MX357563B - Variantes de enzima quimosina mejoradas. - Google Patents

Variantes de enzima quimosina mejoradas.

Info

Publication number
MX357563B
MX357563B MX2014013333A MX2014013333A MX357563B MX 357563 B MX357563 B MX 357563B MX 2014013333 A MX2014013333 A MX 2014013333A MX 2014013333 A MX2014013333 A MX 2014013333A MX 357563 B MX357563 B MX 357563B
Authority
MX
Mexico
Prior art keywords
chymosine
improved
variant
enzyme variants
polypeptide
Prior art date
Application number
MX2014013333A
Other languages
English (en)
Other versions
MX2014013333A (es
Inventor
Jacobus Theodorus Dekker Petrus
Marcel Jong De René
Marinus Muijlwijk Cornelis
Original Assignee
Dsm Ip Assets Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dsm Ip Assets Bv filed Critical Dsm Ip Assets Bv
Publication of MX2014013333A publication Critical patent/MX2014013333A/es
Publication of MX357563B publication Critical patent/MX357563B/es

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C19/00—Cheese; Cheese preparations; Making thereof
    • A23C19/02—Making cheese curd
    • A23C19/032—Making cheese curd characterised by the use of specific microorganisms, or enzymes of microbial origin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C19/00—Cheese; Cheese preparations; Making thereof
    • A23C19/02—Making cheese curd
    • A23C19/032—Making cheese curd characterised by the use of specific microorganisms, or enzymes of microbial origin
    • A23C19/0326—Rennet produced by fermentation, e.g. microbial rennet; Rennet produced by genetic engineering
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C19/00—Cheese; Cheese preparations; Making thereof
    • A23C19/06—Treating cheese curd after whey separation; Products obtained thereby
    • A23C19/068—Particular types of cheese
    • A23C19/0684—Soft uncured Italian cheeses, e.g. Mozarella, Ricotta, Pasta filata cheese; Other similar stretched cheeses
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C19/00—Cheese; Cheese preparations; Making thereof
    • A23C19/06—Treating cheese curd after whey separation; Products obtained thereby
    • A23C19/068—Particular types of cheese
    • A23C19/072—Cheddar type or similar hard cheeses without eyes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6483—Chymosin (3.4.23.4), i.e. rennin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/23—Aspartic endopeptidases (3.4.23)
    • C12Y304/23004—Chymosin (3.4.23.4), i.e. rennin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C2220/00—Biochemical treatment
    • A23C2220/20—Treatment with microorganisms
    • A23C2220/202—Genetic engineering of microorganisms used in dairy technology
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C2250/00—Particular aspects related to cheese
    • A23C2250/15—Shredded non-dried cheese

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Dairy Products (AREA)

Abstract

La presente invención se refiere a un polipéptido variante que tiene actividad quimosina, en que la variante tiene una secuencia de aminoácidos que, cuando se alinea con la quimosina que comprende la secuencia expuesta en SEC ID N°: 2, comprende al menos una sustitución de un resto de aminoácido correspondiente a cualquiera de los aminoácidos 2, 22, 40, 48, 50, 51, 53, 61, 62, 76, 88, 98, 99, 109, 112, 117, 125, 126, 135, 144, 160, 161, 163, 187, 189, 194, 200, 201, 202, 203, 221, 223, 240, 242, 244, 254, 267, 271, 273, 278, 280, 284, 289, 292, 294 o 295 definiéndose dichas posiciones con referencia a SEC ID N°: 2 y en que la variante tiene una o más propiedades alteradas en comparación con un polipéptido de referencia que tiene actividad quimosina. Dicho polipéptido variante puede usarse en la preparación de un queso.
MX2014013333A 2012-05-03 2013-05-03 Variantes de enzima quimosina mejoradas. MX357563B (es)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US201261642095P 2012-05-03 2012-05-03
EP12166673 2012-05-03
US201261745063P 2012-12-21 2012-12-21
EP12199178 2012-12-21
EP12199277 2012-12-21
PCT/EP2013/059315 WO2013164479A2 (en) 2012-05-03 2013-05-03 Improved enzyme variants

Publications (2)

Publication Number Publication Date
MX2014013333A MX2014013333A (es) 2015-02-10
MX357563B true MX357563B (es) 2018-07-13

Family

ID=49514227

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2014013329A MX355170B (es) 2012-05-03 2013-05-03 Variantes de enzimas mejoradas.
MX2014013333A MX357563B (es) 2012-05-03 2013-05-03 Variantes de enzima quimosina mejoradas.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
MX2014013329A MX355170B (es) 2012-05-03 2013-05-03 Variantes de enzimas mejoradas.

