KR940014767A - How to separate cells from amino acid and nucleic acid fermentation broth - Google Patents

How to separate cells from amino acid and nucleic acid fermentation broth Download PDF

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Publication number
KR940014767A
KR940014767A KR1019920023938A KR920023938A KR940014767A KR 940014767 A KR940014767 A KR 940014767A KR 1019920023938 A KR1019920023938 A KR 1019920023938A KR 920023938 A KR920023938 A KR 920023938A KR 940014767 A KR940014767 A KR 940014767A
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South Korea
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fermentation broth
nucleic acid
amino acid
separate cells
flocculant
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KR1019920023938A
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Korean (ko)
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KR960006578B1 (en
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박한선
강기권
이계철
황이남
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유영학
주식회사 미 원
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02—Separating microorganisms from their culture media
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/06—Alanine; Leucine; Isoleucine; Serine; Homoserine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/14—Glutamic acid; Glutamine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/445—The saccharide radical is condensed with a heterocyclic radical, e.g. everninomycin, papulacandin

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

본 발명에 따르면 통상의 방법으로 얻은 아미노산 또는 핵산 발효액으로부터 균체를 효율적으로 제거하는 새로운 방법에 관한 것이다. 본 발명의 방법은 유기계 응집제를 50~300ppm 넣고 50~80℃로 30분~2시간 가열 후 분리시 씨크너를 사용하여 연속적으로 균체 분리 상징액과 균체 포함액을 분리함을 특징으로 하는데 본 발명의 방법에 의해 균체 제거를 위한 기계적 처리량을 18~31%로 줄일 수 있고, 최종적으로 회수되는 아미노산 또는 핵산의 수율을 증가시킬 수 있다.The present invention relates to a new method for efficiently removing cells from amino acids or nucleic acid fermentation broth obtained by conventional methods. The method of the present invention is characterized in that after separating 50 ~ 300ppm of organic flocculant and heating for 30 minutes to 2 hours at 50 ~ 80 ℃ using a thinner to separate the cell separation supernatant and the cell containing liquid continuously. By this, the mechanical throughput for cell removal can be reduced to 18-31%, and the yield of amino acid or nucleic acid finally recovered can be increased.

Description

아미노산 및 핵산 발효액으로부터 균체를 분리하는 방법How to separate cells from amino acid and nucleic acid fermentation broth

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1도는 본원 발명의 공정도이다.1 is a process diagram of the present invention.

Claims (8)

통상의 발효법에 의해 얻은 아미노산 또는 핵산 발효액을 약산성으로 조절한 후 유기계 응집제를 첨가하고 가온하여 반응시킨 후 균체를 응집시켜 원추형 씨크너(thickener)에 의해 연속적으로 균체와 상징액을 분리하는 방법.A method of separating the cells and the supernatant continuously by conical thickener after controlling the amino acid or nucleic acid fermentation broth obtained by the conventional fermentation method to weak acidity, and then adding the organic flocculant and heating to react. 제1항에 있어서, 유기계 응집제로 폴리아크릴레이트 나트륨 (Sodium polyacrylate)계 또는 폴리아크릴아미드나트륨 (Sodium polyacryamide)계 응집제를 사용함을 특징으로 하는 방법.The method of claim 1, wherein a sodium polyacrylate-based or sodium polyacryamide-based flocculant is used as the organic flocculant. 제1항에 있어서, 발효액을 pH3.0~5.0으로 조절하는 방법.The method of claim 1, wherein the fermentation broth is adjusted to pH 3.0-5.0. 제1항에 있어서, 발효액을 유기응집체를 50~300ppm 첨가하는 방법.The method according to claim 1, wherein the fermentation broth is added with 50 to 300 ppm of an organic aggregate. 제1항에 있어서, 발효액을 50~80℃로 가열하는 방법.The method of claim 1, wherein the fermentation broth is heated to 50 to 80 ° C. 제1항에 있어서, 발효액을 0.5~2시간 가열하는 방법.The method according to claim 1, wherein the fermentation broth is heated for 0.5 to 2 hours. 제1항에 있어서, 아미노산은 글루타민산, 페닐알라닌, 라이신 임을 특징으로 하는 방법.The method of claim 1 wherein the amino acid is glutamic acid, phenylalanine, lysine. 제1항에 있어서, 핵산은 5′-이노신산나트륨 및 5′-구아닐산나트륨임을 특징으로 하는 방법.The method of claim 1, wherein the nucleic acids are 5′-soinosinate and 5′-sodium guanylate. ※참고사항:최초출원 내용에 의하여 공개하는 것임.※ Note: This is to be disclosed based on the first application.
KR1019920023938A 1992-12-11 1992-12-11 Separating method microorganisms from amino acid and nucleotide fermenting liquid KR960006578B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019920023938A KR960006578B1 (en) 1992-12-11 1992-12-11 Separating method microorganisms from amino acid and nucleotide fermenting liquid

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019920023938A KR960006578B1 (en) 1992-12-11 1992-12-11 Separating method microorganisms from amino acid and nucleotide fermenting liquid

Publications (2)

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KR940014767A true KR940014767A (en) 1994-07-19
KR960006578B1 KR960006578B1 (en) 1996-05-20

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