PL2855674T5 - Warianty chymozyny o ulepszonych właściwościach ścinania mleka - Google Patents

Warianty chymozyny o ulepszonych właściwościach ścinania mleka

Info

Publication number
PL2855674T5
PL2855674T5 PL13727539.2T PL13727539T PL2855674T5 PL 2855674 T5 PL2855674 T5 PL 2855674T5 PL 13727539 T PL13727539 T PL 13727539T PL 2855674 T5 PL2855674 T5 PL 2855674T5
Authority
PL
Poland
Prior art keywords
chymosin
variants
improved milk
clotting properties
clotting
Prior art date
Application number
PL13727539.2T
Other languages
English (en)
Other versions
PL2855674T3 (pl
Inventor
Johannes Maarten Van Den Brink
Martin Lund
Jonas Jacobsen
Sari Charlotte HANSEN
Iben Jeppesen
Original Assignee
Chr. Hansen A/S
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=48577692&utm_source=***_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=PL2855674(T5) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Chr. Hansen A/S filed Critical Chr. Hansen A/S
Publication of PL2855674T3 publication Critical patent/PL2855674T3/pl
Publication of PL2855674T5 publication Critical patent/PL2855674T5/pl

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478Aspartic endopeptidases (3.4.23)
    • C12N9/6483Chymosin (3.4.23.4), i.e. rennin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y304/00Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/23Aspartic endopeptidases (3.4.23)
    • C12Y304/23004Chymosin (3.4.23.4), i.e. rennin
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C11/00Milk substitutes, e.g. coffee whitener compositions
    • A23C11/02Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins
    • A23C11/10Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins containing or not lactose but no other milk components as source of fats, carbohydrates or proteins
    • A23C11/103Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins containing or not lactose but no other milk components as source of fats, carbohydrates or proteins containing only proteins from pulses, oilseeds or nuts, e.g. nut milk
    • A23C11/106Addition of, or treatment with, microorganisms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C19/00Cheese; Cheese preparations; Making thereof
    • A23C19/02Making cheese curd
    • A23C19/032Making cheese curd characterised by the use of specific microorganisms, or enzymes of microbial origin
    • A23C19/0326Rennet produced by fermentation, e.g. microbial rennet; Rennet produced by genetic engineering
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C20/00Cheese substitutes
    • A23C20/02Cheese substitutes containing neither milk components, nor caseinate, nor lactose, as sources of fats, proteins or carbohydrates
    • A23C20/025Cheese substitutes containing neither milk components, nor caseinate, nor lactose, as sources of fats, proteins or carbohydrates mainly containing proteins from pulses or oilseeds
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00Milk preparations; Milk powder or milk powder preparations
    • A23C9/12Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/1203Addition of, or treatment with, enzymes or microorganisms other than lactobacteriaceae
    • A23C9/1209Proteolytic or milk coagulating enzymes, e.g. trypsine
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/189Enzymes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L11/00Pulses, i.e. fruits of leguminous plants, for production of food; Products from legumes; Preparation or treatment thereof
    • A23L11/40Pulse curds
    • A23L11/45Soy bean curds, e.g. tofu
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L11/00Pulses, i.e. fruits of leguminous plants, for production of food; Products from legumes; Preparation or treatment thereof
    • A23L11/60Drinks from legumes, e.g. lupine drinks
    • A23L11/65Soy drinks
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00Production
    • C12N2330/50Biochemical production, i.e. in a transformed host cell

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Polymers & Plastics (AREA)
  • Organic Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Nutrition Science (AREA)
  • Agronomy & Crop Science (AREA)
  • Botany (AREA)
  • Medicinal Chemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Animal Husbandry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Peptides Or Proteins (AREA)
  • Dairy Products (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Fodder In General (AREA)
  • General Preparation And Processing Of Foods (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
PL13727539.2T 2012-05-25 2013-05-22 Warianty chymozyny o ulepszonych właściwościach ścinania mleka PL2855674T5 (pl)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP12169503 2012-05-25
PCT/EP2013/060460 WO2013174840A1 (en) 2012-05-25 2013-05-22 Variants of chymosin with improved milk-clotting properties
EP13727539.2A EP2855674B2 (en) 2012-05-25 2013-05-22 Variants of chymosin with improved milk-clotting properties

Publications (2)

Publication Number Publication Date
PL2855674T3 PL2855674T3 (pl) 2019-11-29
PL2855674T5 true PL2855674T5 (pl) 2023-03-27

