WO2011104695A3 - Detection of kras mutation in exon 2 by allele specific real time quantitative pcr (as-qpcr) - Google Patents
Detection of kras mutation in exon 2 by allele specific real time quantitative pcr (as-qpcr) Download PDFInfo
- Publication number
- WO2011104695A3 WO2011104695A3 PCT/IB2011/050819 IB2011050819W WO2011104695A3 WO 2011104695 A3 WO2011104695 A3 WO 2011104695A3 IB 2011050819 W IB2011050819 W IB 2011050819W WO 2011104695 A3 WO2011104695 A3 WO 2011104695A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- allele
- qpcr
- exon
- detection
- real time
- Prior art date
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/106—Pharmacogenomics, i.e. genetic variability in individual responses to drugs and drug metabolism
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Pathology (AREA)
- Hospice & Palliative Care (AREA)
- Oncology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
A method for conducting an allele specific polymerase chain reaction which comprises subjecting DNA which may contain either or both of first and second alleles to a polymerase chain reaction utilizing a primer pair in which one of the primers is complementary to said first allele, but which primer forms a mismatch with said second allele at the 3'-terminal nucleotide of the primer, and utilizing a DNA polymerase wherein said first allele is specifically amplified but no amplification of said second allele occurs.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
IB2010050843 | 2010-02-26 | ||
IBPCT/IB2010/050843 | 2010-02-26 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2011104695A2 WO2011104695A2 (en) | 2011-09-01 |
WO2011104695A3 true WO2011104695A3 (en) | 2012-03-01 |
Family
ID=44507312
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/IB2011/050819 WO2011104695A2 (en) | 2010-02-26 | 2011-02-25 | Detection of kras mutation in exon 2 by allele specific real time quantitative pcr (as-qpcr) |
Country Status (1)
Country | Link |
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WO (1) | WO2011104695A2 (en) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102367478B (en) * | 2011-10-25 | 2013-07-31 | 浙江大学 | ARMS-qPCR (Allele Refractory Mutation System-quantitative Polymerase Chain Reaction) detection kit for KRAS (Kirsten Rat Sarcoma Viral Oncogene Homolog) gene mutation subtype and detection method |
CN103930571A (en) * | 2011-11-17 | 2014-07-16 | 瑞尼克斯有限公司 | System and methods for selective molecular analysis |
CN102796816B (en) * | 2012-07-06 | 2014-04-16 | 陕西北美基因股份有限公司 | Method for performing in-vitro accurate test on KRAS gene mutation |
WO2014052613A2 (en) * | 2012-09-26 | 2014-04-03 | Insight Genetics, Inc. | Methods and compositions relating to next generation sequencing for genetic testing in alk related cancers |
CN104805207A (en) * | 2015-04-29 | 2015-07-29 | 苏州工业园区为真生物医药科技有限公司 | Kit for detecting kras gene mutation, and detection method of kit |
JP6754202B2 (en) * | 2016-03-15 | 2020-09-09 | アークレイ株式会社 | K-ras gene amplification forward primer set, K-ras gene amplification kit, K-ras gene amplification method, polymorphism analysis method, and drug efficacy determination method |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5639611A (en) * | 1988-12-12 | 1997-06-17 | City Of Hope | Allele specific polymerase chain reaction |
WO2000056916A2 (en) * | 1999-03-18 | 2000-09-28 | Exiqon A/S | Detection of mutations in genes by specific lna primers |
EP1247815A2 (en) * | 2001-03-25 | 2002-10-09 | Exiqon A/S | Modified oligonucleotides and uses thereof |
WO2003072814A2 (en) * | 2002-02-26 | 2003-09-04 | Roche Diagnostics Gmbh | Improved method for allele-specific pcr |
WO2008070370A2 (en) * | 2006-11-02 | 2008-06-12 | University Of Utah Research Foundation | Oligonucleotides for use in allele-specific pcr |
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2011
- 2011-02-25 WO PCT/IB2011/050819 patent/WO2011104695A2/en active Application Filing
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5639611A (en) * | 1988-12-12 | 1997-06-17 | City Of Hope | Allele specific polymerase chain reaction |
WO2000056916A2 (en) * | 1999-03-18 | 2000-09-28 | Exiqon A/S | Detection of mutations in genes by specific lna primers |
EP1247815A2 (en) * | 2001-03-25 | 2002-10-09 | Exiqon A/S | Modified oligonucleotides and uses thereof |
WO2003072814A2 (en) * | 2002-02-26 | 2003-09-04 | Roche Diagnostics Gmbh | Improved method for allele-specific pcr |
WO2008070370A2 (en) * | 2006-11-02 | 2008-06-12 | University Of Utah Research Foundation | Oligonucleotides for use in allele-specific pcr |
Non-Patent Citations (3)
Title |
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JOHNSON M P ET AL: "LOCKED NUCLEIC ACID (LNA) SINGLE NUCLEOTIDE POLYMORPHISM (SNP) GENOTYPE ANALYSIS AND VALIDATION USING REAL-TIME PCR", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 32, no. 6, 26 March 2004 (2004-03-26), pages E55 - 01, XP009069933, ISSN: 0305-1048 * |
LATORRA D ET AL: "ENHANCED ALLELE-SPECIFIC PCR DISCRIMINATION IN SNP GENOTYPING USING 3' LOCKED NUCLEIC ACID (LNA) PRIMERS", HUMAN MUTATION, JOHN WILEY & SONS, INC, US, vol. 22, no. 1, 1 July 2003 (2003-07-01), pages 79 - 85, XP009043978, ISSN: 1059-7794, DOI: 10.1002/HUMU.10228 * |
STRAND ET AL: "Real-time detection and quantification of mitochondrial mutations with oligonucleotide primers containing locked nucleic acid", CLINICA CHIMICA ACTA, ELSEVIER BV, AMSTERDAM, NL, vol. 390, no. 1-2, 16 January 2008 (2008-01-16), pages 126 - 133, XP022499213, ISSN: 0009-8981, DOI: 10.1016/J.CCA.2008.01.010 * |
Also Published As
Publication number | Publication date |
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WO2011104695A2 (en) | 2011-09-01 |
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