MX346663B - Lineas de celulas de bajo nivel de fucosa y usos de las mismas. - Google Patents
Lineas de celulas de bajo nivel de fucosa y usos de las mismas.Info
- Publication number
- MX346663B MX346663B MX2013010210A MX2013010210A MX346663B MX 346663 B MX346663 B MX 346663B MX 2013010210 A MX2013010210 A MX 2013010210A MX 2013010210 A MX2013010210 A MX 2013010210A MX 346663 B MX346663 B MX 346663B
- Authority
- MX
- Mexico
- Prior art keywords
- cells
- fucose
- population
- recombinant proteins
- binding agent
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/2863—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for growth factors, growth regulators
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
- C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
- C12Y101/01271—GDP-L-fucose synthase (1.1.1.271)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/01—Hexosyltransferases (2.4.1)
- C12Y204/01068—Glycoprotein 6-alpha-L-fucosyltransferase (2.4.1.68), i.e. FUT8
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y402/00—Carbon-oxygen lyases (4.2)
- C12Y402/01—Hydro-lyases (4.2.1)
- C12Y402/01047—GDP-mannose 4,6-dehydratase (4.2.1.47), i.e. GMD
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
- C07K2317/41—Glycosylation, sialylation, or fucosylation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
La presente invención se refiere a un método de selección de células que tienen cero nivel de fucosa, útiles como células hospederas para la expresión de proteínas recombinantes; el método comprende: (d) introducir mutaciones genéticas en una población de células CHO poniendo en contacto las células con metotrexato (MTX), (e) poner en contacto la población de células CHO que comprenden células mutadas con un agente que se une a la fucosa no tóxico por una cantidad de tiempo que permita la unión del agente que se une a la fucosa a una porción de fucosa sobre la membrana de una célula de la población de células, en donde la cantidad de tiempo no permita la destrucción de las células; y (f) agotar de la población de células que comprenden células mutadas, una subpoblación de células que se unen al agente que se une a la fucosa, seleccionando de esta manera células útiles como células hospederas para la expresión de proteínas recombinantes, las células seleccionadas teniendo cero contenido de fucosa; se describen también células y líneas de células útiles como células hospederas para la expresión de proteínas recombinantes.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
IL211583A IL211583A0 (en) | 2011-03-06 | 2011-03-06 | Low fucose cell lines and uses thereof |
IL217216A IL217216A0 (en) | 2011-12-27 | 2011-12-27 | Low fucose cell lins and uses thereof |
PCT/IL2012/000109 WO2012120500A2 (en) | 2011-03-06 | 2012-03-05 | Low fucose cell lines and uses thereof |
Publications (2)
Publication Number | Publication Date |
---|---|
MX2013010210A MX2013010210A (es) | 2013-09-26 |
MX346663B true MX346663B (es) | 2017-03-27 |
Family
ID=45952581
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2013010210A MX346663B (es) | 2011-03-06 | 2012-03-05 | Lineas de celulas de bajo nivel de fucosa y usos de las mismas. |
Country Status (14)
Country | Link |
---|---|
US (1) | US9574003B2 (es) |
EP (1) | EP2683821B1 (es) |
JP (2) | JP2014509850A (es) |
CN (2) | CN103597073B (es) |
AU (1) | AU2012226398B9 (es) |
