SG11201508968XA - Continuous multistep process for purifying antibodies - Google Patents

Continuous multistep process for purifying antibodies

Info

Publication number
SG11201508968XA
SG11201508968XA SG11201508968XA SG11201508968XA SG11201508968XA SG 11201508968X A SG11201508968X A SG 11201508968XA SG 11201508968X A SG11201508968X A SG 11201508968XA SG 11201508968X A SG11201508968X A SG 11201508968XA SG 11201508968X A SG11201508968X A SG 11201508968XA
Authority
SG
Singapore
Prior art keywords
multistep process
purifying antibodies
continuous multistep
continuous
antibodies
Prior art date
Application number
SG11201508968XA
Inventor
Didier Duthe
Céline Hemet
Laure Landric-Burtin
Benoît Mothes
Original Assignee
Sanofi Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sanofi Sa filed Critical Sanofi Sa
Publication of SG11201508968XA publication Critical patent/SG11201508968XA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/12—Purification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Sustainable Development (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Water Supply & Treatment (AREA)
  • Oncology (AREA)
  • Cell Biology (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
SG11201508968XA 2013-05-06 2014-05-06 Continuous multistep process for purifying antibodies SG11201508968XA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP13305593 2013-05-06
PCT/EP2014/059246 WO2014180852A1 (en) 2013-05-06 2014-05-06 Continuous multistep process for purifying antibodies

Publications (1)

Publication Number Publication Date
SG11201508968XA true SG11201508968XA (en) 2015-11-27

Family

ID=48430636

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201508968XA SG11201508968XA (en) 2013-05-06 2014-05-06 Continuous multistep process for purifying antibodies

Country Status (19)