Family

ID=48577692

Family Applications (2)

Application Number Title Priority Date Filing Date
PL13727539.2T PL2855674T5 (pl) 2012-05-25 2013-05-22 Warianty chymozyny o ulepszonych właściwościach ścinania mleka
PL19174281T PL3604509T3 (pl) 2012-05-25 2013-05-22 Warianty chymozyny o ulepszonych właściwościach ścinania mleka

Family Applications After (1)

Application Number Title Priority Date Filing Date
PL19174281T PL3604509T3 (pl) 2012-05-25 2013-05-22 Warianty chymozyny o ulepszonych właściwościach ścinania mleka

Country Status (19)

Country Link
US (2) US9930899B2 (pl)
EP (3) EP2855674B2 (pl)
JP (3) JP2015518718A (pl)
KR (2) KR102419515B1 (pl)
CN (2) CN104487572B (pl)
AU (3) AU2013265343B2 (pl)
BR (2) BR122021019895B1 (pl)
CA (3) CA3100606C (pl)
DK (2) DK3604509T3 (pl)
ES (1) ES2741723T5 (pl)
FI (1) FI2855674T4 (pl)
HK (1) HK1202894A1 (pl)
MX (3) MX366610B (pl)
NZ (4) NZ702030A (pl)
PL (2) PL2855674T5 (pl)
RU (2) RU2683385C2 (pl)
TR (1) TR201910420T4 (pl)
UA (2) UA118956C2 (pl)
WO (1) WO2013174840A1 (pl)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
UA118956C2 (uk) 2012-05-25 2019-04-10 Кр. Хансен А/С Варіант хімозину з поліпшеними молокозгортальними властивостями
US10982204B2 (en) * 2014-02-26 2021-04-20 Chr. Hansen A/S Variants of chymosin with improved milk-clotting properties
RU2741804C2 (ru) 2015-02-10 2021-01-28 Кхр. Хансен А/С Смеси химозинов с улучшенными молокосвертывающими свойствами
EP3310914B1 (en) * 2015-06-22 2022-04-06 Chr. Hansen A/S Variants of chymosin with improved properties
KR20180038569A (ko) * 2015-08-31 2018-04-16 시에이치알. 한센 에이/에스 특성이 개선된 키모신 변이체
EP3457860A1 (en) 2016-05-19 2019-03-27 Chr. Hansen A/S Variants of chymosin with improved milk-clotting properties
US10954505B2 (en) * 2016-05-19 2021-03-23 Chr. Hansen A/S Variants of chymosin with improved milk-clotting properties
CN106754840B (zh) * 2016-12-22 2019-06-21 江南大学 一种酶活及热稳定性改善的凝乳酶突变体
WO2019138121A1 (en) 2018-01-15 2019-07-18 Chr. Hansen A/S Fermented milk product and preparation thereof using phospholipase
CN111172139B (zh) * 2020-02-27 2021-11-30 武汉轻工大学 一种小牛皱胃凝乳酶及基因与菌株和应用
CN113801867B (zh) * 2021-11-18 2022-03-18 中国农业科学院生物技术研究所 骆驼凝乳酶原突变蛋白及其编码基因和应用
WO2023194285A2 (en) 2022-04-06 2023-10-12 Chr. Hansen A/S Aspartic protease, methods and uses thereof

