.1% added OPA it did not. OPA alone in water at 0.5% failed to kill spores. Therefore the minimal sporicidal level of OPA is greater than 0.5% when tested alone and greater than 0.1% when used in a synergistic mixture with QAC's. Formulation 6, the commercially available product of Formulation 1 with 0.1% OPA and 0.05% EDTA added, was sporicidal. Formulations 7 to 10 followed a similar sequence but with a single QAC. The
QAC failed to kill spores when used on its own or when mixed with either 0.05% EDTA or 0.25% OPA, but the combination of all three was sporicidal.
Formulations 11 to 14 followed the same sequence with a known product based on two QAC's tested against polio virus. The combination of the two QAC's on its own failed to kill polio virus, and the addition of either 0.05% EDTA or 0.25% OPA failed to alter that result. But adding both 0.05% EDTA and 0.25% OPA to the two QAC's produced a formulation that killed polio virus.
Formulations 15 to 18 are based on 2% cetyltrimethyl ammonium bromide, a QAC that is a solid at ambient temperatures. Adding either 0.05% EDTA or 0.25%
OPA alone failed to make it sporicidal, but adding 0.05% EDTA and 0.25% OPA together made it sporicidal. Formulation 18 is a biocide solution made by dissolving the solid biocide composition made up of solid QAC, EDTA and OPA in water.
Best Modes of Carrying Out the Invention
The best modes of carrying out the invention relate to the choice of QAC and the decision of whether one or more than one QAC should be used. The QAC group offers a wide variety of features - some are excellent conditioners of skin and fabrics, others have particularly good cleaning properties and others are solids at ambient temperatures which allows for powdered formulations that can additionally be formed into tablets.
The example formulation below, which is based on this invention, will demonstrate the best mode of carrying out the invention.
INGREDIENT %WV
Benzalkonium chloride 0.01-5.0
Didecyldimethyethoxy ammonium chloride 0.01-5.0
Amine Oxide 0.01-5.0
Ethylene diamine Tetra Acetic acid 0.01-5.0
Orthophthalaldehyde 0.01-0.5
Water To 100%
The QAC's and amine oxide are mixed at room temperature, the EDTA and OPA are dissolved into that mixture and a pH modifier is added slowly until the desired pH is achieved. The appropriate amount of water is then added, depending on whether a concentrate or the use dilution is required.
Readers will understand that the formulations and components quoted here are by way of example only and do not represent the limit of the possible choices of surface active agent, compiexing agent or aldehyde, nor the concentrations to be used. OPA was selected as the example aldehyde because it is a solid thereby allowing for the powdered composition that was dissolved in solvent, in this case water, to provide formulation 18.
Industrial Application The invention provides biocide compositions suitable for safe and widespread use in the cleaning, sanitising, disinfecting and sterilising fields in all industries and markets.
Claims
1. A biocide composition comprising at least one of each of the following:
(i) a surface-active chemical; (ii) an aldehyde, and (iii) a compiexing agent.
2. A biocide composition according to claim 1 , wherein the surface-active chemical comprises a Quaternary Ammonium Compound (QAC).
3. A biocide composition according to claim 1 or claim 2, wherein the aldehyde is a non-volatile aldehyde.
4. A biocide composition according to claim 3, wherein the aldehyde comprises orthophthalaldehyde (OPA).
5. A biocide composition according to any preceding claim, wherein the compiexing agent comprises ethylene diamine tetra-acetic acid (EDTA).
6. A biocide composition according to any preceding claim, wherein the composition is provided in powder or tablet form.
7. A biocide composition according to any preceding claim, wherein the composition is dissolved in a solvent to provide a biocidai solution.
8. A biocide composition according to claim 7, wherein the solvent is water.
9. A biocide composition according to claim 8, wherein the aqueous biocidai solution comprises by weight:
(i) 0.01-5.0% Didecyldimethyl ammonium chloride and 0.01-5.0% Alkyldimethylbenzyl ammonium chloride; 0.01-5.0% Amine Oxide
(ii) 0.01-5.0% EDTA, and (iii) 0.01-5.0% OPA.
