KR20100136789A - Whitening cosmetic composition containing the extract of puer tea, black garlic and moutan radicis cortex - Google Patents
Whitening cosmetic composition containing the extract of puer tea, black garlic and moutan radicis cortex Download PDFInfo
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Abstract
Description
본 발명은 미백용 화장료 조성물에 관한 것으로, 더욱 상세하게는 보이차 추출물, 흑마늘 추출물 및 목단피 추출물을 함유하는 미백용 화장료 조성물에 관한 것이다.The present invention relates to a cosmetic composition for whitening, and more particularly to a cosmetic composition for whitening containing ivory tea extract, black garlic extract and bark skin extract.
생활 수준의 향상과 소득의 증대로 여성들뿐 아니라 남성들 또한 피부미용에 대한 관심이 더욱 높아졌고, 최근에는 자외선 노출에 의한 기미 및 주근깨에 대한미백 효과에 대해 관심이 높아지고 있다. Increasing living standards and rising incomes have increased interest in skin care for men as well as women. Recently, attention has been paid to the whitening effects of blemishes and freckles caused by UV exposure.
한편, 피부색을 결정하는 가장 중요한 인자는 멜라닌, 카로틴, 헤모글로빈 등이 있는데 이중 가장 중요한 것은 멜라닌이다. 멜라닌의 생합성은 아미노산의 일종인 티로신이 멜라노사이트의 멜라노좀에서 티로시나제(tyrosinase)에 의해 산화 되어 디하이드록시 페닐알라닌(dihydroxy phenylalanine)으로 전환되는 것을 시작으로 계속되는 일련의 산화과정을 거쳐 갈색(pheomelanin), 흑색(eumelanin)의 중합체로 형성된다. 이러한 생합성 과정은 멜라노좀이라는 특수한 형태의 갈색 세포내 소기관에서 진행되며 멜라닌 과립을 포함한 멜라노좀은 핵 주변 부위에서 수지상 돌기 끝부분으로 이동, 케라티노사이트의 식세포작용에 의해 세포질 내로 이동하고 이들은 케라티노사이트의 핵 주변에 축적된다. 멜라닌의 합성과 멜라노좀의 수, 주위 케라티노사이트로의 이동은 일차적으로 유전적인 영향을 받으며 중금속, 황사, 오존, 자외선 등과 같은 외부 환경과 호르몬의 분비 이상 등의 내부 환경의 영향을 받는다. 그 밖에 티로시나제의 발현 및 멜라닌의 합성, 전송에 관여하는 세포 내 조절인자인 싸이토카인(cytokine), 구리, 아연, 철 등의 금속 이온 및 인터페론, 프로스타글란딘, 히스타민 등도 영향을 주는 것으로 알려져 있다(박수남, 대한화장품학회지, 25: 77-127, 1999). On the other hand, the most important factors for determining the skin color are melanin, carotene, hemoglobin and the most important of these is melanin. The biosynthesis of melanin begins with a series of oxidation processes, starting with the conversion of tyrosine, an amino acid, from the melanocytes of melanocytes to tyrosinase, to dihydroxy phenylalanine. It is formed of a polymer of eumelanin. This biosynthesis process is carried out in a special type of brown intracellular organelles called melanosomes. Melanosomes, including melanin granules, move from the periphery of the nucleus to the dendritic end, and then into the cytoplasm by the phagocytosis of keratinocytes. Accumulate around the nucleus of the site. The synthesis of melanin, the number of melanosomes, and the migration to surrounding keratinocytes are primarily genetically influenced by the external environment, such as heavy metals, yellow dust, ozone, and ultraviolet rays, and by the internal environment such as abnormal hormone secretion. In addition, cytokines (cytokine), which are involved in the expression of tyrosinase, the synthesis and transfer of melanin, and metal ions such as copper, zinc and iron, as well as interferons, prostaglandins and histamines, are known to have an effect. Society of Cosmetic Scientists, 25: 77-127, 1999).
