JPS5913276B2 - Method for purifying factory waste liquid using yeast - Google Patents
Method for purifying factory waste liquid using yeastInfo
- Publication number
- JPS5913276B2 JPS5913276B2 JP51077344A JP7734476A JPS5913276B2 JP S5913276 B2 JPS5913276 B2 JP S5913276B2 JP 51077344 A JP51077344 A JP 51077344A JP 7734476 A JP7734476 A JP 7734476A JP S5913276 B2 JPS5913276 B2 JP S5913276B2
- Authority
- JP
- Japan
- Prior art keywords
- waste liquid
- amino acid
- cod
- bod
- yeast
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 239000007788 liquid Substances 0.000 title claims description 43
- 239000002699 waste material Substances 0.000 title claims description 17
- 238000000034 method Methods 0.000 title claims description 7
- 240000004808 Saccharomyces cerevisiae Species 0.000 title claims description 5
- 150000001413 amino acids Chemical class 0.000 claims description 20
- 239000002921 fermentation waste Substances 0.000 claims description 11
- 241000223230 Trichosporon Species 0.000 claims description 9
- 238000012258 culturing Methods 0.000 claims 1
- 235000001014 amino acid Nutrition 0.000 description 19
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 18
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 12
- 235000011194 food seasoning agent Nutrition 0.000 description 9
- 238000004519 manufacturing process Methods 0.000 description 8
- 229910052757 nitrogen Inorganic materials 0.000 description 6
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 5
- 230000001580 bacterial effect Effects 0.000 description 5
- 238000000855 fermentation Methods 0.000 description 4
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 238000005273 aeration Methods 0.000 description 3
- 230000004151 fermentation Effects 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 241000223233 Cutaneotrichosporon cutaneum Species 0.000 description 2
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 235000013922 glutamic acid Nutrition 0.000 description 2
- 239000004220 glutamic acid Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 150000007524 organic acids Chemical class 0.000 description 2
- 235000005985 organic acids Nutrition 0.000 description 2
- 235000018102 proteins Nutrition 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 239000010802 sludge Substances 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 241000222120 Candida <Saccharomycetales> Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 241000178290 Geotrichum fermentans Species 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 1
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 1
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- 241000927735 Penaeus Species 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 1
- 239000004473 Threonine Substances 0.000 description 1
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- -1 inleucine Chemical compound 0.000 description 1
- 239000010812 mixed waste Substances 0.000 description 1
- 235000013379 molasses Nutrition 0.000 description 1
- 230000003472 neutralizing effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000004474 valine Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Seasonings (AREA)
Description
【発明の詳細な説明】
アミノ酸発酵廃液及びアミノ酸調味液製造廃液はいずれ
も活性汚泥法、メタン発酵法、濃縮焼却法等により処理
することができる。DETAILED DESCRIPTION OF THE INVENTION Both the amino acid fermentation waste liquid and the amino acid seasoning liquid production waste liquid can be treated by an activated sludge method, a methane fermentation method, a concentration incineration method, or the like.
しかしながら、これらの廃液は数千ｐｌ）ｍ ｆｉいし
数万ｐｐｍのＢＯＤを含む高濃度の廃液であるため活性
汚泥で処理する場合には予め大量の水で希釈しなければ
ならず、かつ大きな設備が必要である。However, these waste liquids are highly concentrated waste liquids containing several thousand pl) mfi to tens of thousands of ppm of BOD, so if they are to be treated with activated sludge, they must be diluted with a large amount of water in advance, and large equipment is required. is necessary.
また、メタン発酵のような嫌気発酵で処理する場合には
、一般に長時間を要するため設備面で問題がある。Furthermore, when processing by anaerobic fermentation such as methane fermentation, it generally takes a long time, which poses problems in terms of equipment.
また、濃縮して焼却その他の処理を行う場合には、濃縮
のために多量のエネルギーを必要とするので適当でない
。Further, in the case of concentrating and incinerating or other processing, it is not suitable because a large amount of energy is required for concentration.
