MX353478B - Ensamblaje combinacional de sitios multiples, a la medida. - Google Patents
Ensamblaje combinacional de sitios multiples, a la medida.Info
- Publication number
- MX353478B MX353478B MX2010001182A MX2010001182A MX353478B MX 353478 B MX353478 B MX 353478B MX 2010001182 A MX2010001182 A MX 2010001182A MX 2010001182 A MX2010001182 A MX 2010001182A MX 353478 B MX353478 B MX 353478B
- Authority
- MX
- Mexico
- Prior art keywords
- primers
- modified polynucleotides
- primer
- template
- anneal
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
- C12N15/1024—In vivo mutagenesis using high mutation rate "mutator" host strains by inserting genetic material, e.g. encoding an error prone polymerase, disrupting a gene for mismatch repair
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/185—Escherichia
- C12R2001/19—Escherichia coli
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
La presente invención provee un novedoso método de producir una pluralidad de polinucleótidos modificados que tienen diferentes combinaciones de diversas mutaciones en múltiples sitios mediante un ensamblaje combinacional en sitios múltiples, a la medida, comprendiendo añadir al menos dos o al menos tres cebadores a un polinucleótido de plantilla de doble cadena en una sola mezcla de reacción, donde los cebadores no traslapan, y donde cada uno de los cebadores comprende al menos una mutación diferente de los demás cebadores, donde al menos un cebador es un cebador hacia adelante que puede templarse en una cadena menos de la plantilla y al menos un cebador es un cebador en reversa que puede templarse en una cadena mas de la plantilla, y someter la mezcla de reacción a una reacción de extensión de polimerasa para rendir una pluralidad de polinucleótidos modificados, extendidos a partir de los al menos tres cebadores. El método puede ser llevado a cabo sin emplear un paso de ligación antes de transformar los polinucleótidos modificados, extendidos en una célula. La pluralidad de polinucleótidos modificados, extendidos puede ser tratada con una enzima para destruir el polinucleótido de plantillas antes de transformarlo en la célula.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US95317107P | 2007-07-31 | 2007-07-31 | |
PCT/US2008/071771 WO2009018449A1 (en) | 2007-07-31 | 2008-07-31 | Tailored multi-site combinatorial assembly |
Publications (2)
Publication Number | Publication Date |
---|---|
MX2010001182A MX2010001182A (es) | 2010-04-21 |
MX353478B true MX353478B (es) | 2018-01-15 |
Family
ID=40304880
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2010001182A MX353478B (es) | 2007-07-31 | 2008-07-31 | Ensamblaje combinacional de sitios multiples, a la medida. |
Country Status (16)
Country | Link |
---|---|
US (1) | US9476078B2 (es) |
EP (2) | EP3031919B1 (es) |
JP (1) | JP6014805B2 (es) |
KR (1) | KR101685712B1 (es) |
CN (2) | CN101821402B (es) |
AU (1) | AU2008282101A1 (es) |
BR (1) | BRPI0815046A2 (es) |
CA (1) | CA2693102C (es) |
DK (1) | DK3031919T3 (es) |
EA (1) | EA020657B1 (es) |
ES (1) | ES2701883T3 (es) |
IL (1) | IL202839A0 (es) |
MX (1) | MX353478B (es) |
NZ (1) | NZ582876A (es) |
WO (1) | WO2009018449A1 (es) |
ZA (1) | ZA201000152B (es) |
Families Citing this family (22)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8198062B2 (en) | 2008-08-29 | 2012-06-12 | Dsm Ip Assets B.V. | Hydrolases, nucleic acids encoding them and methods for making and using them |