따라서, 피부색을 결정짓는 멜라닌의 합성을 억제하고 이에 관여하는 효소인 티로시나제의 활성을 억제함으로써 피부의 미백효과를 기대할 수 있다 할 것이다.Therefore, the skin whitening effect can be expected by inhibiting the synthesis of melanin, which determines the skin color, and the activity of tyrosinase, an enzyme involved therein.
한편, 국내의 수입 상가 등에서 불법으로 수입된 미백화장품들이 공공연히 판매되고 있어 그 유행성 여부가 의문시되고 있으며, 이러한 제품들은 주로 미백효과를 극대화하기 위해 인체에 유해한 수은이 다량으로 함유되어 있을 가능성이 있다. 수은이 다량으로 함유된 화장품을 사용할 경우, 수은 성분이 피부에 쉽게 흡수 되어 알레르기 반응을 일으킴으로 심한 가려움, 홍반, 부종 등이 발생되고, 장기간 사용할 때에는 체내에 축적되어 심한 부작용을 가져올 수 있다. 또한, 현재 시판되 고 있는 미백 화장품 소재의 경우 높은 수입의존도, 고가, 선진국 기업에 대한 낮은 경쟁력 때문에 실제로 적용할 수 있는 것은 많지 않은 문제점이 있다. On the other hand, whitening cosmetics imported illegally from domestic import stores, etc. are being sold publicly, and the epidemic is questioned. These products may contain a large amount of mercury harmful to the human body in order to maximize the whitening effect. When using cosmetics containing a large amount of mercury, mercury components are easily absorbed into the skin, causing allergic reactions, severe itching, erythema, swelling, etc., and accumulate in the body during long-term use can cause severe side effects. In addition, the whitening cosmetics materials currently on the market are problematic in that they cannot be actually applied because of high import dependency, high price, and low competitiveness of developed countries.
이에 본 발명은 국내에서 공급이 용이한 보이차 추출물, 흑마늘 추출물 및 목단피 추출물을 혼합한 천연소재 혼합추출물을 유효성분으로 함유함으로써 실질적인 미백효과를 발휘하는 미백용 화장료 조성물을 제공하는데 그 목적이 있다. Accordingly, the present invention has a purpose to provide a cosmetic composition for whitening exhibiting a substantial whitening effect by containing a natural material mixed extract of Boi tea extract, black garlic extract and bark skin extract which is easy to supply in Korea as an active ingredient.
상기의 목적을 달성하기 위해 본 발명은 보이차 추출물 1 중량부에 대해 흑마늘 추출물 0.5~2 중량부 및 목단피 추출물 0.5~2 중량부를 혼합한 혼합추출물을 유효성분으로 함유하는 것을 특징으로 하는 미백용 화장료 조성물을 제공한다.In order to achieve the above object, the present invention is a whitening cosmetic comprising a mixed extract of 0.5 to 2 parts by weight of black garlic extract and 0.5 to 2 parts by weight of bark skin extract as an active ingredient with respect to 1 part by weight of tea extract To provide a composition.
이하, 본 발명의 과제 해결 수단에 대해 상세히 설명하고자 한다. Hereinafter, the problem solving means of the present invention will be described in detail.
보이차는 중국차의 일종으로, 주로 중국 운남지방이 기원인 발효차를 의미한다. 주로 중국 운남성(雲南省)에서 자생하는 대엽종(大葉種) 차나무에서 얻어지며 차엽의 효소를 파괴시킨 뒤 차입을 퇴적하여 공기중에 있는 미생물의 번식을 유도해 다시 발효가 일어나게 만든 차이다. 차엽에 함유된 성분으로는 비타민을 비롯하여 카페인, 탄닌, 플라보노이드 및 정유 등이 있다(이용주 등, 생약학, 동명사. 189-190, 1981). 찻잎은 전통적으로 당뇨, 비만, 항산화, 항고혈압, 항균, 콜레스테롤 강하 및 항궤양작용 등을 나타내는 것으로 알려져 있어 상용차로서 일상적으로 음용되고 있다.Boi tea is a kind of Chinese tea, which means fermented tea mainly originated from Yunnan Province of China. It is mainly derived from the large-leafed tea tree growing in Yunnan Province, China. It destroys the enzymes of the tea leaves and deposits them to induce the reproduction of microorganisms in the air, causing the fermentation to occur again. Ingredients contained in tea leaves include caffeine, tannins, flavonoids and essential oils, including vitamins (Yongju Lee, et al., Pharmacology of the same name. 189-190, 1981). Tea leaves have traditionally been known to exhibit diabetes, obesity, antioxidant, antihypertensive, antibacterial, cholesterol lowering and anti-ulcer activity and are commonly used as commercial vehicles.
