MX368177B - Proceso continuo de múltiples etapas para purificar anticuerpos. - Google Patents

Proceso continuo de múltiples etapas para purificar anticuerpos.

Info

Publication number
MX368177B
MX368177B MX2015015482A MX2015015482A MX368177B MX 368177 B MX368177 B MX 368177B MX 2015015482 A MX2015015482 A MX 2015015482A MX 2015015482 A MX2015015482 A MX 2015015482A MX 368177 B MX368177 B MX 368177B
Authority
MX
Mexico
Prior art keywords
multistep process
purifying antibodies
continuous multistep
continuous
antibodies
Prior art date
Application number
MX2015015482A
Other languages
English (en)
Other versions
MX2015015482A (es
Inventor
Duthe Didier
Landric-Burtin Laure
Hemet Céline
Mothes Benoît
Original Assignee
Sanofi Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sanofi Sa filed Critical Sanofi Sa
Publication of MX2015015482A publication Critical patent/MX2015015482A/es
Publication of MX368177B publication Critical patent/MX368177B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/12—Purification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Water Supply & Treatment (AREA)
  • Oncology (AREA)
  • Cell Biology (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)

Abstract

La presente invención se refiere a un proceso cromatográfico de tres etapas para la purificación de proteínas a pequeña y gran escala, específicamente anticuerpos monoclonales utilizando solo cuatro soluciones amortiguadoras hechas de una solución madre.
MX2015015482A 2013-05-06 2014-05-06 Proceso continuo de múltiples etapas para purificar anticuerpos. MX368177B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP13305593 2013-05-06
PCT/EP2014/059246 WO2014180852A1 (en) 2013-05-06 2014-05-06 Continuous multistep process for purifying antibodies

Publications (2)

Publication Number Publication Date
MX2015015482A MX2015015482A (es) 2016-03-21
MX368177B true MX368177B (es) 2019-09-23

Family

ID=48430636

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2015015482A MX368177B (es) 2013-05-06 2014-05-06 Proceso continuo de múltiples etapas para purificar anticuerpos.

Country Status (19)

