MA40371B1 - Sélectivité efficace des protéines recombinées - Google Patents
Sélectivité efficace des protéines recombinéesInfo
- Publication number
- MA40371B1 MA40371B1 MA40371A MA40371A MA40371B1 MA 40371 B1 MA40371 B1 MA 40371B1 MA 40371 A MA40371 A MA 40371A MA 40371 A MA40371 A MA 40371A MA 40371 B1 MA40371 B1 MA 40371B1
- Authority
- MA
- Morocco
- Prior art keywords
- recombinant proteins
- methods
- expression
- mammalian
- relates
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/005—Glycopeptides, glycoproteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
- C12N2510/02—Cells for production
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
- C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
Cette invention concerne un nouveau système d'expression comprenant un marqueur de mammifère sélectionnable qui favorise les modifications post-traductionnelles souhaitables des glycoprotéines. En particulier, l'invention concerne des procédés et des compositions permettant une expression optimale des protéines recombinées dans des cellules de mammifères par utilisation d'un système de marqueur de sélection basé sur des gènes gpt d'origine mammifère. L'invention concerne également des procédés qui facilitent la sélectivité, des copies d'expression améliorées ainsi qu'un rendement protéique des protéines recombinées amélioré dans des cellules de mammifères, ainsi que des procédés d'utilisation des systèmes d'expression gpt.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201462039416P | 2014-08-19 | 2014-08-19 | |
PCT/US2015/045793 WO2016028838A1 (fr) | 2014-08-19 | 2015-08-19 | Sélectivité efficace des protéines recombinées |
Publications (2)
Publication Number | Publication Date |
---|---|
MA40371A MA40371A (fr) | 2017-06-28 |
MA40371B1 true MA40371B1 (fr) | 2021-03-31 |
Family
ID=55347784
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MA40371A MA40371B1 (fr) | 2014-08-19 | 2015-08-19 | Sélectivité efficace des protéines recombinées |
Country Status (27)
Country | Link |
---|---|
US (4) | US9732357B2 (fr) |
EP (2) | EP3901274A1 (fr) |
JP (3) | JP7115851B2 (fr) |
KR (2) | KR102406976B1 (fr) |
CN (2) | CN107075549B (fr) |
AR (1) | AR102333A1 (fr) |
AU (3) | AU2015305565B9 (fr) |
BR (1) | BR112017003074A2 (fr) |
CA (1) | CA2957036A1 (fr) |
CY (1) | CY1124079T1 (fr) |
DK (1) | DK3183359T3 (fr) |
EA (1) | EA035340B1 (fr) |
ES (1) | ES2849728T3 (fr) |
HR (1) | HRP20210301T1 (fr) |
HU (1) | HUE054069T2 (fr) |
IL (3) | IL287020B (fr) |
LT (1) | LT3183359T (fr) |
MA (1) | MA40371B1 (fr) |
MX (2) | MX2017002203A (fr) |
MY (1) | MY191745A (fr) |
PL (1) | PL3183359T3 (fr) |
PT (1) | PT3183359T (fr) |
RS (1) | RS61371B1 (fr) |
SG (2) | SG10201707859RA (fr) |
SI (1) | SI3183359T1 (fr) |
TW (3) | TWI777374B (fr) |
WO (1) | WO2016028838A1 (fr) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AR102333A1 (es) | 2014-08-19 | 2017-02-22 | Regeneron Pharma | Selectividad eficiente de proteínas recombinadas |
EP3749754A4 (fr) * | 2018-02-08 | 2021-10-20 | Applied Stemcell, Inc. | Procédés de criblage de variant de gène cible |
WO2021079909A1 (fr) | 2019-10-23 | 2021-04-29 | キヤノン株式会社 | Procédé de production d'un article en céramique, et article en céramique |
Family Cites Families (21)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4634665A (en) | 1980-02-25 | 1987-01-06 | The Trustees Of Columbia University In The City Of New York | Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials |
US4656134A (en) | 1982-01-11 | 1987-04-07 | Board Of Trustees Of Leland Stanford Jr. University | Gene amplification in eukaryotic cells |
US5703055A (en) | 1989-03-21 | 1997-12-30 | Wisconsin Alumni Research Foundation | Generation of antibodies through lipid mediated DNA delivery |
US6077835A (en) | 1994-03-23 | 2000-06-20 | Case Western Reserve University | Delivery of compacted nucleic acid to cells |
KR970029803A (ko) | 1995-11-03 | 1997-06-26 | 김광호 | 반도체 메모리장치의 프리차지 회로 |
US5972650A (en) | 1997-06-26 | 1999-10-26 | Brigham And Women's Hospital | Tetracycline repressor regulated mammalian cell transcription and viral replication switch |
US6281005B1 (en) | 1999-05-14 | 2001-08-28 | Copernicus Therapeutics, Inc. | Automated nucleic acid compaction device |
US6919183B2 (en) | 2001-01-16 | 2005-07-19 | Regeneron Pharmaceuticals, Inc. | Isolating cells expressing secreted proteins |
ATE496134T1 (de) | 2002-03-29 | 2011-02-15 | Xoma Technology Ltd | Multigene vectoren und methoden für eine erhöhte expression von rekombinanten polypeptiden |
US8673589B2 (en) | 2002-05-29 | 2014-03-18 | Regeneron Pharmaceuticals, Inc. | Inducible eukaryotic expression system |
