MX353478B - Ensamblaje combinacional de sitios multiples, a la medida. - Google Patents

Ensamblaje combinacional de sitios multiples, a la medida.

Info

Publication number
MX353478B
MX353478B MX2010001182A MX2010001182A MX353478B MX 353478 B MX353478 B MX 353478B MX 2010001182 A MX2010001182 A MX 2010001182A MX 2010001182 A MX2010001182 A MX 2010001182A MX 353478 B MX353478 B MX 353478B
Authority
MX
Mexico
Prior art keywords
primers
modified polynucleotides
primer
template
anneal
Prior art date
Application number
MX2010001182A
Other languages
English (en)
Other versions
MX2010001182A (es
Inventor
Tan Xuqiu
Original Assignee
Bp Corp North America Inc Star
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=40304880&utm_source=***_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX353478(B) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Bp Corp North America Inc Star filed Critical Bp Corp North America Inc Star
Publication of MX2010001182A publication Critical patent/MX2010001182A/es
Publication of MX353478B publication Critical patent/MX353478B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1024—In vivo mutagenesis using high mutation rate "mutator" host strains by inserting genetic material, e.g. encoding an error prone polymerase, disrupting a gene for mismatch repair
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La presente invención provee un novedoso método de producir una pluralidad de polinucleótidos modificados que tienen diferentes combinaciones de diversas mutaciones en múltiples sitios mediante un ensamblaje combinacional en sitios múltiples, a la medida, comprendiendo añadir al menos dos o al menos tres cebadores a un polinucleótido de plantilla de doble cadena en una sola mezcla de reacción, donde los cebadores no traslapan, y donde cada uno de los cebadores comprende al menos una mutación diferente de los demás cebadores, donde al menos un cebador es un cebador hacia adelante que puede templarse en una cadena menos de la plantilla y al menos un cebador es un cebador en reversa que puede templarse en una cadena mas de la plantilla, y someter la mezcla de reacción a una reacción de extensión de polimerasa para rendir una pluralidad de polinucleótidos modificados, extendidos a partir de los al menos tres cebadores. El método puede ser llevado a cabo sin emplear un paso de ligación antes de transformar los polinucleótidos modificados, extendidos en una célula. La pluralidad de polinucleótidos modificados, extendidos puede ser tratada con una enzima para destruir el polinucleótido de plantillas antes de transformarlo en la célula.
MX2010001182A 2007-07-31 2008-07-31 Ensamblaje combinacional de sitios multiples, a la medida. MX353478B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US95317107P 2007-07-31 2007-07-31
PCT/US2008/071771 WO2009018449A1 (en) 2007-07-31 2008-07-31 Tailored multi-site combinatorial assembly

Publications (2)

Publication Number Publication Date
MX2010001182A MX2010001182A (es) 2010-04-21
MX353478B true MX353478B (es) 2018-01-15

Family

ID=40304880

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2010001182A MX353478B (es) 2007-07-31 2008-07-31 Ensamblaje combinacional de sitios multiples, a la medida.

Country Status (16)