Country Link
US (1) US10793622B2 (en)
EP (2) EP3301115B1 (en)
JP (2) JP6463734B2 (en)
KR (1) KR102272851B1 (en)
CN (2) CN116003579A (en)
AU (1) AU2014264697B2 (en)
BR (1) BR112015027812B1 (en)
CA (1) CA2911462C (en)
CY (1) CY1125281T1 (en)
DK (2) DK3301115T3 (en)
ES (2) ES2665645T3 (en)
HU (2) HUE057119T2 (en)
IL (1) IL242408B (en)
MX (1) MX368177B (en)
NO (1) NO2994485T3 (en)
RU (1) RU2662668C2 (en)
SG (1) SG11201508968XA (en)
TW (2) TWI675041B (en)
WO (1) WO2014180852A1 (en)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9950282B2 (en) 2012-03-15 2018-04-24 Flodesign Sonics, Inc. Electronic configuration and control for acoustic standing wave generation
US10704021B2 (en) 2012-03-15 2020-07-07 Flodesign Sonics, Inc. Acoustic perfusion devices
US9458450B2 (en) 2012-03-15 2016-10-04 Flodesign Sonics, Inc. Acoustophoretic separation technology using multi-dimensional standing waves
US10967298B2 (en) 2012-03-15 2021-04-06 Flodesign Sonics, Inc. Driver and control for variable impedence load
CN105777862A (en) * 2012-10-24 2016-07-20 建新公司 Elution of biomolecules from multi-modal resins using MES and MOPS as mobile phase modifiers
EP3048109A4 (en) * 2013-09-17 2017-04-19 Kaneka Corporation Novel antibody purification method and antibody obtained therefrom, and novel antibody purification method using cation exchanger and antibody obtained therefrom
US9725710B2 (en) 2014-01-08 2017-08-08 Flodesign Sonics, Inc. Acoustophoresis device with dual acoustophoretic chamber
GB201503578D0 (en) * 2015-03-03 2015-04-15 Ge Healthcare Bio Sciences Ab Sanitization method for affinity chromatography matrices
US11708572B2 (en) 2015-04-29 2023-07-25 Flodesign Sonics, Inc. Acoustic cell separation techniques and processes
US11021699B2 (en) 2015-04-29 2021-06-01 FioDesign Sonics, Inc. Separation using angled acoustic waves
US11377651B2 (en) 2016-10-19 2022-07-05 Flodesign Sonics, Inc. Cell therapy processes utilizing acoustophoresis
US11420136B2 (en) 2016-10-19 2022-08-23 Flodesign Sonics, Inc. Affinity cell extraction by acoustics
EP3015542A1 (en) * 2015-05-07 2016-05-04 Bayer Technology Services GmbH Modular system and method for continuous, germ reduced production and/or processing of a product
US11474085B2 (en) 2015-07-28 2022-10-18 Flodesign Sonics, Inc. Expanded bed affinity selection
US11459540B2 (en) 2015-07-28 2022-10-04 Flodesign Sonics, Inc. Expanded bed affinity selection
US20170101437A1 (en) * 2015-10-09 2017-04-13 Hetero Drugs Ltd. Process for purification of darbepoetin alfa
US11214789B2 (en) 2016-05-03 2022-01-04 Flodesign Sonics, Inc. Concentration and washing of particles with acoustics
US11085035B2 (en) 2016-05-03 2021-08-10 Flodesign Sonics, Inc. Therapeutic cell washing, concentration, and separation utilizing acoustophoresis
US20190211055A1 (en) * 2016-09-14 2019-07-11 Kinbio Ltd. Methods of purifying and qualifying antibodies
CN110582510B (en) * 2017-03-24 2023-09-15 科学与工业研究委员会 Method for purifying recombinant antibody fragments
GB201711481D0 (en) * 2017-07-17 2017-08-30 Ucb Biopharma Sprl Protein purification
WO2019045149A1 (en) * 2017-08-31 2019-03-07 Green Cross Corporation Method for purifying a sulfatase protein
CN114900773A (en) 2017-12-14 2022-08-12 弗洛设计声能学公司 Acoustophoretic system, method for operating acoustophoretic system, and method for controlling acoustic transducer and acoustic system
US11155575B2 (en) 2018-03-21 2021-10-26 Waters Technologies Corporation Non-antibody high-affinity-based sample preparation, sorbent, devices and methods
EP3546475A1 (en) * 2018-03-27 2019-10-02 Sanofi Full flow-through process for purifying recombinant proteins
US20190367858A1 (en) * 2018-06-01 2019-12-05 Lonza Ltd. Midscale Model For Organic Growth and Phasing
KR102337683B1 (en) * 2018-09-21 2021-12-13 주식회사 녹십자 Highly efficient anti-TFPI antibody composition
EP3636657A1 (en) * 2018-10-08 2020-04-15 Ablynx N.V. Chromatography-free antibody purification method
WO2020084503A1 (en) * 2018-10-26 2020-04-30 Cadila Healthcare Limited A composition comprising antibody with reduced level of basic variants thereof
WO2020183332A1 (en) * 2019-03-11 2020-09-17 Intas Pharmaceuticals Ltd. Purification of adalimumab using tandem chromatography
SG11202110909XA (en) * 2019-04-03 2021-10-28 Genzyme Corp Continuous production of recombinant proteins
CN114026108A (en) * 2019-06-28 2022-02-08 信达生物制药(苏州)有限公司 Seamless continuous flow chromatography method
KR102154952B1 (en) * 2020-02-21 2020-09-10 프레스티지바이오로직스 주식회사 Non-Protein A Purification of Adalimumab
CN112574321B (en) * 2020-12-30 2023-10-20 上海赛金生物医药有限公司 Affinity purification method for capturing monoclonal antibody-tumor necrosis factor fusion protein
WO2022234412A1 (en) 2021-05-03 2022-11-10 Lupin Limited A process for purification of fc-fusion proteins

