MX344691B - Nuevas fucosiltransferasas y sus aplicaciones. - Google Patents
Nuevas fucosiltransferasas y sus aplicaciones.Info
- Publication number
- MX344691B MX344691B MX2013004055A MX2013004055A MX344691B MX 344691 B MX344691 B MX 344691B MX 2013004055 A MX2013004055 A MX 2013004055A MX 2013004055 A MX2013004055 A MX 2013004055A MX 344691 B MX344691 B MX 344691B
- Authority
- MX
- Mexico
- Prior art keywords
- fucosyltransferases
- applications
- glyco
- alpha
- novel
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/44—Preparation of O-glycosides, e.g. glucosides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/005—Glycopeptides, glycoproteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/01—Hexosyltransferases (2.4.1)
- C12Y204/01214—Glycoprotein 3-alpha-L-fucosyltransferase (2.4.1.214)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
La presente invención se refiere a un método para producir oligosacáridos fucosilados que comprende los siguientes pasos: (a) proveer un polipéptido que presente actividad de alfa-1, 3-fucosisltransferada y con la capacidad de usar lactosa, o un oligosacárido que comprende un residuo de lactosa, como sustrato aceptor; donde el polipéptido consiste en la secuencia de aminoácidos como las que se detallan en SEQ ID NO; 2, SEQ ID NO: 4 ó SEQ ID NO: 6, una secuencia de aminoácidos con al menos una identidad del 95% a la secuencia de aminoácidos como la que se detalla en SEQ ID NO. 2, una secuencia de aminoácidos con al menos una identidad del 95% a la secuencias de aminoácidos como la que se detalla en SEQ ID NO:4, o una secuencia de aminoácidos con al menos una identidad del 95% a la secuencia de aminoácidos como la que se detalla en SEQ ID NO. 6; y poner un contacto el polipéptido que presenta actividad de alfa-1, 3-fucosisltransferasa del paso (a) con una mezcla que comprende un sustrato donante que comprenda un residuo de fucosa y un sustrato aceptor que comprenda lactora o un oligosacárido que comprenda un residuo de lactosa, bajo condiciones apropiadas para que el polipéptido catalice la transferencia de un residuo de fucosa desde el sustrato donante hasta el sustrato aceptor, con lo que podrán producirse oligosacáridos fucosilados.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP10187167.1A EP2439264B1 (en) | 2010-10-11 | 2010-10-11 | Novel fucosyltransferases and their applications |
| PCT/EP2011/067538 WO2012049083A2 (en) | 2010-10-11 | 2011-10-07 | Novel fucosyltransferases and their applications |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MX2013004055A MX2013004055A (es) | 2013-06-05 |
| MX344691B true MX344691B (es) | 2016-12-29 |
Family
ID=43244921
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2013004055A MX344691B (es) | 2010-10-11 | 2011-10-07 | Nuevas fucosiltransferasas y sus aplicaciones. |
Country Status (15)
| Country | Link |
|---|---|
| US (1) | US9441211B2 (es) |
| EP (2) | EP2944690B1 (es) |
| JP (1) | JP6097691B2 (es) |
| CN (1) | CN103201380B (es) |
| AU (1) | AU2011315656B2 (es) |
| BR (1) | BR112013008822A8 (es) |
| DK (2) | DK2944690T3 (es) |
| ES (1) | ES2663627T3 (es) |
| MX (1) | MX344691B (es) |
| MY (1) | MY189569A (es) |
| NZ (1) | NZ609127A (es) |
| PL (1) | PL2944690T3 (es) |
| RU (1) | RU2642307C2 (es) |
| SG (1) | SG189300A1 (es) |
| WO (1) | WO2012049083A2 (es) |
Families Citing this family (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9173910B2 (en) | 2012-02-29 | 2015-11-03 | The General Hospital Corporation | Compositions of microbiota and methods related thereto |
| EP3572521A1 (en) | 2013-09-10 | 2019-11-27 | Jennewein Biotechnologie GmbH | Production of oligosaccharides |