アミノ酸発酵廃液、アミノ酸調味液製造廃液に含まれる
ＢＯＤはい（れも多くの微生物によっである程度資化さ
れることが考えられる。BOD contained in amino acid fermentation waste liquid and amino acid seasoning liquid manufacturing waste liquid (both are thought to be assimilated to some extent by many microorganisms).
しかしながら、たとえばアミノ酸発酵廃液のＢＯＤは発
酵に用いられる微生物によって培地中の栄養分のうち資
化されやすいものが資化された残りを中心とするもので
あり、このようなりＯＤを微生物によって効率よく浄化
するのは容易なことではない。However, for example, the BOD of amino acid fermentation waste liquid mainly consists of the remainder of the easily assimilated nutrients in the culture medium by the microorganisms used in fermentation. It's not an easy thing to do.
本発明者らは、高濃度のＢＯＤを含有するアミノ酸発酵
廃液及びアミノ酸調味液製造廃液の浄化方法について鋭
意研究した結果、トリコスポロン属の酵母が極めて高い
効率でしかも極めて高い速度で上記廃液中の有機物質を
資化することを見いだした。As a result of intensive research into methods for purifying amino acid fermentation waste liquids and amino acid seasoning liquid manufacturing waste liquids containing high concentrations of BOD, the present inventors found that yeast of the genus Trichosporon was able to efficiently and rapidly remove organic matter in the waste liquids. It was discovered that substances can be assimilated.
本発明はこれらの知見に基いて完成されるに到ったもの
である。The present invention has been completed based on these findings.
本発明のトリコスポロン属の酵母は例えば以下のものが
ある。Examples of the yeast of the genus Trichosporon of the present invention include the following.
トリコスポロン・インフェスタンスＡＪ ５１５３ＣＢ
Ｓ ２５３０ （Ｔｒｉｃｈｏｓｐｏｒｏｎ １ｎｆｅ
ｓｔａｎｓ ）同 ブラシカニ ＡＪ４８３３
ＣＢＳ ６３８２ （Ｔｒｉｃｈｏｓｐｏｒｏｎ ｂｒ
ａｓｓｉｃａｅ）同 フェルメンタンス ＡＪ
５１５２ＣＢＳ ２５２９ （Ｔｒ−ｆｅｒｍｅｎｔ
ａｎｓ）同 クタネウム ＡＪ１４０７６ＣＢ
Ｓ２４６６ （Ｔｒ−ｃｕｔａｎｅｕｍ）同
クタネウム・パル・ペネアウスＡＪ５１２１ ＣＢ５
２４９７（Ｔｒ”ｃｕｔａｎｅｕｍＶａ ｒ ＋ｐｅｎ
ｅａｕｓ ）
同 ベニシラタム ＡＪ５１０６ＣＢＳ５５８
６ （Ｔｒ−ｐｅｎｉｃｉｌｌａｔｕｍ）トリコスポロ
ン・フエニカム ＡＪ１４２００ＣＢＳ５９２８（Ｔｒ
−Ｆｅｎｎｉｃｕｍ）本発明のアミノ酸発酵廃液はグル
タミン酸、リシン、バリン、インロイシン、スレオニン
、フロリン等蛋白構成４分であるアミノ酸の、炭素源と
してデンプン加水分解物、廃糖蜜などの糖類を使用して
得られた発酵液から目的物を分離した廃液であって、ア
ミノ酸、有機酸、糖類、無機塩、菌体、等を含み、ＢＯ
Ｄｌｏ、０００〜３０，０００ｐｐｍ。Trichosporon infestans AJ 5153CB
S 2530 (Trichosporon 1nfe
stans ) same brush crab AJ4833 CBS 6382 (Trichosporon br
assicae) Fermentans AJ
5152CBS 2529 (Tr-ferment
ans) Kutaneum AJ14076CB
S2466 (Tr-cutaneum) same
Cutaneum Pal Penaeus AJ5121 CB5
2497(Tr”cutaneumVar + pen
eas) Venicillatum AJ5106CBS558
6 (Tr-penicillatum) Trichosporon fenicum AJ14200CBS5928 (Tr-penicillatum)
-Fenicum) The amino acid fermentation waste liquid of the present invention is obtained by using saccharides such as starch hydrolyzate and blackstrap molasses as a carbon source of amino acids that make up the protein composition such as glutamic acid, lysine, valine, inleucine, threonine, and florin. This waste liquid is obtained by separating the target product from the fermented liquid, and contains amino acids, organic acids, sugars, inorganic salts, bacterial cells, etc., and contains BO.