Family Cites Families (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0391934A1 (en) 1987-10-23 1990-10-17 Schering Corporation Method of purifying protein
CA2106612C (en) 1993-09-21 2001-02-06 Diana Pliura Displacement chromatography process
HU218159B (en) * 1993-09-21 2000-06-28 Hemosol Inc. Displacement chromatography process for production of purified hemoglobin product
US5891741A (en) 1997-05-16 1999-04-06 Coulter International Corp. Antibody-aminodextran-phycobiliprotein conjugates
CN1136317C (en) 1999-03-12 2004-01-28 中国人民解放军第四军医大学 Process for preparing bisegmentum and monosegmentum of monoclone antibody
US20020009445A1 (en) 2000-07-12 2002-01-24 Yansheng Du Human beta-amyloid antibody and use thereof for treatment of alzheimer's disease
US6602855B2 (en) 2001-04-30 2003-08-05 Syn X Pharma, Inc. Biopolymer marker indicative of disease state having a molecular weight of 1449 daltons
CN1504482A (en) * 2002-12-03 2004-06-16 北京优力凯生物技术有限责任公司 Separation and purification process for acellular whooping cough antigen albumen
DK1745075T3 (en) 2004-04-21 2013-06-24 Brigham & Womens Hospital POLY-N-ACETYLGLUCOSAMINE (PNAG / DPNAG) BINDING PEPTIDES AND PROCEDURES FOR USING IT
CA2583578C (en) 2004-10-21 2014-04-01 Ge Healthcare Bio-Sciences Ab Chromatography ligand
EP2535355B1 (en) 2005-03-23 2019-01-02 Genmab A/S Antibodies against CD38 for treatment of multiple myeloma
CN1814775A (en) 2005-12-06 2006-08-09 中国海洋大学 Method for preparing antiviral active oligomeric peptide
EP1914242A1 (en) * 2006-10-19 2008-04-23 Sanofi-Aventis Novel anti-CD38 antibodies for the treatment of cancer
RU2493163C2 (en) * 2007-07-11 2013-09-20 Ново Нордиск А/С Method of purifying coagulation factor viii protein and method of stabilising factor viii protein
EA201000809A1 (en) 2007-11-16 2010-12-30 Те Рокфеллер Юниверсити ANTIBODIES SPECIFIC WITH RESPECT TO THE PROTOFIBRILLARY FORM OF β-AMYLOID PROTEIN
TWI472339B (en) * 2008-01-30 2015-02-11 Genentech Inc Composition comprising antibody that binds to domain ii of her2 and acidic variants thereof
MX2010009398A (en) * 2008-02-29 2010-11-12 Biogen Idec Inc Purified immunoglobulin fusion proteins and methods of their purification.
CN103396480A (en) 2008-05-15 2013-11-20 诺沃—诺迪斯克有限公司 Antibody purification process
NZ590257A (en) * 2008-07-18 2012-08-31 Grifols Therapeutics Inc Method of preparing alpha-1 proteinase inhibitor
EP2360183B1 (en) 2008-12-19 2016-07-27 Takeda Pharmaceutical Company Limited Antibody purification method by mixed mode chromatography utilizing an arginine-containing loading solution
JP2012515161A (en) 2009-01-13 2012-07-05 ジーイー・ヘルスケア・バイオサイエンス・アクチボラグ Biomolecule precipitation by negatively charged polymers
KR101224468B1 (en) * 2009-05-20 2013-01-23 주식회사 파멥신 Bispecific antibody having a novel form and use thereof
IN2012DN00338A (en) * 2009-08-07 2015-05-22 Millipore Corp
US9527010B2 (en) * 2009-09-25 2016-12-27 Ge Healthcare Bio-Sciences Corp. Separation system and method
US20120208986A1 (en) 2009-10-20 2012-08-16 Wenger Marc D Use of mixed mode chromatography for the capture and purification of basic antibody products
EP2519536A4 (en) * 2009-12-29 2013-06-05 Reddys Lab Ltd Dr Protein purification by ion exchange
ES2534936T3 (en) 2010-08-02 2015-04-30 Ratiopharm Gmbh Method for producing and purifying an active soluble sialyltransferase
US20120238730A1 (en) * 2011-03-15 2012-09-20 Abbott Laboratories Integrated approach to the isolation and purification of antibodies
CA2831787A1 (en) 2011-03-29 2012-10-04 Glaxosmithkline Llc Buffer system for protein purification
EP2721391B1 (en) 2011-06-17 2022-04-06 Roche Diagnostics Hematology, Inc. Solution and method for histoprocessing of biological samples
US9096648B2 (en) 2011-08-19 2015-08-04 Emd Millipore Corporation Methods of reducing level of one or more impurities in a sample during protein purification
EP2578286A1 (en) * 2011-10-04 2013-04-10 Merck Patent GmbH Method and apparatus for chromatographic purification
KR102196009B1 (en) * 2011-10-25 2021-01-04 프로테나 바이오사이언시즈 리미티드 Antibody formulations and methods
WO2013075740A1 (en) * 2011-11-23 2013-05-30 Sanofi Antibody purification method