흑마늘은 생마늘을 일정기간 동안 알맞은 온도 및 습도 조건에서 숙성시켜 검은색으로 변한 것을 말한다 이러한 흑마늘은 생마늘에 비하여 항산화력이 현저하게 상승하고, 암 예방, 콜레스테롤 저하, 동맥경화개선, 심장질환의 예방 등의 효과가 생마늘보다 현저하게 증가되며, 폴리페놀류의 함량이 증가하여 생마늘에는 존재하지 않는 S-아릴시스테인이라는 수용성의 유황 아미노산이 생성된다.Black garlic refers to the change of black garlic after ripening it at a suitable temperature and humidity conditions for a certain period of time. This black garlic has a significant increase in antioxidant power, prevents cancer, lowers cholesterol, improves arteriosclerosis, and prevents heart disease. The effect of is significantly increased than raw garlic, and the content of polyphenols is increased to produce a water-soluble sulfur amino acid called S-arylcysteine which does not exist in raw garlic.
또한, 마늘을 숙성시킴으로써 냄새의 원인이 되는 휘발성 유황화합물이 줄어들기 때문에, 먹은 후에 입에서 마늘냄새가 몸속에서부터 진동하는 마늘 특유의 불쾌감이 전혀 없다는 특징이 있다.In addition, since volatile sulfur compounds that cause odors are reduced by ripening garlic, there is no characteristic discomfort that garlic peculiar to garlic vibrating from the body after eating.
목단피는 모란과(Paeoniaceae) 여러해살이풀 모란(Paeonia suffruticosaANDR.)의 뿌리껍질로, 한방에서는 소염·진통제로서 충수염·월경통·부스럼 항균작용, 혈압강하작용, 통경작용, 항염증작용, 위액분비억제작용, 진정작용 등의 치료에 사용하는 것으로 알려져 있다. 목단피에는 인중합체(polyphosphate), 파에오놀 (paeonol), 파에오노사이드 (paeonoside), 파에오놀라이드(paeonolide), 파에오니프로린(paeoniflorin), 파이토스텍롤 (phytosterol) 등의 우수한 생리활성을 가진 유효성분들이 많이 함유되어 있는 것으로 알려져 있다.Root bark is the root bark of Paeoniaceae perennial peony (Paeonia suffruticosa ANDR.), Which is an anti-inflammatory and analgesic agent in oriental medicine. Appendicitis / menstrual pain. It is known to be used for the treatment of sedation. Bark skin has an excellent physiological activity such as polyphosphate, paeonol, paeonoside, paeonolide, paeoniflorin, phytosterol, etc. It is known to contain a lot of people.
한편, 본 발명은 상기의 천연소재인 보이차 추출물, 흑마늘 추출물 및 목단피 추출물을 혼합한 혼합추출물이 어떠한 작용을 통해 미백 효과를 발휘하는지 알아보기 위해 하기 실험예를 실시한 결과, 생체내에서 티로신(tyrosine)이라는 물질의 산화과정을 촉진하여 멜라닌의 생성을 도와주는 티로시나제의 활성을 억제하면 서 세포내 멜라닌의 생성을 억제함으로써 미백효과를 발휘하는 것을 확인할 수 있었다. On the other hand, the present invention was carried out the following experimental example to find out the effect of the mixed extract of the mixture of Bocha tea extract, black garlic extract and bark skin extract that is a natural material, the tyrosine (tyrosine in vivo) Inhibiting the activity of tyrosinase, which promotes the production of melanin by promoting the oxidation process of), it can be confirmed that the whitening effect by inhibiting the production of melanin in the cell.