| EP2896628B1 (en) | 2014-01-20 | 2018-09-19 | Jennewein Biotechnologie GmbH | Process for efficient purification of neutral human milk oligosaccharides (HMOs) from microbial fermentation |
| EP2927316B1 (en) | 2014-03-31 | 2018-11-07 | Jennewein Biotechnologie GmbH | Total fermentation of oligosaccharides |
| KR101718681B1 (ko) | 2014-06-23 | 2017-03-22 | 서울대학교산학협력단 | 가용성 단백질 발현량 및 활성이 증대된 헬리코박터 파일로리 유래 α-1,3 푸코실 전달효소의 유전자와 단백질 및 α-1,3 푸코실올리고당 생산에의 응용 |
| AU2015315110B2 (en) * | 2014-09-09 | 2021-04-08 | Glycosyn LLC | Alpha (1,3) fucosyltransferases for use in the production of fucosylated oligosaccharides |
| US9616114B1 (en) | 2014-09-18 | 2017-04-11 | David Gordon Bermudes | Modified bacteria having improved pharmacokinetics and tumor colonization enhancing antitumor activity |
| CA2964480A1 (en) | 2014-10-31 | 2016-05-06 | Whole Biome Inc. | Methods and compositions relating to microbial treatment and diagnosis of disorders |
| EP3050973A1 (en) * | 2015-01-30 | 2016-08-03 | Jennewein Biotechnologie GmbH | Fermentation process for producing monosaccharides in free form from nucleotide-activated sugars |
| KR101794971B1 (ko) * | 2016-02-02 | 2017-11-08 | 서울대학교 산학협력단 | α-1,3-푸코실락토오즈의 대량 생산 방법 |
| CN105695537B (zh) * | 2016-02-29 | 2019-11-19 | 福建农林大学 | 一种降血糖及调节肠道菌群的糖基化浒苔寡糖制备方法 |
| DK3315610T3 (da) | 2016-10-29 | 2021-03-08 | Jennewein Biotechnologie Gmbh | Fremgangsmåde til fremstilling af fucosylerede oligosaccharider |
| US11129906B1 (en) | 2016-12-07 | 2021-09-28 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
| US11180535B1 (en) | 2016-12-07 | 2021-11-23 | David Gordon Bermudes | Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria |
| CN106434591A (zh) * | 2016-12-13 | 2017-02-22 | 福建农林大学 | 一种岩藻糖基转移酶及其应用 |
| CN106434590A (zh) * | 2016-12-13 | 2017-02-22 | 福建农林大学 | 一种岩藻糖基转移酶及其基因工程菌和应用 |
| KR101794972B1 (ko) * | 2017-01-26 | 2017-11-08 | 서울대학교 산학협력단 | α-1,3-푸코실락토오즈의 대량 생산 방법 |
| US11512318B2 (en) * | 2017-04-21 | 2022-11-29 | Seoul National University R&Db Foundation | Method for producing 3-fucosyllactose using Corynebacterium glutamicum |
| EP3425052A1 (en) | 2017-07-07 | 2019-01-09 | Jennewein Biotechnologie GmbH | Fucosyltransferases and their use in producing fucosylated oligosaccharides |
| EP3675882A4 (en) | 2017-08-30 | 2021-07-28 | Pendulum Therapeutics, Inc. | METHODS AND COMPOSITIONS FOR THE TREATMENT OF MICROBIOMA ASSOCIATED DISORDERS |
| WO2019106620A1 (en) * | 2017-11-30 | 2019-06-06 | Glycom A/S | Human milk oligosaccharides and synthetic compositions thereof for microbiota modulation |
| US20220002773A1 (en) * | 2018-12-18 | 2022-01-06 | Inbiose N.V. | Production of 3-fucosyllactose and lactose converting alpha-1,3-fucosyltransferase enzymes |
| EP3751003A1 (en) | 2019-06-12 | 2020-12-16 | Jennewein Biotechnologie GmbH | Production of fucosylated oligosaccharides in bacillus |
| CN116806260A (zh) | 2021-02-08 | 2023-09-26 | 协和发酵生化株式会社 | 具有1,3-岩藻糖基转移酶活性的蛋白和含有岩藻糖的糖质的制造方法 |
| CN115058465A (zh) * | 2022-06-30 | 2022-09-16 | 山东大学 | 一种岩藻糖基化软骨素及其制备方法和应用 |
| CN117187206B (zh) * | 2023-05-19 | 2024-06-18 | 无锡特殊食品与营养健康研究院有限公司 | 肠道微生物来源的岩藻糖基转移酶及其应用 |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2125092C1 (ru) * | 1991-10-15 | 1999-01-20 | Дзе Скриппс Рисерч Инститьют | Способ получения фукозилированного углевода, способ получения фукозилированной сиалилированной углеводной молекулы, реакционная система in vitro |
| EP0621337A1 (en) * | 1993-01-25 | 1994-10-26 | American Cyanamid Company | Codon optimized DNA sequence for insect toxin AaIT |
| US5922577A (en) * | 1995-04-11 | 1999-07-13 | Cytel Corporation | Enzymatic synthesis of glycosidic linkages |
| US5728554A (en) * | 1995-04-11 | 1998-03-17 | Cytel Corporation | Enzymatic synthesis of glycosidic linkages |