Also Published As

Publication number Publication date
HUE057119T2 (en) 2022-04-28
MX368177B (en) 2019-09-23
IL242408B (en) 2020-04-30
RU2662668C2 (en) 2018-07-26
AU2014264697A1 (en) 2015-11-26
TWI675041B (en) 2019-10-21
JP2016519137A (en) 2016-06-30
CA2911462A1 (en) 2014-11-13
CA2911462C (en) 2021-04-06
EP3301115B1 (en) 2021-11-10
HUE038741T2 (en) 2018-11-28
NO2994485T3 (en) 2018-06-09
KR102272851B1 (en) 2021-07-02
US10793622B2 (en) 2020-10-06
EP2994485A1 (en) 2016-03-16
AU2014264697B2 (en) 2019-08-22
DK2994485T3 (en) 2018-04-23
EP3301115A1 (en) 2018-04-04
RU2015151869A (en) 2017-06-13
CN105358572A (en) 2016-02-24
TWI631132B (en) 2018-08-01
TW201908331A (en) 2019-03-01
ES2665645T3 (en) 2018-04-26
CY1125281T1 (en) 2023-06-09
ES2905675T3 (en) 2022-04-11
US20160083454A1 (en) 2016-03-24
TW201522363A (en) 2015-06-16
MX2015015482A (en) 2016-03-21
CN116003579A (en) 2023-04-25
BR112015027812B1 (en) 2021-03-30
JP6463734B2 (en) 2019-02-06
DK3301115T3 (en) 2022-01-31
WO2014180852A1 (en) 2014-11-13
BR112015027812A2 (en) 2017-07-25
JP2019104735A (en) 2019-06-27
KR20160005047A (en) 2016-01-13
EP2994485B1 (en) 2018-01-10

Similar Documents

Publication Publication Date Title
IL242408B (en) Continuous multistep process for purifying antibodies
HK1217344A1 (en) Purification process for monoclonal antibodies
PT3004163T (en) Method for purifying antibody
SG11201603274YA (en) Process for producing methyl methacrylate
SG11201505204VA (en) Protein purification process
IL240753A0 (en) Methods for purifying antibodies
HK1217926A1 (en) Method for vacuum purification
IL245805A0 (en) Process for purifying 2,5-dichlorophenol
EP2987801A4 (en) Method for purifying antibody protein
IL239689A0 (en) Method for producing antibodies
SG11201607098SA (en) Antibody purification process
SG11201601819WA (en) Process for separating methacrolein
ZA201603797B (en) Novel purification processes
IL241287A0 (en) Processes for producing sovaprevir
HK1223378A1 (en) Purification process for pth pth
GB201302441D0 (en) Process
IL243937A0 (en) Process for the purification of diaminophenothiazinium compounds
AP2016009185A0 (en) Process for catalyst unloading facilitation
EP2993166A4 (en) Method for purifying dimethyl sulfoxide
HUE051329T2 (en) Process for purifying chlorine by fractionation
GB201307327D0 (en) Process
GB201300073D0 (en) Process
HK1217323A1 (en) Isohexide monotriflates and process for synthesis thereof
GB201301384D0 (en) Method for antibody production
GB201306308D0 (en) Novel process