KR890000517A - 하이브리드 단백질 c 및 이의 제조방법 - Google Patents
하이브리드 단백질 c 및 이의 제조방법 Download PDFInfo
- Publication number
- KR890000517A KR890000517A KR1019880006968A KR880006968A KR890000517A KR 890000517 A KR890000517 A KR 890000517A KR 1019880006968 A KR1019880006968 A KR 1019880006968A KR 880006968 A KR880006968 A KR 880006968A KR 890000517 A KR890000517 A KR 890000517A
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- KR
- South Korea
- Prior art keywords
- factor
- amino acid
- hybrid protein
- human
- protein
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6464—Protein C (3.4.21.69)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/647—Blood coagulation factors not provided for in a preceding group or according to more than one of the proceeding groups
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21069—Protein C activated (3.4.21.69)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Hematology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Veterinary Medicine (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Public Health (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Animal Behavior & Ethology (AREA)
- Diabetes (AREA)
- Pharmacology & Pharmacy (AREA)
- Gastroenterology & Hepatology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
내용 없음
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제 1 도는 플라즈미드 pCS1의 제한지도를 나타낸 도이다.
제 2 도는 플라즈미드 pCS4의 제한지도를 나타낸 도이다.
제 3 도는 플라즈미드 pHSG293의 제한지도를 나타낸 도이다.
Claims (6)
1번부터 43번 잔기까지의 아미노산 서열이 사람 프로트롬빈, 인자 VII, 인자IX, 또는 인자 X의 1번부터 43번 잔기까지의 아미노산 서열로 치환된 사람 단백질 C의 하이브리드 단백질.
제 1 항에 있어서, 인자 X의 30번 발린(Valine) 잔기가 또한 이소류신(isoleucine)으로 치환된 하이브리드 단백질.
사람 단백질 C의 1번부터 43번 잔기까지의 아미노산 서열이 사람 프로트롬빈, 인자Ⅶ, 인자IX, 또는 인자 X의 1번부터 43번 잔기까지의 아미노산 서열로 치환된 사람 단백질 C의 하이브리드 단백질을 암호화하는 DNA.
제 3 항에 있어서, 사람 인자 X의 30번 발린 잔기가 또한 이소류신으로 치환된 하이브리드 단백질을 암호화하는 DNA.
사람 단백질 C의 1번부터 43번 잔기까지의 아미노산 서열이 사람 프로트롬빈, 인자Ⅶ, 인자IX, 또는 인자 X의 1번부터 43번 잔기까지의 아미노산 서열로 치환된 사람 단백질 C의 하이브리드 단백질을 암호화하는 DNA를 사용하여 하이브리드 단백질을 제조하는 방법.
제 5 항에 있어서, 인자 X의 30번 발린 잔기가 또한 이소류신으로 치환된 하이브리드 단백질을 암호화하는 DNA를 사용하여 하이브리드 단백질을 제조하는 방법.
※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP62-145293 | 1987-06-12 | ||
JP14529387 | 1987-06-12 |
Publications (1)
Publication Number | Publication Date |
---|---|
KR890000517A true KR890000517A (ko) | 1989-03-15 |
Family
ID=15381791
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1019880006968A KR890000517A (ko) | 1987-06-12 | 1988-06-10 | 하이브리드 단백질 c 및 이의 제조방법 |
Country Status (7)
Country | Link |
---|---|
EP (1) | EP0296413A2 (ko) |
JP (1) | JP2799316B2 (ko) |
KR (1) | KR890000517A (ko) |
AU (1) | AU1756588A (ko) |
DK (1) | DK317288A (ko) |
FI (1) | FI882746A (ko) |
PT (1) | PT87688B (ko) |
Families Citing this family (24)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5516650A (en) * | 1985-06-27 | 1996-05-14 | Zymogenetics, Inc. | Production of activated protein C |
JP2728240B2 (ja) * | 1988-07-26 | 1998-03-18 | ヘキスト薬品工業株式会社 | ヒトプロテインc変異体及びその製造方法 |
US5298599A (en) * | 1988-12-30 | 1994-03-29 | Oklahoma Medical Research Foundation | Expression and purification of recombinant soluble tissue factor |
GB8927722D0 (en) * | 1989-12-07 | 1990-02-07 | British Bio Technology | Proteins and nucleic acids |
JP3153236B2 (ja) * | 1989-12-22 | 2001-04-03 | ザイモジェネティクス,インコーポレイティド | 端が切り取られた軽鎖を有する組換えプロテインc |
US5225537A (en) * | 1989-12-29 | 1993-07-06 | Zymogenetics, Inc. | Methods for producing hybrid phospholipid-binding proteins |
EP0506821B1 (en) * | 1989-12-29 | 1998-02-04 | Zymogenetics, Inc. | Hybrid protein c |