Country Link
US (1) US10793622B2 (es)
EP (2) EP2994485B1 (es)
JP (2) JP6463734B2 (es)
KR (1) KR102272851B1 (es)
CN (2) CN116003579A (es)
AU (1) AU2014264697B2 (es)
BR (1) BR112015027812B1 (es)
CA (1) CA2911462C (es)
CY (1) CY1125281T1 (es)
DK (2) DK3301115T3 (es)
ES (2) ES2665645T3 (es)
HU (2) HUE038741T2 (es)
IL (1) IL242408B (es)
MX (1) MX368177B (es)
NO (1) NO2994485T3 (es)
RU (1) RU2662668C2 (es)
SG (1) SG11201508968XA (es)
TW (2) TWI631132B (es)
WO (1) WO2014180852A1 (es)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9458450B2 (en) 2012-03-15 2016-10-04 Flodesign Sonics, Inc. Acoustophoretic separation technology using multi-dimensional standing waves
US9950282B2 (en) 2012-03-15 2018-04-24 Flodesign Sonics, Inc. Electronic configuration and control for acoustic standing wave generation
US10967298B2 (en) 2012-03-15 2021-04-06 Flodesign Sonics, Inc. Driver and control for variable impedence load
US10704021B2 (en) 2012-03-15 2020-07-07 Flodesign Sonics, Inc. Acoustic perfusion devices
US20150275195A1 (en) * 2012-10-24 2015-10-01 Genzyme Corporation Elution of biomolecules from multi-modal resins using mes and mops as mobile phase modifiers
CN105555795A (zh) * 2013-09-17 2016-05-04 株式会社钟化 新抗体纯化方法和由该方法得到的抗体、以及使用了阳离子交换基团的新抗体纯化法和由该方法得到的抗体
US9725710B2 (en) 2014-01-08 2017-08-08 Flodesign Sonics, Inc. Acoustophoresis device with dual acoustophoretic chamber
GB201503578D0 (en) * 2015-03-03 2015-04-15 Ge Healthcare Bio Sciences Ab Sanitization method for affinity chromatography matrices
US11420136B2 (en) 2016-10-19 2022-08-23 Flodesign Sonics, Inc. Affinity cell extraction by acoustics
US11021699B2 (en) 2015-04-29 2021-06-01 FioDesign Sonics, Inc. Separation using angled acoustic waves
US11708572B2 (en) 2015-04-29 2023-07-25 Flodesign Sonics, Inc. Acoustic cell separation techniques and processes
US11377651B2 (en) 2016-10-19 2022-07-05 Flodesign Sonics, Inc. Cell therapy processes utilizing acoustophoresis
EP3015542A1 (de) * 2015-05-07 2016-05-04 Bayer Technology Services GmbH Modulare anlage und verfahren zur kontinuierlichen, keimreduzierten produktion und/oder aufbereitung eines produktes
US11474085B2 (en) 2015-07-28 2022-10-18 Flodesign Sonics, Inc. Expanded bed affinity selection
US11459540B2 (en) 2015-07-28 2022-10-04 Flodesign Sonics, Inc. Expanded bed affinity selection
US20170101437A1 (en) * 2015-10-09 2017-04-13 Hetero Drugs Ltd. Process for purification of darbepoetin alfa
US11214789B2 (en) 2016-05-03 2022-01-04 Flodesign Sonics, Inc. Concentration and washing of particles with acoustics
US11085035B2 (en) 2016-05-03 2021-08-10 Flodesign Sonics, Inc. Therapeutic cell washing, concentration, and separation utilizing acoustophoresis
US20190211055A1 (en) * 2016-09-14 2019-07-11 Kinbio Ltd. Methods of purifying and qualifying antibodies
EP3601332A1 (en) * 2017-03-24 2020-02-05 Council of Scientific and Industrial Research A process for the purification of recombinant antibody fragments
GB201711481D0 (en) * 2017-07-17 2017-08-30 Ucb Biopharma Sprl Protein purification
WO2019045149A1 (en) * 2017-08-31 2019-03-07 Green Cross Corporation PROCESS FOR PURIFYING SULFATASE PROTEIN
CA3085784A1 (en) 2017-12-14 2019-06-20 Flodesign Sonics, Inc. Acoustic transducer driver and controller
EP3769083A1 (en) 2018-03-21 2021-01-27 Waters Technologies Corporation Non-antibody high-affinity-based sample preparation, sorbents, devices and methods
EP3546475A1 (en) * 2018-03-27 2019-10-02 Sanofi Full flow-through process for purifying recombinant proteins
US20190367858A1 (en) * 2018-06-01 2019-12-05 Lonza Ltd. Midscale Model For Organic Growth and Phasing
KR102337683B1 (ko) * 2018-09-21 2021-12-13 주식회사 녹십자 고효율 항-tfpi 항체 조성물
EP3636657A1 (en) 2018-10-08 2020-04-15 Ablynx N.V. Chromatography-free antibody purification method
WO2020084503A1 (en) * 2018-10-26 2020-04-30 Cadila Healthcare Limited A composition comprising antibody with reduced level of basic variants thereof
WO2020183332A1 (en) * 2019-03-11 2020-09-17 Intas Pharmaceuticals Ltd. Purification of adalimumab using tandem chromatography
WO2020205928A1 (en) * 2019-04-03 2020-10-08 Genzyme Corporation Continuous production of recombinant proteins
WO2020258704A1 (zh) * 2019-06-28 2020-12-30 信达生物制药(苏州)有限公司 无缝连续流层析方法
KR102154952B1 (ko) * 2020-02-21 2020-09-10 프레스티지바이오로직스 주식회사 아달리무맙의 Non-Protein A 정제방법
CN112574321B (zh) * 2020-12-30 2023-10-20 上海赛金生物医药有限公司 一种捕获单克隆抗体-肿瘤坏死因子融合蛋白的亲和纯化方法
WO2022234412A1 (en) 2021-05-03 2022-11-10 Lupin Limited A process for purification of fc-fusion proteins