한편, 본 발명의 미백용 화장료 조성물은 반드시 보이차 추출물 1 중량부에 대해 흑마늘 추출물 0.5~2 중량부 및 목단피 추출물 0.5~2 중량부를 혼합한 혼합추출물을 유효성분으로 함유하는데, 각 추출물들의 배합비는 티로시나제의 Km값에 의거하여 산출된 것으로 우수한 티로시나제 활성 억제 및 세포의 멜라닌 생성의 억제 효과를 발휘하기 때문이다. On the other hand, the cosmetic composition for whitening of the present invention necessarily contains a mixed extract of 0.5 ~ 2 parts by weight of black garlic extract and 0.5 ~ 2 parts by weight of bark skin extract with respect to 1 part by weight of tea tea extract as an active ingredient, the blending ratio of each extract It is calculated based on the Km value of tyrosinase and is because it exhibits an excellent inhibitory effect on tyrosinase activity and melanin production of cells.
한편, 보이차 추출물, 흑마늘 추출물 또는 목단피 추출물 중 선택되는 어느 하나 이상의 추출물은 바람직하게 보이차, 흑마늘 또는 목단피 중 선택되는 어느 하나의 건체 시료 1중량부에 대해 증류수 8.7~12.7 중량부를 가하고 50~70℃에서 20~28시간 쉐이킹 인큐베이션(shaking incubation)을 한 후, 5000~7000rpm으로 50~70분 동안 원심분리를 하고 0.35~0.55㎛로 여과하여 수득한 여과액을 동결건조함으로써 제조되는 것이 좋다.On the other hand, any one or more extracts selected from ivory tea extract, black garlic extract or bark skin extract is preferably added 8.7 to 12.7 parts by weight of distilled water to 1 part by weight of any dry sample selected from ivory tea, black garlic or bark skin and 50 to 70 Shaking incubation (shaking incubation) at 20 ℃ for 28 hours, centrifugation for 50 to 70 minutes at 5000 ~ 7000rpm and is preferably prepared by lyophilizing the filtrate obtained by filtration to 0.35 ~ 0.55㎛.
한편, 본 발명의 미백용 화장료 조성물의 제형은 바람직하게 화장수, 젤, 수용성 리퀴드, 크림, 에센스, 수중유(O/W)형 및 유중수(W/O)형으로 이루어진 기초화장료 제형, 연고, 수중유형 및 유중수형 메이크업베이스, 파운데이션, 스킨커버, 립스틱, 립그로스, 페이스파우더, 투웨이케익, 아이새도, 치크칼라 및 아이브로우 펜슬류로 이루어진 색조화장료 제형 중에서 선택되는 어느 하나인 것이 좋다.On the other hand, the formulation of the cosmetic composition for whitening of the present invention is preferably a cosmetic composition, ointment, consisting of a lotion, gel, water-soluble liquid, cream, essence, oil-in-water (O / W) type and water-in-oil (W / O) type. The oil-in-water type and the water-in-oil makeup base, foundation, skin cover, lipstick, lip gloss, face powder, two-way cake, eye shadow, cheek color and eyebrow pencils are selected from any one of the color cosmetic formulation.
상기에서 살펴본 바와 같이 본 발명의 미백용 화장료 조성물은 생체내에서 티로신(tyrosine)이라는 물질의 산화과정을 촉진하여 멜라닌의 생성을 도와주는 티로시나제의 활성을 억제하면서 세포내 멜라닌의 생성을 억제하는 천연소재 혼합추출물을 유효성분으로 함유함으로써 실질적인 미백효과를 발휘한다.As described above, the cosmetic composition for whitening of the present invention is a natural material that inhibits the production of intracellular melanin while inhibiting the activity of tyrosinase, which promotes the oxidation process of a tyrosine (tyrosine) substance to help melanin production in vivo. By containing the mixed extract as an active ingredient, it shows a substantial whitening effect.
또한, 본 발명은 천연 소재들로부터 유래된 혼합추출물을 함유함으로써 화학성분을 포함하는 화장료 조성물에 대한 소비자들의 거부감 및 불쾌감을 감소시킬 수 있다. In addition, the present invention can reduce the consumer's discomfort and discomfort for the cosmetic composition comprising a chemical component by containing a mixed extract derived from natural materials.