Dlo, 000-30,000 ppm.
ＣＯＤ−Ｍｎ ７，０００〜２０，０００ｐｐｍ、全窒
素３．０００〜７，０００ｐｐ［［１１アンモニア態窒
素２．５００〜５，０００ｐＩ）［ｎ程度のものである
。COD-Mn 7,000 to 20,000 ppm, total nitrogen 3.000 to 7,000 ppm [[11 ammonia nitrogen 2.500 to 5,000 pI] [n].
又、アミノ酸調味液製造廃液とは、脱脂大豆を塩酸分解
したのち不溶残渣を濾過して除去した液を中和、脱色し
てアミノ酸調味液を製造する際に発生する廃液である。Furthermore, the amino acid seasoning liquid production waste liquid is a waste liquid generated when an amino acid seasoning liquid is produced by neutralizing and decolorizing the liquid obtained by decomposing defatted soybeans with hydrochloric acid and then filtering and removing insoluble residues.
そしてこの廃液は、アミノ酸、有機酸、色素等を含み、
ＢＯＤ５，０００〜２８，０００ｐｐｍ、 ＣＯＤ−Ｍ
ｎ ２，０００〜１７．００ Ｑｐｐｍ全窒素１，００
０〜３．０００ｐｐｍ程度である。This waste liquid contains amino acids, organic acids, pigments, etc.
BOD5,000-28,000ppm, COD-M
n 2,000-17.00 Qppm total nitrogen 1,00
It is about 0 to 3.000 ppm.
このような廃液に、上記トリコスポロン属の微生物を接
種し、望ましくはｐＨ３ないし６、温度２５ないし４０
℃に制御しつつ、好気的条件で培養する。The above-mentioned Trichosporon microorganisms are inoculated into such waste liquid, and the pH is preferably 3 to 6 and the temperature is 25 to 40.
Culture in aerobic conditions with controlled temperature.
連続培養方式で処理する場合には、ＢＯＤ容積負荷で２
０ないし６０ｋｇ／ｉＤ程度が適当である。When processing in a continuous culture method, the BOD volume loading is 2
Approximately 0 to 60 kg/iD is appropriate.
この場合、微生物の濃度は５．ＱＱＱｐｐｍ以上に保つ
ことが望ましい。In this case, the concentration of microorganisms is 5. It is desirable to keep it above QQQppm.
所望により培養液から、微生物菌体を常法により分離す
る。If desired, microbial cells are separated from the culture solution by a conventional method.
分離した微生物菌体は、飼料原料、肥料原料、その他蛋
白源等として使用できる。The isolated microbial cells can be used as feed materials, fertilizer materials, other protein sources, etc.
かくしてＢＯＤの９０％以上、ＣＯＤ−Ｍｎの７５％以
上が本発明の方法で除去できる。Thus, more than 90% of BOD and more than 75% of COD-Mn can be removed by the method of the present invention.
実施例 １
アミノ酸発酵廃液、核酸発酵廃液、及びアミノ酸調味液
製造廃液を混合したＢＯＤ２８，０００ｐｐｍ。Example 1 A mixture of amino acid fermentation waste liquid, nucleic acid fermentation waste liquid, and amino acid seasoning liquid manufacturing waste liquid had a BOD of 28,000 ppm.
ＣＯＤ −Ｍ ｎ １５．４０ Ｑｐｐｍ 、 ｐｉ−
１４，９の液を５倍希釈したのちに５０ｍ１宛５００ｍ
１容振盪フラスコに分注し、これに表１記載の菌株を接
種して３１，５℃で６８時間振盪培養した。COD -Mn 15.40 Qppm, pi-
After diluting the solution 14.9 5 times, 50ml to 500ml
The mixture was dispensed into 1-volume shaking flasks, inoculated with the bacterial strains listed in Table 1, and cultured with shaking at 31.5°C for 68 hours.