US7384744B2 (en) * | 2002-11-29 | 2008-06-10 | Boehringer Ingelheim Pharma Gmbh & Co., Kg | Expression vector, methods for the production of heterologous gene products and for the selection of recombinant cells producing high levels of such products |
JP2005027599A (ja) * | 2003-07-09 | 2005-02-03 | Nara Institute Of Science & Technology | 植物由来遺伝子を用いた遺伝子導入植物の選抜法 |
EP1586585A1 (fr) * | 2004-04-14 | 2005-10-19 | F. Hoffmann-La Roche Ag | Système d'expression pour la production des protéines de fusion IL-15/Fc et leur utilisation |
CA2671365C (fr) | 2006-12-07 | 2020-07-21 | Dow Agrosciences Llc | Utilisation du gene dsm-2 comme agent de selection specifique pour selectionner des plantes resistantes aux herbicides |
PL2150617T3 (pl) | 2007-06-04 | 2015-04-30 | Regeneron Pharma | Wzmocniona ekspresja i regiony stabilności |
KR100927803B1 (ko) * | 2007-11-23 | 2009-11-23 | 한국생명공학연구원 | 식물 형질전환체를 선별하기 위한 마커로서 gpt 유전자 |
ME03057B (fr) * | 2007-12-07 | 2019-01-20 | Zymogenetics Inc | Molécules d'anticorps humanisés spécifiques pour l'IL-31 |
WO2009131681A2 (fr) * | 2008-04-22 | 2009-10-29 | Rutgers, The State University | Compositions de produit de construction e2 de vhc et procédés correspondants |
WO2010077297A1 (fr) * | 2008-12-09 | 2010-07-08 | Halozyme, Inc. | Polypeptides ph20 solubles étendus et leurs applications |
EP2674495A1 (fr) | 2012-06-14 | 2013-12-18 | Sanofi | Système d'expression dans CHO |
AR102333A1 (es) | 2014-08-19 | 2017-02-22 | Regeneron Pharma | Selectividad eficiente de proteínas recombinadas |
-
2015
- 2015-08-14 AR ARP150102631A patent/AR102333A1/es unknown
- 2015-08-17 TW TW110101888A patent/TWI777374B/zh active
- 2015-08-17 TW TW104126657A patent/TWI719945B/zh active
- 2015-08-17 TW TW111129504A patent/TWI832364B/zh active
- 2015-08-19 SI SI201531487T patent/SI3183359T1/sl unknown
- 2015-08-19 BR BR112017003074-8A patent/BR112017003074A2/pt active Search and Examination
- 2015-08-19 EA EA201790241A patent/EA035340B1/ru unknown
- 2015-08-19 MY MYPI2017700248A patent/MY191745A/en unknown
- 2015-08-19 WO PCT/US2015/045793 patent/WO2016028838A1/fr active Application Filing
- 2015-08-19 ES ES15833421T patent/ES2849728T3/es active Active
- 2015-08-19 HU HUE15833421A patent/HUE054069T2/hu unknown
- 2015-08-19 EP EP20211902.0A patent/EP3901274A1/fr active Pending
- 2015-08-19 IL IL287020A patent/IL287020B/en unknown
- 2015-08-19 KR KR1020177006620A patent/KR102406976B1/ko active IP Right Grant
- 2015-08-19 CA CA2957036A patent/CA2957036A1/fr active Pending
- 2015-08-19 AU AU2015305565A patent/AU2015305565B9/en active Active
- 2015-08-19 JP JP2017509733A patent/JP7115851B2/ja active Active
- 2015-08-19 PT PT158334219T patent/PT3183359T/pt unknown
- 2015-08-19 KR KR1020227018877A patent/KR102556383B1/ko active IP Right Grant
- 2015-08-19 MA MA40371A patent/MA40371B1/fr unknown
- 2015-08-19 CN CN201580044100.7A patent/CN107075549B/zh active Active
- 2015-08-19 US US14/829,834 patent/US9732357B2/en active Active
- 2015-08-19 PL PL15833421T patent/PL3183359T3/pl unknown
- 2015-08-19 CN CN202110402963.4A patent/CN113293139A/zh active Pending
- 2015-08-19 EP EP15833421.9A patent/EP3183359B1/fr active Active
- 2015-08-19 SG SG10201707859RA patent/SG10201707859RA/en unknown
- 2015-08-19 LT LTEP15833421.9T patent/LT3183359T/lt unknown
- 2015-08-19 DK DK15833421.9T patent/DK3183359T3/da active
- 2015-08-19 SG SG11201701121UA patent/SG11201701121UA/en unknown
- 2015-08-19 RS RS20210103A patent/RS61371B1/sr unknown
- 2015-08-19 MX MX2017002203A patent/MX2017002203A/es unknown
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2017
- 2017-02-09 IL IL250531A patent/IL250531B/en unknown
- 2017-02-17 MX MX2021009154A patent/MX2021009154A/es unknown
- 2017-07-31 US US15/664,444 patent/US10457959B2/en active Active
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2019
- 2019-09-10 US US16/566,253 patent/US11085053B2/en active Active
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2020
- 2020-07-17 AU AU2020205336A patent/AU2020205336B2/en active Active
- 2020-08-05 JP JP2020132874A patent/JP7260510B2/ja active Active
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2021
- 2021-02-08 CY CY20211100104T patent/CY1124079T1/el unknown
- 2021-02-23 HR HRP20210301TT patent/HRP20210301T1/hr unknown
- 2021-07-02 US US17/366,239 patent/US20210340564A1/en active Pending
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2022
- 2022-08-04 IL IL295361A patent/IL295361B2/en unknown
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2023
- 2023-02-10 AU AU2023200752A patent/AU2023200752B2/en active Active
- 2023-04-06 JP JP2023061971A patent/JP2023076667A/ja active Pending
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