MX361903B - Deteccion de secuencia de acidos nucleicos objetivo mediante ensayo de escision de pto e hibridacion de oligonucleotidos de señalizacion dependiente de la extension. - Google Patents
Deteccion de secuencia de acidos nucleicos objetivo mediante ensayo de escision de pto e hibridacion de oligonucleotidos de señalizacion dependiente de la extension.Info
- Publication number
- MX361903B MX361903B MX2014009403A MX2014009403A MX361903B MX 361903 B MX361903 B MX 361903B MX 2014009403 A MX2014009403 A MX 2014009403A MX 2014009403 A MX2014009403 A MX 2014009403A MX 361903 B MX361903 B MX 361903B
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6823—Release of bound markers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
La presente invención se refiere a la detección de una secuencia de ácidos nucleicos objetivo mediante un ensayo de PCE-SH (Escisión de PTO e Hibridación de Oligonucleótidos de Señalización Dependiente de la Extensión). La presente invención no utiliza sondas que se hibridan con secuencias de ácidos nucleicos objetivo para proporcionar señales objetivo. De manera interesante, la presente invención utiliza sondas (oligonucleótidos de señalización) que se hibridan con la cadena extendida formada de una manera dependiente del objetivo en la que la cadena extendida se sintetiza utilizando el CTO seleccionado artificialmente como plantillas.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR20120010681 | 2012-02-02 | ||
KR1020120028429A KR20130101952A (ko) | 2012-02-02 | 2012-03-20 | Pto 절단과 연장-의존적 혼성화를 이용한 타겟 핵산서열의 검출 |
PCT/KR2012/005284 WO2013005974A2 (ko) | 2011-07-05 | 2012-07-03 | 친환경 습도지시카드 |
Publications (2)
Publication Number | Publication Date |
---|---|
MX2014009403A MX2014009403A (es) | 2015-07-06 |
MX361903B true MX361903B (es) | 2018-12-18 |
Family
ID=49451920
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2014009403A MX361903B (es) | 2012-02-02 | 2012-07-03 | Deteccion de secuencia de acidos nucleicos objetivo mediante ensayo de escision de pto e hibridacion de oligonucleotidos de señalizacion dependiente de la extension. |
Country Status (15)
Country | Link |
---|---|
US (2) | US9868980B2 (es) |
EP (1) | EP2809797B1 (es) |
JP (1) | JP6005180B2 (es) |
KR (2) | KR20130101952A (es) |
CN (2) | CN106011234B (es) |
AU (2) | AU2012368436B2 (es) |
BR (1) | BR112014018940B1 (es) |
CA (1) | CA2862488C (es) |
IL (1) | IL233773B (es) |
MX (1) | MX361903B (es) |
NO (1) | NO20140948A1 (es) |
RU (1) | RU2608501C2 (es) |
SG (1) | SG11201404349YA (es) |
WO (1) | WO2013115442A1 (es) |
ZA (1) | ZA201405463B (es) |
Families Citing this family (36)
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MX340258B (es) | 2011-01-11 | 2016-07-01 | Seegene Inc | Detección de secuencias de ácido nucleico objetivo mediante ensayo de escisión y extensión del pto. |
EP2691543B1 (en) | 2011-03-29 | 2017-11-01 | Seegene, Inc. | Detection of target nucleic acid sequence by pto cleavage and extension-dependent cleavage |
US9850524B2 (en) | 2011-05-04 | 2017-12-26 | Seegene, Inc. | Detection of target nucleic acid sequences by PO cleavage and hybridization |
KR20130101952A (ko) | 2012-02-02 | 2013-09-16 | 주식회사 씨젠 | Pto 절단과 연장-의존적 혼성화를 이용한 타겟 핵산서열의 검출 |
CN104245959B (zh) | 2012-03-05 | 2017-10-13 | Seegene株式会社 | 基于探测和标记寡核苷酸切割及延伸试验的从靶核酸序列的核苷酸变异检测 |
WO2013157821A1 (en) | 2012-04-19 | 2013-10-24 | Seegene, Inc. | Detection of target nucleic acid sequence by pto cleavage and extension-dependent signaling oligonucleotide cleavage |
KR101572682B1 (ko) | 2013-05-30 | 2015-11-30 | 케이맥(주) | 바이오 물질의 실시간 정량 및 정성 분석 방법 |
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CN108823287B (zh) * | 2017-04-28 | 2019-04-12 | 厦门大学 | 一种检测靶核酸序列的方法 |
KR102380264B1 (ko) | 2017-08-31 | 2022-03-29 | 주식회사 씨젠 | 다이머-형성 프라이머 쌍을 이용한 구성요소의 성능 평가 |
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CN112592964A (zh) * | 2020-12-17 | 2021-04-02 | 厦门大学 | 用于进行核酸多重检测的方法 |
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KR20240056605A (ko) | 2021-09-30 | 2024-04-30 | 주식회사 씨젠 | 분자진단 시스템의 샘플 처리 및 분석 방법 |
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KR20130101952A (ko) | 2012-02-02 | 2013-09-16 | 주식회사 씨젠 | Pto 절단과 연장-의존적 혼성화를 이용한 타겟 핵산서열의 검출 |
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2012
- 2012-03-20 KR KR1020120028429A patent/KR20130101952A/ko unknown
- 2012-07-03 SG SG11201404349YA patent/SG11201404349YA/en unknown
- 2012-07-03 EP EP12867064.3A patent/EP2809797B1/en active Active
- 2012-07-03 BR BR112014018940-4A patent/BR112014018940B1/pt active IP Right Grant
- 2012-07-03 JP JP2014555469A patent/JP6005180B2/ja active Active
- 2012-07-03 AU AU2012368436A patent/AU2012368436B2/en active Active
- 2012-07-03 RU RU2014133695A patent/RU2608501C2/ru active
- 2012-07-03 CN CN201610320413.7A patent/CN106011234B/zh active Active
- 2012-07-03 US US14/374,567 patent/US9868980B2/en active Active
- 2012-07-03 CN CN201280071069.2A patent/CN104145029B/zh active Active
- 2012-07-03 WO PCT/KR2012/005281 patent/WO2013115442A1/en active Application Filing
- 2012-07-03 KR KR1020147022148A patent/KR101832867B1/ko active IP Right Grant
- 2012-07-03 MX MX2014009403A patent/MX361903B/es active IP Right Grant
- 2012-07-03 CA CA2862488A patent/CA2862488C/en active Active
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2014
- 2014-07-23 IL IL233773A patent/IL233773B/en active IP Right Grant
- 2014-07-24 ZA ZA2014/05463A patent/ZA201405463B/en unknown
- 2014-07-30 NO NO20140948A patent/NO20140948A1/no not_active Application Discontinuation
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2016
- 2016-07-13 AU AU2016204887A patent/AU2016204887B2/en active Active
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2017
- 2017-12-27 US US15/854,891 patent/US10731203B2/en active Active
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