MX2016012492A - Edicion de genoma dirigida en cigotos de animales grandes domesticos. - Google Patents
Edicion de genoma dirigida en cigotos de animales grandes domesticos.Info
- Publication number
- MX2016012492A MX2016012492A MX2016012492A MX2016012492A MX2016012492A MX 2016012492 A MX2016012492 A MX 2016012492A MX 2016012492 A MX2016012492 A MX 2016012492A MX 2016012492 A MX2016012492 A MX 2016012492A MX 2016012492 A MX2016012492 A MX 2016012492A
- Authority
- MX
- Mexico
- Prior art keywords
- nucleic acid
- acid molecule
- genome editing
- zygotes
- genome
- Prior art date
Links
- 241001465754 Metazoa Species 0.000 title abstract 4
- 238000010362 genome editing Methods 0.000 title abstract 2
- 108020004707 nucleic acids Proteins 0.000 abstract 5
- 150000007523 nucleic acids Chemical class 0.000 abstract 5
- 102000039446 nucleic acids Human genes 0.000 abstract 5
- 101710163270 Nuclease Proteins 0.000 abstract 2
- 230000010354 integration Effects 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 239000000203 mixture Substances 0.000 abstract 2
- 108091034117 Oligonucleotide Proteins 0.000 abstract 1
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 238000003776 cleavage reaction Methods 0.000 abstract 1
- 230000004720 fertilization Effects 0.000 abstract 1
- 238000002347 injection Methods 0.000 abstract 1
- 239000007924 injection Substances 0.000 abstract 1
- 230000007017 scission Effects 0.000 abstract 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
- A01K67/0275—Genetically modified vertebrates, e.g. transgenic
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D19/00—Instruments or methods for reproduction or fertilisation
- A61D19/04—Instruments or methods for reproduction or fertilisation for embryo transplantation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4702—Regulators; Modulating activity
- C07K14/4703—Inhibitors; Suppressors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/07—Animals genetically altered by homologous recombination
- A01K2217/072—Animals genetically altered by homologous recombination maintaining or altering function, i.e. knock in
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/10—Mammal
- A01K2227/108—Swine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/124—Animal traits, i.e. production traits, including athletic performance or the like
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Veterinary Medicine (AREA)
- Biomedical Technology (AREA)
- Environmental Sciences (AREA)
- Physics & Mathematics (AREA)
- Animal Behavior & Ethology (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Mycology (AREA)
- Plant Pathology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Toxicology (AREA)
- Immunology (AREA)
- Transplantation (AREA)
- Reproductive Health (AREA)
- Public Health (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
Abstract
Se proporciona un método de edición de genoma dirigida de un animal usando la integración homóloga específica del sitio. Una composición que comprende un oligonucleótido de una sola hebra o molécula de ácido nucleico de doble hebra que comprende una molécula de ácido nucleico de interés y secuencias que flanquean un sitio de segmentación de locus objetivo se inyecta en el cigoto de un animal junto con una nucleasa y una molécula de ácido nucleico de guía que dirige la nucleasa a un locus objetivo. La composición se inyecta en el cigoto después de la fertilización y antes de la formación de un núcleo. La molécula de ácido nucleico de interés se recombina en el genoma con alta eficiencia. El proceso permite la integración de moléculas de ácido nucleico en el genoma de animales en el cual los pronúcleos no pueden ser visualmente observados durante la inyección.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201461970794P | 2014-03-26 | 2014-03-26 | |
| PCT/US2015/022660 WO2015148761A1 (en) | 2014-03-26 | 2015-03-26 | Targeted genome editing in zygotes of domestic large animals |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2016012492A true MX2016012492A (es) | 2017-06-26 |
Family
ID=54196379