４５時間および６８時間後のＣＯＤ−Ｍｎを測定した結
果を表１に示す。Table 1 shows the results of measuring COD-Mn after 45 and 68 hours.
実施例 ２
アミノ酸発酵廃液、核酸発酵廃液、及びアミノ酸調味液
製造廃液を混合したＢＯＤ １９１０ Ｑｐｐｍ。Example 2 BOD 1910 Qppm is a mixture of amino acid fermentation waste liquid, nucleic acid fermentation waste liquid, and amino acid seasoning liquid manufacturing waste liquid.
ＣＯＤ−Ｍｎ １２，００ Ｑｐｐｍ、全窒素５，１１
００ｐ１）、アンモニア態窒素２，６００ｐｐｍ１ｐＨ
３の液を用いて連続培養を行なった。COD-Mn 12,00 Qppm, total nitrogen 5,11
00p1), ammonia nitrogen 2,600ppm1pH
Continuous culture was performed using the solution No. 3.
上記混合廃液５５０１を培養槽に張込み、ＮａＯＨを用
いてｐＨを４．５に調整後トリコスポロン・クタネウム
ＣＢ５２４６６を接種し、ＢＯＤ容積負荷５５ｋｇ／ｍ
’Ｄで運転を行なった。The above mixed waste liquid 5501 was poured into a culture tank, the pH was adjusted to 4.5 using NaOH, and Trichosporon cutaneum CB52466 was inoculated, and the BOD volume load was 55 kg/m.
I drove in 'D.
培養中、ｐＨはＮａＯＨを用いて４．５にそして温度は
３４℃に保ち、かつ通気撹拌（１／２ＶＶＭ、３２０Ｒ
ＰＭ）を行なった。During the cultivation, the pH was maintained at 4.5 using NaOH and the temperature at 34°C, and with aeration and stirring (1/2 VVM, 320R).
PM) was carried out.
定常状態に達したときの処理液の菌体濃度は乾燥重量で
８，５ｇ／ｌであり、ＢＯＤおよびＣＯＤ−Ｍｎはそれ
ぞれｃ＋９ｏｐｐｍ（除去率９５％）および２，４００
ｐ四（除去率８０％）であった。When the steady state was reached, the bacterial cell concentration of the treated liquid was 8.5 g/l on a dry weight basis, and BOD and COD-Mn were c + 9 oppm (removal rate 95%) and 2,400 ppm, respectively.
p4 (removal rate 80%).
実施例 ３
ＢＯＤ ２０，７０ Ｑｐｐｍ、 ＣＯＤ−Ｍｎ １３
，７００四、全窒素７．４０ Ｑｐｐｍ、アンモニア態
窒素５，２００ｐｐＩＩ１１ｐＨ２，３のグルタミン酸
発酵廃液４００ｍ１を培養槽に張込み、ＮａＯＨでｐＨ
を５．０に調整後トリコスポロン・フェルメンタンスＣ
Ｂ５２５２９を接種し、ＢＯＤ容積負荷５５ｋｇ／ｍ″
Ｄで連続培養を行なった。Example 3 BOD 20,70 Qppm, COD-Mn 13
, 7004, total nitrogen 7.40 Qppm, ammonia nitrogen 5,200 ppm II 11 pH 2,3 400 ml of glutamic acid fermentation waste liquid was charged into a culture tank, and the pH was adjusted with NaOH.
Trichosporon fermentans C after adjusting to 5.0
Inoculated with B52529, BOD volume load 55 kg/m''
Continuous culture was performed in D.
培養中、ｐＨはＮａＯＨでｐＨを５．０にそして温度を
３５°Ｃに調整し、かつ通気撹拌（ｌ／２ ＶＶＭ 、
１．２０ ＯＲＰＭ ）を行なった。During the cultivation, the pH was adjusted to 5.0 with NaOH and the temperature was adjusted to 35 °C, and with aeration and stirring (l/2 VVM,
1.20 ORPM) was performed.
定常状態に達したときの処理液の菌体濃度は乾燥重量で
１０９／ｌであり、ＢＯＤおよびＣＯＤ−Ｍｎはそれぞ
れ１，０００四および２，６００１１ｐ［ｌＩであった
。When a steady state was reached, the bacterial cell concentration of the treated liquid was 109/l on a dry weight basis, and the BOD and COD-Mn were 1,000 and 2,600 p[lI, respectively.