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0123928A3 (en) 1983-03-31 1986-02-05 Codon Genetic Engineering Laboratories Recombinant dna coding for a polypeptide displaying milk clotting activity
US7390936B1 (en) 1999-08-23 2008-06-24 Sembiosys Genetics Inc. Commercial production of chymosin in plants
RU2192137C2 (ru) * 2000-08-07 2002-11-10 Открытое акционерное общество "Уфамолагропром" Способ производства сыра
ATE486933T1 (de) 2000-11-06 2010-11-15 Chr Hansen As Verfahren zur herstellung von nicht aus dem rind stammendem chymosin und dessen anwendung
CA2501295A1 (en) 2002-10-02 2004-04-15 Jack Nguyen Methods of generating and screening for proteases with altered specificity
BRPI0412007A (pt) 2003-06-27 2006-08-15 Bioren Inc mutagênese look-through
US7482148B2 (en) 2004-03-30 2009-01-27 Sudershan Biotech Ltd. Recombinant calf-chymosin and a process for producing the same
WO2008098973A1 (en) 2007-02-13 2008-08-21 Chr. Hansen A/S Coagulation of milk
JP2010046034A (ja) 2008-08-22 2010-03-04 Toyota Central R&D Labs Inc 変異体のスクリーニング方法
NZ595078A (en) 2009-03-24 2012-07-27 Meito Sangyo Kk Improved type milk-clotting protease derived from a microorganism
CA2872132C (en) 2012-05-03 2020-06-16 Dsm Ip Assets B.V. Improved enzyme variants
UA118956C2 (uk) 2012-05-25 2019-04-10 Кр. Хансен А/С Варіант хімозину з поліпшеними молокозгортальними властивостями
US10982204B2 (en) 2014-02-26 2021-04-20 Chr. Hansen A/S Variants of chymosin with improved milk-clotting properties
RU2741804C2 (ru) 2015-02-10 2021-01-28 Кхр. Хансен А/С Смеси химозинов с улучшенными молокосвертывающими свойствами
EP3310914B1 (en) 2015-06-22 2022-04-06 Chr. Hansen A/S Variants of chymosin with improved properties
KR20180038569A (ko) 2015-08-31 2018-04-16 시에이치알. 한센 에이/에스 특성이 개선된 키모신 변이체
EP3457860A1 (en) 2016-05-19 2019-03-27 Chr. Hansen A/S Variants of chymosin with improved milk-clotting properties
US10954505B2 (en) 2016-05-19 2021-03-23 Chr. Hansen A/S Variants of chymosin with improved milk-clotting properties

Also Published As

Publication number Publication date
UA126330C2 (uk) 2022-09-21
EP2855674A1 (en) 2015-04-08
RU2683385C2 (ru) 2019-03-28
EP2855674B1 (en) 2019-05-15
CN110714001A (zh) 2020-01-21
KR20210048580A (ko) 2021-05-03
FI2855674T4 (fi) 2023-01-13
AU2019203589B2 (en) 2021-11-04
ES2741723T3 (es) 2020-02-12
BR122021019895B1 (pt) 2022-10-25
BR112014029012B1 (pt) 2022-05-03
ES2741723T5 (es) 2023-01-23
MX2014014300A (es) 2015-02-12
US20150173383A1 (en) 2015-06-25
MX2020011941A (es) 2021-01-15
CA3201812A1 (en) 2013-11-28
CN104487572A (zh) 2015-04-01
US10806157B2 (en) 2020-10-20
EP3929288A1 (en) 2021-12-29
CA2874214A1 (en) 2013-11-28
AU2019203589A1 (en) 2019-06-13
TR201910420T4 (tr) 2019-08-21
KR102419515B1 (ko) 2022-07-11
KR20150015014A (ko) 2015-02-09
HK1202894A1 (en) 2015-10-09
US20180317510A1 (en) 2018-11-08
RU2019108268A (ru) 2019-04-23
JP2018148926A (ja) 2018-09-27
DK2855674T4 (da) 2022-11-21
CN104487572B (zh) 2019-12-13
CA3100606C (en) 2023-08-01
RU2019108268A3 (pl) 2021-11-29
WO2013174840A1 (en) 2013-11-28
NZ702030A (en) 2016-11-25
PL2855674T3 (pl) 2019-11-29
JP2015518718A (ja) 2015-07-06
MX2019008255A (es) 2020-01-15
KR102245756B1 (ko) 2021-04-29
CA2874214C (en) 2021-02-16
EP2855674B2 (en) 2022-11-02
RU2014152723A (ru) 2016-07-20
JP2020058367A (ja) 2020-04-16
EP3604509B1 (en) 2021-03-31
US9930899B2 (en) 2018-04-03
EP3604509A1 (en) 2020-02-05
AU2013265343B2 (en) 2019-02-21
UA118956C2 (uk) 2019-04-10
PL3604509T3 (pl) 2021-09-13
AU2022200663A1 (en) 2022-02-24
NZ742745A (en) 2019-12-20
BR112014029012A2 (pt) 2017-06-27
AU2013265343A1 (en) 2014-12-04
DK2855674T3 (da) 2019-06-24
CA3100606A1 (en) 2013-11-28
MX366610B (es) 2019-07-16
DK3604509T3 (da) 2021-05-25
NZ725398A (en) 2018-05-25
NZ742660A (en) 2019-12-20

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