이하, 본 발명의 구성 및 작용에 대해 하기 실시예에서 더욱 상세히 설명하지만, 본 발명의 권리범위가 하기 실시예에만 한정되는 것은 아니고, 이와 등가의 기술적 사상의 변형까지를 포함한다.Hereinafter, the configuration and operation of the present invention will be described in more detail with reference to the following examples, but the scope of the present invention is not limited to the following examples, and includes modifications of equivalent technical spirit.
실시예Example 1: 천연소재 혼합추출물 제조 1: Manufacture of natural extracts
보이차 잎, 흑마늘, 목단피 각각의 추출물을 제조하기 위해 각각의 건체 20g에 증류수 214ml을 첨가한 후, 60℃에서 24시간 쉐이킹 인큐베이션한 다음, 6,000rpm에서 1시간 동안 원심분리하였다. 원심분리하여 수득된 각각의 추출물을 0.45㎛로 여과한 후 동결건조하여 각각의 분말을 수득하였다. 수득된 분말 각각을 500ppm이 되도록 증류수와 혼합하여 제조된 각각의 추출물을 1:1:1로 혼합하여 천연소재 혼합추출물을 제조하였다. To prepare extracts of black tea leaves, black garlic, and bark skin, 214 ml of distilled water was added to 20 g of each dried body, followed by shaking incubation at 60 ° C. for 24 hours, followed by centrifugation at 6,000 rpm for 1 hour. Each extract obtained by centrifugation was filtered to 0.45 mu m and then lyophilized to obtain each powder. Each of the powders obtained was mixed with distilled water so as to be 500ppm, each of the extracts were mixed in a 1: 1: 1 to prepare a natural material mixed extract.
실험예Experimental Example 1: 세포 생존율 측정 1: Cell viability measurement
본 실험예 1은 상기 실시예 1에서 수득한 천연소재 혼합추출물에 대한 세포 생존율을 측정하였다.Experimental Example 1 measured the cell viability of the natural material mixture extract obtained in Example 1.
배양이 끝난 B16 세포의 생존율은 Chung 등이 사용한 MTT(3-(4,5-dimethythiazol-2-yl)-2,5-dipheyl terazolium bromid)환원 방법을 이용하여 측정하였다.The viability of the cultured B16 cells was measured using the reduction method of MTT (3- (4,5-dimethythiazol-2-yl) -2,5-dipheyl terazolium bromid) used by Chung et al.
B16 세포를 준비한 각 웰에 천연소재 혼합추출물 500ppm을 투여하고 배양하였다. 그리고 각 웰(well)에 MTT 용액(5㎎/㎖)을 성장배지의 10분의 1을 가해주고 다시 37℃에서 4시간 더 배양하여 MTT를 환원시킴으로써 생성된 포르마존(formazon)이 배지에 따라나가지 않도록 배지를 조심스럽게 제거하였다. 남아 있는 배지를 완전히 제거하기 위해 실온에서 30분간 방치한 후 DMSO를 이용하여 용해시킨 실시예 1을 570nm에서 흡광도를 측정하였다. 흡광도 측정시 공시료는 DMSO로 하였고, 세포의 생존율은 하기 수학식 1에 의해 계산되었다. Each well prepared with B16 cells was administered with 500ppm of the natural material mixture extract and incubated. In addition to each well (5mg / ㎖) MTT solution (10mg) of the growth medium and incubated for 4 hours at 37 ℃ again to reduce the MTT produced formazon (formazon) according to the medium The medium was carefully removed so as not to exit. In order to completely remove the remaining medium, the absorbance was measured at 570 nm of Example 1, which was left at room temperature for 30 minutes and dissolved using DMSO. When the absorbance was measured, the test sample was DMSO, and cell viability was calculated by Equation 1 below.
측정결과(도 1), 대조군의 세포 생존율을 100%로 보았을 때, 천연소재 혼합추출물의 세포 생존율은 91.808%로 세포 생존율에 큰 영향을 미치지 않음을 확인할 수 있었다. As a result of the measurement (FIG. 1), when the cell viability of the control group was 100%, the cell viability of the natural material mixture extract was 91.808%, which did not significantly affect the cell viability.
