BR112012030813A2 - "métodos e kits para detecção in situ de sequências de nucleotídeo" - Google Patents

"métodos e kits para detecção in situ de sequências de nucleotídeo"

Info

Publication number
BR112012030813A2
BR112012030813A2 BR112012030813A BR112012030813A BR112012030813A2 BR 112012030813 A2 BR112012030813 A2 BR 112012030813A2 BR 112012030813 A BR112012030813 A BR 112012030813A BR 112012030813 A BR112012030813 A BR 112012030813A BR 112012030813 A2 BR112012030813 A2 BR 112012030813A2
Authority
BR
Brazil
Prior art keywords
methods
kits
nucleotide sequences
situ detection
relates
Prior art date
Application number
BR112012030813A
Other languages
English (en)
Inventor
Aurich Costa Joan
Original Assignee
Cellay Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cellay Inc filed Critical Cellay Inc
Publication of BR112012030813A2 publication Critical patent/BR112012030813A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

métodos e kits para detecção in situ de sequências de nucleotídeo. a presente invenção refere-se a métodos de hibridização in situ compreendendo uma etapa de hibridização em temperatura ambiente para detectar um ácido nucleico alvo em uma amostra biológica. a invenção também refere-se a kits para realizar tais métodos.
BR112012030813A 2010-06-03 2011-06-02 "métodos e kits para detecção in situ de sequências de nucleotídeo" BR112012030813A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US35106410P 2010-06-03 2010-06-03
PCT/US2011/038934 WO2011153354A1 (en) 2010-06-03 2011-06-02 Methods and kits for in situ detection of nucleotide sequences

Publications (1)

Publication Number Publication Date
BR112012030813A2 true BR112012030813A2 (pt) 2017-06-20

Family

ID=44486847

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112012030813A BR112012030813A2 (pt) 2010-06-03 2011-06-02 "métodos e kits para detecção in situ de sequências de nucleotídeo"

Country Status (5)

Country Link
US (1) US20130203055A1 (pt)
EP (1) EP2576830B1 (pt)
BR (1) BR112012030813A2 (pt)
DK (1) DK2576830T3 (pt)
WO (1) WO2011153354A1 (pt)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
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CN102046808B (zh) 2008-05-27 2017-05-17 丹麦达科有限公司 使用新的杂交缓冲液用于检测染色体畸变的组合物和方法
US9303287B2 (en) 2009-02-26 2016-04-05 Dako Denmark A/S Compositions and methods for RNA hybridization applications
EP2761028A1 (en) 2011-09-30 2014-08-06 Dako Denmark A/S Hybridization compositions and methods using formamide
EP3252173A1 (en) 2011-10-21 2017-12-06 Dako Denmark A/S Hybridization compositions and methods
CN104145027A (zh) 2011-12-12 2014-11-12 塞雷公司 用于室温原位检测生物样品中的目标核酸的方法和试剂盒
EP3066214B1 (en) 2013-11-07 2019-10-02 Cellay, Inc. Methods and kits for detecting a prostate carcinoma and predicting disease outcomes for prostate cancers
US10525076B2 (en) 2015-02-20 2020-01-07 Rosalind Franklin University Of Medicine And Science Antisense compounds targeting genes associated with cystic fibrosis
EP3259356B1 (en) * 2015-02-20 2021-12-01 Rosalind Franklin University of Medicine and Science Antisense compounds targeting genes associated with cystic fibrosis
WO2017041005A1 (en) 2015-09-03 2017-03-09 Abbott Molecular Inc. Hybridization buffers comprising an alkyl diester
WO2017119930A1 (en) 2016-01-08 2017-07-13 Abbott Molecular Inc. Hybridization buffers comprising guanidinium thiocyanate
KR102187291B1 (ko) 2016-11-21 2020-12-07 나노스트링 테크놀로지스, 인크. 화학적 조성물 및 이것을 사용하는 방법
JP2021523723A (ja) 2018-05-14 2021-09-09 ナノストリング テクノロジーズ，インコーポレイティド 化学的組成物とそれを利用する方法
US20230151415A1 (en) * 2020-04-07 2023-05-18 Advanced Cell Diagnostics, Inc. Methods for detecting target nucleic acids by in situ hybridization and a kit thereof
CN113063646B (zh) * 2021-03-27 2022-06-21 复旦大学 一种细胞固定剂、细胞固定方法和应用

Family Cites Families (15)

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Publication number Priority date Publication date Assignee Title
US5753437A (en) * 1987-10-13 1998-05-19 The United States Of America As Represented By The Department Of Health & Human Services Method of diagnosing cancer susceptibility or metastatic potential
US5082830A (en) 1988-02-26 1992-01-21 Enzo Biochem, Inc. End labeled nucleotide probe
US5714327A (en) 1990-07-19 1998-02-03 Kreatech Diagnostics Platinum-containing compounds, methods for their preparation and applications thereof
NL9001639A (nl) 1990-07-19 1992-02-17 Amc Amsterdam Pt-houdende verbinding, werkwijze voor de bereiding ervan, alsmede toepassing van dergelijke verbindingen.
US5719024A (en) 1993-08-18 1998-02-17 Applied Spectral Imaging Ltd. Method for chromosome classification by decorrelation statistical analysis and hardware therefore
ATE246696T1 (de) 1995-05-09 2003-08-15 Kreatech Biotech Bv Verfahren zur herstellung von linkern zwischen markierungsgruppen und bio-organischen molekülen auf der basis von platin für die markierung von bio-organischen molekülen zur entdeckung interessanter biologischer substanzen
WO1997004133A1 (en) * 1995-07-21 1997-02-06 Regents Of The University Of Minnesota Analysis of alpha integrins for the diagnosis of diabetic nephropathy
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US20020197656A1 (en) * 1999-12-17 2002-12-26 Ronghao Li Cell arrays and the uses thereof
US20040115692A1 (en) * 2000-04-03 2004-06-17 Cytyc Corporation Methods, compositions and apparatuses for detecting a target in a preservative solution
US6617148B2 (en) * 2000-06-29 2003-09-09 E. I. Du Pont De Nemours And Company Natural promoters for gene expression and metabolic monitoring in bacillus species
WO2002069898A2 (en) 2001-03-02 2002-09-12 Stratagene Compositions and methods using platinum compounds for nucleic acid labeling
JP2006505273A (ja) * 2002-11-05 2006-02-16 ロスカンプ リサーチ エルエルシー トゥレット症候群および関連異常における有機アニオン輸送ポリペプチド関連タンパク質−４（ｏａｔｐｒｐ４）遺伝子
EP1766072B1 (en) * 2004-06-03 2010-08-04 AdvanDx, Inc. Hybridization of pna probes in alcohol solutions
US20090036664A1 (en) * 2007-07-31 2009-02-05 Brian Jon Peter Complex oligonucleotide primer mix

Also Published As

Publication number Publication date
WO2011153354A8 (en) 2013-01-03
WO2011153354A1 (en) 2011-12-08
EP2576830B1 (en) 2014-08-27
EP2576830A1 (en) 2013-04-10
US20130203055A1 (en) 2013-08-08
DK2576830T3 (en) 2014-12-08

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Legal Events

Date Code Title Description
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]
B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: EM VIRTUDE DO ARQUIVAMENTO PUBLICADO NA RPI 2465 DE 03-04-2018 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDO O ARQUIVAMENTO DO PEDIDO DE PATENTE, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.