| WO1998055630A2 (en) | 1997-06-06 | 1998-12-10 | The Governors Of The University Of Alberta | Alpha-1,3-fucosyltransferase of helicobacter pylori |
| US6500661B1 (en) | 1998-01-15 | 2002-12-31 | Neose Technologies, Inc. | Enzymatic conversion of GDP-mannose to GDP-fucose |
| AT408446B (de) * | 1999-02-18 | 2001-11-26 | Altmann Friedrich Dr | Fucosyltransferase-gen |
| FI19992070A (fi) * | 1999-09-28 | 2001-03-28 | Jari Natunen | Uudet fukosyloidut oligosakkaridit ja menetelmä niiden valmistamiseksi |
| TWI237527B (en) | 2001-11-06 | 2005-08-01 | Delta Optoelectronics Inc | Driver apparatus for a flat-type lamp and driving method for the same |
| US7326770B2 (en) * | 2004-01-22 | 2008-02-05 | Neose Technologies, Inc. | H. pylori fucosyltransferases |
| CA2573113A1 (en) * | 2004-07-26 | 2006-02-09 | Enzon Pharmaceuticals, Inc. | Optimized interferon-beta gene |
| NZ596494A (en) * | 2006-01-13 | 2013-07-26 | Us Gov Nat Inst Health | Codon optimized il-15 and il-15r-alpha genes for expression in mammalian cells |
| EP2099916A2 (en) | 2006-12-22 | 2009-09-16 | Dow AgroSciences LLC | Plant-made west nile virus (wnv) vaccines, vectors and plant codon optimized sequences |
| JP2009095264A (ja) * | 2007-10-15 | 2009-05-07 | Univ Of Ryukyus | コドン最適化ロイヤリシン遺伝子 |
| CN102257128A (zh) * | 2008-12-19 | 2011-11-23 | 詹内怀恩生物技术股份有限公司 | 岩藻糖化化合物的合成 |
-
2010
- 2010-10-11 EP EP15169876.8A patent/EP2944690B1/en active Active
- 2010-10-11 EP EP10187167.1A patent/EP2439264B1/en active Active
- 2010-10-11 DK DK15169876.8T patent/DK2944690T3/en active
- 2010-10-11 DK DK10187167.1T patent/DK2439264T3/en active
- 2010-10-11 PL PL15169876T patent/PL2944690T3/pl unknown
- 2010-10-11 ES ES15169876.8T patent/ES2663627T3/es active Active
-
2011
- 2011-10-07 MY MYPI2013700552A patent/MY189569A/en unknown
- 2011-10-07 BR BR112013008822A patent/BR112013008822A8/pt not_active Application Discontinuation
- 2011-10-07 WO PCT/EP2011/067538 patent/WO2012049083A2/en active Application Filing
- 2011-10-07 SG SG2013025879A patent/SG189300A1/en unknown
- 2011-10-07 NZ NZ609127A patent/NZ609127A/en unknown
- 2011-10-07 CN CN201180049018.5A patent/CN103201380B/zh active Active
- 2011-10-07 RU RU2013120902A patent/RU2642307C2/ru active
- 2011-10-07 JP JP2013532217A patent/JP6097691B2/ja active Active
- 2011-10-07 AU AU2011315656A patent/AU2011315656B2/en active Active
- 2011-10-07 MX MX2013004055A patent/MX344691B/es active IP Right Grant
-
2013
- 2013-04-10 US US13/860,452 patent/US9441211B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| JP2013541952A (ja) | 2013-11-21 |
| MY189569A (en) | 2022-02-17 |
| DK2944690T3 (en) | 2018-04-03 |
| ES2663627T3 (es) | 2018-04-16 |
| AU2011315656A1 (en) | 2013-05-02 |
| SG189300A1 (en) | 2013-05-31 |
| BR112013008822A8 (pt) | 2023-01-31 |
| RU2642307C2 (ru) | 2018-01-24 |
| EP2439264B1 (en) | 2015-06-03 |
| EP2439264A1 (en) | 2012-04-11 |
| EP2944690B1 (en) | 2017-12-20 |
| MX2013004055A (es) | 2013-06-05 |
| EP2944690A2 (en) | 2015-11-18 |
| CN103201380B (zh) | 2018-07-24 |
| PL2944690T3 (pl) | 2018-05-30 |
| NZ609127A (en) | 2014-10-31 |
| AU2011315656B2 (en) | 2016-01-21 |
| EP2944690A3 (en) | 2016-03-09 |
| WO2012049083A3 (en) | 2012-06-14 |
| CN103201380A (zh) | 2013-07-10 |
| WO2012049083A2 (en) | 2012-04-19 |
| RU2013120902A (ru) | 2014-11-20 |
| US20130217068A1 (en) | 2013-08-22 |
| JP6097691B2 (ja) | 2017-03-15 |
| BR112013008822A2 (pt) | 2017-09-19 |
| DK2439264T3 (en) | 2015-08-03 |
| US9441211B2 (en) | 2016-09-13 |
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Legal Events
| Date | Code | Title | Description |
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| FG | Grant or registration |