除去率はＢＯＤ９５％、ＣＯＤ−Ｍｎ８１％になる。The removal rate is 95% for BOD and 81% for COD-Mn.
実施例 ４
ｐＨ２，８のアミノ酸調味液製造廃液をＮａＯＨを用い
てｐＨ５，６に調整したＢＯＤ２７７０ Ｑｐｐｍ。Example 4 BOD2770 Qppm obtained by adjusting the pH of amino acid seasoning liquid manufacturing waste liquid of pH 2.8 to pH 5.6 using NaOH.
ＣＯＤ−Ｍｎ １７２００ｐｐｍ、全窒素２８７０ｐＩ
ＩＩＩｌおよびアンモニア態窒素１５０ｐｐｍの液なら
びにこれを約１／３および１／１０に希釈した液ＣＯＤ
−Ｍ ｎ５５９０ｐ１）［１１および１７ｓｏｐｐｍ
）を１２０℃２０分加熱殺菌した。COD-Mn 17200ppm, total nitrogen 2870pI
IIIl and ammonia nitrogen 150 ppm solution and a solution diluted to about 1/3 and 1/10 COD
-M n5590p1) [11 and 17 soppm
) was heat sterilized at 120°C for 20 minutes.
これらの液を５ｍｌ宛試験管に分注し、表２に記載した
菌株を接種して３１℃で５１時間振盪培養した。These solutions were dispensed into 5 ml test tubes, inoculated with the strains listed in Table 2, and cultured with shaking at 31° C. for 51 hours.
培養液のＣＯＤ −Ｍ ｎを測定した結果を表２に示す
。Table 2 shows the results of measuring COD-Mn of the culture solution.
ＢＯＤ２６６０ ｏｐｐｍ、 ＣＯＤ−Ｍｎ １４６
００ｐｐＨ１１全窒素３１００ｐ戸、アンモニア態窒素
２３０ｐｐｍ、ｐＨ３，９のアミノ酸調味液製造廃液１
０１を培養槽に張込み、ＮａＯＨでｐＨ４，０に調整後
トリコスポロン・クタネウムＣＢ５２４６６、同インフ
ェスタンスＣＢ５２５３０およびキャンデイダ・リボリ
テイカＡＪ１４１０１ ＩＦＯ１５４８を接種し、ＢＯ
Ｄ容積負荷４６ ｋｇ／ ｍ” Ｄで連続培養を行なっ
た。BOD2660 oppm, COD-Mn 146
00ppph11 total nitrogen 3100p, ammonia nitrogen 230ppm, pH 3.9 amino acid seasoning liquid manufacturing waste liquid 1
01 was placed in a culture tank, adjusted to pH 4.0 with NaOH, and inoculated with Trichosporon cutaneum CB52466, Trichosporon infestans CB52530, and Candida ribolitica AJ14101 IFO1548.
Continuous culture was carried out at a D volume load of 46 kg/m''D.
培養条件はｐＨ４，０、温度３５℃、通気ＩＡＶＶＭお
よび撹拌７５０ ＲＰＭとした。The culture conditions were pH 4.0, temperature 35°C, aeration IAVVM and agitation 750 RPM.
定常状態に達したときの処理液の菌体濃度は乾燥重量で
１２．１９／ｌであり、ＢＯＤおよびＣＯＤ−Ｍｎはそ
れぞれ１１０７０ｐｐおよび３５５０ｐｐｍであった。When a steady state was reached, the bacterial cell concentration of the treated liquid was 12.19/l on a dry weight basis, and BOD and COD-Mn were 11,070 ppm and 3,550 ppm, respectively.
除去率はＢＯＤ９６％、ＣＯＤ−Ｍｎ７６％になる。The removal rate is 96% for BOD and 76% for COD-Mn.
Claims (1)
して好気的に培養する事を特徴とする酵母による工場廃
液の浄化方法。1. A method for purifying factory waste liquid using yeast, which is characterized by inoculating yeast of the genus Trichosporon into amino acid fermentation waste liquid and culturing it aerobically.
