MX2016001157A - Circularizacion y amplificacion de acidos nucleicos circulantes asistida de ligasa. - Google Patents

Circularizacion y amplificacion de acidos nucleicos circulantes asistida de ligasa.

Info

Publication number
MX2016001157A
MX2016001157A MX2016001157A MX2016001157A MX2016001157A MX 2016001157 A MX2016001157 A MX 2016001157A MX 2016001157 A MX2016001157 A MX 2016001157A MX 2016001157 A MX2016001157 A MX 2016001157A MX 2016001157 A MX2016001157 A MX 2016001157A
Authority
MX
Mexico
Prior art keywords
amplification
ligase
nucleic acid
stranded dna
assisted nucleic
Prior art date
Application number
MX2016001157A
Other languages
English (en)
Other versions
MX366661B (es
Inventor
Charles Heller Ryan
Richard Nelson John
Leeming Kvam Erik
Original Assignee
Gen Electric
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gen Electric filed Critical Gen Electric
Publication of MX2016001157A publication Critical patent/MX2016001157A/es
Publication of MX366661B publication Critical patent/MX366661B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/501—Ligase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/307—Circular oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/137—Concentration of a component of medium

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

En la presente se proporcionan métodos para la generación y amplificación de un círculo de ADN lineal en un recipiente de reacción único de un ADN lineal sin etapas de purificación intermedias. El círculo de ADN monocatenario es generado a través de una ligación de ADN monocatenario independiente del patrón. Asimismo se proporciona la amplificación del genoma completo de ácidos nucleicos circulantes extraídos de la sangre. También se proporcionan kits para llevar a cabo los métodos.
MX2016001157A 2013-07-26 2014-07-25 Circularización y amplificación de ácidos nucleicos circulantes asistida de ligasa. MX366661B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13/952,040 US9217167B2 (en) 2013-07-26 2013-07-26 Ligase-assisted nucleic acid circularization and amplification
PCT/US2014/048179 WO2015013604A1 (en) 2013-07-26 2014-07-25 Ligase-assisted nucleic acid circularization and amplification

Publications (2)

Publication Number Publication Date
MX2016001157A true MX2016001157A (es) 2016-08-08
MX366661B MX366661B (es) 2019-07-18

Family

ID=51300893

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2016001157A MX366661B (es) 2013-07-26 2014-07-25 Circularización y amplificación de ácidos nucleicos circulantes asistida de ligasa.

Country Status (12)

Country Link
US (1) US9217167B2 (es)
EP (1) EP3024945B1 (es)
JP (2) JP6574178B2 (es)
KR (2) KR20210029845A (es)
CN (1) CN105392901B (es)
AU (1) AU2014292947B2 (es)
CA (1) CA2917452C (es)
ES (1) ES2721204T3 (es)
IL (1) IL243672B (es)
MX (1) MX366661B (es)
SG (1) SG11201600582QA (es)
WO (1) WO2015013604A1 (es)

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US11286519B2 (en) 2013-12-11 2022-03-29 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
WO2017062863A1 (en) 2015-10-09 2017-04-13 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
CA2933343A1 (en) 2013-12-11 2015-06-18 Accuragen, Inc. Compositions and methods for detecting rare sequence variants
US11859246B2 (en) * 2013-12-11 2024-01-02 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
US10335078B2 (en) 2014-08-04 2019-07-02 General Electric Company Device for separation and collection of plasma
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WO2017079114A1 (en) 2015-11-05 2017-05-11 General Electric Company Ligase-assisted nucleic acid circularization and amplification
EP3430154B1 (en) * 2016-03-14 2020-11-11 Rgene, Inc. Hyper-thermostable lysine-mutant ssdna/rna ligases
WO2017201102A1 (en) 2016-05-16 2017-11-23 Accuragen Holdings Limited Method of improved sequencing by strand identification
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JP6966052B2 (ja) 2016-08-15 2021-11-10 アキュラーゲン ホールディングス リミテッド 稀な配列変異体を検出するための組成物および方法
KR102366854B1 (ko) 2016-09-06 2022-02-22 이화여자대학교 산학협력단 회전환 증폭 기반 분자 진단 방법
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WO2019090621A1 (zh) 2017-11-09 2019-05-16 深圳华大智造科技有限公司 钩状探针、核酸连接方法以及测序文库的构建方法
US11203782B2 (en) 2018-03-29 2021-12-21 Accuragen Holdings Limited Compositions and methods comprising asymmetric barcoding
CN109161572B (zh) * 2018-07-05 2021-11-12 中国海洋大学 单链环状核酸及其制备方法和应用
CN112359090A (zh) * 2018-08-07 2021-02-12 深圳市真迈生物科技有限公司 一种核酸片段的连接方法、测序文库的构建方法及其应用
US11195518B2 (en) 2019-03-27 2021-12-07 Sonova Ag Hearing device user communicating with a wireless communication device
CN111321143B (zh) * 2020-02-16 2021-09-17 中国海洋大学 一种制备环状rna的方法
CN111718980A (zh) * 2020-05-14 2020-09-29 中国地质大学（武汉） 一种编辑识别特定核酸链的检测方法
CN115803433A (zh) 2020-07-17 2023-03-14 凯杰贝佛利有限公司 序列偏倚降低的热稳定连接酶
CN114836401A (zh) * 2022-04-24 2022-08-02 中国人民解放军海军军医大学 重组ssDNA核酸环化酶、制备方法及应用

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Also Published As

Publication number Publication date
KR20160034305A (ko) 2016-03-29
US20150031086A1 (en) 2015-01-29
KR102435352B1 (ko) 2022-08-23
AU2014292947A1 (en) 2016-01-28
CA2917452C (en) 2022-12-06
KR20210029845A (ko) 2021-03-16
ES2721204T3 (es) 2019-07-29
EP3024945A1 (en) 2016-06-01
EP3024945B1 (en) 2019-03-27
MX366661B (es) 2019-07-18
WO2015013604A1 (en) 2015-01-29
CA2917452A1 (en) 2015-01-29
SG11201600582QA (en) 2016-02-26
JP2016525360A (ja) 2016-08-25
CN105392901B (zh) 2021-02-02
IL243672A0 (en) 2016-03-31
JP6574178B2 (ja) 2019-09-11
JP2019107015A (ja) 2019-07-04
US9217167B2 (en) 2015-12-22
CN105392901A (zh) 2016-03-09
IL243672B (en) 2019-11-28
AU2014292947B2 (en) 2020-06-18

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