실험예Experimental Example 2: 2: 티로시나제Tyrosinase (( TyrosinaseTyrosinase ) 활성 억제() Suppress activity ( cellcell -- freefree systemsystem ) 정도 측정A) degree measurement
본 실험예 2에서는 실시예 1에서 수득한 천연소재 혼합추출물의 미백효과를 확인하기 위해 티로시나제(tyrosinase)의 활성 억제 정도를 측정하였다. In Experimental Example 2 to determine the whitening effect of the natural material mixture extract obtained in Example 1 was measured the degree of activity inhibition of tyrosinase (tyrosinase).
티로시나제는 생체내에서 티로신(tyrosine)이라는 물질의 산화과정을 촉진하여 멜라닌이 생성되게 도와주는 효소이다. Tyrosinase is an enzyme that accelerates the oxidation of tyrosine in vivo and helps melanin production.
티로시나제 활성 억제 정도를 측정하기 위해 효소활성 저해능 측정 도파옥시데이션(dopaoxidation) 방법을 사용하였다. 이는 도파(dopa)를 기질로 하여 티로시나제(tyrosinase)에 의하여 생성되는 반응 산물인 도파크롬(dopachrome)이 475nm에서 흡광도를 나타내는 점을 이용하였다. In order to measure the degree of inhibition of tyrosinase activity, a dopaoxidation method was used. The dopachrome, a product of reaction produced by tyrosinase, was absorbed at 475 nm.
35℃ 수조에서 0.1M 포스페이트 버퍼(phosphate buffer; pH 6.8) 0.2㎖, 5mM L-DOPA 용액(solution) 0.2㎖ 및 천연소재 혼합추출물(실시예 1) 0.5㎖의 혼합액에 티로시나제(tyrosinase; 250unit/㎖) 0.1㎖를 첨가하여 35℃에서 2분간 반응시킨 다음 475 nm에서 흡광을 측정하였다.Tyrosinase (250 units / ml) in a mixed solution of 0.2 ml of 0.1 M phosphate buffer (pH 6.8), 0.2 ml of 5 mM L-DOPA solution and 0.5 ml of natural material extract (Example 1) in a 35 ° C. water bath ) 0.1 ml was added and reacted at 35 ° C. for 2 minutes, and then absorbance was measured at 475 nm.
측정결과(도 2), 대조군에 비해 실시예 1의 티로시나제 활성이 낮은 것을 확인할 수 있었고, 이로부터 본 발명에 유효성분으로 함유되는 보이차 추출물, 흑마늘 추출물 및 목단피 추출물을 혼합한 혼합추출물은 티로시나제의 활성을 억제한다는 사실을 확인할 수 있었다. As a result of the measurement (FIG. 2), it was confirmed that the tyrosinase activity of Example 1 was lower than that of the control group. From this, the mixed extract of the ivory extract, the black garlic extract, and the bark extract contained as an active ingredient of the present invention were tyrosinase. It was confirmed that the activity is inhibited.
상기의 결과로부터 티로시나제의 활성을 억제하는 혼합추출물을 유효성분으로 함유하는 본 발명의 미백효과를 추론할 수 있었다.From the above results, the whitening effect of the present invention containing a mixed extract that inhibits the activity of tyrosinase as an active ingredient could be inferred.
실험예Experimental Example 3: 멜라닌 함량 측정 3: Determination of melanin content
본 실험예 3에서는 실시예 1에서 수득한 천연소재 혼합추출물의 미백효과를 확인하기 위해 B16 멜라노마셀(melanoma cells)의 멜라닌 함량을 측정하였다. In Experimental Example 3, the melanin content of B16 melanoma cells (melanoma cells) was measured to confirm the whitening effect of the natural material mixture extract obtained in Example 1.