Priority Applications (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP51077344A JPS5913276B2 (en) | 1976-06-30 | 1976-06-30 | Method for purifying factory waste liquid using yeast |
| GB26546/77A GB1528719A (en) | 1976-06-30 | 1977-06-24 | Method of waste water treatment by yeast |
| FR7719872A FR2356603A1 (en) | 1976-06-30 | 1977-06-28 | RESIDUAL WATER TREATMENT PROCESS BY YEASTS |
| ES460161A ES460161A1 (en) | 1976-06-30 | 1977-06-28 | Method of waste water treatment by yeast |
| MY190/81A MY8100190A (en) | 1976-06-30 | 1981-12-30 | Method of waste water treatment by yeast |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP51077344A JPS5913276B2 (en) | 1976-06-30 | 1976-06-30 | Method for purifying factory waste liquid using yeast |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS532949A JPS532949A (en) | 1978-01-12 |
| JPS5913276B2 true JPS5913276B2 (en) | 1984-03-28 |
Family
ID=13631294
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP51077344A Expired JPS5913276B2 (en) | 1976-06-30 | 1976-06-30 | Method for purifying factory waste liquid using yeast |
Country Status (5)
| Country | Link |
|---|---|
| JP (1) | JPS5913276B2 (en) |
| ES (1) | ES460161A1 (en) |
| FR (1) | FR2356603A1 (en) |
| GB (1) | GB1528719A (en) |
| MY (1) | MY8100190A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0210991Y2 (en) * | 1985-02-19 | 1990-03-19 |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6015397B2 (en) * | 1983-05-20 | 1985-04-19 | 名古屋大学長 | Method for treating wastewater containing phenols containing formaldehyde |
| CN113998832A (en) * | 2020-11-02 | 2022-02-01 | 呼伦贝尔东北阜丰生物科技有限公司 | Method for advanced treatment of total nitrogen in amino acid wastewater |
| CN112920958B (en) * | 2021-03-03 | 2022-09-16 | 江苏宜裕环保科技有限公司 | High-concentration wastewater biological pretreatment functional bacteria and application thereof |
| CN115282935A (en) * | 2022-08-16 | 2022-11-04 | 南京农业大学 | Preparation method and application of ferro-manganese modified yeast powder |
| CN116589148B (en) * | 2023-07-13 | 2023-09-22 | 临沂大驰水务有限公司 | Advanced sewage treatment method for producing erythromycin thiocyanate |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR1256076A (en) * | 1960-02-04 | 1961-03-17 | Landaise Des Celluloses Soc | Improvements to the biological purification of industrial or urban wastewater containing organic matter |
| JPS5113348B1 (en) * | 1968-04-27 | 1976-04-27 | ||
| SU344734A1 (en) * | 1970-10-19 | 1973-10-19 | Институт микробиологии Августа Кирхенштейка | METHOD OF OBTAINING L-LYSIN |
| FR2259060A1 (en) * | 1974-01-29 | 1975-08-22 | British Petroleum Co | Biological purification of effluent - esp contg phenolics and detergents using Trichosporon cutaneum |
-
1976
- 1976-06-30 JP JP51077344A patent/JPS5913276B2/en not_active Expired
-
1977
- 1977-06-24 GB GB26546/77A patent/GB1528719A/en not_active Expired
- 1977-06-28 ES ES460161A patent/ES460161A1/en not_active Expired
- 1977-06-28 FR FR7719872A patent/FR2356603A1/en active Granted
-
1981
- 1981-12-30 MY MY190/81A patent/MY8100190A/en unknown
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0210991Y2 (en) * | 1985-02-19 | 1990-03-19 |
Also Published As
| Publication number | Publication date |
|---|---|
| MY8100190A (en) | 1981-12-31 |
| FR2356603A1 (en) | 1978-01-27 |
| GB1528719A (en) | 1978-10-18 |
| FR2356603B1 (en) | 1983-08-26 |
| ES460161A1 (en) | 1978-05-16 |
| JPS532949A (en) | 1978-01-12 |
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