MX353840B - Deteccion de acidos nucleicos. - Google Patents

Deteccion de acidos nucleicos.

Info

Publication number
MX353840B
MX353840B MX2014010007A MX2014010007A MX353840B MX 353840 B MX353840 B MX 353840B MX 2014010007 A MX2014010007 A MX 2014010007A MX 2014010007 A MX2014010007 A MX 2014010007A MX 353840 B MX353840 B MX 353840B
Authority
MX
Mexico
Prior art keywords
detection
nucleic acids
methods
oligonucleotides
amplifying
Prior art date
Application number
MX2014010007A
Other languages
English (en)
Other versions
MX2014010007A (es
Inventor
Velyian Todd Alison
Mokany Elisa
Lonergan Dina
Walker Samantha
Donald Cortny
Yeen Tan Lit
Original Assignee
Speedx Pty Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AU2012900624A external-priority patent/AU2012900624A0/en
Application filed by Speedx Pty Ltd filed Critical Speedx Pty Ltd
Publication of MX2014010007A publication Critical patent/MX2014010007A/es
Publication of MX353840B publication Critical patent/MX353840B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

La presente invención se refiere a oligonucleótidos modificados y métodos para su uso en la detección de ácidos nucleicos. Los oligonucleótidos y método encuentran aplicación particular en amplificar y/o detectar áreas de variación genética en las secuencias de ácidos nucleicos objetivo.
MX2014010007A 2012-02-20 2013-02-20 Deteccion de acidos nucleicos. MX353840B (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
AU2012900624A AU2012900624A0 (en) 2012-02-20 Detection of Nucleic Acids
AU2012902218A AU2012902218A0 (en) 2012-05-29 Detection of nucleic acids
PCT/AU2013/000149 WO2013123552A1 (en) 2012-02-20 2013-02-20 Detection of nucleic acids

Publications (2)

Publication Number Publication Date
MX2014010007A MX2014010007A (es) 2014-09-12
MX353840B true MX353840B (es) 2018-01-31

Family

ID=49004851

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2014010007A MX353840B (es) 2012-02-20 2013-02-20 Deteccion de acidos nucleicos.

Country Status (18)

Country Link
US (3) US9963738B2 (es)
EP (1) EP2817421B1 (es)
JP (2) JP6276201B2 (es)
KR (1) KR102085119B1 (es)
CN (1) CN104245958B (es)
AU (1) AU2013202920B9 (es)
BR (1) BR112014020422B1 (es)
CA (1) CA2863808C (es)
DK (1) DK2817421T3 (es)
ES (1) ES2660248T3 (es)
HK (1) HK1199743A1 (es)
IL (1) IL233958A (es)
MX (1) MX353840B (es)
NO (1) NO2817421T3 (es)
NZ (1) NZ628883A (es)
SG (1) SG11201405012YA (es)
WO (1) WO2013123552A1 (es)
ZA (1) ZA201406410B (es)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112014005505B1 (pt) 2011-09-09 2021-01-12 Speedx Pty Ltd. substrato de polinucleotídeo isolado para uma enzima de ácido nucleico multi-componente (mnazima) ou dnazima, método para detectar a presença de pelo menos um alvo, kit e montagem
CN104245958B (zh) 2012-02-20 2018-05-22 斯比戴克斯私人有限公司 核酸的检测
AU2013202354A1 (en) * 2012-06-18 2014-01-16 Speedx Pty Ltd Target detection and signal amplification
EP2953958B1 (en) 2013-02-07 2018-04-11 Rutgers, the State University of New Jersey Highly selective nucleic acid amplification primers
CN103540660B (zh) * 2013-10-10 2015-10-28 中国人民解放军疾病预防控制所 基于TaqMan探针的环介导恒温扩增法及其专用LAMP引物与试剂盒
JP6590831B2 (ja) * 2014-04-25 2019-10-16 ディーエヌエーイー グループ ホールディングス リミテッド 配列決定方法
KR101631114B1 (ko) * 2014-06-13 2016-06-16 서울대학교산학협력단 육종감별을 위한 등온증폭반응용 프라이머 세트 및 이를 이용한 육종 감별 방법
WO2016077291A1 (en) * 2014-11-10 2016-05-19 University Of Pittsburgh - Of The Commonwealth System Of Higher Education Isothermal amplification assay for the detection of short nucleic acid sequences
CN107614697A (zh) * 2015-02-26 2018-01-19 奥斯瑞根公司 用于提高突变评估准确性的方法和装置
CN104845967B (zh) * 2015-04-15 2020-12-11 苏州新海生物科技股份有限公司 寡聚核苷酸片段及使用其的选择性扩增目标核酸序列变异体的方法及应用
AU2016296594B2 (en) * 2015-07-17 2020-07-09 Luminex Corporation Methods and compositions for catalytic assays
EP3665305A1 (en) 2017-08-10 2020-06-17 Katholieke Universiteit Leuven Nucleic acid enzyme sensor
GB201713251D0 (en) * 2017-08-18 2017-10-04 Lgc Genomics Ltd Methods and kits for detection of nucleic acid molecules
KR101987388B1 (ko) * 2017-10-19 2019-06-12 경상대학교산학협력단 분자비콘을 포함하는 비브리오균의 등온비색 검출용 조성물 및 이의 용도
CN107966438B (zh) * 2017-10-27 2020-11-24 中国农业大学 一种基于锌的功能核酸的耐高盐传感器及其应用
WO2019150414A1 (ja) * 2018-01-30 2019-08-08 学校法人 慶應義塾 核酸の検出方法
BR112021002398A2 (pt) * 2018-08-09 2021-05-04 Speedx Pty Ltd método para determinar a presença ou ausência de primeiro e segundo alvos em uma amostra e composição
CN109097468A (zh) * 2018-08-14 2018-12-28 南通市第人民医院 Opcml基因启动子甲基化水平的检测试剂盒及其应用
CN110452988A (zh) * 2019-08-27 2019-11-15 武汉友芝友医疗科技股份有限公司 检测esr1基因突变的引物组、试剂、试剂盒和方法
AU2021367997A1 (en) 2020-10-29 2023-06-08 Biocartis Nv Generic cartridge and method for multiplex nucleic acid detection
CN112662804B (zh) * 2021-01-25 2022-05-10 中国水稻研究所 一种检测稻瘟病菌无毒基因AvrPi9致病性变异的引物组、试剂盒及方法
CA3206960A1 (en) * 2021-02-02 2022-08-11 Rhodx, Inc. Synthetic polynucleotides and methods for selectively amplifying alleles
CN112852934B (zh) * 2021-04-16 2022-02-18 武汉友芝友医疗科技股份有限公司 检测基因甲基化的引物、试剂和试剂盒
WO2023067110A1 (en) * 2021-10-20 2023-04-27 Tataa Biocenter Ab Methods and compositions for detection of mutant nucleic acid sequences