BR (1) | BR112013022832A2 (es) |
CA (1) | CA2829110C (es) |
EA (1) | EA032513B1 (es) |
ES (1) | ES2687771T3 (es) |
IL (1) | IL228184B (es) |
MX (1) | MX346663B (es) |
SG (1) | SG192632A1 (es) |
WO (1) | WO2012120500A2 (es) |
ZA (1) | ZA201306336B (es) |
Families Citing this family (38)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8911964B2 (en) | 2006-09-13 | 2014-12-16 | Abbvie Inc. | Fed-batch method of making human anti-TNF-alpha antibody |
BRPI0716762A2 (pt) | 2006-09-13 | 2013-09-24 | Abbott Lab | melhorias da cultura celular |
CA2738499A1 (en) | 2008-10-20 | 2010-04-29 | Abbott Laboratories | Viral inactivation during purification of antibodies |
EP2921501A1 (en) | 2008-10-20 | 2015-09-23 | Abbvie Inc. | Isolation and purification of antibodies using Protein A affinity chromatography |
KR101441437B1 (ko) | 2009-06-02 | 2014-09-25 | 리제너론 파마슈티칼스 인코포레이티드 | 푸코실화-결핍 세포 |
TW201302793A (zh) | 2010-09-03 | 2013-01-16 | Glaxo Group Ltd | 新穎之抗原結合蛋白 |
EP2702077A2 (en) | 2011-04-27 | 2014-03-05 | AbbVie Inc. | Methods for controlling the galactosylation profile of recombinantly-expressed proteins |
US9334319B2 (en) | 2012-04-20 | 2016-05-10 | Abbvie Inc. | Low acidic species compositions |
US9067990B2 (en) | 2013-03-14 | 2015-06-30 | Abbvie, Inc. | Protein purification using displacement chromatography |
US9181572B2 (en) | 2012-04-20 | 2015-11-10 | Abbvie, Inc. | Methods to modulate lysine variant distribution |
US9249182B2 (en) | 2012-05-24 | 2016-02-02 | Abbvie, Inc. | Purification of antibodies using hydrophobic interaction chromatography |
US9512214B2 (en) | 2012-09-02 | 2016-12-06 | Abbvie, Inc. | Methods to control protein heterogeneity |
KR20150043523A (ko) | 2012-09-02 | 2015-04-22 | 애브비 인코포레이티드 | 단백질 불균일성의 제어 방법 |
US20150322119A1 (en) | 2012-12-03 | 2015-11-12 | Bristol-Myers Squibb Company | Enhancing anti-cancer activity of immunomodulatory fc fusion proteins |
SG11201507230PA (en) | 2013-03-12 | 2015-10-29 | Abbvie Inc | Human antibodies that bind human tnf-alpha and methods of preparing the same |
US9499614B2 (en) | 2013-03-14 | 2016-11-22 | Abbvie Inc. | Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosaccharides |
US8921526B2 (en) | 2013-03-14 | 2014-12-30 | Abbvie, Inc. | Mutated anti-TNFα antibodies and methods of their use |
US9017687B1 (en) | 2013-10-18 | 2015-04-28 | Abbvie, Inc. | Low acidic species compositions and methods for producing and using the same using displacement chromatography |
US9598667B2 (en) | 2013-10-04 | 2017-03-21 | Abbvie Inc. | Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins |
US9181337B2 (en) | 2013-10-18 | 2015-11-10 | Abbvie, Inc. | Modulated lysine variant species compositions and methods for producing and using the same |
US8946395B1 (en) | 2013-10-18 | 2015-02-03 | Abbvie Inc. | Purification of proteins using hydrophobic interaction chromatography |
US9085618B2 (en) | 2013-10-18 | 2015-07-21 | Abbvie, Inc. | Low acidic species compositions and methods for producing and using the same |
US20150139988A1 (en) | 2013-11-15 | 2015-05-21 | Abbvie, Inc. | Glycoengineered binding protein compositions |
BR112016013109B1 (pt) * | 2013-12-09 | 2021-05-11 | Allakos Inc. | anticorpo anti-siglec-8, ácido nucleico, vetor, célula hospedeira, composição farmancêutica, seu método de produção e seus usos |
