KR940014767A - How to separate cells from amino acid and nucleic acid fermentation broth - Google Patents
How to separate cells from amino acid and nucleic acid fermentation broth Download PDFInfo
- Publication number
- KR940014767A KR940014767A KR1019920023938A KR920023938A KR940014767A KR 940014767 A KR940014767 A KR 940014767A KR 1019920023938 A KR1019920023938 A KR 1019920023938A KR 920023938 A KR920023938 A KR 920023938A KR 940014767 A KR940014767 A KR 940014767A
- Authority
- KR
- South Korea
- Prior art keywords
- fermentation broth
- nucleic acid
- amino acid
- separate cells
- flocculant
- Prior art date
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/02—Separating microorganisms from their culture media
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/06—Alanine; Leucine; Isoleucine; Serine; Homoserine
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/14—Glutamic acid; Glutamine
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/44—Preparation of O-glycosides, e.g. glucosides
- C12P19/445—The saccharide radical is condensed with a heterocyclic radical, e.g. everninomycin, papulacandin
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Abstract
본 발명에 따르면 통상의 방법으로 얻은 아미노산 또는 핵산 발효액으로부터 균체를 효율적으로 제거하는 새로운 방법에 관한 것이다. 본 발명의 방법은 유기계 응집제를 50~300ppm 넣고 50~80℃로 30분~2시간 가열 후 분리시 씨크너를 사용하여 연속적으로 균체 분리 상징액과 균체 포함액을 분리함을 특징으로 하는데 본 발명의 방법에 의해 균체 제거를 위한 기계적 처리량을 18~31%로 줄일 수 있고, 최종적으로 회수되는 아미노산 또는 핵산의 수율을 증가시킬 수 있다.The present invention relates to a new method for efficiently removing cells from amino acids or nucleic acid fermentation broth obtained by conventional methods. The method of the present invention is characterized in that after separating 50 ~ 300ppm of organic flocculant and heating for 30 minutes to 2 hours at 50 ~ 80 ℃ using a thinner to separate the cell separation supernatant and the cell containing liquid continuously. By this, the mechanical throughput for cell removal can be reduced to 18-31%, and the yield of amino acid or nucleic acid finally recovered can be increased.
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.
제1도는 본원 발명의 공정도이다.1 is a process diagram of the present invention.
Claims (8)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1019920023938A KR960006578B1 (en) | 1992-12-11 | 1992-12-11 | Separating method microorganisms from amino acid and nucleotide fermenting liquid |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1019920023938A KR960006578B1 (en) | 1992-12-11 | 1992-12-11 | Separating method microorganisms from amino acid and nucleotide fermenting liquid |
Publications (2)
Publication Number | Publication Date |
---|---|
KR940014767A true KR940014767A (en) | 1994-07-19 |
KR960006578B1 KR960006578B1 (en) | 1996-05-20 |
Family
ID=19345250
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1019920023938A KR960006578B1 (en) | 1992-12-11 | 1992-12-11 | Separating method microorganisms from amino acid and nucleotide fermenting liquid |
Country Status (1)
Country | Link |
---|---|
KR (1) | KR960006578B1 (en) |
-
1992
- 1992-12-11 KR KR1019920023938A patent/KR960006578B1/en not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
KR960006578B1 (en) | 1996-05-20 |
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