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2016012492A MX2016012492A (es) | 2014-03-26 | 2015-03-26 | Edicion de genoma dirigida en cigotos de animales grandes domesticos. |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US10349639B2 (es) |
| EP (1) | EP3122877B1 (es) |
| JP (2) | JP6815986B2 (es) |
| CN (1) | CN106459951A (es) |
| AU (2) | AU2015236033B2 (es) |
| CA (1) | CA2943794C (es) |
| MX (1) | MX2016012492A (es) |
| WO (1) | WO2015148761A1 (es) |
Families Citing this family (37)
| Publication number | Priority date | Publication date | Assignee | Title |
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| EP3613852A3 (en) | 2011-07-22 | 2020-04-22 | President and Fellows of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| CN111500630A (zh) | 2013-04-16 | 2020-08-07 | 瑞泽恩制药公司 | 大鼠基因组的靶向修饰 |
| US20150044192A1 (en) | 2013-08-09 | 2015-02-12 | President And Fellows Of Harvard College | Methods for identifying a target site of a cas9 nuclease |
| US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
| US9322037B2 (en) | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
| US9737604B2 (en) | 2013-09-06 | 2017-08-22 | President And Fellows Of Harvard College | Use of cationic lipids to deliver CAS9 |
| US9228207B2 (en) | 2013-09-06 | 2016-01-05 | President And Fellows Of Harvard College | Switchable gRNAs comprising aptamers |
| US9834791B2 (en) | 2013-11-07 | 2017-12-05 | Editas Medicine, Inc. | CRISPR-related methods and compositions with governing gRNAS |
| US9546384B2 (en) | 2013-12-11 | 2017-01-17 | Regeneron Pharmaceuticals, Inc. | Methods and compositions for the targeted modification of a mouse genome |
| US9068179B1 (en) | 2013-12-12 | 2015-06-30 | President And Fellows Of Harvard College | Methods for correcting presenilin point mutations |
| AU2015298571B2 (en) | 2014-07-30 | 2020-09-03 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
| PT3221457T (pt) | 2014-11-21 | 2019-06-27 | Regeneron Pharma | Métodos e composições para modificação genética visada através da utilização de arn guia emparelhados |
| CN108513575A (zh) | 2015-10-23 | 2018-09-07 | 哈佛大学的校长及成员们 | 核碱基编辑器及其用途 |
| EP3392337B1 (en) * | 2015-12-18 | 2024-03-06 | Japan Science and Technology Agency | Genetic modification non-human organism, egg cells, fertilized eggs, and method for modifying target genes |
| WO2018027078A1 (en) | 2016-08-03 | 2018-02-08 | President And Fellows Of Harard College | Adenosine nucleobase editors and uses thereof |
| CA3033327A1 (en) | 2016-08-09 | 2018-02-15 | President And Fellows Of Harvard College | Programmable cas9-recombinase fusion proteins and uses thereof |
| WO2018039438A1 (en) | 2016-08-24 | 2018-03-01 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| KR20240007715A (ko) | 2016-10-14 | 2024-01-16 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 핵염기 에디터의 aav 전달 |
| US20190330620A1 (en) * | 2016-10-14 | 2019-10-31 | Emendobio Inc. | Rna compositions for genome editing |
| US10745677B2 (en) | 2016-12-23 | 2020-08-18 | President And Fellows Of Harvard College | Editing of CCR5 receptor gene to protect against HIV infection |
| EP3592853A1 (en) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Suppression of pain by gene editing |
| JP2020510439A (ja) | 2017-03-10 | 2020-04-09 | プレジデント アンド フェローズ オブ ハーバード カレッジ | シトシンからグアニンへの塩基編集因子 |
| SG11201908658TA (en) | 2017-03-23 | 2019-10-30 | Harvard College | Nucleobase editors comprising nucleic acid programmable dna binding proteins |
| US11560566B2 (en) | 2017-05-12 | 2023-01-24 | President And Fellows Of Harvard College | Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation |
| US11732274B2 (en) | 2017-07-28 | 2023-08-22 | President And Fellows Of Harvard College | Methods and compositions for evolving base editors using phage-assisted continuous evolution (PACE) |
| US11319532B2 (en) | 2017-08-30 | 2022-05-03 | President And Fellows Of Harvard College | High efficiency base editors comprising Gam |
| CN111757937A (zh) | 2017-10-16 | 2020-10-09 | 布罗德研究所股份有限公司 | 腺苷碱基编辑器的用途 |
| CN110095610A (zh) * | 2018-01-31 | 2019-08-06 | 中国科学院上海生命科学研究院 | 一种高通量蛋白质分析方法及其适用文库 |