EP3122353B1 (en) | 2014-03-27 | 2020-10-28 | Vanderbilt University | Substituted indole mcl-1 inhibitors |
WO2016075662A2 (en) * | 2014-11-15 | 2016-05-19 | Zumutor Biologics, Inc. | Dna-binding domain, non-fucosylated and partially fucosylated proteins, and methods thereof |
ES2784503T3 (es) | 2014-12-01 | 2020-09-28 | Amgen Inc | Procedimiento para manipular el nivel de contenido de glicano de una glicoproteína |
KR20180068967A (ko) | 2015-11-02 | 2018-06-22 | 제넨테크, 인크. | 단백질의 푸코실화 및 탈푸코실화 형태를 제조하는 방법 |
US11186858B1 (en) | 2016-03-15 | 2021-11-30 | Fresenius Kabi Deutschland Gmbh | Methods for increasing biosimilarity |
JP2020518638A (ja) | 2017-05-05 | 2020-06-25 | アラコス インコーポレイテッド | アレルギー性眼疾患を処置するための方法および組成物 |
KR102596303B1 (ko) * | 2018-08-29 | 2023-10-30 | 유나이티드 바이오파마, 인크. | 비푸코실화된 항체 및 그의 제조법 |
AU2018438163A1 (en) * | 2018-08-29 | 2021-04-01 | United Biopharma Inc | Afucosylated antibodies and manufacture thereof |
WO2020090747A1 (ja) | 2018-10-29 | 2020-05-07 | 株式会社免疫生物研究所 | 抗hiv抗体及びその製造方法 |
MA55282A (fr) * | 2019-03-14 | 2022-01-19 | Janssen Biotech Inc | Procédés de fabrication pour la production de compositions d'anticorps anti-tnf |
JP2022526881A (ja) * | 2019-03-14 | 2022-05-27 | ヤンセン バイオテツク,インコーポレーテツド | 抗tnf抗体組成物を産生するための方法 |
US20220153829A1 (en) * | 2019-03-14 | 2022-05-19 | Janssen Biotech, Inc. | Methods for Producing Anti-TNF Antibody Compositions |
CA3177604A1 (en) * | 2020-04-17 | 2021-10-21 | Janssen Biotech, Inc. | Biosynthetic glycoprotein populations |
KR20230087539A (ko) * | 2020-10-15 | 2023-06-16 | 암젠 인크 | 항체 생산 방법에서의 상대적인 비결합 글리칸 |
Family Cites Families (32)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
NL154600B (nl) | 1971-02-10 | 1977-09-15 | Organon Nv | Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen. |
NL154598B (nl) | 1970-11-10 | 1977-09-15 | Organon Nv | Werkwijze voor het aantonen en bepalen van laagmoleculire verbindingen en van eiwitten die deze verbindingen specifiek kunnen binden, alsmede testverpakking. |
NL154599B (nl) | 1970-12-28 | 1977-09-15 | Organon Nv | Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen, alsmede testverpakking. |
US3901654A (en) | 1971-06-21 | 1975-08-26 | Biological Developments | Receptor assays of biologically active compounds employing biologically specific receptors |
US3853987A (en) | 1971-09-01 | 1974-12-10 | W Dreyer | Immunological reagent and radioimmuno assay |
US3867517A (en) | 1971-12-21 | 1975-02-18 | Abbott Lab | Direct radioimmunoassay for antigens and their antibodies |
NL171930C (nl) | 1972-05-11 | 1983-06-01 | Akzo Nv | Werkwijze voor het aantonen en bepalen van haptenen, alsmede testverpakkingen. |
US3850578A (en) | 1973-03-12 | 1974-11-26 | H Mcconnell | Process for assaying for biologically active molecules |
US3935074A (en) | 1973-12-17 | 1976-01-27 | Syva Company | Antibody steric hindrance immunoassay with two antibodies |
US3996345A (en) | 1974-08-12 | 1976-12-07 | Syva Company | Fluorescence quenching with immunological pairs in immunoassays |
US4034074A (en) | 1974-09-19 | 1977-07-05 | The Board Of Trustees Of Leland Stanford Junior University | Universal reagent 2-site immunoradiometric assay using labelled anti (IgG) |
US3984533A (en) | 1975-11-13 | 1976-10-05 | General Electric Company | Electrophoretic method of detecting antigen-antibody reaction |
US4098876A (en) | 1976-10-26 | 1978-07-04 | Corning Glass Works | Reverse sandwich immunoassay |