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040121374A1 (en) * 2002-10-02 2004-06-24 Yoshihide Iwaki Method for detecting single nucleotide polymorphisms
WO2006095981A1 (en) * 2005-03-05 2006-09-14 Seegene, Inc. Processes using dual specificity oligonucleotide and dual specificity oligonucleotide
EP2385141B1 (en) * 2005-10-07 2013-08-07 SpeeDx Pty Ltd Multicomponent nucleic acid enzymes and methods for their use
ES2520023T3 (es) 2006-10-06 2014-11-11 Speedx Pty Ltd Interruptores moleculares y métodos para su uso
ES2425066T3 (es) 2007-04-05 2013-10-11 Speedx Pty Ltd Enzimas y complejos de ácidos nucleicos y métodos para su uso
JP2010035532A (ja) * 2008-08-08 2010-02-18 Canon Inc アレル特異的ｐｃｒ法
CN102459630B (zh) 2009-06-29 2014-04-30 株式会社东芝 样本分析方法和用于该方法的检测试剂盒
JP5115825B2 (ja) * 2009-11-06 2013-01-09 独立行政法人農業・食品産業技術総合研究機構 イグサ品種「ひのみどり」の識別マーカー
WO2011078441A1 (en) * 2009-12-21 2011-06-30 Seegene, Inc. Tsg primer target detection
CN102471771B (zh) * 2010-01-21 2016-01-20 爱科来株式会社 靶序列的扩增方法、多态性检测方法以及其中使用的试剂
CN104245958B (zh) 2012-02-20 2018-05-22 斯比戴克斯私人有限公司 核酸的检测

Also Published As

Publication number Publication date
MX2014010007A (es) 2014-09-12
CA2863808C (en) 2020-07-21
EP2817421A1 (en) 2014-12-31
US10724080B2 (en) 2020-07-28
AU2013202920A1 (en) 2013-09-05
US20190024160A1 (en) 2019-01-24
AU2013202920A9 (en) 2015-08-20
US20210108261A1 (en) 2021-04-15
AU2013202920B9 (en) 2015-10-01
ZA201406410B (en) 2015-09-30
JP2015511818A (ja) 2015-04-23
NZ628883A (en) 2015-07-31
DK2817421T3 (en) 2018-03-05
NO2817421T3 (es) 2018-05-12
BR112014020422B1 (pt) 2021-12-28
WO2013123552A9 (en) 2014-08-28
US9963738B2 (en) 2018-05-08
JP6836302B2 (ja) 2021-02-24
JP6276201B2 (ja) 2018-02-07
WO2013123552A1 (en) 2013-08-29
US11802308B2 (en) 2023-10-31
ES2660248T3 (es) 2018-03-21
US20160348161A1 (en) 2016-12-01
KR102085119B1 (ko) 2020-03-05
EP2817421B1 (en) 2017-12-13
CN104245958B (zh) 2018-05-22
AU2013202920B2 (en) 2015-09-17
IL233958A0 (en) 2014-09-30
KR20140139497A (ko) 2014-12-05
CN104245958A (zh) 2014-12-24
BR112014020422A2 (pt) 2018-10-30
JP2018075033A (ja) 2018-05-17
CA2863808A1 (en) 2013-08-29
EP2817421A4 (en) 2016-01-06
SG11201405012YA (en) 2014-09-26
IL233958A (en) 2017-03-30
HK1199743A1 (en) 2015-07-17

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