Country Status (12)

Country Link
US (3) US10167463B2 (es)
EP (3) EP2844751B1 (es)
AU (2) AU2013255749A1 (es)
BR (2) BR112014027371A2 (es)
CA (2) CA2872309C (es)
DK (2) DK2844750T3 (es)
EA (2) EA038324B1 (es)
HK (2) HK1207880A1 (es)
LT (2) LT2844750T (es)
MX (2) MX355170B (es)
NZ (1) NZ701125A (es)
WO (2) WO2013164481A1 (es)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL2855674T5 (pl) 2012-05-25 2023-03-27 Chr. Hansen A/S Warianty chymozyny o ulepszonych właściwościach ścinania mleka
CN111944791A (zh) * 2014-02-26 2020-11-17 科·汉森有限公司 具有提高的凝乳特性的凝乳酶变体
KR20170115614A (ko) 2015-02-10 2017-10-17 시에이치알. 한센 에이/에스 개선된 응유 특성을 갖는 키모신 배합물
EP3310914B1 (en) 2015-06-22 2022-04-06 Chr. Hansen A/S Variants of chymosin with improved properties
EP3344049A1 (en) * 2015-08-31 2018-07-11 Chr. Hansen A/S Variants of chymosin with improved properties
EP3143879A1 (en) 2015-09-18 2017-03-22 DSM IP Assets B.V. Yogurt with reduced ripening
GB201517601D0 (en) * 2015-10-06 2015-11-18 Dale Farm Ltd Improvements relating to cheese making
EP3457860A1 (en) 2016-05-19 2019-03-27 Chr. Hansen A/S Variants of chymosin with improved milk-clotting properties
CN109688825A (zh) * 2016-05-19 2019-04-26 科·汉森有限公司 具有改善的凝乳特性的凝乳酶的变体
CN106754840B (zh) * 2016-12-22 2019-06-21 江南大学 一种酶活及热稳定性改善的凝乳酶突变体
US11896022B2 (en) * 2018-01-11 2024-02-13 Chr. Hansen A/S Interactions between cultures, coagulants and technology to increase cheese yields
CN109182311B (zh) * 2018-10-11 2021-07-27 山东隆科特酶制剂有限公司 一种用于沂蒙黑山羊乳奶酪的凝乳酶
WO2023001832A1 (en) 2021-07-20 2023-01-26 Chr. Hansen A/S Composition for clotting milk, methods and uses thereof
WO2023194285A2 (en) 2022-04-06 2023-10-12 Chr. Hansen A/S Aspartic protease, methods and uses thereof

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2354775T5 (es) * 2000-11-06 2021-12-23 Chr Hansen As Método de producción de quimosina no bovina y uso de la misma
WO2008008766A2 (en) * 2006-07-10 2008-01-17 Ohio University Co-expression of proline hydroxylases to facilitate hyp-glycosylation of proteins expressed and secreted in plant cells
EP3763220A1 (en) * 2007-02-13 2021-01-13 Chr. Hansen A/S Coagulation of milk

Also Published As

Publication number Publication date
CA2872309C (en) 2021-03-02
WO2013164479A2 (en) 2013-11-07
US10167463B2 (en) 2019-01-01
US20180030427A1 (en) 2018-02-01
EP3450552A2 (en) 2019-03-06
HK1207881A1 (en) 2016-02-12
LT2844750T (lt) 2018-09-10
EA201401211A1 (ru) 2015-05-29
EP2844751B1 (en) 2018-10-17
DK2844751T3 (en) 2019-02-04
WO2013164481A1 (en) 2013-11-07
MX2014013329A (es) 2015-02-10
EP2844750B1 (en) 2018-06-20
CA2872132C (en) 2020-06-16
AU2013255749A1 (en) 2014-11-06
BR112014027283A2 (pt) 2017-08-08
CA2872309A1 (en) 2013-11-07
BR112014027371A2 (pt) 2017-08-08
EA201401209A1 (ru) 2015-05-29
CA2872132A1 (en) 2013-11-07
NZ701125A (en) 2016-11-25
HK1207880A1 (en) 2016-02-12
DK2844750T3 (en) 2018-10-01
EP3450552A3 (en) 2019-05-08
US10253305B2 (en) 2019-04-09
EP2844751A2 (en) 2015-03-11
EA035275B1 (ru) 2020-05-22
AU2013255747A1 (en) 2014-11-06
MX2014013333A (es) 2015-02-10
EP2844750A1 (en) 2015-03-11
WO2013164479A3 (en) 2013-12-19
MX355170B (es) 2018-04-05
EA038324B1 (ru) 2021-08-10
LT2844751T (lt) 2019-02-11
US9822352B2 (en) 2017-11-21
US20150118356A1 (en) 2015-04-30
US20150140169A1 (en) 2015-05-21

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