| WO2019204530A1 (en) * | 2018-04-17 | 2019-10-24 | University Of Maryland, College Park | Generation of human endodermal organs in pig model using lineage restricted endodermal precursors |
| KR102103104B1 (ko) * | 2018-08-16 | 2020-04-22 | (주)라트바이오 | 유전자 편집된 형질전환 동물 및 형질전환 배아 |
| DE112020001342T5 (de) | 2019-03-19 | 2022-01-13 | President and Fellows of Harvard College | Verfahren und Zusammensetzungen zum Editing von Nukleotidsequenzen |
| US10982187B2 (en) | 2019-03-26 | 2021-04-20 | Genus Plc | Bos taurus variety ‘HO840003150607238’ and methods of use thereof |
| US10975351B2 (en) | 2019-06-17 | 2021-04-13 | Abs Global, Inc. | Bos taurus variety ‘JE840003146074527’ and methods of use therof |
| EP4146804A1 (en) | 2020-05-08 | 2023-03-15 | The Broad Institute Inc. | Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence |
| CN113913468B (zh) * | 2020-07-10 | 2023-09-29 | 北京大学 | 一种蜘蛛的基因编辑方法 |
| CN115044680B (zh) * | 2022-04-22 | 2023-03-14 | 四川省畜牧科学研究院 | 一种与肉兔屠宰性状相关的snp分子标记及其检测引物组、检测试剂盒和应用 |
| JP2023176590A (ja) * | 2022-05-31 | 2023-12-13 | 株式会社ポル・メド・テック | 遺伝子導入ブタの製造方法、体細胞クローニング原料、遺伝子導入ブタ、樹立された体細胞、及び組織ないし臓器 |
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| US5658772A (en) | 1989-12-22 | 1997-08-19 | E. I. Du Pont De Nemours And Company | Site-specific recombination of DNA in plant cells |
| US6720475B1 (en) | 1997-11-18 | 2004-04-13 | Pioneer Hi-Bred International, Inc. | Modified nucleic acid sequence encoding FLP recombinase |
| AUPP249298A0 (en) | 1998-03-20 | 1998-04-23 | Ag-Gene Australia Limited | Synthetic genes and genetic constructs comprising same I |
| US6326193B1 (en) | 1999-11-05 | 2001-12-04 | Cambria Biosciences, Llc | Insect control agent |
| FR2821855B1 (fr) | 2001-03-09 | 2004-04-02 | Cayla | Genes synthetiques et plasmides bacteriens depourvus de cpg |
| US20120276537A1 (en) * | 2009-10-28 | 2012-11-01 | Kuehn Ralf | Homologous recombination in the oocyte |
| CN102770539B (zh) | 2009-12-10 | 2016-08-03 | 明尼苏达大学董事会 | Tal效应子介导的dna修饰 |
| US9528124B2 (en) * | 2013-08-27 | 2016-12-27 | Recombinetics, Inc. | Efficient non-meiotic allele introgression |
| US10058078B2 (en) * | 2012-07-31 | 2018-08-28 | Recombinetics, Inc. | Production of FMDV-resistant livestock by allele substitution |
| PT2896697E (pt) | 2012-12-12 | 2015-12-31 | Massachusetts Inst Technology | Engenharia de sistemas, métodos e composições guia otimizadas para a manipulação de sequências |
| CN105658796B (zh) | 2012-12-12 | 2021-10-26 | 布罗德研究所有限公司 | 用于序列操纵的crispr-cas组分***、方法以及组合物 |
| WO2014093694A1 (en) | 2012-12-12 | 2014-06-19 | The Broad Institute, Inc. | Crispr-cas nickase systems, methods and compositions for sequence manipulation in eukaryotes |
-
2015
- 2015-03-26 CA CA2943794A patent/CA2943794C/en active Active
- 2015-03-26 MX MX2016012492A patent/MX2016012492A/es active IP Right Grant
- 2015-03-26 JP JP2017502925A patent/JP6815986B2/ja active Active
- 2015-03-26 EP EP15769590.9A patent/EP3122877B1/en active Active
- 2015-03-26 US US15/128,595 patent/US10349639B2/en active Active
- 2015-03-26 WO PCT/US2015/022660 patent/WO2015148761A1/en active Application Filing
- 2015-03-26 CN CN201580024317.1A patent/CN106459951A/zh active Pending
- 2015-03-26 AU AU2015236033A patent/AU2015236033B2/en active Active
-
2018
- 2018-05-18 AU AU2018203515A patent/AU2018203515B2/en active Active
-
2019
- 2019-03-06 JP JP2019040959A patent/JP6878482B2/ja active Active
Also Published As
| Publication number | Publication date |
|---|---|
| JP2017510299A (ja) | 2017-04-13 |
| AU2015236033B2 (en) | 2018-06-07 |
| US10349639B2 (en) | 2019-07-16 |
| WO2015148761A1 (en) | 2015-10-01 |
| AU2015236033A1 (en) | 2016-10-13 |
| CA2943794C (en) | 2023-01-24 |
| EP3122877A1 (en) | 2017-02-01 |
| CA2943794A1 (en) | 2015-10-01 |
| JP6878482B2 (ja) | 2021-05-26 |
| CN106459951A (zh) | 2017-02-22 |
| AU2018203515A1 (en) | 2018-06-07 |
| EP3122877B1 (en) | 2023-03-22 |
| AU2018203515B2 (en) | 2020-02-27 |
| US20170172119A1 (en) | 2017-06-22 |
| EP3122877A4 (en) | 2017-08-30 |
| JP2019122390A (ja) | 2019-07-25 |
| JP6815986B2 (ja) | 2021-01-20 |
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| FG | Grant or registration |