B16 멜라노마 셀(melanoma cells)을 24-웰 프레이트(well plates)에 1×105 으로 분주한 뒤 24시간 동안 배양한 후 천연소재 혼합추출물 500ppm을 처리하여 24시간 동안 배양하였다. 배양 후, 배지에 함유된 천연소재 혼합추출물(실시예 1)을 제거한 다음, 1N NaOH를 100㎕ 분주하여 24시간 동안 세포를 녹인 후 450nm에서 흡광도를 측정하였다.B16 melanoma cells (melanoma cells) were dispensed into 24-well plates at 1 × 10 5 and incubated for 24 hours, followed by incubation for 24 hours by treatment with 500 ppm of the natural material mixed extract. After incubation, the natural material mixed extract contained in the medium (Example 1) was removed, and then 100 μl of 1N NaOH was dissolved in cells for 24 hours, and then absorbance was measured at 450 nm.
측정결과(도 3), 대조군에 비해 실시예 1을 처리한 셀의 멜라닌 함량이 낮은 것을 확인할 수 있었고, 이로부터 본 발명에 유효성분으로 함유되는 보이차 추출물, 흑마늘 추출물 및 목단피 추출물을 혼합한 혼합추출물은 멜라닌의 생성을 억제한다는 사실을 확인할 수 있었다. As a result of the measurement (FIG. 3), it was confirmed that the melanin content of the cell treated with Example 1 was lower than that of the control group, and from this, a mixture of ivory tea extract, black garlic extract, and bark peel extract contained as an active ingredient in the present invention. It was confirmed that the extract inhibits the production of melanin.
상기의 결과로부터 멜라닌의 생성을 억제하는 혼합추출물을 유효성분으로 함유하는 본 발명의 미백효과를 추론할 수 있었다.From the above results, it was possible to infer the whitening effect of the present invention containing a mixed extract that suppresses the production of melanin as an active ingredient.
도 1은 보이차 추출물, 흑마늘 추출물 및 목단피 추출물을 혼합한 혼합추출물의 세포 생존율을 MTT assay를 이용하여 측정한 도이다. 1 is a diagram showing the cell viability of the mixed extract of the tea extract, black garlic extract and bark extract using MTT assay.
도 2는 보이차 추출물, 흑마늘 추출물 및 목단피 추출물을 혼합한 혼합추출물의 티로시나제 활성 억제 효과를 나타낸 도이다.Figure 2 is a diagram showing the tyrosinase activity inhibitory effect of the mixture extract of Bocha tea extract, black garlic extract and bark skin extract.
도 3은 보이차 추출물, 흑마늘 추출물 및 목단피 추출물을 혼합한 혼합추출물을 처리한 B16 멜라노나셀에서 멜라닌의 함량을 나타낸 도이다.Figure 3 is a diagram showing the content of melanin in B16 melanonacell treated with a mixed extract of tea tea extract, black garlic extract and bark skin extract.
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| CN111840189A (en) * | 2020-09-10 | 2020-10-30 | 山东钰财农业发展有限公司 | Production process of black garlic moisturizing cream |
| KR20210077388A (en) * | 2019-12-17 | 2021-06-25 | 최해연 | Method for manufacturing Liquid concentrate of Dark tea and Liquid concentrate of Dark tea using the same |
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| KR102224312B1 (en) | 2014-06-30 | 2021-03-09 | (주)아모레퍼시픽 | Composition for skin whitening comprising ostruthium (masterwort) extract |
| KR102224313B1 (en) | 2014-06-30 | 2021-03-09 | (주)아모레퍼시픽 | Composition for skin whitening comprising scutellaria alpina extract |
| KR20160091037A (en) | 2015-01-23 | 2016-08-02 | (주)아모레퍼시픽 | Composition comprising gooseberry or glutathione |
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| JP4907477B2 (en) | 2007-09-14 | 2012-03-28 | 株式会社スパン・ライフ | Black garlic manufacturing method and food containing black garlic |
| KR100895615B1 (en) * | 2008-03-12 | 2009-05-06 | (주)에이지아이 | Preparation of fermented garlic extract and composition containing the same |
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| KR20210077388A (en) * | 2019-12-17 | 2021-06-25 | 최해연 | Method for manufacturing Liquid concentrate of Dark tea and Liquid concentrate of Dark tea using the same |
| CN111840189A (en) * | 2020-09-10 | 2020-10-30 | 山东钰财农业发展有限公司 | Production process of black garlic moisturizing cream |
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