US4879219A (en) | 1980-09-19 | 1989-11-07 | General Hospital Corporation | Immunoassay utilizing monoclonal high affinity IgM antibodies |
US5011771A (en) | 1984-04-12 | 1991-04-30 | The General Hospital Corporation | Multiepitopic immunometric assay |
US4666828A (en) | 1984-08-15 | 1987-05-19 | The General Hospital Corporation | Test for Huntington's disease |
US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US4801531A (en) | 1985-04-17 | 1989-01-31 | Biotechnology Research Partners, Ltd. | Apo AI/CIII genomic polymorphisms predictive of atherosclerosis |
US5272057A (en) | 1988-10-14 | 1993-12-21 | Georgetown University | Method of detecting a predisposition to cancer by the use of restriction fragment length polymorphism of the gene for human poly (ADP-ribose) polymerase |
US5192659A (en) | 1989-08-25 | 1993-03-09 | Genetype Ag | Intron sequence analysis method for detection of adjacent and remote locus alleles as haplotypes |
US5281521A (en) | 1992-07-20 | 1994-01-25 | The Trustees Of The University Of Pennsylvania | Modified avidin-biotin technique |
EP1060242A4 (en) * | 1998-01-23 | 2003-09-17 | Imclone Systems Inc | PURIFIED POPULATIONS OF STEM CELLS |
HU231090B1 (hu) * | 2000-10-06 | 2020-07-28 | Kyowa Kirin Co., Ltd. | Antitest-kompozíciót termelő sejt |
US6569268B1 (en) * | 2000-10-16 | 2003-05-27 | Teck Cominco Metals Ltd. | Process and alloy for decorative galvanizing of steel |
JP4437015B2 (ja) * | 2003-05-21 | 2010-03-24 | タカラバイオ株式会社 | 肝幹細胞の分離方法 |
CN101023172A (zh) * | 2004-08-05 | 2007-08-22 | 协和发酵工业株式会社 | 糖蛋白质组合物的制造方法 |
WO2006089232A2 (en) * | 2005-02-18 | 2006-08-24 | Medarex, Inc. | Monoclonal antibodies against cd30 lacking fucosyl residues |
SG187521A1 (en) * | 2007-03-07 | 2013-02-28 | Glycofi Inc | Production of glycoproteins with modified fucosylation |
CN102105490B (zh) * | 2008-07-22 | 2015-08-05 | J-制油株式会社 | L-岩藻糖α1→6特异性凝集素 |
JP2012503656A (ja) * | 2008-09-26 | 2012-02-09 | エウレカ セラピューティクス,インコーポレイテッド | 変異体グリコシル化パターンを有する細胞株およびタンパク質 |
KR101441437B1 (ko) | 2009-06-02 | 2014-09-25 | 리제너론 파마슈티칼스 인코포레이티드 | 푸코실화-결핍 세포 |
EP2950098A1 (en) * | 2009-07-14 | 2015-12-02 | National Institute of Advanced Industrial Science and Technology | Glycan markers as measure of disease state of hepatic diseases |
-
2012
- 2012-03-05 US US14/003,767 patent/US9574003B2/en active Active
- 2012-03-05 WO PCT/IL2012/000109 patent/WO2012120500A2/en active Application Filing
- 2012-03-05 BR BR112013022832A patent/BR112013022832A2/pt not_active Application Discontinuation
- 2012-03-05 CN CN201280021537.5A patent/CN103597073B/zh active Active
- 2012-03-05 CN CN201910129277.7A patent/CN109797138A/zh active Pending
- 2012-03-05 CA CA2829110A patent/CA2829110C/en active Active
- 2012-03-05 SG SG2013059639A patent/SG192632A1/en unknown
- 2012-03-05 EP EP12713786.7A patent/EP2683821B1/en active Active
- 2012-03-05 AU AU2012226398A patent/AU2012226398B9/en active Active
- 2012-03-05 EA EA201391282A patent/EA032513B1/ru not_active IP Right Cessation
- 2012-03-05 ES ES12713786.7T patent/ES2687771T3/es active Active
- 2012-03-05 MX MX2013010210A patent/MX346663B/es active IP Right Grant
- 2012-03-05 JP JP2013557220A patent/JP2014509850A/ja active Pending
-
2013
- 2013-08-22 ZA ZA2013/06336A patent/ZA201306336B/en unknown
- 2013-08-29 IL IL228184A patent/IL228184B/en active IP Right Grant
-
2016