EA021178B1 (ru) | 2009-05-21 | 2015-04-30 | Верениум Корпорейшн | Фитазы, кодирующие их нуклеиновые кислоты и способы их получения и применения |
EP2625269A2 (en) | 2010-10-06 | 2013-08-14 | BP Corporation North America Inc. | Variant cbh i polypeptides with reduced product inhibition |
WO2014070856A2 (en) | 2012-11-02 | 2014-05-08 | Bp Corporation North America Inc. | Thermotolerant beta-glucosidase variants |
GB201308828D0 (en) | 2013-03-12 | 2013-07-03 | Verenium Corp | Phytase |
GB201308843D0 (en) | 2013-03-14 | 2013-07-03 | Verenium Corp | Phytase formulation |
CN105408492A (zh) | 2013-07-25 | 2016-03-16 | 巴斯夫酶有限责任公司 | 肌醇六磷酸酶 |
WO2018183612A1 (en) * | 2017-03-29 | 2018-10-04 | Editas Medicine, Inc. | Nucleic acid mutagenesis methods |
US11542487B2 (en) | 2017-10-25 | 2023-01-03 | Basf Se | Beta-amylase enzymes |
CN111788306A (zh) | 2017-12-20 | 2020-10-16 | 巴斯夫欧洲公司 | 核酸酶以及用于制备和使用其的方法 |
EP3728620A4 (en) * | 2017-12-20 | 2021-11-03 | Basf Se | GENE SITE SATURATION MUTAGENESIS (GSSM) PROCESS |
CN112368375A (zh) | 2018-04-26 | 2021-02-12 | 巴斯夫欧洲公司 | 脂肪酶 |
EP3802813A1 (en) | 2018-05-29 | 2021-04-14 | Basf Se | Amylase enzymes |
US20210317467A1 (en) | 2018-09-07 | 2021-10-14 | Basf Plant Science Copmany Gmbh | Improved method for the production of high levels of pufa in plants |
AU2019334671A1 (en) | 2018-09-07 | 2021-03-25 | Basf Plant Science Company Gmbh | Improved method for the production of high levels of PUFA in plants |
WO2020049155A1 (en) | 2018-09-07 | 2020-03-12 | Basf Plant Science Company Gmbh | Improved method for the production of high levels of pufa in plants |
WO2020185737A1 (en) | 2019-03-11 | 2020-09-17 | Basf Se | Amylases and methods for making and using them |
US20220220463A1 (en) | 2019-04-23 | 2022-07-14 | Basf Se | Beta-amylase variants |
JP2023508871A (ja) | 2019-12-20 | 2023-03-06 | ビーエーエスエフ ソシエタス・ヨーロピア | テルペンの毒性の低減および微生物中での生成可能性の増大 |
EP4162061A1 (en) | 2020-06-04 | 2023-04-12 | Isobionics B.V. | Synthetic santalene synthases |
EP4015626A1 (en) | 2020-12-18 | 2022-06-22 | Isobionics B.V. | Enzymes and methods for fermentative production of monoterpene esters |
EP4381085A2 (en) | 2021-08-02 | 2024-06-12 | Basf Se | Novel production of aroma compounds with ionylideneethane synthases |
Family Cites Families (45)
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US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
US5849482A (en) | 1988-09-28 | 1998-12-15 | Epoch Pharmaceuticals, Inc. | Crosslinking oligonucleotides |
ES2223040T3 (es) * | 1991-04-10 | 2005-02-16 | The Scripps Research Institute | Bibliotecas de receptor heterodimerilo que usan fagemidos. |
WO1993013216A1 (en) * | 1991-12-24 | 1993-07-08 | The President And Fellows Of Harvard College | Site-directed mutagenesis of dna |
US20030194807A1 (en) * | 1992-11-02 | 2003-10-16 | Roberto Crea | Walk-through mutagenesis |
CA2166079C (en) * | 1993-06-25 | 2009-11-17 | Peter Glazer | Chemically modified oligonucleotide for site-directed mutagenesis |
US6335160B1 (en) | 1995-02-17 | 2002-01-01 | Maxygen, Inc. | Methods and compositions for polypeptide engineering |
US6165793A (en) * | 1996-03-25 | 2000-12-26 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
US6537776B1 (en) * | 1999-06-14 | 2003-03-25 | Diversa Corporation | Synthetic ligation reassembly in directed evolution |