Country Link
US (1) US9476078B2 (es)
EP (2) EP3031919B1 (es)
JP (1) JP6014805B2 (es)
KR (1) KR101685712B1 (es)
CN (2) CN101821402B (es)
AU (1) AU2008282101A1 (es)
BR (1) BRPI0815046A2 (es)
CA (1) CA2693102C (es)
DK (1) DK3031919T3 (es)
EA (1) EA020657B1 (es)
ES (1) ES2701883T3 (es)
IL (1) IL202839A0 (es)
MX (1) MX353478B (es)
NZ (1) NZ582876A (es)
WO (1) WO2009018449A1 (es)
ZA (1) ZA201000152B (es)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8198062B2 (en) 2008-08-29 2012-06-12 Dsm Ip Assets B.V. Hydrolases, nucleic acids encoding them and methods for making and using them
EA021178B1 (ru) 2009-05-21 2015-04-30 Верениум Корпорейшн Фитазы, кодирующие их нуклеиновые кислоты и способы их получения и применения
EP2625269A2 (en) 2010-10-06 2013-08-14 BP Corporation North America Inc. Variant cbh i polypeptides with reduced product inhibition
WO2014070856A2 (en) 2012-11-02 2014-05-08 Bp Corporation North America Inc. Thermotolerant beta-glucosidase variants
GB201308828D0 (en) 2013-03-12 2013-07-03 Verenium Corp Phytase
GB201308843D0 (en) 2013-03-14 2013-07-03 Verenium Corp Phytase formulation
CN105408492A (zh) 2013-07-25 2016-03-16 巴斯夫酶有限责任公司 肌醇六磷酸酶
WO2018183612A1 (en) * 2017-03-29 2018-10-04 Editas Medicine, Inc. Nucleic acid mutagenesis methods
US11542487B2 (en) 2017-10-25 2023-01-03 Basf Se Beta-amylase enzymes
CN111788306A (zh) 2017-12-20 2020-10-16 巴斯夫欧洲公司 核酸酶以及用于制备和使用其的方法
EP3728620A4 (en) * 2017-12-20 2021-11-03 Basf Se GENE SITE SATURATION MUTAGENESIS (GSSM) PROCESS
CN112368375A (zh) 2018-04-26 2021-02-12 巴斯夫欧洲公司 脂肪酶
EP3802813A1 (en) 2018-05-29 2021-04-14 Basf Se Amylase enzymes
US20210317467A1 (en) 2018-09-07 2021-10-14 Basf Plant Science Copmany Gmbh Improved method for the production of high levels of pufa in plants
AU2019334671A1 (en) 2018-09-07 2021-03-25 Basf Plant Science Company Gmbh Improved method for the production of high levels of PUFA in plants
WO2020049155A1 (en) 2018-09-07 2020-03-12 Basf Plant Science Company Gmbh Improved method for the production of high levels of pufa in plants
WO2020185737A1 (en) 2019-03-11 2020-09-17 Basf Se Amylases and methods for making and using them
US20220220463A1 (en) 2019-04-23 2022-07-14 Basf Se Beta-amylase variants
JP2023508871A (ja) 2019-12-20 2023-03-06 ビーエーエスエフ ソシエタス・ヨーロピア テルペンの毒性の低減および微生物中での生成可能性の増大
EP4162061A1 (en) 2020-06-04 2023-04-12 Isobionics B.V. Synthetic santalene synthases
EP4015626A1 (en) 2020-12-18 2022-06-22 Isobionics B.V. Enzymes and methods for fermentative production of monoterpene esters
EP4381085A2 (en) 2021-08-02 2024-06-12 Basf Se Novel production of aroma compounds with ionylideneethane synthases

