MX2017005295A - Marcador de seleccion novedoso para transfeccion celular y produccion de proteinas. - Google Patents
Marcador de seleccion novedoso para transfeccion celular y produccion de proteinas.Info
- Publication number
- MX2017005295A MX2017005295A MX2017005295A MX2017005295A MX2017005295A MX 2017005295 A MX2017005295 A MX 2017005295A MX 2017005295 A MX2017005295 A MX 2017005295A MX 2017005295 A MX2017005295 A MX 2017005295A MX 2017005295 A MX2017005295 A MX 2017005295A
- Authority
- MX
- Mexico
- Prior art keywords
- selection marker
- protein production
- cell transfection
- novel selection
- cell lines
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
- C12Y103/05—Oxidoreductases acting on the CH-CH group of donors (1.3) with a quinone or related compound as acceptor (1.3.5)
- C12Y103/05002—Dihydroorotate dehydrogenase (1.3.5.2)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
La presente invención está dentro del campo de la producción industrial de proteínas. La invención proporciona un nuevo sistema de expresión que usa dihidroorotato-deshidrogenasa (DHODH) como un marcador de selección en combinación con leflunomida o uno de sus metabolitos, principalmente para uso en líneas celulares de mamíferos. Asimismo se proporcionan vectores de expresión que codifican la DHODH, líneas celulares que comprenden dichos vectores y métodos para producir proteínas recombinantes.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP14306689 | 2014-10-23 | ||
PCT/EP2015/074550 WO2016062837A1 (en) | 2014-10-23 | 2015-10-22 | Novel selection marker for cell transfection and protein production |
Publications (1)
Publication Number | Publication Date |
---|---|
MX2017005295A true MX2017005295A (es) | 2017-07-28 |
Family
ID=51799064
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2017005295A MX2017005295A (es) | 2014-10-23 | 2015-10-22 | Marcador de seleccion novedoso para transfeccion celular y produccion de proteinas. |
Country Status (11)
Country | Link |
---|---|
US (3) | US10557125B2 (es) |
EP (1) | EP3209770B1 (es) |
CN (1) | CN107109435B (es) |
AU (1) | AU2015334872B2 (es) |
CA (1) | CA2965202C (es) |
DK (1) | DK3209770T3 (es) |
ES (1) | ES2769877T3 (es) |
HU (1) | HUE048525T2 (es) |
MX (1) | MX2017005295A (es) |
SG (1) | SG11201702516UA (es) |
WO (1) | WO2016062837A1 (es) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CL2016001661A1 (es) | 2016-06-29 | 2017-03-03 | Univ Chile | Promotor hibrido de ß-actina (de cricetulus griseus) y citomegalovirus (cmv), con región rica en dinucleótidos, citosina-guanina, vectores, líneas celulares, procedimiento para producir proteínas recombinantes. |
CN111727244B (zh) * | 2018-02-14 | 2023-09-15 | 休斯敦***大学 | 循环肿瘤细胞的通用检测探针 |
WO2020188021A1 (en) | 2019-03-19 | 2020-09-24 | Sanofi | Novel selection marker-comprising cell line and uses thereof for protein production |
TW202227622A (zh) | 2020-09-21 | 2022-07-16 | 法商賽諾菲公司 | 用於生產治療蛋白之高生產重組中國倉鼠卵巢細胞株之世代 |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB8717430D0 (en) | 1987-07-23 | 1987-08-26 | Celltech Ltd | Recombinant dna product |
KR100267720B1 (ko) | 1997-12-29 | 2001-03-02 | 박호군 | 사람 글루타민 합성효소 유전자를 포함하는 발현벡터 및 이를 이용하여 동물세포에서 에리트로포이에틴을 고수율로 생산하는 방법 |
US20030166201A1 (en) * | 2001-04-30 | 2003-09-04 | Jensen Michael C. | Selection systems for genetically modified cells |
GB0808029D0 (en) | 2008-05-01 | 2008-06-11 | F2G Ltd | Antifungal target |
ES2640947T3 (es) | 2009-05-11 | 2017-11-07 | Pfenex, Inc. | Producción de proteínas recombinantes utilizando métodos de selección no antibióticos y la incorporación de aminoácidos no naturales en las mismas |
WO2012109329A2 (en) * | 2011-02-08 | 2012-08-16 | Children's Medical Center Corporation | Methods for treatment of melanoma |
EP2674495A1 (en) * | 2012-06-14 | 2013-12-18 | Sanofi | CHO expression system |
EP2935581A4 (en) | 2012-12-21 | 2016-07-27 | Merck Sharp & Dohme | EXPRESSION VECTORS FOR THE PRODUCTION OF RECOMBINANT PROTEINS IN MAMMALIAN CELLS |
-
2015
- 2015-10-22 AU AU2015334872A patent/AU2015334872B2/en active Active
- 2015-10-22 ES ES15784676T patent/ES2769877T3/es active Active
- 2015-10-22 WO PCT/EP2015/074550 patent/WO2016062837A1/en active Application Filing
- 2015-10-22 US US15/520,840 patent/US10557125B2/en active Active
- 2015-10-22 EP EP15784676.7A patent/EP3209770B1/en active Active
- 2015-10-22 DK DK15784676.7T patent/DK3209770T3/da active
- 2015-10-22 HU HUE15784676A patent/HUE048525T2/hu unknown
- 2015-10-22 CA CA2965202A patent/CA2965202C/en active Active
- 2015-10-22 SG SG11201702516UA patent/SG11201702516UA/en unknown
- 2015-10-22 MX MX2017005295A patent/MX2017005295A/es active IP Right Grant
- 2015-10-22 CN CN201580070024.7A patent/CN107109435B/zh active Active
-
2019
- 2019-12-26 US US16/727,212 patent/US11384342B2/en active Active
-
2022
- 2022-07-08 US US17/811,447 patent/US20230025088A1/en active Pending
Also Published As
Publication number | Publication date |
---|---|
WO2016062837A1 (en) | 2016-04-28 |
US20170335292A1 (en) | 2017-11-23 |
CN107109435B (zh) | 2021-10-22 |
US10557125B2 (en) | 2020-02-11 |
AU2015334872A1 (en) | 2017-06-08 |
CA2965202A1 (en) | 2016-04-28 |
SG11201702516UA (en) | 2017-04-27 |
DK3209770T3 (da) | 2020-02-24 |
US20230025088A1 (en) | 2023-01-26 |
HUE048525T2 (hu) | 2020-07-28 |
AU2015334872B2 (en) | 2021-12-16 |
ES2769877T3 (es) | 2020-06-29 |
US11384342B2 (en) | 2022-07-12 |
US20200199543A1 (en) | 2020-06-25 |
CA2965202C (en) | 2023-02-28 |
EP3209770B1 (en) | 2019-11-27 |
EP3209770A1 (en) | 2017-08-30 |
CN107109435A (zh) | 2017-08-29 |
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