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US20020028443A1 (en) * | 1999-09-27 | 2002-03-07 | Jay M. Short | Method of dna shuffling with polynucleotides produced by blocking or interrupting a synthesis or amplification process |
US20040023327A1 (en) * | 1995-12-07 | 2004-02-05 | Short Jay M. | End selection in directed evolution |
US6358709B1 (en) * | 1995-12-07 | 2002-03-19 | Diversa Corporation | End selection in directed evolution |
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US6238884B1 (en) * | 1995-12-07 | 2001-05-29 | Diversa Corporation | End selection in directed evolution |
US6713279B1 (en) * | 1995-12-07 | 2004-03-30 | Diversa Corporation | Non-stochastic generation of genetic vaccines and enzymes |
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US5965408A (en) * | 1996-07-09 | 1999-10-12 | Diversa Corporation | Method of DNA reassembly by interrupting synthesis |
US6352842B1 (en) * | 1995-12-07 | 2002-03-05 | Diversa Corporation | Exonucease-mediated gene assembly in directed evolution |
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-
2008
- 2008-07-31 BR BRPI0815046-0A2A patent/BRPI0815046A2/pt not_active Application Discontinuation
- 2008-07-31 KR KR1020107003986A patent/KR101685712B1/ko active IP Right Grant
- 2008-07-31 CN CN200880101253.0A patent/CN101821402B/zh not_active Expired - Fee Related
- 2008-07-31 AU AU2008282101A patent/AU2008282101A1/en not_active Abandoned
- 2008-07-31 CA CA2693102A patent/CA2693102C/en active Active
- 2008-07-31 NZ NZ582876A patent/NZ582876A/en not_active IP Right Cessation
- 2008-07-31 JP JP2010520191A patent/JP6014805B2/ja not_active Expired - Fee Related
- 2008-07-31 EP EP16154206.3A patent/EP3031919B1/en active Active
- 2008-07-31 CN CN201510050333.XA patent/CN104673784A/zh active Pending
- 2008-07-31 US US12/671,231 patent/US9476078B2/en active Active
- 2008-07-31 EP EP08782567A patent/EP2183378A4/en not_active Ceased
- 2008-07-31 MX MX2010001182A patent/MX353478B/es active IP Right Grant
- 2008-07-31 EA EA201070206A patent/EA020657B1/ru not_active IP Right Cessation
- 2008-07-31 ES ES16154206T patent/ES2701883T3/es active Active
- 2008-07-31 DK DK16154206.3T patent/DK3031919T3/en active
- 2008-07-31 WO PCT/US2008/071771 patent/WO2009018449A1/en active Application Filing
-
2009
- 2009-12-20 IL IL202839A patent/IL202839A0/en unknown
-
2010
- 2010-01-08 ZA ZA2010/00152A patent/ZA201000152B/en unknown
Also Published As
Publication number | Publication date |
---|---|
KR20100045494A (ko) | 2010-05-03 |
DK3031919T3 (en) | 2019-01-07 |
JP6014805B2 (ja) | 2016-10-26 |
EP2183378A4 (en) | 2010-09-22 |
BRPI0815046A2 (pt) | 2014-10-14 |
KR101685712B1 (ko) | 2016-12-12 |
MX2010001182A (es) | 2010-04-21 |
NZ582876A (en) | 2012-07-27 |
US9476078B2 (en) | 2016-10-25 |
EP3031919B1 (en) | 2018-09-19 |
CN104673784A (zh) | 2015-06-03 |
CA2693102C (en) | 2020-03-10 |
ZA201000152B (en) | 2011-06-29 |
EA020657B1 (ru) | 2014-12-30 |
EA201070206A1 (ru) | 2010-06-30 |
ES2701883T3 (es) | 2019-02-26 |
AU2008282101A1 (en) | 2009-02-05 |
EP2183378A1 (en) | 2010-05-12 |
CN101821402B (zh) | 2015-03-04 |
CA2693102A1 (en) | 2009-02-05 |
EP3031919A1 (en) | 2016-06-15 |
WO2009018449A1 (en) | 2009-02-05 |
IL202839A0 (en) | 2010-06-30 |
CN101821402A (zh) | 2010-09-01 |
JP2010535034A (ja) | 2010-11-18 |
US20100216192A1 (en) | 2010-08-26 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
GB | Transfer or rights |
Owner name: BP CORPORATION NORTH AMERICA INC.* |
|
FG | Grant or registration |