Family Cites Families (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5849482A (en) 1988-09-28 1998-12-15 Epoch Pharmaceuticals, Inc. Crosslinking oligonucleotides
ES2223040T3 (es) * 1991-04-10 2005-02-16 The Scripps Research Institute Bibliotecas de receptor heterodimerilo que usan fagemidos.
WO1993013216A1 (en) * 1991-12-24 1993-07-08 The President And Fellows Of Harvard College Site-directed mutagenesis of dna
US20030194807A1 (en) * 1992-11-02 2003-10-16 Roberto Crea Walk-through mutagenesis
CA2166079C (en) * 1993-06-25 2009-11-17 Peter Glazer Chemically modified oligonucleotide for site-directed mutagenesis
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6165793A (en) * 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6537776B1 (en) * 1999-06-14 2003-03-25 Diversa Corporation Synthetic ligation reassembly in directed evolution
US6764835B2 (en) * 1995-12-07 2004-07-20 Diversa Corporation Saturation mutageneis in directed evolution
US20020028443A1 (en) * 1999-09-27 2002-03-07 Jay M. Short Method of dna shuffling with polynucleotides produced by blocking or interrupting a synthesis or amplification process
US20040023327A1 (en) * 1995-12-07 2004-02-05 Short Jay M. End selection in directed evolution
US6358709B1 (en) * 1995-12-07 2002-03-19 Diversa Corporation End selection in directed evolution
US6740506B2 (en) * 1995-12-07 2004-05-25 Diversa Corporation End selection in directed evolution
US6562594B1 (en) * 1999-09-29 2003-05-13 Diversa Corporation Saturation mutagenesis in directed evolution
US6361974B1 (en) * 1995-12-07 2002-03-26 Diversa Corporation Exonuclease-mediated nucleic acid reassembly in directed evolution
US6238884B1 (en) * 1995-12-07 2001-05-29 Diversa Corporation End selection in directed evolution
US6713279B1 (en) * 1995-12-07 2004-03-30 Diversa Corporation Non-stochastic generation of genetic vaccines and enzymes
US6489145B1 (en) * 1996-07-09 2002-12-03 Diversa Corporation Method of DNA shuffling
US6171820B1 (en) 1995-12-07 2001-01-09 Diversa Corporation Saturation mutagenesis in directed evolution
US5830696A (en) * 1996-12-05 1998-11-03 Diversa Corporation Directed evolution of thermophilic enzymes
US5965408A (en) * 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
US6352842B1 (en) * 1995-12-07 2002-03-05 Diversa Corporation Exonucease-mediated gene assembly in directed evolution
US6939689B2 (en) * 1995-12-07 2005-09-06 Diversa Corporation Exonuclease-mediated nucleic acid reassembly in directed evolution
US6479258B1 (en) * 1995-12-07 2002-11-12 Diversa Corporation Non-stochastic generation of genetic vaccines
US5789166A (en) 1995-12-08 1998-08-04 Stratagene Circular site-directed mutagenesis
GB9701425D0 (en) * 1997-01-24 1997-03-12 Bioinvent Int Ab A method for in vitro molecular evolution of protein function
WO2000028015A1 (fr) * 1998-11-11 2000-05-18 Japan Science And Technology Corporation Methode de mutagenese
US6376191B1 (en) * 2000-03-22 2002-04-23 Mergen, Ltd. Microarray-based analysis of polynucleotide sequence variations
JP3859947B2 (ja) * 2000-08-04 2006-12-20 独立行政法人理化学研究所 突然変異導入方法
WO2002014490A2 (en) * 2000-08-11 2002-02-21 Genencor International, Inc. Bacillus transformation, transformants and mutant libraries
FR2813314B1 (fr) * 2000-08-25 2004-05-07 Biomethodes Procede de mutagenese dirigee massive
USPP13969P3 (en) 2001-04-05 2003-07-15 Her Majesty The Queen In Right Of Canada As Represented By The Minister Of Agriculture Shrub rose plant named ‘Morden Sunrise’
JP4473573B2 (ja) * 2001-07-26 2010-06-02 ストラタジーン カリフォルニア 多部位突然変異誘発
DE10148607A1 (de) * 2001-10-02 2003-04-10 Max Planck Gesellschaft Methoden zur DNA-Manipulation
US20030129709A1 (en) * 2001-11-02 2003-07-10 Olga Makarova Method for site-directed mutagenesis of nucleic acid molecules using a single primer
CA2468710A1 (en) * 2001-12-03 2003-06-12 Diversa Corporation Chromosomal saturation mutagenesis
AUPS263402A0 (en) * 2002-05-29 2002-06-20 Young, Lei Targeted amplification of template strand (TATS) technology, and its use in DNA mutagenesis, deletion & insertion
US20060134624A1 (en) * 2003-02-06 2006-06-22 Salerno John C Polymerase-based protocols for the introductions of deletions and insertions
US20060228786A1 (en) 2003-02-06 2006-10-12 Salerno John C Polymerase-based protocols for the introduction of deletions and insertions
US6878531B1 (en) * 2003-11-10 2005-04-12 Medical College Of Georgia Research Institute Method for multiple site-directed mutagenesis
US20060288786A1 (en) * 2005-03-18 2006-12-28 Flores Andres H L Ultrasonically coupled scanning probe microscope
US20060223066A1 (en) * 2005-03-29 2006-10-05 Applera Corporation Methods for normalizing and for identifying small nucleic acids
WO2007002890A2 (en) * 2005-06-28 2007-01-04 Agencourt Personal Genomics Corporation Methods of producing and sequencing modified polynucleotides

Also Published As

Publication number Publication date
KR20100045494A (ko) 2010-05-03
DK3031919T3 (en) 2019-01-07
JP6014805B2 (ja) 2016-10-26
EP2183378A4 (en) 2010-09-22
BRPI0815046A2 (pt) 2014-10-14
KR101685712B1 (ko) 2016-12-12
MX2010001182A (es) 2010-04-21
NZ582876A (en) 2012-07-27
US9476078B2 (en) 2016-10-25
EP3031919B1 (en) 2018-09-19
CN104673784A (zh) 2015-06-03
CA2693102C (en) 2020-03-10
ZA201000152B (en) 2011-06-29
EA020657B1 (ru) 2014-12-30
EA201070206A1 (ru) 2010-06-30
ES2701883T3 (es) 2019-02-26
AU2008282101A1 (en) 2009-02-05
EP2183378A1 (en) 2010-05-12
CN101821402B (zh) 2015-03-04
CA2693102A1 (en) 2009-02-05
EP3031919A1 (en) 2016-06-15
WO2009018449A1 (en) 2009-02-05
IL202839A0 (en) 2010-06-30
CN101821402A (zh) 2010-09-01
JP2010535034A (ja) 2010-11-18
US20100216192A1 (en) 2010-08-26

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Owner name: BP CORPORATION NORTH AMERICA INC.*

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