- 2016-12-22 JP JP2016249691A patent/JP6353023B2/ja active Active
Also Published As
Publication number | Publication date |
---|---|
WO2012120500A2 (en) | 2012-09-13 |
JP6353023B2 (ja) | 2018-07-04 |
CN109797138A (zh) | 2019-05-24 |
BR112013022832A2 (pt) | 2016-11-22 |
AU2012226398A1 (en) | 2013-09-12 |
EP2683821B1 (en) | 2018-07-25 |
CA2829110A1 (en) | 2012-09-13 |
IL228184B (en) | 2018-12-31 |
JP2014509850A (ja) | 2014-04-24 |
EA032513B1 (ru) | 2019-06-28 |
CN103597073A (zh) | 2014-02-19 |
ZA201306336B (en) | 2014-10-29 |
EP2683821A2 (en) | 2014-01-15 |
SG192632A1 (en) | 2013-09-30 |
AU2012226398B9 (en) | 2017-04-13 |
JP2017079775A (ja) | 2017-05-18 |
WO2012120500A3 (en) | 2012-11-15 |
ES2687771T3 (es) | 2018-10-29 |
AU2012226398B2 (en) | 2017-03-23 |
MX2013010210A (es) | 2013-09-26 |
CN103597073B (zh) | 2019-06-07 |
EA201391282A1 (ru) | 2014-04-30 |
US9574003B2 (en) | 2017-02-21 |
US20140005368A1 (en) | 2014-01-02 |
CA2829110C (en) | 2019-01-15 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
MX346663B (es) | Lineas de celulas de bajo nivel de fucosa y usos de las mismas. | |
NZ586576A (en) | Improved mammalian expression vectors and uses thereof | |
MX357290B (es) | Mejoramiento de la actividad de proteínas que requieren centros fe-s. | |
GEP20217251B (en) | Methods and compositions for rna-directed target dna modification and for rna-directed modulation of transcription | |
EA201291024A1 (ru) | Композиции эндорибонуклеаз и способы их использования | |
PH12016500395A1 (en) | Cell penetrating conjugates and methods of use thereof | |
MY162737A (en) | 4-1bb binding molecules | |
SG10201809566SA (en) | Composition for cleaving a target dna comprising a guide rna specific for the target dna and cas protein-encoding nucleic acid or cas protein, and use thereof | |
MX2015017110A (es) | Integracion dirigida. | |
UA115772C2 (uk) | Композиція програмованого нуклеопротеїнового молекулярного комплексу і спосіб модифікування заданої послідовності нуклеїнової кислоти-мішені | |
MX2020011334A (es) | Cultivo celular metabolicamente optimizado. | |
WO2010128024A3 (en) | Reversibly immortalized cells as well as methods relating hereto | |
EP4282881A3 (en) | Antibodies against csf-1r | |
NZ702084A (en) | System and method for predicting the immunogenicity of a peptide | |
WO2010120518A3 (en) | Sperm cell separation methods and compositions containing sperm cell targeting ligands for use therein | |
MX2015011781A (es) | Medio de cultivo celular y metodos de produccion de anticuerpos. | |
MX357655B (es) | Mediador soluble. | |
WO2009093242A3 (en) | Cell populations for polypeptide analysis and uses of same | |
MX2013014475A (es) | COMPUESTOS DE ENLACE A ß1-ADRENORECEPTOR HUMANO (ß-AR) Y SU USO EN LA MEDICION DE ANTICUERPOS AUTO-ANTI-ß1-AR. | |
UA103624C2 (ru) | Антитело, мишенью которого является полипептид baffr | |
MX353243B (es) | Metodo para incrementar la secrecion de proteinas recombinantes. | |
Bae et al. | Development of causal map for greenhouse gas emission in construction | |
WO2008136253A1 (ja) | ウイルスベクターおよびその利用 | |
NZ719884A (en) | Methods for monitoring responsiveness to anti-smad7 therapy | |
NZ730667A (en) | Methods and compositions for rna-directed target dna modification and for rna-directed modulation of transcription |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG | Grant or registration |