Family Cites Families (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989003840A1 (en) 1987-10-23 1989-05-05 Schering Corporation Method of purifying protein
UA35599C2 (uk) * 1993-09-21 2001-04-16 Хемосол Інк Спосіб очищення гемоглобіну з сирого розчину
CA2106612C (en) 1993-09-21 2001-02-06 Diana Pliura Displacement chromatography process
US5891741A (en) 1997-05-16 1999-04-06 Coulter International Corp. Antibody-aminodextran-phycobiliprotein conjugates
CN1136317C (zh) 1999-03-12 2004-01-28 中国人民解放军第四军医大学 单克隆抗体双段和单段的制备方法
US20020009445A1 (en) 2000-07-12 2002-01-24 Yansheng Du Human beta-amyloid antibody and use thereof for treatment of alzheimer's disease
US6602855B2 (en) 2001-04-30 2003-08-05 Syn X Pharma, Inc. Biopolymer marker indicative of disease state having a molecular weight of 1449 daltons
CN1504482A (zh) 2002-12-03 2004-06-16 北京优力凯生物技术有限责任公司 无细胞百日咳抗原蛋白的分离纯化方法
EP2439213B1 (en) 2004-04-21 2018-12-12 The Brigham and Women's Hospital, Inc. Poly-N-acetyl glucosamine (PNAG/DPNAG)-binding antibodies and methods of use thereof
RU2389552C2 (ru) * 2004-10-21 2010-05-20 Джи-И Хелткер Байо-Сайенсиз АБ Способ очистки антител
IL296666A (en) 2005-03-23 2022-11-01 Genmab As Antibodies against 38cd for the treatment of multiple myeloma
CN1814775A (zh) 2005-12-06 2006-08-09 中国海洋大学 一种抗病毒活性寡聚肽的制备方法
EP1914242A1 (en) * 2006-10-19 2008-04-23 Sanofi-Aventis Novel anti-CD38 antibodies for the treatment of cancer
CA2692814A1 (en) * 2007-07-11 2009-01-15 Novo Nordisk A/S Purification of factor viii using a mixed-mode or multimodal resin
CA2705582A1 (en) 2007-11-16 2009-05-22 The Rockefeller University Antibodies specific for the protofibril form of beta-amyloid protein
TWI472339B (zh) * 2008-01-30 2015-02-11 Genentech Inc 包含結合至ｈｅｒ２結構域ii之抗體及其酸性變異體的組合物
MX2010009398A (es) 2008-02-29 2010-11-12 Biogen Idec Inc Proteinas de fusion de inmunoglobulinas purificadas y los metodos para su purificacion.
JP2011523408A (ja) * 2008-05-15 2011-08-11 ノボ・ノルデイスク・エー／エス 抗体精製方法
CA2730018A1 (en) * 2008-07-18 2010-01-21 Talecris Biotherapeutics, Inc. Method of preparing alpha-1 proteinase inhibitor
EP2360183B1 (en) 2008-12-19 2016-07-27 Takeda Pharmaceutical Company Limited Antibody purification method by mixed mode chromatography utilizing an arginine-containing loading solution
CN102272146A (zh) 2009-01-13 2011-12-07 通用电气健康护理生物科学股份公司 用带负电的聚合物沉淀生物分子
KR101224468B1 (ko) * 2009-05-20 2013-01-23 주식회사 파멥신 신규한 형태의 이중표적항체 및 그 용도
SG177577A1 (en) * 2009-08-07 2012-03-29 Emd Millipore Corp Methods for purifying a target protein from one or more impurities in a sample
US9527010B2 (en) * 2009-09-25 2016-12-27 Ge Healthcare Bio-Sciences Corp. Separation system and method
WO2011049798A1 (en) 2009-10-20 2011-04-28 Merck Sharp & Dohme Corp. Use of mixed mode chromatography for the capture and purification of basic antibody products
US20130178608A1 (en) * 2009-12-29 2013-07-11 Samir Kulkarni Protein purification by ion exchange
ES2534936T3 (es) 2010-08-02 2015-04-30 Ratiopharm Gmbh Método para producir y purificar una sialiltransferasa soluble activa
US20120238730A1 (en) * 2011-03-15 2012-09-20 Abbott Laboratories Integrated approach to the isolation and purification of antibodies
WO2012135415A1 (en) * 2011-03-29 2012-10-04 Glaxosmithkline Llc Buffer system for protein purification
ES2915263T3 (es) 2011-06-17 2022-06-21 Roche Diagnostics Hematology Inc Solución y procedimiento para el histoprocesamiento de muestras biológicas
US9096648B2 (en) * 2011-08-19 2015-08-04 Emd Millipore Corporation Methods of reducing level of one or more impurities in a sample during protein purification
EP2578286A1 (en) * 2011-10-04 2013-04-10 Merck Patent GmbH Method and apparatus for chromatographic purification
AU2012328739B2 (en) * 2011-10-25 2017-11-30 Prothena Biosciences Limited Antibody formulations and methods
WO2013075740A1 (en) 2011-11-23 2013-05-30 Sanofi Antibody purification method