0. A biocide composition substantially as herein described with reference to and as described in the examples.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU15306/01A AU1530601A (en) | 1999-11-24 | 2000-11-22 | Biocide compositions |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB9927614.9A GB9927614D0 (en) | 1999-11-24 | 1999-11-24 | Sterliant formulation |
| GB9927614.9 | 1999-11-24 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO2001037659A2 true WO2001037659A2 (en) | 2001-05-31 |
| WO2001037659A3 WO2001037659A3 (en) | 2001-10-18 |
Family
ID=10864945
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/GB2000/004421 WO2001037659A2 (en) | 1999-11-24 | 2000-11-22 | Biocide compositions |
Country Status (3)
| Country | Link |
|---|---|
| AU (1) | AU1530601A (en) |
| GB (1) | GB9927614D0 (en) |
| WO (1) | WO2001037659A2 (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009100879A2 (en) * | 2008-02-12 | 2009-08-20 | Lonza Inc. | Broad spectrum disinfecting and sterilizing composition |
| US20110009493A1 (en) * | 2008-02-12 | 2011-01-13 | Larry Kent Hall | Broad Spectrum Disinfecting and Sterilizing Composition |
| US20130171264A1 (en) * | 2010-06-21 | 2013-07-04 | Mtp Innovations | Disinfectant Composition |
| US8911665B2 (en) * | 2001-12-05 | 2014-12-16 | Aseptica, Inc. | Antiseptic compositions, methods and systems |
| CN112931505A (en) * | 2021-01-22 | 2021-06-11 | 海韵一剑大卫生科技有限公司 | Phthalic dicarboxaldehyde disinfectant, preparation method and application thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4661523A (en) * | 1984-10-29 | 1987-04-28 | Henkel Kommanditgesellschaft Auf Aktien | Disinfectant solutions having improved corrosion properties |
| US5344838A (en) * | 1985-01-18 | 1994-09-06 | Cetylite Industries, Inc. | Sterilant composition |
| FR2718923A1 (en) * | 1994-04-25 | 1995-10-27 | Sogeval | Aq. disinfectant soln. contg. ortho-phthalaldehyde |
| FR2743982A1 (en) * | 1996-01-30 | 1997-08-01 | Salkin Andre | Combination of aromatic aldehyde and quat. ammonium derivs. as antimicrobial agent |
| US5891922A (en) * | 1997-08-20 | 1999-04-06 | Preserve International, Preserve, Inc. | Cleaning and disinfectant biocide composition, and method for cleaning animal husbandry surfaces |
-
1999
- 1999-11-24 GB GBGB9927614.9A patent/GB9927614D0/en not_active Ceased
-
2000
- 2000-11-22 AU AU15306/01A patent/AU1530601A/en not_active Abandoned
- 2000-11-22 WO PCT/GB2000/004421 patent/WO2001037659A2/en active Application Filing
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4661523A (en) * | 1984-10-29 | 1987-04-28 | Henkel Kommanditgesellschaft Auf Aktien | Disinfectant solutions having improved corrosion properties |
| US5344838A (en) * | 1985-01-18 | 1994-09-06 | Cetylite Industries, Inc. | Sterilant composition |
| FR2718923A1 (en) * | 1994-04-25 | 1995-10-27 | Sogeval | Aq. disinfectant soln. contg. ortho-phthalaldehyde |
| FR2743982A1 (en) * | 1996-01-30 | 1997-08-01 | Salkin Andre | Combination of aromatic aldehyde and quat. ammonium derivs. as antimicrobial agent |
| US5891922A (en) * | 1997-08-20 | 1999-04-06 | Preserve International, Preserve, Inc. | Cleaning and disinfectant biocide composition, and method for cleaning animal husbandry surfaces |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8911665B2 (en) * | 2001-12-05 | 2014-12-16 | Aseptica, Inc. | Antiseptic compositions, methods and systems |
| WO2009100879A2 (en) * | 2008-02-12 | 2009-08-20 | Lonza Inc. | Broad spectrum disinfecting and sterilizing composition |
| WO2009100879A3 (en) * | 2008-02-12 | 2010-06-24 | Lonza Inc. | Broad spectrum disinfecting and sterilizing composition |
| US20110009493A1 (en) * | 2008-02-12 | 2011-01-13 | Larry Kent Hall | Broad Spectrum Disinfecting and Sterilizing Composition |
| US20130171264A1 (en) * | 2010-06-21 | 2013-07-04 | Mtp Innovations | Disinfectant Composition |
| US10827752B2 (en) * | 2010-06-21 | 2020-11-10 | Peter Finan | Disinfectant composition |
| CN112931505A (en) * | 2021-01-22 | 2021-06-11 | 海韵一剑大卫生科技有限公司 | Phthalic dicarboxaldehyde disinfectant, preparation method and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| AU1530601A (en) | 2001-06-04 |
| GB9927614D0 (en) | 2000-01-19 |
| WO2001037659A3 (en) | 2001-10-18 |
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