ATE180834T1 (de) * | 1990-01-29 | 1999-06-15 | Zymogenetics Inc | Antikoagulierende proteine |
US5910481A (en) * | 1995-11-13 | 1999-06-08 | Immuno Ag | Hybrid proteins with modified activity |
AU730133B2 (en) * | 1996-11-08 | 2001-02-22 | Oklahoma Medical Research Foundation | Modified protein C and methods of use thereof |
US6693075B1 (en) | 1997-10-23 | 2004-02-17 | Regents Of The University Of Minnesota | Modified vitamin K-dependent polypeptides |
US7247708B2 (en) | 1997-10-23 | 2007-07-24 | Regents Of The University Of Minnesota | Modified vitamin K-dependent polypeptides |
US6747003B1 (en) | 1997-10-23 | 2004-06-08 | Regents Of The University Of Minnesota | Modified vitamin K-dependent polypeptides |
US6017882A (en) * | 1997-10-23 | 2000-01-25 | Regents Of The University Of Minnesota | Modified vitamin K-dependent polypeptides |
US6998122B1 (en) | 1999-04-30 | 2006-02-14 | Eli Lilly And Company | Protein C derivatives |
EP1255821B1 (en) | 2000-02-02 | 2004-12-29 | Eli Lilly And Company | Protein c derivatives |
AU2001232799A1 (en) | 2000-02-11 | 2001-08-20 | Eli Lilly And Company | Protein c derivatives |
RU2278123C2 (ru) | 2000-02-11 | 2006-06-20 | Максиджен Холдингз Лтд. | Молекулы, подобные фактору vii или viia |
US7220837B1 (en) | 2000-04-28 | 2007-05-22 | Regents Of The University Of Minnesota | Modified vitamin K-dependent polypeptides |
US7812132B2 (en) | 2000-04-28 | 2010-10-12 | Regents Of The University Of Minnesota | Modified vitamin K-dependent polypeptides |
US20030211094A1 (en) | 2001-06-26 | 2003-11-13 | Nelsestuen Gary L. | High molecular weight derivatives of vitamin k-dependent polypeptides |
SI1499719T1 (sl) | 2002-04-30 | 2011-03-31 | Bayer Healthcare Llc | Polipeptidne variante faktorja VII ali VIIa |
DK2085470T3 (da) | 2003-03-20 | 2012-08-06 | Bayer Healthcare Llc | FVII- eller FVIIa-varianter |
CN1839203B (zh) | 2003-06-19 | 2011-11-16 | 拜耳医药保健有限公司 | 因子VII或VIIa的GLA结构域变体 |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GR860984B (en) * | 1985-04-17 | 1986-08-18 | Zymogenetics Inc | Expression of factor vii and ix activities in mammalian cells |
US4959318A (en) * | 1985-06-27 | 1990-09-25 | Zymogenetics, Inc. | Expression of protein C |
EP0215548B2 (en) * | 1985-06-27 | 1998-01-07 | Zymogenetics, Inc. | Expression of protein C |
-
1988
- 1988-06-09 PT PT87688A patent/PT87688B/pt not_active IP Right Cessation
- 1988-06-09 JP JP63140558A patent/JP2799316B2/ja not_active Expired - Lifetime
- 1988-06-09 FI FI882746A patent/FI882746A/fi not_active Application Discontinuation
- 1988-06-09 EP EP88109186A patent/EP0296413A2/en not_active Withdrawn
- 1988-06-10 KR KR1019880006968A patent/KR890000517A/ko not_active Application Discontinuation
- 1988-06-10 DK DK317288A patent/DK317288A/da not_active Application Discontinuation
- 1988-06-10 AU AU17565/88A patent/AU1756588A/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
AU1756588A (en) | 1988-12-15 |
JPS6485096A (en) | 1989-03-30 |
FI882746A (fi) | 1988-12-13 |
DK317288A (da) | 1988-12-13 |
FI882746A0 (fi) | 1988-06-09 |
DK317288D0 (da) | 1988-06-10 |
PT87688B (pt) | 1992-09-30 |
PT87688A (pt) | 1988-07-01 |
EP0296413A2 (en) | 1988-12-28 |
JP2799316B2 (ja) | 1998-09-17 |
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Legal Events
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WITN | Application deemed withdrawn, e.g. because no request for examination was filed or no examination fee was paid |