Also Published As

Publication number Publication date
EP3301115B1 (en) 2021-11-10
US20160083454A1 (en) 2016-03-24
EP3301115A1 (en) 2018-04-04
RU2015151869A (ru) 2017-06-13
TWI631132B (zh) 2018-08-01
MX2015015482A (es) 2016-03-21
EP2994485B1 (en) 2018-01-10
EP2994485A1 (en) 2016-03-16
DK3301115T3 (da) 2022-01-31
KR102272851B1 (ko) 2021-07-02
TW201908331A (zh) 2019-03-01
KR20160005047A (ko) 2016-01-13
IL242408B (en) 2020-04-30
BR112015027812B1 (pt) 2021-03-30
JP2016519137A (ja) 2016-06-30
JP6463734B2 (ja) 2019-02-06
BR112015027812A2 (pt) 2017-07-25
WO2014180852A1 (en) 2014-11-13
CY1125281T1 (el) 2023-06-09
HUE057119T2 (hu) 2022-04-28
AU2014264697B2 (en) 2019-08-22
RU2662668C2 (ru) 2018-07-26
DK2994485T3 (en) 2018-04-23
HUE038741T2 (hu) 2018-11-28
AU2014264697A1 (en) 2015-11-26
JP2019104735A (ja) 2019-06-27
CA2911462A1 (en) 2014-11-13
SG11201508968XA (en) 2015-11-27
TW201522363A (zh) 2015-06-16
CN116003579A (zh) 2023-04-25
CA2911462C (en) 2021-04-06
ES2665645T3 (es) 2018-04-26
TWI675041B (zh) 2019-10-21
NO2994485T3 (es) 2018-06-09
ES2905675T3 (es) 2022-04-11
CN105358572A (zh) 2016-02-24
US10793622B2 (en) 2020-10-06

Similar Documents

Publication Publication Date Title
MX368177B (es) Proceso continuo de múltiples etapas para purificar anticuerpos.
MX364908B (es) Purificación de proteínas usando amortiguador bis-tris.
HK1217344A1 (zh) 單克隆抗體的純化工序
FR3025515B1 (fr) Procede de purification d'un anticorps monoclonal
MX368142B (es) Metodo de clonacion, expresion y purificacion novedoso para la preparacion de ranibizumab.
EA201600429A1 (ru) Способ очистки 2,5-дихлорфенола
EP2987801A4 (en) PROCESS FOR PURIFYING ANTIBODY PROTEIN
HUE049661T2 (hu) Eljárás biomassza hidrolizátum tisztítására
EP3291837A4 (en) MONOCLONAL ANTIBODY COCOA TAILS FOR THE TREATMENT OF EBOLA INFECTIONS
EA201700217A1 (ru) Способ очистки для выделения и коммерческого производства рекомбинантной tnk-tpa (тенектеплазы)
EP3092002A4 (en) Method of purifying monoclonal antibodies
EP3110790A4 (en) Methods and systems for purifying an acetonitrile waste stream and methods for synthesizing oligonucleotides using purified acetonitrile waste streams
MX2014013754A (es) Metodos de cristalizacion para la purificacion de anticuerpos monoclonales.
MX2015010006A (es) Purificacion de proteinas mejorada a traves de una etapa de desglicosilacion.
IL289959A (en) Antibody purification methods
IL277056A (en) Methods for purifying antibodies
GB201911685D0 (en) Process for purifying monoclonal antibodies
MX2019006783A (es) Procedimiento para la preparacion de 4-amino-1-( (1s, 4r, 5s) -2-fluoro-4,5-dihidroxi-3-hidroximetil-ciclopent-2-enil)-1h-pirim idin-2-ona.
MX2019011343A (es) Un procedimiento para la purificacion de fragmentos de anticuerpos recombinantes.
ZA201604024B (en) Improved feeding strategies and purification processes for monoclonal antibody production
IL276816A (en) Antibody purification
BR112016012664A2 (pt) Processo de purificação de 2,5-diclorofenol
RU2012143225A (ru) Способ выделения и очистки иммобилизованного фермента
TH1501001171A (th) กระบวนการสำหรับการแยกก๊าซกรดออกจากกระแสของไหลที่ประกอบด้วยน้ำ

Legal Events

Date Code Title Description
FG Grant or registration